CN110320353B - Fluorescence immunoassay analyzer applied to plateau environment and sample detection method - Google Patents
Fluorescence immunoassay analyzer applied to plateau environment and sample detection method Download PDFInfo
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- CN110320353B CN110320353B CN201910657595.0A CN201910657595A CN110320353B CN 110320353 B CN110320353 B CN 110320353B CN 201910657595 A CN201910657595 A CN 201910657595A CN 110320353 B CN110320353 B CN 110320353B
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Abstract
The invention discloses a fluorescence immunoassay analyzer applied to a plateau environment, which is provided with a sample injection assembly, an instrument lower shell and an instrument upper shell, wherein the instrument lower shell and the instrument upper shell are assembled and enclosed to form an accommodating cavity; the pneumatic and hydraulic pressure control assembly comprises a pneumatic and hydraulic tightening device and a pneumatic and hydraulic pump device and is used for realizing active control of the microfluidic chip; the pneumatic and hydraulic tightening device comprises an external air path interface pressing head and an external liquid path interface pressing head; the air and liquid pump device comprises an air pump and a liquid pump; the air pump is provided with an external air connecting joint and a micro-fluidic chip air connecting joint; the liquid pump is provided with an external liquid connecting joint and a microfluidic chip liquid connecting joint. Due to the arrangement of the gas and hydraulic pressure control assembly, the analyzer and the microfluidic chip can be used in a matched manner in a low-temperature and low-pressure environment, and technical support is provided for the instant diagnosis of diseases in a plateau environment.
Description
Technical Field
The invention belongs to the field of fluorescence immunoassay detection, and particularly relates to a fluorescence immunoassay analyzer applied to a plateau environment and a sample detection method.
Background
The fluorescence immunoassay technology is a detection technology commonly used in biomedical detection at present, and the fluorescence immunoassay technology is characterized in that the analyte is qualitatively and quantitatively detected by utilizing the characteristic that the analyte generates fluorescence under the excitation of specific wavelength light. The fluorescence immunoassay technology has a series of advantages of high sensitivity, strong specificity, high detection speed, safety, stability and the like, so the fluorescence immunoassay technology is widely applied to clinical detection and has wide application prospect in the detection fields of endocrine disease detection, infectious disease detection, gynecological disease detection, tumor marker detection, genetic disease detection, blood and cytology detection and the like.
The traditional fluorescence immunoassay analyzer is usually large in size and inconvenient to use in a handheld manner, so that the development of the traditional fluorescence immunoassay analyzer in the field of emergency detection (such as emergency laboratories, ICUs, pediatrics, 120 emergency vehicles, outdoor emergency rescue and individual rescue), inconvenient traffic and economically undeveloped areas is limited. Even some commercial products have the defects of single detection wave band, complex structure, poor reliability, low precision and the like.
Chinese patent document CN108181456A discloses a handheld fluorescence immunoassay device, which comprises a housing and an upper cover, wherein the housing and the upper cover form a containing cavity, a fluorescence data acquisition module, a photoelectric scanning module and a main control module are arranged in the containing cavity, and a touch screen is arranged on the surface of the upper cover; the fluorescence data acquisition module is arranged above the photoelectric scanning module, and the main control module is respectively connected with the fluorescence data acquisition module, the photoelectric scanning module and the touch screen; the fluorescence data acquisition module comprises a fluorescence data acquisition board, an optical detector and a light path component, and the fluorescence data acquisition board is arranged above the light path component; the optical detector is arranged above the central hole of the light path component and is connected with the fluorescence data acquisition board through a tail pin; the fluorescence data acquisition module also comprises a plurality of excitation light sources, each excitation light source is a light source with different wave bands, and the excitation light sources are respectively positioned in light source holes at the upper part of the light path component; the device also comprises a reagent clamping groove integrated at the bottom of the support of the photoelectric scanning module; the position of the fluorescence data acquisition module in the accommodating cavity is movable.
The fluorescence data acquisition module of the fluorescence immunoassay device in the technical scheme comprises a plurality of excitation light sources which can be respectively light sources of different wave bands, so that the fluorescence immunoassay device is multipurpose, supports the detection of a plurality of fluorescence wave band samples, and expands the application range of the device; the fluorescent data acquisition module is moved and the reagent card is fixed, so that the overall structural layout is compact, the size is small, and the handheld operation and carrying are easy to realize. However, the fluorescence immunoassay device and the immunochromatography reagent strip in the current market are usually used in temperature and pressure environments, and particularly, the low-temperature environment causes that liquid cannot flow smoothly, so that the detection accuracy of the chromatography reagent strip is affected. In order to meet the requirement of immediate detection of disease (such as myocardial injury) markers in a plateau low-temperature low-pressure environment, a fluorescence immunoassay instrument needs to be matched with a microfluidic chip for use, and the microfluidic chip is divided into a passive type and an active type. Wherein: passive microfluidic requires capillary forces to achieve lateral flow of liquid forward. However, the liquid flow rate cannot be uniform due to the different viscosities of different samples, especially whole blood samples. Active micro-fluidic can effectively avoid the above-mentioned problem, can give forward thrust, makes liquid even forward flow, avoids because the test value difference that different velocity of flow lead to. The active micro-fluidic power comprises centrifugal force driving, electrowetting driving and pressure driving (electrolytic pump, compressed gas pump, chemical decomposition pump and direct air pressure difference driving), but if the purpose of randomly controlling the liquid flow is to be achieved, the driving force is required, the valve control is also required, and the backflow prevention structure prevents the liquid from flowing back because of pressure removal; therefore, there is a need for improvement of the existing fluorescence immunoassay apparatus.
Disclosure of Invention
The invention aims to provide a fluorescence immunoassay analyzer applied to a plateau environment.
In order to solve the technical problems, the technical scheme adopted by the invention is that the fluorescence immunoassay analyzer applied to the plateau environment is provided with a sample injection assembly, an instrument lower shell and an instrument upper shell, wherein the instrument lower shell and the instrument upper shell are assembled and enclosed to form an accommodating cavity; the pneumatic and hydraulic pressure control assembly comprises a pneumatic and hydraulic tightening device and a pneumatic and hydraulic pump device and is used for realizing active control of the microfluidic chip;
the pneumatic and hydraulic tightening device comprises an external air path interface pressing head and an external liquid path interface pressing head;
the air and liquid pump device comprises an air pump and a liquid pump; the air pump is provided with an external air connecting joint and a micro-fluidic chip air connecting joint; the liquid pump is provided with an external liquid connecting joint and a microfluidic chip liquid connecting joint.
The gas and hydraulic pressure control assembly is arranged, so that the analyzer is matched with the microfluidic chip in use in a low-temperature and low-pressure environment; the normal operation of gas and liquid in low-temperature and low-pressure environment is realized by using the pressure control of the gas and the liquid.
Preferably, the analyzer also comprises a sample feeding clamping groove temperature control component and a cleaning bottle temperature control component; the sample feeding clamping groove temperature control assembly consists of a temperature control incubation sheet and a first temperature sensor, wherein the temperature control incubation sheet is positioned at the lower part of a clamping groove of the sample feeding assembly; the cleaning bottle temperature control assembly consists of a temperature control sheet on the outer surface of the cleaning liquid bottle accommodating cavity and a second temperature sensor.
The temperature control incubation sheet and the first temperature sensor which are positioned at the lower part of the clamping groove of the sample feeding assembly can realize the heating or refrigerating effect on the liquid in the microfluidic chip; the temperature control piece and the second temperature sensor which are positioned on the outer surface of the containing cavity of the cleaning liquid bottle are used for controlling the temperature of the cleaning liquid, so that the analyzer can not only meet low temperature and low pressure, but also meet the requirement of use in a high-temperature environment through the refrigeration effect of the temperature control assembly.
Preferably, the pneumatic and hydraulic tightening device further comprises a motor I, a motor rotating shaft I and a pressing slide block, and the external gas circuit interface pressing head and the external liquid circuit interface pressing head both penetrate through the pressing slide block; the first motor drives the first screw rod to move, and the pressing sliding block is connected with the first screw rod in a sliding mode and moves along a guide shaft of the pressing sliding block.
Preferably, the analyzer further comprises a conductive rubber micro-valve control assembly, wherein the conductive rubber micro-valve control assembly is provided with a capacitance probe, a motor II, a motor rotating shaft II and a lower pressure head integrally arranged with the motor rotating shaft; the capacitance probe detects the capacitance change of liquid flowing through the microfluidic chip, a signal is fed back to the circuit main board CPU, the motor II is started, the motor rotating shaft II moves downwards, and the lower pressing head presses the conductive rubber micro valve of the microfluidic chip.
Preferably, a photoelectric scanning assembly is further arranged in the accommodating cavity, and the photoelectric scanning assembly is provided with a fluorescence data acquisition assembly; the circuit main board CPU is also arranged in the accommodating cavity.
The photoelectric scanning assembly comprises a fluorescence data acquisition assembly, and belongs to the prior art; the structure and function may be the same as those disclosed in patent document CN108181456A in the background art; the circuit mainboard CPU comprises a CPU and a connecting circuit of each functional component.
Preferably, the clamping groove of the sample feeding assembly is fixedly connected with the sliding block, the sliding block moves along the second screw rod, and the second screw rod is driven by a screw rod motor; the sample feeding assembly further comprises a clamping groove limiting block and a guide rail.
The clamping groove is used for bearing the micro-fluidic chip, and the control of the movement of the micro-fluidic chip is realized through the screw rod motor, the screw rod II, the sliding block and the clamping groove.
Preferably, a limiting optical coupler is arranged in the accommodating cavity, and the clamping groove limiting block stops moving when reaching the limiting optical coupler along the guide rail.
A blood sample is added from a sample filling port of the microfluidic chip and is manually pushed into a sample inlet in a sample injection assembly of the analyzer, the lead screw motor runs after the microfluidic chip reaches the sample injection limit of the clamping groove, the clamping groove is fixedly connected with the sliding block and moves along the second lead screw, and a clamping groove limiting block at the front end of the clamping groove reaches the limit optocoupler and stops moving; the temperature control incubation piece is adhered to the bottom of the clamping groove, so that heating and refrigeration can be realized, the appropriate temperature of a detection system is ensured, particularly, the temperature can be rapidly increased in a low-temperature environment, and the normal operation of detection in the micro-fluidic chip is ensured.
Preferably, the external air connecting joints of the air pump are respectively an air inlet connector and an air outlet connector; the external liquid connection joint of the liquid pump is respectively a cleaning liquid inlet connector and a cleaning liquid outlet connector.
Preferably, the cleaning bottle temperature control assembly comprises a cleaning liquid bottle, a cleaning liquid bottle accommodating cavity and a puncture needle; the puncture needle is arranged in the center of the bottom of the cleaning solution bottle accommodating cavity; the puncture needle is communicated with a cleaning liquid inlet interface of the cleaning liquid bottle accommodating cavity, and the temperature control sheet and the second temperature sensor are arranged on the outer surface of the cleaning liquid bottle accommodating cavity.
In this application, the temperature control piece has contained heating plate and refrigeration piece, and the temperature control piece setting is at holding intracavity surface.
Preferably, a display screen is further arranged on the instrument upper shell.
The invention also provides a sample detection method applied to the plateau environment, which comprises the following steps:
(1) the analyzer starts self-checking, operates according to the prompt of a display screen, adds a cleaning liquid bottle into the analyzer, adds a blood sample from a sample filling port of the microfluidic chip, manually pushes the sample filling port of the analyzer, runs a screw motor after the microfluidic chip reaches the sample filling limit of a clamping groove, the clamping groove is fixedly connected with a sliding block and moves along a screw rod II, and a clamping groove limiting block reaches a limit optocoupler and stops moving;
(2) the method comprises the following steps that a first motor of the pneumatic and hydraulic tightening device is started, a first motor of the pneumatic and hydraulic tightening device drives a first screw rod to move, a first compression slide block and a first screw rod are connected in a sliding mode and move along guide shafts of two compression slide blocks, two compression heads are respectively in contact compression with a sample filling port of a microfluidic chip and a compression part externally connected with a cleaning liquid inlet, then air enters through an air inlet on the outer side face of an analyzer, enters through an air inlet port of an air pump, is compressed and pressurized through the air pump, enters the microfluidic chip from an air outlet port of the air pump; the cleaning liquid is provided by a replaceable cleaning liquid bottle, the cleaning liquid bottle is arranged in a cleaning liquid bottle accommodating cavity where the temperature control sheet is adhered to the temperature sensor, then enters the liquid pump through the liquid inlet interface, and finally is connected with an external liquid path connecting port of the microfluidic chip through the liquid outlet interface of the liquid pump, so that the addition of the cleaning liquid of the microfluidic chip is realized;
(3) the working process of the conductive rubber micro valve comprises the following steps: the capacitance detection part detects the capacitance change in the conductive rubber micro valve, a motor II is started, a rotating shaft of the motor is communicated with a lower pressure head and moves downwards to press the valve of the conductive rubber micro valve of the microfluidic chip, so that interception is realized, and after the reaction is finished, when the valve of the conductive rubber micro valve of the microfluidic chip is opened, liquid enters the waste liquid cavity;
(4) the analyzer controls the flow of a sample in the microfluidic chip through the pneumatic and hydraulic pressure control assembly, the reaction is uniformly mixed, the cleaning solution is injected and flows, and the conductive rubber micro valve is opened and closed, so that the active control on the microfluidic chip is realized, the reacted fluorescent substance is finally collected, subjected to signal conversion amplification processing and data analysis through the photoelectric scanning assembly, and the detection result of the measurement index is displayed on the display screen of the analyzer.
Drawings
The following further detailed description of embodiments of the invention is made with reference to the accompanying drawings:
FIG. 1 is a schematic view showing the overall structure of a fluorescence immunoassay analyzer for use in a plateau environment according to the present invention;
FIG. 2 is a schematic side view of a fluorescence immunoassay analyzer for use in a plateau environment according to the present invention;
FIG. 3 is a schematic diagram of the internal structure of the fluorescence immunoassay analyzer applied to a plateau environment according to the present invention;
FIG. 4 is a schematic of the top view of FIG. 3;
FIG. 5 is a schematic diagram of a sample injection assembly of the fluorescence immunoassay analyzer applied to a plateau environment according to the present invention;
FIG. 6 is a schematic side view of the pneumatic and hydraulic tightening mechanism of the pneumatic and hydraulic force control assembly;
FIG. 7 is a schematic perspective view of the pneumatic and hydraulic tightening device of the pneumatic and hydraulic force control assembly;
FIG. 8 is a schematic perspective view of the pneumatic and hydraulic pump assembly of the pneumatic and hydraulic force control assembly;
FIG. 9 is a schematic perspective view of a temperature control assembly of a cleaning bottle of the fluorescence immunoassay analyzer for use in a plateau environment according to the present invention;
FIG. 10 is a schematic view showing the effect of the cleaning solution bottle of FIG. 9 after being installed;
FIG. 11 is a schematic diagram of the front structure of the microfluidic chip;
FIG. 12 is a schematic perspective view of a microfluidic chip;
wherein: 1-sample introduction assembly; 101-a card slot; 102-a slide block; 103-a second screw rod; 104-a screw motor; 105-a slot stopper; 106-a guide rail; 2-instrument lower shell; 3-instrument upper shell; 4-a holding cavity; 5-pneumatic and hydraulic pressure control assembly; 6-pneumatic and hydraulic tightening devices; 601-external gas circuit interface pressing head; 602-external liquid circuit interface pressing head; 603-motor one; 604-motor shaft one; 605-a compacting slide block; 606-screw rod one; 607. 608-pressing the slide block guide shaft; 7-gas, liquid pump device; 701-an air pump; 702-a liquid pump; 703-connecting an external gas connection joint; 704-external liquid connection joint; 705-air inlet connection; 706-air outlet connection; 707-cleaning liquid inlet connection; 708-a cleaning liquid outlet connection; 8-a sample feeding clamping groove temperature control component; 801-temperature controlled incubation sheet; 802-a first temperature sensor; 9-cleaning bottle temperature control component; 901-temperature control sheet; 902-a second temperature sensor; 903-cleaning solution bottle; 904-cleaning liquid inlet interface; 905-puncture needle; 906-cleaning solution bottle holding chamber; 10-conductive rubber micro-valve control component; 1001-capacitive probe; 1002-motor two; 1003-motor shaft two; 1004-lower ram; 11-circuit board CPU; 12-a microfluidic chip; 1201-conductive rubber microvalves; 1202-a conductive rubber micro-valve pressing part; 1203-a conductive rubber micro valve capacitance detection part; 1204-external connection liquid path connector; 1205-external gas circuit connector; 1206-sample fill port; 13-a photoelectric scanning assembly; 14-limit optocouplers; 15-a display screen; 16-cleaning bottle replacement position; 17-air inlet.
Detailed Description
As shown in fig. 1 and 2, the fluorescence immunoassay analyzer applied to plateau environment of the present invention comprises a sample injection assembly 1, an instrument lower shell 2 and an instrument upper shell 3, wherein the instrument upper shell 2 is further provided with a display screen 15; the instrument lower shell 2 and the instrument upper shell 3 are assembled to form an accommodating cavity 4, and a pneumatic and hydraulic pressure control assembly 5 is arranged in the accommodating cavity 4; the pneumatic and hydraulic pressure control assembly 5 comprises a pneumatic and hydraulic tightening device 6 and a pneumatic and hydraulic pump device 7 (as shown in fig. 8) for realizing active control of the microfluidic chip 12; the gas and hydraulic tightening device 6 comprises an external gas path interface pressing head 601 and an external liquid path interface pressing head 602;
the air and liquid pump device 7 comprises an air pump 701 and a liquid pump 702; the air pump 701 is provided with an external air connection joint 703 and a microfluidic chip air connection joint (not shown in the drawing); the liquid pump has an external liquid connection connector 704 and a microfluidic chip liquid connection connector (not shown in the drawings).
As shown in fig. 3 and 4, the analyzer further comprises a sample feeding slot temperature control assembly 8 and a cleaning bottle temperature control assembly 9; the sample feeding slot temperature control component 8 consists of a temperature control incubation sheet 801 and a first temperature sensor 802 which are positioned at the lower part of the slot 101 of the sample feeding component 1; the cleaning bottle temperature control assembly 9 is composed of a temperature control sheet 901 on the outer surface of the cleaning liquid bottle accommodating cavity 906 and a second temperature sensor 902.
The pneumatic and hydraulic tightening device 6 further comprises a first motor 603, a first motor rotating shaft 604 and a pressing slide block 605, as shown in fig. 6 and 7, the external gas circuit interface pressing head 601 and the external liquid circuit interface pressing head 602 both penetrate through the pressing slide block 605; the first motor 603 drives the first screw rod 606 to move, and the pressing sliding block 605 is connected with the first screw rod 606 in a sliding mode and moves along the guide shafts 607 and 608 of the pressing sliding block.
The analyzer of the present embodiment further includes a conductive rubber micro valve control assembly 10 (as shown in fig. 4), which is provided with a capacitance probe 1001, a second motor 1002, a second motor rotating shaft 1003, as shown in fig. 7, and a lower pressure head 1004 integrally arranged with the second motor rotating shaft 1003; the capacitance probe 1001 detects the capacitance change of the liquid in the microfluidic chip 12, feeds back a signal to the circuit main board CPU 11, starts the second motor 1002, the second motor shaft 1003 moves downward, and the lower pressure head 1004 presses the conductive rubber microvalve 1201 of the microfluidic chip 12, as shown in fig. 11.
A photoelectric scanning component 13 is also arranged in the accommodating cavity 4, and the photoelectric scanning component 13 is provided with a fluorescence data acquisition component; the circuit board CPU 11 is also disposed in the accommodation cavity 4.
As shown in fig. 5, a clamping groove 101 of the sample feeding assembly 1 is fixedly connected with a sliding block 102, the sliding block 102 moves along a second lead screw 103, and the second lead screw 103 is driven by a lead screw motor 104; the sample feeding assembly 1 further comprises a clamping groove limiting block 105 and a guide rail 106; a limiting optical coupler 14 is arranged in the accommodating cavity 4, and the clamping groove limiting block 105 stops moving when reaching the limiting optical coupler 14 along the guide rail 106.
As shown in fig. 8, the external air connectors 703 of the air pump 701 are respectively an air inlet connector 705 and an air outlet connector 706; the external connection joints 704 of the liquid pump 702 are respectively a cleaning liquid inlet joint 707 and a cleaning liquid outlet joint 708.
The cleaning bottle temperature control assembly 9 comprises a cleaning liquid bottle 903, a cleaning liquid inlet interface 904, a cleaning liquid bottle accommodating cavity 906 and a puncture needle 905, as shown in fig. 9 and 10; the puncture needle 905 is arranged in the center of the bottom of a cleaning solution bottle accommodating cavity 906 in which the cleaning solution bottle 903 is clamped; the puncture needle 905 is communicated with a cleaning liquid inlet interface 904 of the cleaning liquid bottle accommodating cavity 906, and the temperature control sheet 901 (including a heating sheet and a refrigerating sheet) and the second temperature sensor 902 are both arranged on the outer surface of the cleaning liquid bottle accommodating cavity 906.
The micro-fluidic chip 12 in this embodiment includes a conductive rubber micro-valve 1201, a conductive rubber micro-valve pressing part 1202, a conductive rubber micro-valve capacitance detection part 1203, an external liquid path connection port 1204, an external gas path connection port 1205, and a sample filling port 1206.
Based on the fluorescence immunoassay analyzer applied to the plateau environment provided by the embodiment, the sample detection method applied to the plateau environment of the embodiment comprises the following steps:
(1) the analyzer starts self-checking, operates according to the prompt of a display screen, a cleaning liquid bottle 903 is added into the analyzer, a blood sample is added from a sample filling port 1206 of the microfluidic chip 12 and is manually pushed into the sample inlet of the analyzer (in a sample injection assembly 1), the microfluidic chip 12 reaches the sample injection limit of the clamping groove 101, then the lead screw motor 104 operates, the clamping groove 101 is fixedly connected with the sliding block 102 and moves along the second lead screw 103, and the clamping groove limiting block 105 reaches the limit optocoupler 14 and stops moving;
the bottom of the clamping groove 101 is adhered with a temperature control incubation sheet 801 and a first temperature sensor 802, so that heating and refrigeration can be realized, the appropriate temperature of a detection system is ensured, particularly, the temperature can be rapidly increased in a low-temperature environment, and the normal operation of detection in the microfluidic chip 12 is ensured;
(2) a first motor 603 of the pneumatic and hydraulic tightening device 6 is started, a first motor 603 of the pneumatic and hydraulic tightening device 6 drives a first screw rod 606 to move, a tightening slide block 605 is connected with the first screw rod 606 in a sliding manner and moves along two tightening slide block guide shafts 607 and 608, two tightening heads (namely an external air path interface tightening head 601 and an external liquid path interface tightening head 602) are respectively in contact and tightening with a sample filling port 1206 of the microfluidic chip 12 and an external tightening part of a cleaning liquid inlet, then air enters through an air inlet 17 on the outer side surface of the analyzer, enters through an air inlet interface 705 (namely an air inlet connector) of an air pump 701, is compressed and pressurized by the air pump 701, enters the microfluidic chip 12 from an air outlet interface 706 (namely an air outlet connector) of the air pump to drive a sample; cleaning liquid is provided by a replaceable cleaning liquid bottle, the cleaning liquid bottle is arranged in a cleaning liquid bottle accommodating cavity 906 which is adhered to the temperature control sheet 901 and the second temperature sensor 902, then enters the liquid pump 702 through a liquid inlet interface 707 (namely a cleaning liquid inlet connector), and finally is connected with an external liquid path connecting port 1204 of the microfluidic chip 12 through a liquid outlet interface 708 (namely a cleaning liquid outlet connector) of the liquid pump, so that the addition of the cleaning liquid into the microfluidic chip 12 is realized;
(3) the working process of the conductive rubber micro valve 1201 is as follows: the capacitance detection part 1203 detects the capacitance change in the conductive rubber micro valve 1201, a second motor 1002 is started, a second motor rotating shaft 1003 is communicated with a lower pressure head 1004 and moves downwards to press the valve of the conductive rubber micro valve 1201 of the microfluidic chip 12, so that cut-off is realized, and after the reaction is finished, when the valve of the conductive rubber micro valve 1201 of the microfluidic chip 12 is opened, liquid enters a waste liquid cavity;
(4) the analyzer controls the flow of the sample in the microfluidic chip 12 through the pneumatic and hydraulic pressure control assembly 5, the reaction is uniformly mixed, the injection and the flow of the cleaning solution are realized, the opening and the closing of the conductive rubber micro valve 1201, the active control on the microfluidic chip 12 is realized, finally, the reacted fluorescent substance is collected, the signal is converted and amplified and processed, the data is analyzed through the photoelectric scanning assembly 13, and the detection result of the measurement index is displayed on the display screen 15 of the analyzer.
It should be noted that, in this embodiment, the cleaning liquid bottle 903 is replaceable, the puncture site at the bottom of the bottle is made of rubber, and when the cleaning bottle is replaced, the puncture needle 905 is pulled out to ensure that the cleaning liquid does not flow out.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A fluorescence immunoassay analyzer applied to plateau environment is provided with a sample injection assembly, an instrument lower shell and an instrument upper shell, wherein the instrument lower shell and the instrument upper shell are assembled and enclosed to form an accommodating cavity; the pneumatic and hydraulic pressure control assembly comprises a pneumatic and hydraulic tightening device and a pneumatic and hydraulic pump device and is used for realizing active control of the microfluidic chip;
the pneumatic and hydraulic tightening device comprises an external air path interface pressing head and an external liquid path interface pressing head;
the air and liquid pump device comprises an air pump and a liquid pump; the air pump is provided with an external air connecting joint and a micro-fluidic chip air connecting joint; the liquid pump is provided with an external liquid connecting joint and a microfluidic chip liquid connecting joint;
the analyzer also comprises a sample feeding clamping groove temperature control component and a cleaning bottle temperature control component; the sample feeding clamping groove temperature control assembly consists of a temperature control incubation sheet and a first temperature sensor, wherein the temperature control incubation sheet is positioned at the lower part of a clamping groove of the sample feeding assembly; the cleaning bottle temperature control assembly consists of a temperature control sheet on the outer surface of the cleaning liquid bottle accommodating cavity and a second temperature sensor;
the pneumatic and hydraulic tightening device also comprises a motor I, a motor rotating shaft I and a tightening slide block, wherein the external gas circuit interface tightening head and the external liquid circuit interface tightening head both penetrate through the tightening slide block; the first motor drives the first screw rod to move, and the pressing sliding block is connected with the first screw rod in a sliding mode and moves along a guide shaft of the pressing sliding block;
the analyzer also comprises a conductive rubber micro-valve control assembly, wherein the conductive rubber micro-valve control assembly is provided with a capacitance probe, a motor II, a motor rotating shaft II and a lower pressure head which is integrated with the motor rotating shaft; the capacitance probe detects the capacitance change of liquid flowing through the microfluidic chip, a signal is fed back to the circuit main board CPU, the motor II is started, the motor rotating shaft II moves downwards, and the lower pressing head presses the conductive rubber micro valve of the microfluidic chip.
2. The fluoroimmunoassay analyzer for plateau environments as recited in claim 1, further comprising a photoelectric scanning assembly disposed in the receiving cavity, the photoelectric scanning assembly having a fluorescence data collecting assembly; the circuit main board CPU is also arranged in the accommodating cavity.
3. The fluorescence immunoassay analyzer applied to plateau environment of claim 2, wherein the slot of the sample feeding assembly is fixedly connected with a slide block, the slide block moves along a second screw rod, and the second screw rod is driven by a screw rod motor; the sample feeding assembly further comprises a clamping groove limiting block and a guide rail.
4. The fluoroimmunoassay analyzer for plateau environments as claimed in claim 3, wherein a limiting optical coupler is disposed in the receiving cavity, and the slot limiting block stops moving when reaching the limiting optical coupler along the guide rail.
5. The fluoroimmunoassay analyzer for use in a plateau environment of claim 4, wherein the external air connection connectors of the air pump are an air inlet connector and an air outlet connector, respectively; the external liquid connection joint of the liquid pump is respectively a cleaning liquid inlet connector and a cleaning liquid outlet connector.
6. The fluorescence immunoassay instrument applied to the plateau environment as claimed in claim 5, wherein the cleaning bottle temperature control assembly comprises a cleaning liquid bottle, a cleaning liquid bottle accommodating chamber and a puncture needle; the puncture needle is arranged in the center of the bottom of the cleaning solution bottle accommodating cavity; the puncture needle is communicated with a cleaning liquid inlet interface of the cleaning liquid bottle accommodating cavity, and the temperature control sheet and the second temperature sensor are arranged on the outer surface of the cleaning liquid bottle accommodating cavity.
7. The fluoroimmunoassay analyzer for plateau environments of claim 6, wherein a display screen is further disposed on the upper casing of the analyzer.
8. A sample detection method using the fluoroimmunoassay analyzer for a plateau environment according to any one of claims 1 to 7, comprising the steps of:
(1) the analyzer starts self-checking, operates according to the prompt of a display screen, adds a cleaning liquid bottle into the analyzer, adds a blood sample from a sample filling port of the microfluidic chip, manually pushes the sample filling port of the analyzer, runs a screw motor after the microfluidic chip reaches the sample filling limit of a clamping groove, the clamping groove is fixedly connected with a sliding block and moves along a screw rod II, and a clamping groove limiting block reaches a limit optocoupler and stops moving;
(2) the method comprises the following steps that a first motor of the pneumatic and hydraulic tightening device is started, a first motor of the pneumatic and hydraulic tightening device drives a first screw rod to move, a first compression slide block and a first screw rod are connected in a sliding mode and move along guide shafts of two compression slide blocks, two compression heads are respectively in contact compression with a sample filling port of a microfluidic chip and a compression part externally connected with a cleaning liquid inlet, then air enters through an air inlet on the outer side face of an analyzer, enters through an air inlet port of an air pump, is compressed and pressurized through the air pump, enters the microfluidic chip from an air outlet port of the air pump; the cleaning liquid is provided by a replaceable cleaning liquid bottle, the cleaning liquid bottle is arranged in a cleaning liquid bottle accommodating cavity where the temperature control sheet is adhered to the second temperature sensor, then enters the liquid pump through the liquid inlet interface, and finally is connected with an external liquid path connecting port of the microfluidic chip through the liquid outlet interface of the liquid pump, so that the addition of the cleaning liquid of the microfluidic chip is realized;
(3) the working process of the conductive rubber micro valve comprises the following steps: the capacitance detection part detects the capacitance change in the conductive rubber micro valve, a motor II is started, a rotating shaft of the motor is communicated with a lower pressure head and moves downwards to press the valve of the conductive rubber micro valve of the microfluidic chip, so that interception is realized, and after the reaction is finished, when the valve of the conductive rubber micro valve of the microfluidic chip is opened, liquid enters the waste liquid cavity;
(4) the analyzer controls the flow of a sample in the microfluidic chip through the pneumatic and hydraulic pressure control assembly, the reaction is uniformly mixed, the cleaning solution is injected and flows, and the conductive rubber micro valve is opened and closed, so that the active control on the microfluidic chip is realized, the reacted fluorescent substance is finally collected, subjected to signal conversion amplification processing and data analysis through the photoelectric scanning assembly, and the detection result of the measurement index is displayed on the display screen of the analyzer.
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| US7425310B2 (en) * | 2004-01-29 | 2008-09-16 | Bryan Truex | Apparatus, system, and method of detecting an analyte utilizing pyroelectric technology |
| US8962258B2 (en) * | 2008-08-29 | 2015-02-24 | Korea Advanced Institute Of Science And Technology | Apparatus and method for multiple immunoassays on a chip |
| KR20150097764A (en) * | 2012-12-21 | 2015-08-26 | 마이크로닉스 인코포레이티드. | Portable fluorescence detection system and microassay cartridge |
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