CN110261592A - A kind of analyte qualitativing quantitative measuring method - Google Patents
A kind of analyte qualitativing quantitative measuring method Download PDFInfo
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- CN110261592A CN110261592A CN201910606520.XA CN201910606520A CN110261592A CN 110261592 A CN110261592 A CN 110261592A CN 201910606520 A CN201910606520 A CN 201910606520A CN 110261592 A CN110261592 A CN 110261592A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/0092—Scheduling
Abstract
The present invention provides a kind of analyte qualitativing quantitative measuring methods, this method includes automatic sample, incubation, transhipment, cleaning and detecting step, it is incubated for and cleaning carries out on raising module and cleaning module respectively, one in the differential responses stage group solid phase unit starts simultaneously on raising module and terminates to be incubated for and start simultaneously on cleaning module and terminate cleaning；It respectively walks to react the present invention is based on analyte and is both needed to be incubated for and be cleaned, the synchronous incubation of one group of solid phase unit in each step stage of reaction and synchronous cleaning are realized by transporting automatically, on the one hand single sample sequence detection is realized, each sample process is consistent, and testing result is more acurrate；On the other hand, the solid phase unit that each group is in different phase can be realized while be incubated for and clean simultaneously, and detection efficiency is substantially increased.
Description
Technical field
The present invention relates to immunoassay fields, in particular to a kind of analyte qualitativing quantitative measuring method.
Background technique
The phenomenon that specificity ligand specificity spontaneous combination, the immunoassay and nucleic acid reacted such as antigen and antibody specific
The foranalysis of nucleic acids method of base pair complementarity is widely used in multiple fields.In all analysis methods, immunoassay
Type it is the most numerous and the most jumbled.But substantially can there are four dimensions to classify, i.e. single or a variety of, out-phase or homogeneous, while handling or non-
It handles, separate and does not separate simultaneously.
Divided by the first dimension, microarray (solid phase biological chip) based on plane or film and based on flow cytometry with
Coding microball is that the liquid-phase chip of carrier can detect a variety of analytes simultaneously, and other methods are typically only capable to reach and detect a kind of quilt
Analyte.
It is divided according to the second dimension, ELISA, immunochromatography, microarray (solid phase biological chip) generation based on plane or film
The essential characteristic of table out-phase phase immunoassay.In above-mentioned technical foundation, other technologies are also derived, as microarray solid phase is exempted from
Epidemic disease analytic approach, board-like chemoluminescence method, time resolution method, fluorescence immune chromatography etc..And immunosedimentation, immunoturbidimetry, light activation
Learn shine, the chemiluminescence based on magnetic microsphere, the electrochemical luminescence based on magnetic microsphere, based on flow cytometry with coding microball
The essential characteristic being homogeneously immunoreacted is represented for the liquid-phase chip of carrier.
It is divided by third dimension, the reaction and separation of the antigen-antibody of multistep is generally included due to being immunoreacted, is adopted substantially
Meets the needs of real with two kinds of strategies.Processing is i.e. in some step of synchronization batch processing simultaneously, such as ELISA and therewith
Similar board-like chemiluminescence.Processing is advantageous in that flux is big simultaneously, and reagent cost is low.It is particularly suitable for extensive detection to appoint
Business.
The division of fourth dimension degree is pressed, sending out by the liquid-phase chip and light activation of carrier of coding microball based on flow cytometry
Light can theoretically realize that the analyte in non-separation measures.Other methods are required to separating step.
It is how accurate, efficiently, flexibly, low cost, and sustainedly and stably analyzed as a result, being the mesh that the present invention pursues
Mark.It, can there are several types of technologies close to this target in technology as above:
ELISA or board-like chemiluminescence.Design of this kind of technology based on microwell plate, relevant to a kind of analyte anti-
Former or antibody is fixed in micropore, is used together with the antigen or antibody of liquid analyzed to analyze one of liquid sample
Object.In use, user is using micropore grillage as supporting body, micropore or it is applied in combination with whole plate or with single, by incubation (antigen
Antibody response), (washing) multiple steps are separated, the testing result of the same analyte of multiple samples is finally obtained.Wherein
The separation equipment used, using vacuum pump inhale abandon hole in waste liquid, using peristaltic pump or starting pump and multichannel to micropore into
Row cleaning.General spray head and suction nozzle are multi-passage design, and 96 channel of highest is minimum also to have 8 channels.This kind of method measures sample
When, it usually also to arrange additional micropore while measure calibration object.For qualitative product, it usually needs while measuring sample
Negative control, positive control and cutoff control are measured, the qualitative results compared with control come judgement sample are passed through；For fixed
Measure product, it usually needs while measure sample measurement series of calibration product, as concentration from 0 to some numerical value analyte
Standard substance, by calculating the dense of analyte in sample with calibration object concentration and the calibration graph of calibration object signal drafting
Degree.
Relative to technology as above, microarray enzyme-linked immunization comes out, and such as quansysbio, Hua Da Ji Biai, engraves source number
The microarray enzyme-linked immunization of Kang Deng company.Using high-precision specking machine, such as the piezoelectric type specking equipment of Bio Dot company,
By it is a variety of respectively at the different relevant antigen of analyte or antibody specking to microwell plate bottom, bottom can be polystyrene
It is material or cellulose nitrate membrane material.By completing reactive moieties with the consistent mode of ELISA, finally it is different from ELISA
The method for measuring optical density, the light signal strength using the point of the micropore bottom different location of CCD record time exposure are used to
Obtain qualitative or quantitative result.
However there is no the test problems for solving single sample for this mode.This mode is depended on when measuring sample one
Surely a set of calibration object is measured, simultaneously for calculating the concentration when analyte in time sample.
Chinese patent (Suzhou three biology) solves the problems, such as above, uses plug-in type hard glass for carrier, passes through shifting
The mode of dynamic hard chip, completes detection.But this chip is there is problems, including based on glass hard chip
The corresponding flushing of design plus drying mode, structure is complicated, is unfavorable for continual cleaning.
Separation is the essential step of two methods as above, and the implementation strategy of the process is that multichannel as described above is washed
It washs.In actual application, due to the complicated component of sample, the blocking for inhaling abandoning channel is frequently problem.To understand
Certainly this problem has been developed based on the board-washing technology of centrifugal principle, such as Chinese patent CN202683525, Blue at present
The Bluewahser of Cat Bio company；And inverted microwell plate is sprayed using injector head.The method characteristic that they use is
" handling simultaneously " that is, or as unit of whole plate carries out washing or being washed as unit of whole.This mode of washing is deposited
In following problem: 1., structure is complicated, and consumes energy；2. cannot accomplish the quick wash processing of single sample；3. fluid path and sample
It directly contacts, needs to be regularly maintained, avoid cross contamination.
There is no the really lifes to being carried out in actual application for the above-mentioned all methods for having " while handling " feature
Object reaction carries out " handling simultaneously ".Because analyzed sample is still singly added, if sample size is few, sample is added
Time required for this can be ignored, but if the quantity of sample increases, first sample is arrived if the amount of sample is 100
The time interval of the last one sample will be more than 10 minutes.This mode is particularly with those test sides based on competition principle
Method.The meaning of this " handling simultaneously " is only left to reduce the amount of labour of user, for accurately measure it is harmful and without benefit, with
The increasing of sample size, the deviation of measurement result will increase.
Therefore for the accuracy of detection, ideal state is each of the result measurement from sample-adding to the end
The condition of step, which is able to maintain consistent or difference with the switch instant between step, to be ignored.However the cleaning of multistep separation and
Tens of hundreds of sample measures will increase the time of detection, severely impact the real acceptance of this perfect condition.Accelerate reaction
Speed and increase cleaning element can effectively improve real acceptance.
Current efficiently solves this problem by the homogeneous immune response of carrier of magnetic particle.Due to homogeneous reaction speed
Degree is fast, the immune response of single step can be shortened to 10 minutes, adds increased cleaning element, may be implemented more quickly
Obtain result.Such as the method disclosed in patent CN201310300162.2.First testing result can be obtained at 18 minutes, later
Every one testing result of acquisition in 20 seconds.However this method can only once obtain a kind of result.A variety of knots cannot once be obtained
Fruit.
Often a variety of results are more conducive to the judgement of disease.The measurement that several tumor markers are such as used in combination can be more
Accurate judgement or the early stage state for identifying certain tumours earlier, in another example the inspection of a variety of hypotypes of HPV, can prevent from omitting palace
The detection etc. of the pathogen of neck cancer.
Seem satisfactorily solve primary multiple analytes while survey using the liquid-phase chip technology of flow cytometry
Fixed purpose.But micro-sphere structure of this technology dependent on two kinds of fluorescent materials as label.There are also fluorescences on the surface of microballoon
Matter, nature and artificial synthesized fluorescent material itself all have hydrophobic property at present, these substances will be certainly in aqueous solution
Hair ground is assembled, and the aggregation of this hydrophobic effect has build-up effect, therefore prolonged saves or there are the inductions of environment
(such as evaporation on reagent liquid surface) all will lead to different degrees of aggregation, thus this kind of technology needs often in practical applications
Correction and calibration.
In addition the surface hydrophobicity characteristic of this bulb is unfavorable for for the sample measures high containing lipid concentration, in clinical application by
To limitation.Since all reagents are all at liquid condition, so that the freedom degree of reagent increases, the risk of variation increased, is simultaneously
Detect efficiently and with sensitivity single microballoon, it is necessary to which high speed passes through in detector in a manner of the single microballoon by hundreds of microballoons
Capillary be easy to happen plugging phenomenon so that the physics frequency of usage of capillary is excessively high.This method is increased to transport for a long time
Failure occurrence risk after row.
From the point of view of information flow, then a kind of liquid-phase chip first production of microballoon mixes, with sample into
It after row reaction, recycles external force that different arrangement of microspheres are in line, is closely divided one by one using laser excitation and microscope
Analysis.It is this from orderly, to unordered then extremely complex to orderly analytic process itself, do not conform to be suitable for it is a kind of on a large scale and
Stable analysis method.
And substep carry out solid phase chip the step of it is clear, by stablize quickly separation so that between step not mutually
Interference, it will avoid above-mentioned other methods in the various problems of practical application.The present invention is intended to provide a kind of qualitative, quantitative measurement
Method uninterruptedly can sequentially measure sample, and measurement can obtain multiple inspection results every time.
Summary of the invention
To solve the above problems, the present invention provides a kind of analyte qualitativing quantitative measuring method, the method includes certainly
Dynamic sample-adding is incubated for, cleans and detects, and the incubation and cleaning carry out on raising module and cleaning module respectively, in different
One group of solid phase unit of the stage of reaction started simultaneously on raising module and terminate be incubated for and started simultaneously on cleaning module and
Terminate cleaning.
In some preferred modes, raising module includes that at least one set of aligned solid phase unit accommodates position, often
It includes point arranging the new sample-adding position at both ends and to test sample position that group solid phase unit, which accommodates position, one group of solid single in each step stage of reaction
Member is placed in the corresponding position that accommodates and carries out incubation reaction.The solid phase unit of each stage of reaction is placed in corresponding solid phase unit to hold
Receive in position, separating and distinguishing convenient for the solid phase unit in differential responses stage, be conducive to solid phase unit multistep reaction gradually into
Row.
In some preferred modes, every group of solid phase unit of raising module accommodates position and contains N1 solid phase unit receiving position,
Wherein N1=3n or 2n, n new sample-adding positions and n are a to test sample position point column both ends.For three-step approach or two-step method, settable difference
The solid phase unit of quantity accommodates position, and incubation reaction step number is corresponding with bit quantity is accommodated.
In some preferred modes, n=1 or 2 or 3.Influence in view of loading time to Accurate Determining is loaded every time
Solid phase element number should not be excessive, newly-increased 1 or 2 or 3 solid phase units are guaranteeing that testing result is accurately basic every time
On, further increase the efficiency of detection.
In some preferred modes, after the completion of one group of solid phase unit cleaning, former raising module waits for the solid phase on test sample position
Unit is detected, remaining solid phase unit is transported to raising module and on raising module from being newly loaded position to test sample position direction
Displacement, the new solid phase unit after sample-adding is filled into stylish sample-adding position, is incubated for again.Raising module waits for the solid phase on test sample position
Unit is detected after the completion of being incubated for cleaning, remaining solid phase unit is due to completing primary incubation, cleaning process, under need to entering
The incubation of one step, cleaning process, therefore when transporting back on raising module, corresponding receiving position changes, namely original newly adds
What the solid phase unit on sample position moved into is the solid phase unit receiving position after new sample-adding position, and the new position that is loaded is for accommodating new solid phase
Unit, and the solid phase unit for carrying out final step incubation is moved into in test sample position.The solid phase in differential responses stage can be achieved in this way
Unit is incubated for simultaneously, cleaning operation, and single solid phase unit is sequentially detected.
In some preferred modes, after upper one group of solid phase unit is completed to clean and starts again at incubation, next group of solid phase
Unit cleaned, incubation step again, until last group, and circuit sequentially.Raising module solid phase unit accommodates hyte number
N2=T/t, wherein T is that raising module solid phase unit receiving position is added to addition raising module solid phase unit appearance again in solid phase unit
Receive bit interval duration, t is that one group of solid phase unit moves into raising module solid phase unit and accommodates position to next group of solid phase unit immigration and incubates
It educates module solid phase unit and accommodates bit interval duration.Since incubation reaction duration compares solid phase unit in raising module and cleaning module
Between transhipment and the cleaning duration in cleaning module to grow, by be arranged T/t group solid phase unit, may make one group of solid single
Member is completed after cleaning and being transported to raising module again, and next group of solid phase unit completes incubation reaction, can carry out cleaning operation, directly
To last group, and circuit sequentially.Due between group and group N-free diet method be connected or the linking of very short time, save entire detection
Process time greatly improves detection efficiency.
In some preferred modes, raising module includes that long slab shape incubates disk, and each group solid phase unit accommodates position along length
Direction is arranged in parallel thereon.Long slab shape incubation dish structure is simple, is successively incubated for and is cleaned convenient for each group solid phase unit.
In some preferred modes, raising module includes that disc incubates disk, and each group solid phase unit accommodates position circumferentially
Direction arranges thereon, and the line at midpoint and the center of circle that every group of solid phase unit accommodates position line accommodates position with every group of solid phase unit and connects
Vertical or every group of solid phase unit of line accommodates the extended line of position line by the center of circle.
In some preferred modes, disc incubates pan bottom and is provided with rotating device, incubates disk and is additionally provided with lid, covers
After there is body the opening for exposing one group of solid phase unit, one group of solid phase unit completion cleaning to start again at incubation, rotating device band
Dynamic temperature educates disk rotation, and lid is motionless, and lid opening exposes next group of solid phase unit, and is cleaned, incubation step again.It adopts
Disk is incubated with disc and rotating device is set, and can be reduced the cavity space incubated between disk and lid, is conducive to provide stable
Incubation reaction environment.
It is described to be transported through transhipment mould automatically the method also includes transporting step automatically in some preferred modes
Block is completed, and the transhipment module includes new sample-adding transhipment module, cleaning transhipment module, transports to test sample module, the first handgrip, the
Two handgrips, third handgrip, the new sample-adding transhipment module includes that N3 solid phase unit is newly loaded position, wherein N3=n；It is described to be measured
It includes N4 solid phase unit sample position to be measured that sample, which transports module, wherein N4=n.Transhipment module is deposited for solid phase unit in solid phase unit
Storage module, raising module, cleaning module and the transhipment for measuring intermodule in new sample-adding transhipment module, clean transhipment module, to be measured
Sample transport module, the first handgrip, the second handgrip, third handgrip collective effect under, realize solid phase unit in the automatic of intermodule
Transhipment improves detection efficiency.
In some preferred modes, the cleaning transhipment module includes that one group of N5 aligned solid phase unit holds
Receive position, wherein N5=N1, namely cleaning transhipment module solid phase unit accommodates the one group of solid phase unit in position and raising module and accommodates digit
Measure identical, it includes the n solid phase unit sample position to be measured positioned at one end that solid phase unit, which accommodates position,.
New sample-adding transhipment module and cleaning transhipment module are located at same straight line, along straight reciprocating motion, new sample-adding transhipment mould
When block moves to position I, the first handgrip, which moves along a straight line, is transferred to new sample-adding turn from solid phase unit memory module for solid phase unit
Fortune module is newly loaded on position；When moving to position II, it is loaded；When moving to position III, new sample-adding transhipment module is newly loaded
Position is newly loaded bit parallel with raising module and is aligned.
Cleaning transhipment module is along straight reciprocating motion, when block motion to first position is transported in cleaning, cleaning transhipment module
Wait for that test sample bit parallel is aligned with raising module to test sample position, the second handgrip moves along a straight line one group of solid phase unit from raising module
It is transferred in cleaning transhipment module；When cleaning transhipment module solid phase unit is completed to add washing lotion and move to the second position after cleaning,
Cleaning transhipment module waits for that test sample position waits for that test sample bit parallel is aligned with to test sample transhipment module, and third handgrip, which moves along a straight line, to be cleaned
Transhipment module waits for that the solid phase unit on test sample position is transferred to and waits on test sample position to test sample transhipment module, to test sample transhipment module transhipment
Solid phase unit to be measured to measurement module detects；When cleaning transhipment block motion to the third place, cleaning transports module close to position
New sample-adding in position III transports module, and the second handgrip moves along a straight line will be in new sample-adding transhipment module and cleaning transhipment module
Solid phase unit is transferred on raising module.
By being newly loaded transhipment module, cleaning transhipment module, to the cooperation and first of test sample transhipment block motion and position
The transferance of handgrip, the second handgrip, third handgrip realizes solid phase unit in raising module by being newly loaded position to test sample position
Displacement, realize the single full-automatic tested in sequence of solid phase unit, improve detection efficiency on the basis of improving detection accuracy.
In some preferred modes, the cleaning transhipment module includes that one group of N5 aligned solid phase unit holds
Receive position, wherein N5=N1+n, it includes that the n solid phase unit positioned at both ends is newly loaded position and n solid single that solid phase unit, which accommodates position,
Member is to test sample.
New sample-adding transhipment module is along straight reciprocating motion, and when moving to position I, the first handgrip moves along a straight line, by solid phase
Unit is transferred to new sample-adding transhipment module from solid phase unit memory module and is newly loaded on position；When moving to position II, it is loaded；
When moving to position III, new sample-adding transhipment module, which is newly loaded position, with cleaning transports module and is newly loaded bit parallel and be aligned.
The cleaning transhipment module is along straight reciprocating motion, when block motion to first position is transported in cleaning, cleaning transhipment
Module waits for that test sample position waits for that test sample bit parallel is aligned with raising module, and the second handgrip moves along a straight line one group of solid phase unit from incubation
Module is transferred in cleaning transhipment module；Cleaning transhipment module solid phase unit is completed to move to the second position after adding washing lotion and cleaning
When, cleaning transhipment module wait for test sample position with to test sample transport module wait for that test sample bit parallel is aligned, third handgrip move along a straight line by
Cleaning transhipment module waits for that the solid phase unit on test sample position is transferred to and waits on test sample position to test sample transhipment module, transports module to test sample
Solid phase unit to be measured is transported to measurement module detection；When cleaning transhipment block motion to the third place, cleaning transhipment module is new
Sample-adding position is newly loaded bit parallel with the new sample-adding transhipment module for being located at position III and is aligned, and third handgrip moves along a straight line new sample-adding
The solid phase unit that transhipment module is newly loaded on position is transferred to cleaning transhipment module and is newly loaded on position；Cleaning transhipment block motion is to the
When four positions, cleaning transhipment module, which is newly loaded position and is newly loaded bit parallel with raising module, to be aligned, the second handgrip move along a straight line by
Solid phase unit in cleaning transhipment module, which is transferred on raising module, to be incubated for.
By being newly loaded transhipment module, cleaning transhipment module, to the cooperation and first of test sample transhipment block motion and position
The transferance of handgrip, the second handgrip, third handgrip realizes solid phase unit in raising module by being newly loaded position to test sample position
Displacement, realize the single full-automatic tested in sequence of solid phase unit, improve detection efficiency on the basis of improving detection accuracy.
In some preferred modes, the sample-adding passes through sample charger automatic sample.
In some preferred modes, cleaning module includes that cleaning transhipment module disposes position, and cleaning transhipment module transhipment is solid
Phase element moves to the placement position and carries out placement and cleaning operation.It is carried out in cleaning transhipment module first motion to cleaning module
Placement and cleaning operation facilitate the solid phase unit of each stage of reaction directly and are cleaned simultaneously, easy to operate simple.
In some preferred modes, the multiple analytes of setting detect ligand in the solid phase unit, can detect simultaneously
A variety of analytes.
The invention has the advantages that:
1. analyte qualitativing quantitative measuring method of the present invention can realize analyzed sample from be loaded onto detection overall process from
Dynamicization, it is simple to operate, it is high-efficient.
2. the present invention carries out analyte measurement using solid phase unit, solid phase unit can used aloned, and can detect simultaneously
A variety of analytes compare porous plate or microwell plate etc., and operation is easier, are conducive to the consistency for keeping treatment conditions.
3. using analyte qualitativing quantitative measuring method of the present invention, it can be achieved that single sample sequence detection, overcomes a large amount of
Sample process condition caused by sample is handled simultaneously is inconsistent, the not accurate enough technical problem of measurement result, improves sample survey
Determine accuracy.
4. analyte qualitativing quantitative measuring method of the present invention be based on multistep reaction in respectively step be both needed to carry out incubation reaction and
Cleaning operation, realize differential responses stage solid phase unit and meanwhile be incubated for and while clean, detection efficiency greatly promotes.
5. analyte qualitativing quantitative measuring method of the present invention can realize that Non-intermittent is incubated between each group solid phase unit
And cleaning operation, flow time is reduced, detection efficiency is improved.
Detailed description of the invention
Fig. 1 is measurement system top view；`
Fig. 2 is measurement system perspective view；
Fig. 3 is long slab shape raising module perspective view；
Fig. 4 .1- Fig. 4 .3 is respectively one embodiment front view of disc raising module, bottom view and perspective view；
Fig. 5 .1- Fig. 5 .2 is respectively another embodiment bottom view of disc raising module and perspective view；
Fig. 6 is measurement system simplification top view；
Fig. 7 is that an embodiment cleans top view when shift module is located at first position；
Fig. 8 is that an embodiment cleans top view when shift module is located at the second position；
Fig. 9 is that an embodiment cleans top view when shift module is located at the third place；
Figure 10 is that an embodiment cleans top view when shift module is located at four positions；
Figure 11 is that measurement system simplifies perspective view；
Figure 12 is that another embodiment cleans top view when shift module is located at first position；
Figure 13 is that another embodiment cleans top view when shift module is located at the second position；
Figure 14 is that another embodiment cleans top view when shift module is located at the third place.
Specific embodiment
For ease of understanding, portion of techniques term of the present invention is described further first:
Analyte: can with the substance in conjunction with specificity ligand specificity, including but not limited to all kinds of antigens, antibody,
Polypeptide, albumen, nucleic acid etc..
Solid phase unit: solid phase unit is that one kind can carry the container of liquid, is fixed on inner surface and analyte
The ligand dot matrix of (sample) specific binding reaction.
Three-step approach, two-step method:
Enzyme mark+substrate solution is one of the measuring method enumerated in above table, it is not limited to this.
It is understandable to enable objects, features and advantages of the present invention to become apparent, with reference to the accompanying drawing to tool of the invention
Body embodiment is described in detail.
A kind of analyte qualitativing quantitative measuring method, including automatic sample, incubation, transhipment, cleaning and detection.
Automatic sample: solid phase unit 110 is transferred to new sample-adding transhipment from solid phase unit memory module 120 by the first handgrip
On the new sample-adding position 1811 of module 181, new sample-adding transhipment module 181 moves to load position, 191 (such as Figure 11 of sample charger
It is shown) sample that stores in pipette samples memory module 130, new sample-adding transhipment module 181 is then injected into newly on sample-adding position 1811
Solid phase unit 110 in.
It is incubated for: it is incubated on raising module 150 and carries out, raising module 150 includes incubating disk 151, incubates and is arranged on disk 151
Solid phase unit accommodates position.It is accommodated in position when solid phase unit to be inserted into solid phase unit, the fluid temperature inside solid phase unit will
Temperature in position is accommodated by solid phase unit to adjust, and is treated as being incubated for through constant temperature after a period of time.By being incubated for, in solid phase unit
Ligand specificity reaction will reach balance.So-called balance, exactly the reaction between assigning body will not be with the continuation of incubation time
Extend and the variation in apparent amount occurs.
Usually within the scope of certain temperature, the reaction presentation reaction temperature that ligand specificity combines is higher, reaches balance
Shorter feature of required time, as the relationship in chemical reaction between reaction speed and temperature.Using this
Principle, temperature can be turned up in the hole of raising module, to increase reaction speed, shorten the effect in reaction time.
In some embodiments, raising module 150 includes that long slab shape incubates disk 151, and it includes N2 on disk 151 that long slab shape, which incubates,
Group is arranged in parallel in the solid phase unit on its length direction and accommodates position 1510, N2=T/t, and wherein T is that incubation is added in solid phase unit
Module solid phase unit accommodates position and accommodates bit interval duration to raising module solid phase unit is added again, and t is one group on raising module
Solid phase unit moves into solid phase unit and accommodates position to next group of solid phase unit immigration solid phase unit receiving bit interval duration.Every group of solid phase
Unit accommodates position and accommodates position containing N1 aligned solid phase units, wherein N1=2n or 3n, and n preferably 1 or 2 or 3, n
New sample-adding position and n are a to test sample position point column both ends.With T=10min, it is solid to move into raising module for one group of solid phase unit on raising module
Phase element accommodates position to next group of solid phase unit and moves into the receiving of raising module solid phase unit bit interval duration t=0.5min, N1=
For 3n, n=1, as shown in Figure 1-3, long slab shape, which incubates disk 151, accommodates position 1510 containing N2=20 group solid phase unit, every group solid
Phase element accommodates position and accommodates position containing 3 aligned solid phase units, and every group of solid phase unit accommodates on the left of position as new sample-adding
Position 1511, for accommodating the solid phase unit in first step reaction in three-step approach, centre accommodates position for accommodating in three-step approach
In the solid phase unit of second step reaction, right side is to test sample position 1512, for accommodating in three-step approach in final step reaction
Solid phase unit.Therefore it is newly to be loaded position 1511 that long slab shape, which incubates 20 solid phase units of disk first row and accommodates position, and third column 20
It is to test sample position that solid phase unit, which accommodates position,.
Solid phase unit after adding sample is added on the incubation disk 151 of raising module 150 with the time interval of 0.5min, the
1 solid phase unit, which is placed in, to be incubated on disk first row the 1st new sample-adding position, and the 2nd solid phase unit, which is placed in, incubates disk first row
On 2nd new sample-adding position, incubated on disk first row the 20th new sample-adding position until the 20th solid phase unit is placed in.When the 20th
After solid phase unit is placed, the 1st solid phase unit just completes first time incubation reaction, and it is clear that the 1st solid phase unit is moved out of progress
Second step reaction reagent is washed and added, the 1st solid phase unit of raising module secondary series is then moved into and accommodates on position, at the same time the 21st
A solid phase unit is placed on first row the 1st new sample-adding position, this process used time 0.5min, the 2nd solid phase unit is complete at this time
It at incubation, carries out cleaning and adding second step reaction reagent, then moves into the 2nd solid phase unit of raising module secondary series and accommodate position
On, the 22nd solid phase unit is placed on first row the 2nd new sample-adding position at the same time, and so on, until the 40th is solid
Phase element, which is placed in, to be incubated on disk first row the 20th new sample-adding position.After the 40th solid phase unit is placed, the 1st and the 21st
Solid phase unit is just respectively completed second and first time incubation reaction, and the 1st and the 21st solid phase unit remove progress simultaneously
It cleans and adds third step and second step reaction reagent respectively, then move into raising module third respectively and arrange the 1st and secondary series the 1st
A solid phase unit accommodates on position, and the 41st solid phase unit is placed on first row the 1st new sample-adding position at the same time, this process
Used time 0.5min.And so on, it is incubated on disk first row the 20th new sample-adding position until the 60th solid phase unit is placed in.When
After 60 solid phase units are placed, the 1st, the 21st and the 41st solid phase unit is just respectively completed for the third time, second and the
Incubation reaction, the 1st, the 21st and the 41st solid phase unit removes simultaneously to be cleaned, and after the completion of cleaning, the 1st solid
Phase element is detected, and the 21st and the 41st solid phase unit are separately added into third step and second step reaction reagent, is then distinguished
It moves into raising module third to arrange on the 1st and the 1st solid phase unit receiving position of secondary series, at the same time the 61st solid phase unit quilt
It is placed on first row the 1st new sample-adding position, this process used time 0.5min, then the 2nd, the 22nd, the 42nd solid phase unit
Relevant operation is carried out, and is circuited sequentially.Single sample tested in sequence is thereby realized, while the respectively step reaction in three-step approach
One group of solid phase unit in stage is incubated for simultaneously and cleaning, substantially increases detection efficiency.
In some embodiments, with T=10min, one group of solid phase unit moves into raising module solid phase unit on raising module
It accommodates position to next group of solid phase unit and moves into the receiving of raising module solid phase unit bit interval duration t=0.5min, N1=2n, n=1
For.Long slab shape incubates disk 151 and accommodates position 1510 containing N2=20 group solid phase unit arranged in parallel, and every group of solid phase unit accommodates
Position accommodates position containing 2 aligned solid phase units, and every group of solid phase unit is accommodated on the left of position as new sample-adding position 1511, be used for
The solid phase unit in first step reaction in two-step method is accommodated, right side is to be in test sample position 1512 for accommodating in two-step method
The solid phase unit of second step reaction.Therefore it is new sample-adding position that long slab shape, which incubates 20 solid phase units of disk first row and accommodates position,
1511, it is to test sample position that 20 solid phase units of secondary series, which accommodate position,.
Solid phase unit after adding sample is added on the incubation disk 151 of raising module 150 with the time interval of 0.5min, the
1 solid phase unit, which is placed in, to be incubated on disk first row the 1st new sample-adding position, and the 2nd solid phase unit, which is placed in, incubates disk first row
On 2nd new sample-adding position, incubated on disk first row the 20th new sample-adding position until the 20th solid phase unit is placed in.When the 20th
After solid phase unit is placed, the 1st solid phase unit just completes first time incubation reaction, and it is clear that the 1st solid phase unit is moved out of progress
Second step reaction reagent is washed and added, then moves into raising module secondary series the 1st on test sample position, at the same time the 21st solid phase
Unit is placed on first row the 1st new sample-adding position, this process used time 0.5min, the 2nd solid phase unit is completed to be incubated at this time,
It carries out cleaning and adding second step reaction reagent, then moves into raising module secondary series the 2nd on test sample position, at the same time the 22nd
A solid phase unit is placed on first row the 2nd new sample-adding position, and so on, until the 40th solid phase unit is placed in incubation
On disk first row the 20th new sample-adding position.After the 40th solid phase unit is placed, the 1st and the 21st solid phase unit are just distinguished
Second and first time incubation reaction are completed, the 1st and the 21st solid phase unit remove cleaned simultaneously, after the completion of cleaning,
1st solid phase unit is detected, and second step reaction reagent is added in the 21st solid phase unit, then moves into raising module secondary series
1st to which on test sample position, the 41st solid phase unit is placed on first row the 1st new sample-adding position at the same time, this process is used
When 0.5min, then the 2nd, the 22nd progress relevant operation, and circuit sequentially.Single sample is thereby realized sequentially to examine
It surveys, while being in two-step method one group of solid phase unit for respectively walking the stage of reaction while being incubated for and cleaning, substantially increase detection effect
Rate.
In some embodiments, as shown in Fig. 4 .1- Fig. 5 .2, raising module 150 ' includes disc incubation disk 151 ', until
Few one group of solid phase unit accommodates position and arranges thereon by circumferential direction, and every group of solid phase unit receiving position is aligned, line
Midpoint and the line in the center of circle and every group of solid phase unit accommodate the extension that line vertical or every group of solid phase unit in position accommodates position line
Line is by the center of circle.Solid phase unit is similar on long slab shape incubation disk in the incubation on disc incubation disk 151 ', solid at one group
Phase element completes cleaning, and after moving into raising module solid phase unit receiving position, disc incubates the rotating device of 151 ' bottom of disk
152 ', which drive disc to incubate disk 151 ', rotates, and lid 153 ' is motionless, so that next group of solid phase unit exposes lid 153 '
154 ' place of opening, to carry out the operation of next step.
Transhipment: using automatic transhipment, it is transported through transhipment module automatically and carries out, as shown in fig. 6, the transhipment module includes
New sample-adding transhipment module 181, cleaning transhipment module 182, to test sample transhipment module 183, the first handgrip 184, the second handgrip 185,
Third handgrip 186, the new sample-adding transhipment module 181 includes that N3 solid phase unit is newly loaded position 1811, wherein N3=n；It is described
Include N4 solid phase unit sample position 1831 to be measured to test sample transhipment module 183, wherein N4=n.
In some embodiments, the cleaning transhipment module includes that one group of N5 aligned solid phase unit accommodates
Position, wherein N5=N1+n, solid phase unit receiving position include that the n solid phase unit positioned at both ends is newly loaded position and n solid phase unit
To test sample.By taking n=1 as an example, metal is incubated for 151 every groups of disk and accommodates comprising 3 solid phase units in raising module 150 as shown in Figure 6
Position, Far Left are new sample-adding position 1511, and rightmost is to test sample position 1512；Cleaning transhipment module 182 includes 4 receiving positions, most
The left side is new sample-adding position 1821, and rightmost is to test sample position 1822；New sample-adding transhipment module 181 includes 1 new sample-adding position 1811,
To test sample transhipment module 183 containing 1 to test sample position 1831.Cleaning transhipment module 182, new sample-adding transport module 181, to test sample
Module 183 is transported along straight reciprocating motion, and the first handgrip 184 has 1 solid phase unit picks position, can transport along linear reciprocation
It is dynamic.Second handgrip 185 has 3 solid phase unit picks positions, can be along incubation 151 length direction straight reciprocating motion of disk.Third is grabbed
Hand 186 has 1 solid phase unit picks position, along straight reciprocating motion.
Cleaning transhipment module includes 4 positions in moving along a straight line, when being located at first position, as shown in fig. 7, cleaning turns
Module 182 is transported to test sample position 1822 and raising module 150 to 1512 parallel alignment of test sample position, at this moment the second handgrip 185 crawl is incubated
Educate 3 in module 150 and be in the solid phase units in differential responses stage, and move along a straight line to cleaning transhipment module 182 just on
Solid phase unit on raising module 150 is aligned one by one and is transported on the cleaning transhipment receiving of module 182 position by side.Then cleaning turns
It transports module 182 and transports solid phase unit to cleaning module 160, complete filling washing lotion and cleaning, the first handgrip 184 grabs during this period
Solid phase unit 110 in solid phase storage module 120 and along linear to the new sample-adding for being located at position I transport module 181 newly plus
On sample position 1811, then new sample-adding transhipment module 181 moves to the position II of sample-adding.Cleaning transhipment module 182 is subsequently transferred to
The second position measures outside module, cleaning as shown in figure 8, transporting module 183 to test sample simultaneously and moving to out of measurement module 170
Transport module 182 to test sample position 1822 (being located at the lower section of third handgrip 186, do not show in Fig. 8) with to test sample transport module 183 to
1831 parallel alignment of test sample position, the crawl cleaning transhipment module 182 of third handgrip 186 is to the solid phase unit on test sample position 1822 and edge
Linear transports module 183 to transport solid phase unit along straight line to test sample transhipment module 183 on test sample position 1831 to test sample
It moves in measurement module 170 and is measured.When cleaning transhipment module 182 moves to the third place, as shown in figure 9, completing to add
The solid phase unit of sample is transferred to position III by new sample-adding transhipment module 181, the module 182 of cleaning transhipment at this time newly sample-adding position 1821 with
Newly (the newly sample-adding position 1821 of module 182 is transported in cleaning to sample-adding 1811 parallel alignment of position to new sample-adding transhipment module 181 positioned at position III
Newly be located at below third handgrip 186 sample-adding position 1811 with new sample-adding transhipment module 181, do not shown in Fig. 9), third handgrip 186 from
New sample-adding transhipment module 181, which is newly loaded on position 1811 to grab solid phase unit and be transferred to cleaning transhipment module 182, is newly loaded position
On 1821.Then cleaning transhipment module 182 moves to the 4th position, and as shown in Figure 10, the module 182 of cleaning transhipment at this time is newly loaded
Position 1821 is newly loaded 1511 parallel alignment of position with raising module 150, and (cleaning transhipment module 182 newly grab positioned at second by sample-adding position 1821
Below hand 185, do not shown in Figure 10), cleaning is transported the solid phase unit picks in module 182 and an a pair by the second handgrip 185
It is transferred on raising module 150 together, the solid phase unit that cleaning transhipment module 182 is newly loaded on position 1821 after transfer, which is located at, is incubated for mould
Block 150 is newly on sample-adding position 1511.Then, the second handgrip grabs next group of solid phase unit and carries out aforesaid operations, and circuits sequentially.
In some embodiments, the cleaning transhipment module 182 includes that one group of N5 aligned solid phase unit holds
Receive position, wherein N5=N1, namely cleaning transhipment 182 solid phase unit of module accommodates the 150 1 groups of solid phase units in position and raising module and holds
Bit quantity of receiving is identical, and it includes positioned at the n solid phase unit sample position 1512 to be measured of one end that cleaning transhipment module solid phase unit, which accommodates position,.
By taking n=1 as an example, as shown in figs. 12-14,150 every groups of raising module accommodate position comprising 3 solid phase units, and Far Left is new sample-adding
Position 1511, rightmost are to test sample position 1512；Cleaning transhipment module 182 includes 3 receiving positions, and rightmost is to test sample position
1822；New sample-adding transhipment module 181 includes 1 new sample-adding position 1811, includes 1 to test sample position to test sample transhipment module 183
1831.Cleaning transhipment module 182, new sample-adding transhipment module 181, to test sample transhipment module 183 along straight reciprocating motion, first
Handgrip 184 is located at right above the solid phase unit in solid phase unit memory module 120, has 1 solid phase unit picks position, can be along straight
Line moves back and forth.Second handgrip 185 is located at long slab shape in raising module 150 and incubates right above disk 151, has 3 solid phase units
Position is grabbed, it can be along incubation 151 length direction straight reciprocating motion of disk.Third handgrip 186 has 1 solid phase unit picks position, edge
Straight reciprocating motion.
Cleaning transhipment module 182 includes 3 positions in moving along a straight line, when being located at first position, as shown in figure 12, clearly
Transhipment module 182 is washed to test sample position 1822 and raising module 150 to 1512 parallel alignment of test sample position, at this moment the second handgrip 185 is grabbed
It takes 3 on raising module 150 to be in the solid phase unit in differential responses stage, and moves along a straight line and transport module 182 to cleaning
Solid phase unit is aligned one by one and is transferred in cleaning transhipment module 182 by surface, and raising module 150 is to test sample position after transfer
Solid phase unit on 1512 is located at cleaning transhipment module 182 on test sample position 1822.Then cleaning transhipment module 182 transports solid phase
Unit completes filling washing lotion and cleaning, during this period in the first handgrip 184 crawl solid phase storage module 120 to cleaning module 160
Solid phase unit 110 and along linear to be located at position I new sample-adding transport module 181 newly sample-adding position along 1811, then newly
Sample-adding transhipment module 181 moves to the position II of sample-adding, this stylish sample-adding transhipment module 181 is located at cleaning transhipment module 182
On same straight line, and newly sample-adding position 1811 is newly loaded that position 1511 is parallel to be staggered with raising module 150 to new sample-adding transhipment module 181.
Cleaning transhipment module 182 then moves to the second position (as shown in figure 13), while transporting module 183 from measurement module to test sample
It is moved in 170 outside measurement module 170, cleaning transhipment module 182 (is located at 186 lower section of third handgrip, Figure 13 to test sample position 1822
In do not show) transport module 183 to 1831 parallel alignment of test sample position with to test sample, the crawl cleaning transhipment module of third handgrip 186
182 transport module 183 on test sample position 1831 to test sample to the solid phase unit on test sample position 1822 and along linear, to be measured
Sample transhipment module 183 transport solid phase unit move along a straight line to measurement module 170 in be measured.Cleaning transhipment module 182 is transported
When moving to the third place, as shown in figure 14, the new solid phase unit for completing sample-adding is transferred to position by new sample-adding transhipment module 181
III, this stylish sample-adding transhipment module 181 and cleaning transport module 182 and are located at same straight line, and new sample-adding transhipment module 181 newly adds
Newly (newly sample-adding position 1811 is located at the to new sample-adding transhipment module 181 to sample-adding 1511 parallel alignment of position for sample position 1811 and raising module 150
Below two handgrip 185, do not shown in Figure 14), new sample-adding transhipment module 181 is newly loaded position 1811 and transports module 182 close to cleaning,
New sample-adding transhipment module 181 is newly loaded the solid phase unit picks on position 1811 and cleaning transhipment module 182 simultaneously by the second handgrip 185
Alignment is transferred on raising module 150 one by one, and newly sample-adding transhipment module 181 is newly loaded the solid phase unit position on position 1811 after transfer
In on raising module 150 newly sample-adding position 1511.Then, next group of solid phase unit progress aforesaid operations of the second handgrip crawl, and according to
Secondary circulation.
Cleaning: cleaning carries out on cleaning module 160, and cleaning module 160 includes that cleaning transhipment module disposes position.Cleaning turns
Fortune module transports one group of solid phase unit and moves to predetermined position, and washing lotion is filled into solid by washing lotion charger 193 (as shown in figure 11)
In phase element, washing lotion filling is finished, and cleaning transhipment module transhipment solid phase unit moves in cleaning module 160 on placement position, from
The heart dries washing lotion, after completing primary cleaning, repeats and carries out washing lotion filling and eccentric cleaning, wash number is no more than 3 times.
Measurement: measurement carries out in measurement module, and solid phase unit is transported in measurement module by transporting module to test sample,
Substrate solution is injected, starts the collection for carrying out solid phase unit bottom signal at the time of turntable turns at object lens mouth of taking pictures.The receipts
Collection will be completed within the time that is specific but rotating again no more than turntable.
Reagent adding: in some embodiments, as shown in figure 11, reaction reagent is filled into solid phase by reagent charger 192
In unit, for three-step approach, reagent charger includes the first reagent charger 1921 and the filling for filling second step reaction reagent
Second reagent charger 1922 of third step reaction reagent；The automatic filling of reaction reagent can be realized by reagent charger.
Although present disclosure is as above, present invention is not limited to this.Anyone skilled in the art are not departing from this
It in the spirit and scope of invention, can make various changes or modifications, therefore protection scope of the present invention should be with claim institute
Subject to the range of restriction.
Claims (21)
1. a kind of analyte qualitativing quantitative measuring method, which is characterized in that the method includes automatic sample, incubation, cleanings
And detection, the incubation and cleaning carry out on raising module and cleaning module respectively, one in the differential responses stage group is solid
Phase element starts simultaneously on raising module and terminates to be incubated for and start simultaneously on cleaning module and terminate cleaning.
2. the method as described in claim 1, which is characterized in that the raising module, which includes that at least one set is aligned, to be consolidated
Phase element accommodates position, and it includes point new sample-adding position at column both ends and to test sample position that every group of solid phase unit, which accommodates position, in the reaction of each step
One group of solid phase unit in stage is placed in the corresponding position that accommodates and carries out incubation reaction.
3. method according to claim 2, which is characterized in that every group of solid phase unit of the raising module accommodates position and contain N1
Solid phase unit accommodates position, wherein N1=2n or 3n, and n new sample-adding positions and n are a to test sample position point column both ends.
4. method as claimed in claim 3, which is characterized in that after the completion of one group of solid phase unit cleaning, former raising module is to be measured
Solid phase unit on sample position is detected, remaining solid phase unit be transported to raising module and on raising module from be newly loaded position to
It is shifted to test sample position direction, the new solid phase unit after sample-adding is filled into stylish sample-adding position is incubated for again.
5. method as claimed in claim 4, which is characterized in that complete cleaning in upper one group of solid phase unit and start again at incubation
Afterwards, next group of solid phase unit cleaned, incubation step again, until last group, and circuit sequentially.
6. method as claimed in claim 5, which is characterized in that the raising module solid phase unit accommodates hyte number N2=T/t,
Wherein T is that raising module solid phase unit receiving position is added to addition raising module solid phase unit receiving bit interval again in solid phase unit
Duration, t are that one group of solid phase unit immigration raising module solid phase unit receiving position is solid to next group of solid phase unit immigration raising module
Phase element accommodates bit interval duration.
7. method as claimed in claim 6, which is characterized in that the raising module includes that long slab shape incubates disk, each group solid phase
It is arranged in parallel along its length thereon that unit accommodates position.
8. method as claimed in claim 6, which is characterized in that the raising module includes that disc incubates disk, each group solid phase
Unit accommodates position and arranges along circumferential direction thereon.
9. method according to claim 8, which is characterized in that every group of solid phase unit accommodates the midpoint of position line and the company in the center of circle
Line accommodates vertical or every group of solid phase unit of position line with every group of solid phase unit and accommodates the extended line of position line by the center of circle.
10. method according to claim 8, which is characterized in that the incubation pan bottom is provided with rotating device, incubates disk also
Equipped with lid, lid has the opening for exposing one group of solid phase unit.
11. method as claimed in claim 10, which is characterized in that upper one group of solid phase unit completes cleaning and starts again at incubation
Afterwards, rotating device, which drives, incubates disk rotation, and lid is motionless, and lid opening exposes next group of solid phase unit, is cleaned, again
Incubation step.
12. the method as described in one of claim 1-11, which is characterized in that the method also includes transporting step automatically, institute
It states and is transported through transhipment module completion automatically, the transhipment module transports module, to test sample including cleaning transhipment module, new be loaded
Transport module, the first handgrip, the second handgrip, third handgrip；New sample-adding transhipment module includes N3 new sample-adding positions, is transported to test sample
Module includes N4 to test sample position, wherein N3=N4=n.
13. method as claimed in claim 12, which is characterized in that the cleaning transhipment module includes the one group of N5 row of being in line
The solid phase unit of column accommodates position, wherein N5=N1, and it includes the n solid phase unit sample to be measured positioned at one end that solid phase unit, which accommodates position,
Position.
14. method as claimed in claim 13, which is characterized in that new sample-adding transhipment module is located at same with cleaning transhipment module
Straight line, new sample-adding transhipment module is along straight reciprocating motion, and when new sample-adding transhipment block motion to position I, the first handgrip is by solid phase
Unit is transferred to new sample-adding transhipment module from solid phase unit memory module and is newly loaded on position；When moving to position II, it is loaded；
When moving to position III, new sample-adding transhipment module, which is newly loaded position and is newly loaded bit parallel with raising module, to be aligned.
15. method as claimed in claim 14, which is characterized in that the cleaning transhipment module is along straight reciprocating motion, cleaning
When transporting block motion to first position, cleaning transhipment module waits for that test sample position waits for that test sample bit parallel is aligned with raising module, second
One group of solid phase unit is transferred in cleaning transhipment module by handgrip from raising module；Cleaning transhipment module solid phase unit is completed plus is washed
When moving to the second position after liquid and cleaning, cleaning transhipment module waits for that test sample bit parallel pair is waited for to test sample transhipment module in test sample position
Together, third handgrip will cleaning transhipment module wait for the solid phase unit on test sample position be transferred to test sample transhipment module wait on test sample position,
Solid phase unit to be measured is transported to measurement module detection to test sample transhipment module；When cleaning transhipment block motion to the third place,
Cleaning transhipment module is immediately adjacent to the new sample-adding transhipment module of position III, and the second handgrip will newly be loaded transhipment module and cleaning transhipment
Solid phase unit in module is transferred on raising module.
16. method as claimed in claim 12, which is characterized in that the cleaning transhipment module includes the one group of N5 row of being in line
The solid phase unit of column accommodates position, wherein N5=N1+n, and it includes that the n solid phase unit positioned at both ends is newly loaded that solid phase unit, which accommodates position,
Position and n solid phase unit sample position to be measured.
17. the method described in claim 16, which is characterized in that it is characterized in that, new sample-adding transhipment module is along linear reciprocation
Movement, when moving to position I, it is new that solid phase unit is transferred to new sample-adding transhipment module from solid phase unit memory module by the first handgrip
It is loaded on position；When moving to position II, it is loaded；When moving to position III, new sample-adding transhipment module is newly loaded position and cleaning
Transhipment module is newly loaded bit parallel alignment.
18. method as claimed in claim 17, which is characterized in that the cleaning transhipment module is along straight reciprocating motion, cleaning
When transporting block motion to first position, cleaning transhipment module waits for that test sample position waits for that test sample bit parallel is aligned with raising module, second
One group of solid phase unit is transferred in cleaning transhipment module by handgrip from raising module；Cleaning transhipment module solid phase unit is completed plus is washed
When moving to the second position after liquid and cleaning, cleaning transhipment module waits for that test sample bit parallel pair is waited for to test sample transhipment module in test sample position
Together, third handgrip will cleaning transhipment module wait for the solid phase unit on test sample position be transferred to test sample transhipment module wait on test sample position,
Solid phase unit to be measured is transported to measurement module detection to test sample transhipment module；When cleaning transhipment block motion to the third place,
Cleaning transhipment module, which is newly loaded position, transports module with the new sample-adding for being located at position III and is newly loaded bit parallel and is aligned, and third handgrip will be new
The solid phase unit that sample-adding transhipment module is newly loaded on position is transferred to cleaning transhipment module and is newly loaded on position；Cleaning transhipment block motion
When to four positions, cleaning transhipment module, which is newly loaded position and is newly loaded bit parallel with raising module, to be aligned, and the second handgrip will clean turn
Solid phase unit in fortune module, which is transferred on raising module, to be incubated for.
19. the method as described in claim 1, which is characterized in that the sample-adding passes through sample charger automatic sample.
20. method as claimed in claim 12, which is characterized in that cleaning module includes that cleaning transhipment module disposes position, cleaning
Transhipment module transhipment solid phase unit moves to the placement position and carries out placement and cleaning operation.
21. the method as described in claim 1, which is characterized in that the multiple analyte detections of setting are matched in the solid phase unit
Body.
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| CN111398511A (en) * | 2020-04-03 | 2020-07-10 | 杭州布封科技有限公司 | Detection device and detection method for detecting helicobacter pylori |
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