CN110419441B

CN110419441B - Method for increasing total number of single ears of two-line hybrid early indica rice

Info

Publication number: CN110419441B
Application number: CN201910771160.9A
Authority: CN
Inventors: 俞法明; 张小明; 朱国富; 叶胜海; 翟荣荣; 叶靖
Original assignee: Zhejiang Academy of Agricultural Sciences
Current assignee: Zhejiang Academy of Agricultural Sciences
Priority date: 2019-08-21
Filing date: 2019-08-21
Publication date: 2021-04-20
Anticipated expiration: 2039-08-21
Also published as: CN110419441A

Abstract

The invention relates to the technical field of rice breeding, in particular to a method for improving the total single-ear grain number of two-line hybrid early-indica rice, which comprises the steps of hybridizing two-line sterile lines carrying photo-thermo-sensitive genic male sterile genes with the existing female parent, taking 2-4 cluster-type early-indica rice with the growth period not longer than 2 days of the female parent as a male parent, carrying out cluster-type two-line sterile line breeding by generations by taking the photo-thermo-sensitive genic male sterile genes and the 2-4 cluster-type characters carrying the photo-thermo-sensitive genic male sterile genes and the top small ears as breeding targets, and then selecting normal single-grain-grown conventional early-indica rice as the male parent to prepare a two-line hybrid early-indica rice combination.

Description

Method for increasing total number of single ears of two-line hybrid early indica rice

Technical Field

The invention relates to the technical field of rice breeding, in particular to a method for increasing the total number of single ears of two-line hybrid early indica rice.

Background

Hybrid rice breeding is a breeding technical means for improving the yield of rice by utilizing the heterosis of rice, and at present, two methods, namely a three-line hybrid method and a two-line hybrid method, are mainly adopted. Compared with a two-line hybridization method, the three-line hybridization method has relatively complex breeding procedures and production links, long breeding new combination period, low efficiency, multiple seed production links, low speed, high cost and high price; compared with the three-line hybridization method, the two-line hybridization method has the biggest advantages of no need of a maintainer line, simple seed production procedure, free matching and combination, high utilization rate of rice seed resources and high probability of selecting excellent combinations.

The trait of rice panicle is an important factor influencing the rice yield, and is one of the most concerned traits in rice breeding and production, and particularly, the number of grains per panicle is a key factor influencing the rice yield. The cluster rice has the characteristic of large ears and large grains, and is favorable for developing large-ear and heavy-ear rice and quickly improving the yield per unit of rice. At present, a method for improving the total number of single-ear total grains of two-line hybrid early indica rice by using clustered-ear early indica rice resources as breeding parents of the two-line hybrid method and a variety of the method are not reported.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a method for increasing the total number of single ears of two-line hybrid early indica rice.

A method for increasing the total number of single ears of two-line hybrid early indica rice is characterized in that the existing two-line sterile line carrying photo-thermo-sensitive genic male sterile genes is taken as a female parent, 2-4 clusters of top small ears with the growth period not longer than 2 days of the female parent and more than 130 clusters of average total number of single ears are taken as male parents to carry out the combination preparation of the two-line hybrid early indica rice, and the obtained two-line hybrid early indica rice combination shows the characteristic characteristics of partial clustering of top small ears by 2, more total number of single ears, normal level of thousand grain weight and setting rate, high yield and the like.

Preferably, after the two-line hybrid early indica rice is prepared in a combined manner, 2-4 cluster type photo-thermo-sensitive genic two-line sterile lines with cluster type are bred as female parents, normal single-grain-seeded conventional early indica rice is selected as male parents to carry out preparation of the two-line hybrid early indica rice combination, and the obtained two-line hybrid early indica rice combination shows the characteristic characteristics of partial cluster generation of 2 clusters with top ears, more total single-grain grains, thousand-grain weight, normal level of setting rate, high yield and the like;

preferably, the existing two-line sterile line with good combining ability and carrying the photo-thermo-sensitive genic male sterile gene is one of Hunan trial No. 253 strain 1S bred by the agricultural department in the shouxian city of Hunan province, 2008049 Hung 628S bred by the science research institute of the Hunan Yahua variety industry, and 2011050 Hung 750S bred by the science research institute of the Hunan Yahua variety industry;

preferably, the conventional early indica rice which has the growth period not longer than 2 days of the female parent and has 2-4 clusters of top small ears and the average total single ear number of more than 130 is rice resource bred by agricultural academy of sciences in Zhejiang province;

the invention at least comprises the following beneficial effects:

(1) the two-line hybrid early indica rice obtained by the technology has the characteristics that the planted rice has 2 clusters of partial top small ears, the total grain number of single ears is obviously increased, the thousand grain weight and the setting rate are kept at normal level, the high yield is expressed and the like, and the single yield can be effectively improved;

(2) the method applies 2-4 cluster-growing early indica rice resources to the breeding of 2-4 cluster-growing photo-thermo-sensitive nuclear two-line sterile lines of the top small ears, and the bred sterile lines have the advantages of high seed reproduction and seed production yield and the like, thereby achieving the purpose of improving the seed reproduction and seed production efficiency of the two-line hybrid early indica rice;

(3) the method applies 2-4 cluster-growing early indica rice resources as male parents to the combined breeding of the two-line hybrid early indica rice, the seed reproduction of the male parents and the seed production yield of the hybrid rice are high, and the purposes of improving the seed reproduction and seed production efficiency of the two-line hybrid early indica rice can be achieved;

(4) the existing two-line sterile line with good combining ability and carrying photo-thermo-sensitive genic male sterile genes is a female parent, after two-line hybrid early indica rice combination preparation is carried out by taking 2-4 clustered small top ears with the growth period not longer than 2 days of the female parent and conventional early indica rice with the average total single ear number of more than 130 as a male parent, 2-4 clustered photo-thermo-sensitive genic male sterile lines with the top small ears are bred as the female parent, and normal single-grain-bearing conventional early indica rice is selected as the male parent to carry out the preparation of the two-line hybrid early indica rice combination, so that hybrid progeny has rich genetic basis, and the single yield is effectively improved on the basis of keeping cluster property.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Drawings

FIG. 1 is a schematic view of the steps of the embodiment 1;

FIG. 2 is a schematic view of the steps of the embodiment 2;

Detailed Description

The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.

In order to more clearly illustrate the technical solution of the present invention, the following specific examples are further illustrated.

Example 1

(1)2 cluster-growing early indica rice Zhe 17104 (Zhejiang agricultural academy of sciences, purchased from Zhejiang agricultural academy of sciences) with top small ears are selected as male parents. Zhe 17104 characteristic: the whole growth period is about 110 days, the plant height is about 89.3cm, the effective spike per mu is about 21.6 ten thousand, the spike length is about 16.5cm, and 2 grains of rice are grown on the tops of each small branch; the total grain number of each ear is about 133.4 grains, the solid grain number of each ear is about 109.5 grains, the setting percentage is about 82.1 percent, the weight of thousand grains is about 25.8g, the grain shape is oval, and the rice quality is suitable for processing and other purposes.

(2) Xiangling 628S is selected as the female parent of the two-line sterile line hybrid.

(3) Two lines of early hybrid indica rice combination "Ling Liang you 17014" (Xiang Ling 628S XZhe 17104) are hybridized. The two-line hybrid early indica rice combination 'Ling Liangyou 0516' (Zhe Ju rice 2014004, Xiang Ling 628S x 05YP16) of the same female parent is used as a contrast to carry out the identification of the cell yield and the agronomic characters.

The obtained two-line hybrid early indica rice combination 'Ling Liangyou 17014' shows the characteristic characteristics of partial top small ears of 2 clusters, more total single ear grains, thousand grain weight and fructification rate keeping normal level, high yield and the like:

the average yield per mu in 2018 is 517.6kg, and the yield is increased by 5.1 percent compared with the reference 'Lingshangyou 0516'. The whole growth period is 108.5 days later than the reference 'Lingshangyou 0516' by 0.5 days; the plant height is 89.0cm, and is similar to that of a control; 19.5 thousands of effective spikes per mu, similar to the control; the ear length is 19.8cm, and the comparison length is 2.5 cm; 2 grains of rice are grown on the tops of part of the small branch stalks; the total number of grains per spike is 165.8 grains, which is 36.3 grains more than that of the control; the number of the solid grains of each ear is 136.8 grains, which is 28.4 grains more than that of the control; the setting rate is 82.5 percent and is 1.2 percent lower than that of a control; thousand grains weighed 25.3g, the same as the control.

Example 2

(1) In 2014, the method takes Xiangling 750S as a female parent, uses the top spikelet 2 clustered early indica rice Zhe 17104 (bred line of Zhejiang agricultural academy of sciences, purchased from Zhejiang agricultural academy of sciences) with the growth period similar to that of Xiangling 750S as a male parent to hybridize, uses the two-line sterile line breeding standard and the top spikelet 2-4 clustered characters as breeding targets, carries out the clustered two-line sterile line breeding generation by generation, and breeds the top spikelet 2 clustered two-line sterile line material 4528S.

(2) Selecting ' 4528S ' as female parent and ' 05YP16 ' (male parent of Ling-Eryou 0516 ' of conventional early indica rice bred by Zhejiang agricultural academy of sciences) as male parent.

(3) The two-line hybrid early indica rice combination '4528S x 05YP 16' is prepared by hybridization, and the two-line hybrid early indica rice combination 'Ling Liangyou 0516' of the same male parent is used as a contrast to carry out the identification of the cell yield and the agronomic characters.

The obtained two-line hybrid early indica rice combination '4528S multiplied by 05YP 16' shows the characteristic characteristics of partial top spikelet 2 clustering, large total grain number of single spike, normal level of thousand grain weight and seed setting rate, high yield and the like:

the average yield per mu in 2018 is 508.3kg, and the yield is increased by 3.2 percent compared with the reference 'Lingshangyou 0516'. The whole growth period is 108.0 days, which is similar to the reference 'Lingshangyou 0516'; the plant height is 88.7cm, which is similar to the contrast; 19.2 thousands of effective spikes per mu, similar to the control; the ear length is 17.7cm, and the comparison length is 0.4 cm; 2 grains of rice are grown on the tops of part of the small branch stalks; the total grain number of each ear is 145.1 grains which is 15.6 grains more than that of the control; the number of the solid grains of each ear is 120.3 grains, which is 11.9 grains more than that of the control; the setting rate is 82.9 percent and is 0.8 percent lower than that of a control; the thousand grains weighed 26.1g, which was 1.0g heavier than the control.

While embodiments of the invention have been disclosed above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the generic concept as defined by the claims and their equivalents.

Claims

1. A method for improving the total number of single ears of two-line hybrid early indica rice is characterized in that the existing two-line sterile line carrying photo-thermo-sensitive genic male sterile genes is taken as a female parent, and the conventional early indica rice with 2-4 cluster-grown top small ears and average total number of single ears of more than 130 grains, the growth period of which is not longer than 2 days of the female parent, is taken as a male parent to carry out the combination preparation of the two-line hybrid early indica rice to obtain the two-line hybrid early indica rice combination;

after the two-line hybrid early indica rice is prepared in a combined manner, 2-4 cluster-growing type photo-thermo-sensitive nuclear two-line sterile lines with top small ears are bred as female parents, and normal single-grain-bearing conventional early indica rice is selected as male parents to carry out preparation of the two-line hybrid early indica rice combination, so that the two-line hybrid early indica rice combination is obtained;

the conventional early indica rice male parent with 2-4 cluster-grown top small ears and average single ear total grain number of more than 130 with the growth period not longer than 2 days of the female parent is 2 cluster-grown early indica rice Zhe 17104 bred by agricultural academy of Zhejiang province.

2. The method for increasing the total number of single ears of two-line hybrid early indica rice as claimed in claim 1, wherein said two-line sterile line with photo-thermo-sensitive genic male sterile gene with better combining ability is one of Hunan trial No. 253 strain 1S bred by the agricultural department of Tazhou province city, Hunan audition rice 2008049 Hung Ling 628S bred by the Yahua species science research institute of Hunan province, and Hunan audition rice 2011050 Hung 750S bred by the Yahua species science research institute of Hunan province.