CN110302850A - A kind of centralized liquid release device - Google Patents
A kind of centralized liquid release device Download PDFInfo
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- CN110302850A CN110302850A CN201910496794.8A CN201910496794A CN110302850A CN 110302850 A CN110302850 A CN 110302850A CN 201910496794 A CN201910496794 A CN 201910496794A CN 110302850 A CN110302850 A CN 110302850A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention belongs to micro fluidic chip technical fields, a kind of centralized liquid release device is disclosed, including centrifugal chip, the centrifugal chip has rotation center, at least one set of relieving mechanism is provided on the centrifugal chip, the relieving mechanism includes liquid inlet, vesica position-limit mechanism, pressing mechanism, liquid storage vesica, the liquid inlet is connected to the runner in the centrifugal chip, the vesica position-limit mechanism is fixed at side of the liquid inlet far from the rotation center, the pressing mechanism is slidably arranged in the liquid inlet close to the side of the rotation center, the liquid storage vesica is arranged between the vesica position-limit mechanism and the pressing mechanism.A kind of centralized liquid release device of the invention can be realized the release of liquid by simple means, and be able to achieve the orderly release of multiple groups liquid, without using complicated mechanical structure.
Description
Technical field
The present invention relates to micro fluidic chip technical field more particularly to a kind of centralized liquid release devices.
Background technique
Centrifugal microfluidic control chip completes complicated fluid reaction using the manipulation of single centrifuge, and is suitble to high-throughput detection,
It is widely used in biochemical analysis and in-vitro diagnosis field.In order to improve the portability of micro-fluidic chip testing product and controllable
Property, usually require that the pre-buried required liquid reagent on chip, and complete the controlled release of mentioned reagent.The controllable sequence of reagent
Release helps to complete complex biochemical reaction on chip.Current centrifugal chip lacks liquid release flexibly, quickly, inexpensive
Means.Moreover, on chip the pre-buried influence chip of liquid reagent stability, influence the activity of pre-buried freeze-dried reagent on chip,
It is at high cost and complicated for operation.Realize that the storage and release of plurality of reagents on one chip often require to use complicated machine
Tool structure, or even need to increase additional ancillary equipment.Biochemical reaction generally involves multiple fluid, and multiple fluid is needed to have
There is the reaction of certain sequence and time interval, therefore conventional microfluidic control chip is related to multiple control valves for fluids.
Although there has been proposed various schemes at present, such as application No. is the Chinese invention patent of CN201410750822.1,
Disclose a kind of micro-fluidic chip liquid reagent method for releasing based on centrifugal action, the program can solve certain problem but still
Fluid storage is had the following deficiencies: in bag, the sealing of bag is more demanding, and the relative position of bag and chip discharges it
Stability influence is larger, in addition, the usual area of micro-fluidic chip is little, the area of bag is larger in the patent, is unfavorable for chip
Other function unit integrates.
For another example application No. is the Chinese invention patent applications of CN201410631614.X, disclose a kind of in-vitro diagnosis test
Card, the program can solve certain problem but still have the following deficiencies: the liquid by fashion of extrusion release storage in the chips,
Although which controllability is good, increases moving component, increase the complexity of equipment.
Based on the above situation, we it is necessary to design a kind of centralized liquid release device for being able to solve the above problem.
Summary of the invention
It is an object of the invention to: a kind of centralized liquid release device is provided, can realize liquid by simple means
Release, and it is able to achieve the orderly release of multiple groups liquid, without using complicated mechanical structure.
To achieve this purpose, the present invention adopts the following technical scheme:
A kind of centralized liquid release device, including centrifugal chip, the centrifugal chip has rotation center, described
At least one set of relieving mechanism is provided on centrifugal chip, the relieving mechanism includes liquid inlet, vesica position-limit mechanism, squeezes
Mechanism, liquid storage vesica, the liquid inlet are connected to the runner in the centrifugal chip, and the vesica position-limit mechanism fixation is set
It sets in side of the liquid inlet far from the rotation center, it is close that the pressing mechanism is slidably arranged in the liquid inlet
The side of the rotation center, the liquid storage vesica are arranged between the vesica position-limit mechanism and the pressing mechanism.
Specifically, centrifugal control device drives the centrifugal chip to rotate around the rotation center, acts in centrifugal force
Under, the pressing mechanism is slided towards the vesica position-limit mechanism, and the pressing mechanism has pre-packaged described in target liq
Liquid storage vesica is extruded on the vesica position-limit mechanism, and the liquid storage vesica is located above the liquid inlet at this time, is being centrifuged
When power is sufficiently large, the liquid storage capsules rupture, the liquid release in the liquid storage vesica, and institute is entered by the liquid inlet
It states in centrifugal chip.
Preferably, the centrifugal chip is in disk form, the rotation center be disk the center of circle, the pressing mechanism,
The liquid storage vesica and the vesica position-limit mechanism are successively arranged outward along the radial direction of the centrifugal chip.
Preferably, the shape of the vesica position-limit mechanism, the pressing mechanism and the liquid storage vesica matches, it is ensured that institute
Show that liquid storage vesica and shown pressing mechanism will not occur obviously to be displaced under high-speed rotation, and ensures the liquid storage vesica energy quilt
It squeezes, the liquid in being encapsulated in can be released completely completely.
Preferably, the pressing mechanism is crushing block.
Preferably, the centrifugal control device is centrifuge, and the centrifuge is fixedly connected with the rotation center.
As a kind of perferred technical scheme, puncturing towards the liquid storage vesica is provided on the vesica position-limit mechanism
Structure.
Specifically, the puncture structure has sharp tip, is extruded into the vesica limit in the liquid storage vesica
When in mechanism, the puncture structure can be more easier to puncture the liquid storage vesica.The quantity of the puncture structure is one or more
It is a.
As a kind of perferred technical scheme, the puncture structure is close to the bottom of the liquid storage vesica.
Specifically, the puncture structure makes liquid storage vesica bottom in rupture close to the bottom of the liquid storage vesica
It is preferentially punctured, is conducive to liquid in the liquid storage vesica and discharges completely.
As a kind of perferred technical scheme, fixing groove, the vesica position-limit mechanism are provided on the centrifugal chip
Lower end is removably disposed in fixing groove.
Specifically, vesica position-limit mechanism lower end is provided with the plug division matched with the fixing groove, the grafting
Portion's geomery is matched with the fixing groove geomery, and the plug division and the fixing groove form plug cooperation.It is described
Vesica position-limit mechanism is detachably connected with the centrifugal chip, is conveniently retained separately, packs, is transported, and is assembled when use,
Increase the flexibility of device.
As a kind of perferred technical scheme, vesica position-limit mechanism lower end covers a part of the liquid inlet.
Specifically, the vesica position-limit mechanism is located at side of the liquid inlet far from the rotation center, the liquid
The opening of body entrance is covered far from a part of the rotation center by vesica position-limit mechanism lower end, makes to limit along the vesica
The liquid that position mechanism flows down can flow directly into the liquid inlet, prevent liquid entry into and cause to waste in fixing groove, it is ensured that
The liquid of release all passes through the liquid inlet and enters in the centrifugal chip.
As a kind of perferred technical scheme, the side of the vesica position-limit mechanism towards the liquid storage vesica is provided with U
The shape of type opening, the liquid storage vesica and/or the pressing mechanism is matched with the U-shaped opening.
Specifically, the liquid storage vesica and the pressing mechanism can be accommodated in the inner by the U-shaped opening, the U-shaped opening
The liquid storage vesica and the pressing mechanism can be limited, make the liquid storage vesica and the pressing mechanism along the vesica
Position-limit mechanism sliding, it is ensured that the liquid storage vesica and the pressing mechanism will not be when shown centrifugal chip high speed rotate
Obvious displacement.And ensure that the liquid storage vesica can be squeezed completely, the liquid in being encapsulated in can be released completely.
Preferably, the shape of the liquid storage vesica and the pressing mechanism is matched with the U-shaped opening.
As a kind of perferred technical scheme, vesica position-limit mechanism upper end is provided with limit plate.
Specifically, the limit plate is in the upper end of the U-shaped vesica position-limit mechanism, it is ensured that the liquid storage vesica and institute
It states crushing block and does not deviate by the entire vesica position-limit mechanism under the action of the centrifugal.
As a kind of perferred technical scheme, the side of the vesica position-limit mechanism towards the liquid storage vesica is provided with thin
Water layer.
Specifically, the inner wall of the vesica position-limit mechanism towards the side of the liquid storage vesica is formed as hydrophobic treatment thin
Water layer can be reduced absorption of the vesica position-limit mechanism to liquid reagent, liquid enable all smoothly to flow down.
As a kind of perferred technical scheme, the inner wall of the vesica position-limit mechanism towards the side of the liquid storage vesica is
Incline structure, the upper end of the incline structure is than lower end closer to the rotation center.
Specifically, the inner wall has certain tilt angle, and under the influence of centrifugal force, liquid is easier to enter institute
It states in liquid inlet.
As a kind of perferred technical scheme, it is arranged on the vesica position-limit mechanism and/or the pressing mechanism and is magnetic
Mechanism.
Specifically, it is respectively arranged with the magnet to attract each other on the vesica position-limit mechanism and the pressing mechanism, alternatively,
One of them is provided with magnet for the vesica position-limit mechanism, the pressing mechanism, another be provided with by can by magnet adsorb
Structure made of material.It is more advantageous to the fixation of the pressing mechanism and the vesica position-limit mechanism in this way, and to the storage
The extruding of liquid capsule bubble and the release of liquid.
The invention has the benefit that providing a kind of centralized liquid release device, liquid is realized by simple means
Release, and it is able to achieve the orderly release of multiple groups liquid, without using complicated mechanical structure；
Liquid storage vesica does not need to be embedded on chip in advance, saves the space of chip, is conducive to a variety of on one chip
The storage of reagent and orderly release；
The direct storage for avoiding liquid on chip, enhances the stability of chip, especially for having freeze-dried reagent on chip
In the case of, liquid storage vesica is individually present, and avoids the interference to freeze-dried reagent.
Reagent and chip separation, reagent type and volume can arbitrarily change, and flexibility is good；
Under the influence of centrifugal force by pressing mechanism, the vesica position restrainer on liquid storage vesica and centrifugal chip is squeezed
Structure crushes liquid storage vesica, discharges liquid；
Liquid storage vesica and pressing mechanism assembly type exist, and flexibility is preferable, avoid the use of complicated mechanical structure, keep away
The use of complicated control valve for fluids is exempted from；
Pressing mechanism and vesica position-limit mechanism are reusable, and cost is relatively low.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the embodiment of the present invention one；
Fig. 2 is the status diagram in the use of the embodiment of the present invention one；
Fig. 3 is the status diagram after the use of the embodiment of the present invention one；
Fig. 4 is the structural schematic diagram of vesica position-limit mechanism and fixing groove in the embodiment of the present invention one；
Fig. 5 is the cross-sectional view of vesica position-limit mechanism and pressing mechanism in the embodiment of the present invention one；
Fig. 6 is the vesica position-limit mechanism of another shape and the cross-sectional view of pressing mechanism in the embodiment of the present invention one；
Fig. 7 is structural schematic diagram when vesica position restrainer inner wall is incline structure in the embodiment of the present invention one；
Fig. 8 is the structural schematic diagram of the embodiment of the present invention two.
Wherein, Fig. 1 is into Fig. 7, centrifugal chip 1, rotation center 2, liquid inlet 3, vesica position-limit mechanism 4, extruder
Structure 5, liquid storage vesica 6, puncture structure 7, fixing groove 8, U-shaped opening 9, limit plate 10, plug division 41.
In Fig. 8, relieving mechanism (100,200,300,400), vesica position-limit mechanism (104,204,304,404).
Specific embodiment
, there are further understanding and understanding in the effect of to make to structure feature of the invention and being reached, to preferable
Examples and drawings cooperation detailed description, is described as follows:
Embodiment one
A kind of centralized liquid release device, as shown in Figure 1, including centrifugal chip 1, centrifugal chip 1 has rotation
Center 2 is provided at least one set of relieving mechanism on centrifugal chip 1 and (in Fig. 1 to Fig. 4, Fig. 7 for ease of description, only draws one
Group relieving mechanism), relieving mechanism includes liquid inlet 3, vesica position-limit mechanism 4, pressing mechanism 5, liquid storage vesica 6, liquid inlet 3
It is connected to the runner in centrifugal chip 1, vesica position-limit mechanism 4 is fixed at one of liquid inlet 3 far from rotation center 2
Side, pressing mechanism 5 are slidably arranged in liquid inlet 3 close to the side of rotation center 2, and liquid storage vesica 6 is arranged in vesica position restrainer
Between structure 4 and pressing mechanism 5.
Centrifugal chip 1 is in disk form, and rotation center 2 is the center of circle of disk, and centrifuge (not shown) and rotation center 2 are solid
It is fixed to connect and centrifugal chip 1 be driven to rotate, the revolving speed of centrifugal chip 1 can control by certain control program, add
The adjusting slowed down and turned to, this is conventional technical means in the art, and details are not described herein.Pressing mechanism 5, liquid storage vesica 6 and capsule
The radial direction for steeping position-limit mechanism 4 along centrifugal chip 1 successively arranges that sufficiently large in the revolving speed of centrifugal chip 1, centrifugal force is sufficient outward
When enough big, liquid storage vesica 6 can squeeze rupture between pressing mechanism 5 and vesica position-limit mechanism 4, and the liquid in liquid storage vesica 6 is released
It puts, and is entered in the fluid channel in centrifugal chip 1 by liquid inlet 3.
The material of centrifugal chip 1 includes glass, silicon wafer or common polymer material.Polymer material includes
Dimethyl silicone polymer (PDMS), polyurethane, epoxy resin, polymethyl methacrylate (PMMA), polycarbonate (PC), cyclenes
Hydrocarbon copolymer (COC), polystyrene (PS), polyethylene (PE), fluoroplastics.The material of centrifugal chip 1 can make above-mentioned material
One of or it is several.The processing method of centrifugal chip 1 according to material and structure, can choose photoetching, numerical control, casting,
One of distinct methods such as injection molding, laser engraving, plasma etching, wet etching are several.
It include target liq in liquid storage vesica 6, (common volume 2-200 μ in field is arranged according to requirement of experiment for volume
L), material can be soft polymer or composite material common in field, such as aluminum composite material.Liquid storage vesica 6 is processed
Process belongs to conventional technical means in the art, and details are not described herein.Liquid storage vesica 6 is required to be placed in vesica position-limit mechanism 4
Between pressing mechanism 5, i.e., 6 shape of liquid storage vesica can be according to processing conditions and vesica position-limit mechanism 4 and pressing mechanism 5
Shape is configured.
The material of vesica position-limit mechanism 4 can be the common material such as polymer, metal, glass.Processing method can be number
The common approach such as control processing, laser processing or injection molding.Pressing mechanism 5 is crushing block, and crushing block is consolidating for hard rigid material
Body (metal, polymer, glass etc.).
In this present embodiment, as shown in Figure 1, being provided with the puncture structure 7 towards liquid storage vesica 6 on vesica position-limit mechanism 4.
As shown in Figure 2 to Figure 3, puncture structure 7 has sharp tip, when liquid storage vesica 6 is extruded on vesica position-limit mechanism 4,
Puncture structure 7 can be more easier to puncture liquid storage vesica 6, release the liquid in liquid storage vesica 6.The quantity of puncture structure 7
For one or more.6 side polymer material of liquid storage vesica can be thinned in 6 process of liquid storage vesica if necessary,
Relatively thin 6 vesica side of liquid storage vesica and puncture structure 7 are contacted when installation, are more advantageous to liquid in liquid storage vesica 6 in this way
Release.
In this present embodiment, as shown in Figure 1, puncture structure 7 is rupturing liquid storage vesica 6 close to the bottom of liquid storage vesica 6
When bottom be preferentially punctured, be conducive to liquid in liquid storage vesica 6 and discharge completely.In other embodiments, puncture structure 7 can position
In any position of 4 inner wall of vesica position-limit mechanism, it is only necessary to guarantee pressing mechanism 5 under the action of the centrifugal force, squeeze 6 He of liquid storage vesica
Puncture structure 7 contacts.
In this present embodiment, as shown in figure 4, being provided with fixing groove 8 on centrifugal chip 1,4 lower end of vesica position-limit mechanism can
Releasably it is arranged in fixing groove 8.4 lower end of vesica position-limit mechanism is provided with the plug division 41 matched with fixing groove 8, plug division
41 geomeries are matched with the geomery of fixing groove 8, and plug division 41 and fixing groove 8 form plug cooperation, centrifugal chip 1
When rotation, vesica position-limit mechanism 4 is securely inserted into fixing groove 8 by plug division 41.
In this present embodiment, a part of 4 lower end Covering Liguid entrance 3 of vesica position-limit mechanism.As shown in figure 4, plug division
41 width is W1, and the width of fixing groove 8 is W2, W1 > W2, after the inserting of vesica position-limit mechanism 4 is into fixing groove 8, it is ensured that capsule
Steep 4 lower end of position-limit mechanism can only Covering Liguid entrance 3 a part, and liquid inlet 3 cannot all be covered, make the liquid of release
Body all passes through liquid inlet 3 and enters in centrifugal chip 1 without remaining in fixing groove 8.
In this present embodiment, as shown in Figure 5 to Figure 6, the side of vesica position-limit mechanism 4 towards liquid storage vesica 6 is provided with U-shaped
The shape of opening 9, liquid storage vesica 6 and pressing mechanism 5 is matched with U-shaped opening 9.U-shaped opening shown in Fig. 5 is opened for arc
Mouthful, the pressed end of pressing mechanism 5 is arc-shaped, and U-shaped opening shown in Fig. 6 is frame shape opening, and the pressed end of pressing mechanism 5 is in frame
Shape.Liquid storage vesica 6 and pressing mechanism 5 can be accommodated in the inner by U-shaped opening 9, and U-shaped opening 9 can be to liquid storage vesica 6 and pressing mechanism 5
It is limited, liquid storage vesica 6 and pressing mechanism 5 are slided along vesica position-limit mechanism 4 without sending out when centrifugal 1 high-speed rotation of chip
Raw obvious displacement.And ensure that liquid storage vesica 6 can be squeezed completely, and the liquid in being encapsulated in is released completely.It is worth saying
Bright, U-shaped opening is not limited to diagrammatically shown form, as long as can ensure that liquid storage vesica 6 and pressing mechanism 5 in centrifugal chip 1
It will not occur obviously to be displaced when high-speed rotation.
In this present embodiment, as shown in Figure 1,4 upper end of vesica position-limit mechanism is provided with limit plate 10, the fixation of limit plate 10 is set
It sets in 4 upper end of position-limit mechanism, can prevent liquid storage vesica 6 and pressing mechanism 5 from limiting in centrifugal 1 high-speed rotation of chip from vesica
It is skidded off above mechanism 4.Limit plate 10 is an integral molding structure with vesica position-limit mechanism 4 in the present embodiment.
In this present embodiment, the inner wall of vesica position-limit mechanism 4 towards the side of liquid storage vesica 6 is provided with hydrophobic layer.
In this present embodiment, as shown in fig. 7, the inner wall of vesica position-limit mechanism 4 towards the side of liquid storage vesica 6 is bevelled junction
Structure, the upper end of incline structure than lower end closer to rotation center 2, side shape of the pressing mechanism towards liquid storage vesica 6 at this time
For incline structure.
In this present embodiment, magnetic mechanism is provided on vesica position-limit mechanism 4 and/or pressing mechanism 5.Vesica position-limit mechanism
4 and pressing mechanism 5 on be respectively arranged with the magnet to attract each other, alternatively, vesica position-limit mechanism 4, pressing mechanism 5 one of them set
It is equipped with magnet, another is provided with the structure made of the material that can be adsorbed by magnet.It is more advantageous to pressing mechanism 5 and capsule in this way
The fixation of position-limit mechanism 4 is steeped, and to the extruding of liquid storage vesica 6 and the release of liquid.
When biochemical reaction is related to multiple fluid, and when needing multiple fluid reaction with time interval in certain sequence,
Multiple fluid inlets 3 can be set on centrifugal chip 1 in the present invention, and the multiple vesica position-limit mechanisms 4 of corresponding setting squeeze
Mechanism 5, liquid storage vesica 6.
The present invention can realize the orderly release of fluid by Multiple factors, and principal element includes:
Distance of the relieving mechanism apart from rotation center 2, under equal conditions, more early release remoter apart from rotation center 2；
The weight of pressing mechanism 5, under equal conditions, pressing mechanism 5 is heavier, more early release；
The factor of other emphasis includes the material (type and thickness of packaging material) of liquid storage vesica 6, pressing mechanism 5
Shape etc..
The orderly release of multiple groups liquid can be realized by adjusting above-mentioned factor.
Embodiment two
Centralized liquid release device of the invention is described in detail by taking magnetic particle double antibodies sandwich chemiluminescence as an example in the present embodiment
The course of work.
Common reactant system of the magnetic particle double antibodies sandwich chemiluminescence as current in-vitro diagnosis field, is widely used in
Clinical monitoring.Centrifugal chip is widely used in inspection body, base etc. due to the features such as its equipment is small-sized, manipulation is simple.
In order to improve the stability of reagent, the requirement of low temperature in transportational process is reduced, at present usually by antibody etc. in field
Lyophilized form is pre-stored in centrifugal chip.The redissolution of lyophilized antibodies needs the addition of dilution, if dilution be embedded in from
On core type chip, it is most likely that influence the activity of lyophilized antibodies, such as above-mentioned dilution by volatilizing so that lyophilized antibodies are made moist.
The present embodiment is by taking magnetic particle double antibodies sandwich chemiluminescence as an example, as shown in figure 8, antibody and magnetic bead etc. needed for reaction
It is embedded in centrifugal chip 1 by freeze-drying.The present embodiment be provided on centrifugal chip four groups of relieving mechanisms (100,200,
300,400), for the ease of statement, in figure every group of relieving mechanism illustrate only corresponding vesica position-limit mechanism (104,204,304,
404), fluid inlet, pressing mechanism, liquid storage vesica are not shown.Remaining structure is identical with embodiment one, no longer superfluous herein
It states.
The liquid encapsulated in liquid storage vesica (not shown) at four groups of relieving mechanisms (100,200,300,400) is respectively clear
Washing lotion, substrate solution, primary antibody dilution, magnetic bead dilution.
The design of chip runner requires to be designed according to reaction, and non-present invention emphasis, and is the conventional skill of this field
Art means, therefore do not shown in figure.
In implementation process, four groups of relieving mechanisms (100,200,300,400) are assembled on centrifugal chip 1 first.With
The center of discoidal centrifugal chip 1 is the center of circle, and relieving mechanism (100) and relieving mechanism (200) on the same circumference, discharge
Mechanism (300) and relieving mechanism (400) are on the same circumference.The wherein pressing mechanism (not shown) matter at relieving mechanism (300)
Amount is greater than the pressing mechanism (not shown) at relieving mechanism (400), the liquid storage vesica (not shown) material at relieving mechanism (100)
It is thinner compared to the liquid storage vesica (not shown) at relieving mechanism (200).
Test substance is added in the target runner of centrifugal chip 1 first in reaction process.
Since relieving mechanism (300), relieving mechanism (400) are far from the center of circle, compared to relieving mechanism (100), relieving mechanism
(200), in the case of revolving speed steps up, in the liquid storage vesica (not shown) at relieving mechanism (300), relieving mechanism (400)
Liquid discharges first, since the quality of the pressing mechanism (not shown) at relieving mechanism (300) is greater than at relieving mechanism (400)
The quality of pressing mechanism (not shown), under 1 revolving speed of ω, the primary antibody dilution at relieving mechanism (300) discharges first, and primary antibody is answered
It is combined after molten with test substance.
Revolving speed is increased to ω 2, and the magnetic bead dilution release at relieving mechanism (400), magnetic bead redissolves, and participates in above-mentioned reaction,
Form double antibodies sandwich structure.
Revolving speed is increased to ω 3, and relieving mechanism (100), relieving mechanism (200) are close to the center of circle, relieving mechanism (100), release
The liquid in liquid storage vesica (not shown) at mechanism (200) starts to discharge, due to the liquid storage vesica material at relieving mechanism (100)
Material is thinner compared to the liquid storage vesicle material at relieving mechanism (200), and the cleaning solution at relieving mechanism (100) discharges first, needle
The double antibodies sandwich structure of formation is cleaned.
Revolving speed is increased to ω 4, and substrate solution release and double antibodies sandwich structural response at relieving mechanism (200) shine and carries out
Magnetic particle double antibodies sandwich chemiluminescence reaction is completed in detection.
2 > ω of above-mentioned 1 > ω of ω, 3 > ω 4.
The orderly release of liquid, can be by adjusting the position of relieving mechanism, the material and thickness, centrifugation turn of liquid storage vesica
The factors such as the fast, shape of pressing mechanism and quality, the quantity of relieving mechanism and arrangement are realized, will not repeat them here.
The part that the present invention is not directed to is the same as those in the prior art or can be realized by using the prior art.
Finally, it should be noted that in the description of the present invention, technical term "center", "upper", "lower", "left", "right",
"front", "rear", "inner", "outside", "vertical", "horizontal" etc. indicate direction or positional relationship is direction or position based on the figure
Relationship is set, is merely for convenience of describing and understand technical solution of the present invention, rather than the device or member of indication or suggestion meaning
Part must have a particular orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.This
Outside, term " first ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.?
In description of the invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " connected ", " connection "
It shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can be mechanical connect
It connects, is also possible to be electrically connected；It can be directly connected, can also can be in two elements indirectly connected through an intermediary
The connection in portion.For the ordinary skill in the art, the tool of above-mentioned term in the present invention can be understood with concrete condition
Body meaning.The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although right with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those of skill in the art that it still can be to recorded in previous embodiment
Technical solution modify, or part of technical characteristic is replaced on an equal basis；And these are modified or replaceed, not
The essence of corresponding technical solution is set to be detached from the spirit and scope of technical solution of various embodiments of the present invention.
Claims (10)
1. a kind of centralized liquid release device, including centrifugal chip (1), the centrifugal chip (1) has rotation center
(2), which is characterized in that be provided at least one set of relieving mechanism on the centrifugal chip (1), the relieving mechanism includes liquid
Entrance (3), vesica position-limit mechanism (4), pressing mechanism (5), liquid storage vesica (6), the liquid inlet (3) and the centrifugal core
Runner connection in piece (1), the vesica position-limit mechanism (4) are fixed at the liquid inlet (3) far from the rotation
The side of the heart (2), the pressing mechanism (5) are slidably arranged in the one of the liquid inlet (3) close the rotation center (2)
Side, the liquid storage vesica (6) are arranged between the vesica position-limit mechanism (4) and the pressing mechanism (5).
2. a kind of centralized liquid release device according to claim 1, which is characterized in that the vesica position-limit mechanism
(4) puncture structure (7) towards the liquid storage vesica (6) is provided on.
3. a kind of centralized liquid release device according to claim 2, which is characterized in that the puncture structure (7) is leaned on
The bottom of the nearly liquid storage vesica (6).
4. a kind of centralized liquid release device according to claim 1, which is characterized in that the centrifugal chip (1)
On be provided with fixing groove (8), vesica position-limit mechanism (4) lower end is removably disposed on fixing groove (8).
5. a kind of centralized liquid release device according to claim 4, which is characterized in that the vesica position-limit mechanism
(4) lower end covers a part of the liquid inlet (3).
6. a kind of centralized liquid release device according to claim 1, which is characterized in that the vesica position-limit mechanism
(4) it is provided with U-shaped opening (9) towards the side of the liquid storage vesica (6), the liquid storage vesica (6) and/or the pressing mechanism
(5) shape is matched with the U-shaped opening (9).
7. a kind of centralized liquid release device according to claim 6, which is characterized in that the vesica position-limit mechanism
(4) upper end is provided with limit plate (10).
8. a kind of centralized liquid release device according to claim 1, which is characterized in that the vesica position-limit mechanism
(4) inner wall towards the side of the liquid storage vesica (6) is provided with hydrophobic layer.
9. a kind of centralized liquid release device according to claim 1, which is characterized in that the vesica position-limit mechanism
(4) inner wall towards the side of the liquid storage vesica (6) is incline structure, and the upper end of the incline structure is than lower end closer to institute
State rotation center (2).
10. a kind of centralized liquid release device according to claim 1, which is characterized in that the vesica position-limit mechanism
(4) and/or on the pressing mechanism (5) it is provided with magnetic mechanism.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111203291A (en) * | 2020-04-18 | 2020-05-29 | 博奥生物集团有限公司 | Liquid storage controlled release device and biological detection chip |
| CN111607484A (en) * | 2020-05-22 | 2020-09-01 | 东莞市东阳光诊断产品有限公司 | Nucleic acid amplification device and method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103357521A (en) * | 2012-04-04 | 2013-10-23 | 罗伯特·博世有限公司 | Revolver component for a reagent container |
| CN104399540A (en) * | 2014-12-10 | 2015-03-11 | 天津微纳芯科技有限公司 | Centrifugal effect-based micro-fluidic chip liquid reagent releasing method |
| CN105667852A (en) * | 2014-11-20 | 2016-06-15 | 绍兴普施康生物科技有限公司 | Reagent pre-packing device |
| US20180056295A1 (en) * | 2016-08-30 | 2018-03-01 | Capitalbio Ehealth Science & Technology (Beijing) Co., Ltd. | Liquid storage and release assembly and liquid storage and release chip |
| CN108489904A (en) * | 2018-04-28 | 2018-09-04 | 东莞德益生物医疗科技有限公司 | A kind of reaction vessel for detection |
| CN109847820A (en) * | 2019-04-18 | 2019-06-07 | 天津诺迈科技有限公司 | The pre-packaged device of micro-fluidic chip and application method |
| CN210357213U (en) * | 2019-06-10 | 2020-04-21 | 东莞东阳光医疗智能器件研发有限公司 | Centrifugal liquid releasing device |
-
2019
- 2019-06-10 CN CN201910496794.8A patent/CN110302850A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103357521A (en) * | 2012-04-04 | 2013-10-23 | 罗伯特·博世有限公司 | Revolver component for a reagent container |
| CN105667852A (en) * | 2014-11-20 | 2016-06-15 | 绍兴普施康生物科技有限公司 | Reagent pre-packing device |
| CN104399540A (en) * | 2014-12-10 | 2015-03-11 | 天津微纳芯科技有限公司 | Centrifugal effect-based micro-fluidic chip liquid reagent releasing method |
| US20180056295A1 (en) * | 2016-08-30 | 2018-03-01 | Capitalbio Ehealth Science & Technology (Beijing) Co., Ltd. | Liquid storage and release assembly and liquid storage and release chip |
| CN108489904A (en) * | 2018-04-28 | 2018-09-04 | 东莞德益生物医疗科技有限公司 | A kind of reaction vessel for detection |
| CN109847820A (en) * | 2019-04-18 | 2019-06-07 | 天津诺迈科技有限公司 | The pre-packaged device of micro-fluidic chip and application method |
| CN210357213U (en) * | 2019-06-10 | 2020-04-21 | 东莞东阳光医疗智能器件研发有限公司 | Centrifugal liquid releasing device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111203291A (en) * | 2020-04-18 | 2020-05-29 | 博奥生物集团有限公司 | Liquid storage controlled release device and biological detection chip |
| US11577240B2 (en) | 2020-04-18 | 2023-02-14 | Capitalbio Corporation | Liquid storage and controlled-release device and biological detection chip |
| CN111607484A (en) * | 2020-05-22 | 2020-09-01 | 东莞市东阳光诊断产品有限公司 | Nucleic acid amplification device and method |
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