CN110579598B - Optical signal detection device, system and method - Google Patents
Optical signal detection device, system and method Download PDFInfo
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The present application provides an apparatus for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte, comprising: at least one set of first light sources, each set of first light sources comprising at least two first light sources that are individually controllable to illuminate the reaction zone under first and second illumination conditions; at least one first optical signal detector for detecting a first optical signal detection value pair of the reaction region under a first irradiation condition and a second optical signal detection value under a second irradiation condition; and an optical signal correction value determining unit for performing a predetermined process on the first optical signal detection value pair; and determining an optical signal correction value of the reaction region based on the predetermined processing result, the second optical signal detection value, and the optical signal value correlation function. By using the device, the influence of the position error of the reaction area on the accuracy of the detection result can be eliminated.
Description
Technical Field
The present application relates generally to the field of detection of specific substances and, more particularly, to methods, devices and systems for detecting optical signal values in a reaction zone of a reaction carrier after reaction with an analyte.
Background
In recent years, due to abuse of pesticides, hormones and antibiotics for a long time, the food safety problem of China is more and more serious, the food safety detection of the current mainstream is mainly completed by a special department under the food and medicine administration through large-scale liquid phase, mass spectrum or liquid-mass chromatography, and is assisted by a large-scale table gold mark detector, so that professional operators are required to complete the detection, the period of obtaining the result is long, and the method cannot be applied to on-site timely detection.
With the improvement of the living standard of people, home health self-test is more and more important for modern people, but the mainstream early pregnancy and ovulation monitoring at present adopts a visual inspection method or disposable pen type qualitative measurement, and physical indexes cannot be monitored in a home environment for a long time and quantitatively.
The test paper suitable for rapid detection and capable of being stored at normal temperature is widely used at present, and particularly, the colloidal gold test paper is low in cost, high in detection speed and capable of being produced in batch, so that the colloidal gold test paper is rapidly popularized in the food safety field and the household health self-test field.
The detection principle of the test paper for the specific substance is that a labeled reactant capable of reacting with the specific substance is arranged in a reaction area of the test paper, after a sample to be tested is added into the test paper, the specific substance in the sample to be tested gradually enters the reaction area to react with the labeled reactant, and after the reaction, optical signal changes, such as color depth changes, fluorescence intensity changes and the like, are formed in the reaction area, and the content of the specific substance can be detected by utilizing the method due to the functional relation between the optical signal changes and the concentration of the specific substance to be tested.
Aiming at the judgment of the change of the optical signal of the test paper, the current technology mainly comprises the following three modes. The first way is to use test paper with color change, and make qualitative or semi-quantitative judgment by observing the color shade of the detection line of the reaction area on the test paper by eyes. The second way is to take a picture of the color development change of the test paper using an image sensor as a detection element, and then use image recognition to make a qualitative or quantitative judgment. The third method is to use the principle of reflection of light, receive the reflected light signal using a photoelectric sensor as a photoelectric receiving device, and determine the intensity of the received light signal.
However, in the first technical solution, the test paper has great interference to human factors, and the judgment error of different people or people without experience is great, so that the test paper can only be used for qualitative or semi-quantitative judgment. In addition, manual judgment of the fluorescent test strip cannot be performed.
In the second technical scheme, the control system is complex, imaging is needed by using an optical lens, and the imaging quality directly influences the result judgment, so that a better optical device is needed to be used, and the cost is high, so that the cost advantage of the colloidal gold test paper is offset to a certain extent. In addition, the whole scheme is large in size and cannot be miniaturized.
In the third technical scheme, the design of the test paper has low implementation cost, simple structure and can be designed into a micro instrument, however, the design of the scheme has test paper background interference, and the influence of sample color interference and position errors of a reaction area (such as a color development strip) on the accuracy and stability of a detection result is great. In addition, because the limitation of test paper production technology, the position error of color development area can't eliminate completely, and food safety detection field sample colour is very different moreover, and sample colour can not effectively get rid of through test paper sample pad, and then can not eliminate the interference that sample colour brought to the measurement to make this scheme unable effective quantitative determination of realization.
Disclosure of Invention
In view of the foregoing, the present application provides a method, apparatus and system for detecting an optical signal value in a predetermined reaction zone of a reaction carrier after reaction with an analyte. By using the method, the device and the system, the influence of the background interference of the test strip, the sample color interference and/or the reaction area position error on the accuracy of the detection result can be eliminated.
According to an aspect of the present application, there is provided an apparatus for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte, comprising: at least one set of first light sources, each set of first light sources comprising at least two first light sources, the at least two first light sources being separately controllable to illuminate the same reaction zone of the reaction carrier under first illumination conditions and second illumination conditions; at least one first optical signal detector for detecting a first optical signal detection value pair of the reaction region under the first irradiation condition and a second optical signal detection value under the second irradiation condition; and an optical signal correction value determining unit configured to perform a predetermined process on the first optical signal detection value pair; and determining an optical signal correction value of the reaction region after the position error correction based on the predetermined processing result, the second optical signal detection value, and a corresponding optical signal value correlation function, wherein the optical signal value correlation function is used for reflecting a functional relationship between the optical signal correction value after the position error correction and the predetermined processing result and the second optical signal detection value, and wherein the first irradiation condition includes that two groups of light sources which are composed of the first light sources in each group of the first light sources according to a specified rule are respectively irradiated, and the second irradiation condition includes that the light sources which are related to the light sources forming the first irradiation condition are irradiated.
Optionally, in one example of the above aspect, the second irradiation condition includes irradiation by a light source group including at least a light source selected from the two groups of light sources.
Optionally, in one example of the above aspect, the second irradiation condition includes irradiation by all of the at least two first light sources.
Optionally, in one example of the above aspect, the predetermined process includes a difference process or a ratio process.
Optionally, in one example of the above aspect, the apparatus may further include a detection parameter setting unit for setting a detection start time, a detection stop time, and a detection interval period of the first optical signal detector according to properties of a specific substance to be detected and a label on the reaction carrier; a timer; and the timing triggering unit is connected with the timer and is used for triggering the timer to start timing after the reaction carrier enters the detection ready state.
Optionally, in one example of the above aspect, the first light source is a light source having a full wavelength range or a specific wavelength range.
Optionally, in one example of the above aspect, the first light source is a fluorescence excitation light source, and the first optical signal detector has a filtering characteristic matching a wavelength of the excited fluorescence.
Optionally, in one example of the above aspect, the positions of the at least two first light sources are arranged so as to be located on both sides of the reaction zone of the standard reaction carrier when the standard reaction carrier is in the detection ready state.
Optionally, in one example of the above aspect, the positions of the at least two first light sources are arranged so as to be symmetrically located on both sides of the reaction zone of the standard reaction carrier when the standard reaction carrier is in the detection ready state.
Optionally, in one example of the above aspect, the apparatus may further include: and the temperature sensor is used for detecting temperature data when the reaction carrier is in a detection state.
Optionally, in one example of the above aspect, the optical signal value correlation function is generated based on history data of a predetermined processing result, history data of a second optical signal detection value, and history data of a corresponding optical signal detection reference value; and/or the optical signal value correlation function is associated with a model of the first optical signal detector, the first and second illumination conditions.
Optionally, in one example of the above aspect, the apparatus may further include: at least one second optical signal detector for detecting a third optical signal detection value of a non-reaction region of the reaction carrier under a third irradiation condition, and the optical signal correction value determining unit further includes: the background correction module is configured to obtain the second optical signal detection value after background correction based on the second optical signal detection value, the third optical signal detection value and the corresponding irradiation conditions, and the optical signal correction value determining unit is configured to: determining an optical signal correction value of the reaction area after position error correction based on the preset processing result, the corrected second optical signal detection value and a corresponding optical signal value correlation function; or the optical signal correction value determining unit is further configured to determine, based on the determined optical signal correction value of the predetermined reaction area after the position error correction, the third optical signal detection value, and the corresponding irradiation condition, the optical signal correction value of the reaction area after the background correction.
Optionally, in one example of the above aspect, the second irradiation condition and the third irradiation condition are the same.
Optionally, in one example of the above aspect, the second irradiation condition and the third irradiation condition are provided by the same light source or by different light sources.
Optionally, in one example of the above aspect, the first optical signal detector and the second optical signal detector are implemented with the same optical signal detector.
Optionally, in one example of the above aspect, the apparatus may further include: and a specific substance content determining unit for determining the content of the specific substance based on the determined light signal correction value.
Optionally, in one example of the above aspect, the apparatus may further include: and the communication unit is used for data communication between the specific substance content determining unit and the upper computer.
Optionally, in one example of the above aspect, the specific substance content determining unit is provided in a host computer or a cloud server, and the apparatus may further include: and a communication unit for data communication between the optical signal correction value determination unit and the specific substance content determination unit.
Optionally, in one example of the above aspect, the apparatus may further include: and a controller for controlling the illumination of the respective light sources, the detection of the respective light signal detectors, the data communication between the respective components in the device and/or the data communication between the components in the device and the outside.
Optionally, in one example of the above aspect, the specific substance includes one of: toxins, antibiotics, pesticides or hormones.
According to another aspect of the present application, there is provided an apparatus for detecting an optical signal value in a reaction region of a reaction carrier after a reaction with an analyte, comprising: at least one set of first light sources, each set of first light sources being for illuminating the same reaction zone of the reaction carrier under first illumination conditions; at least one first optical signal detector for detecting a first optical signal detection value of the reaction zone under the first irradiation condition; at least one set of second light sources, each set of second light sources being for illuminating the same non-reaction zone of the reaction carrier under second illumination conditions; the device comprises at least one second optical signal detector, an optical signal correction value determining unit and a background correction value determining unit, wherein the second optical signal detector is used for detecting a second optical signal detection value of a non-reaction area of the reaction carrier under the second irradiation condition, and the optical signal correction value determining unit is used for determining the optical signal correction value of the reaction area after background correction based on the first optical signal detection value, the second optical signal detection value and the corresponding irradiation condition.
Optionally, in one example of the above aspect, the at least one set of first light sources and the at least one set of second light sources are implemented with the same light source, and the same light source is arranged as an area between the at least one first light signal detector and the at least one second light signal detector.
Optionally, in one example of the above aspect, the positions of the at least one set of first light sources and the at least one set of second light sources are arranged so as to be directly above the reaction zone and the non-reaction zone of the standard reaction carrier, respectively, when the standard reaction carrier is in the ready-to-detect state.
Optionally, in one example of the above aspect, the first optical signal detector and the second optical signal detector are implemented with the same optical signal detector.
Optionally, in one example of the above aspect, the apparatus may further include: and a specific substance content determining unit for determining the content of the specific substance based on the determined light signal correction value.
Optionally, in one example of the above aspect, the apparatus may further include: and the communication unit is used for data communication between the specific substance content determining unit and the upper computer.
Optionally, in one example of the above aspect, the specific substance content determining unit is provided in a host computer or a cloud server, and the apparatus may further include: and a communication unit for data communication between the optical signal correction value determination unit and the specific substance content determination unit.
Optionally, in one example of the above aspect, the apparatus may further include: and a controller for controlling the illumination of the respective light sources, the detection of the respective light signal detectors, the data communication between the respective components in the device and/or the data communication between the components in the device and the outside.
Optionally, in one example of the above aspect, the specific substance includes one of: toxins, antibiotics, pesticides or hormones.
According to another aspect of the present application, there is provided a system for detecting the content of a specific substance in an analyte, comprising: a reaction carrier; means for detecting an optical signal value in a predetermined reaction region of a reaction carrier after reaction with an analyte as described above; and a specific substance content determining unit for determining the content of the specific substance based on the determined light signal correction value.
Optionally, in one example of the above aspect, the system may further include a host computer for presenting a specific substance content detection result to a user, and/or controlling an operation of the apparatus, an operation of the specific substance content determining unit, and an operation between the apparatus and the specific substance content determining unit.
Alternatively, in one example of the above aspect, the specific content determining unit may be implemented in the apparatus, a cloud server, or the host computer.
According to another aspect of the present application, there is provided a method for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte, comprising: detecting a first light signal detection value pair of a reaction region of the reaction carrier under a first irradiation condition and a second light signal detection value under a second irradiation condition, wherein the first irradiation condition includes respective irradiation by two groups of light sources composed of first light sources in each group of first light sources according to a specified rule, and the second irradiation condition includes irradiation by light sources related to the light sources forming the first irradiation condition; performing a predetermined process on the first optical signal detection value pair; and determining an optical signal correction value of the reaction region after the position error correction based on the predetermined processing result, the second optical signal detection value and a corresponding optical signal value correlation function, wherein the optical signal value correlation function is a functional relation between the optical signal correction value after the position error correction and the predetermined processing result and the second optical signal detection value.
Optionally, in one example of the above aspect, the method may further include: detecting a third optical signal detection value of a non-reaction region of the reaction carrier under a third irradiation condition; and based on the second optical signal detection value, the third optical signal detection value and the corresponding irradiation conditions, obtaining the second optical signal detection value after background correction, and based on the predetermined processing result, the second optical signal detection value and the corresponding optical signal value correlation function, determining an optical signal correction value of the reaction region includes: and determining the optical signal correction value of the reaction area after the position error correction based on the preset processing result, the second optical signal detection value after the background correction and a corresponding optical signal value correlation function.
Optionally, in one example of the above aspect, the method may further include: detecting a third optical signal detection value of a non-reaction region of the reaction carrier under a third irradiation condition; and determining the optical signal correction value of the reaction area after the background correction based on the optical signal correction value of the reaction area after the position error correction, the third optical signal detection value and the corresponding irradiation condition.
According to another aspect of the present application, there is provided a method for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte, comprising: detecting a first optical signal detection value of a reaction zone of the reaction carrier under a first irradiation condition; detecting a second optical signal detection value of a non-reaction area of the reaction carrier under a second irradiation condition, and acquiring an optical signal correction value of the reaction area after background correction based on the first optical signal detection value, the second optical signal detection value and the corresponding irradiation condition.
Drawings
A further understanding of the nature and advantages of the present disclosure may be realized by reference to the following drawings. In the drawings, similar components or features may have the same reference numerals.
FIG. 1 shows a block diagram of a system for detection of the content of a specific substance in an analyte according to the present application;
FIG. 2 shows a schematic diagram of a test strip according to the present application;
fig. 3 shows a block diagram of an optical signal detecting device for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a first embodiment of the present application;
fig. 4A shows a schematic diagram of one example of an arrangement of a first light source and a first optical signal detector according to a first embodiment of the application;
FIG. 4B is a schematic diagram showing the relative positional relationship of the example arrangement of FIG. 4A with respect to a region in the test strip;
FIG. 5 shows a flow chart of a method for determining an optical signal value correlation function according to a first embodiment of the application;
FIG. 6 shows a flowchart of a method for detecting an optical signal value in a reaction zone of a reaction carrier after reaction with an analyte according to a first embodiment of the present application;
fig. 7 shows a block diagram of an optical signal detecting device for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a second embodiment of the present application;
fig. 8A shows a schematic diagram of one example of an arrangement of a light source and an optical signal detector according to a second embodiment of the application;
FIG. 8B shows a schematic diagram of the relative positional relationship of the example arrangement of FIG. 8A with respect to a region in the test strip;
FIGS. 8C and 8D are schematic diagrams showing the relative positional relationship of another example arrangement of light sources and light signal detectors according to the present application with respect to regions in a test strip;
fig. 9A shows a schematic diagram of another example of an arrangement of a light source and an optical signal detector according to a second embodiment of the application;
FIG. 9B is a schematic diagram showing the relative positional relationship of the example arrangement of FIG. 9A with respect to regions in the test strip;
Fig. 10A shows a schematic diagram of another example of an arrangement of a light source and an optical signal detector according to a second embodiment of the application;
FIG. 10B is a schematic diagram showing the relative positional relationship of the example arrangement of FIG. 10A with respect to regions in the test strip;
FIG. 11 shows a flowchart of a method for detecting an optical signal value in a reaction zone of a reaction carrier after reaction with an analyte according to a second embodiment of the present application;
FIG. 12 is a flow chart illustrating a process for obtaining optical signal correction values for a reaction zone after background correction based on first and second optical signal detection values in accordance with the present application;
fig. 13 shows a block diagram of an optical signal detecting device for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a third embodiment of the present application;
fig. 14A shows a schematic diagram of one example of an arrangement of a light source and an optical signal detector according to a third embodiment of the application;
FIG. 14B is a schematic diagram showing the relative positional relationship of the example arrangement of FIG. 14A with respect to regions in the test strip;
fig. 15 is a flowchart showing one example of a method for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a third embodiment of the present application;
FIG. 16 is a flowchart showing another example of a method for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a third embodiment of the present application;
fig. 17 is a schematic view showing an external shape of a specific implementation example of an optical signal detection device for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a fourth embodiment of the present application;
FIG. 18 is an exploded view of the internal structure of the example implementation shown in FIG. 17; and
fig. 19 is an exploded view showing another specific implementation example of an internal structure of an optical signal detection device for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a fifth embodiment of the present application.
Detailed description of the preferred embodiments
The subject matter described herein will now be discussed with reference to example embodiments. It should be appreciated that these embodiments are discussed only to enable a person skilled in the art to better understand and thereby practice the subject matter described herein, and are not limiting of the scope, applicability, or examples set forth in the claims. Changes may be made in the function and arrangement of elements discussed without departing from the scope of the disclosure. Various examples may omit, replace, or add various procedures or components as desired. For example, the described methods may be performed in a different order than described, and various steps may be added, omitted, or combined. In addition, features described with respect to some examples may be combined in other examples as well.
As used herein, the term "comprising" and variations thereof mean open-ended terms, meaning "including, but not limited to. The term "based on" means "based at least in part on". The terms "one embodiment" and "an embodiment" mean "at least one embodiment. The term "another embodiment" means "at least one other embodiment". The terms "first," "second," and the like, may refer to different or the same object. Other definitions, whether explicit or implicit, may be included below. Unless the context clearly indicates otherwise, the definition of a term is consistent throughout this specification.
As used herein, the term "connected" refers to a direct mechanical, electrical, or communication connection between two components, or an indirect mechanical, electrical, or communication connection via an intermediate component. The term "electrically connected" means that two components can communicate electrically for data/information exchange. Likewise, the electrical connection may refer to a direct electrical connection between two components or an indirect electrical connection through an intermediate component. The electrical connection may be implemented in a wired manner or in a wireless manner.
Fig. 1 shows a block diagram of a system for detecting a specific substance content in an analyte (hereinafter referred to as a specific substance content detection system 1) according to the present application.
As shown in fig. 1, the specific substance content detection system 1 includes a reaction carrier 10. The reaction carrier 10 has a reaction zone and a non-reaction zone. In the reaction zone of the reaction carrier 10, a labeled reagent capable of reacting with a specific substance to be measured is provided. The labeled reagent is not disposed in the non-reaction zone of the reaction carrier 10. Since the principle of the present application is to detect the optical signal value, all the reaction carriers that generate the optical signal change after reacting with the analyte can be applied, and examples of the reaction carrier 10 may include any test paper or reagent card for detecting a large molecule or small molecule chemical substance, including, but not limited to, test paper prepared by adopting the competition method and the sandwich method principle. Examples of the test strip may include, for example, a colloidal gold test strip, a fluorescent test strip, a dry chemical test strip, and the like. Examples of reagent cards may include, for example, microfluidic reagent cards and the like. Fig. 2 shows a schematic diagram of a test strip according to the application. In the test strip shown in fig. 2, 2 reaction zones, i.e., the zones where the display strip 1 and the color-developing strip 2 are located, are shown. In general, the reaction zone may be configured to have a prescribed size. The areas of the strip other than the reaction zone are non-reaction zones, such as the blank areas of the strip in FIG. 2.
The specific substance content detection system 1 may further comprise an optical signal detection device 20. The optical signal detection device 20 is used for detecting an optical signal correction value of a reaction zone of the reaction carrier after the labeled reactant in the reaction carrier reacts with the analyte. The structure and operation of the optical signal detecting apparatus 20 will be described in detail below with reference to fig. 3 to 18.
The specific substance content detection system 1 may further include a specific substance content determination unit 30. The specific substance content determining unit 30 is electrically connected to the optical signal detecting device 20 for receiving the detected optical signal correction value of the reaction region from the optical signal detecting device 20 and determining the content of the specific substance in the object to be measured based on the detected optical signal correction value of the reaction region. For example, the specific substance content determination unit 30 may determine the specific substance content corresponding to the detected light signal correction value based on the correspondence between the light signal value and the specific substance content. The correspondence may be, for example, a correspondence table between the optical signal value and the specific substance content, or a variation curve between the optical signal value and the specific substance content, or a functional relationship between the optical signal value and the specific substance content. For example, the specific substance content determining unit 30 may determine the specific substance content by table look-up or calculation (e.g., mathematical solution). In the present application, the specific substance is a chemical substance to be detected, such as a large molecule or a small molecule compound, and for example, the specific substance may include one of the following: toxins, antibiotics, pesticides or hormones.
The specific substance content determining unit 30 and the optical signal detecting device 20 may communicate with each other by wired or wireless means. Examples of such wired communication may be, for example, optical fibers, coaxial wires, etc. Examples of the wireless communication may be, for example, bluetooth, wiFi, microwave, etc.
The specific substance content detection system 1 may further include a host computer 40. The upper computer 40 is electrically connected to the specific substance content determining unit 30, for receiving the determined specific substance content from the specific substance content determining unit 30, and presenting a specific substance content detection result to a user. Examples of the host computer 40 may include, but are not limited to, a mobile phone, a PC, a tablet, a single-chip computer, a server, or the like, for example. In addition, the upper computer 40 may also control the operation of the optical signal detecting device 20, the operation of the specific substance content determining unit 30, and the operation (e.g., interactive operation) between the optical signal detecting device and the specific substance content determining unit 30.
In the present application, the specific substance content determining unit 30 may be implemented in a software module or a hardware module. The specific substance content determining unit 30 may be implemented, for example, in a cloud server, or may be included in the optical signal detecting device 20 or in the host computer 40.
The optical signal detection means 20 may further comprise a temperature sensor for detecting temperature data when the reaction carrier is in a detection state. In the present application, the detected temperature data may be used to select a corresponding specific substance concentration versus light signal value curve, i.e. a curve for characterizing the correspondence between light signal values and specific substance contents as described above.
First embodiment
The optical signal detection device is designed according to the theoretical position of the reaction carrier when the reaction carrier is in a ready state, and in practice, various factors can cause errors between the position of the reaction area during detection and the theoretical position, such as design errors and assembly errors of test paper or reagent reaction areas during the production process, differences between the actual position of the reaction area and the theoretical position, and slight position differences of the test paper card relative to the device caused by the force intensity when the detection device inserts the reagent card. These all cause a deviation between the detected value of the optical signal and the true value of the optical signal. The scheme of the application irradiates the reaction area with light sources at different positions, uses the same photosensitive element to detect, obtains two optical signals, has correlation with the optical signal detection value and the real optical signal value, finds out the correlation by multiple tests, namely determines a functional relation, then obtains the optical signal values at different positions of the target test paper or the reagent, and the detection value is brought into the functional relation, thereby correcting position errors caused by various reasons.
Fig. 3 shows a block diagram of an optical signal detecting device 20 for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a first embodiment of the present application.
As shown in fig. 3, the optical signal detecting apparatus 20 may include at least one group of first light sources 210, each group of first light sources including at least two first light sources. At least two first light sources of each set of first light sources may be controlled separately to illuminate the same reaction zone of the reaction carrier under a first illumination condition as well as a second illumination condition, e.g. color-developing strip 1 in fig. 2. Furthermore, in the present application, each set of first light sources irradiates one different reaction zone, such as one set of first light sources (such as light sources 1404 and 1405 in fig. 14A) irradiates color developing strip 1, and another set of first light sources (such as light sources 1408 and 1409 in fig. 14A) irradiates color developing strip 2. Further, for convenience of illustration, a set of first light sources is shown as an example in fig. 3. In other examples of the application, two or more sets of first light sources may be included.
In one example of the present application, the number of first light sources included in each group of first light sources may be an even number, for example, 2, 4, 6, etc. Typically, the number of first light sources in each group of first light sources is 2. Furthermore, in one example of the present application, the positions of at least two first light sources in each group of first light sources are arranged so as to be located on both sides of the reaction region of the standard reaction carrier when the standard reaction carrier is in the detection ready state, for example, above or below both sides of the reaction region of the standard reaction carrier, i.e., projections of the arrangement positions of the at least two light sources on the reaction region of the standard reaction carrier are located on both sides of the reaction region. Preferably, the positions of the at least two first light sources are arranged so as to be symmetrically located on both sides of the reaction zone when the standard reaction carrier is in the ready-to-detect state. As shown in fig. 4A, the first light source group includes three groups of first light sources, and each group of first light sources is symmetrically arranged on both sides of one color development strip, for example, as shown in fig. 4B, first light sources 404 and 405 are symmetrically arranged on both sides of color development strip 1. In the present application, the standard reaction carrier refers to a reaction carrier that does not have a design error and an assembly error, and also does not generate an insertion error due to the standard reaction carrier not being inserted in place in the optical signal detection device when optical signal detection is performed using the standard reaction carrier.
In another example of the present application, the number of the first light sources included in each group of the first light sources may also be an odd number, for example, 3, 5, 7, etc. In this case, one of the first light sources of the group of first light sources is arranged above (e.g., directly above) the color development stripe, and the remaining even number of first light sources is symmetrically arranged on both sides of the color development stripe.
In the present application, the first irradiation condition includes two groups of light sources each consisting of each of the at least two first light sources according to a specified rule being irradiated respectively. Here, the specified rule means that two groups of light sources formed can generate light source signals of different angles for the same reaction region. For example, assuming that each group of first light sources includes 2n first light sources, two groups of light sources in the first irradiation condition are composed of k first light sources, respectively, and the positions of the two groups of light sources are symmetrical with respect to the color development stripe, where k is equal to or less than n. Further, assuming that each set of first light sources includes 2n+1 first light sources, two sets of light sources in the first irradiation condition are composed of k+1 first light sources, respectively, and also, the positions of the two sets of light sources are symmetrical with respect to the color development stripe. The second irradiation condition includes irradiation by a light source selected from the first light sources, which should have a correlation with the light source used to form the first irradiation condition, that is, a light source irradiation angle, an irradiation distance, a light source type, and the like, that is, the second irradiation condition and the first irradiation condition are correlated. For example, if the first irradiation conditions are irradiated by two light sources symmetrically arranged with respect to the reaction zone (when the standard reaction carrier is in the ready-to-detect state), the second irradiation conditions may select light sources having a similar arrangement as the light sources forming the first irradiation conditions, i.e. two other light sources symmetrically arranged with respect to the reaction zone. Preferably, the second irradiation condition may include irradiation by a light source group including at least a light source selected from the two groups of light sources. For example, the light source group in the second irradiation condition may be composed of the above 2k light sources, composed of a light source selected from the above 2k light sources, composed of the above 2k light sources and other light sources, or composed of a light source selected from the above 2k light sources and other light sources. Preferably, the second illumination condition comprises illumination by all of the at least two first light sources.
The optical signal detecting device 20 may include at least one first optical signal detector 220 for detecting a first optical signal detection value pair of the reaction region of the reaction carrier after the reaction with the analyte under the first irradiation condition and a second optical signal detection value under the second irradiation condition. Here, the first optical signal detection value pair refers to an optical signal detection value pair composed of two optical signal detection values obtained under irradiation of two sets of light sources in the first irradiation condition. In the present application, one first optical signal detector 220 may be provided for each group of first optical sources 210. In the present application, the first light source group 210 and the first optical signal detector 220 may also be collectively referred to as a first optical signal detection unit, as shown in fig. 3.
For example, assume that each set of first light sources 210 consists of two first light sources 210-1, 210-2. The first irradiation condition means that the first light sources 210-1 and 210-2 are individually turned on for irradiation, and then the corresponding optical signal detection values are detected by the first optical signal detector 220 to constitute a first optical signal detection value pair. The second irradiation condition refers to that both the first light sources 210-1 and 210-2 are turned on to irradiate, and then the corresponding optical signal detection value is detected as the second optical signal detection value by the first optical signal detector 220.
In the present application, the first light source 210 and the first light signal detector 220 may be disposed on the same plane, for example, on the same Printed Circuit Board (PCB), as shown in fig. 18. Fig. 4A shows a schematic diagram of one example of an arrangement of a first light source and a first optical signal detector according to a first embodiment of the application. Fig. 4B shows a schematic diagram of the relative positional relationship of the example arrangement of fig. 4A with respect to the region in the test strip.
Three sets of first light sources and three first light signal detectors are shown in fig. 4A, wherein the first light sources 404 and 405 constitute one set of first light sources and the first light signal detector 401 is used to detect light signal values under illumination conditions formed by the set of first light sources 404 and 405. The first light sources 406 and 407 constitute a set of first light sources, and the first light signal detector 402 is configured to detect light signal values under illumination conditions formed by the set of first light sources 406 and 407. The first light sources 408 and 409 constitute a group of first light sources, and the first light signal detector 403 is configured to detect light signal values under irradiation conditions formed by the group of first light sources 408 and 409. Furthermore, each set of light sources is used to illuminate the same reaction zone of the reaction carrier, e.g. one color-developing strip of the test strip shown in fig. 2.
The positional relationship of the first light sources 404 and 405 and the first optical signal detector 401 with respect to the color development stripes to be detected is shown in fig. 4B. As shown in fig. 4B, the first optical signal detector 401 is located above, preferably directly above, the color development strip, and the first light sources 404 and 405 are symmetrically arranged on both sides of the color development strip, specifically, on both sides of the color development strip, and projections of the arrangement positions of the first light sources 404 and 405 on the reaction region of the reaction carrier 10 are located on both sides of the color development strip.
In the present application, the first light source 210 emits light of a desired wavelength, and the light is irradiated onto the test paper and then detected by the optical signal detector 220. In one example of the present application, the first light source 210 may be a light source of a full wavelength range or a specific wavelength range, for example, an LED lamp, wherein a green LED lamp having a higher signal response value is preferable.
The first optical signal detector 220 functions to convert an optical signal in the reaction region into an electrical signal, thereby detecting the intensity of the optical signal. Examples of the first light signal detector may include a photodiode, a color sensor, a phototransistor, a monochromatic light sensor. The first optical signal detector 220 preferably uses a photodiode if the volume and cost of the optical signal detecting device 20 are taken into consideration.
In the present application, when a reaction carrier (e.g., a fluorescent test paper) needs to be detected according to fluorescence, the first light source 210 may be a fluorescence excitation light source, and the first optical signal detector 220 has a filtering characteristic matching with the wavelength of the excited fluorescence. For example, the first light source having a corresponding wavelength may be obtained by providing a filter of a specific wavelength on a light source light path of a general light source (i.e., a light source emitting white light), or a specific light source capable of emitting fluorescence may be used. In addition, preferably, in order to filter stray light, a filter may be further provided on the optical path of the fluorescent light source. Further, a filter matching the wavelength of the excited fluorescence may be added to the detection light path of the first optical signal detector 220, and a narrow band filter having a better filtering effect is preferable.
The optical signal detecting apparatus 20 may further include an optical signal correction value determining unit 230. The optical signal correction value determining unit 230 is electrically connected to the first optical signal detector 220, and is configured to receive the detected optical signal detection value from the first optical signal detector 220 and process the received optical signal detection value to obtain an optical signal correction value. Specifically, the optical signal correction value determination unit 230 performs a predetermined process on the first optical signal detection value pair received from the first optical signal detector 220; and determining the optical signal correction value of the reaction area after the position error correction based on the preset processing result, the second optical signal detection value and the corresponding optical signal value correlation function. Here, the optical signal value correlation function is used to reflect a functional relationship between the optical signal correction value after the position error correction and the predetermined processing result and the second optical signal detection value. In the present application, the predetermined process includes a difference process or a ratio process. The difference processing refers to subtraction operation of two optical signal detection values in the first optical signal detection value pair. The ratio processing refers to calculating a ratio of two optical signal detection values in the first optical signal detection value pair. In addition, the optical signal correction value determining unit 230 may be implemented in an upper computer or a server, but the data transmission is relatively slow, and the error probability is increased, so the optical signal detecting device includes the optical signal correction value determining unit in the device.
In the present application, for a fixed optical signal detector and irradiation conditions, the optical signal value correlation function may be obtained by performing a plurality of experiments to obtain corresponding optical signal detection values and optical signal reference values by adjusting the positions of the reagent cards in the optical signal detection device a plurality of times, and then performing analysis (e.g., curve fitting) or the like to find out the correlation between the optical signal reference values and the predetermined processing results and the second optical signal detection values. Furthermore, the corresponding optical signal value correlation function may also be obtained by setting different first and second illumination conditions and/or different optical signal detector types. In the present application, the optical signal reference value can be obtained by a reliable reference detection method or apparatus, for example, by a large-scale dedicated apparatus. And the optical signal detection value can be obtained by adopting various optical signal detectors available in the market.
In the present application, it is to be noted that the optical signal value correlation function is correlated with the model number of the optical signal detector, the first irradiation condition, and the second irradiation condition. As long as any one of the optical signal detector model, the first and second illumination conditions is changed, the optical signal value correlation function is also changed. Further, the irradiation condition may be determined by the number of light sources, the irradiation positions of the light sources, the kind of the light sources, and the like.
Fig. 5 shows a flow chart of a method for determining an optical signal value correlation function according to a first embodiment of the application.
As shown in fig. 5, in step S510, a first light signal detection value pair of the same reaction region (for example, display color band 1) detected with a predetermined model of light signal detector under respective illumination by the light sources in the light source pair at the set position is detected. Then, in step S520, a predetermined process is performed on the acquired pair of first optical signal detection values to acquire a predetermined process result. Next, in step S530, a second optical signal detection value detected by the predetermined model optical signal detector under irradiation of the light source pair is acquired. In step S540, the reference value of the optical signal under irradiation by the light source pair is acquired by a reliable method or apparatus.
After the predetermined processing result, the second optical signal detection value, and the corresponding optical signal reference value are obtained as above, it is determined in step S550 whether the predetermined number of trials is reached. When the predetermined number of tests is not reached, the position of the reaction carrier (reagent card) in the optical signal detection device is finely tuned in step S560, and then the process returns to step S510 to re-perform the above-described detection process for the reaction carrier subjected to the fine positional adjustment. After the predetermined number of trials is reached, in step S570, an optical signal value association function corresponding to the predetermined model of optical signal detector is determined based on the acquired predetermined processing result, second optical signal detection value, and corresponding optical signal reference value. In the above manner, an optical signal value correlation function corresponding to the predetermined model of optical signal detector under the first and second irradiation conditions can be obtained. In addition, in the present application, the corresponding optical signal value correlation function may also be obtained by adjusting the model number of the optical signal detector, the first and second irradiation conditions, and the like, respectively.
In one example of the present application, the optical signal value correlation function may be stored in the optical signal detection apparatus 20, for example, in the optical signal detection apparatus 20 in a mapping table manner, i.e., the optical signal value correlation function is mapped to the model number, the first and/or the second irradiation condition of the first optical signal detector 220 used in the optical signal detection apparatus 20. Accordingly, the optical signal detection apparatus 20 may comprise a storage unit for storing the optical signal correlation function. In another example of the application, the optical signal value association function may also be stored in an external server, for example in the form of a mapping table. The optical signal correction value determining unit 230 is electrically connected to an external server by wire or wireless to acquire a corresponding optical signal value correlation function from the external server when necessary.
In another example of the present application, the optical signal detecting apparatus 20 may further include an optical signal value correlation function generating unit for generating the optical signal value correlation function based on the history data of the predetermined processing result, the history data of the second optical signal detection value, and the history data of the corresponding optical signal detection reference value.
In one example of the present application, the optical signal correction value determining unit 230 may further include an optical signal preprocessing module (not shown) for performing a predetermined process on the first optical signal detection value pair. For example, the optical signal preprocessing module may be implemented using a subtractor or a ratio calculator. The optical signal correction value determining unit 230 may further include an optical signal correction value determining module (not shown) for determining an optical signal correction value of the reaction region after the position error correction based on the predetermined processing result, the second optical signal detection value, and the corresponding optical signal value correlation function. Here, the optical signal correction value determining unit 230 and/or its components may be implemented using a device having a computing capability, for example, using a processor, a microprocessor, a single chip microcomputer, a DSP, an FPGA, or a digital circuit unit having a computing capability.
Optionally, the optical signal detection apparatus 20 may further include a communication unit 240. Examples of the communication unit 240 may include a bluetooth device, a USB device, zigbee, an infrared unit, or a WIFI device, etc. When the specific substance content determining unit 30 is provided in the optical signal detecting device 20, the communication unit 240 is provided between the specific substance content determining unit 30 and the host computer 40 for realizing data communication between the specific substance content determining unit 30 and the host computer 40. When the specific substance content determining unit 30 is provided in the host computer 40 or the server, the communication unit 240 is provided between the optical signal correction value determining unit 230 and the specific substance content determining unit 30 for realizing data communication between the optical signal correction value determining unit 230 and the specific substance content determining unit 30.
With the above-described optical signal detection apparatus, when optical signal detection is performed for a single reaction region, by providing at least two light sources in different regions to provide light sources of different angles for the same reaction region, detecting with the same optical signal detector to obtain two kinds of optical signals, then calculating a difference or ratio operation result of the two kinds of signals, and determining an optical signal correction value by using a correlation (i.e., the above-described optical signal value correlation function) between a predetermined optical signal detection value, an optical signal correction value (i.e., the above-described optical signal reference value), and the above-described difference or ratio operation result, thereby realizing correction of a positional error of the reaction region.
Optionally, the optical signal detecting apparatus 20 may further include a timer 250 and a detection parameter setting unit 260. The timer 250 is used to count after the reaction carrier 10 enters the detection ready state. The detection parameter setting unit 260 is used for setting the detection start time, the detection cut-off time and the detection interval period of the optical signal detector according to the properties of the specific substance to be detected and the label on the reaction carrier 10. For example, the detection parameter setting unit 260 sets the detection start time, the detection cutoff time, and the detection interval period of the first optical signal detector 220.
Further, the optical signal detecting apparatus 20 may optionally further include a timing trigger unit 270 connected to the timer 250. The timing triggering unit 270 is used to trigger the timer 250 to start timing after the reaction carrier 10 enters the detection ready state. For example, when the reaction carrier 10 (e.g., a reagent strip) is put into the optical signal detecting device 20, for example, when a test paper is inserted, the timer triggering unit 270 triggers the timer 250 to start timing. The timing trigger unit 270 may be implemented in a form including, but not limited to, a mechanical trigger unit (e.g., a micro switch), a photoelectric trigger unit (e.g., a photoelectric sensor), and a magnetic trigger unit (e.g., a hall element). By using the timing triggering unit 270, and simultaneously combining the detection start time, the detection cut-off time and the detection interval period of the optical signal detector set by the detection parameter setting unit 260, the optical signal detection device 20 can be suitable for reagents with different reaction times, so as to realize detection for different specific substances.
In one example of the present application, the illumination control for the first light source 210 and the optical signal detection by the first optical signal detector 220 may be controlled manually. For example, the first light source 210 and the first light signal detector may be operated by manually turning on the power switches of the first light source 210 and the first light signal detector 220.
In another example of the present application, the optical signal detecting device 20 may further include a controller (not shown in the drawings) electrically connected to the respective light sources and the respective optical signal detectors of the optical signal detecting device 20 for controlling the irradiation of the respective light sources and the detection of the respective optical signal detectors. The controller may be electrically connected to other components of the optical signal detecting device 20 for controlling data communication between the respective components of the optical signal detecting device 20 and/or data communication between the components of the optical signal detecting device 20 and the outside. In the present application, the controller may be implemented using a processor, microprocessor, single-chip microcomputer, DSP, FPGA, or other digital circuit unit having processing capabilities.
Fig. 6 shows a flowchart of a method for detecting an optical signal value in a reaction zone of a reaction carrier after reaction with an analyte according to a first embodiment of the present application.
As shown in fig. 6, in step S610, a first optical signal detection value pair of a reaction region of a reaction carrier after reaction with an object to be measured under a first irradiation condition and a second optical signal detection value under a second irradiation condition are detected. Next, in step S620, a predetermined process is performed on the first optical signal detection value pair to acquire a predetermined process result. Then, in step S630, an optical signal correction value of the reaction region after the position error correction is determined based on the predetermined processing result and the detected second optical signal detection value, and using a corresponding optical signal value correlation function.
Optionally, in an example of the present application, before step S630, the method may further include: based on the model number of the first optical signal detector 220 used in the optical signal detecting device 20, the first and second irradiation conditions, a corresponding optical signal value association function is determined from the map. For example, in one example, the mapping table may be stored in the optical signal detection apparatus 20. In another example, the mapping table may be stored in an external server. In this case, the optical signal detecting apparatus 20 may acquire the corresponding optical signal value correlation function from the external server based on the model number of the first optical signal detector 220, the first and second irradiation conditions used in the optical signal detecting apparatus 20.
Optionally, in an example of the present application, the method may further include: at least two first light sources 210 are controlled to illuminate a predetermined reaction zone of the reaction carrier 10 according to the first and second illumination conditions.
Optionally, in an example of the present application, the method may further include: and determining the content of the specific substance based on the determined optical signal correction value.
Second embodiment
Fig. 7 shows a block diagram of an optical signal detection device 20' for detecting an optical signal correction value in a reaction region of a reaction carrier after reaction with an analyte according to a second embodiment of the present application. The embodiment shown in fig. 7 is an improvement to the optical signal detection device 20 shown in fig. 3.
Compared to fig. 3, the optical signal detecting device 20' shown in fig. 7 is different in that each of at least one set of first light sources is controlled to irradiate the reaction region of the reaction carrier only under the first irradiation condition, and the first irradiation condition in the present embodiment is different from the first irradiation condition in the first embodiment. In addition, at least one set of second light sources 210 'and at least one second light signal detector 220' are added in fig. 7. Further, the optical signal correction value determining unit 230' shown in fig. 7 is different from the optical signal correction value determining unit 230 in fig. 3. The other units in fig. 7 are identical to the corresponding units shown in fig. 3. For simplicity of description, only the differences are described below, and the same units will not be described again.
As shown in fig. 7, the optical signal detection means 20' comprises at least one set of first light sources 210, each set of first light sources being adapted to illuminate the same reaction zone of the reaction carrier 10 under a first illumination condition. Here, each of the at least one set of first light sources 210 may include one or more first light sources. In the case where each set of first light sources 210 includes a plurality of first light sources for illuminating the same color-developing stripe (i.e., the same reaction region). In the present application, each of the first light source groups 210 corresponds to one color developing stripe (i.e., one reaction region) for irradiating the corresponding reaction region of the reaction carrier 10, for example, color developing stripes 1, 2, etc., under the first irradiation condition. In the present embodiment, the first irradiation condition refers to an irradiation condition in which a single light source group consisting of the first light sources selected from the first light source group 210 is individually irradiated. The at least one first optical signal detector 220 is configured to detect a first optical signal detection value of the reaction area under a first irradiation condition after the reaction with the object to be detected.
The optical signal detection apparatus 20' may further comprise at least one set of second light sources 210' and at least one second optical signal detector 220'. Each of the at least one set of second light sources 210' is adapted to illuminate the same non-reaction zone of the reaction carrier 10 under a second illumination condition. Here, each set of second light sources 210' may include one or more second light sources for illuminating the same non-reaction area (i.e., the same test paper blank area). The at least one set of second light sources 210 'may also include a plurality of second light source sets, each second light source set 210' corresponding to one non-reaction region for illuminating a corresponding non-reaction region of the reaction carrier 10, e.g., a test paper blank region, under the second illumination condition. Accordingly, at least one second optical signal detector 220' is configured to detect a second optical signal detection value of a corresponding non-reaction region of the reaction carrier 10 under a second irradiation condition. Here, one second optical signal detector 220 'may be provided for each group of second optical sources 210'. Unlike the second irradiation conditions in the first embodiment, the second irradiation conditions in the present embodiment refer to irradiation conditions of a single light source group composed of the second light sources in each group of the second light sources 210' according to a predetermined rule.
Then, the optical signal correction value determining unit 230' determines the optical signal correction value of the reaction region after the background correction based on the first optical signal detection value and the second optical signal detection value and the corresponding irradiation conditions (i.e., the first irradiation condition and the second irradiation condition in the present embodiment).
In one example of the application, the first light source set 210 and the second light source set 210 'may be implemented with different light sources and the at least one first light signal detector 220 and the at least one second light signal detector 220' may be implemented with different light signal detectors for the same reaction zone, e.g. color stripe 1. Fig. 8A shows a schematic diagram of one example of an arrangement of a light source and an optical signal detector according to a second embodiment of the present application. FIG. 8B shows a schematic diagram of the relative positional relationship of the example arrangement of FIG. 8A with respect to regions in the test strip. As shown in fig. 8A and 8B, the positions of the first light source 804 and the second light source 806 are arranged so as to be located directly above the reaction region (color development strip 1) and the non-reaction region (test paper blank region) of the standard reaction carrier when the standard reaction carrier is in the detection ready state, and the positions of the first light signal detector 801 and the second light signal detector 802 are arranged so as to be located directly above the reaction region (color development strip 1) and the non-reaction region (test paper blank region) when the reaction carrier 10 is in the detection ready state, respectively. The first optical signal detector 801 and the second optical signal detector 802 may be arranged at other suitable positions in the present application. In fig. 8B, the second light source 806 and the corresponding second light signal detector 802 are shown disposed directly above the non-reaction area (test paper blank area) between the color developing strip 1 and the color developing strip 2. In other examples of the application, the second light source 806 and the corresponding second light signal detector 802 may be disposed directly above the non-reaction area (test paper blank area) outside of the color development strips 1 and 2, for example, directly above the left side area of all color development strips (color development strips 1 and 2) as in fig. 8C, or directly above the right side area of all color development strips (display strips 1 and 2) as in fig. 8D.
In one example of the application, the first light source set 210 and the second light source set 210 'may be implemented with the same light source and the at least one first light signal detector 220 and the corresponding at least one second light signal detector 220' may be implemented with different light signal detectors for the same reaction zone, e.g. color stripe 1. The same light source is positioned to be located in an area between the first light signal detector 220 and the second light signal detector 220'. Fig. 9A shows a schematic diagram of another example of an arrangement of a light source and an optical signal detector according to a second embodiment of the application. Fig. 9B shows a schematic diagram of the relative positional relationship of the example arrangement of fig. 9A with respect to the region in the test strip.
In one example of the application, the first light source set 210 and the second light source set 210 'may be implemented with different light sources and the at least one first light signal detector 220 and the corresponding at least one second light signal detector 220' may be implemented with the same light signal detector for the same reaction zone, e.g. color stripe 1. The same optical signal detector is positioned so as to be located above the reaction zone (color development strip 1) of the standard reaction carrier when the standard reaction carrier is in the ready state for detection, or at any position above the standard reaction carrier. Fig. 10A shows a schematic diagram of another example of an arrangement of a light source and an optical signal detector according to a second embodiment of the present application. FIG. 10B shows a schematic diagram of the relative positional relationship of the example arrangement of FIG. 10A with respect to regions in the test strip.
Fig. 11 shows a flowchart of a method for detecting an optical signal value in a reaction region of a reaction carrier after reaction with an analyte according to a second embodiment of the present application.
As shown in fig. 11, in step S1110, a first optical signal detection value of the reaction region of the reaction carrier 10 after the reaction with the analyte under the first irradiation condition is detected. Wherein the first illumination conditions are provided by the corresponding first light sources. Next, in step S1120, a second optical signal detection value of the non-reaction area of the reaction carrier 10 under a second irradiation condition is detected. Wherein the second illumination condition is provided by a corresponding second light source. Then, in step S1130, an optical signal correction value of the reaction region after the background correction is determined based on the first optical signal detection value and the second optical signal detection value and the corresponding irradiation conditions (i.e., the first irradiation condition and the second irradiation condition mentioned in the second embodiment).
FIG. 12 is a flow chart illustrating a process for determining an optical signal correction value for a reaction zone after background correction based on first and second optical signal detection values in accordance with the present application.
As shown in fig. 12, in step S1131, it is determined whether the first irradiation condition and the second irradiation condition are the same. If the first irradiation condition and the second irradiation condition are different, the second optical signal detection value is converted into an optical signal detection value under the same irradiation condition as the first irradiation condition at step S1133. For example, assuming that the first irradiation condition is irradiation with 2 light sources of the same model, and the second irradiation condition is irradiation with 1 light source of the same model in the first irradiation condition, the second light signal detection value is multiplied by 2 as the converted light signal detection value. Further, more preferably, when the irradiation conditions are considered to be the same, factors that influence the irradiation conditions such as illuminance of the light source, angle of the light source, and the like may also be considered. Then, in step S1135, the converted second optical signal detection value is subtracted from the first optical signal detection value as an optical signal correction value of the reaction region after the background correction.
When the determination result in S1131 is that the first irradiation condition and the second irradiation condition are the same, in step S1135, the second optical signal detection value is subtracted from the first optical signal detection value as the optical signal correction value of the reaction region after the background correction.
Optionally, in another example of the present application, the method for detecting an optical signal value in a reaction area of a reaction carrier after a reaction with an analyte may further include: controlling at least one set of first light sources 210 to illuminate the reaction zone of the reaction carrier 10 according to the first illumination conditions; and controlling at least one set of second light sources 210' to illuminate the non-reaction region of the reaction carrier 10 according to the second illumination condition.
Optionally, in another example of the present application, the method may further include: and determining the content of the specific substance based on the determined optical signal correction value.
With the optical signal detection apparatus and method shown in fig. 7 to 12, by adding the second light source and/or the second optical signal detection apparatus for detecting the optical signal value of the non-reaction region (i.e., the background region of the reaction carrier), and subtracting the second optical signal detection value from the first optical signal detection value of the detected reaction region, the influence of the optical signal generated by the background region of the reaction carrier (reagent strip or test strip) and/or the unreacted reactant and/or sample after the reaction, i.e., the influence of the background optical signal, can be eliminated, thereby making the detected optical signal correction value of the reaction region more accurate.
Third embodiment
Fig. 13 shows a block diagram of an optical signal detection device 20″ for detecting an optical signal correction value in a reaction region of a reaction carrier after reaction with an analyte according to a third embodiment of the present application. The embodiment shown in fig. 13 is an improvement to the optical signal detection device 20 shown in fig. 3.
The optical signal detection arrangement 20 "shown in fig. 13 differs from that of fig. 3 in that at least one set of second light sources 210 'and at least one second optical signal detector 220' are added. Further, the optical signal correction value determining unit 230″ shown in fig. 13 is different from the optical signal correction value determining unit 230 in fig. 3. The other units in fig. 13 are identical to the corresponding units shown in fig. 3. For simplicity of description, only the differences are described below, and the same units will not be described again.
The first light source group 210 in fig. 13 is identical to the first light source group in fig. 3, and will not be described here. At least one first optical signal detector 220 detects a first optical signal detection value pair of a corresponding reaction region under a first irradiation condition and a second optical signal detection value under a second irradiation condition after reacting with the object to be measured. Here, the first irradiation condition and the second irradiation condition in the present embodiment are similar to those described in the first embodiment. The structure and function of at least one set of second light sources 210' in fig. 13 is identical to the at least one set of second light sources in fig. 7. At least one second optical signal detector 220' is configured to detect a third optical signal detection value of the non-reaction area of the reaction carrier 10 under a third irradiation condition. Here, the third irradiation condition in the present embodiment is similar to the second irradiation condition described in the second embodiment, and may be provided by the first light source group or by a separate second light source group.
In one example of the present application, background signal subtraction may be performed on the optical signal values of the reaction region, and then correction may be performed with respect to the position error of the reaction region. Specifically, the optical signal correction value determination unit 230″ is configured to perform predetermined processing on the first optical signal detection value pair to obtain a predetermined processing result. Next, the optical signal correction value determining unit 230″ obtains the second optical signal detection value after the background correction based on the second optical signal detection value and the third optical signal detection value. In one example of the application, the above described correction function may be implemented using a background correction module. Then, the optical signal correction value determining unit 230″ determines the optical signal correction value of the reaction region after the position error correction based on the predetermined processing result, the second optical signal detection value after the background correction, and the corresponding optical signal value correlation function.
In another example of the present application, the optical signal value of the reaction region may be corrected for the position error of the reaction region, and then subtracted from the background signal. Specifically, the optical signal correction value determining unit 230″ may perform a predetermined process on the first optical signal detection value pair of the reaction region to obtain a predetermined process result, and determine the second optical signal detection value of the reaction region after the position error correction based on the predetermined process result, the second optical signal detection value, and the corresponding optical signal value correlation function. Furthermore, the optical signal correction value determining unit 230″ is further configured to obtain the optical signal correction value of the reaction region after the background correction based on the determined optical signal correction value of the reaction region after the position error correction, the third optical signal detection value, and the corresponding irradiation conditions (i.e., the second irradiation condition and the third irradiation condition).
In the present application, the optical signal correction value determining unit 230″ may be implemented by a processor, a microprocessor, a single chip microcomputer, a DSP, an FPGA, or other digital circuit unit having processing capability.
Fig. 14A shows a schematic diagram of one example of an arrangement of a light source and an optical signal detector according to a third embodiment of the present application. Fig. 14B shows a schematic diagram of the relative positional relationship of the example arrangement of fig. 14A with respect to the region in the test strip. In fig. 14A, 2 display strips and one test paper blank area are shown, together with a corresponding 2 sets of first light sources (1404,1405,1408 and 1409) and a set of second light sources (1406 and 1407).
Fig. 15 is a flowchart showing one example of a method for detecting an optical signal correction value in a reaction region of a reaction carrier after reaction with an analyte according to a third embodiment of the present application.
As shown in fig. 15, in step S1510, a first optical signal detection value pair of a corresponding reaction region of a reaction carrier under a first irradiation condition and a second optical signal detection value under a second irradiation condition are detected by at least one first optical signal detector. In step S1520, a predetermined process is performed on the first optical signal detection value pair of the reaction region to obtain a predetermined process result, and then, in step S1530, an optical signal correction value of the reaction region after the position error correction is determined based on the predetermined process result, the second optical signal detection value, and the corresponding optical signal value correlation function for the reaction region.
Next, in step S1540, a third optical signal detection value of the corresponding non-reaction region of the reaction carrier under a third irradiation condition is detected by at least one second optical signal detector.
Then, in step S1550, the optical signal correction value of the reaction region after the background correction is determined based on the determined optical signal correction value of the reaction region after the position error correction, the third optical signal detection value, and the corresponding irradiation conditions (i.e., the second irradiation condition and the third irradiation condition). For example, assuming that the second irradiation condition and the third irradiation condition are the same, the optical signal correction value of the non-reaction region is subtracted from the optical signal correction value of the reaction region as the optical signal correction value of the reaction region after the background correction. If the second irradiation condition and the third irradiation condition are different, converting the optical signal correction value of the non-reaction region into a converted optical signal correction value of the non-reaction region under the same irradiation as the second irradiation condition, and then subtracting the converted optical signal correction value of the non-reaction region from the optical signal correction value of the reaction region as the optical signal correction value of the reaction region after the background correction.
Fig. 16 is a flowchart showing another example of a method for detecting an optical signal correction value in a reaction region of a reaction carrier after reaction with an analyte according to the third embodiment of the present application.
As shown in fig. 16, in step S1610, a first optical signal detection value pair of a corresponding reaction region of a reaction carrier under a first irradiation condition and a second optical signal detection value under a second irradiation condition are detected by at least one first optical signal detector. In step S1620, a predetermined process is performed on the first optical signal detection value pair of the reaction region to obtain a predetermined process result. In step S1630, a third optical signal detection value of the non-reaction region of the reaction carrier under a third irradiation condition is detected by at least one second optical signal detector. In step S1640, the second optical signal detection value of the reaction region after the background correction is determined based on the second optical signal detection value of the reaction region, the third optical signal detection value of the non-reaction region, and the corresponding irradiation conditions (i.e., the second irradiation condition and the third irradiation condition). The determination of the second optical signal detection value after the background correction can be referred to the determination described above with reference to fig. 12. Then, in step S1650, the optical signal correction value of the reaction region after the positional error correction is determined based on the predetermined processing result for the reaction region, the second optical signal detection value after the background correction, and the corresponding optical signal value correlation function.
Further, similar to the methods shown in fig. 6 and 11, the methods shown in fig. 15 and 16 may also include corresponding control processes and specific substance content determination processes. And will not be described in detail herein.
The system for detecting the content of a specific substance in an analyte, the apparatus for detecting the correction value of an optical signal in a reaction zone of a reaction carrier after reaction with the analyte, and the method according to the present application are described above with reference to fig. 1 to 16.
The apparatus for detecting an optical signal correction value in a reaction region of a reaction carrier after reaction with an analyte according to the present application will be described further below using specific implementation examples.
Fourth embodiment
Fig. 17 is a schematic external shape showing a specific implementation example of an optical signal detection device 1700 for detecting an optical signal correction value in a predetermined reaction region of a reaction carrier after reaction with an analyte according to the fourth embodiment of the present application. As shown in fig. 17, the optical signal detecting apparatus 1700 includes an indicator lamp 1701, a USB charging port 1702, a power key 1703, a body 1704, and a cap 1705.
Fig. 18 shows an exploded view of the internal structure of the concrete implementation example shown in fig. 17. As shown in fig. 18, the optical signal detection apparatus 1700 may include an optical signal detection unit 1712 composed of a light source and an optical signal detector. Here, the light source and the light signal detector in the light signal detection unit 1712 may take various combinations as described above with reference to fig. 3, 7, and 13.
The optical signal detecting apparatus 1700 may further include a bluetooth module 1711 for data communication of the optical signal detecting apparatus 1700 with the outside. Here, the bluetooth module 1711 may be replaced with various other types of communication units.
The optical signal detection apparatus 1700 may further comprise a processor 1713 for implementing processing of the respective optical signal detection values detected by the optical signal detection unit 1712. For example, the processor 1713 may implement the functions of the optical signal correction value determination unit described above. The processor 1713 may also perform the functions of the specific substance content determination unit and/or the controller described above.
The optical signal detection apparatus 1700 may also include a micro switch 1714. Here, the micro switch 1714 may implement the functions of the timer timing triggering unit described above. The optical signal detection apparatus 1700 may further include a USB charging port 1715 and a power switch 1716.
Further, in the specific implementation example shown in fig. 18, the bluetooth module 1711, the optical signal detection unit 1712, the processor 1713, the micro switch 1714, the USB charging port 1715, and the power switch 1716 are disposed on the same printed circuit board 1710. In other examples of the application, the modules or units may not be disposed on the same printed circuit board,
In addition, the optical signal detecting device 1700 may be further provided with a structural support 1709 for fixing the printed circuit board 1710 to the structural support 1709 through positioning holes and snaps. In addition, the structural support 1709 may also be provided with a reagent card receiving space for receiving a reagent card 1708. In addition, the structural support 1709 may be optionally provided with a battery receiving space for receiving a lithium battery 1707. For example, the lithium battery 1707 is secured within a corresponding recess of the structural support 1709 by an adhesive process. When charging is required, the lithium battery 1707 is charged through the USB charging port 1705.
In addition, the optical signal detecting apparatus 1700 may further include a light shielding member on the structural support 1709 for eliminating the influence of stray light interference and interference between the detecting units on the detection result. For example, a light shield is provided between the reagent card 1708 and the PCB 1710. Specifically, for example, a light shielding member is arranged at a position corresponding to the light source and the optical signal detector on the PCB 1710.
In performing the detection, the reagent card 1708 can be inserted into the reagent card accommodation space of 1709 and accurately positioned. Upon detecting that the reagent card is inserted in place (i.e., the reaction carrier is in a ready-to-detect state), the microswitch 1704 is triggered to start timing using a timing function provided in the processor 1703, thereby completing the detection of the optical signal values at the respective periods of the reaction.
The processor 1703 may obtain relevant measurement data in response to the instruction and report the obtained measurement data to the host computer, or may control each component in the optical signal detection apparatus 1700 to perform a corresponding function and aggregate the obtained data.
The device can be used for detecting the test paper with color change after reacting with the object to be detected, thereby detecting the content of the object to be detected.
Fifth embodiment
Fig. 19 is an exploded view showing another specific implementation example of an internal structure of an optical signal detection device for detecting an optical signal correction value in a predetermined reaction region of a reaction carrier after reaction with an analyte according to a fifth embodiment of the present application. Fig. 19 is a modification of the specific implementation example shown in fig. 18, except that the light source is changed to a fluorescence excitation light source, and the detection element is changed to a detection element capable of detecting excited fluorescence, for example, a filter for a specific wavelength is provided on the light source light path of a normal light source (i.e., a light source that emits white light), so that a first light source (e.g., a fluorescence excitation light source) having a corresponding wavelength can be implemented, and a filter is provided on the light path of the light signal detector 220, for light signal value detection (e.g., light signal detection for a fluorescent test paper) of a corresponding wavelength. As shown in fig. 19, a filter matched with the excited fluorescent light source may be added at a corresponding position of the optical signal detector 220 of the light shielding member 1911, and the filter is used for wavelength filtering of the light emitted from the light source. And a filter can be added below the light source to filter stray light. With the optical signal detection device shown in fig. 19, it is possible to apply to optical signal detection of a fluorescent test paper or a fluorescent reagent card.
Sixth embodiment
And (3) detecting instrument performance:
the basis for making a quantitative determination is the precision of the system measurement, typically characterized by the CV values of multiple measurements.
The CV values of parallel wells were verified by measuring colloidal gold quality control cards of different concentrations using the present device and a reference measurement was performed on the same quality control card using the bench Jin Biaoyi (Hua Kerui HR201 Jin Biaoyi) measurement. The specific experimental protocol and data are as follows:
and testing by using prepared quality control cards with concentration gradients of 5mIU/ml,25mIU/ml and 45mIU/ml of two batches, wherein 10 quality control cards are used for each batch of concentration points, 20 quality control cards are used for each concentration point, and CV value statistics is carried out by respectively measuring and obtaining each measurement value. A typical set of experimental data statistics is listed as follows:
the data can find that the scheme detection result without background subtraction and position correction has poor precision, only qualitative and semi-quantitative judgment can be carried out, the measured CV value is greatly improved after the background subtraction of the system, and the measured CV value is further improved after the position correction; the CV value of the data after the position correction and the background subtraction correction is further optimized. Therefore, it can be concluded that the quantitative determination can be performed by the method, the background subtraction method can overcome batch background difference of NC films, and the position correction method can overcome the position error of the quality control card strip, so that the CV value of the detection result is obviously improved; through the simultaneous application of the two methods, the CV value is smaller, namely the detection result is more stable, and the level of the desk-top gold mark analyzer is reached or is close to that of the desk-top gold mark analyzer.
The optical signal detection device according to the present application can be applied to detection in various fields such as food safety field, medical field, environmental field, etc., in which the optical signal detection device according to the present application can be used for detecting the content of toxins, antibiotics, pesticides or hormones, etc., for example.
In particular, the above-mentioned optical signal detection device may be used for detecting the content of toxins, antibiotics, pesticides or hormones in human or animal body fluids (urine, blood, milk, saliva, tears) or foods (meat, milk, eggs, grains, tea, fruits and vegetables) or feeds.
The optical signal detecting apparatus according to the present application may be manufactured as a portable micro device, for example, a device having a length, width, height of 13×3×2.2cm or less and a weight of 220g or less.
In addition, due to portability and rapidity of the optical signal detection device, the optical signal detection device described above may be used for home detection, such as home quantitative or qualitative detection, and may be used for rapid detection, such as rapid quantitative or qualitative detection.
The detailed description set forth above in connection with the appended drawings describes exemplary embodiments, but does not represent all embodiments that may be implemented or fall within the scope of the claims. The term "exemplary" used throughout this specification means "serving as an example, instance, or illustration," and does not mean "preferred" or "advantageous over other embodiments. The detailed description includes specific details for the purpose of providing an understanding of the described technology. However, the techniques may be practiced without these specific details. In some instances, well-known structures and devices are shown in block diagram form in order to avoid obscuring the concepts of the described embodiments.
The previous description of the disclosure is provided to enable any person skilled in the art to make or use the disclosure. Various modifications to the disclosure will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other variations without departing from the scope of the disclosure. Thus, the disclosure is not limited to the examples and designs described herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (22)
1. An apparatus for detecting an optical signal value in a reaction zone of a reaction carrier after reaction with an analyte, comprising:
at least one set of first light sources, each set of first light sources comprising at least two first light sources, the at least two first light sources being controllable to illuminate the same reaction zone of the reaction carrier under a first illumination condition and a second illumination condition, the first illumination condition being different from the second illumination condition, the reaction carrier being composed of a reaction zone and a non-reaction zone, the non-reaction zone being a blank zone or a background zone;
at least one first optical signal detector for detecting a first optical signal detection value pair of the reaction region under the first irradiation condition and a second optical signal detection value under the second irradiation condition; and
An optical signal correction value determining unit configured to perform a predetermined process on the first optical signal detection value pair; and determining an optical signal correction value of the reaction region after the position error correction based on a predetermined processing result, the second optical signal detection value and a corresponding optical signal value correlation function,
wherein the optical signal value correlation function is used for reflecting the functional relation between the optical signal correction value subjected to the position error correction and the preset processing result and the second optical signal detection value, is generated based on the history data of the preset processing result, the history data of the second optical signal detection value and the history data of the corresponding optical signal detection reference value, is correlated with the model of the first optical signal detector and the first and second irradiation conditions, and
wherein the first irradiation conditions include respective irradiation by two groups of light sources composed of first light sources in the each group of first light sources in accordance with a specified rule, and the second irradiation conditions include irradiation by light sources related to the light sources forming the first irradiation conditions;
the specified rule means that two groups of light sources formed can generate light source signals with different angles aiming at the same reaction area;
The second irradiation condition includes irradiation by a light source group including at least a light source selected from the two groups of light sources;
the second illumination condition includes illumination by all of the at least two first light sources;
the positions of the at least two first light sources are arranged so as to be located on both sides of the reaction zone of the standard reaction carrier when the standard reaction carrier is in the ready-to-detect state.
2. The apparatus of claim 1, wherein the predetermined process comprises a difference process or a ratio process.
3. The apparatus of claim 1, further comprising:
a detection parameter setting unit, configured to set a detection start time, a detection cut-off time, and a detection interval period of the first optical signal detector according to properties of a specific substance to be detected and a marker on the reaction carrier;
a timer; and
and the timing triggering unit is connected with the timer and is used for triggering the timer to start timing after the reaction carrier enters the detection ready state.
4. The apparatus of claim 1, wherein the first light source is a light source having a full wavelength range or a specific wavelength range.
5. The apparatus of claim 1, wherein the first light source is a fluorescence excitation light source and the first light signal detector has a filtering characteristic that matches the excited fluorescence wavelength.
6. The device of claim 1, wherein the positions of the at least two first light sources are arranged so as to be symmetrically located on both sides of the reaction zone of a standard reaction carrier when the standard reaction carrier is in a ready-to-detect state.
7. The apparatus of claim 1, further comprising:
and the temperature sensor is used for detecting temperature data when the reaction carrier is in a detection state.
8. The apparatus of claim 1, further comprising:
at least one second optical signal detector for detecting a third optical signal detection value under a third irradiation condition with a non-reaction region of the reaction carrier, and
the optical signal correction value determination unit further includes: a background correction module for obtaining the second optical signal detection value after background correction based on the second optical signal detection value, the third optical signal detection value and the corresponding irradiation conditions, and
the optical signal correction value determination unit is used for: determining an optical signal correction value of the reaction area after position error correction based on the preset processing result, the corrected second optical signal detection value and a corresponding optical signal value correlation function; or alternatively
The optical signal correction value determination unit is further configured to: and determining the optical signal correction value of the reaction area after the background correction based on the determined optical signal correction value of the reaction area after the position error correction, the third optical signal detection value and the corresponding irradiation condition.
9. The apparatus of claim 8, wherein the second irradiation condition and the third irradiation condition are the same.
10. The apparatus of claim 9, wherein the second illumination condition and the third illumination condition are provided by the same light source or by different light sources.
11. The apparatus of claim 8, wherein the first optical signal detector and the second optical signal detector are implemented using the same optical signal detector.
12. The apparatus of claim 8, further comprising:
and a specific substance content determining unit for determining the content of the specific substance based on the determined light signal correction value.
13. The apparatus of claim 12, further comprising:
and the communication unit is used for data communication between the specific substance content determining unit and the upper computer.
14. The apparatus of claim 12, wherein the specific substance content determining unit is provided in a host computer or a cloud server, and the apparatus further comprises:
And a communication unit for data communication between the optical signal correction value determination unit and the specific substance content determination unit.
15. The apparatus of any of claims 1-14, further comprising:
and a controller for controlling the illumination of the respective light sources, the detection of the respective light signal detectors, the data communication between the respective components in the device and/or the data communication between the components in the device and the outside.
16. The device of any one of claims 1-14, wherein the particular substance comprises one of:
toxins, antibiotics, pesticides or hormones.
17. A system for detection of the content of a specific substance in an analyte, comprising:
a reaction carrier; the device of any one of claims 1-16; and
and a specific substance content determining unit for determining the content of the specific substance based on the determined light signal correction value.
18. The system of claim 17, further comprising:
and the upper computer is used for presenting a specific substance content detection result to a user and/or controlling the operation of the device, the operation of the specific substance content determining unit and the operation between the device and the specific substance content determining unit.
19. The system of claim 18, wherein the specific substance content determination unit is implemented in the device, a cloud server, or the host computer.
20. A method for detecting an optical signal value in a reaction zone of a reaction carrier after reaction with an analyte using the apparatus of claim 1, comprising:
detecting a first light signal detection value pair of a reaction region of the reaction carrier under a first irradiation condition and a second light signal detection value under a second irradiation condition, wherein the first irradiation condition includes respective irradiation by two groups of light sources composed of first light sources in each group of first light sources according to a specified rule, and the second irradiation condition includes irradiation by light sources related to the light sources forming the first irradiation condition;
performing a predetermined process on the first optical signal detection value pair; and
determining an optical signal correction value of the reaction area after position error correction based on the predetermined processing result, the second optical signal detection value and a corresponding optical signal value correlation function,
wherein the optical signal value correlation function is a function relation between the optical signal correction value subjected to the position error correction and the predetermined processing result and the second optical signal detection value.
21. The method of claim 20, further comprising:
detecting a third optical signal detection value of a non-reaction region of the reaction carrier under a third irradiation condition;
acquiring the second optical signal detection value after background correction based on the second optical signal detection value, the third optical signal detection value and corresponding irradiation conditions, and
determining an optical signal correction value of the reaction region after the position error correction based on the predetermined processing result, the second optical signal detection value, and a corresponding optical signal value correlation function includes:
and determining an optical signal correction value of the reaction area after position error correction based on the preset processing result, the corrected second optical signal detection value and a corresponding optical signal value correlation function.
22. The method of claim 20, further comprising:
detecting a third optical signal detection value of a non-reaction region of the reaction carrier under a third irradiation condition; and
and determining the optical signal correction value of the reaction area after the background correction based on the optical signal correction value of the reaction area after the position error correction, the third optical signal detection value and the corresponding irradiation condition.
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