CN110967322A - Blood cell analyzer and blood cell analyzing method - Google Patents
Blood cell analyzer and blood cell analyzing method Download PDFInfo
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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Abstract
The application discloses hematology analyzer and hematology analysis method, this hematology analyzer includes: the device comprises a blood sample collecting device, a reagent providing device, a blood sample reaction device and a blood sample detection device, wherein the blood sample collecting device is used for collecting and providing a blood sample to be detected; the reagent supply device is used for supplying reagents used for analyzing the blood sample to be tested; the blood sample reaction device is used for receiving the blood sample to be detected provided by the blood sample collection device and the reagent provided by the reagent providing device, and mixing and reacting the blood sample to be detected and the reagent to be detected to obtain a reacted blood sample to be detected; and the blood sample detection device is used for receiving the reacted blood sample to be detected, and performing blood cell analysis and blood type detection on the reacted blood sample to be detected so as to obtain blood conventional parameters and a blood type detection result of the blood sample to be detected. In this way, this application can realize blood conventionality and blood group and detect simultaneously on hematology analyzer, and reduce patient's misery.
Description
Technical Field
The present application relates to the field of blood cell analysis technology, and in particular, to a blood cell analyzer and a blood cell analysis method.
Background
Currently, when routine blood type and routine blood detection is performed, the detection of the blood type is usually performed by a single blood type analyzer, and the detection is performed by another single three-classification or five-classification blood cell analyzer.
Under the above conditions, two detections need to be separately performed, and the working efficiency is low; and the same patient takes samples twice, which increases the pain of the patient.
Disclosure of Invention
The technical problem that this application mainly solved provides a hematology analyzer and blood cell analysis method, can realize the blood conventionality and blood type and detect simultaneously on the hematology analyzer, and reduce patient's misery.
In order to solve the technical problem, the application adopts a technical scheme that: provided is a blood cell analyzer including: the blood sample collecting device is used for collecting and providing a blood sample to be detected; a reagent providing device for providing a reagent for analyzing the blood sample to be tested; the blood sample reaction device is used for receiving the blood sample to be detected provided by the blood sample collection device and the reagent provided by the reagent providing device, mixing and reacting to obtain a reacted blood sample to be detected; and the blood sample detection device is used for receiving the reacted blood sample to be detected, and performing blood cell analysis and blood type detection on the reacted blood sample to be detected so as to obtain blood conventional parameters and a blood type detection result of the blood sample to be detected.
In order to solve the above technical problem, another technical solution adopted by the present application is: provided is a blood cell analysis method for a blood cell analyzer including a blood sample collection device, a reagent supply device, a blood sample reaction device, and a blood sample detection device, the method including: the blood sample collection device collects a blood sample to be tested and provides the blood sample to be tested for the blood sample reaction device, and the reagent providing device provides a reagent for analyzing the blood sample to be tested for the blood sample reaction device; the blood sample reaction device receives the blood sample to be detected and the reagent respectively, and performs mixing and reaction to obtain a reacted blood sample to be detected, and analyzes the blood sample to be detected to the blood sample detection device; and the blood sample detection device receives the reacted blood sample to be detected, and performs blood cell analysis and blood type detection on the reacted blood sample to be detected to obtain blood conventional parameters and a blood type detection result of the blood sample to be detected.
The beneficial effect of this application is: different from the situation of the prior art, the blood sample detection device in the blood cell analyzer can perform blood cell analysis and blood type detection on the reacted blood sample to be detected, so that the blood routine parameters and the blood type detection result of the blood sample to be detected are obtained, and the blood routine and the blood type can be simultaneously detected on the same blood cell analyzer; and only need draw the sample once from the patient to reduce patient's misery.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic structural view of an embodiment of a blood cell analyzer according to the present application;
FIG. 2 is a schematic structural view of another embodiment of the blood cell analyzer of the present application;
FIG. 3 is a schematic configuration diagram of a blood cell analyzer according to another embodiment of the present application;
FIG. 4 is a schematic view of a fluorescence optical detection system according to an embodiment of the blood cell analyzer of the present application;
FIG. 5 is a schematic flow chart diagram of one embodiment of the blood cell analysis method of the present application;
FIG. 6 is a schematic flow chart diagram of another embodiment of the blood cell analysis method of the present application;
FIG. 7 is a schematic flow chart showing a blood cell analysis method according to still another embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of a blood cell analyzer according to the present application, the blood cell analyzer includes: a blood sample collection device 10, a reagent supply device 20, a blood sample reaction device 30, and a blood sample detection device 40.
The blood sample collection device 10 is used for collecting and providing a blood sample to be tested. Specifically, before performing blood sample detection, a blood sample at a specific position of a patient may be collected through a blood collection tube, and a test tube rack on which the collection tube is placed is further placed in the blood cell analyzer in the present embodiment, and when performing blood sample detection, the blood sample collection device 10 may further collect a blood sample to be detected in the blood collection tube, and further provide the collected blood sample to be detected to the blood sample reaction device 30 under the control of a certain means.
The reagent supplying device 20 is used for supplying reagents used for analyzing a blood sample to be tested. It should be noted that the blood sample used for the blood sample test is not directly tested by the blood extracted from the patient, but the collected blood sample to be tested needs to be processed by a certain means, such as dilution, incubation, dissolution, etc., according to the test item to be tested, and then used. In this embodiment, a reagent supplying device 20 is further used to supply a certain amount of reagent and deliver it to the blood sample reaction device 30 for processing the blood sample to be tested.
The blood sample reaction device 30 is configured to receive the blood sample to be tested provided by the blood sample collection device 10 and the reagent provided by the reagent providing device 20, and mix and react the blood sample to be tested to obtain a reacted blood sample to be tested. As described above, in the present embodiment, the reagent supplied from the reagent supplying apparatus 20 is physically and/or chemically reacted with the sample blood, thereby obtaining a sample blood that can be used for the detection.
The blood sample detection device 40 is configured to receive the reacted blood sample to be detected, and perform blood cell analysis and blood type detection on the reacted blood sample to be detected to obtain blood routine parameters and a blood type detection result of the blood sample to be detected.
It should be noted that the blood sample detection device 40 in this embodiment can perform routine blood detection and blood type detection on the same device after performing reaction processing on a blood sample to be detected, so as to obtain routine bleeding parameters and blood type detection results, and do not need to perform detection separately, thereby improving the working efficiency; meanwhile, the patient pain can be reduced because only one blood sample is collected.
The blood sample collection device 10 can be implemented by various means when providing the blood sample to be tested to the blood sample reaction device 30, providing the reagent to the blood sample reaction device 30 by the reagent providing device 20, and providing the blood sample to be tested to the blood sample detection device 40 by the blood sample reaction device 30, for example, the blood sample collection device 10 or the reagent providing device 20 can be driven to a corresponding position and then the blood sample or the reagent to be tested is released in the corresponding blood sample reaction device 30, or other means can be implemented.
In one embodiment, referring to fig. 2 and 3, the blood cell analyzer further includes a distribution system 50. The distribution system 50 is used for distributing the blood sample to be tested in the blood sample collection device 10 and the reagent in the reagent providing device 20 to the blood sample reaction device 30, and distributing the blood sample to be tested after reaction in the blood sample reaction device 30 to the blood sample detection device 40.
In particular, the dispensing system 50 may be a "suck-and-push" device, for example, when it is desired to dispense a reagent in the reagent providing device 20 to the blood sample reaction device 30, an amount of the reagent may be "sucked" from the reagent providing device 20 through one end of the dispensing system 50, and further "pushed" out of the reagent into the blood sample reaction device 30 through the other end of the dispensing system 50. The "suction-discharge" of the dispensing system 50 in the present embodiment can be realized by providing a syringe, a fixed displacement pump, or the like in the dispensing system 50.
In the above manner, by using the distribution system 50, the liquids in the blood sample collection device 10, the reagent supply device 20 and the blood sample reaction device 30 can be automatically supplied to the respective devices without manual handling by an operator, thereby improving the work efficiency of blood cell detection.
Referring to fig. 2, in one embodiment, the blood sample reaction device 30 includes a leukocyte classification reaction chamber 31, an a reaction chamber 32, a B reaction chamber 33 and an H reaction chamber 34, and the blood sample detection device 40 includes a leukocyte classification/blood type detection system 41.
The leucocyte classification reaction tank 31 is used for forming a blood sample to be tested for leucocyte classification detection, and the a anti-reaction tank 32, the B anti-reaction tank 33 and the H anti-reaction tank 34 are used for forming a blood sample to be tested for blood type detection. Specifically, the a-antibody reaction pool 32 is used for the binding reaction between the blood sample to be tested and the a-antibody, the B-antibody reaction pool 33 is used for the binding reaction between the blood sample to be tested and the B-antibody, and the H-antibody reaction pool 34 is used for the binding reaction between the blood sample to be tested and the H-antibody.
Specifically, the blood sample to be tested in the blood sample collection device 10 and the reagent in the reagent supply device 20 can be distributed to the leukocyte classification reaction pool 31, the a anti-reaction pool 32, the B anti-reaction pool 33 and the H anti-reaction pool 34 through the distribution system 50, so as to obtain the leukocyte classification blood sample to be tested, the blood sample to be tested after a binding of a anti-binding, the blood sample to be tested after B binding, and the blood sample to be tested after H binding.
Further, the reagent supplying device 20 includes a diluent supplying unit 21, a leukocyte classification hemolytic agent supplying unit 22, an a anti-reagent supplying unit 23, a B anti-reagent supplying unit 24, and an H anti-reagent supplying unit 25. Wherein, the diluent providing unit 21 is used for providing a diluent, the leukocyte classification hemolytic agent providing unit 22 is used for providing a leukocyte classification hemolytic agent, the a-antibody reagent providing unit 23 is used for providing an a-antibody reagent, the B-antibody reagent providing unit 24 is used for providing a B-antibody reagent, and the H-antibody reagent providing unit 25 is used for providing an H-antibody reagent.
Wherein, A antibody, B antibody and H antibody respectively refer to antibodies which can be combined with A antigen, B antigen and H antigen on the surface of red blood cells in blood. In this embodiment, the combination reaction of the antibody A, the antibody B and the antibody H is performed in the antibody A reaction tank, the antibody B reaction tank and the antibody H reaction tank, the expression intensities of the three antigens can be sequentially evaluated A, B, H through the leukocyte classification/blood type detection system 41 after the reaction is completed, and the blood type of the blood sample to be detected can be obtained through the difference of the expression intensities of the three antigens.
More specifically, the dispensing system 50 may dispense pairs of blood samples, reagents, etc. to be tested according to specific testing items. For example, the distribution system 50 may distribute the diluent in the diluent providing unit 21 to the leukocyte classification reaction tank 31, the a anti-reaction tank 32, the B anti-reaction tank 33, and the H anti-reaction tank 34, distribute the blood sample to be tested in the blood sample collection device 10 to the above reaction tanks, and correspondingly distribute the leukocyte classification hemolytic agent in the leukocyte classification hemolytic agent providing unit 22, the a anti-reagent in the a anti-reagent providing unit 23, the B anti-reagent in the B anti-reagent providing unit 24, and the H anti-reagent in the H anti-reagent providing unit 25 to the leukocyte classification reaction tank 31, the a anti-reaction tank 32, the B anti-reaction tank 33, and the H anti-reaction tank 34, so that the blood sample to be tested is obtained through reaction in each reaction tank.
After obtaining each blood sample to be tested, the obtained leukocyte classification blood sample to be tested, the blood sample to be tested after a binding resistance, the blood sample to be tested after B binding resistance, and the blood sample to be tested after H binding resistance can be sent to the leukocyte classification/blood type detection system 41 for detection through the distribution system 50 in a time sharing manner, so as to obtain a leukocyte classification detection result and a blood type detection result.
It is easy to understand that, in the present embodiment, the four blood samples to be tested are all tested in the white blood cell sorting/blood type testing system 41, so that the testing needs to be performed in time division, and the specific testing sequence is not limited.
Referring further to fig. 3, in another embodiment, the blood sample reaction device 30 includes a leukocyte classification/blood type detection reaction cell 35, wherein, the leucocyte classification/blood type detection reaction pool 35 is time-sharing multiplexed, the blood sample to be detected in the blood sample collection device 10 and the reagent in the reagent providing device 20 are distributed to the leucocyte classification/blood type detection reaction pool 35 through the distribution system 50, obtaining a blood sample to be detected by classifying leucocytes, a blood sample to be detected after A anti-binding, a blood sample to be detected after B anti-binding and a blood sample to be detected after H anti-binding in a time-sharing manner, and further sends the blood sample to be tested after leukocyte classification, the blood sample to be tested after A anti-binding, the blood sample to be tested after B anti-binding and the blood sample to be tested after H anti-binding to the leukocyte classification/blood type detection system 41 for detection through the distribution system 50 in a time-sharing manner, so as to obtain a leukocyte classification detection result and a blood type detection result.
Specifically, the distribution system 50 distributes the diluent in the diluent providing unit 21, the blood sample to be detected in the blood sample collecting device 10, and the leukocyte hemolytic agent in the leukocyte classification hemolytic agent providing unit 22 to the leukocyte classification/blood type detection reaction tank 35 in sequence, so as to obtain the leukocyte classification blood sample to be detected, and then sends the obtained leukocyte classification blood sample to be detected to the leukocyte classification/blood type detection system 41 for detection; then, the diluent in the diluent providing unit 21, the blood sample to be detected in the blood sample collecting device 10 and the anti-reagent a in the anti-reagent a providing unit 23 are sequentially distributed to the leukocyte classification/blood type detection reaction pool 35 through the distributing system 50, so that the blood sample to be detected after anti-binding of A is obtained through mixing reaction, and the obtained blood sample to be detected after anti-binding of A is sent to the leukocyte classification/blood type detection system 41 for detection; and then, obtaining and detecting the blood sample to be detected after the B antibody is combined and obtaining and detecting the blood sample to be detected after the H antibody is combined in sequence, wherein the specific processes of obtaining and detecting the blood sample to be detected after the A antibody is combined are the same, and are not described again here. It should be noted that the blood sample to be tested after leukocyte classification, the blood sample to be tested after a binding of the antibody, the blood sample to be tested after B binding of the antibody and the blood sample to be tested after H binding of the antibody are sent to the leukocyte classification/blood type detection system 41 in different time periods, which are not in sequence, and the above is only used as an example.
Referring further to fig. 4, in one application scenario, the leukocyte classification/blood type detection system 41 is a fluorescence optical detection system 411. Both the leukocyte classification and blood type detection are performed in the fluorescence optical detection system 411. Specifically, fluorescence optical detection system 411 includes a flow cell 411a, a laser 411b, a scattered signal collection device 411c, a fluorescence signal collection device 411d, a laser adjustment device, and a control board card.
The blood sample to be detected for classifying the leucocytes is sent into a fluorescence optical detection system 411 and then flows into a flow chamber 411a, flows through the flow chamber under the wrapping of sheath fluid, and is excited to generate fluorescence and generate scattered light under the laser emitted by a laser 411b, two optical channels are arranged, and one channel collects the scattered signal through a scattered signal collection device 411 c; the other optical channel collects the fluorescence signal by the fluorescence signal collecting means 411 d. The scattering signal collecting device 411c may collect three signals, i.e., a low-angle scattering signal, a medium-angle scattering signal, and a high-angle scattering signal, and in this embodiment, the detection may be performed by acquiring any two or more signals of the three scattering signals and the fluorescence signal, and the white blood cells are classified into five categories by feature differences, so as to obtain a detection result of the five categories of the white blood cells.
It should be noted that the antibodies in the A antibody reagent, the B antibody reagent and the H antibody reagent are labeled with fluorescent light emitting agents and bind to the red blood cells in the blood sample to be tested through the pairing of the antigen and the antibody.
Further, taking the blood sample to be detected after the a antibody is bound as an example, after the blood sample to be detected after the a antibody is bound is sent into the flow chamber 411a of the fluorescence optical detection system 411, the cells are controlled to line up one by one through the flow cytometry, the laser 411b emits laser to the detection area, so that the fluorescence emitting agent on the a antibody is excited to emit fluorescence, and then the fluorescence information is collected through the fluorescence signal collecting device 411d to obtain the expression intensity of the a antibody. Similarly, the expression intensities of the B antibody and the H antibody can be further obtained respectively, and then the fluorescence optical detection system 411 can further obtain the blood type corresponding to the blood sample to be detected according to the obtained expression intensities of the a antibody, the B antibody and the H antibody.
It should be noted that, in the application scenario, the ratio of the expression intensity of the antibody a to the expression intensity of the antibody H and the ratio of the expression intensity of the antibody B to the expression intensity of the antibody H can be further obtained according to the expression intensities of the antibody a, the antibody B and the antibody H, and the sub-blood type corresponding to the blood sample to be detected can be further obtained, so that the blood type detection result is more comprehensive and accurate.
In one embodiment, the blood sample reaction device 30 further includes a leukocyte/hemoglobin reaction cell 36, the blood sample detection device 40 further includes a leukocyte count detection system 42 and a hemoglobin content detection system 43, and the leukocyte/hemoglobin reaction cell 36 receives the blood sample to be detected and the reagent provided by the blood sample collection device 10 and the reagent providing device 20, respectively, to obtain a leukocyte/hemoglobin blood sample to be detected.
Specifically, the blood sample to be tested containing leucocytes/hemoglobin obtained in the present embodiment can be used for both leucocyte count detection and hemoglobin content detection, and in the present embodiment, the reagent providing apparatus 20 further includes a hemoglobin hemolytic agent providing unit 26, where the hemoglobin hemolytic agent providing unit 26 is used for providing a hemoglobin hemolytic agent, and the hemoglobin hemolytic agent can lyse the erythrocytes in the blood sample to be tested, and further release hemoglobin for leucocyte count detection and hemoglobin content detection.
Specifically, while obtaining the blood sample to be tested for leukocyte classification and the blood type test, the distribution system 50 may distribute the diluent in the diluent providing unit 21, the blood sample to be tested in the blood sample collecting device 10, and the hemoglobin hemolysis in the hemoglobin hemolysis providing unit 26 to the leukocyte/hemoglobin reaction cell 36, and then obtain the leukocyte/hemoglobin blood sample to be tested after mixing reaction.
Further, after obtaining the blood sample to be tested for leukocyte/hemoglobin, the leukocyte count test and the hemoglobin content test can be performed in various ways. In an application scenario, in the same manner as the above-mentioned leukocyte classification and blood type detection, a leukocyte/hemoglobin blood sample to be detected is respectively sent to the leukocyte count detection system 42 and the hemoglobin content detection system 43 through the distribution system 50, so as to perform detection to obtain a leukocyte count detection result and a hemoglobin content detection result; in another application scenario, the device for counting white blood cells and detecting hemoglobin content is directly disposed in the white blood cell/hemoglobin reaction tank 36, so as to form a white blood cell counting detection system 42 and a hemoglobin content detection system 43 together with the white blood cell/hemoglobin reaction tank 36, so that the white blood cell/hemoglobin to-be-detected blood sample can be directly detected after reaction.
In the present embodiment, the white blood cell count detecting system 42 can count the total number of white blood cells in the white blood cell/hemoglobin test blood sample by an electrical impedance method, and the hemoglobin content detecting system 43 can detect the hemoglobin content in the white blood cell/hemoglobin test blood sample by a colorimetric method. Of course, in other embodiments, the detection may be performed in other manners, and is not limited herein.
In one embodiment, the blood sample reaction device 30 further includes a red blood cell/platelet reaction chamber 37, the blood sample detection device 40 further includes a red blood cell/platelet count detection system 44, the red blood cell/platelet reaction chamber 37 receives the blood sample to be detected and the reagent provided by the blood sample collection device 10 and the reagent providing device 20 respectively to obtain the blood sample to be detected and further sends the blood sample to be detected to the red blood cell/platelet count detection system 44 for detection to obtain a red blood cell count detection result and a platelet count detection result.
It should be noted that the blood cells in the blood are mainly divided into red blood cells, white blood cells and platelets, and the number of white blood cells is small and can be ignored compared with the number of red blood cells and platelets, so that the white blood cells do not need to be dissolved by adding a corresponding white blood cell dissolving agent into the blood sample to be tested. Specifically, when a blood sample to be tested for red blood cells/platelets is obtained, the diluent in the diluent providing unit 21 and the blood sample to be tested in the blood sample collection device 10 can be distributed to the red blood cells/platelets reaction pool 37 through the distribution system 50 to dilute the blood sample to be tested to obtain a corresponding blood sample to be tested for red blood cells/platelets.
In the present embodiment, the counting of both red blood cells and platelets is performed by the electrical impedance method, and since the volume difference between red blood cells and platelets is large, the same detection system can be used for simultaneous detection. Specifically, the red blood cell/platelet count detection device and the red blood cell/platelet reaction tank 37 together form a red blood cell/platelet count detection system 44, and count red blood cells and platelets simultaneously.
Of course, in other embodiments, the red blood cell/platelet count detection system 44 may be provided in two parts separate from the red blood cell/platelet reaction chamber 37, and the blood sample to be tested for red blood cells/platelets may be sent to the red blood cell/platelet count detection system 44 for counting by the distribution system 50. In addition, the red blood cell count and the platelet count may be performed in two different blood sample detection devices 40, respectively, and are not particularly limited herein.
In an embodiment, the blood cell analyzer further includes a blood sample collecting device cleaning device 60, the blood sample collecting device cleaning device 60 is disposed at the periphery of the blood sample collecting device 10, and is used for cleaning the blood sample collecting device 10 after the blood sample collecting device 10 collects a blood sample to be measured or provides the blood sample to be measured to the blood sample reaction device 30.
It is easy to understand that after the blood sample collection device 10 collects the blood sample to be tested, or the blood sample to be tested is provided to the blood sample reaction device 30, blood stains are easily left at the outlet of the blood sample to be tested, and in order to avoid cross infection, the blood sample to be tested can be cleaned by the blood sample collection device cleaning device 60 after each of the above actions is performed, so as to keep the blood sample clean.
Specifically, the blood sample collection device cleaning device 60 may be provided with an inlet and an outlet, with the cleaning fluid entering through the inlet and exiting through the outlet and exiting the exterior of the apparatus through the waste fluid discharge device 60, thereby completing the cleaning of the blood sample collection device 10 during this process. In one application scenario, the diluent in the diluent providing unit 21 may be further distributed to the blood sample collection device washing device 60 through the distribution system 50, and the blood sample collection device 10 may be washed by using the diluent as a washing solution.
In this embodiment, the blood sample collection device 10 may be a sampling needle.
In addition, during and after the above-mentioned tests, the blood sample reaction device 30 and the test system need to be cleaned. In one embodiment, after the blood sample to be tested reacted in each of the blood sample reaction devices 30 is fed into the corresponding blood sample testing device 40, an amount of the diluent in the diluent providing unit 21 can be dispensed to the blood sample reaction device 30 by the dispensing system 50 for cleaning. It should be noted that the above-mentioned embodiment of the leukocyte classification/blood type detection reaction cell 35 is time-division multiplexed, and after a blood sample to be tested is obtained by using the leukocyte classification/blood type detection reaction cell 35 each time, the leukocyte classification/blood type detection reaction cell needs to be cleaned to keep clean. Similarly, after the blood sample detection device 40 finishes the detection, the predetermined amount of the diluent in the diluent supply unit 21 needs to be distributed to the blood sample detection device 40 by the distribution system 50 to be cleaned.
Further, in one embodiment, the blood cell analyzer further includes a waste liquid discharge device 70 for discharging waste liquid generated by the blood sample reaction device 30, the blood sample detection device 40 and the blood sample collection device washing device 60.
As will be understood, when the blood sample reaction device 30, the blood sample detection device 40 and the blood sample collection device 10 are cleaned, a certain amount of waste liquid is generated, and in this embodiment, the blood sample reaction device 30, the blood sample detection device 40 and the blood sample collection device cleaning device 60 are further communicated with the waste liquid discharge device 70, so that the generated waste liquid is discharged into the waste liquid discharge device 70 and further discharged to the outside of the hematology analyzer.
Referring to fig. 1 and 5 together, fig. 5 is a schematic flow chart of an embodiment of the blood cell analysis method of the present application. The blood cell analysis method according to the present embodiment is applied to the blood cell analyzer according to the embodiment of the blood cell analyzer according to the present application, and the specific configuration of the blood cell analyzer is the same as that of the embodiment of the blood cell analyzer, and thus, the description thereof is omitted. The blood cell analysis method according to the present embodiment includes:
step S11: the blood sample collection device 10 collects a blood sample to be tested and provides the blood sample to be tested to the blood sample reaction device 30, and the reagent providing device 20 provides a reagent used for analyzing the blood sample to be tested to the blood sample reaction device 30;
step S12: the blood sample reaction device 30 receives the blood sample to be tested and the reagent, respectively, and performs mixing and reaction to obtain a reacted blood sample to be tested, and sends the reacted blood sample to be tested to the blood sample detection device 40;
step S13: the blood sample detection device 40 receives the reacted blood sample to be detected, and performs blood cell analysis and blood type detection on the reacted blood sample to be detected to obtain blood routine parameters and a blood type detection result of the blood sample to be detected.
The structure and technical effects of the blood cell analyzer according to the embodiment of the blood cell analysis method are similar to those of the embodiment of the blood cell analyzer, and for details, reference is made to the embodiment and no further description is given here.
In one embodiment, referring to fig. 2 and 6 together, when the blood cell analyzer in fig. 2 is applied, the blood cell analyzing method in this embodiment includes:
step S21: the blood sample collecting device 10 collects a blood sample to be measured, and after the collection is completed, the distribution system 50 distributes a diluent to the blood sample collecting device cleaning device 60 to clean the blood sample collecting device 10 and discharge the generated waste liquid to the waste liquid discharge device 70;
step S22: a certain amount of diluent is respectively distributed in the leukocyte classification reaction tank 31, the A anti-reaction tank 32, the B anti-reaction tank 33, the H anti-reaction tank 34, the leukocyte/hemoglobin reaction tank 36 and the erythrocyte/platelet reaction tank 37 through the distribution system 50, then a certain amount of blood sample to be detected is respectively sent into the reaction tanks through the blood sample collection device 10 and the distribution system 50, the diluent is distributed to the blood sample collection device cleaning device 60 through the distribution system 50, the blood sample collection device 10 is cleaned, and the generated waste liquid is discharged to the waste liquid discharge device 70;
step S23: a leukocyte classification hemolytic agent, an A anti-reagent, a B anti-reagent, an H anti-reagent and a hemoglobin hemolytic agent are correspondingly added into the leukocyte classification reaction tank 31, the A anti-reaction tank 32, the B anti-reaction tank 33, the H anti-reaction tank 34 and the leukocyte/hemoglobin reaction tank 36 respectively through a distribution system 50 to react, so that a leukocyte classification blood sample to be detected, an A anti-combined blood sample to be detected, a B anti-combined blood sample to be detected, an H anti-combined blood sample to be detected, a leukocyte/hemoglobin blood sample to be detected and a red blood cell/platelet blood sample to be detected are obtained respectively;
step S24: carrying out white blood cell counting and hemoglobin content measurement in a white blood cell/hemoglobin reaction tank 36, simultaneously carrying out red blood cell counting and platelet counting in a red blood cell/platelet reaction tank 37, and simultaneously distributing a white blood cell classification blood sample to be detected, an A anti-binding blood sample to be detected, a B anti-binding blood sample to be detected and an H anti-binding blood sample to be detected to a fluorescence optical detection system 411 for five-classification detection of white blood cells and blood type detection in a time-sharing manner through a distribution system 50;
step S25: after the analysis and detection are completed, outputting the conventional blood parameters and the blood type detection result, cleaning each reaction cell and the detection system, and discharging the generated waste liquid to the waste liquid discharge device 70.
The structure and technical effects of the blood cell analyzer according to the embodiment of the blood cell analysis method are similar to those of the embodiment of the blood cell analyzer, and for details, reference is made to the embodiment and no further description is given here.
In one embodiment, referring to fig. 3 and 7 together, when the blood cell analyzer in fig. 3 is applied, the blood cell analyzing method in this embodiment includes:
step S31: the blood sample collecting device 10 collects a blood sample to be measured, and after the collection is completed, the distribution system 50 distributes a diluent to the blood sample collecting device cleaning device 60 to clean the blood sample collecting device 10 and discharge the generated waste liquid to the waste liquid discharge device 70;
step S32: a certain amount of diluent is respectively distributed into the leukocyte classification/blood type detection reaction tank 35, the leukocyte/hemoglobin reaction tank 36 and the erythrocyte/platelet reaction tank 37 through the distribution system 50, then a certain amount of blood sample to be detected is respectively sent into the reaction tanks through the blood sample collection device 10 and the distribution system 50, the diluent is distributed into the blood sample collection device cleaning device 60 through the distribution system 50, the blood sample collection device 10 is cleaned, and the generated waste liquid is discharged to the waste liquid discharge device 70;
step S33: a leukocyte classification hemolytic agent and a hemoglobin hemolytic agent are respectively and correspondingly added into the leukocyte classification reaction tank 31 and the leukocyte/hemoglobin reaction tank 36 through a distribution system 50 to react, so as to obtain a leukocyte classification blood sample to be detected, a leukocyte/hemoglobin blood sample to be detected and a red blood cell/platelet blood sample to be detected;
step S34: white blood cell counting and hemoglobin content measurement are carried out in the white blood cell/hemoglobin reaction tank 36, meanwhile, red blood cell counting and platelet counting are carried out in the red blood cell/platelet white reaction tank, and meanwhile, blood samples to be detected for white blood cell classification are distributed to the fluorescence optical detection system 411 for white blood cell five classification detection in time division through the distribution system 50;
step S35: after the analysis and detection are finished, outputting conventional blood parameters, cleaning each reaction cell and the detection system, and discharging the generated waste liquid to a waste liquid discharge device 70;
step S36: sending corresponding diluent, blood sample to be detected and A antibody reagent or diluent, blood sample to be detected and B antibody reagent or diluent, blood sample to be detected and H antibody reagent to the leukocyte classification/blood type detection reaction pool 35 in a time-sharing manner to obtain blood sample to be detected after A antibody is combined or blood sample to be detected after B antibody is combined or blood sample to be detected after H antibody is combined, and sending the blood sample to be detected into the fluorescence optical detection system 411 for detection;
step S37: after the analysis and detection are completed, the blood type detection result is outputted, the blood sample collection device 10, the leukocyte classification/blood type detection reaction tank 35 and the fluorescence optical detection system 411 are cleaned, and the generated waste liquid is discharged to the waste liquid discharge device 70.
It should be noted that, in this embodiment, the four tests included in the leukocyte classification test and the blood type test are not in sequence, and the above steps are only one of the embodiments.
The structure and technical effects of the blood cell analyzer according to the embodiment of the blood cell analysis method are similar to those of the embodiment of the blood cell analyzer, and for details, reference is made to the embodiment and no further description is given here.
The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (11)
1. A blood cell analyzer, comprising:
the blood sample collecting device is used for collecting and providing a blood sample to be detected;
a reagent providing device for providing a reagent for analyzing the blood sample to be tested;
the blood sample reaction device is used for receiving the blood sample to be detected provided by the blood sample collection device and the reagent provided by the reagent providing device, mixing and reacting to obtain a reacted blood sample to be detected;
and the blood sample detection device is used for receiving the reacted blood sample to be detected, and performing blood cell analysis and blood type detection on the reacted blood sample to be detected so as to obtain blood conventional parameters and a blood type detection result of the blood sample to be detected.
2. The hematology analyzer of claim 1, further comprising a dispensing system;
the distribution system is used for distributing the blood sample to be detected in the blood sample collection device and the reagent in the reagent providing device to the blood sample reaction device respectively, and distributing the reacted blood sample to be detected in the blood sample reaction device to the blood sample detection device.
3. The hematology analyzer of claim 2, wherein the blood sample reaction device comprises a leukocyte classification reaction cell, an A anti-reaction cell, a B anti-reaction cell and an H anti-reaction cell, and the blood sample detection device comprises a leukocyte classification/blood type detection system;
the blood sample to be detected in the blood sample collection device and the reagent in the reagent supply device are distributed to the leukocyte classification reaction pool, the A anti-reaction pool, the B anti-reaction pool and the H anti-reaction pool through the distribution system, so that a leukocyte classification blood sample to be detected, a blood sample to be detected after A anti-binding, a blood sample to be detected after B anti-binding and a blood sample to be detected after H anti-binding are obtained, and the leukocyte classification blood sample to be detected, the blood sample to be detected after A anti-binding, the blood sample to be detected after B anti-binding and the blood sample to be detected after H anti-binding are further distributed to the leukocyte classification/blood type detection system through the distribution system to be detected, so that a leukocyte classification detection result, an A type blood detection result, a B type blood detection result and an O type blood detection result are obtained.
4. The hematology analyzer of claim 2, wherein the blood sample reaction device comprises a leukocyte classification/blood type detection reaction cell, and the blood sample analysis device comprises a leukocyte classification/blood type detection system;
wherein, the leucocyte classification/blood group detection reaction pool is time-sharing multiplexed, the blood sample to be detected in the blood sample collection device and the reagent in the reagent providing device are distributed into the leucocyte classification/blood group detection reaction pool through the distribution system, obtaining a blood sample to be detected by classifying leucocytes, a blood sample to be detected after A anti-binding, a blood sample to be detected after B anti-binding and a blood sample to be detected after H anti-binding in a time-sharing manner, and further distributing the blood sample to be detected after the leucocyte classification, the blood sample to be detected after the A anti-binding, the blood sample to be detected after the B anti-binding and the blood sample to be detected after the H anti-binding to the leucocyte classification/blood type detection system for detection in a time-sharing manner through the distribution system, so as to obtain a leucocyte classification detection result, an A type blood detection result, a B type blood detection result and an O type blood detection result.
5. The blood cell analyzer of claim 3 or 4, wherein the leukocyte classification/blood group detection system is a fluorescence optical detection system.
6. The blood cell analyzer of claim 1, wherein the blood sample reaction device further comprises a leukocyte/hemoglobin reaction cell, and the blood sample detection device further comprises a leukocyte count detection system and a hemoglobin content detection system;
the leucocyte/hemoglobin reaction tank receives the blood sample to be detected and the reagent which are respectively provided by the blood sample collecting device and the reagent providing device so as to obtain a leucocyte/hemoglobin blood sample to be detected, and further sends the leucocyte/hemoglobin blood sample to be detected to the leucocyte counting detection system and the hemoglobin content detection system so as to respectively detect to obtain a leucocyte counting detection result and a hemoglobin content detection result.
7. The blood cell analyzer of claim 1, wherein the blood sample reaction device further comprises a red blood cell/platelet reaction cell, and the blood sample detection device further comprises a red blood cell/platelet count detection system;
the red blood cell/platelet reaction pool receives the blood sample to be detected and the reagent which are respectively provided by the blood sample collecting device and the reagent providing device so as to obtain the blood sample to be detected of the red blood cells/platelets, and further sends the blood sample to be detected of the red blood cells/platelets to the red blood cell/platelet counting and detecting system so as to carry out detection to obtain a red blood cell counting and detecting result and a platelet counting and detecting result.
8. The blood cell analyzer according to claim 1, wherein the reagent supplying means includes a diluent supplying unit, a leukocyte classification hemolytic agent supplying unit, a hemoglobin hemolytic agent supplying unit, an a-antibody reagent supplying unit, a B-antibody reagent supplying unit, an H-antibody reagent supplying unit;
the reagent supplying unit is used for supplying a reagent for leukocyte classification, the hemoglobin hemolytic agent supplying unit is used for supplying a hemoglobin hemolytic agent, the A-antibody reagent supplying unit is used for supplying an A-antibody reagent, the B-antibody reagent supplying unit is used for supplying a B-antibody reagent, and the H-antibody reagent supplying unit is used for supplying an H-antibody reagent.
9. The hematology analyzer of claim 1, further comprising a blood sample collection device cleaning device disposed at a periphery of the blood sample collection device for cleaning the blood sample collection device after the blood sample collection device collects the blood sample to be tested or provides the blood sample to be tested to the blood sample reaction device.
10. The hematology analyzer of claim 9, further comprising a waste liquid discharge device for discharging waste liquid generated by the blood sample reaction device, the blood sample detection device and the blood sample collection device cleaning device.
11. A blood cell analysis method for a blood cell analyzer including a blood sample collection device, a reagent supply device, a blood sample reaction device, and a blood sample detection device, the method comprising:
the blood sample collection device collects a blood sample to be tested and provides the blood sample to be tested for the blood sample reaction device, and the reagent providing device provides a reagent for analyzing the blood sample to be tested for the blood sample reaction device;
the blood sample reaction device receives the blood sample to be detected and the reagent respectively, and performs mixing and reaction to obtain a reacted blood sample to be detected, and sends the reacted blood sample to be detected to the blood sample detection device;
and the blood sample detection device receives the reacted blood sample to be detected, and performs blood cell analysis and blood type detection on the reacted blood sample to be detected to obtain blood conventional parameters and a blood type detection result of the blood sample to be detected.
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