CN110174152A - A method of using the measurement of breathing cabin and calculating milk cow gastrointestinal tract discharge of methane - Google Patents
A method of using the measurement of breathing cabin and calculating milk cow gastrointestinal tract discharge of methane Download PDFInfo
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- CN110174152A CN110174152A CN201910314381.3A CN201910314381A CN110174152A CN 110174152 A CN110174152 A CN 110174152A CN 201910314381 A CN201910314381 A CN 201910314381A CN 110174152 A CN110174152 A CN 110174152A
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- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F22/00—Methods or apparatus for measuring volume of fluids or fluent solid material, not otherwise provided for
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Abstract
The invention discloses a kind of methods for measuring using breathing cabin and calculating milk cow gastrointestinal tract discharge of methane, choose more bovine cows, and milk cow is caught up with into breathing cabin, feed TMR basal diet, feed twice in continuous 23 hours minutes；Methane determination is carried out respectively to each cow head, in continuous 23 hours minutes, every setting measuring interval time measures the methane concentration of exhaust pipe discharge, and then the 1h time sweeps the health in breathing cabin；Calculate methane production；In being measured using breathing cabin to methane production, minute is spaced within 3h, using the methane data for closing on value method supplement missing, can more accurately calculate methane production.
Description
Technical field
The present invention relates to common greenhouse gases (methane) determination techniques fields, and in particular to it is a kind of using breathing cabin measurement and
The method for calculating milk cow gastrointestinal tract discharge of methane.
Background technique
In recent years, increasingly serious with global warming situation, the discharge that people increasingly pay close attention to greenhouse gases is asked
Topic.Breathing cabin method is to measure one of the common methods of ruminant gastrointestinal tract discharge of methane.Breathing cabin system is usually by several
Cabin composition is breathed, the methane concentration in each breathing cabin is measured in turn at regular intervals by analyzer.But
When milk cow, which is placed in breathing cabin, to be measured, the methane concentration breathed in cabin is ceaselessly to change, this right in turn
The mode for breathing methane concentration measurement in cabin may be because of the maximum value or minimum value for missing some measurement results, and to calculating
Discharge of methane has an impact.
It when using breathing cabin system to Methane determination in cabin is breathed, needs to be corrected analyzer daily, and sweeps
Health in cabin is breathed, therefore, practical measurement time deficiency is for 24 hours.Currently, mainly having " averaging method " for the data of missing and " facing
Close values method " is supplemented, which kind of is more suitable for two methods, is studied at present less.
Summary of the invention
The purpose of the present invention is be that overcoming the deficiencies of the prior art and provide a kind of utilize breathes cabin measurement and calculating milk cow
The method of gastrointestinal tract discharge of methane.
Above-mentioned purpose of the invention is achieved through the following technical solutions:
A method of using the measurement of breathing cabin and calculating milk cow gastrointestinal tract discharge of methane, comprising the following steps:
Step 1: choosing more bovine cows, milk cow is caught up with into breathing cabin, every cow head is located at different breathing cabins, feeds TMR
Basal diet, the interior feeding of continuous 23 hours minutes is twice；
Step 2 carries out Methane determination to each cow head respectively, in continuous 23 hours minutes, every setting measurement
Interval time measures the methane concentration of exhaust pipe discharge, and then the 1h time sweeps the health in breathing cabin；
Step 3 calculates time tiWith time ti-1Between methane production Δ Vi；Time tiWith time ti-1Positioned at 23 hours
Minute in, i is the serial number of the data acquisition in 23 hours minutes,
△ViFor time tiWith time ti-1Between methane production (L)；Time tiWith time ti-1Positioned at measurement in 23 hours
In time, the serial number of the data acquisition in the minute that i is 23 hours；
RgFor the gas flow rate of exhaust pipe, unit L/h；
CCiFor methane concentration in exhaust pipe, unit 106L/L；
CAiIt is 10 for methane concentration unit in open-air6L/L；
VCFor breathing cabin product, unit m3；
RiFor time tiWith time ti-1Between methane generating rate, unit L/h；
The sum of data acquisition in the minute that n is 23 hours；
V23hFor the methane production in 23 hours minutes.
A method of using the measurement of breathing cabin and milk cow gastrointestinal tract discharge of methane being calculated, further comprising the steps of:
Calculate the methane production V for 24 hours of milk cow24h:
V24h=V23h+V1h
V1h=1 hour × V23h/ 23 or V1h=1 hour × Rmi
Wherein, RmiFor the R measured for the last time in 23 hours minutesi。
A method of using the measurement of breathing cabin and milk cow gastrointestinal tract discharge of methane being calculated, when the described setting measuring interval
Between be less than or equal to 3 hours.
Compared with prior art, the present invention having the following advantages that and effect:
The technology of the present invention, which specifies, utilizes suitable time interval and calculation method measurement milk cow discharge of methane.
Detailed description of the invention
Fig. 1 is the tendency chart of the average value of the methane generating rate of the milk cow of different time intervals.Wherein, (a), (b),
(c), (d), (e), (f) are respectively that time measuring interval is corresponding for 5min, 30min, 60min, 120min, 180min, 240min
Methane generating rate average value tendency chart.
Specific embodiment
For the ease of those of ordinary skill in the art understand and implement the present invention, below with reference to embodiment to the present invention make into
The detailed description of one step, it should be understood that implementation example described herein is merely to illustrate and explain the present invention, and is not used to limit
The fixed present invention.
Embodiment 1
The present embodiment uses breathing cabin, breathes and is provided with admission line and exhaust pipe on cabin, is arranged at admission line
Have ventilating fan, be provided with air exhauster at exhaust pipe, close the ventilating fan and air exhauster in breathing cabin, by analyzer into
Port is connected to exhaust pipe, and analyzer is used to measure the methane concentration of exhaust pipe discharge, is also used to measure exhaust pipe gas
Body emission flow, is also used to measure methane concentration in open-air, when measurement, opens ventilating fan and air exhauster.
A method of using the measurement of breathing cabin and calculating milk cow gastrointestinal tract discharge of methane, comprising the following steps:
Step 1 selects 10 372 ± 15 kilograms of average weight of 3~4 years old holstein cow as experimental animal, by lotus
This smooth milk cow is caught up with into breathing cabin, and every holstein cow is located at different breathing cabins, feeds TMR basal diet, daily 09:00 and
16:00 is each primary, and each feeding time is no more than 3min, 09:00 and 16:00 and is located in continuous 23 hours minutes.
Step 2 carries out Methane determination to 10 holstein cows using breathing cabin respectively, in continuous measurement in 23 hours
In, every setting measuring interval time measures the methane concentration of exhaust pipe discharge, and then the 1h time sweeps breathing cabin
Health.The measuring interval time is set as 0.5min~180min, in the present embodiment, search time measuring interval
(i.e.0.5min, 5min, 30min, 60min, 120min, 180min, 240min) is to milk cow methane generating rate and discharge amount
Influence.
Step 3 calculates time tiWith time ti-1Between methane production Δ Vi(unit L)；Time tiWith time ti-1Position
In in 23 hours minutes, the serial number of the data acquisition in the minute that i is 23 hours,
△ViFor time tiWith time ti-1Between methane production (L)；Time tiWith time ti-1Positioned at measurement in 23 hours
In time, the serial number of the data acquisition in the minute that i is 23 hours；
RgFor the gas flow rate (L/h) of exhaust pipe；
CCiFor methane concentration (10 in exhaust pipe6L/L)；
CAiFor methane concentration (10 in open-air6L/L)；
VCFor breathing cabin product (m3)；
RiFor time tiWith time ti-1Between methane generating rate (L/h)；
The sum of data acquisition in the minute that n is 23 hours；
V23hFor the methane production in 23 hours minutes.
Methane generating rate tendency chart is formed, the abscissa of methane generating rate tendency chart is the time, and ordinate is methane
Generating rate.
Table 1 sets influence of the measuring interval time to milk cow methane production
In table 1, subscript a, b, c, d, e, f difference mark, the identical upper target data difference of file is unobvious, target in difference
Data difference is obvious (P < 0.05).
Methane production is the average value of the methane production in 23 hours minutes of 10 holstein cows.
Standard error refers to the standard error between the data of file.
Time interval influences: influence of the minute interval to methane production and generating rate.
Milk cow influences: influence of the milk cow individual to methane production and generating rate.
Test result: the methane generating rate in breathing cabin is not stop to change (Fig. 1) within 23h.Methane generates speed
The maximum value of the rate mainly 1-2h after feeding, then gradually decreases.In the case where milk cow feeds twice daily, can scheme
Two apparent peak values are found out in 1.
When setting measuring interval selection of time difference, i.e., when measurement frequency reduces, minute interval increases, and methane is raw
Significant change has occurred at rate trend figure.For example, when the setting measuring interval time increases to 4h by 0.5min, by initially bright
Two aobvious peak values, are reduced to 1.In addition, increasing the setting measuring interval time, the maximum of methane generating rate is significantly reduced
Value, increases methane generating rate minimum value.
Although setting the increase of measuring interval time, the maximum value and minimum value of methane generating rate changed dramatically,
When setting the measuring interval time increases to 180min by 0.5min, methane production does not have significant changes.When with 0.5min measurement
Between be spaced and compare, when minute interval is 4h, methane production is dramatically increased.
The result shows that: in being measured to methane production using breathing cabin, the measuring interval time is set within 3h, it can
More accurately calculate methane production.
Embodiment 2
The present embodiment uses breathing cabin, breathes and is provided with admission line and exhaust pipe on cabin, is arranged at admission line
Have ventilating fan, be provided with air exhauster at exhaust pipe, close the ventilating fan and air exhauster in breathing cabin, by analyzer into
Port is connected to exhaust pipe, and analyzer is used to measure the methane concentration of exhaust pipe discharge, is also used to measure exhaust pipe gas
Body emission flow, is also used to measure methane concentration in open-air, when measurement, opens ventilating fan and air exhauster.
A method of using the measurement of breathing cabin and calculating milk cow gastrointestinal tract discharge of methane, comprising the following steps:
Step 1 selects 10 372 ± 15 kilograms of average weight of 3~4 years old holstein cow as experimental animal, by lotus
This smooth milk cow is caught up with into breathing cabin, and every holstein cow is located at different breathing cabins, feeds TMR basal diet, daily 09:00 and
16:00 is each primary, and each feeding time is no more than 3min.
Step 2 carries out Methane determination to 10 holstein cows using breathing cabin respectively, in continuous measurement in 23 hours
In, every setting measuring interval time measures the methane concentration of exhaust pipe discharge, and then the 1h time sweeps breathing cabin
Health.The measuring interval time is set as 0.5min~180min, in the present embodiment, the setting measuring interval time is respectively
0.5min、5min、30min、60min、120min、180min、240min。
Step 3 calculates time tiWith time ti-1Between methane production Δ Vi(unit L)；Time tiWith time ti-1Position
In in 23 hours minutes, the serial number of the data acquisition in the minute that i is 23 hours,
△ViFor time tiWith time ti-1Between methane production (L)；Time tiWith time ti-1Positioned at measurement in 23 hours
In time, the serial number of the data acquisition in the minute that i is 23 hours；
RgFor the gas flow rate (L/h) of exhaust pipe；
CCiFor methane concentration (10 in exhaust pipe6L/L)；
CAiFor methane concentration (10 in open-air6L/L)；
VCFor breathing cabin product (m3)；
RiFor time tiWith time ti-1Between methane generating rate (L/h)；
The sum of data acquisition in the minute that n is 23 hours；
V23hFor the methane production in 23 hours minutes.
Step 4, the methane production V for 24 hours for calculating holstein cow24h, the methane in 23 hours minutes can be produced
Measure V23hWith the methane production V of missing 1h1hAddition obtain adduction calculated.Formula is as follows:
V24h=V23h+V1h
Lack the methane production V of 1h1hAveraging method can be used and close on value method and supplemented.
Averaging method calculates the methane production V of missing 1h1hUtilize following formula:
V1h=1 hour × V23h/ 23,
Closing on value method is the methane generating rate obtained using the last time measurement at the end of 23 hours minutes
As the methane generating rate of 1h before missing data, the methane production V of missing 1h is calculated1hUtilize following formula:
V1h=1 hour × Rmi
Wherein, RmiFor the R measured for the last time in 23 hours minutesi。
Influence (g/24h) of 2 calculation of table to milk cow discharge of methane
Test result: it is compared with the methane production for closing on the calculating of value method, the methane production that averaging method calculates significantly increases (table
2)。
Standard error refers to the standard error between two kinds of calculation method data.
Difference refers to (averaging method/close on value method -1).
P value is the significance analysis result of two kinds of calculation methods.
The result shows that: using the methane data for closing on value method supplement missing, it can more accurately calculate methane production.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention
The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method
In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.
Claims (3)
1. a kind of method cabin measurement using breathing and calculate milk cow gastrointestinal tract discharge of methane, which is characterized in that including following step
It is rapid:
Step 1: choosing more bovine cows, milk cow is caught up with into breathing cabin, every cow head is located at different breathing cabins, the basis feeding TMR
Daily ration, the interior feeding of continuous 23 hours minutes is twice；
Step 2 carries out Methane determination to each cow head respectively, in continuous 23 hours minutes, every setting measuring interval
Time measures the methane concentration of exhaust pipe discharge, and then the 1h time sweeps the health in breathing cabin；
Step 3 calculates time tiWith time ti-1Between methane production Δ Vi；Time tiWith time ti-1Positioned at survey in 23 hours
In fixing time, the serial number of the data acquisition in the minute that i is 23 hours,
△ViFor time tiWith time ti-1Between methane production (L)；Time tiWith time ti-1Positioned at 23 hours minutes
It is interior, the serial number of the data acquisition in the minute that i is 23 hours；
RgFor the gas flow rate of exhaust pipe, unit L/h；
CCiFor methane concentration in exhaust pipe, unit 106L/L；
CAiIt is 10 for methane concentration unit in open-air6L/L；
VCFor breathing cabin product, unit m3；
RiFor time tiWith time ti-1Between methane generating rate, unit L/h；
The sum of data acquisition in the minute that n is 23 hours；
V23hFor the methane production in 23 hours minutes.
2. a kind of method cabin measurement using breathing and calculate milk cow gastrointestinal tract discharge of methane according to claim 1,
It is characterized in that, further comprising the steps of:
Calculate the methane production V for 24 hours of milk cow24h:
V24h=V23h+V1h
V1h=1 hour × V23h/ 23 or V1h=1 hour × Rmi
Wherein, RmiFor the R measured for the last time in 23 hours minutesi。
3. a kind of method cabin measurement using breathing and calculate milk cow gastrointestinal tract discharge of methane according to claim 1,
It is characterized in that, the setting measuring interval time is less than or equal to 3 hours.
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| CA2725380A1 (en) * | 2008-05-23 | 2009-12-17 | Patrick R. Zimmerman | Method and system for monitoring and reducing ruminant methane production |
| CN202526176U (en) * | 2012-03-09 | 2012-11-14 | 华南农业大学 | Breathing box system for collecting methane of pig |
| CN102920458A (en) * | 2012-11-06 | 2013-02-13 | 杨华明 | Multifunctional open type respiratory metabolism testing device |
| CN104267127A (en) * | 2014-10-17 | 2015-01-07 | 中国科学院西北高原生物研究所 | Method for determining emission amount of greenhouse gas from ruminant livestock |
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Application publication date: 20190827 |