CN110655429A - Water-soluble organic carbon fertilizer and preparation method thereof - Google Patents
Water-soluble organic carbon fertilizer and preparation method thereof Download PDFInfo
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention relates to the technical field of agriculture, in particular to a water-soluble organic carbon fertilizer and a preparation method thereof. The water-soluble organic carbon fertilizer consists of alpha-ketoglutaric acid fermentation liquor, major elements, trace elements, biological stimulin, an emulsifier, a thickening agent, a preservative and a pH regulator. The invention utilizes Yarrowia lipolytica to prepare alpha-ketoglutaric acid fermentation liquor by a liquid fermentation method, and the alpha-ketoglutaric acid fermentation liquor is compounded with major elements, trace elements, biological stimulin and other components, so that the obtained water-soluble organic carbon fertilizer has obvious promotion effect on the growth, development and metabolism of crops, and various nutrient substances are organically combined, mutually promoted, complementary in effect, high in fertilizer utilization rate, stable in fertilizer efficiency and obvious in yield increase, thus the water-soluble organic carbon fertilizer becomes a high-efficiency, multi-element and multifunctional compound organic carbon fertilizer with high biological activity, and is suitable for various crops and soil.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a water-soluble organic carbon fertilizer and a preparation method thereof.
Background
Carbon is the first of 17 essential nutrient elements of plants, and accounts for about 50% of dry matters of the plants, and the plants mainly absorb CO in air2However, the carbon provided by the air is only one fifth of the required amount, and the carbon is far from meeting the requirement of crops and becomes a short carbon plate.
The small molecular water-soluble organic carbon is also called organic carbon nutrition, effective carbon or biomass carbon, and the organic compound can be directly absorbed and utilized by microorganisms in plants and soil, can be used as a carbon source and an energy source required by the growth of the plants and the microorganisms, and meets the requirements of a carbon skeleton and energy required by the growth of the plants and the microorganisms. The organic fertilizer rich in water-soluble organic carbon is called organic carbon fertilizer, and refers to organic carbon compounds which have high water solubility and are easily absorbed by plants, such as micromolecular sugar, alcohol, carboxylic acid, ketone, aldehyde, amino acid and the like.
At present, organic wastes are mostly used as raw materials of common organic carbon fertilizers, and the common organic carbon fertilizers are decomposed into micromolecular water-soluble organic carbon with the grain diameter below 800 nanometers through biological fermentation or chemical decomposition, can be directly absorbed by plants and microorganisms, have quick response and are novel exquisite fertilizers for organic carbon nutrition of crops. However, the existing organic carbon fertilizer has single composition, unsatisfactory effects of increasing yield and improving quality, low fertilizer utilization rate and unstable fertilizer efficiency, and cannot completely meet the growth requirement of crops.
Disclosure of Invention
In view of the above, the present invention aims to provide a water-soluble organic carbon fertilizer and a preparation method thereof. The organic carbon fertilizer has high utilization rate, stable fertilizer efficiency and obvious yield increase.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a water-soluble organic carbon fertilizer, which consists of alpha-ketoglutaric acid fermentation liquor, major elements, trace elements, biological stimulin, an emulsifier, a thickening agent, a preservative and a pH regulator.
The invention utilizes Yarrowia lipolytica to prepare alpha-ketoglutaric acid fermentation liquor by a liquid fermentation method, and the alpha-ketoglutaric acid fermentation liquor is compounded with major elements, trace elements, biological stimulin and other components, so that the obtained water-soluble organic carbon fertilizer has obvious promotion effect on the growth, development and metabolism of crops, and various nutrient substances are organically combined, mutually promoted, complementary in effect, high in fertilizer utilization rate, stable in fertilizer efficiency and obvious in yield increase, thus the water-soluble organic carbon fertilizer becomes a high-efficiency, multi-element and multifunctional compound organic carbon fertilizer with high biological activity, and is suitable for various crops and soil.
In some embodiments, the water-soluble organic carbon fertilizer comprises the following raw materials in percentage by mass:
in the invention, the alpha-ketoglutaric acid fermentation liquor is prepared by fermenting yarrowia lipolytica through a liquid fermentation culture medium.
In some embodiments, the alpha-ketoglutarate fermentation broth is prepared by:
and (3) fermenting the yarrowia lipolytica in a liquid fermentation medium for 120 hours, filtering by using a microfiltration membrane, and trapping particles between 100 and 1000 nanometers to obtain the alpha-ketoglutaric acid fermentation broth filtrate.
In some embodiments, the yarrowia lipolytica has a collection number of CCTCC NO: m207143.
In some embodiments, the liquid fermentation medium consists of: 220g/L of glycerin 200-.
In some embodiments, the macroelements consist of urea and potassium nitrate; the trace elements consist of boric acid, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Cu and ammonium molybdate; the biological stimulin is one or more of humic acid, amino acid, seaweed extract and molasses leavening.
The thickening agent consists of xanthan gum and potassium alginate; the pH regulator is ethanolamine, and the preservative is potassium sorbate.
The molasses fermentation liquor is prepared by fermenting cane molasses, contains biochemical humic acid, amino acid, vitamins, inositol, polysaccharide and the like, can be prepared according to a common fermentation method in the field, and can also be purchased in the market.
In some embodiments, the water-soluble organic carbon fertilizer provided by the invention comprises the following components in percentage by mass: 60-80% of alpha-ketoglutaric acid fermentation liquor, 3-8% of urea, 2-6% of potassium nitrate, 0.5-0.8% of EDTA-Fe, 0.2-0.4% of EDTA-Mn, 0.2-0.6% of EDTA-Zn, 0.1-0.4% of EDTA-Cu, 0.5-1% of boric acid and 0.01-0.02% of ammonium molybdate. 10-20% of fulvic acid, 0.1% of xanthan gum, 0.05% of potassium alginate, 0.2-0.5% of fatty alcohol-polyoxyethylene ether, 0.2-0.5% of polyoxyethylene (10) oleyl ether, 0.5% of potassium sorbate and 1.33-1.43% of ethanolamine.
In some embodiments, the water-soluble organic carbon fertilizer comprises the following components in percentage by mass: 60% of alpha-ketoglutaric acid fermentation liquor, 8% of urea, 6% of potassium nitrate, 0.8% of EDTA-Fe0, 0.4% of EDTA-Mn0, 0.5% of EDTA-Zn0, 0.3% of EDTA-Cu0, 0.9% of boric acid, 0.02% of ammonium molybdate, 20% of molasses fermentation liquor, 0.1% of xanthan gum, 0.05% of potassium alginate, 0.5% of fatty alcohol-polyoxyethylene ether, 0.5% of polyoxyethylene (10) oil ether, 0.5% of potassium sorbate and 1.43% of ethanolamine.
In some embodiments, the water-soluble organic carbon fertilizer comprises the following components in percentage by mass: 70% of alpha-ketoglutaric acid fermentation liquor, 4% of urea, 5% of potassium nitrate, 0.5% of EDTA-Fe0, 0.2% of EDTA-Mn0, 0.2% of EDTA-Zn0, 0.3% of EDTA-Cu0, 0.8% of boric acid, 0.02% of ammonium molybdate, 16% of amino acid, 0.1% of xanthan gum, 0.05% of potassium alginate, 90.5% of AEO, 100.5% of O, 0.5% of potassium sorbate and 1.33% of ethanolamine.
In some embodiments, the water-soluble organic carbon fertilizer comprises the following components in percentage by mass: 80% of alpha-ketoglutaric acid fermentation liquor, 3% of urea, 2% of potassium nitrate, 0.5% of EDTA-Fe0, 0.2% of EDTA-Mn0, 0.3% of EDTA-Zn0, 0.1% of EDTA-Cu0, 0.5% of boric acid and 0.01% of ammonium molybdate. 11% of fulvic acid, 0.1% of xanthan gum, 0.05% of potassium alginate, 0.2% of fatty alcohol-polyoxyethylene ether, 0.2% of polyoxyethylene (10) oleyl ether, 0.5% of potassium sorbate and 1.34% of ethanolamine.
The invention also provides a preparation method of the water-soluble organic carbon fertilizer, which comprises the following steps:
adding an emulsifier into alpha-ketoglutaric acid fermentation liquor for emulsification, standing overnight, removing bottom sediment, taking supernate, adding a dissolved thickening agent, uniformly stirring, then sequentially adding biostimulant, and stirring until the biostimulant is completely dissolved; adding macroelements and microelements, stirring to dissolve completely, adding ethanolamine to adjust pH to 5.5-6, and adding antiseptic to dissolve completely.
The water-soluble organic carbon fertilizer provided by the invention consists of alpha-ketoglutaric acid fermentation liquor, major elements, trace elements, biological stimulin, an emulsifier, a thickening agent, a preservative and a pH regulator.
(1) The alpha-ketoglutaric acid fermentation liquor in the organic carbon fertilizer is mainly prepared by microbial fermentation, is rich in alpha-ketoglutaric acid and other byproducts such as pyruvic acid generated by microbial fermentation, is combined with various biological stimulin, has the function superposition, and effectively increases the biological activity of each component of the organic carbon fertilizer.
(2) The multiple biological stimulin are compounded with major elements and trace elements, so that the conditions of low fertilizer efficiency and unstable fertilizer efficiency of single application of alpha-ketoglutaric acid are overcome.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.
Fig. 1 shows a process flow diagram of a water-soluble organic carbon fertilizer.
Detailed Description
The invention discloses a water-soluble organic carbon fertilizer and a preparation method thereof, and a person skilled in the art can appropriately improve process parameters by referring to the content. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
The invention is further illustrated by the following examples:
example 1 preparation of a Water-soluble organic carbon Fertilizer of the invention
60% of alpha-ketoglutaric acid fermentation liquor, 8% of urea, 6% of potassium nitrate, 0.8% of EDTA-Fe, 0.4% of EDTA-Mn, 0.5% of EDTA-Zn, 0.3% of EDTA-Cu0.3%, 0.9% of boric acid and 0.02% of ammonium molybdate. 20% of molasses fermentation liquor, 0.1% of xanthan gum, 0.05% of potassium alginate, 0.5% of fatty alcohol-polyoxyethylene ether, 0.5% of polyoxyethylene (10) oil ether, 0.5% of potassium sorbate and 1.43% of ethanolamine.
The preparation method comprises the following steps:
(1) fermenting the yarrowia lipolytica liquid for 120h, passing through a tubular microfiltration membrane, trapping particles between 100 and 1000 nanometers to obtain a filtrate, adding emulsifiers of fatty alcohol polyoxyethylene ether and polyoxyethylene (10) oil ether into the filtrate for emulsification, standing overnight, taking a supernatant, removing bottom precipitates, and obtaining a filtrate containing alpha-ketoglutaric acid, pyruvic acid and other fermentation byproducts.
(2) Adding the dissolved xanthan gum and potassium alginate into the system, and stirring uniformly.
(3) Adding molasses fermentation liquor, and stirring until the molasses fermentation liquor is completely dissolved.
(4) Adding urea, potassium nitrate, boric acid, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Cu, boric acid and ammonium molybdate in sequence, and stirring until the materials are completely dissolved.
(5) Ethanolamine is added to adjust the pH to 5.5-6 while stirring.
(6) Potassium sorbate was added to complete dissolution.
The process flow diagram is shown in FIG. 1.
Example 2 preparation of a Water-soluble organic carbon Fertilizer of the invention
70% of alpha-ketoglutaric acid fermentation liquor, 4% of urea, 5% of potassium nitrate, 0.5% of EDTA-Fe, 0.2% of EDTA-Mn, 0.2% of EDTA-Zn2%, 0.3% of EDTA-Cu, 0.8% of boric acid and 0.02% of ammonium molybdate. 16% of amino acid, 0.1% of xanthan gum, 0.05% of potassium alginate, 0.5% of fatty alcohol-polyoxyethylene ether, 0.5% of polyoxyethylene (10) oil ether, 0.5% of potassium sorbate and 1.33% of ethanolamine.
The preparation method is the same as example 1.
Example 3 preparation of a Water-soluble organic carbon Fertilizer of the invention
80% of alpha-ketoglutaric acid fermentation liquor, 3% of urea, 2% of potassium nitrate, 0.5% of EDTA-Fe0.2%, 0.3% of EDTA-Mn0.2%, 0.5% of EDTA-Cu0.1%, 0.5% of boric acid and 0.01% of ammonium molybdate. 11% of fulvic acid, 0.1% of xanthan gum, 0.05% of potassium alginate, 0.2% of fatty alcohol-polyoxyethylene ether, 0.2% of polyoxyethylene (10) oleyl ether, 0.5% of potassium sorbate and 1.34% of ethanolamine.
The preparation method is the same as example 1.
Example 4
Alpha-ketoglutaric acid (a commercially available analytical reagent) is used for replacing alpha-ketoglutaric acid fermentation liquor in example 1, the total organic acid concentration and the use amount of other components are the same as those in example 1, and the alpha-ketoglutaric acid organic carbon fertilizer is prepared, and the application effects of the alpha-ketoglutaric acid fermentation liquor organic carbon fertilizer and the alpha-ketoglutaric acid organic carbon fertilizer in example 1 are compared. And (3) carrying out a spraying test on the water spinach, wherein the spraying dilution times are the same, water mist is uniformly and slowly sprayed on the leaf surfaces of the crops when the water spinach is sprayed, and the water spray per mu is calculated according to 100 kilograms. Spraying every time, 3 cells are treated every time at intervals of 7 days, sampling and yield measuring are carried out 8 days after spraying, the sampling is cut off at random and the root is trimmed every time, and the experimental treatment and results are shown in table 1.
TABLE 1 Effect of different treatments on swamp cabbage
And (4) conclusion: the organic carbon fertilizer effect of the fermentation liquor is optimal, and the yield is increased by 32.8 percent compared with the fresh weight of CK after the organic carbon fertilizer is sprayed for 3 times; and the alpha-ketoglutaric acid organic carbon fertilizer is diluted by the same times, the fresh weight of the alpha-ketoglutaric acid organic carbon fertilizer is increased by 7.5 percent compared with CK for the same times, and the effect is not as good as that of the fermentation broth organic carbon fertilizer. The result shows that the organic carbon fertilizer effect of the fermentation liquor is obviously better than that of the alpha-ketoglutaric acid organic carbon fertilizer under the same conditions.
Example 5
The test method comprises the following steps: a plot experiment is adopted, the test object is asparagus lettuce, and the treatment is shown in table 2. Each treated 3 cells, 20 strains per cell. 50kg of compound fertilizer (N: P: K ═ 15: 15: 15) is applied to each mu of the asparagus lettuce before transplanting as base fertilizer, the asparagus lettuce is diluted by the water-soluble organic carbon fertilizer in the embodiment 1 after being planted and survived and then is irrigated into roots, the root irrigation interval is 10 days each time, 3 times in total, and clear water is used as a contrast. And measuring indexes such as plant height, plant weight and the like of the asparagus lettuce during harvesting.
Table 2 effect of different treatments on lettuce
Note: different lower case letters indicate significant differences at different concentrations treatment on the same line, P < 0.05.
As can be seen from Table 2, the plant height, the plant weight and the yield per mu after the water-soluble organic carbon fertilizer treatment are higher than those of the non-fertilization control, and the difference is obvious. With the increase of the application concentration of the organic carbon fertilizer, the plant height, the plant weight and the conversion yield of the asparagus lettuce are gradually increased, the plant height is the highest and the yield is increased to the maximum by applying 300 times of the diluent of the organic water soluble fertilizer, the plant height and the yield are respectively 28.86cm and 48.4%, and the yield increasing effect is the most ideal.
Example 6
The test method comprises the following steps: the vegetable is used as a test crop, and a plot test is adopted. Each treated 3 cells, 20 strains per cell. 60kg of compound fertilizer (N: P: K is 15: 15) is applied to each mu of land before transplanting as base fertilizer, after field planting and survival, the water-soluble organic carbon fertilizer of the embodiment 2 is used for foliage spraying, water spraying is carried out for each mu according to 150 kg, the treatment is as shown in the table 3, the spraying interval is 15 days every time, 3 times in total, and clear water is used as a contrast. When the compound fertilizer is sprayed, the compound fertilizer is uniformly sprayed, and the SPAD value is measured after 24 hours of spraying on the premise that liquid drops do not fall off, and the weight of a single plant is measured when the compound fertilizer is harvested.
TABLE 3 Effect of different treatments on vegetables
Note: different lower case letters indicate significant differences (P <0.05) at different concentrations treatment on the same line.
As can be seen from Table 3, compared with a control, the SPAD value and the single plant weight of the crops can be remarkably increased by spraying the organic carbon fertilizer, and when the organic carbon fertilizer is diluted by 700 times, the SPAD and the yield are increased most remarkably.
Example 7
The test method comprises the following steps: celery is used as a test crop, and 30 plants are treated by adopting a pot experiment. After the transplanting survives, each treatment irrigation is applied with 19-19-19 water soluble fertilizers with the same amount, the water soluble organic carbon fertilizer in the embodiment 3 is diluted with water and irrigated with roots in the growth period, the root irrigation interval is 15 days each time, 3 times in total, and clear water is used as a control. The SPAD value was measured 24 hours after root irrigation, and the individual plant weight, plant height, and stem thickness were measured at harvest time. The respective treatment methods and results are shown in Table 4.
Table 4 effect of different treatments on celery
Note: different lower case letters indicate significant differences (P <0.05) at different concentrations treatment on the same line.
As can be seen from Table 4, compared with the control, the SPAD value, the single plant weight, the stem thickness and the plant height of celery can be increased by spraying the organic water-soluble fertilizer, and the yield increasing effect is the best when the organic water-soluble fertilizer is diluted by 500 times and used by 600 times.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The water soluble organic carbon fertilizer is characterized by comprising alpha-ketoglutaric acid fermentation liquor, major elements, trace elements, biological stimulin, an emulsifier, a thickening agent, a preservative and a pH regulator.
2. The water-soluble organic carbon fertilizer as claimed in claim 1, wherein the water-soluble organic carbon fertilizer comprises the following raw materials in percentage by mass:
3. the water-soluble organic carbon fertilizer according to claim 1, wherein the α -ketoglutarate fermentation broth is obtained by fermenting yarrowia lipolytica in a liquid fermentation medium.
4. The water-soluble organic carbon fertilizer according to claim 3, wherein the preservation number of Yarrowia lipolytica WSH-Z06 is CCTCC NO: m207143.
5. The water-soluble organic carbon fertilizer according to claim 1, wherein the α -ketoglutaric acid fermentation broth is prepared by:
and (3) fermenting the yarrowia lipolytica in a liquid fermentation medium for 120 hours, filtering by using a microfiltration membrane, and trapping particles between 100 and 1000 nanometers to obtain the alpha-ketoglutaric acid fermentation broth filtrate.
6. The water-soluble organic carbon fertilizer according to claim 5, wherein the liquid fermentation medium consists of: 220g/L of glycerin 200-.
7. The water soluble organic carbon fertilizer according to claim 1, wherein said macro-elements consist of urea and potassium nitrate; the trace elements consist of boric acid, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Cu and ammonium molybdate; the biological stimulin is one or more of humic acid, amino acid, seaweed extract and molasses fermentation liquor.
8. The water soluble organic carbon fertilizer according to claim 1, wherein said thickener consists of xanthan gum and potassium alginate; the pH regulator is ethanolamine, and the preservative is potassium sorbate.
9. The water-soluble organic carbon fertilizer as claimed in claim 2, which is characterized by comprising the following components in percentage by mass: 60-80% of alpha-ketoglutaric acid fermentation liquor, 3-8% of urea, 2-6% of potassium nitrate, 0.5-0.8% of EDTA-Fe, 0.2-0.4% of EDTA-Mn0, 0.2-0.6% of EDTA-Zn, 0.1-0.4% of EDTA-Cu, 0.5-1% of boric acid, 0.01-0.02% of ammonium molybdate, 10-20% of biostimulant, 0.1% of xanthan gum, 0.05% of potassium alginate, 0.2-0.5% of fatty alcohol polyoxyethylene ether, 0.2-0.5% of polyoxyethylene (10) oil ether, 0.5% of potassium sorbate and 1.33-1.43% of ethanolamine.
10. The preparation method of the water-soluble organic carbon fertilizer as claimed in any one of claims 1 to 9, characterized by comprising the following steps:
adding an emulsifier into alpha-ketoglutaric acid fermentation liquor for emulsification, standing overnight, removing bottom sediment, taking supernate, adding a dissolved thickening agent, uniformly stirring, then sequentially adding biostimulant, and stirring until the biostimulant is completely dissolved; adding macroelements and microelements, stirring to dissolve completely, adding ethanolamine to adjust pH to 5.5-6, and adding antiseptic to dissolve completely.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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