CN110589978A - Freshwater algae domestication method and process beneficial to freshwater algae for treating sewage - Google Patents

Freshwater algae domestication method and process beneficial to freshwater algae for treating sewage Download PDF

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Publication number
CN110589978A
CN110589978A CN201910921762.8A CN201910921762A CN110589978A CN 110589978 A CN110589978 A CN 110589978A CN 201910921762 A CN201910921762 A CN 201910921762A CN 110589978 A CN110589978 A CN 110589978A
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algae
sewage
freshwater
sewage treatment
vitamin
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国玉庆
杨安凯
陈凯
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Shandong Oka Environmental Protection Engineering Co Ltd
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Shandong Oka Environmental Protection Engineering Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/24—Treatment of water, waste water, or sewage by flotation
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/30—Treatment of water, waste water, or sewage by irradiation
    • C02F1/32—Treatment of water, waste water, or sewage by irradiation with ultraviolet light
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2301/00—General aspects of water treatment
    • C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/04—Disinfection

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Hydrology & Water Resources (AREA)
  • Virology (AREA)
  • Water Supply & Treatment (AREA)
  • Biomedical Technology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a freshwater algae domestication method and a process beneficial to freshwater algae treatment sewage, which comprises the steps of collecting algae in different freshwater areas, screening the freshwater algae by setting a specific culture medium, screening algae with high growth speed and domesticating the algae; treating sewage with domesticated and cultured freshwater algae, and putting the domesticated freshwater algae into first, second and third stage sewage treatment tanks connected in series to allow sewage to flow sequentially, wherein the putting density of the freshwater algae is 0.8-1.2kg/m3、1.0‑1.5 kg/m3、0.8‑1.2 kg/m3(ii) a The invention screens and cultures the freshwater algae species, and the algae domesticates the freshwater algae after culture to N,The absorption and enrichment of P, organic pollutants, metal ions, trace elements and radioactive elements are obviously improved, and the low temperature resistance of the freshwater algae is improved. The process of the invention takes the algae treatment pool as a main body, physical and chemical pretreatment is not needed, and the treated effluent can be directly discharged.

Description

Freshwater algae domestication method and process beneficial to freshwater algae for treating sewage
Technical Field
The invention belongs to the field of algae production and sewage treatment, and particularly relates to a freshwater algae domestication method and a process beneficial to freshwater algae sewage treatment.
Background
The problem of sewage treatment is a problem generally concerned by China and even countries in the world, and finding a treatment method which is simple to operate, low in cost and good in treatment effect is a key point for solving the problem. Algae is a low-grade plant body with various forms of photosynthetic pigments, and is propagated through spores and gametes, many algae autotrophic organisms use light energy as a main energy source, and perform photosynthesis by utilizing nutrient substances such as nitrogen, carbon and the like in water to convert the nutrient substances into substances such as intracellular amino acid, protein and the like, and phosphate is absorbed by algae cells and is converted into intracellular organic matters such as ATP, phospholipid and the like through various phosphorylation pathways. Meanwhile, the algae consume carbon dioxide in the water body through photosynthesis to release oxygen, and aerobic bacteria in the water body decompose and convert organic pollutants by using the oxygen, so that the content of organic matters in the water body is reduced, and the carbonation degree balance of the water body can be adjusted.
The algae plays a complex role in sewage treatment, and can oxidize and decompose organic matters and reduce the concentration of nitrogen and phosphorus; organic pollutants, metal ions, trace elements and radioactive elements can be enriched; can be used as fertilizer, bait, food or even health product; but also as a method of detection or an index of detection. The mechanism of algae for purifying water quality is that algae supplies oxygen to water body through photosynthesis to make aerobic bacteria continuously degrade organic matter, while algae degrades carbon dioxide generated by organic matter by using bacteria, so that algae and bacteria supplement each other in the process of purifying water quality and are called algae bacteria symbiosis. The algae can not only treat the wastewater, but also remove nutrient substances such as nitrogen, phosphorus and other organic matters in the water body, and has good purification effect on the sewage in the natural water area. The algae has specificity on N, P absorption, organic pollutant treatment and metal ion absorption, and is regulated by various environmental conditions such as temperature, illumination, salinity, nutrient salt formula and the like to a certain extent, but research processes related to freshwater algae are rarely reported. The effect of various algae on sewage treatment is different, the growth cycle difference is larger, the tolerance to the polluted water source is poor, the algae can be dead in a large area when the algae is directly applied to the sewage treatment, the sewage treatment efficiency is low, and the water body can be further polluted.
Disclosure of Invention
In order to overcome the problems of short survival time of algae, poor tolerance to a polluted water source and low treatment efficiency in the prior art, the invention provides a freshwater algae domestication method and a process beneficial to freshwater algae for treating sewage.
The invention is realized by the following technical scheme:
a method for domesticating fresh water algae comprises collecting algae in different fresh water areas, screening fresh water algae by setting specific culture medium, screening out algae with high growth speed, and domesticating;
the culture medium comprises: NaNO3，NaH2PO4·H2O，NaSiO3·9H2O, trace elements and vitamins;
the trace elements include: FeCl3·6H2O，Na2EDTA2·H2O, CuSO4·5H2O， Na2MoO4·2H2O，ZnSO4·7H2O，CoCl2·6H2O，MnCl4·H2O；
The vitamins include: vitamin B1, vitamin B7 and vitamin B12;
preferably, the acclimatization culture time is 1 ~ 3 months.
PreferablyThe concentration of each component in the culture medium is as follows: NaNO3 8.82×10-4M，NaH2PO4·H2O 3.62×10-5M，NaSiO3·9H2O 1.06×10-4M， FeCl3·6H2O 1.17×10-5M，Na2EDTA2·H2O 1.17×10- 5M, CuSO4·5H2O 3.93 ×10-8M Na2MoO4·2H2O 2.60×10-8M，ZnSO4·7H2O 7.65×10-8M，CoCl2·6H2O 4.20 ×10-8M，MnCl4·H2O 9.10×10-7M, vitamin B12.96X 10-7M, vitamin B72.05X 10-9M, vitamin B123.69X 10-10M。
In the invention, freshwater algae obtained by domestication culture is used for treating sewage, and the sewage treatment process comprises the following steps: putting the domesticated freshwater algae into a first-stage, a second-stage and a third-stage sewage treatment tanks, and enabling sewage to flow through the first-stage, the second-stage and the third-stage sewage treatment tanks in sequence;
the feeding density of the fresh water algae in the first, second and third sewage pools is 0.8-1.2kg/m3、1.0-1.5 kg/m3、0.8-1.2 kg/m3；
The first, second and third stage sewage treatment tanks are connected in series.
Preferably, the horizontal flow velocity of the sewage in the first, second and third sewage treatment tanks is 0.06 m/s.
Preferably, the sewage of the primary sewage treatment is settled sewage.
Preferably, the sewage treatment is provided with a lamp strip with red and blue lamps at intervals, wherein the blue light wavelength is 400 ~ 520nm, and the red light wavelength is 610 ~ 720 nm.
Preferably, the sewage after the three-stage sewage treatment is subjected to suspended matter removal and ultraviolet disinfection treatment in an air floatation tank in sequence.
The fresh water algae is domesticated and cultured in the culture medium, the absorption and enrichment of N, P, organic pollutants, metal ions, trace elements and radioactive elements of the domesticated and cultured fresh water algae are obviously improved, the low temperature resistance of the fresh water algae is increased, and the algae still keeps high growth rate and removal rate at 7 ~ 12 ℃.
The artificial fishing filtration after the fresh water algae among the sewage treatment pond in this patent becomes yellow, and the fresh water algae that grows adherent clearance once every week, the discarded alga of clearance can be used to make fertilizer.
Through tertiary sewage treatment pond to sewage treatment, make sewage treatment's efficiency promote greatly, handle out water quality of water moreover and reach III class water quality standards of surface water.
The air floatation tank adopts pressurized dissolved air floatation, and solid-liquid separation is realized by mutual adhesion of micro bubbles, crushed algae, impurities and flocs, so that suspended matters such as micro fine algae are removed.
The disinfection tank is provided with ultraviolet disinfection equipment for further sterilization and algae killing and is used as a storage tank.
Advantageous effects
1. According to the invention, through screening and culturing algae seeds, the absorption and utilization capacity of the algae seeds on nitrogen and phosphorus is improved, the living space of wild algae is occupied, the competitive advantage is exerted to a greater extent, the absorption and enrichment of N, P, organic pollutants, metal ions, trace elements and radioactive elements by wild algae in an incoming water body and fresh water algae cultured by domestication of the domesticated algae are obviously improved, and the low temperature resistance of the fresh water algae is improved.
2. The process mainly uses the algae treatment pool as a main body, physical and chemical pretreatment is not needed, and the treated effluent can be directly discharged; the process can be used in four seasons, avoids secondary pollution and replanting problems of death of aquatic plants, rottenness of leaves and the like in winter, and can also make up the defect that the artificial floating island cannot operate in winter.
Drawings
FIG. 1 is a flow diagram of a sewage treatment process;
FIG. 2 algae treatment system
In the figure, 1, a primary sewage treatment tank 2, a secondary sewage treatment tank 3, a tertiary sewage treatment tank 4 and a lamp strip are shown, wherein the arrow direction indicates the water flow direction.
Detailed Description
In order to make the technical solutions of the present invention better understood, the following clear and complete description of the technical solutions of the present invention, with reference to the accompanying drawings, shall belong to the protection scope of the present application, based on the embodiments in the present application, other similar embodiments obtained by those of ordinary skill in the art without creative efforts.
Example 1
FIG. 1 is a flow chart of a sewage treatment process;
FIG. 2 is an algae treatment system, which includes 1, a primary wastewater treatment tank 2, a secondary wastewater treatment tank 3, a tertiary wastewater treatment tank 4, light strips (red-blue interval, blue wavelength: 400 ~ 520nm, red wavelength: 610 ~ 720 nm), and the direction of the arrows indicates the direction of water flow, and this embodiment will now be described with reference to the accompanying drawings:
(1) rinsing fresh water algae from Jinan Daling lake, Yangchun river, sewage treatment plant, Xiaoqing river, Taiping river, yellow river and Jinan wetland park with clear water, and culturing in conical flask with culture medium (NaNO: culture medium)3 8.82×10-4M，NaH2PO4·H2O 3.62×10-5M，NaSiO3·9H2O 1.06×10-4M， FeCl3·6H2O 1.17×10-5M，Na2EDTA2·H2O 1.17×10-5M, CuSO4·5H2O 3.93 ×10-8M Na2MoO4·2H2O 2.60×10-8M，ZnSO4·7H2O 7.65×10-8M，CoCl2·6H2O 4.20 ×10-8M，MnCl4·H2O 9.10×10-7M, vitamin B12.96X 10-7M, vitamin B72.05X 10-9M, vitamin B123.69X 10-10M), after three days, discovering that the growth and the propagation of the sphingomonas near a sewage treatment plant are fast, continuing to perform acclimatization culture on the sphingomonas for 1 month by using the culture medium, and after 1 month, performing acclimatization culture on the sphingomonas in 5-liter barrels, 20-liter barrels andcarrying out amplification culture in the culture pond;
(2) putting the sphingomyelina obtained by the enlarged culture in the step (1) into a first-level sewage treatment tank, a second-level sewage treatment tank and a third-level sewage treatment tank, wherein the putting densities are 1kg/m respectively3、1.2 kg/m3、1 kg kg/m3(ii) a Settling sewage to be treated in the Ming lake in a primary settling tank to remove silt and suspended particles in inlet water, introducing the settled sewage into a primary sewage treatment tank, and controlling the water inlet amount of the primary sewage treatment tank to be 437.5m3And the water flows through the second-stage sewage treatment tank and the third-stage sewage treatment tank in sequence, the sizes of single tanks of the third-stage sewage treatment tank are all 80.0m multiplied by 9.0m, the effective water depth is 0.5m, the horizontal flow velocity of the water in the first-stage, second-stage and third-stage sewage treatment tanks is controlled to be 0.06m/s, and the water flowing out of the third-stage sewage treatment tank is subjected to suspended matter removal and ultraviolet sterilization treatment in an air floatation tank. The algae in the sewage treatment tank are manually fished out and filtered after becoming yellow, and are used for producing organic fertilizer after being primarily dried. The flow chart of the sewage treatment in the example 1 is shown in fig. 1, the diagram of the algae treatment system is shown in fig. 2, and various indexes of the treated water are detected, and the results are shown in table 1:
TABLE 1 pollutant removal rate analysis chart for sewage treatment system
Note that the blue light (wavelength: 400 ~ 520 nm) and the red light (wavelength: 610 ~ 720 nm) on the sewage treatment pool are turned on at night, and the algae continue to carry out photosynthesis without affecting water treatment.
The result shows that the sewage is treated by the three-stage sewage treatment tank, so that the sewage treatment efficiency is greatly improved, the quality of the treated outlet water reaches the III-class water quality standard of the surface water, and some wild algae exist in lake water and water, and in order to ensure that the overall water quality meets the purification requirement, the system inhibits the increase of the wild blue-green algae in the water from two aspects by using the algae inhibiting mode.

Claims (8)

1. A method for domesticating fresh water algae is characterized in that the algae in different fresh water areas are collected, fresh water algae is screened by setting a specific culture medium, and algae species with high growth speed are screened and domesticated;
the culture medium comprises: NaNO3，NaH2PO4·H2O，NaSiO3·9H2O, trace elements and vitamins;
the trace elements include: FeCl3·6H2O，Na2EDTA2·H2O, CuSO4·5H2O， Na2MoO4·2H2O，ZnSO4·7H2O，CoCl2·6H2O，MnCl4·H2O；
The vitamins include: vitamin B1, vitamin B7 and vitamin B12.
2. The acclimatization method according to claim 1, wherein the acclimatization culture time is 1 ~ 3 months.
3. The acclimatization method according to claim 1, wherein the concentration of each component in the culture medium is: NaNO3 8.82×10-4M，NaH2PO4·H2O 3.62×10-5M，NaSiO3·9H2O 1.06×10-4M， FeCl3·6H2O 1.17×10-5M，Na2EDTA2·H2O 1.17×10-5M, CuSO4·5H2O 3.93 ×10-8M Na2MoO4·2H2O 2.60×10-8M，ZnSO4·7H2O 7.65×10-8M，CoCl2·6H2O 4.20 ×10-8M，MnCl4·H2O 9.10×10-7M, vitamin B12.96X 10-7M, vitamin B72.05X 10-9M, vitamin B123.69X 10-10M。
4. The process for treating sewage with fresh-water algae cultured by the acclimatization method according to any one of claims 1 ~ 3, wherein the acclimatized fresh-water algae are placed in first, second and third stage sewage treatment tanks, and the sewage is passed through the first, second and third stage sewage treatment tanks in sequence;
the feeding density of the fresh water algae in the first, second and third sewage pools is 0.8-1.2kg/m3、1.0-1.5 kg/m3、0.8-1.2 kg/m3；
The first, second and third stage sewage treatment tanks are connected in series.
5. The process of claim 4, wherein the horizontal flow rate of the wastewater in the first, second and third wastewater treatment tanks is 0.06 m/s.
6. The process of claim 4, wherein the first stage is a settling process.
7. The process of claim 4, wherein the wastewater treatment unit comprises red and blue light spaced apart strips having a blue light wavelength of 400 ~ 520nm and a red light wavelength of 610 ~ 720 nm.
8. The process of sewage treatment according to claim 4, wherein the sewage after the third stage sewage treatment is sequentially subjected to air flotation tank for removing suspended matters and ultraviolet disinfection treatment.
CN201910921762.8A 2019-09-27 2019-09-27 Freshwater algae domestication method and process beneficial to freshwater algae for treating sewage Pending CN110589978A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111943360A (en) * 2020-07-27 2020-11-17 邵盛熙 Prevention and treatment method for algal bloom disasters of eutrophic water body
CN112322499A (en) * 2020-10-29 2021-02-05 深圳市鑫承诺环保产业股份有限公司 Autotrophic heterotrophic alternative domestication cultured dunaliella salina and application thereof in treatment of marine ship domestic sewage
GB2617306A (en) * 2021-03-31 2023-10-11 Industrial Phycology Ltd Consumption of at least one compound from a fluid

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111943360A (en) * 2020-07-27 2020-11-17 邵盛熙 Prevention and treatment method for algal bloom disasters of eutrophic water body
CN111943360B (en) * 2020-07-27 2022-10-18 邵盛熙 Prevention and treatment method for algal bloom disasters of eutrophic water body
CN112322499A (en) * 2020-10-29 2021-02-05 深圳市鑫承诺环保产业股份有限公司 Autotrophic heterotrophic alternative domestication cultured dunaliella salina and application thereof in treatment of marine ship domestic sewage
GB2617306A (en) * 2021-03-31 2023-10-11 Industrial Phycology Ltd Consumption of at least one compound from a fluid

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Application publication date: 20191220