CN110331083A - A kind of digital pcr chip and its operating method - Google Patents
A kind of digital pcr chip and its operating method Download PDFInfo
- Publication number
- CN110331083A CN110331083A CN201910599865.7A CN201910599865A CN110331083A CN 110331083 A CN110331083 A CN 110331083A CN 201910599865 A CN201910599865 A CN 201910599865A CN 110331083 A CN110331083 A CN 110331083A
- Authority
- CN
- China
- Prior art keywords
- chip
- layers
- droplet
- fixedly connected
- pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
The invention belongs to technical field of molecular biology, in particular a kind of digital pcr chip, item is placed including chip, the inner sidewall that the chip places item is fixedly connected with B layers of chip base, the inner sidewall of the B layers of chip base is fixedly connected with droplet chip, and the upper surface of the droplet chip is fixedly connected with droplet chip constant volume ring.The present invention merges into a single whole PCR droplet forming process and PCR amplification process progress, so that entire detection system more minimizes and integration, improve working efficiency, by the way that pressure push-rod is arranged, pressure conduction aluminium film and pressure conduction film, since the material of pressure sensing membrane is tetrafluoride material, tension has certain limitations, therefore it is constant pressure that pressure conduction film, which deforms generated secondary pressure, it will not rack or break up in the environment of transfiniting pressure by the sample of oil package, droplet sample is avoided to form pie, it is set to pass through chip droplet hole, play limit transmission, precisely enter the effect of drip hole.
Description
Technical field
The present invention relates to technical field of molecular biology, specially a kind of digital pcr chip and its operating method.
Background technique
Polymerase chain reaction is a kind of for amplifying the Protocols in Molecular Biology for expanding specific DNA fragmentation, it can see
Work is the special DNA replication dna of in vitro.It has been widely applied to the molecular biosciences such as genetic test, gene magnification, genetic engineering
Field, and clinical medicine, medical jurisprudence, paternity test and in terms of play irreplaceable role,
However PCR reaction is with exponential amplification, is amplifiable million times in dozens of minutes, it is difficult to determine original PCR by PCR product
The content of template.In order to which accurate quantification analyzes nucleic acid content, digital technology is invented, the basic principle is that by a sample equivalent
Tens are diluted to tens of thousands of parts, is assigned to different reaction members, each unit includes the target molecule of zero or one copy,
The PCR amplification of single copy DNA is independently carried out in each reaction member, the DNA fragmentation amplified in independent reaction member can
With the fluorescence display issued by sequence-specific probes molecule, after amplification, the reaction member comprising target molecule has glimmering
Optical signal is denoted as the positive, and then fluorescence signal is not denoted as feminine gender to the reaction member of target molecule, by positive ratio is
The copy number of target nucleic acid template in initial sample can be calculated.
Existing digital pcr chip need in operation multiple equipment carry out cooperative cooperating, PCR droplet molding and
PCR amplification is separately operated, and working efficiency is low, and cost is also larger.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, it the present invention provides a kind of digital pcr chip and its operating method, solves multiple
Equipment carries out cooperative cooperating, ineffective problem.
(2) technical solution
To achieve the above object, the invention provides the following technical scheme: a kind of digital pcr chip, including chip place item,
The inner sidewall that the chip places item is fixedly connected with B layers of chip base, and the inner sidewall of the B layers of chip base is fixedly connected with
Droplet chip, the upper surface of the droplet chip are fixedly connected with droplet chip constant volume ring, the upper surface of the B layers of chip base
A layers of chip base are fixedly connected with, the upper surface of the A layers of chip base offers sample hole location and oil transportation hole location, institute respectively
The side for stating A layers of chip base offers pipe interface, and the droplet chip includes micropore, in being internally provided with of the micropore
Between hole, the inner wall of the interstitial hole is fixedly connected with partition, and the upper surface of the A layers of chip base is fixedly connected with pressure conduction
Film, the upper surface of the pressure conduction film are fixedly connected with pressure conduction aluminium film, and the top slide of the pressure conduction aluminium film connects
It is connected to pressure push-rod, the left end that the chip places item is provided with push fixator, and the chip places the fixed company of right end of item
It is connected to PCR heater, the back side of the PCR heater is fixedly connected with A layers of chip base vacuum pump, the PCR heater
Right side is equipped with PCR detector, and the PCR detector is fixedly connected with A layers of chip base vacuum pump, the right side of the PCR detector
Side wall is fixedly connected with waste liquid cup handgrip, and the lower right of the waste liquid cup handgrip is equipped with liquid waste collector, the waste liquid cup handgrip
It is fixedly connected with liquid waste collector, the right end of the liquid waste collector is fixedly connected with B layers of chip base vacuum pump.
As a preferred technical solution of the present invention, the intersection of the sample hole location and oil transportation hole location is provided with mixed liquid
The side in area, the mixed liquid zone is connected to droplet chip, and the side of the pipe interface is connected to droplet chip.
As a preferred technical solution of the present invention, the shape of the micropore is dished circular structure, each described micro-
Hole includes 4-6 piece partition, and the shape of the partition is sector, is provided with gap between the partition.
As a preferred technical solution of the present invention, the shape of the interstitial hole is that circular ring shape is poroid, under the partition
Incline 45 °.
As a preferred technical solution of the present invention, conduit in the A layers of chip base respectively with A layers of chip base
Vacuum pump, B layers of chip base vacuum pump are connected perforation.
As a preferred technical solution of the present invention, the pressure conduction aluminium film and pressure conduction film pass through bonding way
It is fixedly connected.
A kind of operating method of digital pcr chip, operating procedure are as follows:
S1: cleaning is placed, the humidity strip that the placement region of A layers of chip base, B layers of chip base was impregnated by cleaning liquid
Wiping cleaning is carried out, prevents there are other substances, then places the biomass for needing to process on A layers of chip base；
S2: compacting pushes pressure push-rod, acts on and applies a pressure in pressure conduction aluminium film, makes to press
Power conducts aluminium film and generates deformation, thus the air between compression pressure conduction aluminium film and pressure conduction film；
S3: constant volume, compressed raw material object, through the space of droplet chip constant volume ring inner hole under the action of droplet chip
And the thickness of constant volume ring is determined by the amount of the mixing liquid needed for analyzing, so as to ensure to fill up the mixing liquid of constant volume ring
Volume it is precisely errorless；
S4: at liquid, the sample droplets at sample hole location are collected with the oil droplet at oil transportation hole location in mixed liquid zone, and oil droplet is by sample
Drop package, and nanoscale mixing drop is formed at droplet chip；
S5: push, nanoscale mixing drop enters in B layers of chip base from micropore, then B layers of chip base are put into core
Piece is placed in the placing groove in item, places one push pressure of item to chip by push fixator, pipe interface is put with chip
The conveyance conduit connection in item is set, sealing ring when two repeatedly kick into row connection between them can play certain sealing and make
With；
S6: heating detection starts A layer chip base vacuum pump, the B layers of chip base vacuum pump of junction, so that PCR adds
Hot device, PCR detector bring into operation, and compare inspection by normal data volume after the molecule object completed the process is heated
It surveys.
(3) beneficial effect
Compared with prior art, the present invention provides a kind of digital pcr chip and its operating methods, have following beneficial to effect
Fruit:
1, the digital pcr chip and its operating method, PCR droplet forming process and PCR amplification process are merged into a single whole
It carries out, so that entire detection system more minimizes and integration, working efficiency is improved, by the way that pressure push-rod, pressure is arranged
Aluminium film and pressure conduction film are conducted, since the material of pressure sensing membrane is tetrafluoride material, tension is had certain limitations, therefore pressure passes
It is constant pressure that guided membrane, which deforms generated secondary pressure, will not rack or break up in the environment of transfiniting pressure and be wrapped by oil
The sample wrapped up in avoids droplet sample from forming pie, it is made to pass through chip droplet hole, plays limit transmission, precisely enters
The effect of drip hole.
2, the digital pcr chip and its operating method make droplet core by being equipped with droplet chip constant volume ring and droplet chip
The thickness of piece constant volume ring by needed for analyzing the amount of mixing liquid and the etching micropore gross area of droplet chip determine, in this way can be with
Ensure to fill up the mixing liquid in droplet chip constant volume ring volume be precisely it is errorless, effect is made to improve.
Detailed description of the invention
Fig. 1 is flowage structure schematic diagram of the present invention；
Fig. 2 is chip pressing structure schematic diagram of the present invention；
Fig. 3 is pressure conduction membrane structure diagram of the present invention；
Fig. 4 is A layers of chip base of the present invention and B layers of chip base attachment structure schematic diagram one；
Fig. 5 is A layers of chip base of the present invention and B layers of chip base attachment structure schematic diagram two；
Fig. 6 is A layers of chip base of the present invention and B layers of chip base attachment structure schematic diagram three；
Fig. 7 is A layers of chip base structural schematic diagram one of the present invention；
Fig. 8 is A layers of chip base structural schematic diagram two of the present invention；
Fig. 9 is A layers of chip base structural schematic diagram three of the present invention；
Figure 10 is microcellular structure schematic diagram one of the present invention；
Figure 11 is microcellular structure schematic diagram two of the present invention.
In figure: 1, pressure push-rod；2, pressure conduction aluminium film；3, pressure conduction film；4, A layers of chip base；5, sample hole location；
6, oil transportation hole location；7, droplet chip constant volume ring；8, droplet chip；9, B layers of chip base；10, fixator is pushed；11, chip is put
Set item；12, PCR heater；13, PCR detector；14, A layers of chip base vacuum pump；15, waste liquid cup handgrip；16, waste collection
Device；17, B layers of chip base vacuum pump；18, liquid zone is mixed；19, pipe interface；20, micropore；21, interstitial hole；22, partition.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment
Please refer to Fig. 1-5, the present invention the following technical schemes are provided: a kind of digital pcr chip, including chip place item 11,
The inner sidewall that chip places item 11 is fixedly connected with B layers of chip base 9, and the inner sidewall of B layers of chip base 9 is fixedly connected with droplet
Chip 8, the upper surface of droplet chip 8 are fixedly connected with droplet chip constant volume ring 7, and the upper surface of B layers of chip base 9 is fixedly connected
There are A layers of chip base 4, the upper surface of A layers of chip base 4 offers sample hole location 5 and oil transportation hole location 6, A layers of chip base respectively
4 side offers pipe interface 19, and droplet chip 8 includes micropore 20, and micropore 20 is internally provided with interstitial hole 21, interstitial hole
21 inner wall is fixedly connected with partition 22, and the upper surface of A layers of chip base 4 is fixedly connected with pressure conduction film 3, pressure conduction film
3 upper surface is fixedly connected with pressure conduction aluminium film 2, and the top slide of pressure conduction aluminium film 2 is connected with pressure push-rod 1, chip
The left end for placing item 11 is provided with push fixator 10, and the right end that chip places item 11 is fixedly connected with PCR heater 12, PCR
The back side of heater 12 is fixedly connected with A layers of chip base vacuum pump 14, and the right side of PCR heater 12 is equipped with PCR detector 13,
PCR detector 13 is fixedly connected with A layers of chip base vacuum pump 14, and the right side wall of PCR detector 13 is fixedly connected with waste liquid cup
Handgrip 15, the lower right of waste liquid cup handgrip 15 are equipped with liquid waste collector 16, waste liquid cup handgrip 15 and the fixed company of liquid waste collector 16
It connects, the right end of liquid waste collector 16 is fixedly connected with B layers of chip base vacuum pump 17.
In the present embodiment, PCR droplet forming process and PCR amplification process are merged into a single whole progress, so that entire inspection
Examining system more minimizes and integration, improves working efficiency, passes through setting pressure push-rod 1, pressure conduction aluminium film 2 and pressure
Conductive membranes 3, since the material of pressure sensing membrane is tetrafluoride material, tension is had certain limitations, therefore the deformation of pressure conduction film 3 is produced
Raw secondary pressure is constant pressure, will not rack or break up in the environment of transfiniting pressure by the sample of oil package, keep away
Exempt from droplet sample and form pie, it is made to pass through chip droplet hole, plays limit transmission, precisely enter the effect of drip hole.
Specifically, the intersection of the sample hole location 5 and oil transportation hole location 6 is provided with mixed liquid zone 18, the side of liquid zone 18 is mixed
It is connected to droplet chip 8, the side of pipe interface 19 is connected to droplet chip 8.
In the present embodiment, the position oil droplet in mixed liquid zone 18 is mixed with sample droplets, and oil droplet simultaneously wraps up sample droplets.
Specifically, the shape of micropore 20 is dished circular structure, each micropore 20 includes 4-6 piece partition 22, partition 22
Shape is sector, is provided with gap between partition 22.
In the present embodiment, gap between partition 22 slides and sinks convenient for drop, avoids impact force when squeezing
Drop is broken up, prevents oil droplet from separating with sample droplets.
Specifically, the shape of interstitial hole 21 is that circular ring shape is poroid, partition 22 has a down dip 45 °.
In the present embodiment, 45° angle is beneficial to mix gathering and gliding for drop.
Specifically, conduit in A layers of chip base 4 respectively with A layers of chip base vacuum pump 14, B layers of chip base vacuum
Pump 17 is connected perforation.
In the present embodiment, so that A layers of chip base 4 pass through conduit and A layers of chip base vacuum pump 14, B layers of chip base
Vacuum pump 17 is connected, to carry out heating detection operation.
Specifically, pressure conduction aluminium film 2 is fixedly connected with pressure conduction film 3 by bonding way.
In the present embodiment, so that pressure conduction aluminium film 2 is stably connected with pressure conduction film 3, to carry out external pressure
It is attached transmission.
A kind of operating method of digital pcr chip, operating procedure are as follows:
S1: cleaning is placed, and the placement region of A layers of chip base 4, B layers of chip base 9 is impregnated by cleaning liquid wet
Piece carries out wiping cleaning, prevents there are other substances, then the biomass for needing to process is placed on A layers of chip base 4；
S2: compacting pushes pressure push-rod 1, acts on and applies a pressure in pressure conduction aluminium film 2, makes
Pressure conduction aluminium film 2 generates deformation, thus the air between compression pressure conduction aluminium film 2 and pressure conduction film 3；
S3: constant volume, compressed raw material object, by the space of 7 inner hole of droplet chip constant volume ring droplet chip 8 effect
Lower and constant volume ring thickness is determined by the amount of the mixing liquid needed for analyzing, so as to ensure to fill up the mixed liquor of constant volume ring
The volume of body is precisely errorless；
S4: at liquid, the sample droplets at sample hole location 5 are collected with the oil droplet at oil transportation hole location 6 in mixed liquid zone 18, and oil droplet will
Sample droplets package, and nanoscale mixing drop is formed at droplet chip 8；
S5: push, nanoscale mixing drop enters in B layers of chip base 9 from micropore 20, then B layers of chip base 9 are put
Enter in the placing groove that chip is placed in item 11,11 1 push pressure of item is placed to chip by push fixator 10, pipeline connects
Mouth 19 is placed the conveyance conduit in item 11 with chip and is connected to, and sealing ring when two repeatedly kick into row connection between them can rise
To certain sealing function；
S6: heating detection starts A layer chip base vacuum pump 14, the B layers of chip base vacuum pump 17 of junction, thus
PCR heater 12, PCR detector 13 bring into operation, after the molecule object completed the process is heated by normal data volume into
Row contrasting detection.
In conclusion PCR droplet forming process and PCR amplification process are merged into a single whole progress, so that entire detection system
System more miniaturization and integration, improves working efficiency.
It should be noted that, in this document, such as the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in process, method, article or equipment including the element.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of digital pcr chip, including chip place item (11), it is characterised in that: the chip places the inside of item (11)
Wall is fixedly connected with B layers of chip base (9), and the inner sidewall of the B layers of chip base (9) is fixedly connected with droplet chip (8), institute
The upper surface for stating droplet chip (8) is fixedly connected with droplet chip constant volume ring (7), and the upper surface of the B layers of chip base (9) is solid
Surely A layers of chip base (4) are connected with, the upper surface of the A layers of chip base (4) offers sample hole location (5) and oil transportation respectively
The side of hole location (6), the A layers of chip base (4) offers pipe interface (19), and the droplet chip (8) includes micropore
(20), the micropore (20) is internally provided with interstitial hole (21), and the inner wall of the interstitial hole (21) is fixedly connected with partition
(22), the upper surface of the A layers of chip base (4) is fixedly connected with pressure conduction film (3), the pressure conduction film (3) it is upper
Surface is fixedly connected with pressure conduction aluminium film (2), and the top slide of the pressure conduction aluminium film (2) is connected with pressure push-rod (1),
The left end that the chip places item (11) is provided with push fixator (10), and the right end that the chip places item (11) is fixedly connected
Have PCR heater (12), the back side of the PCR heater (12) is fixedly connected with A layers of chip base vacuum pump (14), described
The right side of PCR heater (12) is equipped with PCR detector (13), the PCR detector (13) and A layers of chip base vacuum pump (14)
It is fixedly connected, the right side wall of the PCR detector (13) is fixedly connected with waste liquid cup handgrip (15), the waste liquid cup handgrip (15)
Lower right be equipped with liquid waste collector (16), the waste liquid cup handgrip (15) is fixedly connected with liquid waste collector (16), described to give up
The right end of collection (16) is fixedly connected with B layers of chip base vacuum pump (17).
2. a kind of digital pcr chip according to claim 1, it is characterised in that: the sample hole location (5) and oil transportation hole location
(6) intersection is provided with mixed liquid zone (18), and the side of the mixed liquid zone (18) is connected to droplet chip (8), and the pipeline connects
The side of mouth (19) is connected to droplet chip (8).
3. a kind of digital pcr chip according to claim 1, it is characterised in that: the shape of the micropore (20) is recess
Circular configuration, each micropore (20) includes 4-6 piece partition (22), and the shape of the partition (22) is sector, the partition
(22) gap is provided between.
4. a kind of digital pcr chip according to claim 1, it is characterised in that: the shape of the interstitial hole (21) is circle
Annular is poroid, and the partition (22) has a down dip 45 °.
5. a kind of digital pcr chip according to claim 1, it is characterised in that: leading in the A layers of chip base (4)
Pipe is connected with A layers of chip base vacuum pump (14), B layers of chip base vacuum pump (17) perforation respectively.
6. a kind of digital pcr chip according to claim 1, it is characterised in that: the pressure conduction aluminium film (2) and pressure
Conductive membranes (3) are fixedly connected by bonding way.
7. a kind of operating method of digital pcr chip according to claim 1-6, which is characterized in that operation step
It is rapid as follows:
S1: cleaning is placed, and the placement region of A layers of chip base (4), B layers of chip base (9) is impregnated by cleaning liquid wet
Piece carries out wiping cleaning, prevents there are other substances, then the biomass for needing to process is placed on A layers of chip base (4)；
S2: compacting pushes pressure push-rod (1), acts on and applies a pressure on pressure conduction aluminium film (2), makes
Pressure conduction aluminium film (2) generates deformation, thus the air between compression pressure conduction aluminium film (2) and pressure conduction film (3)；
S3: constant volume, compressed raw material object, the effect by the space of droplet chip constant volume ring (7) inner hole in droplet chip (8)
Lower and constant volume ring thickness is determined by the amount of the mixing liquid needed for analyzing, so as to ensure to fill up the mixed liquor of constant volume ring
The volume of body is precisely errorless；
S4: at liquid, the sample droplets at sample hole location (5) are collected with the oil droplet at oil transportation hole location (6) in mixed liquid zone (18), oil droplet
Sample droplets are wrapped up, and form nanoscale mixing drop at droplet chip (8)；
S5: push, nanoscale mixing drop enters in B layers of chip base (9) from micropore (20), then by B layers of chip base (9)
It is put into the placing groove that chip is placed in item (11), (11) push pressures of item is placed to chip by push fixator (10)
Power, pipe interface (19) are placed the conveyance conduit in item (11) with chip and are connected to, when two repeatedly kick into row connection between them
Sealing ring can play certain sealing function；
S6: heating detection starts the A layer chip base vacuum pump (14) of junction, B layers of chip base vacuum pump (17), thus
PCR heater (12), PCR detector (13) bring into operation, and pass through normal data after the molecule object completed the process is heated
Volume compares detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910599865.7A CN110331083A (en) | 2019-07-04 | 2019-07-04 | A kind of digital pcr chip and its operating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910599865.7A CN110331083A (en) | 2019-07-04 | 2019-07-04 | A kind of digital pcr chip and its operating method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110331083A true CN110331083A (en) | 2019-10-15 |
Family
ID=68143117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910599865.7A Pending CN110331083A (en) | 2019-07-04 | 2019-07-04 | A kind of digital pcr chip and its operating method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110331083A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102016314A (en) * | 2008-08-26 | 2011-04-13 | 松下电器产业株式会社 | Fluid transport using conductive polymer |
| CN103343092A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院上海微系统与信息技术研究所 | Method for manufacturing digital PCR (polymerase chain reaction) chip based on mineral-oil saturated PDMS (polydimethylsiloxane) material |
| CN107532990A (en) * | 2015-05-12 | 2018-01-02 | 芯片生物技术株式会社 | Single particle analytic method and the system for the parsing |
| US20180067038A1 (en) * | 2015-03-19 | 2018-03-08 | The Board Of Trustees Of The Leland Stanford Junior University | Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip |
| CN108251269A (en) * | 2017-12-08 | 2018-07-06 | 深圳市博瑞生物科技有限公司 | Digital pcr droplet generating means |
| CN109415671A (en) * | 2017-04-10 | 2019-03-01 | 古河电气工业株式会社 | Liquid feeding device and feeding method |
| CN109738632A (en) * | 2019-01-09 | 2019-05-10 | 南京岚煜生物科技有限公司 | Multi objective micro-fluidic chip and its application method |
-
2019
- 2019-07-04 CN CN201910599865.7A patent/CN110331083A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102016314A (en) * | 2008-08-26 | 2011-04-13 | 松下电器产业株式会社 | Fluid transport using conductive polymer |
| CN103343092A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院上海微系统与信息技术研究所 | Method for manufacturing digital PCR (polymerase chain reaction) chip based on mineral-oil saturated PDMS (polydimethylsiloxane) material |
| US20180067038A1 (en) * | 2015-03-19 | 2018-03-08 | The Board Of Trustees Of The Leland Stanford Junior University | Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip |
| CN107532990A (en) * | 2015-05-12 | 2018-01-02 | 芯片生物技术株式会社 | Single particle analytic method and the system for the parsing |
| CN109415671A (en) * | 2017-04-10 | 2019-03-01 | 古河电气工业株式会社 | Liquid feeding device and feeding method |
| CN108251269A (en) * | 2017-12-08 | 2018-07-06 | 深圳市博瑞生物科技有限公司 | Digital pcr droplet generating means |
| CN109738632A (en) * | 2019-01-09 | 2019-05-10 | 南京岚煜生物科技有限公司 | Multi objective micro-fluidic chip and its application method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11883824B2 (en) | Assay using different spacing heights | |
| CA2998587C (en) | Device and system for analyzing a sample, particularly blood, as well as methods of using the same | |
| AU2019222844A1 (en) | Device and system for collecting and analyzing vapor condensate, particularly exhaled breath condensate, as well as method of using the same | |
| AU2016304896A1 (en) | Bio/chemical assay devices and methods for simplified steps, small samples, accelerated speed, and ease-of-use | |
| CN101928663B (en) | Integrated fluidic chip device for digital nucleic acid amplification and application | |
| US20090280518A1 (en) | System for high throughput measurement of mechanical properties of cells | |
| CN108220155A (en) | For the system and method for molecule diagnosis | |
| JP2016505302A5 (en) | ||
| CN102277294A (en) | High-density array chip device used for digital nucleic acid amplification application of device | |
| CN101329358A (en) | Sample dispensing treatment apparatus | |
| CN109112063A (en) | A kind of detection of nucleic acids micro-fluidic chip and preparation method thereof | |
| CN103191792B (en) | Microfluidic chip for microspheric multi-element biological detection | |
| Kim et al. | Microfluidic pipette tip for high-purity and high-throughput blood plasma separation from whole blood | |
| CN201901669U (en) | Integrated flow path chip device for digital nucleic acid amplification | |
| CN103293049A (en) | Serum separation method and serum separation device | |
| CN110331083A (en) | A kind of digital pcr chip and its operating method | |
| Kim et al. | A micro-aspirator chip using vacuum expanded microchannels for high-throughput mechanical characterization of biological cells | |
| JP5834124B2 (en) | Microfluidic chip for steam distillation | |
| CN212222935U (en) | Detection system and liquid drop generating device thereof | |
| KR20150107711A (en) | An improved device and method for sample separation | |
| JP2018061451A (en) | Nucleic acid extraction device and nucleic acid extraction method | |
| CN206847996U (en) | Sample is collected and the device of detection | |
| US7473401B1 (en) | Fluidic extraction of microdissected samples | |
| CN202547977U (en) | Fluid sample collection device | |
| CN211111988U (en) | Scraps of paper blood elution device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191015 |
|
| WD01 | Invention patent application deemed withdrawn after publication |