CN110342637A - A kind of device and method improving low-temperature biological denitrification efficiency - Google Patents
A kind of device and method improving low-temperature biological denitrification efficiency Download PDFInfo
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- CN110342637A CN110342637A CN201910665281.5A CN201910665281A CN110342637A CN 110342637 A CN110342637 A CN 110342637A CN 201910665281 A CN201910665281 A CN 201910665281A CN 110342637 A CN110342637 A CN 110342637A
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- 238000000034 method Methods 0.000 title claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000003864 humus Substances 0.000 claims abstract description 22
- 229920000049 Carbon (fiber) Polymers 0.000 claims abstract description 20
- 239000004917 carbon fiber Substances 0.000 claims abstract description 20
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000010865 sewage Substances 0.000 claims abstract description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910002804 graphite Inorganic materials 0.000 claims abstract description 12
- 239000010439 graphite Substances 0.000 claims abstract description 12
- 229910021607 Silver chloride Inorganic materials 0.000 claims abstract description 9
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims abstract description 9
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 6
- 230000001464 adherent effect Effects 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 230000002572 peristaltic effect Effects 0.000 claims description 7
- 230000001376 precipitating effect Effects 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 5
- 239000013049 sediment Substances 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000001257 hydrogen Substances 0.000 claims description 3
- 229910052739 hydrogen Inorganic materials 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims 1
- 239000004575 stone Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 238000012546 transfer Methods 0.000 abstract description 2
- 230000006641 stabilisation Effects 0.000 abstract 1
- 238000011105 stabilization Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229910003208 (NH4)6Mo7O24·4H2O Inorganic materials 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 229910018162 SeO2 Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- AHEWZZJEDQVLOP-UHFFFAOYSA-N monobromobimane Chemical compound BrCC1=C(C)C(=O)N2N1C(C)=C(C)C2=O AHEWZZJEDQVLOP-UHFFFAOYSA-N 0.000 description 1
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/005—Combined electrochemical biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Engineering & Computer Science (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The present invention relates to a kind of devices for improving low-temperature biological denitrification efficiency, including reactor, carbon fiber felt cathode, graphite anode, Ag/AgCl reference electrode and power supply, water inlet is equipped with below sidewall of reactor, water outlet is equipped with above the side wall of another side, graphite anode is located in the middle part of reactor, it is set in reactor within carbon fiber felt cathode adherent one week, graphite anode, carbon fiber felt cathode are connect with the anode of power supply and cathode respectively, and described device further includes the Ag/AgCl reference electrode being located in reactor；The carbon fiber felt cathode surface is fixed with one layer of solid-state humus.Using the device biological denitrification denitrogenation can be carried out to sewage, the apparatus structure is simple, electronics is provided using the cathode that solid-state humus is modified for biological denitrification, reduce the dosage of carbon source, and accelerate the electronics transfer of reaction process, the biological denitrification reaction efficiency under cryogenic conditions is improved, and solid-state Humus stabilization not easily runs off, the influence to water environment is smaller.
Description
Technical field
The present invention relates to a kind of device and methods of biological denitrification efficiency under raising cryogenic conditions, belong to sewage treatment skill
Art field.
Background technique
Activated sludge process is the mainstream technology of bio-denitrifying sewage, is mainly completed by two stages of nitrification and denitrification, ginseng
Microorganism with this process is mainly nitrifier and denitrifying bacterium.The study found that the reduction for temperature, denitrifying bacteria ratio
Nitrobacteria is more sensitive.Under cryogenic, denitrifying microorganism slow growth, reaction rate is extremely low, can make biological anti-
The nitrification starting time is too long, seriously affects the effect of biological denitrification denitrogenation.It is generally believed that denitrifying microorganism optimum water temperature exists
30-35 DEG C, 15 DEG C of water temperature belong to low temperature.And the most of the time in China North China, northeast, the Northwest, 1 year is in
Low temperature environment, ice period, for underground water temperature generally at 10 DEG C or so, some was even lower than 5 DEG C up to 3-6 months.Therefore, always
Since, low temperature is the restrictive factor of nitrate-N pollution bioremediation of waters (biological denitrification) success or failure.
Traditional solution mainly has optimization denitrification process technological parameter (additional carbon concentration, dissolved oxygen concentration, water
Power residence time etc.), add Psychrotrophs (be added directly, immobilization after add), add solid packing to biofilm reactor
(IFAS, MBBR etc.), though can improve the effect of denitrification denitrogenation to a certain extent, the degree on the one hand improved has
Limit, on the other hand, there is also energy consumption and it is at high cost the problems such as.Therefore, it is raw that a kind of reinforcing cryogenic conditions that new cost is low are developed
The denitrifying method of object is extremely urgent.
Summary of the invention
It is an object of the invention to solve the deficiency in above-mentioned background technique, a kind of raising low-temperature biological denitrification effect is provided
The device and method of rate, the apparatus structure is simple, denitrifying bacterium can be attached to the cathode surface of solid-state humus modification, low
Quick start biological denitrification reacts under the conditions of temperature, can greatly improve the denitrification efficiency of microorganism under cryogenic conditions, method letter
It is single, it is at low cost.
Technical scheme is as follows:
A kind of device improving low-temperature biological denitrification efficiency, including reactor, carbon fiber felt cathode, graphite anode, Ag/
AgCl reference electrode and power supply, sidewall of reactor lower section are equipped with water inlet, are equipped with water outlet above the side wall of reactor another side,
Graphite anode is located in the middle part of reactor, is set in reactor within carbon fiber felt cathode adherent one week, the graphite anode, carbon fiber felt
Cathode is connect with the anode and cathode of power supply respectively, and described device further includes the Ag/AgCl reference electrode being located in reactor；Institute
It states carbon fiber felt cathode surface and is fixed with one layer of solid-state humus.
Further, the reactor is the organic glass reactor of tubular.
Further, the solid-state humus with a thickness of 0.5mm, solid-state humus is fixed on carbon fiber felt yin by carbon paste
Pole surface.
Further, the preparation method of the solid-state humus, includes the following steps:
(1) 100g soil sample is taken, after air-drying, the HF of 150mL 2% is added, is placed on after being shaken for 24 hours on shaking table, be centrifuged, go
Fall supernatant, this step is repeated 2 times, and collects precipitating；
(2) NaOH solution of 0.1mol/L is added into the precipitating of collection, is placed on after being shaken for 24 hours on shaking table, is centrifuged, receives
Collect lower sediment, this step repeats at least 10 times；
(3) it is added in the lower sediment obtained to step (2) HF washing 2 times of 2%, then with distilled water flushing in
Property, it is finally freeze-dried, obtains solid-state humus.
The method for carrying out biological denitrification denitrogenation to sewage using above-mentioned apparatus, includes the following steps:
(1) with river bottom mud for original inoculation source, Anaerobic culturel is carried out, the reduction amount of nitrate nitrogen is tested after 10 days, it will
Active culture continuous passage 3 times or more, obtain denitrification strain；
(2) it is inoculated with denitrifying bacterium kind in the reactor of aforementioned device, is led in reactor using peristaltic pump from water inlet
Enter treatment sewage, is then carried out under conditions of constant cathode electrode potential is -500mV (relative to standard hydrogen electrode) static
Cathode biofilm, the revolving speed by adjusting peristaltic pump control flow, make sewage at hydraulic retention 15-20 hours of inside reactor,
And sewage pH6.9-7.2 is maintained, the water after denitrogenation is discharged from the water outlet of reactor.
Further, in step (1), the culture medium of Anaerobic culturel forms (g/L) are as follows: NaNO30.425, CH3COONa
0.820, Na2HPO41.920 NaH2PO40.460, KCl 0.050.
Further, in step (2), (volume/reactor of inoculation liquid effectively holds the inoculum concentration 30v% of denitrification strain
Product).
The present invention has the advantage that
(1) the device of the invention structure is simple, provides electronics using the cathode that solid-state humus is modified for biological denitrification,
Reduce the dosage of carbon source, and accelerate the electronics transfer of reaction process, improves the biological denitrification reaction under cryogenic conditions
Efficiency.
(2) method of the present invention is easy to operate, and solid-state Humus is stable and not easily runs off, to the shadow of water environment
Sound is smaller.
(3) present invention can be with the nitrate in the removal low-temperature sewage of economical and efficient.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for the device that the present invention improves low-temperature biological denitrification efficiency；
Wherein, 1- reactor；2- carbon fiber felt cathode；3- graphite anode；4-Ag/AgCl reference electrode；5- power supply；6- is solid
State humus；
Specific embodiment
The present invention will be further explained below with reference to the attached drawings and specific examples, but protection scope of the present invention not office
It is limited to this.In following embodiments, the peristaltic pump used is purchased from Baoding LanGe constant flow pump Co., Ltd；Model is BT100-1F/DG-
4, potentiostat is purchased from Shanghai Chen Hua Instrument Ltd., and model is CHI1000C；Electrode, inlet and outlet pipes set for regular market purchase
It is standby.
Embodiment 1
Such as Fig. 1, a kind of device improving low-temperature biological denitrification efficiency, including reactor 1, carbon fiber felt cathode 2, graphite
Anode 3, Ag/AgCl reference electrode 4 and power supply 5, reactor 1 are the organic glass reactor of tubular, are set below 1 side wall of reactor
There is water inlet, water outlet is equipped with above the side wall of 1 another side of reactor, graphite anode 3 is located in the middle part of reactor, carbon fiber felt yin
Pole is set in reactor 1 for 2 adherent one weeks, and the graphite anode 3, carbon fiber felt cathode 2 connect with the anode of power supply 5 and cathode respectively
It connects, described device further includes the Ag/AgCl reference electrode 4 being located in reactor；2 surface of carbon fiber felt cathode is fixed with one
Layer solid-state humus 6, the solid-state humus with a thickness of 0.5mm, it is negative that solid-state humus by carbon paste is fixed on carbon fiber felt
Pole surface.
The preparation method of the solid-state humus, includes the following steps:
(1) 100g soil sample is taken, after air-drying, the HF of 150ml 2% is added, is placed on after being shaken for 24 hours on shaking table, be centrifuged, go
Fall supernatant, this step is repeated 2 times, and collects precipitating；
(2) NaOH solution of 0.1mol/L is added into the precipitating of collection, is placed on after being shaken for 24 hours on shaking table, is centrifuged, receives
Collect supernatant and lower sediment, this step repeats at least 10 times；
(3) it is added in the lower sediment obtained to step (2) HF washing 2 times of 2%, then with distilled water flushing in
Property, it is finally freeze-dried, obtains solid-state humus.
Embodiment 2
The method for carrying out biological denitrification denitrogenation to sewage using the device of embodiment 1, includes the following steps:
(1) with river bottom mud (bed mud picks up from Nanjing south of a city river) for original inoculation source, Anaerobic culturel (10g bed mud is carried out
It is added in the anaerobism bottle (60mL) containing 20mL culture medium and cultivates, the composition (g/L) of culture medium is NaNO30.425,
CH3COONa 0.820, Na2HPO41.920 NaH2PO40.460, KCl 0.050) reduction of nitrate nitrogen, is tested after 10 days
Amount, by active culture continuous passage 3 times or more, obtains denitrification strain；
(2) aforementioned device is used, the inoculation denitrifying bacterium kind (inoculum concentration 30v% (inoculation of denitrification strain in reactor
The volume of liquid/reactor dischargeable capacity)), it is passed through manual simulation's nitrate nitrogen dirt in reactor from water inlet using peristaltic pump
(sewage forms (g/L): NaNO to water3 0.106,CH3COONa 0.123,NaHCO3 0.084,Na2HPO4 0.350,NaH2PO4
0.170,KCl 0.150,MgSO4·7H2O 0.150,CaCl2 0.030,FeSO4·7H2O 0.002,1mL microelement
(MnCl2·4H2O 0.900,ZnSO4·7H2O 0.430,NiCl2·6H2O 0.200,H3BO3 0.014,CoCl2·6H2O
0.240,CuSO4·5H2O 0.250,(NH4)6Mo7O24·4H2O 0.200,SeO20.050)), in 10 DEG C of insulating box,
Static cathode biofilm is carried out under conditions of constant cathode electrode potential is -500mV (relative to standard hydrogen electrode), passes through adjusting
The revolving speed of peristaltic pump controls flow, makes sewage at hydraulic retention 15-20 hours of inside reactor, and maintain sewage pH6.9-
7.2, the water after obtaining denitrogenation.
Experiment measures, and after reactor is run 40 days, cathode biofilm development is mature, has successfully started up low-temperature biological denitrification
Reaction can efficiently remove the nitrate nitrogen in low-temperature sewage, and reactor enters the relatively stable operation phase, at this time denitrification
Rate can achieve 15-22mgNO3 -- N/L/d, and the denitrification rate that solid-state humus no applied voltage is not added is only 5-
11mgNO3 --N/L/d.This illustrates that the device of the invention improves biological denitrification efficiency under cryogenic conditions, and reactor is discharged
NO3 -The concentration of-N reaches surface water (10mgN/L, Group III) and underground water (20mgN/L, Group III) environmental quality standards.
Claims (7)
1. a kind of device for improving low-temperature biological denitrification efficiency, which is characterized in that including reactor, carbon fiber felt cathode, stone
Black anode, Ag/AgCl reference electrode and power supply, sidewall of reactor lower section are equipped with water inlet, the side wall top of reactor another side
Equipped with water outlet, graphite anode is located in the middle part of reactor, is set in reactor within carbon fiber felt cathode adherent one week, the graphite sun
Pole, carbon fiber felt cathode are connect with the anode of power supply and cathode respectively, and described device further includes the Ag/AgCl being located in reactor
Reference electrode；The carbon fiber felt cathode surface is fixed with one layer of solid-state humus.
2. improving the device of low-temperature biological denitrification efficiency as described in claim 1, which is characterized in that the reactor is tubular
Organic glass reactor.
3. improving the device of low-temperature biological denitrification efficiency as described in claim 1, which is characterized in that the solid-state humus
With a thickness of 0.5mm, solid-state humus is fixed on carbon fiber felt cathode surface by carbon paste.
4. improving the device of low-temperature biological denitrification efficiency as described in claims 1 or 2 or 3, which is characterized in that the solid-state is rotten
The preparation method for growing matter, includes the following steps:
(1) take 100g soil sample, air-dry after, be added 150mL 2% HF, be placed on after being shaken for 24 hours on shaking table, be centrifuged, remove on
Clear liquid, this step are repeated 2 times, and collect precipitating；
(2) NaOH solution of 0.1mol/L is added into the precipitating of collection, is placed on after being shaken for 24 hours on shaking table, is centrifuged, under collection
Layer precipitating, this step repeat at least 10 times；
(3) it is added in the lower sediment obtained to step (2) HF washing 2 times of 2%, then with distilled water flushing up to neutral,
It is finally freeze-dried, obtains solid-state humus.
5. the method for carrying out biological denitrification denitrogenation to sewage using any one of claim 1-4 described device, which is characterized in that
Include the following steps:
(1) with river bottom mud for original inoculation source, Anaerobic culturel is carried out, the reduction amount of nitrate nitrogen is tested after 10 days, there will be work
Culture continuous passage 3 times or more of property, obtain denitrification strain；
(2) denitrifying bacterium kind is inoculated in the reactor of aforementioned device, be passed through in reactor using peristaltic pump from water inlet to
Sewage is handled, static cathode is then carried out under conditions of constant cathode electrode potential is -500mV (relative to standard hydrogen electrode)
Biofilm, the revolving speed by adjusting peristaltic pump control flow, make sewage at hydraulic retention 15-20 hours of inside reactor, and tie up
Sewage pH6.9-7.2 is held, the water after denitrogenation is discharged from the water outlet of reactor.
6. the method for carrying out biological denitrification denitrogenation to sewage as claimed in claim 5, which is characterized in that in step (1), anaerobism
The culture medium of culture forms (g/L) are as follows: NaNO30.425, CH3COONa 0.820, Na2HPO41.920 NaH2PO40.460,
KCl 0.050。
7. the method for carrying out biological denitrification denitrogenation to sewage as described in claim 5 or 6, which is characterized in that in step (2),
The inoculum concentration 30% (v/v) of denitrification strain.
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| CN201910665281.5A CN110342637A (en) | 2019-07-23 | 2019-07-23 | A kind of device and method improving low-temperature biological denitrification efficiency |
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| CN201910665281.5A CN110342637A (en) | 2019-07-23 | 2019-07-23 | A kind of device and method improving low-temperature biological denitrification efficiency |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113088478A (en) * | 2021-03-31 | 2021-07-09 | 南京工业大学 | Method for improving activity of denitrification type anaerobic methane oxidation microorganisms |
| CN115124187A (en) * | 2022-05-25 | 2022-09-30 | 温州大学 | Method for treating high-nitrate-nitrogen pickling wastewater based on watermelon peel |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030131641A1 (en) * | 2000-04-24 | 2003-07-17 | Shulgin Alexandr Ivanovich | Method for producing a humin-mineral concentrate and device for carrying out said method |
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| CN104787977A (en) * | 2015-04-17 | 2015-07-22 | 湖南大学 | Continuous flow integrated electrode bio-membrane reactor and nitrate removal technology |
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| CN113088478A (en) * | 2021-03-31 | 2021-07-09 | 南京工业大学 | Method for improving activity of denitrification type anaerobic methane oxidation microorganisms |
| CN115124187A (en) * | 2022-05-25 | 2022-09-30 | 温州大学 | Method for treating high-nitrate-nitrogen pickling wastewater based on watermelon peel |
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