CN110596409B - Reagent pack applied to rapid instant detection and detection equipment thereof - Google Patents
Reagent pack applied to rapid instant detection and detection equipment thereof Download PDFInfo
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- CN110596409B CN110596409B CN201910759722.8A CN201910759722A CN110596409B CN 110596409 B CN110596409 B CN 110596409B CN 201910759722 A CN201910759722 A CN 201910759722A CN 110596409 B CN110596409 B CN 110596409B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
Abstract
The embodiment of the invention discloses a reagent pack applied to rapid instant detection and detection equipment thereof. The detection apparatus includes: the device comprises a device shell, a reagent pack and a reagent pack clamping seat, wherein the reagent box clamping seat is borne by a bottom plate and is used for accommodating the reagent pack clamping seat, and a heating device is arranged on the bottom plate and is used for heating the detection device to a set temperature; the driving device is connected with the reagent box seat and is used for driving the reagent box seat to move so as to drive the reagent and the sample in the reagent pack to perform specific binding reaction in a set step; the turbidimetric detection device is arranged at a position opposite to the reaction area of the reagent pack and is used for detecting the concentration of suspended substances of the specific binding reaction. The detection equipment has the advantages of simple operation, high automation degree, high test speed (2-5 minutes), good test precision, good repeatability and the like.
Description
Technical Field
The invention relates to the technical field of biochemical detection, in particular to a reagent pack applied to rapid and instant detection and detection equipment thereof.
Background
Due to the hardware conditions of the primary medical institution, the practical level of doctors and the limitation of outpatient times, the method is not suitable for adopting large-scale equipment (the purchase cost of the large-scale equipment is high, the operation and maintenance cost is high, and the operation is complex).
With the need of rapid development of emergency medical science, medical staff are required to utilize the most advanced facilities and methods, save lives of critical patients as much as possible and relieve disabilities of the patients to the maximum extent by the fastest and most effective means.
Therefore, simple, rapid and accurate detection is becoming a development trend in clinical examination. The small-sized intelligent instrument is more and more popular among medical workers and people due to the small-sized intelligent instrument, the portable instrument is easy to operate, and great challenges are brought to medical designers.
In the process of implementing the invention, the inventor finds that the following problems exist in the related art: most of the existing instant detection products are based on colloidal gold and fluorescence immunoassay. The above methodology has the disadvantages of complicated test operation, professional examination physician operation, and the like.
Particularly, the test precision and the repeatability are poor (CV is more than or equal to 15-30%), only semi-quantitative and accurate qualitative products are achieved, the specificity is poor, the sensitivity is low, false positive is easy to occur, the method cannot be used as clinical final diagnosis data, the method can be used as a preliminary screening method, and the current clinical requirements cannot be met.
In addition, the cost of the full-automatic biochemical analyzer is high, the starting operation cost is high, the flat test cost is very high for the basic medical treatment with the sample amount not very large, the internal structure of the analyzer has quite complicated liquid paths, light paths and movement mechanisms, and the device maintenance also has great challenges.
Disclosure of Invention
In view of the above technical problems, embodiments of the present invention provide a reagent pack for rapid and immediate detection and a detection apparatus thereof, so as to solve one or more problems of the existing biochemical analyzers.
A first aspect of embodiments of the present invention provides a reagent pack. Wherein the kit comprises: the reagent pack comprises a reagent pack body, wherein a plurality of partitions are arranged in the reagent pack body, and a reagent temporary storage area, a sample mixing area and a reaction area are formed in the reagent pack body in a divided mode; a plurality of reagent compartments, the reagent compartments being disposed within the reagent pack body, each reagent compartment enclosing a reagent; the sampling device is arranged on one side of the reagent pack body and is used for collecting a sample to be detected; the trigger device is arranged at the bottom of the reagent bin and used for destroying the sealing structure of the reagent bin so as to enable the reagent packaged in the reagent bin to flow out; and the reagent and the sample flowing out of the reagent bin enter the reagent temporary storage area, the sample mixing area and the reaction area according to a set sequence through the rotation and the vibration of the reagent pack body so as to finish the specific binding reaction of the sample.
Optionally, the partition comprises: the first partition is an arc-shaped interval extending inwards along a set radian from the side wall of the reagent pack body and forms the sample mixing area with the side wall of the reagent pack; one end of the second partition is connected with the first partition, and the second partition and the first partition form the reagent temporary storage area; the other end of the second partition is provided with a downward recess which is positioned at the bottom of the reagent pack body to form the reaction zone; the third partition comprises a partition body and an arc extension part extending outwards from the partition body; a gap is formed between the tail end of the partition body and the second partition; the tail end of the arc extension part extends to the position above the first partition so as to form a communication passage.
Optionally, the reagent cartridge comprises a first reagent cartridge, a second reagent cartridge and a third reagent cartridge for packaging the first reagent, the second reagent and the third reagent, respectively; the first reagent bin and the second reagent bin are arranged on one side of the partition body and are positioned above the sample mixing area, so that the first reagent and the second reagent enter the sample mixing area after the packaging structure is damaged;
the third reagent bin is arranged on the other side of the third partition, so that the third reagent enters the reagent temporary storage area through a gap between the third partition and the second partition after the packaging structure is damaged.
Optionally, the sampling device is a quantitative blood taking needle, which is arranged at one side close to the first reagent chamber and the second reagent chamber and is used for injecting the sample into the sample mixing area; the trigger device is a puncture needle and is respectively and correspondingly arranged at the bottoms of the first reagent bin, the second reagent bin and the third reagent bin.
A second aspect of an embodiment of the present invention provides a detection apparatus. Wherein the detection device comprises:
the equipment shell is surrounded by a bottom plate and an outer shell, and an accommodating space is formed inside the equipment shell; a kit as described above; the reagent pack clamping seat is provided with a structure matched with a reagent pack, and the reagent pack is arranged on the reagent pack clamping seat; a reagent cartridge receptacle carried by the base plate for receiving the reagent pack holder; the heating device is arranged on the bottom plate and used for heating the detection equipment to a set temperature; the driving device is connected with the reagent box seat and used for driving the reagent box seat to move so as to drive the reagent and the sample in the reagent pack to perform specific binding reaction in set steps; and the turbidimetric detection device is arranged at a position opposite to the reaction area of the reagent pack and is used for detecting the concentration of the suspended substances of the specific binding reaction.
Optionally, the system further comprises a barcode scanning device, wherein the scanning device comprises a barcode scanner and a scanning auxiliary lamp; the equipment shell is provided with a scanning window, and the bar code scanner is arranged in the scanning window and used for scanning bar codes through the scanning window; the scanning auxiliary lamp is arranged on one side of the bar code scanner and used for improving the ambient brightness during scanning.
Optionally, the turbidimetric detection means comprises a laser emitter, a scattering turbidimetric receiving sensor and a transmission turbidimetric receiving sensor; the laser emitter is arranged on one side of the reagent pack, is opposite to the reaction area of the reagent pack, and is used for emitting optical signals with set intensity and wavelength; the scattering turbidimetric receiving sensor and the transmission turbidimetric receiving sensor are arranged on the other side of the reagent pack and are used for receiving scattered light signals and transmitted light signals respectively.
Optionally, the turbidimetric detection apparatus further comprises a signal acquisition amplification board; the scattering turbidimetric receiving sensor and the transmission turbidimetric receiving sensor are arranged on the signal acquisition and amplification plate and are used for acquiring scattered light signals and transmission light signals which are converged and amplified by the signal acquisition and amplification plate.
Optionally, the mobile terminal further comprises a serial port communication interface and a touch display screen; the touch display screen is arranged on the front face of the equipment shell, and the serial port communication interface is arranged on the back face of the equipment shell and used for being in communication connection with an external printer.
Optionally, the driving device includes a stepping motor, a positioning optocoupler and a cassette fixing lock; the positioning optical coupler is used for detecting the position information of the reagent pack clamping seat, and the stepping motor is used for driving the reagent pack clamping seat to rotate and shake; the clamping seat fixing lock is used for fixing the position of the reagent pack clamping seat after the reagent pack clamping seat moves in place.
In the technical scheme provided by the embodiment of the invention, the portable detection equipment which is small in structure, does not need a specific place, can be applied to point-of-care testing (POCT), and is suitable for tens of clinical tests of proteins and biochemical tests.
The detection equipment has the advantages of simple operation, high automation degree, high testing speed (2-5 minutes), good testing precision and repeatability and the like, effectively solves a series of troubles of single POCT instrument project, complex testing operation steps, high requirement on operators, poor accuracy and the like required by primary medical treatment, and plays a vital role in accurately judging the condition of an illness of a clinician.
Drawings
FIG. 1 is a schematic diagram of an embodiment of detecting device protection cover closure according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an embodiment of detecting the opening of a protective cover of a device according to the present invention;
FIG. 3 is a schematic diagram of an embodiment of a first viewing angle of an internal structure of a detection apparatus according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of an embodiment of a second perspective of the internal structure of the inspection apparatus according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of an embodiment of a third viewing angle of the internal structure of the inspection apparatus according to the embodiment of the present invention;
FIG. 6 is a schematic diagram of one embodiment of a reagent pack and a reagent pack cartridge according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of an embodiment of the internal structure of the reagent pack according to the embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may be present. As used in this specification, the terms "vertical," "horizontal," "left," "right," "up," "down," "inner," "outer," "bottom," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the invention and for simplicity in description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items. In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Fig. 1 and fig. 2 are schematic structural diagrams of a detection apparatus provided in an embodiment of the present invention. Fig. 3 is a schematic diagram of an internal structure of the detection apparatus according to the embodiment of the present invention.
As shown in fig. 1 to 3, the external structure of the inspection apparatus is defined by a substantially trapezoidal apparatus housing 11 and a bottom plate 16 at the bottom.
The device shell 11 is provided with a plurality of interaction devices for interacting with a user, receiving a user operation instruction and feeding back corresponding operation information to the user. As shown in fig. 1 and 2, the interactive device may be a touch screen display 12 disposed on the front side of the device housing.
Still be provided with protective cover 13 that can overturn on the equipment shell, protective cover 13 sets up equipment shell's top is used for the protection to shelter from the reagent package that is located equipment shell etc..
Referring to fig. 2, the device housing 11 is further provided with a barcode scanning window 14. The bar code scanning window 13 is provided with a corresponding bar code scanning device to scan and confirm the bar code on the reagent pack.
Specifically, as shown in fig. 3, the scanning device may include a barcode scanner 141 and a scanning auxiliary lamp 142. And the bar code scanner scans the bar code through the scanning window. The scanning auxiliary lamp is arranged on one side of the bar code scanner and used for improving the brightness of the bar code during scanning.
The top of the device housing 11, which is covered by the protective cover 13, is further provided with an opening 15 for accommodating a reagent pack and a reagent pack holder. The reagent pack and the reagent pack holder can be placed at the opening.
As shown in fig. 3 to 5, the detection apparatus includes: a power interface 21, a main control system 22, a reagent pack 23, a reagent pack holder 24, a reagent cartridge holder 25, a heating device 26, a driving device 27, and a turbidimetric detection device 28.
The power interface 21 may be disposed at a tail portion of the outer housing, and connected to a suitable dc voltage source, so as to supply power to the detection device. Specifically, the dc voltage source may be converted from a mains power supply by a power adapter.
Of course, in some embodiments, the power supply may also be built in, and the portability of the device may be further improved by using a power supply manner similar to a lithium ion battery.
In other embodiments, besides the power interface, the detection device may further be provided with other peripheral interfaces to meet the use requirement of communication interaction. Such as serial communication interface 29 shown in fig. 3. The serial communication interface 29 can be connected to an external printer to print out the result.
The main control system 22 is the control core of the entire apparatus. Which may be implemented using any suitable type of microprocessor having logical computing capabilities. The touch screen display device can be electrically connected with a plurality of functional modules such as a display touch screen and a driving device, and the functional modules are controlled to run in a coordinated mode to complete an instant detection task according to a received user instruction.
The reagent pack 23 and the reagent pack holder 24 are matched for use, and the immunoreaction of the reagent pack 23 fixed between the reagent and the sample through the reagent pack holder 24 is carried out in the reagent pack 23.
Fig. 6 is a schematic structural diagram of a reagent pack and a reagent pack holder according to an embodiment of the present invention. FIG. 7 is a cross-sectional view of FIG. 6 to show the fluid paths and structures inside the reagent pack.
As shown in fig. 6 and 7, the reagent pack holder 24 covers the reagent pack 23. The reagent pack comprises a reagent pack body 231, a plurality of reagent bins 232, a sampling device 233 and a triggering device 234.
A plurality of partitions are arranged in the reagent pack body 231, and a reagent temporary storage area a, a sample mixing area B and a reaction area C are formed in the reagent pack body.
In some embodiments, the reagent pack body 231 may further include a reagent pack base 238, and the reagent pack base 238 is provided with a corresponding retaining structure 238a for retaining the position of the reagent pack.
The three different areas are communicated with each other through liquid paths, so that the reagent and the sample flowing out of the reagent bin 232 can sequentially enter the corresponding reagent temporary storage area, the sample mixing area and the reaction area through the rotation action of the reagent pack body to complete the immune reaction of the sample.
The reagent bins 232 are arranged at the top end of the reagent pack body, and each reagent bin is pre-packaged with a reagent. The type of reagent specifically packaged in the reagent chamber can be determined according to the target detection object or the detection sample which needs to be detected actually.
The sampling device 233 is arranged on one side of the reagent pack body and is used for collecting a sample to be detected. The sampling device 233 may specifically employ any suitable type of quantitative sampling structure. Specifically, it may be a quantitative blood collection needle, which automatically and quantitatively collects a sample of peripheral whole blood, serum, or venous blood.
The trigger 234 is disposed at the bottom of the reagent cartridge and is used for breaking the sealing structure of the reagent cartridge to make the reagent sealed in the reagent cartridge flow out. The particular trigger 234 selected may be adapted to the sealing structure of the reagent cartridge. In some embodiments, a puncture needle may be optionally used as a trigger for breaking the seal.
Continuing with fig. 7, in response to a typical immune response procedure, the partitions may include a first partition 235, a second partition 236, and a third partition 237.
The first partition 235 is an extension part extending along a set radian, and forms the sample mixing area a with the sidewall of the reagent pack base.
The second partition 236 is a continuous sidewall having a plurality of arcs, one end of which is connected to the extension of the first partition, and forms the reagent buffer B with the first partition.
The other end of the second partition 236 is provided with a downward recess. The recess extends to the bottom of the reagent pack body to serve as the reaction area C.
The third partition 237 includes a partition body and a circular arc extension extending outward from the partition body.
A gap is formed between the end of the partition body and the second partition 236. The reagent on the left side of the partition body can enter the reagent temporary storage area B through the gap. The arc extension part extends to the upper part of the first partition and is overlapped with at least one part of the first partition to form a communication passage between the sample mixing area and the reaction area.
Correspondingly, the reagent bin 232 is arranged at the top of the reagent pack body, and a sealing ring 239 can be further arranged above the reagent bin to ensure the sealing performance. The reagent cartridge may include a first reagent cartridge 232a, a second reagent cartridge 232b, and a third reagent cartridge 232 c. The three reagent bins are used for packaging a first reagent, a second reagent and a third reagent respectively.
The first reagent chamber 232a and the second reagent chamber 232b are disposed on one side of the third partition 237, and are located above the sample mixing area a. The third reagent chamber 232c is disposed on the other side of the third partition 237.
The bottom of each reagent bin is provided with a corresponding puncture needle for destroying the sealing structure of the reagent bin so that the reagent in the reagent bin flows out under the action of gravity.
The following describes the moving process of the reagent and the sample in the reagent pack in detail with reference to the flow sequence of the liquid path shown in fig. 7.
First, after the package structure is broken, the sample collected by the quantitative lancet located on the right side, the first reagent, and the second reagent are mixed and preheated in the sample mixing area a. The third reagent also enters the reagent buffer zone B from the gap along the liquid path L1 shown in fig. 5.
Then, by rotating the reagent pack, the direction of the reagent pack is changed, and the reagent and sample mixture in the sample mixing zone A is moved along the liquid path L2 shown in FIG. 7 into the reaction zone C. In addition, the third reagent in the reagent buffer zone similarly enters the reaction zone C through the gap along the liquid path L3 shown in FIG. 5, and performs an immunoreaction.
Referring to fig. 6, the reagent pack holder 24 is provided with a corresponding through hole at the position of the reaction region C for turbidimetric detection.
The reagent box holder 23 is disposed on the bottom plate, has a structure matched with the reagent pack holder and the reagent pack, and is used for accommodating the reagent pack holder into which the reagent pack is inserted.
The heating device 26 is disposed at the reagent cartridge seat for heating to a set temperature. The heating device 26 may be any suitable type of heat generator, including but not limited to an electric heater or the like.
The heating device 26 is connected to the main control system 22 and can maintain a constant temperature within the apparatus under the control of the main control system 22.
The driving device 27 is connected to the reagent box holder 23, and is configured to drive the reagent box holder to move so as to drive the reagent and the sample in the reagent pack to perform an immune reaction in a set step. The drive means 27 may employ any suitable type of power mechanism.
Specifically, the driving device 27 may include a stepping motor 271 and a positioning optical coupler 272. The stepping motor 271 is a power output source and can drive the reagent box seat to rotate and shake. The positioning optical coupler 272 can be arranged at a proper position of the rack and used as a position detection sensor of the reagent box seat.
The main control system 22 is electrically connected to the stepper motor 271 and the positioning optical coupler 272, and determines the position information of the reagent cartridge holder based on the detection signal of the positioning optical coupler 272, and controls the stepper motor to execute corresponding actions.
In some embodiments, the driving device may further include a cartridge fixing lock 273. The cartridge retaining lock 273 is fixedly mounted on the support base 274 and extends to engage and retain the position of the cartridge of the reagent pack when activated, ensuring that the position of the reaction chamber remains fixed during the specific binding reaction.
The turbidimetric detection device 28 is provided at a position corresponding to the reaction chamber of the reagent pack, and can detect the concentration of the suspended substance after the immunoreaction by means of turbidimetric detection.
In a preferred embodiment, the turbidimetric detection apparatus 28 may include: a laser emitter 281, a nephelometric scattering receiver sensor 282, and a nephelometric transmission receiver sensor 283.
The laser emitter 281 is arranged on one side of the reagent pack, facing the reaction area of the reagent pack, and is used for emitting an optical signal with a set intensity.
The nephelometric scattering and transmission sensor 282 and 283 are disposed on the other side of the kit for receiving scattered light and transmitted light signals, respectively.
Nephelometry and turbidimetry are two commonly used turbidimetry methods, which have different application characteristics, each with advantages. In the embodiment, the scattering turbidimetry and the transmission turbidimetry are combined, so that the advantages of high sensitivity, wide linear range and stable result can be obtained, and the method can be well applied to items measured by a specific protein meter (especially a rapid point-of-care testing (POCT) system of a biochemical meter).
In other embodiments, the turbidimetric detection apparatus 28 may further include a signal acquisition amplification board 284.
Wherein, the signal collecting and amplifying plate 284 is also arranged at the other side of the reagent pack. The nephelometric scattering reception sensor 282 and the nephelometric transmission reception sensor 283 are disposed on the signal acquisition amplification board 284.
The signal collecting and amplifying board 284 is used for collecting and amplifying the scattered light signal and the transmitted light signal, so that the nephelometric scattering and receiving sensor 282 and the nephelometric transmission and receiving sensor 283 can better collect the transmitted and scattered light signals.
The specific working process of the detection device provided by the embodiment of the present invention is now described in detail with reference to fig. 1 to 7:
after the power switch 30 of the device is turned on, the main control system 22 controls the heating device 26 to heat to a set temperature and maintain the constant temperature.
The protective cover 13 is opened, the reagent pack 23 with the packaged reagent is taken out from the reagent pack holder 24, and the taken-out reagent pack holder 24 is placed on the reagent box holder 25. The reagent pack 23 is used for automatically and quantitatively collecting a blood sample through a quantitative blood collection needle.
And after the reagent pack 23 after sampling is put into the reagent pack clamping seat 24 again, the fastening protective cover 13 is turned over. After the protective cover 13 is closed, the scanning auxiliary lamp 132 in the scanning device is turned on to provide sufficient brightness to allow the barcode scanner 131 to scan and identify the barcodes on the reagent packs.
The bar code on the reagent pack is a specific mark, and the main control system can collect a series of related information such as reagent type, expiration date, calibration curve, batch number information and the like by scanning and identifying the bar code, and automatically call a corresponding test program according to the related information obtained by collection.
The puncture needle 234 can puncture the sealing aluminum film of each reagent chamber to make the reagent sealed in the reagent chamber flow down vertically. Meanwhile, the stepping motor 271 drives the reagent pack to shake left and right at a high speed in the vertical direction, so that the reagent in the reagent bin flows out under the action of gravity.
Then, the stepping motor 271 drives the reagent tank 23 to rotate 90 ° clockwise with the positioning photocoupler 272 as a base point. Thereby, the first and second reagents of the second and third reagent compartments are caused to flow into the sample mixing zone.
At this time, the stepping motor 271 is continuously driven to perform the leftward and rightward shaking, and the reagent A, B and the quantitative blood sample are hemolyzed, mixed, and preheated.
After the mixing and preheating are finished, the stepping motor 271 drives the reagent box seat 15 to rotate counterclockwise to zero, and then the reagent box seat continues to rotate counterclockwise by about 100 degrees. After the reagent pack rotates to the position, the stepping motor 271 continuously shakes left and right, so that the reagents in the sample mixing area and the reagent temporary storage area and the liquid after mixing and preheating all enter the reaction area.
After the sample and all reagents enter the reaction zone, the stepping motor 271 drives the reagent box seat 15 back to the zero position again, and meanwhile, the clamping seat fixing lock is started to fix the position of the reaction bin.
The immunological binding reaction is initiated after the reagents and sample have all entered the reaction zone. The laser emitter 281 emits a light signal with a specific wavelength, which is irradiated into the reaction chamber.
During the immune reaction, the transmitted and scattered light signals are amplified and collected by the signal receiving amplification plate 284, and the transmitted light signals and the scattered light signals are received by the transmission receiving sensor 283 and the scattering turbidimetric receiving sensor 282, respectively, and are converted into corresponding electrical signals.
The converted electric signal is sent to an AD converter for operation processing. The test data result obtained after the operation processing can be displayed on the touch display screen 12, and can also be printed by an external printer through the serial communication interface 29. The above analog-to-digital conversion and arithmetic processing can be performed in the main control system 22.
It should be noted that, depending on the actual test items to be performed, different wavelength selection and reception modes may be used. For example, some test items may require the use of turbidimetry for transmission, some items may require turbidimetry for scattering, and some may achieve more accurate technical results through comprehensive calculations of scattered and transmitted light signals.
In summary, the detection device provided by the embodiment of the invention changes the traditional detection methods of colloidal gold and fluorescence immunity, and combines the nephelometry and the turbidimetry to detect specific items, so that the detection device has the characteristics of high sensitivity, wide linear range and stable result.
The kit can be well applied to a rapid instant detection (POCT) system of a biochemical apparatus, and can meet the requirements of dozens of protein and biochemical clinical tests (such as protein blood sugar, saccharification, CRP, D dimer, rheumatoid factor, cystatin, microalbumin, cardiac marker myoglobin and other biochemical tests, wherein liver function is glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, alkaline phosphatase, bilirubin, direct bilirubin, kidney function is urea nitrogen, uric acid, urea, creatinine and the like, and blood fat is direct high-density cholesterol, total cholesterol, high-density lipoprotein cholesterol and the like).
The whole equipment is simple to operate, high in automation degree, high in testing speed (only 2-5 minutes), good in testing precision and repeatability, and capable of effectively solving the problems that POCT instruments for primary medical treatment are single in project, complicated in testing operation steps, high in requirements on operators, poor in accuracy and the like.
The POCT detection method has a good application prospect, can effectively expand the number of POCT real-time detection technical items, and can greatly improve the detection quality of the POCT.
It should be understood that the technical solutions and concepts of the present invention may be equally replaced or changed by those skilled in the art, and all such changes or substitutions should fall within the protection scope of the appended claims.
Claims (5)
1. A detection device for rapid point-of-care detection, comprising: the equipment shell is surrounded by a bottom plate and an outer shell, and an accommodating space is formed inside the equipment shell;
the method comprises the following steps: a reagent pack;
the reagent pack comprises: the reagent pack comprises a reagent pack body, wherein a plurality of partitions are arranged in the reagent pack body, and a reagent temporary storage area, a sample mixing area and a reaction area are formed in the reagent pack body in a divided mode; a plurality of reagent compartments, the reagent compartments being disposed within the reagent pack body, each reagent compartment enclosing a reagent; the sampling device is arranged on one side of the reagent pack body and is used for collecting a sample to be detected; the trigger device is arranged at the bottom of the reagent bin and used for destroying the sealing structure of the reagent bin so as to enable the reagent packaged in the reagent bin to flow out; the reagent and the sample flowing out of the reagent bin enter the temporary reagent storage area, the sample mixing area and the reaction area according to a set sequence through the rotation and the vibration of the reagent pack body so as to finish the specific binding reaction of the sample;
the partition comprises:
the first partition is an arc-shaped interval extending inwards along a set radian from the side wall of the reagent pack body and forms the sample mixing area with the side wall of the reagent pack;
one end of the second partition is connected with the first partition, and the second partition and the first partition form the reagent temporary storage area;
the other end of the second partition is provided with a downward recess which is positioned at the bottom of the reagent pack body to form the reaction zone;
the third partition comprises a partition body and an arc extension part extending outwards from the partition body;
a gap is formed between the tail end of the partition body and the second partition; the tail end of the arc extension part extends to the position above the first partition so as to form a communication passage;
the reagent bin comprises a first reagent bin, a second reagent bin and a third reagent bin which are used for packaging a first reagent, a second reagent and a third reagent respectively;
the first reagent bin and the second reagent bin are arranged on one side of the partition body and are positioned above the sample mixing area, so that the first reagent and the second reagent enter the sample mixing area after the packaging structure is damaged;
the third reagent bin is arranged on the other side of the third partition, so that a third reagent enters the reagent temporary storage area through a gap between the third partition and the second partition after the packaging structure is damaged;
the sampling device is a quantitative blood taking needle, is arranged on one side close to the first reagent bin and the second reagent bin, and is used for injecting a sample into the sample mixing area; the trigger devices are puncture needles and are respectively and correspondingly arranged at the bottoms of the first reagent bin, the second reagent bin and the third reagent bin;
the reagent pack clamping seat is provided with a structure matched with a reagent pack, and the reagent pack is arranged on the reagent pack clamping seat;
a reagent cartridge receptacle carried by the base plate for receiving the reagent pack holder;
the heating device is arranged on the bottom plate, arranged at the reagent box seat and used for heating the detection equipment to a set temperature;
the driving device is connected with the reagent box seat and used for driving the reagent box seat to move so as to drive the reagent and the sample in the reagent pack to perform specific binding reaction in set steps;
a turbidimetric detection device disposed at a position opposite to the reaction zone of the reagent pack, for detecting the concentration of suspended substances of the specific binding reaction;
the driving device comprises a stepping motor, a positioning optocoupler and a clamping seat fixing lock;
the positioning optical coupler is used for detecting the position information of the reagent pack clamping seat, and the stepping motor is used for driving the reagent pack clamping seat to rotate and shake;
the clamping seat fixing lock is used for fixing the position of the reagent pack clamping seat after the reagent pack clamping seat moves in place.
2. The detection apparatus according to claim 1, further comprising a barcode scanning device, the scanning device comprising a barcode scanner and a scanning auxiliary lamp;
the equipment shell is provided with a scanning window, and the bar code scanner is arranged in the scanning window and used for scanning bar codes through the scanning window; the scanning auxiliary lamp is arranged on one side of the bar code scanner and used for improving the ambient brightness during scanning.
3. The inspection apparatus of claim 1, wherein the turbidimetric inspection device comprises a laser emitter, a scattered turbidimetric receiving sensor, and a transmitted turbidimetric receiving sensor;
the laser emitter is arranged on one side of the reagent pack, is opposite to the reaction area of the reagent pack, and is used for emitting optical signals with set intensity and wavelength;
the scattering turbidimetric receiving sensor and the transmission turbidimetric receiving sensor are arranged on the other side of the reagent pack and are used for receiving scattered light signals and transmitted light signals respectively.
4. The detection apparatus according to claim 3, wherein the turbidimetric detection device further comprises a signal acquisition amplification board;
the scattering turbidimetric receiving sensor and the transmission turbidimetric receiving sensor are arranged on the signal acquisition and amplification plate and are used for acquiring scattered light signals and transmission light signals which are converged and amplified by the signal acquisition and amplification plate.
5. The detection device according to claim 1, further comprising a serial communication interface and a touch display screen;
the touch display screen is arranged on the front face of the equipment shell, and the serial port communication interface is arranged on the back face of the equipment shell and used for being in communication connection with an external printer.
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| US4883763A (en) * | 1984-05-03 | 1989-11-28 | Abbott Laboratories | Sample processor card for centrifuge |
| US5162237A (en) * | 1988-04-11 | 1992-11-10 | Miles Inc. | Reaction cassette for preforming sequential analytical assays by noncentrifugal and noncapillary manipulations |
| US5372948A (en) * | 1993-03-17 | 1994-12-13 | Miles Inc. | Assay and reaction vessel with a compartmentalized solubilization chamber |
| WO1999060397A1 (en) * | 1998-05-18 | 1999-11-25 | University Of Washington | Liquid analysis cartridge |
| US8835184B2 (en) * | 2007-09-14 | 2014-09-16 | Biosensia Patents Limited | Analysis system |
| KR100798471B1 (en) * | 2007-10-08 | 2008-01-28 | 주식회사 인포피아 | Reaction cassette for measuring glycated hemoglobin and measuring method thereof |
| US10493448B2 (en) * | 2015-03-13 | 2019-12-03 | Bio-Rad Laboratories, Inc. | Assay cartridge |
| CN204832049U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Detect box |
| CN105013546B (en) * | 2015-07-03 | 2017-12-08 | 艾康生物技术(杭州)有限公司 | Biological sample reaction box |
| EP3438645B1 (en) * | 2016-03-30 | 2022-12-21 | Osang Healthcare Co., Ltd. | Check cassette, measurement device, system for compensating for light intensity of light source for measurement device, method for compensating for light intensity of light source for measurement device, and recording medium |
| GB201619509D0 (en) * | 2016-11-18 | 2017-01-04 | Univ Court Of The Univ Of St Andrews The | Sample detection device |
| CN207396345U (en) * | 2017-03-21 | 2018-05-22 | 广州埃克森生物科技有限公司 | A kind of POCT specific proteins detection analysis instrument |
| CN108732134A (en) * | 2018-07-18 | 2018-11-02 | 上海原科实业发展有限公司 | A kind of scattering of multi-wavelength and transmittance opacimetry device |
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