CN110726793B - Isotope mass spectrum sample introduction system for researching photosynthesis gas exchange - Google Patents
Isotope mass spectrum sample introduction system for researching photosynthesis gas exchange Download PDFInfo
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- CN110726793B CN110726793B CN201911139936.1A CN201911139936A CN110726793B CN 110726793 B CN110726793 B CN 110726793B CN 201911139936 A CN201911139936 A CN 201911139936A CN 110726793 B CN110726793 B CN 110726793B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/24—Automatic injection systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
Abstract
The invention discloses an isotope mass spectrometry sample introduction system for researching photosynthesis gas exchange, which comprises a photosynthesis leaf chamber simulation device, a cold trap and an isotope mass spectrometer, wherein the photosynthesis leaf chamber simulation device comprises a temperature control base, an optical channel, a gas channel, a photosynthesis simulator and a semipermeable membrane. The invention discloses an isotope mass spectrum sample introduction system for researching photosynthesis gas exchange, which designs a photosynthetic leaf chamber simulation device in a laboratory environment by simulating green plant photosynthesis, and artificially and controllably introduces O2、CO2、H2O and light delivery to the green leaf and selective passage of O using a semi-permeable membrane2、CO2The formed mixed gas is purified by using a cold trap, and finally detected by using an isotope mass spectrometer, so that the carbon and oxygen isotope change tracks in the process of in-out of the photosynthesis gas can be automatically and continuously monitored on line, and the carbon assimilation dynamic process in a natural state can be researched.
Description
Technical Field
The invention relates to the technical field of isotope tracing, in particular to an isotope mass spectrum sample introduction system for researching photosynthesis gas exchange.
Background
Photosynthesis is that green plants utilize light energy to emit CO2And H2O synthesis of organic matter with simultaneous release of O2The physiological process of (1). The general process is as follows: first, CO2Diffusing from the outside atmosphere to the surface layer of the blade; then, the air hole passes through the air hole to reach the lower cavity of the air hole; finally, the chloroplast carboxylation site is reached for assimilation by carboxylase. However, the efficiency of plant photosynthesis is very low, converting only about 3% of the solar energy into usable forms.
The isotope tracer technique has the characteristics of rapid detection and accurate result, the application of the isotope tracer technique relates to a plurality of ecological research fields and becomes one of the most effective research means in the fields of ecology and environmental science (Barbour, M.M., Evans, J.R., Simonin, K.A.and Von Cammer, S.,2016.Online CO)2 and H2O oxygen isotope fractionation allows estimation of mesophyll conductance in C4 plants,and reveals that mesophyll conductance decreases as leaves age in both C4 and C3plant, New Phytologist,210: 875-889). The existing instruments for isotope analysis mainly include isotope mass spectrometers, laser isotope analyzers, film sample injection mass spectrometers and the like. The isotope mass spectrometer has the advantages of high test precision, good stability, more sample feeding devices, capability of realizing isotope analysis of solid, liquid and gas samples in environmental samples and the like, and is widely applied.
Adopt isotope mass spectrometer to carry out the automatic tracer monitoring in succession of on line that C and O isotope change to photosynthetic sample, can: 1) accurate determination of mesophyll conductance gm, studies have shown that CO2There are many resistances to the carboxylation site from the outside air, and the resistance (1/gm) of the liquid phase section diffusing from the air pore cavity to the carboxylation site is the rate-limiting step for restricting the photosynthetic efficiency, so it is obvious that the systematic study of the variation rule of the mesophyll conductance is of great significance for improving the photosynthetic efficiency. Research and development results of related technologies show that Mesophyll conductivity can be reliably obtained by measuring the fractionation effect of C isotope by using an isotope mass spectrometer, and other technologies and fitting methods have a plurality of premise assumptions, and the obtained results are unreliable (Flexas, J., Ribas-Carbo, M., Diaz-Espejo, A., Galmes, J.and Mederano, H.,2008. meso. phenol reduction to CO)2:current knowledge and future prospects.Plant,cell&environment,31: 602-); 2) mesophyllic conductance can be divided into transmembrane and cytosol (gl) conductance, and C and O isotopes are synchronously measured by using an isotope mass spectrometer, so that the contribution of two resistances to the mesophyllic conductance can be distinguished, which is the premise of deeply researching the mesophyllic conductance regulation mechanism (Barbour, M.M., Evans, J.R., Simonin, K.A. and Von Cammer, S.,2016.Online CO)2 and H2O oxygen isotope fractionation allows estimation of mesophyll conductance in C4 plants,and reveals that mesophyll conductance decreases as leaves age in both C4 and C3plant, New Phytologist,210: 875-; 3) research shows that the activity and the dynamic of carbonic anhydrase in the regulation and control of cell sap are key, and only isotope mass spectrometry is adopted at presentThe instrument monitors O isotope dynamic living body to measure carbonic anhydrase activity.
However, the commercial isotope mass spectrometer has high requirements for the precision and quality of sample injection materials, and the matched sample injector lacks a light source and a gas control system capable of performing photosynthesis, so that sample injection equipment capable of meeting the requirements is absent in the market at present, and further, the research of on-line automatic continuous tracing and monitoring of the C and O isotope changes in the photosynthesis process cannot be carried out.
Disclosure of Invention
The invention aims to provide an isotope mass spectrometry sample introduction system for researching photosynthesis gas exchange, which is used for solving the problem that the on-line automatic continuous tracing monitoring research on C and O isotope changes in the photosynthesis process cannot be carried out.
The invention provides an isotope mass spectrometry sample introduction system for researching photosynthesis gas exchange, which comprises a photosynthesis leaf chamber simulation device, a cold trap and an isotope mass spectrometer, wherein the photosynthesis leaf chamber simulation device, the cold trap and the isotope mass spectrometer are sequentially connected through a pipeline from front to back; a groove is formed in the temperature control base, and the photosynthetic simulator is arranged in the groove of the temperature control base; the semi-permeable membrane is arranged between the bottom surface of the photosynthetic simulator and the surface of the groove of the temperature control base; be provided with the leaf room in the photosynthetic simulator, gas passage includes air inlet, atmospheric pressure regulation mouth and outlet channel, the air inlet, atmospheric pressure regulation mouth, light channel with outlet channel all with the leaf room is linked together, outlet channel passes the temperature control base.
Preferably, the air inlet with the atmospheric pressure regulation mouth all is equipped with the silica gel shock insulator.
Preferably, the light channel faces the leaf chamber from bottom to bottom, and the air inlet and the air pressure adjusting port are located on two sides of the light channel.
Preferably, a support net is arranged in the middle of the leaf chamber.
Preferably, the channel where the air inlet is located and the channel where the air pressure adjusting port is located can both adopt hard glass tubes, and the tail ends of the two hard glass tubes are provided with glass tube sealing rings.
Preferably, a porous object is arranged at the contact part of the air outlet channel and the semi-permeable membrane.
Preferably, the periphery of the gas channel is provided with a fixed ring.
The invention has the beneficial effects that:
the invention discloses an isotope mass spectrum sample introduction system for researching photosynthesis gas exchange.A photosynthetic leaf chamber simulation device in a laboratory environment is designed by simulating green plant photosynthesis, and O is controlled manually2、CO2、H2O and light delivery to the green leaf and selective passage of O using a semi-permeable membrane2、CO2The formed mixed gas is purified by using a cold trap, and finally detected by using an isotope mass spectrometer, so that the carbon and oxygen isotope change tracks in the process of the gas for photosynthesis entering and exiting can be automatically and continuously monitored on line, the dynamic process of carbon assimilation in the natural state is researched, the generation and regulation mechanism of reducing power is deeply understood, the photosynthetic efficiency and the adversity adaptability are further improved, and the method is a nondestructive continuous test.
Drawings
Fig. 1 is a schematic structural diagram of an isotope mass spectrometry sample injection system for researching photosynthesis gas exchange provided in embodiment 1 of the present invention;
fig. 2 is a top view of a photosynthetic leaf chamber simulation apparatus provided in embodiment 1 of the present invention;
fig. 3 is a schematic structural diagram of a photosynthetic leaf chamber simulation apparatus provided in embodiment 1 of the present invention.
Detailed Description
Example 1
Example 1 provides an isotope mass spectrometry sample injection system for studying photosynthesis gas exchange, the structure of which is described in detail below.
Referring to fig. 1, the isotope mass spectrometry sample injection system comprises a photosynthetic leaf chamber simulation device 1, a cold trap 2 and an isotope mass spectrometer 3 which are sequentially connected by a pipeline from front to back.
Referring to fig. 2 and 3, the photosynthetic leaf chamber simulation apparatus 1 includes a temperature-controlled base 10, a light channel 11, a gas channel 12, a photosynthetic simulator 13, and a semi-permeable membrane 14. Wherein, a groove is arranged on the temperature control base 10, and the photosynthetic simulator 13 is arranged in the groove of the temperature control base 10; the semi-permeable membrane 14 is disposed between the bottom surface of the photosynthetic simulator 13 and the surface of the recess of the temperature controlled base 10. In order to ensure that gas does not leak, the upper surface and the lower surface of the photosynthetic simulator 13 are respectively provided with two photosynthetic simulator sealing rings 15. A leaf chamber 130 is arranged in the photosynthetic simulator 13. The gas channel 12 comprises a gas inlet 121, a gas pressure regulating port 122 and a gas outlet channel 123, the gas inlet 121, the gas pressure regulating port 122, the light channel 11 and the gas outlet channel 123 are all communicated with the leaf chamber 130, and the gas outlet channel 123 penetrates through the temperature control base 10.
In order to facilitate the injection of gas and maintain the sealing, the gas inlet 121 and the gas pressure regulating port 122 are provided with silicone spacers.
In order to make the light uniform and facilitate the air pressure adjustment in the air channel 12, the light channel 11 faces the leaf chamber 130 from the bottom down, and the air inlet 121 and the air pressure adjustment port 122 are located on both sides of the light channel 11.
In order to place the green leaf, a support net 16 is provided in the middle of the leaf chamber 130.
In order to ensure the contact compactness between the channel where the air inlet 121 is located and the channel where the air pressure regulating port 122 is located and the vane chamber 130, the channel where the air inlet 121 is located and the channel where the air pressure regulating port 122 is located can be made of hard glass tubes, and the tail ends of the two hard glass tubes are provided with glass tube sealing rings 17.
In order to prevent the semipermeable membrane 14 from being deformed by a pressure difference, a porous support 18 is provided at a contact portion of the gas outlet passage 123 and the semipermeable membrane 14.
Specifically, the photosynthetic simulator 13 is a metal disc made of stainless steel.
Specifically, the glass tube sealing ring 17 is a silica gel sealing ring.
In particular, the support screen 16 is a metal screen.
In particular, the gas channel 12 is an insert with a gas channel.
In order to fix the gas channel 12, the photosynthetic simulator 13 and the temperature controlled base 10 and prevent gas leakage from the leaf chamber 130, the periphery of the gas channel 12 is provided with a fixing ring 19, and preferably, the fixing ring 19 is a metal fixing ring.
The isotope mass spectrum sampling system is controlled by water cooling and temperature, and comprises the following components: temperature control base, circulating water, water pump, pipeline and water tank. The temperature control base is a metal block with a water channel reserved inside, is made of copper, aluminum or stainless steel, is in contact with the leaf chamber and absorbs heat of the leaf chamber, and is completely closed with the water channel reserved on the temperature control base, so that circulating water is prevented from leaking.
The water pump is used for pushing circulating water to flow, so that the water absorbing the heat of the leaf chamber flows out of the temperature control base, and the new low-temperature circulating water continues to absorb the heat of the leaf chamber.
The water pipe is connected with the water pump, the water cooling block and the water tank, and the circulating water circularly flows in a closed channel without leaking outside, so that the liquid cooling heat dissipation system can normally work.
The water tank is used for storing circulating water, redundant heat is released by the returned circulating water, the low-temperature circulating water flows into the pipeline again, and if the heat of the leaf chamber is small, the temperature of the circulating liquid cannot be obviously increased by using large-capacity circulating liquid stored in the water tank.
Example 2
step S1: firstly, placing photosynthetic sample (leaf) on supporting net 16, then assembling photosynthetic leaf chamber simulation device 1, introducing light from light channel 11, and receiving CO from gas inlet 121 of gas channel 122And O2；
Step S2: CO 22And H2O photosynthesis takes place in the leaf chamber 130 and O is produced2；
Step S3: in the leaf chamber 130 is composed of2、CO2And trace amount of H2O compositionThe mixed gas permeates the semipermeable membrane 14 by diffusion;
step S4: the mixed gas enters a cold trap 2, and trace moisture in the mixed gas is removed;
step S5: the mixed gas enters the isotope mass spectrometer 3.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. An isotope mass spectrum sample introduction system for researching photosynthesis gas exchange is characterized by comprising a photosynthetic leaf chamber simulation device (1), a cold trap (2) and an isotope mass spectrometer (3) which are sequentially connected by a pipeline from front to back,
the photosynthetic leaf chamber simulation device (1) comprises a temperature control base (10), a light channel (11), a gas channel (12), a photosynthetic simulator (13) and a semi-permeable membrane (14);
a groove is formed in the temperature control base (10), and the photosynthetic simulator (13) is arranged in the groove of the temperature control base (10);
the semi-permeable membrane (14) is arranged between the bottom surface of the photosynthetic simulator (13) and the surface of the groove of the temperature control base (10);
be provided with leaf room (130) in photosynthetic simulator (13), gas channel (12) include air inlet (121), atmospheric pressure regulation mouth (122) and air outlet channel (123), air inlet (121), atmospheric pressure regulation mouth (122), light channel (11) with air outlet channel (123) all with leaf room (130) are linked together, air outlet channel (123) pass temperature control base (10).
2. The isotope mass spectrometry sample introduction system according to claim 1, wherein the gas inlet (121) and the gas pressure regulation port (122) are provided with silica gel spacers.
3. The isotope mass spectrometry sample introduction system of claim 1,
the light channel (11) faces the leaf chamber (130) from bottom to bottom, and the air inlet (121) and the air pressure adjusting port (122) are located on two sides of the light channel (11).
4. The isotope mass spectrometry sample introduction system of claim 1,
the middle part of the leaf chamber (130) is provided with a supporting net (16).
5. The isotope mass spectrometry sample introduction system of claim 1,
the channel where the air inlet (121) is located and the channel where the air pressure adjusting port (122) is located can both adopt hard glass tubes, and the tail ends of the two hard glass tubes are provided with glass tube sealing rings (17).
6. The isotope mass spectrometry sample introduction system of claim 1,
and a porous object (18) is arranged at the contact part of the air outlet channel (123) and the semi-permeable membrane (14).
7. The isotope mass spectrometry sample introduction system of claim 1,
the periphery of the gas channel (12) is provided with a fixed ring (19).
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Citations (6)
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|---|---|---|---|---|
| JPH11326314A (en) * | 1998-05-08 | 1999-11-26 | Isao Nishi | Method for separating and simultaneously measuring response of respiration and photosynthesis of plant and its device |
| WO2006108183A2 (en) * | 2005-04-05 | 2006-10-12 | Corning Incorporated | Label free biosensors and cells |
| CN105973817A (en) * | 2016-05-10 | 2016-09-28 | 北京林业大学 | Device and method for determining trunk respiration and 13C thereof |
| CN108603154A (en) * | 2015-12-01 | 2018-09-28 | 阿尔伯瑞亚有限公司 | Bioreactor device and method |
| CN109081796A (en) * | 2018-09-06 | 2018-12-25 | 山东清创化工有限公司 | Photochemical syntheses vitamin D in a kind of tubular reactor2、D3Method |
| CN110160990A (en) * | 2019-05-30 | 2019-08-23 | 深圳华领气体技术有限公司 | Trace gas and its isotope real time on-line detection device |
-
2019
- 2019-11-20 CN CN201911139936.1A patent/CN110726793B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11326314A (en) * | 1998-05-08 | 1999-11-26 | Isao Nishi | Method for separating and simultaneously measuring response of respiration and photosynthesis of plant and its device |
| WO2006108183A2 (en) * | 2005-04-05 | 2006-10-12 | Corning Incorporated | Label free biosensors and cells |
| CN108603154A (en) * | 2015-12-01 | 2018-09-28 | 阿尔伯瑞亚有限公司 | Bioreactor device and method |
| CN105973817A (en) * | 2016-05-10 | 2016-09-28 | 北京林业大学 | Device and method for determining trunk respiration and 13C thereof |
| CN109081796A (en) * | 2018-09-06 | 2018-12-25 | 山东清创化工有限公司 | Photochemical syntheses vitamin D in a kind of tubular reactor2、D3Method |
| CN110160990A (en) * | 2019-05-30 | 2019-08-23 | 深圳华领气体技术有限公司 | Trace gas and its isotope real time on-line detection device |
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