CN110558333A - Composition for killing prodenia litura by metarhizium anisopliae and metaflumizone - Google Patents
Composition for killing prodenia litura by metarhizium anisopliae and metaflumizone Download PDFInfo
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- CN110558333A CN110558333A CN201910903905.2A CN201910903905A CN110558333A CN 110558333 A CN110558333 A CN 110558333A CN 201910903905 A CN201910903905 A CN 201910903905A CN 110558333 A CN110558333 A CN 110558333A
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
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Abstract
The invention belongs to the technical field of biocides containing microbial fungi, and particularly relates to a composition for killing prodenia litura. The composition comprises metarhizium anisopliae and metaflumizone. The active ingredients metarhizium anisopliae and metaflumizone in the composition have obvious synergistic effect on prodenia litura.
Description
Technical Field
the invention belongs to the technical field of biocides containing microbial fungi, and particularly relates to a composition for killing prodenia litura by metarhizium anisopliae and metaflumizone.
background
Spodoptera litura (Fabricius), also known as Neurospora litura, Spodoptera litura, Trichoplusia, Nelumbo nucifera, and the like, belong to the class Insecta, Lepidoptera, and Spodoptera litura (the temporal and spatial distribution of Spodoptera litura) in China is summarized, Wuhua-chang et al, Anhui agricultural science, volume 44, No. 9, column 2, lines 1-3 on the left column of page 142, and open day 2016, month 06, 27. The spodoptera litura larvae are 6-year old and are pseudodead. Entering a binge-eating period after 4 years, eating large-area host plant leaves when rampant and migrating to other places. Adult length 14-20mm, wing exhibition 35-46mm, body dark brown, there are white clumps on the chest back, preceding wing grey brown, the decorative pattern is many, interior horizontal line and outer horizontal line white, be wavy, there is obvious white oblique broad band line in the middle, so called prodenia litura ("peach garden prodenia litura emergence condition and prevention and cure measure", Li Jing et al, Sichuan agricultural science, 9 th 2010, page 53 left column, 1 st paragraph 1-9 line, 24 th 11.2010-24 th).
The prodenia litura is a world-wide binge eating pest which intermittently occurs, mainly takes larvae as a whole plant, is common in all parts of China, particularly has great harm and serious harm to Yangtze river basin and Huaihe river basin, has a wide host range, can harm 389 plants of 109 families, host plants comprise areca taro, strawberry, Chuzhou chrysanthemum, ligusticum wallichii, peanut, Chinese cabbage, welsh onion, soybean, tomato, sweet potato, citrus, florists chrysanthemum, medlar, lotus, black-skinned white gourd, cauliflower, Chinese yam, okra, pitaya, ginger, carnation, water spinach, pepper, plum, pear, lotus root, asparagus lettuce, cotton, cassava, alfalfa, grape, navel orange, mulberry tree, Chinese rose, tobacco, peach, watermelon, broccoli, rubber, sunflower, flax, ginkgo, camellia oleifera and the like, wherein the victimbery of cruciferous vegetables, green plants and the like are the heaviest (the countermeasure for preventing and controlling the drug resistance of the prodenia litura and the development of the prodenia litura, zhou Xiao Mei et al, insect knowledge, vol 39, No. 2, page 98, left column, lines 1-3, published 2002, month 01, 08; "research on bioactivity and field control effect of azadirachtin emulsifiable solution on prodenia litura", Dynasty and the like, apply the ecology newspaper, volume 16, 6 th in 2005, 1 st to 3 th lines in the left column of 1095, 7 months and 20 days in 2005; "artificial breeding technique of prodenia litura", yellow brilliant monarch, etc., in the silkworm industry, 32 nd volume, 3 rd phase, 2011, 1 st segment, line 1-7, left column, page 76, published 2011, 12 months and 31 days; "the summary of the spatiotemporal distribution of Spodoptera litura (Spodopteralitura) in China", Wuhuan constant et al, Anhui agricultural science, volume 44, No. 9, page 143, left column, No. 3, lines 1-6, published 2016, No. 06, No. 27).
The cluster leaf back of the prodenia litura larvae gnaw at the young age, the cluster leaf back of the prodenia litura larvae is scattered to be harmful to leaves and tender stems after the 3 rd age, the old larvae can eat fruits, the feeding property of the prodenia litura larvae is both miscellaneous and harmful to various organs, and the prodigy forms overeating at the old age (peach orchard prodenia litura occurrence condition and control measures, Li Jing, etc., Sichuan agricultural science, 9 th 2010, 1 st section of the left column at page 53, 11-14 lines, and 24 days of 2010-24 days).
Therefore, the research on the medicament capable of effectively preventing and treating prodenia litura is of great significance.
Disclosure of Invention
In view of the above, the present invention aims to provide a prodenia litura killing composition, wherein the active ingredients metarhizium anisopliae and metaflumizone in the composition have a significant synergistic effect on prodenia litura.
in order to achieve the purpose, the technical scheme of the invention is as follows:
The active ingredients of the composition for killing prodenia litura comprise metarhizium anisopliae and metaflumizone.
The prodenia litura killing refers to the ability of a substance to increase the mortality rate or inhibit the growth rate of insects of the insecta, lepidoptera, noctuidae, prodenia and prodenia species.
In the research process, the inventor unexpectedly finds that the active ingredients of the metarhizium anisopliae and the metaflumizone in the composition comprising the metarhizium anisopliae and the metaflumizone have a remarkable synergistic effect on the prodenia litura.
Further, the metarhizium anisopliae is metarhizium anisopliae CQMa 421.
Further, the proportion of the metarhizium anisopliae and the metaflumizone is 1 multiplied by 1000 hundred million spores to 14 multiplied by 1000 million spores: 8-32g of metaflumizone.
Furthermore, the proportion of metarhizium anisopliae and metaflumizone is 4 x 1000 hundred million-12 x 1000 hundred million spores: 12-28g metaflumizone.
Further, the proportion of metarhizium anisopliae and metaflumizone is 6 x 1000 hundred million-10 x 1000 hundred million spores: 16-24g metaflumizone.
Further, the proportion of metarhizium anisopliae and metaflumizone is 8 multiplied by 1000 hundred million spores: 20g of metaflumizone.
The invention also aims to protect a prodenia litura killing preparation, and the active ingredients of the prodenia litura killing preparation comprise metarhizium anisopliae and metaflumizone.
Further, the proportion of the metarhizium anisopliae and the metaflumizone is 1 multiplied by 1000 hundred million spores to 14 multiplied by 1000 million spores: 8-32g of metaflumizone.
Furthermore, the proportion of metarhizium anisopliae and metaflumizone is 4 x 1000 hundred million-12 x 1000 hundred million spores: 12-28g metaflumizone.
further, the proportion of metarhizium anisopliae and metaflumizone is 6 x 1000 hundred million-10 x 1000 hundred million spores: 16-24g metaflumizone.
Further, the proportion of metarhizium anisopliae and metaflumizone is 8 multiplied by 1000 hundred million spores: 20g of metaflumizone.
Further, the preparation is an oil suspending agent.
Further, the preparation also comprises a solvent, an emulsifier and a stabilizer.
Further, the solvent comprises vegetable oil such as rapeseed oil, sesame oil, corn oil or peanut oil.
Further, the emulsifier comprises one or more of sorbitan monooleate, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol and ethylene oxide condensate, polyoxyethylene fatty acid ester, triethanolamine monooleate, castor oil and ethylene oxide condensate.
Further, the dosage of the emulsifier is 5-10% of the total mass of the preparation.
further, the stabilizer comprises one or more of tert-butyl p-hydroxyanisole, 2, 6-di-tert-butyl p-cresol, propyl gallate, tert-butyl hydroquinone and 2-hydroxy-4-methoxybenzophenone.
further, the dosage of the stabilizer is 1% -3% of the total mass of the preparation.
further, the preparation comprises metarhizium anisopliae, metaflumizone, a solvent, an emulsifier and a stabilizer, wherein the proportion of the metarhizium anisopliae to the metaflumizone is 1 multiplied by 1000 hundred million spores to 14 multiplied by 1000 million spores: 8-32g of metaflumizone, wherein the content of the metaflumizone is 8-32% of the mass of the preparation, the dosage of the emulsifier is 5-10% of the mass of the preparation, the dosage of the stabilizer is 1-3% of the mass of the preparation, and the solvent is used for supplementing 100%.
The invention has the beneficial effects that:
The active ingredients metarhizium anisopliae and metaflumizone in the composition have obvious synergistic effect on prodenia litura.
The composition of the present invention has excellent drug efficacy.
The preparation of the invention has good quick-acting performance.
The preparation of the invention has good persistence.
The preparation of the invention is not easy to generate drug resistance for pests.
Detailed Description
the examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Indoor live test combined virulence determination test:
The toxicity of the metarhizium anisopliae CQMa421 dry spore powder, metaflumizone single agent and the composition thereof (only consisting of the metarhizium anisopliae CQMa421 dry spore powder and metaflumizone, see table 1 specifically) on prodenia litura is detected, and meanwhile, the cotoxicity coefficient is calculated, and the test results are shown in table 1.
Reagent to be tested: metarhizium anisopliae CQMa421 dry spore powder, 94% wt metaflumizone raw medicine.
A test insect source: the sensitive strain is continuously fed for multiple generations in a laboratory of the genetic engineering research center of Chongqing university.
The test method comprises the following steps:
And (3) an insect soaking method, namely soaking the third-instar spodoptera litura larvae into the liquid medicine for 5 seconds after the liquid medicine is prepared, taking out the third-instar spodoptera litura larvae, sucking off excessive water, and then putting the third-instar spodoptera litura larvae into fresh feed for feeding. Three replicates of each treatment were treated, 15 replicates each.
The mortality rate of prodenia litura is investigated 72 hours after the drug administration, and data processing is carried out to compare the activity of the test drug.
Data processing:
Calculating a virulence regression equation by using POLO Plus software, wherein the calculation parameters comprise: slope b value, standard error, kaipingSquare, degree of freedom, LC50Values and 95% confidence limits.
The combined virulence evaluation method comprises the following steps:
adopting a Sun Yunpei co-toxicity coefficient method:
Virulence index (TI) ═ LC of standard agents50LC of test agent50)×100
Actual Toxicity Index (ATI) of mixed preparation, LC for single use of A-agent50LC mixed with medicament A and B50)×100
The Theoretical Toxicity Index (TTI) of mixed agent is TI (A) x a + TI (B) x B
Wherein a represents the percentage of the medicament A in the mixture, and B represents the percentage of the medicament B in the mixture.
The cotoxicity coefficient CTC is (mixed agent actual virulence index ATI/mixed agent theoretical virulence index TTI) x 100;
According to the regulation of agricultural chemical examination institute of Ministry of agriculture, the judgment standard of the synergy of the mixture formula is as follows: the co-toxicity coefficient is more than 120, so that the synergistic effect is achieved; the cotoxicity coefficient is between 80 and 120, and is additive effect; the co-toxicity coefficient is less than 80, and the antagonism is obtained;
Except different medicaments, the treatment modes of the groups are the same; the results are shown in Table 1.
TABLE 1 toxicity test results of Metarrhizium anisopliae CQMa421 and metaflumizone single agent and its composition against Spodoptera litura
| Medicament and proportion | LC50(mg/L) | Co-toxicity coefficient CTC |
| A: metarhizium anisopliae CQMa421 | 64.56 | - |
| b: metaflumizone | 41.36 | - |
| A:B＝1:32 | 27.35 | 152.9 |
| A:B＝4:28 | 19.68 | 220.1 |
| A:B＝6:24 | 12.66 | 351.9 |
| A:B＝8:20 | 10.42 | 442.3 |
| A:B＝10:16 | 15.50 | 309.7 |
| A:B＝12:12 | 22.58 | 223.3 |
| A:B＝14:8 | 42.06 | 127.5 |
Remarking: the spore content of the used destruxins CQMa421 is 200 hundred million spores/gram, the mixture ratio in the table is simplified description, destruxins CQMa: metaflumizone ═ x: y, refers to the number of metarhizium anisopliae spores x 1000 hundred million: metaflumizone weight y/gram.
As can be seen from Table 1, the ratio of the number of spores in the metarhizium anisopliae CQMa421 dry spore powder to the gram of metaflumizone is (1-14). times.1000 hundred million spores: the metaflumizone and the metaflumizone are compounded within the range of 8-32g, the cotoxicity coefficients of the two are both greater than 120, and the two show synergistic interaction; wherein in 8 x 1000 million spores: when the amount of the metaflumizone is 20g, the synergistic effect is most obvious. Therefore, the compounding of the metarhizium anisopliae CQMa421 and the metaflumizone has a remarkable synergistic effect on the prodenia litura.
Example 1
The preparation comprises the active ingredients of metarhizium anisopliae CQMa421 and metaflumizone, wherein the content of metarhizium anisopliae dry spore powder is 10 hundred million/g, the content of metaflumizone is 32 percent by weight, and the number of metaflumizone spores is as follows: metaflumizone weight is 1 x 1000 hundred million spores: 32g metaflumizone; adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
The preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
Example 2
The preparation comprises the active ingredients of metarhizium anisopliae CQMa421 and metaflumizone, wherein the content of metarhizium anisopliae dry spore powder is 40 hundred million/g, the content of metaflumizone is 28 percent by weight, and the number of metaflumizone spores is as follows: metaflumizone weight was 4 × 1000 hundred million spores: 28g of metaflumizone; adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
The preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
Example 3
The preparation comprises the active ingredients of metarhizium anisopliae CQMa421 and metaflumizone, wherein the content of metarhizium anisopliae dry spore powder is 60 hundred million/g, the content of metaflumizone is 24 percent by weight, and the number of metaflumizone spores is as follows: metaflumizone weight is 6 x 1000 hundred million spores: 24g metaflumizone; adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
the preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
Example 4
The preparation comprises the active ingredients of metarhizium anisopliae CQMa421 and metaflumizone, wherein the content of metarhizium anisopliae dry spore powder is 80 hundred million/g, the content of metaflumizone is 20 percent by weight, and the number of metaflumizone spores is as follows: metaflumizone weight is 8 x 1000 hundred million spores: 20g of metaflumizone; adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
The preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
Example 5
The preparation comprises the active ingredients of metarhizium anisopliae CQMa421 and metaflumizone, wherein the content of metarhizium anisopliae dry spore powder is 100 hundred million/g, the content of metaflumizone is 16 percent by weight, and the number of metaflumizone spores is as follows: metaflumizone weight is 10 x 1000 hundred million spores: 16g metaflumizone; adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
the preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
Example 6
The preparation comprises the active ingredients of metarhizium anisopliae CQMa421 and metaflumizone, wherein the content of metarhizium anisopliae dry spore powder is 120 hundred million/g, the content of metaflumizone is 12 percent by weight, and the number of metaflumizone spores is as follows: metaflumizone weight is 12 x 1000 hundred million spores: 12g metaflumizone; adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
The preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
Example 7
The preparation comprises the active ingredients of metarhizium anisopliae CQMa421 and metaflumizone, wherein the content of metarhizium anisopliae dry spore powder is 140 hundred million/g, the content of metaflumizone is 8 percent by weight, and the number of metaflumizone spores is as follows: metaflumizone is 14 x 1000 hundred million spores in weight: 8g of metaflumizone; adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
The preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
Example 8
The single preparation of metarhizium anisopliae comprises the active component of metarhizium anisopliae CQMa421, wherein the content of the metarhizium anisopliae dry spore powder is 80 hundred million/g. Adding 6 wt% of sorbitan fatty acid ester polyoxyethylene ether, 3 wt% of tert-butyl hydroquinone, 1 wt% of bentonite and 0.8 wt% of white carbon black, and using salad oil to complement 100%, wherein the percentages are calculated by mass percentage of the total mass of the preparation.
The preparation method of the preparation comprises the following steps:
Mixing all the components except the metarhizium anisopliae spores, uniformly mixing the components by high-speed shearing, sanding the mixture for 2 to 3 hours by a sand mill to ensure that the average particle size reaches 1 to 5 microns, adding the metarhizium anisopliae dry spore powder, and uniformly mixing the mixture to obtain the preparation.
The preparation method of the preparation comprises the following steps:
Mixing all the components, shearing at high speed, mixing, and sanding with a sand mill for 2-3 hr to obtain a preparation with average particle diameter of 1-5 μm.
Indoor compatibility test
Placing the preparation samples of examples 1-8 in an incubator at 37 ℃ for incubation, detecting the spore germination rate at 3d and 14d respectively, and determining whether metaflumizone has influence on the germination of metarhizium anisopliae spores;
The spore germination rate detection method comprises the following steps: each preparation sample was diluted with sterile water to a spore content of 1X 107taking 50uL spore suspension, uniformly coating the suspension on a 1/4SDAY plate, and detecting the germination rate after culturing for 14h at 28 ℃;
the above groups have the same management mode except different medicaments; the results are shown in Table 2.
TABLE 2 germination Rate results
| Sample (I) | Germination rate after 3d | germination rate after 14 days |
| Example 1 | 89.9％Aa | 70.3％Aa |
| Example 2 | 87.1％Aa | 69.1％Aa |
| example 3 | 88.5％Aa | 66.0％Aa |
| Example 4 | 83.7％Aa | 67.3％Aa |
| Example 5 | 85.8％Aa | 69.5％Aa |
| Example 6 | 89.0％Aa | 70.1％Aa |
| Example 7 | 87.1％Aa | 67.9％Aa |
| Example 8 | 84.2％Aa | 66.5％Aa |
remarking: lower case differences indicate significant differences at the 0.05 level and upper case differences indicate significant differences at the 0.01 level.
As can be seen from Table 2, the germination rates of the preparations of examples 1-7 were not significantly different from that of the preparation of example 8 (i.e., metarhizium anisopliae single-dose preparation). Therefore, the compatibility of metaflumizone and metarhizium anisopliae CQMa421 is good.
and (3) field application test: cabbage prodenia litura field test
A control experiment of cabbage spodoptera litura field control was conducted using the formulations of examples 1 to 8 and 24% metaflumizone SC (afardi, produced by basf, germany) while setting a blank control group (treated with clear water);
the method specifically comprises the following steps: the medicine is applied to 2-instar larvae of prodenia litura for 1 time; repeating the treatment for three times, wherein the area of each cell is 15 square meters, and the cells are arranged in random blocks;
Investigating the number of the spodoptera litura live insects before and after the drug for 3, 7 and 21 days; and (4) performing site-specific survey on 50 cabbages in each cell according to a five-point sampling method, surveying the number of all larvae, calculating the control effect and performing variance analysis.
and (3) calculating the control effect according to the following formula:
In the formula, PT0Indicates before administration, PT1After administration, PT represents the drug treatment group, and CK represents the blank control group;
Except different medicaments, the treatment modes of the groups are the same; the results are shown in Table 3.
TABLE 3 cabbage prodenia litura field test results
Remarking: the spore content of the used destruxins CQMa421 is 200 hundred million spores/gram, the mixture ratio in the table is simplified description, and the destruxins CQMa421 (A): metaflumizone (B) ═ x: y, refers to the number of metarhizium anisopliae spores x 1000 hundred million: metaflumizone weight y/gram. The difference in lower case letters indicates a significant difference at the 0.05 level, and the difference in upper case letters indicates a significant difference at the 0.01 level; the application amount of each treatment is converted into the raw drug cost which is consistent, and the raw drug cost is set to be 3 yuan/15L of water.
As can be seen from Table 3, the synergistic effect is obvious after the components are mixed according to the proportion under the same cost, and the control effect on the cabbage prodenia litura is obviously superior to that of a single agent. Therefore, the pesticide disclosed by the invention has an excellent effect of preventing and treating prodenia litura, and is good in quick-acting property and long in lasting period.
furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. The composition for killing prodenia litura is characterized in that the active ingredients comprise metarhizium anisopliae and metaflumizone.
2. The composition of claim 1, wherein the metarhizium anisopliae is metarhizium anisopliae CQMa 421.
3. The composition of claim 1 or 2, wherein the ratio of metarhizium anisopliae to metaflumizone is 1 x 1000 million spores to 14 x 1000 million spores: 8-32g of metaflumizone.
4. The composition as claimed in claim 3, wherein the ratio of metarhizium anisopliae and metaflumizone is 8 x 1000 hundred million spores: 20g of metaflumizone.
5. The preparation for killing prodenia litura is characterized in that active ingredients comprise metarhizium anisopliae and metaflumizone.
6. The preparation of claim 5, wherein the ratio of metarhizium anisopliae and metaflumizone is 1 x 1000 million spores to 14 x 1000 million spores: 8-32g of metaflumizone.
7. The preparation of claim 6, wherein the ratio of metarhizium anisopliae and metaflumizone is 8 x 1000 hundred million spores: 20g of metaflumizone.
8. The formulation according to any one of claims 5 to 7, further comprising a solvent, an emulsifier and a stabilizer.
9. The formulation of claim 8, wherein the amount of emulsifier is 5% to 10% of the total mass of the formulation.
10. the preparation of claim 8, comprising metarhizium anisopliae, metaflumizone, a solvent, an emulsifier and a stabilizer, wherein the ratio of metarhizium anisopliae to metaflumizone is 1 x 1000 million spores to 14 x 1000 million spores: 8-32g of metaflumizone, wherein the content of the metaflumizone is 8-32% of the mass of the preparation, the dosage of the emulsifier is 5-10% of the mass of the preparation, the dosage of the stabilizer is 1-3% of the mass of the preparation, and the solvent is used for supplementing 100%.
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Application publication date: 20191213 |