CN111073972B - Application of MYH6 gene in congenital heart disease diagnosis product - Google Patents

Abstract

The invention provides an application of a MYH6 gene in a congenital heart disease diagnosis product, and particularly relates to an application of a MYH6 gene, an expression level of the MYH6 gene, a MYH6 mutant gene or a mutation of an expression product of the MYH6 gene in a congenital heart disease diagnosis product; the invention also provides a congenital heart disease model, and further proves the relationship between the MYH6 gene and the congenital heart disease through experiments.

Description

Application of MYH6 gene in congenital heart disease diagnosis product

Technical Field

The invention relates to the field of gene application, in particular to application of a MYH6 gene in a congenital heart disease diagnosis product.

Background

Congenital Heart Disease (CHD) refers to heart and large blood vessels that exhibit abnormal anatomical structures after birth due to abnormal occurrence and development of the heart and large blood vessels during embryonic development. CHD is the most common birth defect in China at present, has the morbidity of about 0.6-0.8 percent and accounts for 1/5-1/4 of all birth defects. CHD not only causes physical and psychological pains to patients, but also causes great economic stress and social burden to families and society.

Atrial Septal Defects (ASD) are the most common congenital heart disease, accounting for about one-fourth of the incidence of CHD. Among them, secondary foramen atrial septal defect (ASD ii) is one of the most common types of ASD. ASDII defects are generally located within the fossa ovalis and manifest as persistent secondary atrial openings. Normal left atrial pressure (8-10 mmHg) is slightly higher than the right atrium (3-5 mmHg). Blood is shunted from left to right through the atrial septal defect. Shunting results in an increase in right ventricular volume loading, resulting in enlargement of the right atrium, right ventricle, and pulmonary artery dilation. In the early stage of pulmonary arteriolar spasm, cells of the wall of the pulmonary arteriolar gradually proliferate and the wall of the pulmonary arteriolar gradually thickens with the lapse of time to form resistance pulmonary hypertension. When the right atrial pressure is higher than the left atrium, blood is shunted from right to left, causing cyanosis, i.e., eisenmenger syndrome. Asdii can develop in isolation, can coexist with other intracardiac malformations, and can be included in the syndrome.

The MYH6 gene encodes the Myosin-6 protein within the myocardial sarcomere (myocardial heavy chain protein. Alpha.). MYH6 protein structurally comprises a head motor domain (1-780 AA), which binds to actin protein and has Mg2+ -ATPase activity; a neck region (781-840 AA); a rod-shaped tail region (841-1938 AA). The current research shows that MYH6 gene mutation is related to more serious diseases such as dilated cardiomyopathy, hypertrophic cardiomyopathy and sick sinus syndrome besides ASD, and MYH6 mutation causing the serious diseases is mainly located in the head region, while a series of pathogenic mutations related to ASD II are mostly located in the tail region.

Fetal cardiac ultrasound is currently commonly used for fetal CHD diagnosis. However, during fetal life, the secondary interatrial foramen is continuously open, and thus asdii can only be diagnosed after birth. Some fetal patients with clinical syndromes, particularly cardiomyopathy, mental retardation, critical limb deformities, etc., are highly suspected and, if they can be detected early in the fetus, can take early steps to perform cardiac surgery during the fetal period or to avoid the fetus from being born. The method has definite familial hereditary history, and can also adopt means such as test tube babies and the like according to the detection result of parents to block the next generation of hereditary same diseases. Furthermore, it would be of great interest for MYH6 mutations to determine early if they are associated with ASD or cardiomyopathy.

The three points all provide practical requirements for the early detection, early diagnosis and differential diagnosis, early treatment and prognosis judgment of the ASDII in the fetal period. Therefore, the exploration of key molecules of ASDII has important significance in defining the molecular mechanism of atrial septal occurrence and performing auxiliary molecular diagnosis by taking the mechanism as a target, and is beneficial to early discovery, early diagnosis and early treatment.

Disclosure of Invention

An object of the present invention is to provide a gene related to CHD.

The invention also aims to provide an application of a preparation, a chip or a kit for detecting MYH6 gene, expression level of MYH6 gene, MYH6 mutant gene or mutation of MYH6 gene expression product in diagnosis of congenital heart disease.

The invention also aims to provide a cell model and application thereof in MYH6 diagnosis and treatment.

In order to achieve the purpose, the invention adopts the following technical scheme:

the MYH6 mutant gene has a nucleotide sequence shown as SEQ ID NO.1, and compared with a normal MYH6 nucleotide sequence, mutation of the nucleotide at position 1576 is changed from G to A; an expression product MYH6 mutant protein of the MYH6 mutant gene has an amino acid sequence shown in SEQ ID No.2, and compared with a normal MYH6 amino acid sequence, the amino acid sequence is changed from Glu to Lys at the 526 th site, namely the glutamic acid is mutated into lysine.

In order to analyze the relationship between CHD and MYH6 mutant genes, the invention firstly uses Sanger sequencing method to verify the mutation sites of MYH6 genes of partial congenital heart disease patients and parents thereof, and verifies the congenital heart disease by cardiac ultrasound, digital Subtraction Angiography (DSA), magnetic resonance imaging/angiography or computed tomography.

The second aspect of the invention provides for the first time the use of a MYH6 gene in a product for the detection of congenital heart disease.

Further, the product comprises a reagent for detecting MYH6 gene, expression level of MYH6 gene, MYH6 mutant gene or mutation of MYH6 gene expression product in a biological sample to detect congenital heart disease.

Further, the detection method of the product is at least one of direct nucleic acid sequencing, nucleic acid molecule hybridization technology, nucleic acid amplification technology, nucleic acid probe technology or immunodetection.

Further, the product comprises a formulation, a chip or a kit.

Further, the detection sample of the product is peripheral blood.

Further, in the nucleic acid amplification technology, primers having nucleotide sequences shown as SEQ ID NO.4 and SEQ ID NO.5 are used.

The third aspect of the invention provides a kit for diagnosing ASD, which comprises a detection reagent for specifically recognizing the 1576 th nucleotide site of the MYH6 gene or specifically recognizing and detecting the 526 th amino acid site of the MYH6 protein.

Further, the detection reagent is a probe with a nucleotide sequence shown in SEQ ID NO.3, and is complementary with nucleotides 1562-1581 in MYH6 gene (GCATTGACCTCCATCGAGAAG).

Further, the probe is SYBR Green I, taqman probe, molecular beacon, hybridization probe or composite probe

In a fourth aspect of the invention, a method of constructing an animal model of congenital heart disease is provided, wherein a MYH6 gene mutation reagent is administered to a culture system, wherein the culture system comprises a cell system, a subcellular system, a solution system, a tissue system, an organ system, or an animal system.

Further, the MYH6 gene mutation reagent is MYH6 adenovirus.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, it is obvious that the drawings in the following description are only one embodiment of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 shows the results of sequencing in a patient family;

FIG. 2 is ATPase activity of MYH 6-WT and MYH6 780-E526K;

fig. 3 is a schematic diagram of myofilament structure.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The MYH6 mutant gene comprises a MYH6 gene subjected to base substitution mutation, frame shift mutation, deletion mutation and insertion mutation, and the mutation of an expression product of the MYH6 gene comprises an expression product of the MYH6 mutant gene.

Methods for detecting expression levels of MYH6 include, but are not limited to: polymerase Chain Reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), transcription Mediated Amplification (TMA), ligase Chain Reaction (LCR), strand Displacement Amplification (SDA) and Nucleic Acid Sequence Based Amplification (NASBA), in Situ Hybridization (ISH), chromosomal microarray expression profiling, southern or Northern blotting, sandwich ELISA, radioimmunoassay (RIA), direct, indirect or contrast enzyme-linked immunosorbent assay (ELISA), enzyme Immunoassay (EIA), fluorescence Immunoassay (FIA), western blotting, immunoprecipitation, and any particle-based immunoassay (e.g. using gold, silver or latex particles, magnetic particles or quantum dots). Among them, PCR requires reverse transcription of RNA into DNA before amplification (RT-PCR), TMA and NASBA to directly amplify RNA. Detecting the expression level of a gene "refers to determining the presence of mRNA of a marker gene in a biological sample and its expression level in order to predict CHD occurrence and can be accomplished by measuring the amount of mRNA. Analytical methods for this purpose are, but are not limited to, RT-PCR, competitive RT-PCR, real-time RTPCR, RNase Protection Assay (RPA), northern blotting, DNA microarray chips, etc.

Methods for detecting gene mutations or protein mutations include, but are not limited to: PCR-SSCP method, heteroduplex analysis method, denaturation gradient gel electrophoresis method, chemical cutting mismatch method, allele specific oligonucleotide analysis method, DNA chip technology, ligase chain reaction, allele specific amplification method, direct sequencing method.

In the present invention, the formulation can determine the presence of mRNA in a sample on a test strip, membrane, chip, disk, test strip, filter, microsphere, slide, multiwell plate, or fiber optic. The assay system can have a solid support to which nucleic acids corresponding to mRNA are attached. The solid support may comprise, for example, a plastic, a silicon wafer, a metal, a resin, a glass, a membrane, a particle, a precipitate, a gel, a polymer, a flake, a sphere, a polysaccharide, a capillary, a film, a plate, or a slide. The assay components can be packaged together after manufacture as a kit for detecting mRNA.

Nucleic acid hybridization techniques of the invention include, but are not limited to, in situ hybridization (FISH), chromosomal microarrays, and Southern or Northern blots. In Situ Hybridization (FISH) is a hybridization of specific DNA or RNA sequences in a tissue section or section using a labeled complementary DNA or RNA strand as a probe (in situ) or in the entire tissue if the tissue is small enough (whole tissue embedded ISH). DNAISH can be used to determine the structure of chromosomes. Rnash is used to measure and locate mRNA and other transcripts (e.g., ncRNA) within tissue sections or whole tissue embedding. Sample cells and tissues are typically treated to fix the target transcript in situ and to increase probe access. The probe is hybridized to the target sequence at high temperature, and then excess probe is washed away. The localization and quantification of base-labeled probes in tissues labeled with radiation, fluorescence or antigens is performed using autoradiography, fluorescence microscopy or immunohistochemistry, respectively. ISH can also use two or more probes labeled with radioactive or other non-radioactive labels to detect two or more transcripts simultaneously.

In the present invention, the immunoassay can be based on any of the following methods:

immunoprecipitation (Immunoprecipitation): immunoprecipitation is the simplest immunoassay method; this method measures the amount of precipitate that is formed after the reagent antibody has been incubated with the sample and reacted with the target antigen present therein to form insoluble aggregates. The immunoprecipitation can be either qualitative or quantitative.

Particle immunoassay: in a particle immunoassay, multiple antibodies are attached to the particle and the particle is capable of binding many antigenic molecules simultaneously. This greatly accelerates the speed of the visible reaction. This allows for a fast and sensitive detection of the biomarker.

Immunoturbidimetry (immunonephelometry): in immunoturbidimetry, the interaction of the antibody and the target antigen on the biomarker causes the formation of an immune complex that is too small to precipitate. However, these complexes will scatter incident light, which can be measured using a turbidimeter. The concentration of the antigen (i.e., biomarker) can be determined within minutes of the reaction.

Radioimmunoassay (RIA): radioimmunoassays use radioisotopes such as I125 to label antigens or antibodies. The isotope used emits gamma rays, which are usually measured after removal of unbound (free) radiolabel. The main advantages of RIA compared to other immunoassays are higher sensitivity, easy signal detection and confirmation, fast assay. The main disadvantages are the health and safety risks posed by the use of radiation and the time and expense associated with maintaining licensed radiation safety and disposal procedures. For this reason, RIA has been largely replaced by enzyme immunoassays in routine clinical laboratory practice.

Enzyme Immunoassay (EIA): enzyme immunoassays use enzymes to label antibodies or target antigens. The sensitivity of EIA is close to that of RIA without the risk of being caused by radioisotopes. One of the most widely used EIA methods for detection is enzyme-linked immunosorbent assay (ELISA). The ELISA method may use two antibodies, one specific for the target antigen and the other coupled to an enzyme, the addition of an enzyme substrate causing the generation of a chemiluminescent signal or a fluorescent signal.

Fluorescence Immunoassay (FIA): fluorescence immunoassay refers to an immunoassay that uses a fluorescent label or an enzyme label that acts on a substrate to form a fluorescent product. Fluorescence measurements are inherently more sensitive than chromatic (spectrophotometric) measurements. Thus, the FIA method has higher analytical sensitivity than the EIA method using absorption (optical density) measurement.

Chemiluminescence immunoassay: chemiluminescent immunoassays (CLIA) use a chemiluminescent label that produces light when excited by chemical energy; the emission is measured using a photodetector.

1. Validation of MYH6 mutations by Whole exome sequencing and Sanger sequencing

1.1 sample Collection

ASD families were collected at the national hospitals of the Guangdong province during the 11 th to 2018 th months from 2015. The diagnosis of ASD is confirmed by cardiac ultrasound, digital Subtraction Angiography (DSA), magnetic resonance imaging/angiography, or computed tomography. All parents underwent physical examination to confirm clinical performance.

The study was approved by the ethical committee of the people's hospital of Guangdong province. The patient or his parent sign informed consent.

1.2 Whole exome sequencing

1.2.1 extraction of DNA

The whole genome DNA of the peripheral blood of a patient and parents is extracted by using a blood genome DNA extraction kit of Tiangen, and the DNA is quantitatively detected by using 1 percent agarose gel electrophoresis and a Nanodrop 2000 spectrophotometer.

1.2.2 Whole exome sequencing and mutation Annotation

The genomic DNA was randomly fragmented into fragments of 200-300bp in length using a sonicator (Covaris 2, massachusetts, USA), 500ng of the purified DNA fragments were subjected to end repair and ligated with labeled linkers to construct a DNA sequencing library. The 3-step enzymatic reaction was performed according to the illumina standard library construction method: the sample library was formed by end repair, addition of "a" and ligation of Illumina sequencing adaptors (by PCR reaction, an 8bp barcode was ligated to the DNA fragment). Exon capture was performed using SureSelect Human All Exon V6+ UTR r2core design (91mb, agilent), the specific procedure is described in the instructions.

Sequencing was performed using the Illumina HiSeq X-tenstform platform, SNP sites and indel markers were detected using gattk v3.5, and annotation was performed using ANNOVAR.

The conservation and pathogenicity of candidate variations are predicted using bioinformatic prediction tools. All variants were screened for pathogenic variants against the public databases 1000 genes Project (http:// www. International genome. Org /), genome variant server, NHLBI GO Entity Sequencing Project (ESP) http:// EVS. Gs. Washington. Edu/EVS /) and genome Aggregation Consortium (ExAC) (http:// ExAC. Broadassociation. Org /).

1.2.3 Sanger sequencing validation

Mutation sites were verified using Sanger sequencing and Sanger sequencing was performed on an ABI3730 sequencer. MYH6 primer has nucleotide sequence shown as SEQ ID NO.4 and SEQ ID NO. 5.

1.3 results

The results are shown in FIG. 1:

(A) Both families carry the MYH6 p.E526K mutation, are autosomal dominant inheritance, and accord with complete cosegregation.

(B) In both families, patients carried the mutation, and neither member of the normal family carried the mutation.

(C) Conservation analysis, indicated by the arrow, the 526 site is highly conserved across species.

Whole exome sequencing results showed that the patient harbored the MYH6 gene variation NM — 002471c.g1576ap.e526k, while the normal member of the pedigree did not carry this mutation.

ATPase Activity detection

2.1 cell lines

C2C12 cell line

2.2 construction of vectors

The cDNA coding human MYH6 (1-780 AA) truncated MYH 6-WT and mutant MYH6780-E526K thereof are amplified by polymerase chain reaction and verified by DNA sequencing. These sequences were cloned into the vector GV362 (genechem) with 3Flag-tag at the XhoI/BamHI position.

2.3 transfection and determination of Mg2+ -atp enzyme Activity

Actin-activated Mg2+ -ATPase activity was measured spectrophotometrically at 37 ℃. C2C12 cells were transfected with MYH 6-WT and MYH 6-E780-526K, respectively. 48 hours after transfection, cells were collected and counted using a cytometer auto 1000 counter (nexcelom bioscience). Total ATPase activity was determined in a medium consisting of 50mmol Tris-HCl, pH 7.4, 120mmol NaCl, 20mmol KCl, 4mmol MgCl2, 240mmol sucrose, 1mmol potassium ethylenediaminetetraacetate (K + -TDNF), 3mMATP disodium and 104 cells (final volume of 1 ml). 1mmol of quinacrine was added to determine the activity of Mg2+ -ATPase. To 0.9mmol of sulfuric acid was added 2ml of a mixture of 1% lubrauol and 1% ammonium molybdate, and the reaction was stopped after 20 minutes. After development, the color was yellow and the wavelength was set to 390nm.

2.4 results

The results are shown in FIG. 2, with no significant difference between MYH6 780-WT and MYH 6-E526K (p > 0.05).

3. Myofilament assembly experiment

3.1 cell lines

C2C12 cell line

3.2 construction of adenovirus vectors

The full-length human MYH6 cDNA and its mutant p.E526K were amplified from IMAGE clone 14406689 (GenBank Accession number BC 132667.1) and cloned into the adenovirus vector GV138 (Genechem), respectively, with a 3Flag tag at the AgeI/NheI site. HEK293 cells were co-transfected with either the Ad-MYH6 WT or Ad-MYH6 p.E526K constructs and the packaging construct to generate adenoviruses.

3.3 transfection and visualization of cell morphology

Mouse C2C12 myoblasts were cultured in DulbCo Modified EGO Medium (DMEM) and 10% fetal bovine serum (FBS; invitrogen) was added. In the cell infection assay, the above cells were seeded on precoated glass coverslips in 12 well plates in DMEM containing 10% FBS. Cells were infected with AD-MYH6 WT and AD-MYH6 p.E526K, and then transferred into DMEM (containing 1% horse serum) as a differentiation medium to induce differentiation. After infection, the medium was changed daily. The cells were further cultured in differentiation medium for 7 days. On day 8, cells on the coverslips were fixed in 4% paraformaldehyde for 15 minutes, washed extensively with phosphate buffered saline, and finally transferred to slides. The cell morphology was observed using a laser scanning confocal microscope (leica TCS SP 5) at a magnification of 400x1.4.

3.4 results

3.4.1 morphological structures as shown in FIG. 3, they are classified into three categories according to the structural organization:

(A) Class I: normal bands, cells with clear striated myofibrils, align parallel to each other along the long axis of the cell.

(B) And II: mixed bands, cells containing a mixture of striated muscle fibrils and non-striated muscle fibers, wherein the striated muscle fibers are thin and misaligned.

(C) And (3) class III: no bands, cells containing bright fluorescent spots, lacking the characteristic tissue repeat unit characteristic of striated muscle myofibrils.

3.4.2 After wild type MYH6 and mutant E526K MYH6 are over-expressed in C2C12 cells, myofilament structure analysis and counting

Within the mutant group, the number of class III cells was significantly greater than in the wild type group.

From this, it was found that the researchers could administer drugs to the culture system when transfecting cells, and the therapeutic effect of the drugs on ASD could be judged from the morphological distribution of the cultured cell structure tissue.

It should be understood that the above-described embodiments are merely exemplary of the present invention, and are not intended to limit the present invention, and that any modification, equivalent replacement, or improvement made without departing from the spirit and principle of the present invention shall fall within the protection scope of the present invention.

A sequence table:

<110> institute of cardiovascular disease of Guangdong province

Application of <120> MYH6 gene in congenital heart disease diagnosis product

<160>5

<210>1

<211>26288

<212>DNA

<213> Artificial sequence

<220>

<223> -MYH6 Gene

<400>1

1 agatagagag actcctgcgg cccaggtaag aggaggtttg gggtgggatg ccctgcagcc

61 cgtccacaga gcccccaccg tgagggacct ccttcaccag gagtggggtg caggtcagtt

121 ggaggcctaa gggctctatt aaaactgcct atctccaggc ccagggaagt tccccctgac

181 acaaggaggt tccacaggaa acccagaaac ctcttttctc cttctctgac tctccatttc

241 tttctctgca tcattctgag tctcctacat gttgtctcca tctttccatc ttccttcctc

301 ctttggatgg cttccttccc ttgatcctgg ttttatcttg cctcttggtc ttcatcgaca

361 cttgtcacaa tcatgcttct ttgtctctct cccttgtcct tccttcttgg cacgtgttct

421 cacctccctg cctctctgct tctaaccctg tttccacacc ccgtccctcg cactcatatt

481 gactcggtgc cctttctttt ctgcctctgc gtctttccct ttctgactcc ctggtctgtc

541 ctgcctgtct gcgctctggg gctgcctcca tccccgggtg gcctgcctct gttgttcttc

601 actctcctca tctgttcttc tctctgcccg gctctacctc tgttgttcct tgctccaccc

661 acggtccaga ttcttcagga ttctccgtga agggataacc aggtgagaac tgcccccatt

721 ttctctgcag agactggggc atgcttctcc tgggagccgg attgctggac caggggtctg

781 ctgtcccaag cactcagcgc caacccttag catactccag ccaatgccac cccagggaaa

841 ccccttacag agattgtcct tcagcatcac ctcagagggc aggagaagca gagccctgag

901 taggggaggg tgcaacagca ggtgcctctc ccagggtgga ggagaggagc gggggtaggg

961 aggggggctg cagaggacaa agccactcgc tggagcctgg gctccctcag gagtaacata

1021 gccctcctgt ctctgaccca ggggaagcac caagatgacc gatgcccaga tggctgactt

1081 tggggcagcg gcccagtacc tccgcaagtc agagaaggag cgtctagagg cccagacccg

1141 gccctttgac attcgcactg agtgcttcgt gcccgatgac aaggaagagt ttgtcaaagc

1201 caagattttg tcccgggagg gaggcaaggt cattgctgaa accgagaatg ggaaggtgag

1261 tagggcatgg cgccggggca gaagggaagg aggtctggga aagaagatgc agaggtggag

1321 ccacttgcag ggggagctga gagggctgga gaaaagccaa ggccagtggg gatgccagga

1381 catgctcctt tgaggagccc agaatctgat ccctctcaaa ttgacctgag ctggtgcaac

1441 aggtgccacc cagggccatg ttccccctgc cagagaggat gctgaggaag aagaacctca

1501 gtgttcgcct aagaggggtc ttgtagataa agagggcaca gacacagcat taaatgatgc

1561 ccccttcttg cacttg/atatc cctcttccct gtgcctcagt ttcctccatg agtccacttt

1621 ctcaaattcc gttcacccaa atcaagagta attcttagac ccagatgaac acaaagatca

1681 gaaacttttg agctgagcac tctccttgac tggcactcag aagctctggt ccctggtttg

1741 ctcacaccag ccaagagaat cacccctggt taccagctgc ggctcagggc tgtgtgcctc

1801 atgaactcgt tgactgaatg ttacaaccca ttgaagtgta gaataacagg ccacaatccc

1861 ctggggcttt tgactctgat cccagctctg ccacccgcta gtcactgtgc aggcaaatca

1921 tttagtcatt tagagccacg gatttctcca ctataaaaaa cactggaata cctactggca

1981 ggatctaatg acatcagggc atggcaaact gactgctgcc aatcaaacca caccaacagt

2041 gatggatggg gagtgtggag tagatgggtg aactactttt ccagcagggg tgaaggtttg

2101 ccctgagcaa cagataccct aaagcgctgc ccgcgggaga cagcctcggg gtcagcataa

2161 ggtgtgcaca gatctgagag ctgccaatct ccaggtctgc cccaagaccc ttggaacata

2221 gggactgaag agtgatggtc atgggcacag ggtgtcccca ggatggtctg gggatctggc

2281 aagagaaagg taccctagga cagtctctag gatgggagat acaatgggaa gggaaattac

2341 ctggggaaag tgtcccaggg gacatcgggg taggggccgg ggcactggtc agagcaaggg

2401 gagcaaggcc aagtccctgt gtcctgggag gaggtcagtg ggcagtgctg gcaagggtcc

2461 cggagggatt gtggtcactc atcctcctgc ttatgcgccc cctccagacg gtgactgtga

2521 aggaggacca ggtgttgcag cagaacccac ccaagttcga caagattgag gacatggcca

2581 tgctgacctt cctgcacgag cccgcggtgc ttttcaacct caaggagcgc tacgcggcct

2641 ggatgatata tgtgagtggc tcctgcacac tgcagaggct tcctgtgctg cgtggaggcc

2701 taaataagcc aggggggctc tcccaagaag agggggagag actcccaagg gacccaagtc

2761 cccttccccc ctccacccct agtcagctgc aggaggagta gagccagctg gagtgaacag

2821 ggacatgcct ggctgccacc actgcctgtc ccaggctctc cccaccaacc tcatgcccag

2881 ccttgtctcc tgctccagac ctactcgggc ctcttctgtg tcactgtcaa cccctacaag

2941 tggctgccgg tgtacaatgc cgaggtggtg gccgcctacc ggggcaagaa gaggagtgag

3001 gccccgcccc acatcttctc catctccgac aacgcctatc agtacatgct gacaggtgag

3061 cctggtggcc cctggtctct gctcctcctc ccagacaccc acccagatcc tcagccctga

3121 ccccattgct tctcctcttt tttcttccag atcgggagaa ccagtccatc ctcatcacgt

3181 gagcgagtgc catcctccca cagaagggac tgggctgggg gcatacacgc tgatgcctag

3241 ggtgtagttg ggaggagagg tttaaggctg ggattgcagg gagcatgggg cactgagctc

3301 tcattagagg ggtgccagag caatgaacca tgtcaggcaa atcctgctgg gtgctgggct

3361 ctgatggcca ggctgggaag gggagcatgt gatgctgagc cctgtatgga gaacagtagg

3421 aatcctgagt tttgattggt ctctgtggcc cccaggggag aatccggggc ggggaagact

3481 gtgaacacca agcgtgtcat ccagtacttt gccagcattg cagccatagg tgaccgtggc

3541 aagaaggaca atgccaatgc gaacaaggtg ccatggggga cacaggctcg gcagaacagg

3601 ggttgggggg caggctgacc cgagttaccc ctaaccctcc cctccctgtg acgtggtggg

3661 gacagccaca ctgagctggg ctcccgatgg tcagcccagt atggaagacc ttcctgacag

3721 gagacactca ccctgaggtc tgggtaggat cctgtggagt cgcagaccca ctagcggttc

3781 acccagcccc gcacccccat tcctcagggc cagggcagcc tccctgccct ctcaccactg

3841 cctggaggtg gatggaggat gaacccatgc agttctgctc ctttcatagg gcaccctgga

3901 ggaccagatc atccaggcca accccgctct ggaggccttc ggcaatgcca agactgtccg

3961 gaacgacaac tcctcccgct ttgtgagtgc ctttgaccac tcccagtggc ctcatccagc

4021 cttgacaaga aaaagggggt gctgttttgc cacacccagt tgattgtact gtatctggct

4081 ttgggatatc aacatgcaca cgttggtggg aagagagcat aggctttgga gccaaaggtc

4141 tgactttcca cttgctcagc aagtccttac acctctctga gccctagttc cttcacctgt

4201 gaaacagggg gactaatatc caccctgcag attactggga ggattaggtg agatcatatg

4261 agaggtatga gcaggaggca ttctgtatgt gttcccctct gggccgaggt tccactagga

4321 ggtccctgca cagtgtgtga gggtggcatc ctctgcccgg cttcacttat actcaactga

4381 gacaccaaag aggtcgttta catttccaga accatccagg gcttctgggc tgaacagagg

4441 gccaggattt cactctgtcc ttcccgccag ttccctcact ctgtcccatt cgtccccagg

4501 ggaaattcat taggatccac tttggggcca ctggaaagct ggcttctgca gacatagaga

4561 cctgtgagta ccagcaggga ggctgccacc ctctaggtcc ccctctgcct tctctccctg

4621 cctggcggcc agtctcatca ttcctgccat ctcctgtttt gtctgcattt gcctctgggc

4681 tttaggatcc tttttattta agccacatct tctcgcctgg ccctcttcat caagctgtgg

4741 gtgccccctg ccctctgccc ccatggccac ctttttctgg ctctactctc tttccttcct

4801 cacctgcctt cctcccctcc acccacctca gacctgctgg agaagtcccg ggtgatcttc

4861 cagctgaaag ctgagagaaa ctaccacatc ttctaccaga ttctgtccaa caagaagccg

4921 gagttgctgg gtgaacctgc ctgccacccc ccacccactg ctgtccctgc tgcacacccc

4981 aacgactcct gcatgcaggg cagggccctc ctgctcccca ctgtgcccca ccttgctgct

5041 cacttccctt ctccctccac tcactccgcc agctccctct cgcttccctc ccaagaccac

5101 ctgcgtctcc tctcgcctcc ctgtgcagtc acacacctca ctccttctcc acccttctct

5161 ccactcctcc ctcggcttgt ctttgccttc tcctctttat ttgcctcccc atctcccatg

5221 tgtcactccc ctgccaccct ccctcctcct tgttactgga gcccctgcca gggcccctct

5281 tctacccttt cacttcctct gggttcactc cttggtcctt gctgacttct cttctctccc

5341 tgctccctgt cctcccttcc tccattcacc ccgtccctta cccctccctg cccctccctc

5401 cctttcccaa ctctacctgc cccttccctg ccctccgcct gcccccttgc ccctgcctca

5461 ccccttgcct ggtgcagaca tgctgctggt caccaacaat ccctacgact acgccttcgt

5521 gtctcaggga gaggtgtccg tggcctccat tgatgactcc gaggagctca tggccaccga

5581 tgtgagtgag ggggctgctg cggccgttcg agggacccca ggagctcctg gggccaccgc

5641 tccaaccccc ctctttgtct ctcgctctgt cccagagtgc ctttgacgtg ctgggcttca

5701 cttcagagga gaaagctggc gtctacaagc tgacgggagc catcatgcac tacgggaaca

5761 tgaagttcaa gcagaagcag cgggaggagc aggcggagcc agacggcacc gaaggtggga

5821 ggggcaggca ggcagccctg ggaaggcctg aggtgctgac caggctctct gcgggactca

5881 gatcccagag actctgaggc ttgtggggca gtggggtgtg ttgtgggatg gtgagggaca

5941 gggacgtggg agacgtgggc agacagagag tccaccacaa tcccaactcc tcaaccccag

6001 ctgcactcac cagaactgag gtggggaatt ttcctcctgt gcccaagact cccccagccc

6061 caggaagcac agcccctgag ctctgcacat caccatcact aatgcagggc ctttgcctgc

6121 ccagcccttg ggtaagcccc tcgtgggctc ataccatgtt gtttgggagg cagtgggcca

6181 ggggtcagga catagcccag ggcattggtg tgtcagtttc ctcctcagtg aaatggatca

6241 acaatctttc ctcactcctc tggtggctac tgtgaggtcc tttgagaaaa tgtgaaggtg

6301 caaacagtgc tagacaggtg cccaagaaca ttcctcccta ccttcaggga cccttctcca

6361 agtctgccca gatcaaagtg tcaagctggg gttcatgata acacaccaac agttattaca

6421 acattcttga aattacacca aataactcct cctctaaaca gagaaatacc catgttgggt

6481 ggcacatttg gatggctggg ggtaggagcg tggtactgtc tgatatactc tcagccagtc

6541 agattgggta acatgtggat ggagaagaca gcatggccag tccagggcta gacactgggg

6601 tagagaacga aggagacagg gagcagagga catctagccc ctcagccaca cccttgccct

6661 ggagaggcga gggatatggt gaaggtgggg atatgcccac agcagtgctt gtgaaagacc

6721 tgacccagag atgccacaga tgtagagaca gagctgagaa gagccagttg tggcaagagg

6781 ccatgggccg gataaggggg ctgtgctcca gcctgactca ggcacacgtt ggttgagtaa

6841 ccaaggcaag ccctgtgctc ttccaggcct tgatttcttc ctctttaagg aaaagaaatt

6901 ggacctgatg cttcctaaag tcttggcccc aggacaggga aggaggctca ctccctgaaa

6961 gtgttcactc aggccaggtg cagtggctca tgcctgtaat cccagcactt tgggaggcca

7021 aggcaggtgg atcacttgag gtaaggagtt tgagaccagc ctgggcaaca tggtgaaacc

7081 ctgtctctac tacagataca aaaattagcc tggcatggtg gctcatgtct gtagtcccag

7141 ctacttggga ggctgaggca ggaaaatcac ttgaacccag gaggtggagg ctgtagtgag

7201 ccaagatcgt gccactgcac tcaagcctgg gtgacagagc aagcctctat cttaaaaaaa

7261 aaaaaaggaa gtgctcactt atcctttccc tctcaaccag atgctgacaa gtcggcctac

7321 ctcatggggc tgaactcagc tgacctgctc aaggggctgt gccaccctcg ggtgaaagtg

7381 ggcaacgagt atgtcaccaa ggggcagagc gtgcagcagg tgtactactc catcggggct

7441 ctggccaagg cagtgtatga gaagatgttc aactggatgg tgacgcgcat caacgccacc

7501 ctggagacca agcagccacg ccagtacttc ataggagtcc tggacatcgc tggcttcgag

7561 atcttcgacg tgagttggga cccctgggag tgggagaaca atcactcgct tgctcctaca

7621 ttcaacagcc atttgctgag agccagctgt ggaccagaca tgggaaggca gtggggactg

7681 tgtggtgaca gaggcagtca ttgtccctgt cttcagggga agccctcctc cactgccctg

7741 acatggaggg gacagccata ccctgctggg ctcggcacag tgcacgggca cagccccaat

7801 ggccactcac acccactttc tgactgctcc cacccctcat gccccctgca gttcaacagc

7861 tttgagcagc tctgcatcaa cttcaccaac gagaagctgc agcagttctt caaccaccac

7921 atgttcgtgc tggagcagga ggagtacaag aaggagggca ttgagtggac attcattgac

7981 tttggcatgg acctgcaggc ctgcattgac ctcatcgaga aggtgcctcc ttggcctcac

8041 cacctatgcc ccctcctctg ccatccagac aaagtggtgg ctgagtccct tctacaccca

8101 agaaactaga gtcccaagaa tcccaggcct ttctccaggc ccagcttctc cccactgtga

8161 agtcatgggc atgaacagga tgatcccccc actcttcctt tcccaggacc ttgcacttta

8221 tgcccctttg tggtggtccc ctcagtgtct taagagtgag atgtagtgaa ggagaggccc

8281 ctggcccctc tgaccgccca tgagaagcgt cattcatgga aagatcctag gctgaaatta

8341 gagatgtttg gcctcccacc accttcctgt tggttgagaa ataagccagt ctccagccct

8401 cttgcttatg ggcattcctc agaagagaca aggccgcagg cgggaggccc cataggctgg

8461 ggctgacttg ctctcagtga acctctgctc tttgtcagca taggccagag cccggattgt

8521 ctgacccaca cccagcccag ccacggcctt catgaaatgg gagcttcccc acatgctttg

8581 ggtcattatc cagattctta accagagttc tcatgtttca gagccctcag aatgccataa

8641 aactgtgtgt ggaaaaaagc atgtgtacat tcatacatac atgtgtgtgc ttgtgcatgt

8701 gtgtgtgtgt acgtgtgtgt gtgtacatgc gtttttctgg agagacagtc tttagcttca

8761 acaaattctt aaaaggattc ctgccccaag aaaaatcaag aaccaccagg tttttggaag

8821 tgcagagtgt ggtaaagaac ctggatcctt ctatctaacc cttgaagaat tgctactggt

8881 gtcatacaga cagagcctgg cccggtgcct ctgacacaca tctgtccctg ccctcctgcc

8941 cctggggcag tgagccatca gagagaaggg gctgtgctgt gaggcactgt gggccttgtg

9001 gggggtgatt aggagtgcat gactctttgt tggacccact gggcagaaag gaggaggaag

9061 gggcttttta aattccagga atattggctt cctgttttag ggtaagaggt accagcacag

9121 cgccccttca gcaggccagc gctactggct ccagattcct tttcctgtca gggtatggga

9181 ctgtggaagc ctgggagtgt gctcagtgat tctctctttg cctcttcacc ctgccctcag

9241 cccatgggca tcatgtccat cctggaggag gagtgcatgt tccccaaggc cactgacatg

9301 accttcaagg ccaagctgta cgacaaccac ctgggcaagt ccaacaattt ccagaagcca

9361 cgcaacatca aggggaagca ggaagcccac ttctccctga tccactacgc cggcactgtg

9421 gactacaaca tcctgggctg gctggaaaaa aacaaggatc ctctcaacga gactgttgtg

9481 gccctgtacc agaagtcctc cctcaagctc atggccactc tcttctcctc ctacgcaact

9541 gccgatactg gtaagcaggc agcccctgca ctgggccagg ggacttctga agacacaaag

9601 ggccagggtc ctgctgcttc aaagcacatg actccatagg caggcacagg ggagcccatg

9661 agtcaagcca cctcctggct cacctcacca cagtccagct gggcttctgt agcacctgcc

9721 gttctccact ttgtgttatg agtgttgata tagctgtctc atgtttcctc aagatgggga

9781 gtaactggag gtcagtgagg gtggcacatg catccctgta ccatgcccag cagggaacga

9841 tgcatgtggg agttctcaac aaatgttgag aggacactga acaaaagagc ctccagttca

9901 gaaaaggaag agaaaggatg tggcttgaaa tgaagaggat aaagtgtaag aagaaaagta

9961 gaggttgggc acggtggctc acgcctgtaa tcccagcact ttgggaggcc gaggcgggtg

10021 gatcacaagg tcaggagttc gagaccagtc tggtcaacat ggtgaaaccc cgtctctact

10081 aaaaatacaa aaattagcct ggcgtggtgg cacctgcctg tagtcccagc cacttgggag

10141 gctgaggcag gaaagttgct tgaatccggg cgacggaggt tgcagtgagc caagattgca

10201 ccactgcact ccagcatggg tgacagagtg agattccatc tcaaaaatag aaggaagaag

10261 aagaagaaga agaagaagaa gaagaagaag aagaagaaga agaagaagaa gaagaagaag

10321 aagaagaaga agaagaagaa gaagaggagg aggaggagga ggaggagaac tagaatgtgg

10381 aaagatataa gaaggaaggg caagtagagg gagagctaag ggcactgatg tatgtcagtg

10441 cctactatgg gcaagcacca gtgagctgcc ctacaggcgt ggtcttatct aatcctcttt

10501 acaactttgg gaagttgtta ctgtcatcct catggtaaac tgaatcttgg agaattcctt

10561 gcccaaagtc tcagagctac caagcgagca gcagagctct gcctctgccc aagggctcct

10621 ttattttcca gctcctgttg attatttctc ctctcgctgt ttaggggaca gtggtaaaag

10681 caaaggaggc aagaaaaagg gctcatcctt ccagacggtg tcggctctcc accgggtaag

10741 aagggcccag gggtgccagg acacctggtg gaatggccca gcccagagac ttctggctgc

10801 accacctatt ctgatgcttg agtttgatga ggaaagaagc tagggctacg tagtcgtttt

10861 ttagtgtgta gattccacaa aagcctgaac tcacgtcaca aattatgtgg cagcccctgt

10921 ccctttgatg attctggtgg agagtgtctg gggaccaggg tttaagggcc taagggatgg

10981 tccttgtggc tcctaactcc ccaccttcat ctgcctccag gaaaatctca acaagctaat

11041 gaccaacctg aggaccaccc atcctcactt tgtgcgttgc atcatcccca atgagcggaa

11101 ggctccaggt gagccaggag aagaccttag tctggggagg acagctggca tccactttac

11161 cctaaggctg accctttccc cttccctcct gacacagggg tgatggacaa ccccctggtc

11221 atgcaccagc tgcgctgcaa tggcgtgctg gagggcatcc gcatctgcag gaagggcttc

11281 cccaaccgca tcctctacgg ggacttccgg cagaggtggg tatgagggtg ccccagagct

11341 catagaacag ggggagccag gctgccctga tgggaatggg atctgcaggt gaccctggaa

11401 ttctgtgggc agagcagatc actgcagagc atgggtgact ctggacactt ccctcctcag

11461 gtatcgcatc ctgaacccag tggccatccc tgagggacag ttcattgata gcaggaaggg

11521 gacagagaag ctgctcagct ctctggacat tgatcacaac cagtacaagt ttggccacac

11581 caaggtgagt ctagagcccc actgggtggt tgcagggcag gtggccatgt tgagtggagc

11641 agagaggagt ttaggaggca gaagcctaat tctggcttcc ttatcaacct tatcaagggc

11701 tgaaacccag gcttcattcc ggtcttgttt gtcaaatttt tactcttact tctagaaggc

11761 atggggtgat gggtcacctg ggagctcatc cagggtcttc caccctggat actcccctct

11821 gaggctgcgg cctgttgcat ctaccccttg cctgcaggtg ttcttcaagg cagggctgct

11881 tgggctgctg gaggagatgc gggatgagag gctgagccgc atcatcacgc gcatgcaggc

11941 ccaagcccgg ggccagctca tgcgcattga gttcaagaag atagtggaac gcaggtgaga

12001 caggaggaaa agggaggcat gcactagaga tgtagaggca gatccgcaat gtcaactagt

12061 gtgggtcaga agacctgggt tcagacctac cagctggcct gggcaagtta ctttaccact

12121 tcgaatctca ttgcaaattc ttcttcttct tcttcttctt cttttttttt ttttaaagaa

12181 acttctctat aggaaaatgc aaattaagac accaatgaaa taccatttta aaacactaga

12241 ctttgcttgc tcagatggtc tacaaatttt aaaaaatgaa agaaacaagt agataaaaat

12301 aaaatgaaag gaaaaataat ttttaaaagt tttaaaaatc actagactga caaaaattaa

12361 gaagcctgac ccactgctta cagatgtgta aattagtgca acttcctagg aaaacagttt

12421 atcactatct tttaaagttg aacagctgca ggccaggcgc ggtggctcat gcctgaaatc

12481 ccagcacttt gggaggccga ggcgggtgga tcacgaggtc aggagatcaa aaccatcctg

12541 gctaacatgg tgaaacccca tctctactaa aaaaatacaa aaaattagcc gggtgtggtg

12601 gcagatgcct gtagtcccag ctactctgga ggctgaggca ggagaatggc atgagcccgg

12661 gaggcagagc ttgcagtgag ccaagatcga gccactgcac tctagcctgg gcgacagagc

12721 gagactccat ctcaaaaaaa aaaaagttga acagctgcat acttctccat tccactcctg

12781 agtacattcc tacagagaaa ctcttataca tgtacaccat gacatgaaaa ataatctttt

12841 agcagcattg gtcataatag caaagatctt aaaacaactt gaatttccat ccacagggga

12901 atgggtaaat agtattacag tcattcagtg gaatattata aaacagtgaa aagatgtgaa

12961 ctccaactac atgatacaat atgtttgaac attaaagcat attgttagta aacaaaggca

13021 aatcttggaa ggatacatac agtatgatgc aatttttata aagctcataa ataatcaaaa

13081 ccagccattc attgtttaaa gatacataca tatggctggg cacagtggct tatgcctgta

13141 accccagcac tttgggaggc taaggcaggc ggatcacctg aggtcaggag atggagacca

13201 gcctggccaa catggtgaaa ccccgtctct actaaaacta caaaaattag ctgggcatgg

13261 tggcaggcac ctgtaattcc agctactcag gagctgaggc aggagaatcg cttgaatccg

13321 ggtggcagag gttgcagtga gccgagattg tgccattgca ctccagcctg ggcaacaaga

13381 gtgaaactcc atctcaaaat aaataaagta aaataaaaat aaagatacat tcatatgtgg

13441 ttaaagtaat ttttgaaaaa gcaaggaaag gaaaaataag attcagagtg gcggctgctc

13501 ctggcggggt gacaggggtg taggaaggaa cgcacaggta gatcaatggc tggtaacatt

13561 tcagctcctg tttcagtggt aggttcatgg atagtcattt tgctttcatg ctgcataact

13621 tacatactta ttatagacat tcttatatgt aaacccctac ataattttta acatatttga

13681 agttcttatg agaatagaaa aaccaaacag agtagacatt ctataagtgg tagtttccat

13741 taccactaat atcccagctg ggaggatata aactgccctt tcatcaaatt gattaataaa

13801 tatttatcaa gcaactccat gttcagtgta gtgatgttgg aggtgggtgt gcacaagaag

13861 gaagtctacg tgcctacgaa cttgcttagt agggcccatg attgggaagc tctcttttat

13921 agtgccccac cctgcctcca cgtttccttg ccagggatgc cctgctggta atccagtgga

13981 acattcgggc cttcatgggg gtcaagaatt ggccctggat gaagctctac ttcaagatca

14041 agccgctgct gaagagcgca gagacggaga aggagatggc caccatgaag gaagagttcg

14101 ggcgcatcaa agagacgctg gagaagtccg aggctcgccg caaggagctg gaggagaaga

14161 tggtgtccct gctgcaggag aagaatgacc tgcagctcca agtgcaggcg gtgaggccac

14221 gtgattatct cttcagccct ctcctcccct cccctagatt atagcccatc tcacaaccag

14281 ggactgggag tctaggagtg ccagctcttt ttaagaccct aggtctcctc tctttaacca

14341 tcagcttcct ccctgttctc ttctcccagg aacaagacaa cctcaatgat gctgaggagc

14401 gctgcgacca gctgatcaaa aacaagattc agctggaggc caaagtaaag gagatgaatg

14461 agaggctgga ggatgaggag gagatgaacg cggagctcac tgccaagaag cgcaagctgg

14521 aagacgagtg ctcagagctc aagaaggaca ttgatgacct ggagctgaca ctggccaagg

14581 tggagaagga gaagcatgca acagagaaca aggtgagggc agctccctct ggcttcagcc

14641 caggtctcct caagactccc agactagagt gttgtcctgg tccttggcat ggaggtcccc

14701 atagatgtct ccaggctggt gatctttgac cctaaagggg atgggttttt ggtcggcagg

14761 tgaagaacct aacagaggag atggctgggc tggatgaaat catcgctaag ctgaccaagg

14821 agaagaaagc tctacaagag gcccatcagc aggccctgga tgaccttcag gttgaggaag

14881 acaaggtcaa cagcctgtcc aagtctaagg tcaagctgga gcagcaggtg gatgatgtga

14941 gtagtaagaa ccatgctcct gctctcagag caagattttg caggcaacac caatggccca

15001 gaaagtcctg atccctagaa ttaacttcta tggcccctga agcttttttg ctctctgtag

15061 ttcctcacta cagtaggtct ctgaatcctt tgtgcttgca ggatttctct gttggtttga

15121 cttccaatcc cactggactt caagtttaga aggaggcaaa agagcataca ctatggattt

15181 catgttttcc acactttgct tattttcttc cctccaacag ctggagggat ccctagagca

15241 agagaagaag gtgcgcatgg acctggagcg agcaaagcgg aaactggagg gcgacctgaa

15301 gctgacccag gagagcatca tggacctgga aaatgataaa ctgcagctgg aagaaaagct

15361 taagaagtag gagactgtgg tggccaggag gggctaatgg aggtgtctgg cctggtagat

15421 agagtgcagg gtgctgcgcc tccactggcc acagttgcct tgggtatgct gggaataagg

15481 tcaatcacag ccctcttccg ccactttctg ggctggcgag gggaggggga ggtgccgatt

15541 ctggcatact aaggccaaga agagaatgag ccccagagga aggaaggcta cctgtcactc

15601 ccccaccccc acccttctcc tgcaggaagg agtttgacat taatcagcag aacagtaaga

15661 ttgaggatga gcaggtgctg gcccttcaac tacagaagaa actgaaggaa aaccaggtga

15721 ctttttttcc cagtgcatga aagtgggagc tcaatagccc tgaggtaact gaggctgcag

15781 cagctgctta gggttctaca caatatctgg aactccaggc agcctcatag acccaaccat

15841 ccctgactta caggcgctca ggaacactag ccttccccca tagagcaaga atacattacg

15901 ttagcaaaac tgtttgagaa gggggactca tacctactaa agggcttctc cttctgagat

15961 ctgctctgcc tcagggactt tctactctaa gacaaatgat agagctctat tctcctctaa

16021 cccttcctct ggtagttcag ccctctgaac gcagggtgaa aaaaaaaaaa ggcttcaagg

16081 agcaccttcc tgtgagctgc tctgttcata ttcttcccgg tactaacccc agccactaac

16141 ccaggctgag tccccatagg gcactcatga taagagccaa ttccaacaac tgtgagcaag

16201 tcacttaccc tccctgatcc tcagattctc ttctcacctg taaaatgaga ctgacaatac

16261 ctaccacaca ggaccagagg ggataatgta tttgaaacca ttttgtcaac taggaaattc

16321 tatacaagta tcatattaat atagcccaat ttagagaagt cagccagcca ctagcctaag

16381 aaggtattgt tttgtcttct caatggcctt ctcattcctc tgcagctcaa gatttagtga

16441 acagccatag tctcccttag ctttaatgaa cctcaaaccc taatgtgctt ttagtatcgg

16501 gtttgtcaga agagacctgg aagtccactg ccatccccat tatagagcta cagaagctac

16561 atggccaggt ctggacatag tgaccccaag ggcaacagga actcggctac tggggcggga

16621 ccttgtcctc tcactttggg gcagactgtg gctggtagaa agaggtagtc tcccctctgc

16681 acttgaggcc catggcccag gctgcaagta atgtatgaac acaattccac tcctctgggg

16741 ctgcacaggg actggcccgc cttcattagt aatttgccct cccatcttct tggatgcccc

16801 ttctgggttt tagctagaat atcgggccat cctcaggttc ttctacccta ggtctgggca

16861 acttgtttgg cctcttggac acagaataac agtcctgtta gtctcatcag ctctcggcaa

16921 taggctatgg ctcctccttc atccccaaga tcttgctcag aagtcccacc ttctccatcc

16981 tgggagcaga ggcatggtgg tcctctctgc tgcttgtagg acccatccat gaacagtcca

17041 ttaaagctgt ccataacccg aggtgaaggg atttccactg aacccccgtt ttgctcctgg

17101 ctagccagac aacagatcaa aatggtagat acgatattct cccaatttag tcaagaccaa

17161 ggctaaggcc caaaaaatga ggtaagggca cttaaagagg ataaggagat gaggtgaaga

17221 ggagagtagc ctgctagcac tttcctggcc tggaagggag caatcatgtt gaaacccagc

17281 cctgggcata aacacagcaa gcctgggaga gcaaggaata tggttgattg gactttgtgg

17341 ttaacttgga gaattgcaaa ggtatctgat tgtttcgagg catgttgtca caaatatttg

17401 taaaatacaa gcactcattt tcccgtctta tgaatagcgc aacagagcct agtgaatctg

17461 gggactctga acttcttgat ctcacaggat accaggatcc cccttcaacc acaggttctc

17521 aggatttggg gctgcagatg ctcacactgg gtctgagatg cccttgggag cttcagccaa

17581 attcctattg atggcctatg cattatagga tgtttagtag catccctggc ccctaccctc

17641 tagatgccag tagcaagccc caccaagaca tgacaatcag acattgccaa ctgttccctg

17701 gggcacaaaa ttgcccctag ttgagaacca ctgcttgaga ggaacctaag ttcctggtag

17761 cttttcagag ccggggggat tccagtggag gggtccaggc ggtgggtctg agccctttgt

17821 gtctgaccca ggcacgcatc gaggagctgg aggaggagct ggaggccgag cgcaccgcca

17881 gggctaaggt ggagaagctg cgctcagacc tgtctcggga gctggaggag atcagcgagc

17941 ggctggaaga ggccggcggg gccacgtccg tgcagatcga gatgaacaag aagcgcgagg

18001 ccgagttcca gaagatgcgg cgggacctgg aggaggccac gctgcagcac gaggccactg

18061 ccgcggccct gcgcaagaag cacgccgaca gcgtggccga gctgggcgag cagatcgaca

18121 acctgcagcg ggtgaagcag aagctggaga aggagaagag cgagttcaag ctggagctgg

18181 atgacgtcac ctccaacatg gagcagatca tcaaggccaa ggcaggctct gctcggcctc

18241 ccctcgccct ctcccctgca cagcggagcc tcccccatgc cttctctctc tgtctgccat

18301 ctcccttgtc attctcattc tcttcatcac cctttggtct ctcttcctgt ctcccctgcc

18361 cctctctggc tctcctcacc ctctctatct cttcatgttc ctcctttctt taattcaagt

18421 ctctcttcag actgcgccct cccacacctt ctgtgtcccc ctcctgccct ctggcattcc

18481 ccatctctga ccctctcttc cttcctctgg tcgactcagc ccctcccaca ctcacccttc

18541 ctgtcttgct tcctgaaggc aaacctggag aaagtgtctc ggacgctgga ggaccaggcc

18601 aatgagtacc gcgtgaagct agaagaggcc caacgctccc tcaatgattt caccacccag

18661 cgagccaagc tgcagaccga gaatggtggg tgcccctaac caaccccctg cctagggcag

18721 gacatgactt gtgaaatggc ccacaagccc ctcatttcac ctccaggaga gttggcccgg

18781 cagctagagg aaaaggaggc gctaatctcg cagctgaccc gggggaagct ctcttatacc

18841 cagcaaatgg aggacctcaa aaggcagctg gaggaggagg gcaaggtgag gcccagtggg

18901 gagggtgggc aggcttgatg gcagccctgg ggcaattcat ctcagtgcca gaaatggagc

18961 ctggagctgg aaagagtcct ctgcaaggga aagaccctcc agtctaggtt ctgccctgca

19021 gctaagcgtc atttaatgcc tcttttctta ttcgtaaggg gatggggtga gcagactggg

19081 aaactcctca aacagtgagg tgccacatca gcccacatgg tgaataaggc tgggcttggt

19141 tgaagtacta cataagaaga gaatctagag aatggggcac agggagtccc tcccacctcc

19201 tggtgccccc ccccctcccc aggcgaagaa cgccctggcc catgcactgc agtcggcccg

19261 gcatgactgc gacctgctgc gggagcagta cgaggaggag acagaggcca aggccgagct

19321 gcagcgcgtc ctgtccaagg ccaactcgga ggtggcccag tggaggacca agtatgagac

19381 ggacgccatt cagcggactg aggagctcga agaggccaag tgagctccag ataccccctt

19441 aacctgactc tcagagagga aggggcgaga ggacctgggg tggggacagg caaagtggtc

19501 atgagacgga agtggaagag acaggaggaa ctcggagggc aacagaagtg cttggaagaa

19561 agcctgaact ctttgctctg tgaactctgg ctggccctga cccacttcct gtgacgggcg

19621 agcttttggc ccgggttata cctgatgctc acgtataaga cgagcaaaaa gcttgttggt

19681 cagaggagct accgtcgatc agcctgtgtg gggggtgagg gcagggggca ctgacaccca

19741 gatgccactg caggtaggga ggacgcctgg gcagcccgtg ctggcggact ctgttccagg

19801 catgagcagg ctcagctcct gctaggctgg acttacggtg tctcaaggag atatagggag

19861 ggggtggaag gaggtccacc caaggctcca gtgttgccca gtagagtcac acacacaccc

19921 tccaccctca cctgggcaga aagaagctgg cccagcggct gcaggatgcc gaggaggccg

19981 tggaggctgt taatgccaag tgctcctcac tggagaagac caagcaccgg ctacagaatg

20041 agatagagga cttgatggtg gacgtagagc gctccaatgc tgctgctgca gccctggaca

20101 agaagcagag aaactttgac aaggtggacc atgggcgggg gccgcagcca gcatgcaggg

20161 caagggggca tgaggggttc agtgagaggc caaaggcaac ctccttggag gtggaggagg

20221 agggctaagc ccaggctcgg gaccagggac agatcttgga catgcggctg aggctggggg

20281 ctggggcact gggaagcagg agggctgggg agctaaggct ggggggctga agagtgagcc

20341 ttgtccccgg gcagatcctg gccgagtgga agcagaagta tgaggagtcg cagtctgagc

20401 tggagtcctc acagaaggag gctcgctccc tcagcacaga gctcttcaag ctcaagaacg

20461 cctacgagga gtccctggag cacctagaga ccttcaagcg ggagaacaag aaccttcagg

20521 gtgtgctggg ggtccaagag gccagagatg agttggtggg agggagggcc atgcaggggc

20581 agggggaaca taggctttga gctttctggc cctctggtcc ccagaggaaa tctcggacct

20641 tactgagcag ctaggagaag gaggaaagaa tgtgcatgag ctggagaagg tccgcaaaca

20701 gctggaggtg gagaagctgg agctgcagtc agccctggag gaggcagagg tgagggccga

20761 gaactccctg caccccatcc ctgttctgcc gctgtctccc acttctccct cacctggggg

20821 tgaccctgac cccaggacaa aatctattca ttccatattc tttgttcaac attatctacc

20881 cactattcac tacccatgac tgcctccaaa gccaaggtct gcacaaagga aaatgtcagt

20941 ctgattagag gccaaaggtt ttgtttccca ccaggtttta aaagttggaa tctggccata

21001 ataataacca cttagacagt gggtaggagt gtggggttcc agagtcaaac aggcctgagt

21061 tggaacacag cttgaggact ttcactgcag cgttgggcaa aatctttaat ctctctctgc

21121 ctcagttttc tcatctgtaa aatggaatta actgtacata cctcatatga ttttacacat

21181 gattattata aagattaaat gaggtaacac atttaagcac ttaatatata ttagctattg

21241 ttactcttca tttctaagca cctgggaata gccctgagtt ttctgggctt ctaacttttc

21301 tcaggaaagg gtgagggacc tggaaaacaa gataaaattt tgtatttgtg gttataaaaa

21361 ttcctccatt ttctataata ctttagacag gtcaggaatt tatgcagtgt tagtgctgga

21421 gggtctggca attgtgaggt ccaaaccctt ttcttgacag gggaagccca gagagtccag

21481 cactttctcc ccaatagcac cgtggtgggc atggggtgca gatcccacct ccccatctct

21541 tctcagctct tcttctctgg gcgatagtcc tggctgacac cgtgtatctt ctcatcctcc

21601 ctctcaaccc tgccctgtgc cctgtctgcc cgccctcgcc ccaccccttc ccaggcctcc

21661 ctggagcacg aggagggcaa gatcctccgg gcccagctag agttcaacca gatcaaggca

21721 gagatcgagc ggaagctggc agagaaggac gaggagatgg aacaggccaa gcgcaaccac

21781 cagcgggtgg tggactcgct gcagacctcc ctggatgcag agacacgcag ccgcaacgag

21841 gtcctgaggg tgaagaagaa gatggaagga gacctcaatg agatggagat ccagctcagc

21901 cacgccaacc gcatggctgc cgaggcccag aagcaagtca agagcctcca gagcttgctg

21961 aaggtacatg ggggcgggag gtcccctcag gggactggcc tccatgtggc ctggagaagc

22021 agtggtgtct ggatacaggc accagattcc tcctgcccct gggttactgc agggacctct

22081 gacaggtgcc ctcagtgaag ggcaccgagg ctggcttctg ctcacaccca ctctcctgat

22141 gctcaggaca cccagatcca gctggacgat gcggtccgtg ccaacgacga cctgaaggag

22201 aacatcgcca tcgtggagcg gcgcaacaac ctgctgcagg ctgagctgga ggagctgcgt

22261 gccgtggtgg agcagacaga gcggtcccgg aagctggcgg agcaggagct gattgagacc

22321 agcgagcggg tgcagctgct gcattcccag gtgagggggt caggagccac cttgtggaaa

22381 cctactgagt gcagagccca ggacatctag aaaagccaga tgttctaagt gagcacatct

22441 agccagggtc acaaatcatt tcctctctta ggccaactct catctgtggt ggctgcagaa

22501 ccattatatt aagaaagcgg tctctactta gaagctaaga gggtcatgat tgattgctga

22561 tacctgccaa agccacaaat ctgggagtag tggaatgtat cctgttattt gatattcttg

22621 atctgaaaga cagcctccgg cttgctctgt aaagatgaga gtttgggagt ttaaagaagc

22681 ataactgcat ctcttgcaaa ccaagctggc tagaacacca ttttctgcaa tcccacccac

22741 actgttttca gttatactct gcaatgagac tttgtgctca ccttttgcag tgccctgtgg

22801 agttttgttc tttaatctag tatgtgtgag aatgacatca tccacttacc tcatcccctc

22861 ttccttaccc cactacattc ttggtatagc tataaacatc tctggaatat tgtcctggta

22921 aaaagttgtt tccatcttcc ttggaatatt gtcatgctcc tacgtaaaca tgttgctaaa

22981 gagctccagg gtaatactgc agaccttttg ctatttgaag tcttttttca gagtttagta

23041 atcttagata caatgtctag aatgtatgca ctttcatagg aaaagggaaa gcaggtacaa

23101 aacgatcaat agtctaaaag tgggcttgtt gttttaaaaa aaaaatgacc acctttaatt

23161 ctttctggag aaagggtatg aaatcaggta acaaagtgta gtatatattt gatcattttt

23221 ctctctccat gtctagaaca ccagcctcat caaccagaag aagaagatgg agtcggatct

23281 gacccagctc cagtcggaag tggaggaggc agtgcaggag tgcagaaacg ccgaggagaa

23341 ggccaagaag gccatcacgg atgtaagtga ccgcccacct tccgcctccc ctaaagacag

23401 aaacaaggcc ttgggtccag gccaggccac tgtgctgtaa caccaagcca actctgcagt

23461 tctgtggatt tgagggcctg atgggagaaa ggagatcctt ggggggcaaa aggccccggc

23521 ccctggccca tgttccttgc cacctctctc ctgcacacag gccgccatga tggcagagga

23581 gctgaagaag gagcaggaca ccagcgccca cctggagcgc atgaagaaga acatggagca

23641 gaccattaag gacctgcagc accggctgga cgaggccgag cagatcgccc tcaagggagg

23701 caagaagcag ctgcagaagc tggaagcgcg ggtgcgggag ctggagggtg agctggaggc

23761 cgagcagaag cgcaacgcag agtcggtgaa gggcatgagg aagagcgagc ggcgcatcaa

23821 ggagctcacc taccaggtgc ggcggacgcc agacaccagg agtagatgtg gaagtttctt

23881 ctctggcccc actgccccgc cctcacaggg ctcctctcac ctcctccttg agatgctgtt

23941 ggtagattta acgttcttct cacgctctgc agtcagtttg acttgagtct atgagttttt

24001 ccagcaaatg aagaatctac ttctacttcc tgaaaactct tctaactagt ctttccccag

24061 gtttctttct ttcttttttt ttttttttaa taactctaag tgctaccatg aagacttcag

24121 aacagttcaa agaatccttc caccttcgac tgtggggata agagtcaggg gaggggaaaa

24181 gacccggaaa tcttccatag aacttctggc acacaaagag aaggccacag agaaagagga

24241 ccctagaatg ctctaaaacc tccacttgca tagctgagag ctgtgccctt ggcccgttat

24301 tttcagtgta cctgggaaga aaaggccaag gagacgaggg tgtcagtcca tttgatagat

24361 ggataccaga ggcacaagaa agaggttaca gatacagaac cacagagtga tttgtggaca

24421 gaagtagaaa tggcatcctg gcacatacaa tgataaagag ataggaatga tcgagtgacg

24481 ttggagccag tgatcccgat gcctgaattc tggcccagta caatatatta gaatgtagaa

24541 taatctggat tatgataata cccccttctt tctgcattct ttttctggtc aagaactact

24601 ggccaagaga acctatgtaa gtccaggttg gagctttatc caccatactg gagctggaac

24661 agacctggtg cttttatatt accacattag ggaattccat taggttctga gcccctcccc

24721 ctacttctag ctttatgact tcagccttca ttgctctgtg gatccctgac tgacaacctt

24781 gcattgcccc tttgacctac gatagagtca gagaatcttc cccaccacct ctttgacctg

24841 gatcattgca gggaggggca gcaaaggcaa ggggagaaga gtaaaatgat ggaggaggga

24901 aaggtgattg catttgctcc ccctccaaac cagcttctcc caccctccca cccccagaca

24961 gaggaagaca aaaagaacct gctgcggcta caggacctgg tggacaagct gcaactgaag

25021 gtcaaggcct acaagcgcca ggccgaggag gcggtgagtt cagagctttc ttccctttct

25081 catcaacaca cctactattt gtgagaacca atgaatatct cctacagagg ggcctggaca

25141 aagagtttgc tataaacttt aactctcaaa catttgtttg acacatctgg tatgctcaga

25201 gctgtcaggt gttctgaatt aacaaaggca ccacctacaa gctgcttaca attcagatac

25261 cataacccaa cagaaggcag tgtagatgct agtgcaggac gtggggcagc cgaagctgaa

25321 ggcaggaagg tggcggtgga attgggccta gaaggggacc cagctagcca cagggcagtg

25381 gggaagacat ctgggtggat gtgagttgct gattagcatg cctgcacagg aagctggggt

25441 ccgggacagg tgcagcaaaa caggattctg aaggggccca gatcgggcag catgggattt

25501 gtctggggca gtggatggcc gtgaaggact ctgagtgctg gacatgtttg agaagagtgc

25561 aaggcagttg caggataccc ttgggaaggc tgttgcagga atatgcatga ggtatgggtg

25621 cctcagggac agggagctgg aacctcaggt tgagaggctg agaatcccat agcccatctc

25681 cagctcattc acccatcccc actgtcccac cacaggagga gcaagccaac accaacctgt

25741 ccaagttccg caaggtgcag catgagctgg atgaggcaga ggagcgggcg gacatcgctg

25801 agtcccaggt caacaagctt cgagccaaga gccgtgacat tggtgccaag gtgggtccct

25861 cccctgggct tcactagtca cttccacatt agcatgcccc ctgatatggg tgcccttcag

25921 agtgggcact gcttgcccta tatgtaggca gttctgaggg tcccatagct tacataacct

25981 gagaatccac tctcctgctc aaaacagccc cccactgact ggaacttctg cagagatccc

26041 cagttccatc cccctaaacc acaagtgcct ctaacgtggg accacaggat ccctggggcc

26101 ctgcctctcc ctccaagggc atctccctta ggcctctgaa agccccaggg atttgtcccc

26161 acacacttct ccctcttgcc agctgccccc tcacacctct tattcttttt gcagcaaaaa

26221 atgcacgatg aggagtgaca ctgcctcggg aacctcactc ttgccaacct gtaataaata

26281 tgagtgcc

<210>2

<211>1939

<212>PRT

<213> Artificial sequence

<220>

<223> -MYH6 Gene expression product protein

<400>2

1 MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEFVKAK ILSREGGKVI

61 AETENGKTVT VKEDQVLQQN PPKFDKIEDM AMLTFLHEPA VLFNLKERYA AWMIYTYSGL

121 FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE NQSILITGES

181 GAGKTVNTKR VIQYFASIAA IGDRGKKDNA NANKGTLEDQ IIQANPALEA FGNAKTVRND

241 NSSRFGKFIR IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD

301 MLLVTNNPYD YAFVSQGEVS VASIDDSEEL MATDSAFDVL GFTSEEKAGV YKLTGAIMHY

361 GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL CHPRVKVGNE YVTKGQSVQQ

421 VYYSIGALAK AVYEKMFNWM VTRINATLET KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN

481 FTNEKLQQFF NHHMFVLEQE EYKKEGIEWT FIDFGMDLQA CIDLIE/KKPMG IMSILEEECM

541 FPKATDMTFK AKLYDNHLGK SNNFQKPRNI KGKQEAHFSL IHYAGTVDYN ILGWLEKNKD

601 PLNETVVALY QKSSLKLMAT LFSSYATADT GDSGKSKGGK KKGSSFQTVS ALHRENLNKL

661 MTNLRTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV LEGIRICRKG FPNRILYGDF

721 RQRYRILNPV AIPEGQFIDS RKGTEKLLSS LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD

781 ERLSRIITRM QAQARGQLMR IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP

841 LLKSAETEKE MATMKEEFGR IKETLEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL

901 NDAEERCDQL IKNKIQLEAK VKEMNERLED EEEMNAELTA KKRKLEDECS ELKKDIDDLE

961 LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA LQEAHQQALD DLQVEEDKVN

1021 SLSKSKVKLE QQVDDLEGSL EQEKKVRMDL ERAKRKLEGD LKLTQESIMD LENDKLQLEE

1081 KLKKKEFDIN QQNSKIEDEQ VLALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD

1141 LSRELEEISE RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD

1201 SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV SRTLEDQANE

1261 YRVKLEEAQR SLNDFTTQRA KLQTENGELA RQLEEKEALI SQLTRGKLSY TQQMEDLKRQ

1321 LEEEGKAKNA LAHALQSARH DCDLLREQYE EETEAKAELQ RVLSKANSEV AQWRTKYETD

1381 AIQRTEELEE AKKKLAQRLQ DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA

1441 AAALDKKQRN FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF

1501 KRENKNLQEE ISDLTEQLGE GGKNVHELEK VRKQLEVEKL ELQSALEEAE ASLEHEEGKI

1561 LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHQRVVDSLQ TSLDAETRSR NEVLRVKKKM

1621 EGDLNEMEIQ LSHANRMAAE AQKQVKSLQS LLKDTQIQLD DAVRANDDLK ENIAIVERRN

1681 NLLQAELEEL RAVVEQTERS RKLAEQELIE TSERVQLLHS QNTSLINQKK KMESDLTQLQ

1741 SEVEEAVQEC RNAEEKAKKA ITDAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD

1801 EAEQIALKGG KKQLQKLEAR VRELEGELEA EQKRNAESVK GMRKSERRIK ELTYQTEEDK

1861 KNLLRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK VQHELDEAEE RADIAESQVN

1921 KLRAKSRDIG AKQKMHDEE

<210>3

<211>20

<212>DNA

<213> Artificial sequence

<220>

<223> detection Probe

<400>3

cttctcgatgaggtcaatgc

<210>4

<211>20

<212>DNA

<213> Artificial sequence

<220>

<224> -DIP 2C Gene-specific amplification primer 1

<400>4

caacagccatttgctgagag

<210>5

<211>20

<212>DNA

<213> Artificial sequence

<220>

<224> -DIP 2C Gene-specific amplification primer 2

<400>5

ctctagtttcttgggtgtag

Claims (5)

1. The application of a reagent for detecting mutation of a MYH6 mutant gene or a MYH6 gene expression product in preparing a congenital heart disease detection product; the product comprises a reagent for detecting mutation of a MYH6 mutant gene or a MYH6 gene expression product; the detection method of the product is at least one of direct nucleic acid sequencing, nucleic acid hybridization, nucleic acid amplification, nucleic acid probe or immunodetection; the product comprises a preparation, a chip or a kit; the nucleotide sequence of the MYH6 mutant gene is shown in SEQ ID NO.1, and the nucleotide sequence has a mutation from G to A at position 1576; the amino acid sequence of the mutation of the MYH6 gene expression product is shown in SEQ ID NO.2, and the amino acid sequence has a mutation from Glu to Lys at position 526.

2. The use according to claim 1, wherein the test sample of the product is peripheral blood.

3. The use according to claim 2, wherein the detection reagent is a probe having a nucleotide sequence shown as SEQ ID No. 3.

4. The use according to claim 1, wherein primers having the nucleotide sequences shown in SEQ ID No.4 and SEQ ID No.5 are used in the nucleic acid amplification.

5. A method for constructing a congenital heart disease model, which is characterized in that a reagent for expressing a MYH6 mutant gene is applied to a culture system, wherein the culture system is selected from a cell system, a tissue system or an organ system, the nucleotide sequence of the MYH6 mutant gene is shown in SEQ ID NO.1, and the nucleotide has a mutation from G to A at position 1576; the reagent for expressing the MYH6 mutant gene is MYH6 adenovirus.

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