CN110308145A - A kind of chlamydia trachomatis glycogen detection reagent, chlamydia trachomatis glycogen test strip and preparation method thereof - Google Patents
A kind of chlamydia trachomatis glycogen detection reagent, chlamydia trachomatis glycogen test strip and preparation method thereof Download PDFInfo
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Abstract
A kind of chlamydia trachomatis glycogen detection reagent, chlamydia trachomatis glycogen test strip and preparation method thereof, belong to external diagnosis reagent field.The reagent includes substrate, buffer, developing solution, auxiliary reagent；Substrate is phenol, phenol derivatives, naphthylamines, naphthylamine derivative or anthracyclinone derivatives；Developing solution includes principal component and inoxidizability ingredient, and principal component is the dilute sulfuric acid aqueous solution less than or equal to 70%, and inoxidizability ingredient is sodium thiosulfate, ascorbic acid, thiocarbamide, hydrogen peroxide or urea peroxide；Auxiliary reagent includes stabilizer, reducing agent and dispersing agent.The present invention fast, high sensitivity, the advantage of high specificity with detection speed.Specificity of the invention is 96.5-98.6%, sensibility 84.8-87.5%, positive predictive value 90.5%, and negative predictive value 85.1% has good consistency in clinical diagnosis, and chlamydia trachomatis glycogen test paper detection accuracy can reach 99.45%.
Description
Technical field
The invention belongs to technical field of in vitro diagnostic reagents, and in particular to a kind of chlamydia trachomatis glycogen detection reagent, sand
Chlamydia oculogenitale glycogen test strip and preparation method thereof.
Background technique
Chlamydia trachomatis (Ct) is the pathogen of special sexual cell endoparasitism, can cause the propagation of trachoma and venereal disease, is passed in property
The influence broadcast in disease (STD) is increasingly significant, has been more than stranguria syndrome, becomes the highest STD of global disease incidence, urinates in non-gonococcal
Account for about 40-60% in road inflammation (NUG), Male urethritis, prostatitis, epididymitis, womb neck inflammation endometrium can be caused
Inflammation, salpingitis, pelvic inflammatory disease lead to infertile, ectopic pregnancy and miscarriage etc..It, also can nothing clinically mostly in concealment development
There are light symptoms without being paid attention to by patient in symptom.It is reported that 50% male and 70% Female-cases are in asymptomatic sense
Dye, and sustainable 1 year of symptomless infection is to the several years.Therefore, the routine inspection of chlamydia trachomatis has important clinical medicine meaning.
The diagnosis of chlamydia trachomatis (Ct) there are two main classes method, the first kind is histocyte cultivation, more traditional
Diagnostic method, in view of its very high sensitivity and very strong specificity, cell culture method is still ideal inspection in some cases
Survey method, but this method heavy workload, it is desirable that there are specialized facilities and skilled engineer, time-consuming and somewhat expensive, and
It is only applicable to cotton swab sample, cannot achieve the purpose of quick diagnosis, because time-consuming for it, and the sensibility of different experiments room cultivation
Also not identical (50-90%), part sample Chlamydia death causes the false negative of culture when culture, is not able to satisfy clinical need
It asks；Second class is acellular cultural method, relatively broad at present to be used for clinical or epidemiological survey detection means including straight
Connect fluorescent antibody technics (DFA), enzyme immunoassay (EIA) (EIA) and polymerase chain reaction (PCR) technology etc..Polymerase chain reaction
(PCR) technology is had been developed in recent years, is the breakthrough of molecular techniques, it is natural in artificial condition Imitating
DNA replication dna process carries out rapid amplifying to specific DNA fragments, and the product after amplification can be under analyzer visually as it can be seen that this method
High sensitivity, high specificity, but the competency profiling of the quality and technical staff to required reagent is high, dedicated molecular biosciences
Laboratory, experiment condition is more demanding, need to post-process to PCR product, not only time-consuming, and is easy cross contamination, causes to examine
Result false positive is surveyed, while may cause false positive rate and false negative rate potentially hazardous, and that have high to experimenter, and
There are different infection rates for different crowd, so that the detection efficiency difference of various detection methods, is unable to reach uniformity, limits
Its development in basic hospital.Enzyme immunoassay (EIA) (EIA) is quick, easy to operate, has higher specificity, but sensitivity is low, holds
Easily cause missing inspection.Using direct fluorescence antibody (DFA) detection system, detector can be made from the same detection plate or each reaction tube
Up to 4 fluorescence are collected, and the signal of each fluorescence can be distinguished, can at most monitor 4 PCR in a reaction tube simultaneously
Amplified reaction.Clinically in patient, NG, Ct and UP are often in co-infection state, but currently used detection method still cannot essence
Really detection and identification.
Summary of the invention
In order to solve the problems, such as that the existing detection for chlamydia trachomatis exists, the present invention provides a kind of chlamydia trachomatis sugar
Former detection reagent, chlamydia trachomatis glycogen test strip and preparation method thereof.
Used technical solution is as follows in order to solve the technical problem by the present invention:
A kind of chlamydia trachomatis glycogen detection reagent of the invention, comprising: substrate, buffer, developing solution, auxiliary reagent；
The substrate is phenol, phenol derivatives, naphthylamines, naphthylamine derivative or anthracyclinone derivatives；
The developing solution includes principal component and inoxidizability ingredient, and the principal component is the dilute sulfuric acid less than or equal to 70%
Aqueous solution, the inoxidizability ingredient are sodium thiosulfate, ascorbic acid, thiocarbamide, hydrogen peroxide or urea peroxide；
The auxiliary reagent includes stabilizer, reducing agent and dispersing agent.
As preferred embodiment, the concentration of the substrate is 1~200mg/mL.
As preferred embodiment, the substrate is selected from phenol, 2- methylphenol, 4- methylphenol, 2- chlorophenol, 4-
Chlorophenol, 2- bromophenol, 4- bromophenol, anthrone, 2- methyl anthrone, 4- methyl anthrone, 2- chrloroanthracene ketone, 4- chrloroanthracene ketone, 2- bromine anthracene
One of ketone, 4- bromine anthrone, 5- methyl anthrone, 7- methyl anthrone, 5- chrloroanthracene ketone, 5- bromine anthrone.
As preferred embodiment, the pH of the buffer is 5~10, and concentration is 100~500mM, is selected from phosphoric acid hydrogen
Disodium-citrate buffer solution, acetic acid-sodium acetate buffer solution, disodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution, disodium hydrogen phosphate-phosphorus
One of acid dihydride potassium buffer, potassium dihydrogen phosphate-sodium hydrate buffer solution, barbital sodium-hydrochloride buffer are a variety of.
As preferred embodiment, in the developing solution, the volume of principal component is 100mL, inoxidizability ingredient it is dense
Degree is 1~100mg/mL.
As preferred embodiment, the concentration of the stabilizer is 5~20mg/mL, is selected from cystine, leucine, ox
One of seralbumin, L-cysteine are a variety of.
As preferred embodiment, the concentration of the reducing agent are as follows: 1.00~100.0mg/mL is selected from thiosulfuric acid
One of sodium, ascorbic acid, thiocarbamide, hydrogen peroxide or urea peroxide are a variety of.
As preferred embodiment, the concentration of the dispersing agent is 1~20mg/mL, selected from PVP-K90, PEG-2000,
One of PEG-4000, PEG-6000 or a variety of.
A kind of chlamydia trachomatis glycogen test strip of the invention contains a kind of above-mentioned chlamydia trachomatis glycogen detection
Reagent.
A kind of preparation method of chlamydia trachomatis glycogen test strip of the invention, mainly comprises the steps that
Step 1: preparating liquid
Buffer and auxiliary reagent are uniformly mixed, substrate is added and is configured to medical fluid；
Step 2: dry
It is dried using soak drying machine automation drying means；
The setting of soak drying machine temperature is as follows:
Main heating temperature is set as 75~85 DEG C after booting, secondary heating temperature is set as 55~75 DEG C, to main heating temperature
Degree reaches 75~85 DEG C, and filter paper is soaked into the liquid beginning immersion liquid when each dry room temperature >=75 DEG C；
The setting of soak drying machine walking speed is as follows:
6.0~12.0Hz.
The beneficial effects of the present invention are:
The present invention is substrate according to phenol and its derivatives, and in view of in microecology in vaginas environment, there are chlamydia trachomatis sugar
Original, under strongly acidic conditions, glycogen dehydration generate furfural derivatives (furfural and hydroxymethylfurfural etc.), send out with phenol analog derivative
Raw color reaction, it is directly proportional to chlamydia trachomatis glycogen content in color depth, dry type test strip is prepared into as principle.
Preparation of reagents of the invention is simple and efficient, easy to use, easy to operate, does not need when detection that microscope etc. is special to be set
It is standby, in conjunction with the full-automatic Gynecological secretion analysis system of the auspicious GMD-S600 of enlightening, can go out in 8-10min as a result, having detection speed
Fast, high sensitivity, the advantage of high specificity are spent, the defect blank of market product can be filled up, it is easy to operate compared with similar products,
It is suitable for universal.
According to data statistics: a kind of chlamydia trachomatis glycogen detection reagent of the invention, specificity is 96.5-98.6%, quick
Perception is 84.8-87.5%, and positive predictive value 90.5%, negative predictive value 85.1% has good consistency in clinical diagnosis,
Chlamydia trachomatis glycogen test paper detection accuracy can reach 99.45%, becomes a kind of and quickly identifies chlamydia trachomatis vaginitis the most
Accurate detection method fills up the blank of market chemical method detection.
Specific embodiment
A kind of chlamydia trachomatis glycogen detection reagent of the invention mainly includes substrate, buffer, developing solution, auxiliary examination
Agent.
The concentration of described substrate be 1~200mg/mL, substrate be phenol, phenol derivatives, naphthylamines, naphthylamine derivative or
Anthracyclinone derivatives etc., specifically selected from phenol, 2- methylphenol, 4- methylphenol, 2- chlorophenol, 4- chlorophenol, 2- bromophenol,
4- bromophenol, anthrone, 2- methyl anthrone, 4- methyl anthrone, 2- chrloroanthracene ketone, 4- chrloroanthracene ketone, 2- bromine anthrone, 4- bromine anthrone, 5- first
One of base anthrone, 7- methyl anthrone, 5- chrloroanthracene ketone, 5- bromine anthrone etc..
Substrate in formula has high sensitivity to chlamydia trachomatis glycogen.
The pH of described buffer be 5~10 (preferably 6~8, most preferably 7), concentration be 100~500mM, be selected from phosphorus
Sour disodium hydrogen-citrate buffer solution, acetic acid-sodium acetate buffer solution, disodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution, phosphoric acid hydrogen two
One of sodium-potassium phosphate buffer, potassium dihydrogen phosphate-sodium hydrate buffer solution, barbital sodium-hydrochloride buffer etc. or
It is a variety of.
The method is a step immersion method, easy to operate, saving working hour, high sensitivity, the cheap Yi Peiyi of buffer in formula
, buffer can provide a good reaction environment for reaction system.
Described developing solution includes principal component and inoxidizability ingredient, and the volume of principal component is 100mL, principal component be less than
Or the dilute sulfuric acid aqueous solution equal to 70%, the concentration of inoxidizability ingredient are 1~100mg/mL, inoxidizability ingredient is thio sulphur
Sour sodium, ascorbic acid, thiocarbamide, hydrogen peroxide or urea peroxide etc..
Additional developing solution cooperation chlamydia trachomatis glycogen drying chemical reagent paper diagnoses chlamydia trachomatis sugar in secretion jointly
Ultimate constituent.Principal component is 100ml, the dilute sulfuric acid aqueous solution less than or equal to 70% is the critical substances for aoxidizing glycogen；Antioxygen
The property changed ingredient is that sodium thiosulfate, ascorbic acid, thiocarbamide, hydrogen peroxide or urea peroxide etc. are to improve the test paper uniformity and stabilization
Property provide a good reaction environment, and greatly improve developing solution storage effect the phase.
Described auxiliary reagent includes stabilizer, reducing agent and dispersing agent.
The concentration of described stabilizer is 5~20mg/mL, is selected from cystine, leucine, bovine serum albumin(BSA) (BSA), L-
One of cysteine etc. is a variety of.
Described reducing agent, concentration are as follows: 1.00~100.0mg/mL is selected from sodium thiosulfate, ascorbic acid, thiocarbamide, mistake
One of hydrogen oxide or urea peroxide are a variety of.
The concentration of described dispersing agent is 1~20mg/mL, is selected from PVP-K90, PEG-2000, PEG-4000, PEG-6000
Deng one of or it is a variety of.
Stabilizer and reducing agent can greatly prolong the storage capacity of test paper in formula, help to improve the accurate of test paper
Degree, and the dispersion that can increase test paper is used alone or in combination in dispersing agent PVP-K90, PEG-2000, PEG-4000, PEG-6000
Ability facilitates being uniformly distributed for color.
A kind of chlamydia trachomatis glycogen test strip of the invention contains a kind of above-mentioned chlamydia trachomatis glycogen detection
Reagent.
A kind of preparation method of chlamydia trachomatis glycogen test strip of the invention, mainly comprises the steps that
Step 1: preparating liquid
Buffer and auxiliary reagent are uniformly mixed, substrate is added and is configured to medical fluid；
Step 2: dry
It is dried using soak drying machine automation drying means；
The setting of soak drying machine temperature is as follows:
Main heating temperature is set as 75~85 DEG C after booting, secondary heating temperature is set as 55~75 DEG C, to main heating temperature
Degree reaches 75~85 DEG C, and filter paper is soaked into the liquid beginning immersion liquid when each dry room temperature >=75 DEG C；
The setting of soak drying machine walking speed is as follows:
6.0~12.0Hz.The setting of soak drying machine walking speed is gradually confirmed in verification process according to test paper, walking speed
Speed will affect the degree of drying of test paper, have certain influence to the appearance of test paper and accuracy.
Wherein, filter paper type is 7218 type filter paper, 31ET type filter paper, 3MM type filter paper, 526 type filter paper or 319 type filter paper
Deng.
Soak drying machine automation drying means of the present invention has convenient, time saving, automatable advantage, has
Human error bring influences in effect control test paper preparation process, and the heating of soak drying machine major-minor is monitored temperature comprehensively,
Temperature is set within the limits prescribed, can greatly reduce the error between test paper batch, guarantees the accuracy, steady of test paper preparation
Qualitative and homogeneity.
Inventive principle of the invention is as follows:
It is confirmed by deeply probing into chlamydia trachomatis and human body dependence, and by data in literature, chlamydia trachomatis exists
Energy glycogen biosynthesis of each stage (substance, initial body, intermediate) in growth cycle, but it is most common with substance.Bacterium parasitic host
And chlamydia trachomatis glycogen intracellular is generated, chlamydia trachomatis the glycogen PAS is since there are cytochromes chlamydia trachomatis sugar for bacterium
In original system, and chlamydia trachomatis can itself glycogen biosynthesis will detect that a large amount of especially in the inclusion body that proliferation is formed
Glycogen accumulation there are the characteristics of, through confirmation this glycogen from chlamydia trachomatis itself, rather than host cell, therefore pass through
Measurement to glycogen can determine chlamydia trachomatis content in cervical epithelial cells to be tested, and the positive occur and then represent trachoma
Choamydiae infection may further infer infected degree, assist the diagnosis of chlamydia trachomatis infection.
Energy glycogen biosynthesis of usual each stage (substance, initial body, intermediate) of the chlamydia trachomatis in growth cycle, bacterium
Parasitic host simultaneously generates chlamydia trachomatis glycogen intracellular, and chlamydia trachomatis the glycogen PAS is since there are cytochromes trachomas for bacterium
In Chlamydia glycogen system, especially in the inclusion body that proliferation is formed, a large amount of glycogen accumulation will detect that, not due to glycogen
It is dissolved in ethyl alcohol, therefore precipitates glycogen with dehydrated alcohol.Dense H2SO4Glycogen dehydration is set to generate furfural derivatives, at this time according to bottom used
Object is different, and there is some difference for method.The latter acts on forming pink, blue-green compound again with derivatives such as phenol, anthrones,
Intuitively it can be observed how the different colour developing depth of carbohydrate from phenol reagent is different, fructose colour developing is most deep, and glucose takes second place, gala
Sugar, mannose are shallower, and pentose colour developing is most shallow.Chlamydia trachomatis glycogen test paper testing principle (is using 2- methylphenol as substrate
Example):
Chlamydia trachomatis parasitizes in host cell, using itself glycogen biosynthesis system, generates chlamydia trachomatis specificity
Glycogen, glycogen are constantly accumulated in the cell, are dissolved in cell surface, and under strongly acidic conditions, it is derivative that glycogen dehydration generates furfural
With 2- methylphenol derivative color reaction occurs for object (furfural and hydroxymethylfurfural etc.), on the contrary if aobvious red, is positive
It is then feminine gender；
There are chlamydia trachomatis glycogens for vaginal fluid, in acid condition, phenol formaldehyde condensation reaction occur, ultimately generates network
Object is closed, proportional in color depth and chlamydia trachomatis glycogen content:
(1) it when not containing chlamydia trachomatis glycogen in secretion, does not develop the color or displaing yellow (labeled as "-") indicates yin
Property, instruction vagina state is normal.
(2) when secretion contains chlamydia trachomatis glycogen, show light red, red or aubergine (labeled as " ± " or
"+"), indicating may chlamydia trachomatis infection.
A kind of portable measuring method for measuring of chlamydia trachomatis glycogen test strip of the invention are as follows:
Diluted sample is taken, a drop sample is added dropwise in test strips, in 37 DEG C of 8~10min of warm bath, additional drop colour developing
Liquid, the color change of observable chlamydia trachomatis glycogen test strip, test strips from it is colourless or it is light yellow become light red,
Red or aubergine (different substrate colour developings exist different), that is, be determined as that chlamydia trachomatis glycogen is positive.
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
The preparation of 1 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
100.00mg/mL phenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: the aqueous solution of 100.00mL, 70% dilute sulfuric acid；
20.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
It is as follows that soak drying machine automates drying process:
1, soak drying machine is opened by starting procedure, the drying temperature according to set by embodiment, chart drive speed, to temperature
Degree reaches set temperature, and each dry room temperature reaches predetermined temperature and just can be carried out immersion liquid；
2, the filter paper for using immersion liquid is hung, and immersion liquid slot installs；
3, prepared medical fluid is poured into dipping tank, liquid volume should be at the 1/2 of dipping tank, then by remaining medical fluid
It pours into holding bottle, the speed of regulating liquid medicine injection will contain medicinal cupping sealing；
4, paper feed switch is opened, filter paper is soaked into the liquid, record medical fluid position height with pressure roller；
5, immersion liquid drying course pays attention to observing liquid dose, and dry room temperature meets case study on implementation requirement；
6, the filter paper of immersion liquid is all walked out from hothouse, closes paper feed switch, filter paper is carried out mark, is put into equipped with drying
In the valve bag of agent and it is put into drier stand-by.
The preparation of 2 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM acetic acid-sodium acetate buffer solution；
100.00mg/mL phenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 60% dilute sulfuric acid aqueous solution；
30.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 3 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution；
100.00mg/mL phenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 50% dilute sulfuric acid aqueous solution；
40.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 4 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-potassium phosphate buffer；
100.00mg/mL phenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 40% dilute sulfuric acid aqueous solution；
50.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 5 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM potassium dihydrogen phosphate-sodium hydrate buffer solution；
100.00mg/mL phenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 70% dilute sulfuric acid aqueous solution；
50.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 6 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM barbital sodium-hydrochloride buffer；
100.00mg/mL phenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 30% dilute sulfuric acid aqueous solution；
60.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 7 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
150.00mg/mL 2- methylphenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 20% dilute sulfuric acid aqueous solution；
70.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 8 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
150.00mg/mL4- methylphenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 10% dilute sulfuric acid aqueous solution；
80.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 9 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
150.00mg/mL 5- methylphenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper
Developing solution: 100.00mL, 5% dilute sulfuric acid aqueous solution；
90.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 10 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
120.00mg/mL 7- methylphenol；
10.00mg/mL cystine；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 15% dilute sulfuric acid aqueous solution；
100.00mg/mL sodium thiosulfate；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 11 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
120.00mg/mL 2- chlorophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 25% dilute sulfuric acid aqueous solution；
10.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 12 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
150.00mg/mL4- chlorophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 35% dilute sulfuric acid aqueous solution；
20.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 13 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
150.00mg/mL 5- chlorophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-4000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 45% dilute sulfuric acid aqueous solution；
30.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 14 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
180.00mg/mL 7- chlorophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-6000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 55% dilute sulfuric acid aqueous solution；
40.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 15 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
120.00mg/mL 2- bromophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 65% dilute sulfuric acid aqueous solution；
50.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 16 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
150.00mg/mL4- bromophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-2000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 3.5% dilute sulfuric acid aqueous solution；
60.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 17 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
150.00mg/mL 5- bromophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-4000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 70% dilute sulfuric acid aqueous solution；
70.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
The preparation of 18 chlamydia trachomatis glycogen Test paper of embodiment
Immersion liquid: 300.00mM disodium hydrogen phosphate-citrate buffer solution；
180.00mg/mL 7- bromophenol；
10.00mg/mL BSA；
10.00mg/mLPEG-6000；
PH value is 7.0；
Filter paper type: 31ET type filter paper；
Developing solution: 100.00mL, 50% dilute sulfuric acid aqueous solution；
80.00mg/mL ascorbic acid；
Chlamydia trachomatis glycogen test paper soak drying machine automates drying means:
By 80 DEG C of the main heating setpoint of device temperature after booting, 60 DEG C of secondary heating setpoint reaches 80 DEG C to host heating temperature,
Each dry room temperature starts immersion liquid when reaching 75 DEG C or so；
Soak drying machine walking speed setting: 10.0Hz.
Soak drying machine automates drying process such as embodiment 1.
Test example 1 carries out Performance Evaluation to chlamydia trachomatis glycogen test strip prepared by embodiment 1
1. Appearance Visual
It randomly selects and prepares 10, test paper, test paper appearance property index is checked with twenty-twenty vision, should be met the requirements: 1) surface
Answer smooth, edge impulse- free robustness；2) test block appearance is neat, uniform color.10 times visual observation is qualified.
2. accuracy detects
The test paper for taking preparation detects the reference solution of each concentration level of detection project, and each concentration level repeats
Measurement 5 times, positive reference solution is there is not allowed that negative findings, and negative reference solution is there is not allowed that positive findings, as a result such as 1 institute of table
Show.
1 accuracy data of table
| Measure number | Negative reference solution | Positive low value | Positive high level |
| Rep1 | It is negative | Weakly positive | It is positive |
| Rep2 | It is negative | Weakly positive | It is positive |
| Rep3 | It is negative | Weakly positive | It is positive |
| Rep4 | It is negative | Weakly positive | It is positive |
| Rep5 | It is negative | Weakly positive | It is positive |
3. repeatability detection
Test strips 10 for randomly selecting preparation detect first non-negative magnitude, and all testing results are not
Feminine gender, first non-negative magnitude should be able to be detected accurately, and the results are shown in Table 2.
2 detection limit data of table
| Measure number | Positive low value |
| Rep1 | Weakly positive |
| Rep2 | Weakly positive |
| Rep3 | Weakly positive |
| Rep4 | Weakly positive |
| Rep5 | Weakly positive |
| Rep6 | Weakly positive |
| Rep7 | Weakly positive |
| Rep8 | Weakly positive |
| Rep9 | Weakly positive |
| Rep10 | Weakly positive |
Test example 1 carries out Performance Evaluation to chlamydia trachomatis glycogen test strip prepared by embodiment 5
1. Appearance Visual
It randomly selects and prepares 10, test paper, test paper appearance property index is checked with twenty-twenty vision, should be met the requirements: 1) surface
Answer smooth, edge impulse- free robustness；2) test block appearance is neat, uniform color.10 times visual observation is qualified.
2. accuracy detects
The test paper for taking preparation detects the reference solution of each concentration level of detection project, and each concentration level repeats
Measurement 5 times, positive reference solution is there is not allowed that negative findings, and negative reference solution is there is not allowed that positive findings, as a result such as 3 institute of table
Show.
3 accuracy data of table
| Measure number | Negative reference solution | Positive low value | Positive high level |
| Rep1 | It is negative | Weakly positive | It is positive |
| Rep2 | It is negative | Weakly positive | It is positive |
| Rep3 | It is negative | Weakly positive | It is positive |
| Rep4 | It is negative | Weakly positive | It is positive |
| Rep5 | It is negative | Weakly positive | It is positive |
3. repeatability detection
Test strips 10 for randomly selecting preparation detect first non-negative magnitude, and all testing results are not
Feminine gender, first non-negative magnitude should be able to be detected accurately, and the results are shown in Table 4.
4 detection limit data of table
| Measure number | Positive low value |
| Rep1 | Weakly positive |
| Rep2 | Weakly positive |
| Rep3 | Weakly positive |
| Rep4 | Weakly positive |
| Rep5 | Weakly positive |
| Rep6 | Weakly positive |
| Rep7 | Weakly positive |
| Rep8 | Weakly positive |
| Rep9 | Weakly positive |
| Rep10 | Weakly positive |
Test example 1 carries out Performance Evaluation to chlamydia trachomatis glycogen test strip prepared by embodiment 8
1. Appearance Visual
It randomly selects and prepares 10, test paper, test paper appearance property index is checked with twenty-twenty vision, should be met the requirements: 1) surface
Answer smooth, edge impulse- free robustness；2) test block appearance is neat, uniform color.10 times visual observation is qualified.
2. accuracy detects
The test paper for taking preparation detects the reference solution of each concentration level of detection project, and each concentration level repeats
Measurement 5 times, positive reference solution is there is not allowed that negative findings, and negative reference solution is there is not allowed that positive findings, as a result such as 5 institute of table
Show.
5 accuracy data of table
| Measure number | Negative reference solution | Positive low value | Positive high level |
| Rep1 | It is negative | Weakly positive | It is positive |
| Rep2 | It is negative | Weakly positive | It is positive |
| Rep3 | It is negative | Weakly positive | It is positive |
| Rep4 | It is negative | Weakly positive | It is positive |
| Rep5 | It is negative | Weakly positive | It is positive |
3. repeatability detection
Test strips 10 for randomly selecting preparation detect first non-negative magnitude, and all testing results are not
Feminine gender, first non-negative magnitude should be able to be detected accurately, and the results are shown in Table 6.
6 detection limit data of table
Test example 1 carries out Performance Evaluation to chlamydia trachomatis glycogen test strip prepared by embodiment 10
1. Appearance Visual
It randomly selects and prepares 10, test paper, test paper appearance property index is checked with twenty-twenty vision, should be met the requirements: 1) surface
Answer smooth, edge impulse- free robustness；2) test block appearance is neat, uniform color.10 times visual observation is qualified.
2. accuracy detects
The test paper for taking preparation detects the reference solution of each concentration level of detection project, and each concentration level repeats
Measurement 5 times, positive reference solution is there is not allowed that negative findings, and negative reference solution is there is not allowed that positive findings, as a result such as 7 institute of table
Show.
7 accuracy data of table
| Measure number | Negative reference solution | Positive low value | Positive high level |
| Rep1 | It is negative | Weakly positive | It is positive |
| Rep2 | It is negative | Weakly positive | It is positive |
| Rep3 | It is negative | Weakly positive | It is positive |
| Rep4 | It is negative | Weakly positive | It is positive |
| Rep5 | It is negative | Weakly positive | It is positive |
3. repeatability detection
Test strips 10 for randomly selecting preparation detect first non-negative magnitude, and all testing results are not
Feminine gender, first non-negative magnitude should be able to be detected accurately, and the results are shown in Table 8.
8 detection limit data of table
Test example 1 carries out Performance Evaluation to chlamydia trachomatis glycogen test strip prepared by embodiment 13
1. Appearance Visual
It randomly selects and prepares 10, test paper, test paper appearance property index is checked with twenty-twenty vision, should be met the requirements: 1) surface
Answer smooth, edge impulse- free robustness；2) test block appearance is neat, uniform color.10 times visual observation is qualified.
2. accuracy detects
The test paper for taking preparation detects the reference solution of each concentration level of detection project, and each concentration level repeats
Measurement 5 times, positive reference solution is there is not allowed that negative findings, and negative reference solution is there is not allowed that positive findings, as a result such as 9 institute of table
Show.
9 accuracy data of table
| Measure number | Negative reference solution | Positive low value | Positive high level |
| Rep1 | It is negative | Weakly positive | It is positive |
| Rep2 | It is negative | Weakly positive | It is positive |
| Rep3 | It is negative | Weakly positive | It is positive |
| Rep4 | It is negative | Weakly positive | It is positive |
| Rep5 | It is negative | Weakly positive | It is positive |
3. repeatability detection
Test strips 10 for randomly selecting preparation detect first non-negative magnitude, and all testing results are not
Feminine gender, first non-negative magnitude should be able to be detected accurately, and the results are shown in Table 10.
10 detection limit data of table
Test example 1 carries out Performance Evaluation to chlamydia trachomatis glycogen test strip prepared by embodiment 15
1. Appearance Visual
It randomly selects and prepares 10, test paper, test paper appearance property index is checked with twenty-twenty vision, should be met the requirements: 1) surface
Answer smooth, edge impulse- free robustness；2) test block appearance is neat, uniform color.10 times visual observation is qualified.
2. accuracy detects
The test paper for taking preparation detects the reference solution of each concentration level of detection project, and each concentration level repeats
Measurement 5 times, positive reference solution is there is not allowed that negative findings, and negative reference solution is there is not allowed that positive findings, as a result such as 2 institute of table
Show.
11 accuracy data of table
| Measure number | Negative reference solution | Positive low value | Positive high level |
| Rep1 | It is negative | Weakly positive | It is positive |
| Rep2 | It is negative | Weakly positive | It is positive |
| Rep3 | It is negative | Weakly positive | It is positive |
| Rep4 | It is negative | Weakly positive | It is positive |
| Rep5 | It is negative | Weakly positive | It is positive |
3. repeatability detection
Test strips 10 for randomly selecting preparation detect first non-negative magnitude, and all testing results are not
Feminine gender, first non-negative magnitude should be able to be detected accurately, as a result as shown in table 12.
12 detection limit data of table
| Measure number | Positive low value |
| Rep1 | Weakly positive |
| Rep2 | Weakly positive |
| Rep3 | Weakly positive |
| Rep4 | Weakly positive |
| Rep5 | Weakly positive |
| Rep6 | Weakly positive |
| Rep7 | Weakly positive |
| Rep8 | Weakly positive |
| Rep9 | Weakly positive |
| Rep10 | Weakly positive |
Test example 1 carries out Performance Evaluation to chlamydia trachomatis glycogen test strip prepared by embodiment 18
1. Appearance Visual
It randomly selects and prepares 10, test paper, test paper appearance property index is checked with twenty-twenty vision, should be met the requirements: 1) surface
Answer smooth, edge impulse- free robustness；2) test block appearance is neat, uniform color.10 times visual observation is qualified.
2. accuracy detects
The test paper for taking preparation detects the reference solution of each concentration level of detection project, and each concentration level repeats
Measurement 5 times, positive reference solution is there is not allowed that negative findings, and negative reference solution is there is not allowed that positive findings, as a result such as table 13
It is shown.
13 accuracy data of table
3. repeatability detection
Test strips 10 for randomly selecting preparation detect first non-negative magnitude, and all testing results are not
Feminine gender, first non-negative magnitude should be able to be detected accurately, as a result as shown in table 14.
14 detection limit data of table
| Measure number | Positive low value |
| Rep1 | Weakly positive |
| Rep2 | Weakly positive |
| Rep3 | Weakly positive |
| Rep4 | Weakly positive |
| Rep5 | Weakly positive |
| Rep6 | Weakly positive |
| Rep7 | Weakly positive |
| Rep8 | Weakly positive |
| Rep9 | Weakly positive |
| Rep10 | Weakly positive |
It is shown by the testing result of above-mentioned test example, heretofore described test paper, appearance surfaces answer smooth, test block
Appearance is neat, uniform color, has observability；Detect positive reference solution, the accurate zero deflection of negative reference solution result；Weight
Renaturation detection has homogeneity and consistency, and all Testing index test results meet product requirement.Product of the present invention has
Detection speed is fast, can estimate the good advantage of view, high sensitivity, stability of testing, and dry chemical chemical method can sufficiently make up market production
Product blank, it is easy to operate compared with similar products, it is suitable for universal.
The invention discloses a kind of chlamydia trachomatis glycogen detection reagent, chlamydia trachomatis glycogen test strip and its systems
Preparation Method, those skilled in the art can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.Product of the invention is described by preferred embodiment, related personnel obviously can not depart from the content of present invention,
Product as described herein is modified in spirit and scope or appropriate changes and combinations, carrys out implementation and application the technology of the present invention.
Claims (10)
1. a kind of chlamydia trachomatis glycogen detection reagent characterized by comprising substrate, buffer, developing solution, auxiliary reagent；
The substrate is phenol, phenol derivatives, naphthylamines, naphthylamine derivative or anthracyclinone derivatives；
The developing solution includes principal component and inoxidizability ingredient, and the principal component is water-soluble less than or equal to 70% dilute sulfuric acid
Liquid, the inoxidizability ingredient are sodium thiosulfate, ascorbic acid, thiocarbamide, hydrogen peroxide or urea peroxide；
The auxiliary reagent includes stabilizer, reducing agent and dispersing agent.
2. a kind of chlamydia trachomatis glycogen detection reagent according to claim 1, which is characterized in that the concentration of the substrate
For 1~200mg/mL.
3. a kind of chlamydia trachomatis glycogen detection reagent according to claim 1, which is characterized in that the substrate is selected from benzene
Phenol, 2- methylphenol, 4- methylphenol, 2- chlorophenol, 4- chlorophenol, 2- bromophenol, 4- bromophenol, anthrone, 2- methyl anthrone,
4- methyl anthrone, 2- chrloroanthracene ketone, 4- chrloroanthracene ketone, 2- bromine anthrone, 4- bromine anthrone, 5- methyl anthrone, 7- methyl anthrone, 5- chrloroanthracene
One of ketone, 5- bromine anthrone.
4. a kind of chlamydia trachomatis glycogen detection reagent according to claim 1, which is characterized in that the pH of the buffer
It is 5~10, concentration is 100~500mM, is selected from disodium hydrogen phosphate-citrate buffer solution, acetic acid-sodium acetate buffer solution, phosphoric acid hydrogen
Disodium-phosphate sodium dihydrogen buffer solution, disodium hydrogen phosphate-potassium phosphate buffer, potassium dihydrogen phosphate-sodium hydrate buffer solution,
One of barbital sodium-hydrochloride buffer is a variety of.
5. a kind of chlamydia trachomatis glycogen detection reagent according to claim 1, which is characterized in that in the developing solution,
The volume of principal component is 100mL, and the concentration of inoxidizability ingredient is 1~100mg/mL.
6. a kind of chlamydia trachomatis glycogen detection reagent according to claim 1, which is characterized in that the stabilizer it is dense
Degree is 5~20mg/mL, is selected from one of cystine, leucine, bovine serum albumin(BSA), L-cysteine or a variety of.
7. a kind of chlamydia trachomatis glycogen detection reagent according to claim 1, which is characterized in that the reducing agent it is dense
Degree are as follows: 1.00~100.0mg/mL, selected from one of sodium thiosulfate, ascorbic acid, thiocarbamide, hydrogen peroxide or urea peroxide
Or it is a variety of.
8. a kind of chlamydia trachomatis glycogen detection reagent according to claim 1, which is characterized in that the dispersing agent it is dense
Degree is 1~20mg/mL, selected from one of PVP-K90, PEG-2000, PEG-4000, PEG-6000 or a variety of.
9. the chlamydia trachomatis containing a kind of chlamydia trachomatis glycogen detection reagent described in any one of claim 1 to 8
Glycogen test strip.
10. the method for preparing a kind of chlamydia trachomatis glycogen test strip as claimed in claim 9, which is characterized in that including
Following steps:
Step 1: preparating liquid
Buffer and auxiliary reagent are uniformly mixed, substrate is added and is configured to medical fluid；
Step 2: dry
It is dried using soak drying machine automation drying means；
The setting of soak drying machine temperature is as follows:
Main heating temperature is set as 75~85 DEG C after booting, secondary heating temperature is set as 55~75 DEG C, reaches to main heating temperature
To 75~85 DEG C, filter paper is soaked into the liquid beginning immersion liquid when each dry room temperature >=75 DEG C；
The setting of soak drying machine walking speed is as follows:
6.0~12.0Hz.
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| CN111257254A (en) * | 2020-03-19 | 2020-06-09 | 中国水产科学研究院黄海水产研究所 | Method for measuring glycogen content in oyster tissue |
| CN111665241A (en) * | 2020-06-12 | 2020-09-15 | 苏州良辰生物仪器试剂有限公司 | Tyrosine detection test strip and preparation method and application thereof |
| CN113376150A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Urine microalbumin dry chemical detection test paper and preparation method thereof |
| CN113567665A (en) * | 2021-08-16 | 2021-10-29 | 固安林科特生物工程有限公司 | Lysate for detecting chlamydia trachomatis antigen and detection method |
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