CN110333341A - A method of silk fabric cultural relics sample is identified based on protein biochip technology - Google Patents
A method of silk fabric cultural relics sample is identified based on protein biochip technology Download PDFInfo
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 24
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 24
- 239000004744 fabric Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000000018 DNA microarray Methods 0.000 title claims abstract description 14
- 239000002096 quantum dot Substances 0.000 claims abstract description 27
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 20
- 241000283707 Capra Species 0.000 claims abstract description 11
- 229960003180 glutathione Drugs 0.000 claims abstract description 11
- 239000000427 antigen Substances 0.000 claims abstract description 10
- 102000036639 antigens Human genes 0.000 claims abstract description 10
- 108091007433 antigens Proteins 0.000 claims abstract description 10
- 108010022355 Fibroins Proteins 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 32
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 10
- 108010024636 Glutathione Proteins 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- YASYEJJMZJALEJ-UHFFFAOYSA-N Citric acid monohydrate Chemical compound O.OC(=O)CC(O)(C(O)=O)CC(O)=O YASYEJJMZJALEJ-UHFFFAOYSA-N 0.000 claims description 7
- 229910004273 TeO3 Inorganic materials 0.000 claims description 7
- 229960002303 citric acid monohydrate Drugs 0.000 claims description 7
- 238000010992 reflux Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000013642 negative control Substances 0.000 claims description 5
- 230000001376 precipitating effect Effects 0.000 claims description 5
- 239000012279 sodium borohydride Substances 0.000 claims description 5
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- YKYOUMDCQGMQQO-UHFFFAOYSA-L cadmium dichloride Chemical compound Cl[Cd]Cl YKYOUMDCQGMQQO-UHFFFAOYSA-L 0.000 claims description 4
- 239000012452 mother liquor Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 2
- 235000003969 glutathione Nutrition 0.000 abstract description 9
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract description 3
- 239000008346 aqueous phase Substances 0.000 abstract description 2
- 238000005253 cladding Methods 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 230000002194 synthesizing effect Effects 0.000 abstract description 2
- 238000012795 verification Methods 0.000 abstract description 2
- 238000003491 array Methods 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 239000004753 textile Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 238000003760 magnetic stirring Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 238000006424 Flood reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- -1 amino, carboxyl Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009954 braiding Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/36—Textiles
- G01N33/365—Filiform textiles, e.g. yarns
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- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Treatment Of Fiber Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to verification retrieval fields, disclose a kind of method for identifying silk fabric cultural relics sample based on protein biochip technology, capture antibody of the present invention using quantum dot-labeled goat anti-rabbit antibodies of the surface with carboxyl of Aqueous phase synthesizing glutathion cladding as protein-chip, the protein of historical relic sample is extracted as antigen, rabbit-anti fibroin albumen antibody is as sessile antibody, according to the fluorescence signal of generation, qualitatively detect silk fabric cultural relics sample, this method has high throughput, the properties such as high specific, high sensitivity.
Description
Technical field
The present invention relates to verification retrieval fields, more particularly to a kind of protein biochip technology that is based on to identify silk fabric cultural relics sample
Method.
Background technique
In immense historical relic, textile historical relic is wide in variety, and braiding style is beautiful absolutely, comfortable ventilative, is very valuable
Cultural heritage, wherein most prestigious with silk fabrics, in Western Han Dynastry's period before more than 2,000 years, our ancestors just open connection
The land channel of oriental and occidental cultures, that is, famous " Silk Road ".The Silk Road promotes the cultural exchanges of Chinese and western.Edge
This Silk Road, the China Textile of magnificent brilliance traveled to Europe, its rule of not only having taken is beautiful
Textile, ancient the eastern civilization of also having taken.Research textile historical relic has research Ancient Times in China politics, economy and culture
Very important meaning.
However, the ground due to silk fabric cultural relics is relatively fragile, in very long historical floods, these silk fabric cultural relics are just very
It is easy to be influenced by ambient light, heat, oxygen, ray, microorganism etc. and degradation with aging occurs, causes naked eyes that can not identify.This is just
So that we can not obtain more information to study ancient civilization.Therefore, accurately and efficiently identify Ancient Silk Textile for chasing after
The silk that traces back origin and research human civilization have critically important meaning.
Summary of the invention
In order to solve the above-mentioned technical problems, the present invention provides one kind to identify silk fabric cultural relics based on protein biochip technology
The method of sample.
The specific technical proposal of the invention is: a kind of method for identifying silk fabric cultural relics sample based on protein biochip technology,
The following steps are included:
1) the historical relic sample of 0.01g-0.1g is dissolved in 9.6 buffer of CB of 100-1000mL, is mixed evenly,
It stands, taking supernatant is antigen liquid.
2) by CdCl2·2.5H2O is dissolved in deionized water, and glutathione, two citric acid monohydrate trisodiums, Na is added2TeO3
And NaBH4, under stirring, pH is adjusted with NaOH, acquired solution is transferred in the microwave device with reflux, when solution face
Color starts the 2-3h that flows back when being in light green, obtain quantum dot.
3) quantum dot is added to absolute ethanol, 3-7min is centrifuged at 3500-4500rpm, remove supernatant, so weight
It is 2-4 times multiple, precipitating is resuspended in PBS 7.4.
4) quantum dot mother liquor obtained by 20-30 μ L step 3) is taken, 18-22 μ L EDC solution and the mixing of 8-12 μ L NHS solution are anti-
Answer 3-7min；0.15-0.25mg goat anti-rabbit antibodies are added, 2-4h is reacted under room temperature, obtain quantum dot mark after reaction
The goat anti-rabbit antibodies of note are secondary antibody liquid, are placed at 1-5 DEG C and save backup.
5) rabbit-anti fibroin albumen antibody is made to the antibody fixer of 0.4-0.6mg/mL of protein spots sample liquid, uses albumen
The spotting needle of chip point sample instrument carries out point sample on PSG, and point sample terminates to be placed in chip fixed bin, fixed at 35-39 DEG C
18-19h。
6) after fixed, 30-40 microlitres of 0.8-1.2wt%BSA solution is added into the array of gained substrate, as
Confining liquid closes 1.5-2.5h at 35-39 DEG C.
7) confining liquid is discarded, drying after being washed 4-6 times with PBS 7.4 takes obtained by step 1) 30-40 microlitres of antigen liquid, adds
Enter array, using 9.6 buffer of CB as negative control, is incubated for 0.5-1.5h under conditions of 35-39 DEG C.
8) antigen liquid is discarded, drying after being washed 4-6 times with PBS 7.4 takes secondary antibody liquid obtained by 30-40 microlitres of step 4) to be added
Array is incubated for 0.5-1.5h under conditions of 35-39 DEG C.
9) secondary antibody liquid is discarded, after being washed 4-6 times with PBS 7.4 after drying, is believed using Fluorescence Scanner Scanning Detction fluorescence
Number value, if historical relic sample be silk fabrics, can detect fluorescence signal, on the contrary then nothing.
Preferably, in step 2), the preparation method of the quantum dot specifically: by 2.5 × 10-4The CdCl of mol2·
2.5H2O is dissolved in 25mL deionized water, is added 3.0 × 10-4Mol glutathione, 0.1g two citric acid monohydrate trisodiums, 0.5 ×
10-4mol Na2TeO3With 2.5 × 10-4mol NaBH4, under stirring, pH to 10-11 is adjusted with NaOH, is put into band reflux
Microwave device in, start when solution colour is just in light green flow back 2-3h.
Because glutathione is per se with the water soluble functional groups such as mercapto functional group and amino, carboxyl, and and Cd2+With good
Good chelation, therefore quantum dot is wrapped up using glutathione.
Preferably, the concentration of the quantum dot mother liquor is 0.8-1.2mg/mL, used EDC solution in step 4)
Concentration is 8-12mg/mL, and NHS solution concentration is 1.3-1.7mg/mL, activates the carboxyl of quantum dot surface.
Preferably, step 7), 8) and 9) in, be 4-6min/ time with the washing of PBS 7.4, it is intended to wash off unbonded resist
The substances such as original antibody avoid generating interference to experimental result.
It is compared with the prior art, the beneficial effects of the present invention are:
(1) present invention is good using the quantum dot yield high stability of Aqueous phase synthesizing glutathion cladding, and surface is rich
Carboxyl is high with the Percentage bound of antibody.
(2) in the present invention, quantum dot can overcome different traditional organic dyestuff to need different excitation wavelengths, nothing
The shortcomings that method achievees the purpose that while detecting, excitation wavelength range is wide, therefore can excite difference with an excitation light source
The quantum dot of size.
(3) present invention detects silk fabric cultural relics sample using protein biochip technology, has high throughput, high specific, Gao Ling
The properties such as sensitivity.
Specific embodiment
The present invention will be further described with reference to the examples below.
Embodiment 1
A method of silk fabric cultural relics sample is identified based on protein biochip technology, comprising the following steps:
1) seven kinds of 0.01g different historical relic samples are dissolved in 9.6 buffer of CB of 100ml, are mixed evenly, it is quiet
It sets.
2) by CdCl in 250ml three neck round bottom flask2·2.5H2O(2.5×10-4Mol) it is dissolved in 25ml deionized water
In, glutathione (3.0 × 10 is added-4Mol), two citric acid monohydrate trisodiums (0.1g), Na2TeO3(0.5×10-4Mol) and
NaBH4(2.5×10-4Mol), under stirring, pH to 10 is adjusted with NaOH, be put into the microwave device with reflux, when molten
Liquid color starts the 2h that flows back when being just in light green.
3) dehydrated alcohol is added in the quantum dot selected, and 5min is centrifuged under conditions of 3500rpm, removes supernatant, so
It is repeated 2 times, precipitating is resuspended in PBS 7.4.
4) 25 μ L quantum dot mother liquors (1mg/ml), 20 μ L EDC solutions (10mg/mL) and 10 μ L NHS (1.5mg/ml) are taken
Solution hybrid reaction about 5min.0.2mg goat anti-rabbit antibodies are added, totally 100 μ L marker, reacts 2h under room temperature.Marker
It sets in magnetic stirring apparatus and mixes, make its reaction uniformly, be placed in 4 DEG C of refrigerators after reaction and save.
5) rabbit-anti fibroin albumen antibody is made to the antibody fixer of 0.5mg/ml of protein spots sample liquid, uses protein chip
The spotting needle of point sample instrument carries out point sample on PSG, and point sample terminates to be placed in chip fixed bin, fixes 18h at 37 DEG C.
6) after fixed, 30 microlitres of 1%BSA solution is added in every array of substrate, closes 2h under conditions of 37 DEG C.
7) confining liquid is discarded, 4 times (5min/ times) is washed with PBS 7.4 and dries afterwards, takes step 1) treated seven kinds of differences
Each 30 microlitres of supernatant, different arrays is separately added into, using 9.6 buffer of CB as negative control, under conditions of 37 DEG C
It is incubated for 1h.
8) antigen liquid is discarded, 4 times (5min/ times) is washed with PBS 7.4 and dries afterwards, takes step 4) treated quantum dot mark
The goat anti-rabbit antibodies of note are separately added into different arrays, and 30 microlitres of every array is incubated for 1h under conditions of 37 DEG C.
9) secondary antibody liquid is discarded, after being dried afterwards with the washing of PBS 7.4 4 times (5min/ times), uses Fluorescence Scanner Scanning Detction
Fluorescence signal value can detect fluorescence signal if historical relic sample is silk fabrics, on the contrary then do not have.
Embodiment 2
A method of silk fabric cultural relics sample is identified based on protein biochip technology, comprising the following steps:
1) seven kinds of 0.055g different historical relic samples are dissolved in 9.6 buffer of CB of 550mL, are mixed evenly, it is quiet
It sets.
2) by CdCl in 250ml three neck round bottom flask2.2.5H2O(2.5×10-4Mol it) is dissolved in 25ml deionized water,
Glutathione (3.0 × 10 is added-4Mol), two citric acid monohydrate trisodiums (0.1g), Na2TeO3(0.5×10-4) and NaBH mol4
(2.5×10-4Mol), under stirring, pH to 10.5 is adjusted with NaOH, be put into the microwave device with reflux, when solution face
Color starts the 2.5h that flows back when being just in light green.
3) dehydrated alcohol is added in the quantum dot selected, and 5min is centrifuged under conditions of 4000rpm, removes supernatant, so
It is repeated 3 times, precipitating is resuspended in PBS 7.4.
4) 25 μ L quantum dot mother liquors (1mg/ml), 20 μ L EDC solutions (10mg/mL) and 10 μ L NHS (1.5mg/ml) are taken
Solution hybrid reaction about 5min.0.2mg goat anti-rabbit antibodies are added, totally 100 μ L marker, reacts 3h under room temperature.Marker
It sets in magnetic stirring apparatus and mixes, make its reaction uniformly, be placed in 4 DEG C of refrigerators after reaction and save.
5) rabbit-anti fibroin albumen antibody is made to the antibody fixer of 0.5mg/ml of protein spots sample liquid, uses protein chip
The spotting needle of point sample instrument carries out point sample on PSG, and point sample terminates to be placed in chip fixed bin, fixes 18.5h at 37 DEG C.
6) after fixed, 35 microlitres of 1%BSA solution is added in every array of substrate, closes 2h under conditions of 37 DEG C.
7) confining liquid is discarded, 5 times (5min/ times) is washed with PBS 7.4 and dries afterwards, takes step 1) treated seven kinds of differences
Each 35 microlitres of supernatant, different arrays is separately added into, using 9.6 buffer of CB as negative control, under conditions of 37 DEG C
It is incubated for 1h.
8) antigen liquid is discarded, 5 times (5min/ times) is washed with PBS 7.4 and dries afterwards, takes step 4) treated quantum dot mark
The goat anti-rabbit antibodies of note are separately added into different arrays, and 35 microlitres of every array is incubated for 1h under conditions of 37 DEG C.
9) secondary antibody liquid is discarded, after being dried afterwards with the washing of PBS 7.4 5 times (5min/ times), uses Fluorescence Scanner Scanning Detction
Fluorescence signal value can detect fluorescence signal if historical relic sample is silk fabrics, on the contrary then do not have.
Embodiment 3
A method of silk fabric cultural relics sample is identified based on protein biochip technology, comprising the following steps:
1) seven kinds of 0.1g different historical relic samples are dissolved in 9.6 buffer of CB of 1000mL, are mixed evenly, it is quiet
It sets
2) by CdCl in 250ml three neck round bottom flask2·2.5H2O(2.5×10-4Mol) it is dissolved in 25ml deionized water
In, glutathione (3.0 × 10 is added-4Mol), two citric acid monohydrate trisodiums (0.1g), Na2TeO3(0.5×10-4Mol) and
NaBH4(2.5×10-4Mol), under stirring, pH to 11 is adjusted with NaOH, be put into the microwave device with reflux, when molten
Liquid color starts the 3h that flows back when being just in light green.
3) dehydrated alcohol is added in the quantum dot selected, and 5min is centrifuged under conditions of 4500rpm, removes supernatant, so
It is repeated 4 times, precipitating is resuspended in PBS 7.4.
4) 25 μ L quantum dot mother liquors (1mg/m1), 20 μ L EDC solutions (10mg/mL) and 10 μ L NHS (1.5mg/ml) are taken
Solution hybrid reaction about 5min.0.2mg goat anti-rabbit antibodies are added, totally 100 μ L marker, reacts 4h under room temperature.Marker
It sets in magnetic stirring apparatus and mixes, make its reaction uniformly, be placed in 4 DEG C of refrigerators after reaction and save.
5) rabbit-anti fibroin albumen antibody is made to the antibody fixer of 0.5mg/ml of protein spots sample liquid, uses protein chip
The spotting needle of point sample instrument carries out point sample on PSG, and point sample terminates to be placed in chip fixed bin, fixes 19h at 37 DEG C.
6) after fixed, 40 microlitres of 1%BSA solution is added in every array of substrate, closes 2h under conditions of 37 DEG C.
7) confining liquid is discarded, 6 times (5min/ times) is washed with PBS 7.4 and dries afterwards, takes step 1) treated three kinds of differences
Each 40 microlitres of supernatant, different arrays is separately added into, using 9.6 buffer of CB as negative control, under conditions of 37 DEG C
It is incubated for 1h.
8) antigen liquid is discarded, 6 times (5min/ times) is washed with PBS 7.4 and dries afterwards, takes step 4) treated quantum dot mark
The goat anti-rabbit antibodies of note are separately added into different arrays, and 40 microlitres of every array is incubated for 1h under conditions of 37 DEG C.
9) secondary antibody liquid is discarded, after being dried afterwards with the washing of PBS 7.4 6 times (5min/ times), uses Fluorescence Scanner Scanning Detction
Fluorescence signal value can detect fluorescence signal if historical relic sample is silk fabrics, on the contrary then do not have.
Raw material used in the present invention, equipment are unless otherwise noted the common raw material of this field, equipment；The present invention
Middle method therefor is unless otherwise noted the conventional method of this field.
Raw materials used in the present invention, equipment is unless otherwise noted the common raw material, equipment of this field；In the present invention
Method therefor is unless otherwise noted the conventional method of this field.
The above is only presently preferred embodiments of the present invention, is not intended to limit the invention in any way, it is all according to the present invention
Technical spirit any simple modification, change and equivalent transformation to the above embodiments, still fall within the technology of the present invention side
The protection scope of case.
Claims (4)
1. a kind of method for identifying silk fabric cultural relics sample based on protein biochip technology, it is characterised in that the following steps are included:
1) the historical relic sample of 0.01g-0.1g is dissolved in 9.6 buffer of CB of 100-1000mL, is mixed evenly, stood,
Taking supernatant is antigen liquid；
2) by CdCl2·2.5H2O is dissolved in deionized water, and glutathione, two citric acid monohydrate trisodiums, Na is added2TeO3With
NaBH4, under stirring, pH is adjusted with NaOH, acquired solution is transferred in the microwave device with reflux, works as solution colour
Start the 2-3h that flows back when in light green, obtains quantum dot；
3) quantum dot is added to absolute ethanol, 3-7min is centrifuged at 3500-4500rpm, remove supernatant, so repeatedly 2-
4 times, precipitating is resuspended in PBS7.4；
4) quantum dot mother liquor obtained by 20-30 μ L step 3), 18-22 μ L EDC solution and 8-12 μ L NHS solution hybrid reaction 3- are taken
7min；0.15-0.25mg goat anti-rabbit antibodies are added, 2-4h is reacted under room temperature, are obtained after reaction quantum dot-labeled
Goat anti-rabbit antibodies are secondary antibody liquid, are placed at 1-5 DEG C and save backup；
5) rabbit-anti fibroin albumen antibody is made to the antibody fixer of 0.4-0.6mg/mL of protein spots sample liquid, uses protein chip
The spotting needle of point sample instrument carries out point sample on PSG, and point sample terminates to be placed in chip fixed bin, fixes 18- at 35-39 DEG C
19h；
6) after fixed, 30-40 microlitres of 0.8-1.2wt%BSA solution is added into the array of gained substrate, as closing
Liquid closes 1.5-2.5h at 35-39 DEG C；
7) confining liquid is discarded, drying after being washed 4-6 times with PBS 7.4 takes obtained by step 1) 30-40 microlitres of antigen liquid, column are added
Battle array, using 9.6 buffer of CB as negative control, is incubated for 0.5-1.5h under conditions of 35-39 DEG C；
8) antigen liquid is discarded, drying after being washed 4-6 times with PBS 7.4 takes secondary antibody liquid obtained by 30-40 microlitres of step 4) that column are added
Battle array, is incubated for 0.5-1.5h under conditions of 35-39 DEG C；
9) secondary antibody liquid is discarded, after being dried after being washed 4-6 time with PBS 7.4, using Fluorescence Scanner Scanning Detction fluorescence signal value,
If historical relic sample is silk fabrics, fluorescence signal, on the contrary then nothing can be detected.
2. a kind of method for being identified silk fabric cultural relics sample based on protein biochip technology as described in claim 1, feature are existed
In, in step 2), the preparation method of the quantum dot specifically: by 2.5 × 10-4The CdCl of mol2·2.5H2O is dissolved in 25mL and goes
In ionized water, it is added 3.0 × 10-4Mol glutathione, 0.1g two citric acid monohydrate trisodiums, 0.5 × 10-4molNa2TeO3With 2.5
×10-4molNaBH4, under stirring, pH to 10-11 is adjusted with NaOH, is put into the microwave device with reflux, works as solution
Color starts the 2-3h that flows back when being just in light green.
3. a kind of method for being identified silk fabric cultural relics sample based on protein biochip technology as described in claim 1, feature are existed
In in step 4), the concentration of the quantum dot mother liquor is 0.8-1.2mg/mL, and EDC solution concentration is 8-12mg/mL, NHS solution
Concentration is 1.3-1.7mg/mL.
4. a kind of method for being identified silk fabric cultural relics sample based on protein biochip technology as described in claim 1, feature are existed
In, step 7), 8) and 9) in, being washed with PBS7.4 is 4-6min/ times.
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