CN110333311B - Automatic extraction and purification device - Google Patents
Automatic extraction and purification device Download PDFInfo
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- CN110333311B CN110333311B CN201910612003.3A CN201910612003A CN110333311B CN 110333311 B CN110333311 B CN 110333311B CN 201910612003 A CN201910612003 A CN 201910612003A CN 110333311 B CN110333311 B CN 110333311B
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- 238000000746 purification Methods 0.000 title claims abstract description 28
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/04—Solvent extraction of solutions which are liquid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
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- Chemical & Material Sciences (AREA)
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Abstract
The invention relates to an automatic extraction and purification device which comprises at least one extraction module, at least three station modules and a first guide rail, wherein the extraction module can move on the first guide rail, the station modules are arranged below the extraction module and comprise at least one zero station module and at least two extraction station modules, and the station modules are sequentially arranged and fixed along the movement direction of the first guide rail. The extraction module comprises an extraction container, a liquid driving device and a second guide rail, wherein the extraction container is connected with the liquid driving device and can move up and down on the second guide rail.
Description
Technical Field
The invention belongs to the field of in-vitro clinical detection sample treatment, and particularly relates to an automatic extraction and purification device.
Background
Mass spectrometry detection is rapidly developed and technically mature in the fields of food safety, environmental protection, chemical pharmacy, forensic criminal investigation and the like, and plays an increasingly important role. The mass spectrum as a high-precision analysis instrument has very strong advantages in the aspects of excellent sensitivity, universality, analysis speed, simultaneous detection of multiple indexes and the like after entering the fields of clinical examination and precise medical treatment, and relates to the application fields of clinical biochemistry, clinical immunity examination, clinical microorganism examination, proteomics, metabonomics scientific research and the like. However, the clinical application of mass spectrometry, particularly the clinical application of LC-MS still has the following problems to be solved:
1. the sample pretreatment steps are various, the process is complex, and the requirement on the skill of a person is high;
2. the method is non-intelligent, has large human interference factors, and is easy to cause unstable results, large errors and high error rate;
3. the experimental period is long, the cost is high, the report period is long, and the clinical requirements are difficult to meet;
4. the degree of informatization is low, and sample information and the experimental process can not be accurately recorded, so that the system in the hospital is difficult to use and is difficult to trace to the source.
The reasons for these problems are mainly that the purification treatment method and the implementation means of the existing sample before entering the mass spectrum are complex, the sample treatment process takes up more than 70% of the time of the whole analysis process, the development of the clinical mass spectrum in the in vitro detection industry is limited, the traditional treatment method is complex to operate, and the purification effect is not parallel due to the sample viscosity, the impurity concentration and the like, so that the whole analysis result is influenced. Therefore, the simple, convenient, accurate and clinical biological sample pretreatment method is a key factor for guaranteeing the accuracy of the measurement result, the reasonability of the measurement index and improving the popularity of the method, can provide a reliable analysis platform for daily detection of clinical biological samples and research of pharmacodynamic relationship of medicines, better ensures the safe and effective medication of clinical patients and improves the treatment effect.
Disclosure of Invention
In order to achieve the above object, the present invention provides an automatic extraction and purification apparatus, which includes at least one extraction module, at least three station modules and a first guide rail, wherein the extraction module can move on the first guide rail, the station modules are disposed below the extraction module, and include at least one zero station module and at least two extraction station modules, and the station modules are sequentially arranged and fixed along the moving direction of the first guide rail. The extraction module comprises an extraction container, a liquid driving device and a second guide rail, wherein the extraction container is connected with the liquid driving device and can move up and down on the second guide rail.
The extraction module comprises an extraction container, a liquid driving device and a second guide rail, the second guide rail is connected with and can drive the extraction container to move up and down, extraction materials are arranged in the extraction container, an upper opening and a lower opening are respectively arranged at the top end and the bottom end of the extraction container, and the upper opening of the extraction container is tightly connected with the liquid driving device.
The extraction container is an extraction component of the automatic extraction and purification device, the extraction container is a cylindrical container, and an extraction material is arranged in the extraction container. For example, the extraction vessel may be a column tube commonly used in solid phase extraction columns, but it will be understood by those skilled in the art that any material of column vessel may be used as long as it can contain the extraction material. Preferably, the extraction vessel is a conical vessel with a large top and a small bottom, and the conical vessel has two advantages: (1) the reagent is convenient to suck from the bottom of the extraction container; (2) the sample solution with small volume can be conveniently extracted, and the error is reduced. More preferably, the extraction vessel is a pipette tip.
The extraction material is selected from granular solid, powdery solid or a porous sieve plate containing extraction filler, and preferably, the extraction material is the sieve plate containing the extraction filler, so that the extraction material cannot move along with the reagent in the extraction container under the action of the liquid driving device, and the extraction material is prevented from being sucked into the liquid driving device.
Preferably, the pore diameter of the sieve plate is 5-100 μm, more preferably, the pore diameter of the sieve plate is 5-20 μm, and the pore diameter of the sieve plate can be adjusted according to the sample to be actually extracted.
The sieve plate is made of corrosion-resistant organic or inorganic materials, such as polypropylene, polytetrafluoroethylene, fibers and the like, and can be reasonably selected according to the physicochemical properties of the actual extracted sample.
Preferably, the extraction material is disposed in a lower middle portion of the extraction vessel, and more preferably, the extraction material is disposed in a bottom portion of the extraction vessel.
The liquid driving device comprises a first interface and a second interface, the upper opening of the extraction container is tightly connected with the first interface of the liquid driving device, and the liquid driving device enables the movement direction of the liquid in the extraction container to be from the first interface to the second interface or from the second interface to the first interface by providing pressure.
Preferably, the liquid driving device is a syringe pump.
Preferably, the liquid driving device provides negative pressure to the extraction container to make the extraction container suck the reagent from the lower opening, and then provides positive pressure to the extraction container to make the liquid in the extraction container discharge from the lower opening.
Preferably, the upper opening of the extraction container is directly and tightly connected with the first interface, at this time, the liquid driving device is arranged above the extraction container, and the second guide rail is directly connected with the liquid driving device and drives the liquid driving device and the extraction container to move up and down together.
Preferably, the upper opening of the extraction container is tightly connected with the first interface through a liquid pipeline, at the moment, the liquid driving device is arranged outside the extraction module, and the second guide rail is connected with the liquid pipeline or a component for supporting the liquid pipeline, so that the second guide rail is indirectly connected with the extraction container, and the second guide rail can drive the extraction container to move up and down.
In an embodiment of the present invention, the liquid driving device is disposed above the extraction container, an upper opening of the extraction container is directly and tightly connected to the first interface of the liquid driving device, and the second guide rail is directly connected to the liquid driving device and drives the liquid driving device and the extraction container to move up and down together.
Preferably, limit switches are arranged on the upper portion and the lower portion of the second guide rail, the limit switches prompt and control the upper limit position and the lower limit position of the movement of the extraction container, and the extraction container and/or the liquid driving device are prevented from exceeding the normal movement range and damaging the station module below the extraction module or the first guide rail above the extraction module.
Preferably, be equipped with liquid level detection device on the extraction module, liquid level detection device includes pressure sensor and capacitive sensor, liquid level detection device detects the inside liquid level of extraction container guarantees that the extraction container can normally absorb reagent in each station module department, avoids the extraction container because of liquid drive arrangement's not enough pressure can't inhale reagent, or liquid drive arrangement's too big liquid entering liquid drive arrangement in with the extraction container of pressure, perhaps the position of extraction module and station module appears the mistake, and makes the extraction container can't inhale reagent. More preferably, the liquid level detection device is disposed at a middle or lower portion of the second guide rail.
Preferably, the number of the extraction modules is 2-100, so as to meet different requirements of small-scale, medium-scale and large-scale detection analysis. For small or medium scale detection assays, the number of extraction modules is preferably 3-6, i.e. the general process of substance extraction, i.e. the steps of activation, extraction, rinsing and elution, etc.
The first guide rail is arranged above the extraction modules, preferably, the top end of the second guide rail is detachably fixed on the first guide rail, and when the automatic extraction and purification device comprises a plurality of extraction modules, the extraction modules are sequentially arranged on the first guide rail.
The shape of the first guide rail is selected from a strip shape or an annular shape, preferably, the first guide rail is an annular guide rail, and the plurality of extraction modules do circular motion on the annular guide rail, so that continuous extraction operation is realized conveniently.
The station modules are arranged below the extraction modules and comprise at least one zero station module and at least two extraction station modules, the zero station modules and the extraction station modules are sequentially arranged and fixed along the movement direction of the first guide rail, namely, the positions of the station modules can vertically correspond to the positions of the extraction modules.
The station module comprises a rotary disc, a transmission mechanism and a storage disc, wherein the hollow bottom of the storage disc and one side of the hollow bottom of the storage disc are provided with openings, the transmission mechanism is arranged below the storage disc, the edge of the rotary disc is provided with at least one sunken clamping position, and the openings of the storage disc correspond to the clamping positions of the rotary disc.
The station module still includes driving motor, driving motor drive the carousel rotates on the horizontal plane, the screens has the opening in the position at carousel edge for joint reagent bottle or extraction container base.
The transmission mechanism is arranged below the storage disc and supports a reagent bottle or an extraction container base inside the storage disc.
The reagent bottle or the extraction container base are filled in the storage disc, the reagent bottle or the extraction container base is supported by the transmission mechanism and moves towards the opening of the storage disc under the driving of the transmission mechanism, the opening of the storage disc only allows one reagent bottle or one extraction container base to pass through, the previous reagent bottle or the previous extraction container base is pushed by the next reagent bottle or the next extraction container base to move out of the opening of the storage disc and enter the clamping position of the rotary disc, and after the clamping position receives one reagent bottle or one extraction container base, the clamping position rotates to a proper position along with the rotary disc under the driving of the driving motor to wait for butt joint of the extraction module.
Preferably, the transmission mechanism is a conveyor belt.
Preferably, the driving motor is arranged below the rotary table and drives the rotary table to rotate on a horizontal plane, and meanwhile, the rotary table drives the clamping position to rotate to different positions.
Preferably, the transmission mechanism and the storage disc are arranged beside the rotating disc, and the opening of the storage disc corresponds to or is in butt joint with the opening of the clamping position of the rotating disc.
Preferably, the storage disc is a frame body only provided with side frames, namely the top surface and the bottom surface of the storage disc are hollow, when the transmission mechanism operates, the position of the storage disc is fixed, and only the reagent bottles or the extraction container bases in the storage disc move along with the transmission mechanism.
Preferably, the station module still includes the discarded object box, the discarded object box is established the below of carousel, more preferably, the discarded object box is established the screens of carousel correspond the below of position, are convenient for accept the reagent bottle or the extraction container base that fall from the screens.
Preferably, a device for pushing the base of the discarded reagent bottle or the extraction container is arranged at the clamping position of the rotating disc.
During the use, extraction container base is placed to the storage dish inside of zero station module, every one is placed to the top of extraction container base extraction container, extraction container's the upper shed that the position of placing is extraction container is upwards, the under shed is downwards.
During the use, the reagent bottle is placed to the storage tray of extraction station module inside, according to every the extraction step difference that extraction station module undertakes, and the reagent in the reagent bottle of every extraction station module is different, for example, all hold the activation liquid in the reagent bottle of the extraction station module that undertakes the activation, all hold sample solution in the reagent bottle of the extraction station module that undertakes adsorption, all hold the eluant in the reagent bottle of the extraction station module that undertakes elution.
Preferably, the number of the zero station modules is 1-5, and the total number of the extraction container bases in each zero station module is not less than the number of samples.
Preferably, the extraction station modules are arranged in groups, and each group of extraction station modules performs a general process of extraction of a sample to be tested, i.e., the steps of activation, extraction, rinsing, elution, and the like, so that preferably, the number of extraction station modules included in a group is 2 to 5.
More preferably, the number of the groups of the extraction station modules is 1-10, so that different requirements of small-scale, medium-scale and large-scale detection and analysis are met. For small scale detection analysis, the number of sets of extraction station modules is preferably 1-3 sets.
The treatment of the extraction vessel after the extraction module of the present invention is used can be done in two ways: (1) manually taking down the used extraction container and discarding; (2) and a movable clamping plate is arranged at a position connected with the upper opening of the extraction container, and the movable clamping plate is propped against the upper opening of the extraction container when moving downwards, so that the extraction container is ejected out of the extraction module and falls into a waste box below the extraction module.
Automatic extraction and purifier still includes controlling means, controlling means includes main control circuit and control button, main control circuit passes through circuit connection and control first guide rail, second guide rail, limit switch, liquid drive device, drive mechanism and driving motor, preferred, main control circuit still passes through circuit connection and control the movable cardboard of the position that extraction container upper shed connects with the device of reagent bottle or extraction container base is abandoned in the promotion of the screens department of carousel. The control key is connected with the main control circuit, so that an operator can operate the control device conveniently.
Preferably, the control device can also have a self-checking function and a sample introduction function, wherein the self-checking function is to perform self-checking on whether all the extraction modules complete all the required steps of the extraction step or not and whether all the components of the automatic extraction and purification device normally operate or not. The reagent bottle of advance kind function for accomplishing the elution passes through sampling device, sends into relevant detecting instrument, carries out follow-up detection, sampling device chooses for use the supporting sampling device of market according to the kind and the model of actual detecting instrument.
The invention also provides a using method of the automatic extraction and purification device, which comprises the following steps:
(1) the extraction module S1 moves to the position of the zero station module through the first guide rail to take the extraction container;
(2) the extraction module S1 moves to the extraction station module G1 through the first guide rail to complete the first step of the extraction step, and meanwhile, the extraction module S2 moves to the position of the zero station module to take the extraction container;
(3) the extraction module S1 moves to the extraction station module G2 through the first guide rail to complete the second step of the extraction step, meanwhile, the extraction module S2 moves to the extraction station module G1 to complete the first action of the extraction step, and meanwhile, the extraction module S3 moves to the position of the zero station module to take the extraction container;
(4) the extraction module S1 moves to the extraction station module G3 through the first guide rail to complete the third action of the extraction step, meanwhile, the extraction module S2 moves to the extraction station module G2 to complete the second action of the extraction step, meanwhile, the extraction module S3 moves to the extraction station module G1 to complete the first action of the extraction step, and meanwhile, the extraction module S4 reaches the position of the zero station module to take an extraction container;
and the steps are analogized, so that all the extraction modules sequentially pass through all the station modules, and each extraction module finishes the extraction step.
For example, in the above method, the active solution is all in the reagent bottles of extraction station module G1, the sample solution is all in the reagent bottles of extraction station module G2, and the eluent is all in the reagent bottles of extraction station module G3. The activating solution, the eluent and the eluent are selected according to the physicochemical properties of the actual detection sample.
The extraction step in the invention comprises the steps of activation, sample adsorption, elution and elution, wherein the steps of sample adsorption and elution are necessary steps, and the steps of activation and elution are optional steps.
The invention also provides application of the automatic extraction and purification device in mass spectrometry, chromatography or liquid chromatography-mass spectrometry detection, wherein the application is application of the automatic extraction and purification device in sample pretreatment.
The automatic extraction and purification device provided by the invention realizes the functions of one-button full-automatic pretreatment, online purification, automatic sample introduction, information tracing and intelligent self-checking of various clinical samples, can effectively solve the long-standing problems and bottlenecks of mass spectrometry, chromatography and particularly liquid chromatography-mass spectrometry detection in the field of clinical detection, and promotes the real wide application of advanced detection technology in the field of clinical detection.
Drawings
FIG. 1 is a view showing the structure of an extraction module
FIG. 2 is a block diagram of an alternative extraction module
FIG. 3 is a block diagram of a workstation module
FIG. 4 is a view showing an overall configuration of an automatic extraction and purification apparatus
FIG. 5 is a side view of the structure of the automatic extraction and purification apparatus
In the attached drawing, 1-an extraction module, 2-an extraction container, 201-a sieve plate, 202-an upper opening, 203-a lower opening, 3-a syringe pump, 4-a second guide rail, 5-a limit switch, 6-a first guide rail, 7-a liquid level detection device, 8-a zero station module, 9-an extraction station module, 10-a rotary table, 1001-a clamping position, 11-a driving motor, 12-a transmission mechanism, 13-a storage disc, 1301-a reagent bottle, 1302-an extraction container base, 14-a waste box and 15-a platform.
Detailed Description
Example 1 extraction Module
The structure diagram of the extraction module of this embodiment is shown in fig. 1, the liquid driving device in this embodiment is a syringe pump 3, the extraction module 1 includes an extraction container 2, the syringe pump 3 and a second guide rail 4, the middle-lower part of the extraction container 2 is provided with a porous sieve plate 201 containing extraction filler, and the top end and the bottom end are respectively provided with an upper opening 202 and a lower opening 203. The injection pump 3 is arranged above the extraction container 2, and the upper opening 202 of the extraction container 2 is tightly connected with the outlet of the injection pump 3. The second guide rail 4 is connected with the injection pump 3 and can drive the injection pump 3 and the extraction container 2 to move up and down together.
The syringe pump 3 supplies a negative pressure to the extraction container 2 to suck the reagent into the extraction container 2 through the lower opening 203, and then the syringe pump 3 supplies a positive pressure to the extraction container 2 to discharge the liquid in the extraction container 2 through the lower opening 203.
The upper portion and the lower part of second guide rail 4 are equipped with limit switch 5, and limit switch 5 suggestion and control extraction container 2 and the upper limit and the lower limit position of syringe pump 3 motion prevent that extraction container 2 and syringe pump 3 from exceeding normal moving range, destroying the station module of extraction module 1 below, or the first guide rail 6 of extraction module 1 top.
The lower part of second guide rail 4 is equipped with liquid level detection device 7, liquid level detection device 7 includes pressure sensor and capacitive sensor, liquid level detection device 7 detects the inside liquid level of extraction container 2, guarantee that extraction container 2 can normally absorb reagent in each station module department, avoid extraction container 2 because of the not enough unable reagent of suction of the pressure of syringe pump 3, or syringe pump 3's too big liquid entering syringe pump 3 that will extract in the container 2, perhaps the position mistake appears in extraction module 1 and the station module, and make extraction container 2 can't inhale reagent.
EXAMPLE 2 extraction Module
The structure diagram of the extraction module of this embodiment is shown in fig. 2, the extraction module 1 comprises an extraction container 2, a syringe pump 3 and a second guide rail 4, the middle lower part of the extraction container 2 is provided with a porous sieve plate 201 containing extraction packing, and the top end and the bottom end are respectively provided with an upper opening 202 and a lower opening 203. The upper opening 202 of the extraction container 2 is closely connected with the outlet of the injection pump 3 through a liquid pipeline, the injection pump 3 is arranged outside the extraction module 1, and the second guide rail 4 is connected with a component for supporting the liquid pipeline, so that the extraction container 2 is indirectly connected, and the second guide rail 4 can drive the extraction container 2 to move up and down.
The other structure of the extraction module 1 of the present embodiment is the same as that of the extraction module 1 of embodiment 1.
EXAMPLE 3 station Module
The station module structure of this embodiment is shown in fig. 3, and the station modules include a zero station module 8 and an extraction station module 9. The station module comprises a rotary table 10, a driving motor 11, a transmission mechanism 12 and a storage disc 13.
The driving motor 11 is arranged below the turntable 10, the driving motor 11 drives the turntable 10 to rotate on a horizontal plane, a concave clamping position 1001 is arranged at the edge of the turntable 10, an opening is formed in the position of the edge of the turntable 10 of the clamping position 1001 and used for clamping the reagent bottle 1301 or the extraction container base 1302, and the turntable 10 drives the clamping position 1001 to rotate to different positions.
The transmission mechanism 12 and the storage disc 13 are arranged beside the rotary disc 10, the transmission mechanism 12 is arranged below the storage disc 13 and supports the reagent bottle 1301 or the extraction container base 1302 inside the storage disc 13, the bottom of the storage disc 13 is hollow, an opening is formed in one side of the storage disc 13, and the opening of the storage disc 13 corresponds to the clamping position 1001 of the rotary disc 10. The transmission mechanism 12 of this embodiment is a conveyor belt.
The storage tray 13 is a frame body only having side frames, that is, the top surface and the bottom surface of the storage tray 13 are hollow, when the transmission mechanism 12 operates, the position of the storage tray 13 is fixed, and only the reagent bottles 1301 in the storage tray 13 move along with the transmission mechanism 12. The storage tray 13 is filled with reagent bottles 1301, the reagent bottles 1301 are supported by the transmission mechanism 12 and move towards the opening of the storage tray 13 under the driving of the transmission mechanism 12, the opening of the storage tray 13 only allows one reagent bottle 1301 to pass through, the previous reagent bottle 1301 is pushed by the next reagent bottle 1301 to move out of the opening of the storage tray 13 and enter the blocking position 1001 of the rotary disc 10, after the blocking position 1001 receives one reagent bottle 1301, the reagent bottle 1301 rotates 180 degrees along with the rotary disc 10 under the driving of the driving motor 11, the extraction module 1 is in butt joint, then the reagent bottle 1301 rotates 90 degrees, and falls into the waste box 14.
In use, the storage tray 13 of the zero station module 8 is filled with extraction container bases 1302, one extraction container 2 is placed on the top of each extraction container base 1302, and the extraction containers 2 are placed in positions that the upper opening 202 of the extraction container 2 faces upward and the lower opening 203 faces downward.
During the use, the storage tray 13 of extraction station module 9 is inside to be filled with reagent bottle 1301, according to the difference of the extraction step that each extraction station module undertakes, the reagent in the reagent bottle of each extraction station module 9 is different, all hold the activation liquid in the reagent bottle of the extraction station module that undertakes the activation, all hold sample solution in the reagent bottle of the extraction station module that undertakes adsorption, all hold the leacheate in the reagent bottle of the extraction station module that undertakes the leacheate, all hold the eluant in the reagent bottle of the extraction station module that undertakes the elution.
EXAMPLE 4 automatic extraction and purification apparatus
The extraction module of this embodiment is the extraction module of embodiment 1, the extraction station module is the extraction station module of embodiment 3, and the zero station module is the zero station module of embodiment 3.
The structure of the automatic extracting and purifying apparatus of the present embodiment is shown in fig. 4 and 5, the first guide rail 6 is disposed above the extracting module 1, the first guide rail 6 is a circular guide rail, and the top end of the second guide rail 4 is detachably fixed on the lower surface of the first guide rail 6. The automatic extraction and purification device comprises six extraction modules 1 which are sequentially arranged on a first guide rail 6, and the six extraction modules 1 move circularly on the first guide rail 6, so that continuous extraction operation is realized conveniently.
The station module is fixed on the platform 15 below the extraction module 1, the station module comprises a zero station module 8 and an extraction station module 9, the zero station module 8 and the extraction station module 9 are sequentially arranged and fixed along the movement direction of the first guide rail 6, the position of the station module can vertically correspond to the position of the extraction module 1, namely, the extraction container 2 of the extraction module 1 corresponds to the position of the clamping position 1001 of the station module turntable 10, and the extraction module 1 is convenient to extract the extraction container 2 or absorb reagents from the reagent bottle 1301.
The station modules comprise a zero station module 8 and two groups of extraction station modules 9, and each group of extraction station modules 9 comprises three extraction station modules 9. The number of extraction container bases 1302 in the zero-station module 8 is 38, and the number of reagent bottles 1301 in the extraction station module 9 is 38.
The waste 14 cassette is disposed below the position corresponding to the position of the detent 1001 of each station module carousel 10, and is adapted to receive a reagent bottle 1301 or an extraction container base 1302 falling from the detent 1001.
And a movable clamping plate is arranged at the position connected with the upper opening of the extraction container 2, and the movable clamping plate is propped against the upper opening of the extraction container 2 when moving downwards, so that the extraction container 2 is ejected out of the extraction module 1 and falls into the waste box 14 below. The pushing device for pushing the discarded reagent bottle 1301 or the extraction container base 1302 is disposed on the lower surface of the first guide rail 6 and corresponds to the position of the position-limiting stop 1001, and is a push rod capable of moving up and down, and the push rod moves down to abut against and push the reagent bottle 1301 or the extraction container base 1302 in the position-limiting stop 1001 down, so as to push the reagent bottle 1301 or the extraction container base 1302 out of the position-limiting stop 1001 and drop the reagent bottle or the extraction container base 1302 into the waste 14 below the turntable 10.
The automatic extraction and purification device further comprises a control device, the control device comprises a main control circuit and a control key, the main control circuit is connected with and controls the first guide rail 6, the second guide rail 4, the limit switch 5, the injection pump 3, the transmission mechanism 12, the driving motor 11, the movable clamping plate at the position connected with the upper opening of the extraction container 2 and a device for pushing the discarded reagent bottle 1301 or the extraction container base 1302 through circuits, so that all parts can operate in the mode of the embodiments 1, 3 and 4. The control key is connected with the main control circuit, so that an operator can conveniently start or finish extraction operation.
Example 5 method of use
The automatic extraction and purification apparatus of this embodiment includes one zero station module, five extraction station modules (G1-G5), and five extraction modules (S1-S5), as shown in table 1, and the other structures are the same as those of the automatic extraction and purification apparatus of embodiment 4.
TABLE 1 station module configuration
| Zero station 0 | Station G1 | Station G2 | Station G3 | Station G4 | Station G5 | |
| Extraction | Activating solution | 1 | Activating |
Sample (I) | |
|
The method of use of the automatic extraction and purification apparatus of this example is as follows:
(1) the extraction module S1 moves to the position of the zero station module 8 through the first guide rail 6, and the extraction container 2 is taken;
(2) the extraction module S1 moves to the extraction station module G1 through the first guide rail 6 to complete the first activation, and meanwhile, the extraction module S2 moves to the position of the zero station module 8 to take the extraction container 2;
(3) the extraction module S1 moves to the extraction station module G2 through the first guide rail 6 to complete the second activation, meanwhile, the extraction module S2 moves to the extraction station module G1 to complete the first activation, and meanwhile, the extraction module S3 moves to the position of the zero station module 8 to take the extraction container 2;
(4) the extraction module S1 moves to the extraction station module G3 through the first guide rail 6 to complete sample adsorption, meanwhile, the extraction module S2 moves to the extraction station module G2 to complete second activation, meanwhile, the extraction module S3 moves to the extraction station module G1 to complete first activation, and meanwhile, the extraction module S4 reaches the position of the zero station module 8 to take the extraction container 2;
(5) the extraction module S1 moves to the extraction station module G4 through the first guide rail 6 to complete the leaching step, meanwhile, the extraction module S2 reaches the extraction station module G3 to complete the sample adsorption, meanwhile, the extraction module S3 reaches the extraction station module G2 to complete the second activation, meanwhile, the extraction module S4 reaches the extraction station module G1 to complete the first activation, and meanwhile, the extraction module S5 reaches the position of the zero station module 8 to take the extraction container 2;
(6) the extraction module S1 moves to the extraction station module G5 through the first guide rail 6 to complete an elution step, the eluted reagent bottle 1301 is taken out to be detected, meanwhile, the extraction module S2 reaches the extraction station module G4 to complete a leaching step, meanwhile, the extraction module S3 reaches the extraction station module G3 to complete sample adsorption, meanwhile, the extraction module S4 reaches the extraction station module G2 to complete second activation, and meanwhile, the extraction module S5 reaches the extraction station module G1 to complete first activation;
(7) the extraction module S1 moves to the position of the zero station module 8 again through the first guide rail 6, the extraction container 2 is taken, meanwhile, the extraction module S2 reaches the position of the extraction station module G5, the elution step is completed, the eluted reagent bottle is taken out to be detected, meanwhile, the extraction module S3 reaches the position of the extraction station module G4, the leaching step is completed, meanwhile, the extraction module S4 reaches the position of the extraction station module G3, the sample adsorption is completed, meanwhile, the extraction module S5 reaches the position of the extraction station module G2, and the second activation is completed;
and so on, the above steps are circulated until all the sample extraction at the extraction station module G3 is completed.
Claims (4)
1. An automatic extraction and purification device is characterized by comprising at least one extraction module, at least three station modules, a first guide rail and a control device, wherein the extraction module can move on the first guide rail, the station modules are arranged below the extraction module and comprise at least one zero station module and at least two extraction station modules, and the station modules are sequentially arranged and fixed along the movement direction of the first guide rail;
the extraction module comprises an extraction container, a liquid driving device and a second guide rail, wherein the extraction container is a cylindrical container, extraction materials are arranged in the extraction container, an upper opening and a lower opening are respectively formed in the top end and the bottom end of the extraction container, and the upper opening of the extraction container is tightly connected with the liquid driving device and can move up and down on the second guide rail;
the liquid driving device comprises a first interface and a second interface, the upper opening of the extraction container is tightly connected with the first interface of the liquid driving device, and the liquid driving device enables the movement direction of the liquid in the extraction container to be from the first interface to the second interface or from the second interface to the first interface by providing pressure;
the station module comprises a rotary table, a transmission mechanism, a storage disc and a driving motor;
the bottom of the storage disc is hollow, an opening is formed in one side of the storage disc, the transmission mechanism is arranged below the storage disc, at least one sunken clamping position is arranged on the edge of the rotary disc, the opening of the storage disc corresponds to the clamping position of the rotary disc, the drive motor drives the rotary disc to rotate on the horizontal plane, and the clamping position is provided with an opening at the edge of the rotary disc and used for clamping a reagent bottle or an extraction container base;
an extraction container base is placed in the storage disc of the zero station module, and an extraction container base is placed in the storage disc of the extraction station module;
the control device comprises a main control circuit and a control key, the main control circuit is connected with and controls the first guide rail, the second guide rail, the liquid driving device, the transmission mechanism and the driving motor through circuits, and the control key is connected with the main control circuit.
2. The automatic extraction and purification device of claim 1, wherein a liquid level detection device is disposed on the extraction module, and the liquid level detection device comprises a pressure sensor and a capacitance sensor.
3. The automatic extraction and purification apparatus of claim 1, wherein the first guide rail is disposed above the extraction module, and the top end of the second guide rail is detachably fixed to the first guide rail.
4. Use of the automatic extraction and purification device of claim 1 in mass spectrometry, chromatography or combined liquid chromatography and mass spectrometry detection, wherein the use is of the automatic extraction and purification device in sample pretreatment.
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| WO2013008502A1 (en) * | 2011-07-08 | 2013-01-17 | 株式会社日立ハイテクノロジーズ | Solid phase extraction device and viscosity measurement device |
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| CN107085118A (en) * | 2010-10-29 | 2017-08-22 | 恩姆菲舍尔科技公司 | Automated systems and methods for sample preparation and analysis |
| CN108697948A (en) * | 2015-12-29 | 2018-10-23 | 生命科技股份有限公司 | continuous sample purification system and method |
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