CN110170082A - A kind of circulating tumor cell is in body closed loop extract equipment - Google Patents
A kind of circulating tumor cell is in body closed loop extract equipment Download PDFInfo
- Publication number
- CN110170082A CN110170082A CN201910356560.3A CN201910356560A CN110170082A CN 110170082 A CN110170082 A CN 110170082A CN 201910356560 A CN201910356560 A CN 201910356560A CN 110170082 A CN110170082 A CN 110170082A
- Authority
- CN
- China
- Prior art keywords
- unit
- circulating tumor
- tumor cell
- connect
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 title claims abstract description 149
- 239000000284 extract Substances 0.000 title claims abstract description 57
- 210000004369 blood Anatomy 0.000 claims abstract description 115
- 239000008280 blood Substances 0.000 claims abstract description 115
- 238000003860 storage Methods 0.000 claims abstract description 74
- 239000004615 ingredient Substances 0.000 claims abstract description 66
- 238000010241 blood sampling Methods 0.000 claims abstract description 34
- 238000000605 extraction Methods 0.000 claims abstract description 21
- 239000011324 bead Substances 0.000 claims description 49
- 238000001514 detection method Methods 0.000 claims description 38
- 238000004891 communication Methods 0.000 claims description 35
- 238000000926 separation method Methods 0.000 claims description 30
- 239000012530 fluid Substances 0.000 claims description 21
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 5
- 238000013517 stratification Methods 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims description 3
- 230000009469 supplementation Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000004064 recycling Methods 0.000 claims 1
- 230000009885 systemic effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 description 23
- 238000011010 flushing procedure Methods 0.000 description 18
- 239000003146 anticoagulant agent Substances 0.000 description 16
- 229940127219 anticoagulant drug Drugs 0.000 description 15
- 230000005291 magnetic effect Effects 0.000 description 13
- 206010028980 Neoplasm Diseases 0.000 description 12
- 238000010586 diagram Methods 0.000 description 12
- 210000004881 tumor cell Anatomy 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 238000007599 discharging Methods 0.000 description 9
- 230000009471 action Effects 0.000 description 8
- 238000001727 in vivo Methods 0.000 description 8
- 238000013461 design Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 210000001616 monocyte Anatomy 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 230000005672 electromagnetic field Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 230000005389 magnetism Effects 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 125000002619 bicyclic group Chemical group 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000003211 malignant effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 125000003367 polycyclic group Chemical group 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 229940127090 anticoagulant agent Drugs 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000003302 ferromagnetic material Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/38—Removing constituents from donor blood and storing or returning remainder to body, e.g. for transfusion
Landscapes
- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Cardiology (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- External Artificial Organs (AREA)
Abstract
The invention discloses a kind of circulating tumor cells to be connect by the first blood transfusion tube with first separative unit in body closed loop extract equipment, including blood sampling unit, blood sampling unit；First separative unit is connect by the second blood transfusion tube with smart separative unit or storage unit, and first separative unit also passes through third blood transfusion tube and connect with feedback unit；First separative unit is for making blood be separated into the ingredient containing circulating tumor cell and the ingredient without circulating tumor cell；Smart separative unit is connect by the 4th blood transfusion tube with feedback unit, and smart separative unit also passes through delivery pipe and connect with storage unit；Smart separative unit is used to carry out the ingredient containing circulating tumor cell the extraction of circulating tumor cell；Storage unit is for storing ingredient and/or circulating tumor cell containing circulating tumor cell；Control unit is connect with blood sampling unit, first separative unit, smart separative unit, feedback unit respectively.Of the invention one, which has technical effect that, to filter out the circulating tumor cell in systemic blood.
Description
Technical field
The present invention relates to medical instruments fields, more particularly it relates to which a kind of circulating tumor cell is mentioned in body closed loop
Take equipment.
Background technique
Circulating tumor cell (circulating tumor cell), and it is called CTC for short.Circulating tumor cell is to exist
The general designation of all kinds of tumour cells in peripheral blood, because spontaneous or operation of diagnosis and treatment is from entity tumor lesion (primary tumor, transfer stove)
It falls off, most of circulating tumor cell occurs apoptosis or swallowed after entering peripheral blood, and minority can escape and develop into
Transfer stove increases malignant tumor patient mortality risk.As it can be seen that the recurrence and transfer of circulating tumor cell and malignant cell have
More close relationship.
Nowadays, medical technology is in continuous development and innovation, in order to further realize deeply grinding to circulating tumor cell
Study carefully, avoid the generation of malignant cell, the relevant instrument such as some detections to circulating tumor cell occur, extract or sets
It is standby.But these instruments or equipment contribute to carry out extracorporeal treatment to circulating tumor cell, that is, need first to extract a certain amount of
Blood, later in vitro to whether detecting in blood, and formulate corresponding therapeutic scheme containing circulating tumor cell etc..
In fact, the method for this extracorporeal treatment is typically only capable to extract a small amount of blood (about 10ml), and since circulating tumor is thin
Quantity of the born of the same parents in peripheral blood be usually it is fewer, generally require and sought in about more than one hundred million a leucocytes and several hundred hundred million red blood cells
Look for less amount of circulating tumor cell, it is possible to can generate and not follow in extracted sub-fraction blood (about 10ml)
Ring tumour cell, to cause false negative.It or is that circulating tumor is thin in the sub-fraction blood (about 10ml) of extraction
The content of born of the same parents is extremely low, such as: only one or several circulating tumor cells, then based on a series of originals such as detection sensitivities
Cause, it is also possible to the case where causing false negative.It can be seen that existing vitro detection circulating tumor cell very likely results in finally
As a result inaccurate, cause to be difficult to formulate suitable therapeutic scheme.
Summary of the invention
It is an object of the present invention to provide a kind of circulating tumor cell body closed loop extract equipment new solution.
According to an aspect of the invention, there is provided a kind of circulating tumor cell is in body closed loop extract equipment, including blood sampling
Unit, first separative unit, smart separative unit, storage unit, feedback unit and control unit；
The blood sampling unit is connect by the first blood transfusion tube with the just separative unit；
The just separative unit is connect by the second blood transfusion tube with the smart separative unit or storage unit, described just to divide
Also pass through third blood transfusion tube from unit to connect with the feedback unit；The just separative unit is configured as the effect in centrifugal force
Under so that blood is separated into the ingredient containing circulating tumor cell and the ingredient without circulating tumor cell；
The essence separative unit is connect by the 4th blood transfusion tube with the feedback unit, and the essence separative unit also passes through row
Pipe is put to connect with the storage unit；The essence separative unit is configurable for following the ingredient containing circulating tumor cell
The separation and Extraction of ring tumour cell；
The storage unit is configurable for storing ingredient and/or circulating tumor cell containing circulating tumor cell；
The feedback unit is configurable for the defeated time body of the ingredient without circulating tumor cell that will be isolated；
Described control unit passes through Communication Control line and the blood sampling unit respectively, first separative unit, smart separative unit, returns
Defeated unit connection.
Optionally, the outside of the blood sampling unit and feedback unit is respectively arranged with pressure sensor, and the pressure passes
Sensor passes through Communication Control line and connect with described control unit.
Optionally, the circulating tumor cell further includes component detection apparatus in body closed loop extract equipment；The ingredient
Detection device is connect by detection line or Communication Control line with the just separative unit, and the component detection apparatus also passes through inspection
Survey line or Communication Control line are connect with described control unit, and the component detection apparatus is configured as: in the control of control unit
System detects down the type for each ingredient isolated through first separative unit, purity and stratification state.
Optionally, the circulating tumor cell further includes ultrasonic bubble sensor in body closed loop extract equipment, described super
Sound air bubble sensor is connect with the feedback unit, and the ultrasonic bubble sensor also passes through Communication Control line and the control is single
Member connection.
Optionally, the circulating tumor cell further includes fluid replacing unit in body closed loop extract equipment, the fluid replacing unit
It is connect by liquid supplementation pipe with the feedback unit；The fluid replacing unit also passes through Communication Control line and connect with described control unit.
Optionally, the smart separative unit includes temporary storage location, and injection port, output are respectively arranged on the temporary storage location
Mouthful；
The injection port is configurable for the immunomagnetic beads that circulating tumor cell is extracted in the injection into temporary storage location；
The delivery outlet connects efferent duct, is provided with draw-off pump on the efferent duct, the end of the efferent duct is divided into two
A branch, a branch are connect with the 4th blood transfusion tube, another branch is connect with the delivery pipe, in the efferent duct
End is provided with switch, and the switch is connect by Communication Control line with described control unit, and the switch is configurable for
The on or off of two branches is controlled, and described two branches are configured as: being connected in one branch of same time, another
Branch shutdown；
It is provided on one section of tube wall of the efferent duct to recessed storage and stays slot, stayed in the storage and be provided with electricity outside slot
Magnetic cell, the electromagnetic unit are connect by Communication Control line with described control unit, immune after extracting circulating tumor cell
Immunomagnetic beads are attracted under the magneticaction of electromagnetic unit when flowing through storage and staying slot and are persisted to storage and stayed in slot by magnetic bead.
Optionally, the immunomagnetic beads outer surface is enclosed with circulating tumor cell immune antiboidy coating.
Optionally, the smart separative unit further includes pendulous device, and the temporary storage location is arranged on pendulous device.
Optionally, the smart separative unit further includes mesh plate, and the mesh plate is made of ferrimagnet, the net
Orifice plate is arranged in the efferent duct；
It is wound with electromagnetic coil in the corresponding output pipe outer wall of the mesh plate, the electromagnetic coil passes through Communication Control
Line is connect with described control unit；Either, permanent magnet is provided with outside the corresponding efferent duct of the mesh plate.
Optionally, the mesh plate is set as one or more；When multiple mesh plates are arranged, multiple mesh plates are in institute
State is in be parallel to each other and be spaced setting in efferent duct.
Circulating tumor cell provided in an embodiment of the present invention is in body closed loop extract equipment, with realizing closed loop to internal whole body
Blood carries out the inspection of circulating tumor cell, filters out, and not only can also can directly, continuously be filtered out complete with accurate detection
Circulating tumor cell contained in body blood, so as to be effectively prevented from recurrence and the transfer phenomena of malignant tumour.Moreover,
Due to the mode using systemic blood detection circulating tumor cell, avoids and be possible to detect in a small amount of blood or extract not
The case where to circulating tumor cell, it can avoid the false negative of testing result.
By referring to the drawings to the detailed description of exemplary embodiment of the present invention, other feature of the invention and its
Advantage will become apparent.
Detailed description of the invention
It is combined in the description and the attached drawing for constituting part of specification shows the embodiment of the present invention, and even
With its explanation together principle for explaining the present invention.
Fig. 1 is a kind of structural schematic diagram of the circulating tumor cell provided in an embodiment of the present invention in body closed loop extract equipment.
Fig. 2 is structural representation of another circulating tumor cell provided in an embodiment of the present invention in body closed loop extract equipment
Figure.
Fig. 3 is structural representation of another circulating tumor cell provided in an embodiment of the present invention in body closed loop extract equipment
Figure.
Fig. 4 is the structural schematic diagram of just separative unit provided in an embodiment of the present invention.
The first structural schematic diagram in centrifugation pond in Fig. 5 just separative unit provided in an embodiment of the present invention.
Second of structural schematic diagram in centrifugation pond in Fig. 6 just separative unit provided in an embodiment of the present invention.
The third structural schematic diagram in centrifugation pond in Fig. 7 just separative unit provided in an embodiment of the present invention.
The 4th kind of structural schematic diagram in centrifugation pond in Fig. 8 just separative unit provided in an embodiment of the present invention.
Fig. 9 is a kind of structural schematic diagram of smart separative unit provided in an embodiment of the present invention.
Figure 10 is the partial enlargement diagram of electromagnetic unit part in Fig. 9.
Figure 11 is the structural schematic diagram of another smart separative unit provided in an embodiment of the present invention.
Figure 12 is the schematic diagram of mesh plate absorption immunomagnetic beads provided in an embodiment of the present invention.
Figure 13 is that the embodiment of the present invention provides a kind of mesh plate set-up mode schematic diagram.
Figure 14 is the structural schematic diagram of another smart separative unit provided in an embodiment of the present invention.
Description of symbols:
1- blood sampling unit, just separative unit, 201- are centrifugated channel, 202- arrival end, the outlet end 203-, 204- half to 2-
Enclosed retaining groove, 205- connecting tube, 206- extraction mouth, 207- feed inlet, the first discharge port of 208-, the second discharge port of 209-,
210- is centrifuged pond, 211- third discharge port, 212- strainer, 213- feed pipe, 214- control switch, the 215- first branch, 216-
Second branch, 3- storage unit, 4- essence separative unit, 401- temporary storage location, 402- injection port, 403- efferent duct, 404- storage are stayed
Slot, 405- draw-off pump, 406- switch, 407- rocking equipment, 408- mesh plate, 4081- mesh, 409- inlet, 410- are immune
Magnetic bead, 411- electromagnetic coil, 5- feedback unit, 6- component detection apparatus, 7- anti-coagulants adding set, 8- pressure sensor, 9-
Delivery pipe, 10- electromagnetic unit, 11- control unit, 12- ultrasonic bubble sensor, 13- fluid replacing unit, the first blood transfusion tube of 14-,
The second blood transfusion tube of 15-, 16- third blood transfusion tube, the 4th blood transfusion tube of 17-, 18- discharging pipeline, the first control switch of 1801-, 19- punching
Washing machine structure, 1901- syringe pipe, 1902- flushing pipe, 1903- circulating pump, the second control switch of 1904-.
Specific embodiment
Carry out the various exemplary embodiments of detailed description of the present invention now with reference to attached drawing.It should also be noted that unless in addition having
Body explanation, the unlimited system of component and the positioned opposite of step, numerical expression and the numerical value otherwise illustrated in these embodiments is originally
The range of invention.
Be to the description only actually of at least one exemplary embodiment below it is illustrative, never as to the present invention
And its application or any restrictions used.
Technology, method and apparatus known to person of ordinary skill in the relevant may be not discussed in detail, but suitable
In the case of, the technology, method and apparatus should be considered as part of specification.
It is shown here and discuss all examples in, any occurrence should be construed as merely illustratively, without
It is as limitation.Therefore, other examples of exemplary embodiment can have different values.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, then in subsequent attached drawing does not need that it is further discussed.
Circulating tumor cell provided in an embodiment of the present invention can be used for the blood to entire patient in body closed loop extract equipment
It carries out the inspection of circulating tumor cell, filter out, so as to avoid the recurrence and transfer of malignant tumour.It should be noted that
In the present invention, it is described refer to " in body " be joined together to form one with body." closed loop " refers to entire blood treatment system
System forms a closed system with body, to avoid the intrusion such as external bacterium, avoids infection and causes danger.In addition, of the invention
The circulating tumor cell that embodiment provides can be applied to human body, animal body etc. in body closed loop extract equipment, and the present invention does not make this
Limitation.
Refering to what is shown in Fig. 1, circulating tumor cell provided in an embodiment of the present invention is included at least in body closed loop extract equipment:
Blood sampling unit 1, first separative unit 2, storage unit 3, smart separative unit 4, feedback unit 5 and control unit 11.
Wherein, blood sampling unit 1 may include the those skilled in the art such as blood taking needle, blood sampling pump, filter and heparin tube
Well known component, no longer illustrates herein.Specifically, blood sampling unit 1 can separate list with first by the first blood transfusion tube 14
Member 2 connects, at this point, the blood extracted from body can be sent to first point through the first blood transfusion tube 14 under the action of taking a blood sample pump
From in unit 2.That is, blood sampling unit 1 is used to extract blood from body and blood is sent into just separative unit 2.Also, this
It, can be by unit of taking a blood sample in order to realize the automation control run to blood sampling unit 1 (such as starting or stopping) in embodiment
1 is linked together by Communication Control line with control unit 11, is opened at this point it is possible to be realized by control unit 11 blood sampling unit 1
Control that is dynamic or stopping.
Wherein, with reference to shown in Fig. 1 and Fig. 2, first separative unit 2 by the second blood transfusion tube 15 and smart separative unit 4 or
Storage unit 3 connects, also, the first separative unit 2 is also connect by third blood transfusion tube 16 with feedback unit 5.The first separation is single
Member 2 is configured as: each ingredient in blood can be made to be separated into according to specific gravity containing circulating tumor cell under the influence of centrifugal force
Ingredient and ingredient without circulating tumor cell.At this point, the ingredient without circulating tumor cell can pass through third blood transfusion tube
16 are transmitted directly in feedback unit 5, are fed back in vivo by feedback unit 5, realize cyclic process.And for being separated from blood
Then there are two types of processing modes for the ingredient containing circulating tumor cell out: one of which is to be sent directly into storage unit 3 to deposit
Storage；There are also one is being sent into smart separative unit 4, the ingredient containing circulating tumor cell is carried out by smart separative unit 4 further
Separation and Extraction processing, to extract circulating tumor cell.Also, in order to realize the automation control to the operation of first separative unit 2
First separative unit 2 can be connect by Communication Control line with control unit 11, i.e., by control unit by system in the present embodiment
11 realize the control for running or stopping to first separative unit 2.
Wherein, smart separative unit 4 is configurable for extracting circulating tumor from the ingredient containing circulating tumor cell thin
Born of the same parents.Specifically, after first separative unit 2, blood is separated into ingredient containing circulating tumor cell and thin without circulating tumor
The ingredient of born of the same parents, in fact, current separation does not completely extract circulating tumor cell, what is isolated is containing circulating tumor
The ingredient of cell is also mixed with other monocyte ingredients in circulating tumor cell.Therefore, it devises in the present embodiment first
A smart separative unit 4 is added after separative unit 2, it is thin by immunomagnetic beads method to extract circulating tumor by smart separative unit 4
Born of the same parents, the circulating tumor cell extracted can be sent in storage unit 3 through delivery pipe 9 and be stored, and remaining be free of
The ingredient of circulating tumor cell is then sent in feedback unit 5, is fed back in vivo, is realized in body closed loop by the control of feedback unit 5
Cyclic process.The accurate extraction to the circulating tumor cell in blood may be implemented in this way.Due to have passed through separation and Extraction twice
Process can accurately extract circulating tumor cell from blood, be achieved that effective filter to circulating tumor cell
It removes.
Wherein, storage unit 3 is such as can be storage bag or storage pool with the device of store function, the present invention couple
This is with no restriction.In the present embodiment, storage unit 3 is using storage bag, and structure is simple, low manufacture cost, uses
It is convenient.
Wherein, feedback unit 5 may include the those skilled in the art such as blood transfusion needle, blood transfusion apparatus, filter and blood transfusion pipeline
Component known to member, no longer illustrates herein.Feedback unit 5 can be connect by the 4th blood transfusion tube 17 with smart separative unit 4,
To realize the connection of feedback unit 5 and smart separative unit 4.Certainly, it when without using smart separative unit 4, can also incite somebody to action
Feedback unit 5 is connect by third blood transfusion tube 16 with first separative unit 2, to realize the company of feedback unit 5 Yu first separative unit 2
It is logical.Feedback unit 5 is configured as: smart separative unit 4 can be made to isolate thin without circulating tumor under the action of feeding back pump
The ingredient of born of the same parents is directly sent back in vivo, or the ingredient without circulating tumor cell that first separative unit 2 is isolated is sent back in vivo.
Feedback unit 5 is matched with blood sampling unit 1 and be may be implemented in body closed loop cycle process.Also, in the present embodiment, in order to realize
To the automation control (such as starting or stop) that feedback unit 5 is run, can by feedback unit 5 by Communication Control line with
Control unit 11 connects, and realizes the control for starting to feedback unit 5 or stopping by control unit 11.
In the present embodiment, can be realized by control unit 11 to blood sampling unit 1, first separative unit 2, smart separative unit 4,
And the control respectively that feedback unit 5 works.Circulating tumor cell can be made to have in body closed loop extract equipment by the design
The characteristics of automation control.Optionally, control unit 11 can have display unit, and user can be by operating display unit
To control and observe the working condition etc. of each component.Wherein, display unit for example can be this fields such as touching display screen skill
Display device known to art personnel, the invention is not limited in this regard.
Circulating tumor cell provided in an embodiment of the present invention, can be to the circulation in systemic blood in body closed loop extract equipment
Tumour cell is detected and is filtered out.The circulating tumor cell is in body closed loop extract equipment using 2 (primary point of first separative unit
From extract) and essence separative unit 4 (secondary separation extraction) circulating tumor cell in blood is repeatedly detected and is filtered out, can
To completely remove the circulating tumor cell contained in blood, drawback existing in the prior art is effectively overcome.The circulating tumor
Cell has the characteristics that testing result is accurately and highly-safe in body closed loop extract equipment, can avoid the false yin of testing result
Property the case where, can be improved detection circulating tumor cell level.Moreover, the circulating tumor cell body closed loop extract equipment from
Dynamicization degree is high, time saving and energy saving, easy to operate.
In addition, refering to what is shown in Fig. 3, circulating tumor cell provided in an embodiment of the present invention may be used also in body closed loop extract equipment
To use single pin mode, i.e., blood sampling unit 1 is merged with feedback unit 5.At this point it is possible to be controlled by control unit 11
Blood sampling and feedback are alternately.Specifically: a needle can be used, which can serve as blood taking needle, can also act as blood transfusion
Needle.By the needle connecting line, blood will not be fed back to internal when from internal blood sampling, i.e. blood sampling is that timesharing carries out work with blood transfusion
Make, mutual interference phenomenon will not be generated.When blood was collected, blood sampling pump is controlled by control unit 11 and is operated,
Feedback pump is controlled simultaneously stops working (or small flow).When feeding back blood, blood sampling pump stalling is controlled by control unit 11 and (is stopped
Shi Liuliang is 0, is equivalent to pipeline closing, while controlling the operating of feedback pump).Wherein, take a blood sample pump flow be greater than feed back pump discharge
When, blood is flowed from needle to outflow to internal.It should be noted that for above-mentioned single pin mode, inside can only set
Just separative unit 2 is set, first separative unit 2 and smart separative unit 4 can also be cooperated use (as shown in Figure 3), the present invention
With no restriction to this.
In the present embodiment, the first blood transfusion tube 14, the second blood transfusion tube 15, third blood transfusion tube 16, the 4th blood transfusion tube 17 and row
It puts the pipelines such as pipe 9 and is all made of hose well known to those skilled in the art, in order to the conveying of blood.Also, these above-mentioned pipelines
Size can according to need and be adjusted flexibly, the invention is not limited in this regard.
With reference to shown in Fig. 1-Fig. 3, circulating tumor cell provided in an embodiment of the present invention, can be in body closed loop extract equipment
Including anti-coagulants adding set 7.Wherein, which is configured as: can add into blood sampling unit 1 anticoagulant
Agent.Specifically, when unit 1 of taking a blood sample, which extracts blood, to be sent into the first blood transfusion tube 14 and start to be recycled, pass through
Addition anti-coagulants can prevent the blood clotting handled, to can guarantee going on smoothly for entire blood circulation process.It needs
It is noted that the additive amount of anti-coagulants can according to need flexible control, the invention is not limited in this regard.
Wherein, anti-coagulants adding set 7 can be directly joined together with blood sampling unit 1.Certainly, anti-coagulants adding set
7 can also be connect by pipeline with blood sampling unit 1.The addition of anti-coagulants may be implemented in both modes, is specifically applying
In, it can flexible choice according to the actual situation.
Also, anti-coagulants adding set 7 can also be connect by Communication Control line with control unit 11.The design can be real
Existing control of the control unit 11 to anti-coagulants adding set 7, it can control anti-coagulants adding set 7 adds anticoagulant into blood
Agent, or stop adding anti-coagulants into blood.That is, can realize oneself add to anti-coagulants by control unit 11
Dynamic control, this mode operate more convenient, are also easy to control additive amount.Certainly, anti-coagulants adding set 7 can not also
It is connect with control unit 11, at this point it is possible to using the addition and additive amount that manually control anti-coagulants.It in a particular application can be with
Flexible choice as needed, the invention is not limited in this regard.
With reference to shown in Fig. 1-Fig. 3, circulating tumor cell provided in an embodiment of the present invention further includes in body closed loop extract equipment
Pressure sensor 8.Specifically, pressure sensor 8 is respectively arranged in the outside of blood sampling unit 1 and feedback unit 5.Pressure passes
Sensor 8 is connect by Communication Control line with control unit 11.And in order to realize accurate pressure detecting, pressure sensor 8 exists
It is intended to when setting close to corresponding blood transfusion tube.
Wherein, the pressure sensor 8 outside blood sampling unit 1 is arranged in adopt for detecting in blood sampling end, such as blood sampling unit
The pressure condition of blood vessel, and with the pressure condition in the first blood transfusion tube 14 for connecting of blood sampling unit 1.Similarly, setting is being returned
Pressure sensor 8 outside defeated unit 5 is used to detect the pressure condition in reinfusion end, such as feedback unit 5 in blood transfusion pipeline, with
And the pressure condition in the third blood transfusion tube 16 and the 4th blood transfusion tube 17 being connect with feedback unit 5.It can be sentenced by pressure detecting
Whether broken needle (such as blood taking needle, blood transfusion needle) and corresponding pipeline generate blocking.Once blocking, it is easy to cause pipe internal pressure
Power is abnormal, causes dangerous.
It should be noted that there are many setting positions of pressure sensor 8, however it is not limited to the mode in above-described embodiment.
Such as: by pressure sensor 8 be separately positioned on the first blood transfusion tube 14 and the second blood transfusion tube 15 on.Either, by pressure sensing
Device 8 is separately positioned in the first blood transfusion tube 14 and in the second blood transfusion tube 15.It can be adjusted flexibly according to the actual situation, the present invention couple
This is with no restriction.In addition, the setting quantity present invention for pressure sensor 8 is also not especially limited, spirit can according to need
It is living to select suitable quantity.
Also, each pressure sensor 8 can be connect by communication control line with control unit 11.The design can be real
Existing control of the control unit 11 to pressure sensor 8, it can control pressure sensing 8 starts pressure detecting on suitable opportunity,
Pressure sensor 8 can certainly be controlled without pressure detecting.
With reference to shown in Fig. 1-Fig. 3, circulating tumor cell provided in an embodiment of the present invention, can be in body closed loop extract equipment
Including component detection apparatus 6.Component detection apparatus 6 can be connect by detection line or Communication Control line with first separative unit 2,
The component detection apparatus 6 is also connect by detection line or Communication Control line with control unit 11.Component detection apparatus 6 is configured
Are as follows: detect the ingredient and purity of each section isolated through first separative unit 2 under control of the control unit 11, also, this at
Point detection device 6 can also control the ingredient separation process of first separative unit 2, so that different ingredients can be formed well
Stratification state, be conducive to extract different ingredients respectively.That is, blood is divided into not by separative unit 2 originally
With ingredient when, can detecte out stratification state, type and the purity of each ingredient by component detection apparatus 6, such as can be true
It is fixed whether to contain circulating tumor cell etc..
It should be noted that component detection apparatus 6 can also be connect by Communication Control line with smart separative unit 4, this hair
It is bright to this with no restriction.Also, component detection apparatus 6 can use detection device well known to those skilled in the art, herein not
It illustrates again.It is provided with or without in addition, component detection apparatus 6 can according to need selection, the present invention does not limit this
System.
With reference to shown in Fig. 1-Fig. 3, circulating tumor cell provided in an embodiment of the present invention further includes in body closed loop extract equipment
Ultrasonic bubble sensor 12, the ultrasonic bubble sensor 12 are located at one end where feedback unit 5.The ultrasonic bubble sensor 12
It is connect with feedback unit 5.Specifically, ultrasonic bubble sensor 12 is whether to contain gas in the blood fed back using ultrasound detection
Bubble, once detecting in blood will stop to internal feedback blood immediately and alarm containing bubble, otherwise blood enters body
In can cause danger.
Also, ultrasonic bubble sensor 12 is also connect by Communication Control line with control unit 11.The design may be implemented
Control of the control unit 11 to ultrasonic bubble sensor 12, it can control ultrasonic bubble sensor 12 starts on suitable opportunity
Detection, naturally it is also possible to control ultrasonic bubble sensor 12 without detection.That is, may be implemented by control unit 11
To the automation control of ultrasonic bubble sensor 12.
With reference to shown in Fig. 1-Fig. 3, circulating tumor cell provided in an embodiment of the present invention, can be in body closed loop extract equipment
Including fluid replacing unit 13.The fluid replacing unit 13 is configured as: when feeding back blood, the interior carry out fluid infusion into blood, such as can be with
To internal Additional nutrient solution etc..Wherein, fluid replacing unit 13 can be connect by liquid supplementation pipe with feedback unit 5.Certainly, fluid replacing unit
13 can also be combined together with feedback unit 5.The two ways may be implemented to add nutrition into the blood of feedback
Liquid or medical fluid.
Also, fluid replacing unit 13 can be connect by Communication Control line with control unit 11.The design may be implemented to control
Control of the unit 11 to fluid replacing unit 13, to realize the automation of fluid infusion.
It should be noted that the solidifying agent adding set 7 and fluid replacing unit 13 in the present invention can use dedicated pump, fortune
Row stops to be controlled by control unit 11.Pressure sensor 8, ultrasonic bubble sensor 12 in the present invention, at
Point detection device 6 is also controlled by control unit 11, and the automation of operation is realized.
For circulating tumor cell provided in an embodiment of the present invention in body closed loop extract equipment, just separative unit 2 therein is main
It is that separating treatment is carried out to blood based on centrifuge separation principle, blood is separated into different ingredients, such as without containing circulation
The ingredient of tumour cell and the ingredient containing circulating tumor cell etc..
Refering to what is shown in Fig. 4, provided in an embodiment of the present invention, just separative unit 2, structure can be with are as follows: logical including being centrifugated
Road 201, the centrifuge separation channel 201 are respectively provided with arrival end 202, outlet end 203.Wherein, arrival end 202 is configured as: being used
In the raw material to be separated such as input blood into centrifuge separation channel 201.Centrifuge separation channel 201 is configured as: can be around wherein
The heart carries out the rotation of predetermined speed, and generates centrifugal force by rotation, for making the ingredient of different specific weight in blood in centrifugal force
Under the action of generate separation, it is different at layering to be formed.It is respectively provided with corresponding to each at the position of layering on outlet end 203
Have and extracts mouth 206 (such as the quantity of extraction mouth 206 can according to need flexible setting, and this is not restricted by the present invention), it is each
Extract mouth 206 be respectively used to output it is current at layering.Centrifuge separation channel 201 close to outlet end 203 one section of size be
It gradually broadens, and the wall surface of this section forms involute structure, which is conducive to improve separation accuracy.
Wherein, centrifuge separation channel 201 can be monocycle road structure, or bicyclic road structure can also be polycyclic
Road structure, specifically can flexible choice according to the actual situation.It should be noted that the bicyclic road structure is not meant that completely
Two coil structures of closure, it is only necessary to more than a circle, such as the structure of a circle half.Certainly, the polycyclic road structure is also such as
This, does not mean that many coil structures being closed completely.
Optionally, Semi surrounding type retaining groove 204 is formed at extraction mouth 206 with reference to shown in Fig. 4.The Semi surrounding type retaining groove
204 are configured as: from extract mouth 206 take out it is current at layering before, for enabling ingredient to be first gathered together, and
It prevents from other being mixed at the ingredient of layering.The design is very suitable to the extraction for content rareness ingredient, such as extracts in blood
Circulating tumor cell.
With reference to Fig. 4 and Fig. 5, above-mentioned first separative unit 2 can also include centrifugation pond 210, the centrifugation pond 210 difference
With feed end and discharge end.Strainer 212 can be set in the centrifugation pond 210, and strainer 212 is extended to from feed end
Discharge end.It is provided with feed inlet 207 on feed end, at least one discharge port (such as first in Fig. 4 is provided on discharge end
Discharge port 208 and the second discharge port 209, either, the first discharge port 208, the second discharge port 209 and third discharging in Fig. 5
Mouth is 211).Wherein, the feed inlet 207 for being centrifuged pond 210 and some for being centrifugated channel 201 are extracted can lead between mouth 206
Cross the connection of connecting tube 205.Wherein, centrifugation pond 210 is configured as: when centrifuge separation channel 201 is rotated, being centrifuged pond 210
Can be rotated together with centrifuge separation channel 201, for from extract ingredient that mouth 206 takes out again, centrifuge separation, such as into one
Step separates and extracts circulating tumor cell from blood, and other ingredients is avoided to be mixed into circulating tumor cell as far as possible.
Wherein, one or more can be set in strainer 212 in centrifugation pond 210.It is multiple when being arranged in centrifugation pond 210
When strainer 212, the mesh size of strainer 212 be may be the same or different.The size of feed inlet 207 can according to need spirit
Adjustment living.Quantity and size of discharge port etc., which also can according to need, to be adjusted flexibly.The present invention to this with no restriction.
In fact, since the usual content of circulating tumor cell is relatively low (belonging to rare cell in blood), into
After row centrifuge separation, the position in centrifugation layering is close with other monocytes, is very easy to be mixed at the extraction other
Monocyte.In order to the good circulating tumor cell separated and extracted in blood, first separative unit 2 of the invention is used
Centrifuge separation channel 201 and it is centrifuged the mode that pond 210 cooperates, is also not necessarily limited to the structure in the present invention certainly.
In the specific embodiment of the present invention, refering to what is shown in Fig. 5, first by centrifuge separation channel 201 to blood
Liquid is centrifuged, and the ingredient containing circulating tumor cell can be separated under the influence of centrifugal force, and converge in
In Semi surrounding type retaining groove 204, it can be exported later by corresponding extraction mouth 206, then the company by being connected with the extraction mouth 206
Ingredient containing circulating tumor cell is introduced into centrifugation pond 210 by adapter tube 205.Wherein, in centrifugation pond 210 there are two settings
Strainer 212, the two strainers 212 can be in being oppositely arranged, and the aperture size of the two strainers 212 can be designed as difference, and two
The space being centrifuged in pond 210 can be separated into three regions by a strainer 212, and feed inlet 207 can be located at two strainers 212 and enclose
At region in.At this point, being centrifuged the ingredient containing circulating tumor cell that channel 201 is isolated is sent directly into two filters
In the region that net 212 surrounds, under the influence of centrifugal force, a strainer 212 can pass through for light and small blood plasma and blood platelet,
And extract by the first discharge port 208, another strainer 212 can pass through for heavier and small in size red blood cell, and by the
Two discharge ports 209 extract, and the biggish circulating tumor cell of volume and leucocyte etc. can be deposited in two strainers 212 and enclose
At region in, can be extracted by the way that third discharge port 211 in the area is arranged.At this point, that isolates contains
The ingredient of circulating tumor cell can be sent out through third discharge port 211, and the feed pipe 213 by connecting with third discharge port 211
It is conveyed.
Feed pipe 213 provided in an embodiment of the present invention, end can be divided into two branches and (such as be divided into and be denoted as first
Road 215, second branch 216), and the position on end branch road is provided with control switch 214.Wherein, the first branch 215 can be with
It is connected to feedback unit 5 and (feedback unit 5 can be connected if without circulating tumor cell).And second branch 216 can with deposit
Storage unit 3 or smart separative unit 4 are connected to, and above-mentioned control switch 214 can be connected by Communication Control line and control unit 11
It connects, i.e., under control of the control unit 11, feed pipe 213 can be connected with any one branch therein.Specifically: working as control
Switch 214 controls conveying pipeline 213 and the first branch 215 and realizes when being connected, it may be possible to do not extract follow tumour cell (if
There is no circulating tumor cell in blood) or do not have to extract, at this time can directly by accordingly at being distributed into the 4th blood transfusion tube 17, and
It is sent back in vivo by feedback unit 5.When control switch 214 controls conveying pipeline 213 and second branch 216 is connected, that isolates contains
The ingredient of circulating tumor cell is sent in storage unit 3 and is stored, and either, the ingredient containing circulating tumor cell passes through the
Two blood transfusion tubes 15 are admitted in smart separative unit 4, to carry out the extraction of further circulating tumor cell.
Refering to what is shown in Fig. 6, can also include flushing machine 19 in first separative unit 2 of the invention.The flushing machine 19
Including syringe pipe 1901, syringe pipe 1901 connects flushing pipe 1902, and circulating pump 1903 is provided in flushing pipe 1902.It rinses
Mechanism 19 can be used for rinsing the strainer 212 in centrifugation pond 210.Specifically, when on filter screen 212 attachment it is more, obstruction filter
When mesh on net 212, syringe pipe 1901 can be used, strainer 212 is rinsed；Also, terminate in circulating tumor cell extraction
When strainer 212 can also be rinsed, to prevent retained cyclical tumour cell on strainer 212.
Optionally, syringe pipe 1901 can be set in the side of strainer 212, can also be all provided in the two sides of strainer 212
Syringe pipe 1901 is set, the surrounding of strainer 212 can be made to be distributed syringe pipe 1901 at this time, to realize to strainer 212 without dead angle
It rinses.It should be noted that flushing pipe 1902 can supply water when being rinsed, cleaning solution can also be supplied, herein no longer
It illustrates.
Refering to what is shown in Fig. 7, centrifugation pond 210 provided in an embodiment of the present invention, can also be not provided with strainer 212 inside it,
The width dimensions of discharge end can be increased under this structure, i.e. from feed end to discharge end, width is gradually to become in centrifugation pond 210
Big (showing discharge end with reference to Fig. 7).After blood, which is centrifuged channel 201, to be centrifugated, containing circulating tumor cell
Part, which is separated, (in fact, not only containing circulating tumor cell in the ingredient isolated at this time, it is thin also to contain circulating tumor
A variety of cells other than born of the same parents), and be introduced in centrifugation pond 210 and be centrifugated again.Through being centrifuged in centrifugation pond 210
Afterwards, heavy cell can be assembled outward, and can extract by the second discharge port 209, and the cell of light specific gravity can be to
Inside aggregation, and can extract by the first discharge port 208.In order to determine the cell component that is extracted from each discharge port and
Purity can be detected using component detection apparatus 6.Specifically:
Discharge outlet (being in the first discharge port 208 shown in a Fig. 7) connection discharging pipeline 18 wherein, at sorting
Surveying device 6 can be set in the outside of the discharging pipeline 18, for detecting to the ingredient conveyed in discharging pipeline 18, with true
It makes the ingredient conveyed in expects pipe road 18 and has suffered type, purity etc..The end of the discharging pipeline 18 can also be divided into two branches
(be divided into and be denoted as the first branch, second branch), and the position on the end branch road of discharging pipeline 18 is provided with the first control and opens
1801 are closed, the first control switch 1801 is connect by Communication Control line with control unit 11, it can by control unit 11 to this
First control switch 1801 is controlled.By the first control switch 1801, leading for the first branch or second branch may be implemented
It is logical.Wherein, the first branch can be connected to storage unit 3 or smart separative unit 4, and second branch can connect with feedback unit 5
It is logical.That is, first can be passed through when component detection apparatus 6 detects that the ingredient in discharging pipeline 18 contains circulating tumor cell
Branch its be sent to storage unit 3 or smart separative unit 4 (carry out continuing to sort to circulating tumor cell).Work as composition detection
When device 6 detects the ingredient in discharging pipeline 18 without circulating tumor cell, feedback can will be delivered to by second branch
Unit 5, it is final to feed back in vivo.
It should be noted that the spectral absorption characteristics that component detection apparatus 6 can use circulating tumor cell are detected
Or detected using the spectral absorption characteristics of monocyte, it no longer illustrates herein.Component detection apparatus 6 can reflect
Sensor or transmission sensor.
Refering to what is shown in Fig. 8, can be connected in another discharge outlet above-mentioned on the basis of Fig. 7 shows centrifugation pond 210
Flushing machine 19, in order to be rinsed to centrifugation pond 210.
It should be noted that the discharge port in connection flushing machine 19 will not generally export circulating tumor cell or containing following
The ingredient of ring tumour cell.Specifically: the syringe pipe 1901 of the flushing machine 19 connects with the discharge port, and syringe pipe 1901 connects
Flushing pipe 1902 is connect, circulating pump 1903 is set in flushing pipe 1902.Also, it is divided into two branch in the end of flushing pipe 1902
Road is provided with the second control switch 1904 in the position on the end branch road of flushing pipe 1902, and the second control switch 1904 passes through
Communication Control line is connect with control unit 11, it can is controlled by control unit 11 second control switch 1904.It is logical
Cross the second control switch 1904 may be implemented two branches conducting or shutdown (but in the one branch conducting of same time,
The shutdown of another branch), i.e., a branch can be connected to feedback unit 5, and the ingredient for that will be free of circulating tumor cell is defeated
In ex vivo, another branch is off at this time.After the components back that will be free of circulating tumor cell is complete, another branch quilt
Conducting, can inject the water and/or cleaning solution of flushing into flushing pipe 1902, can be used for rushing centrifugation pond 210 at this time
Wash processing.In the same time, two branches do not interact.
Wherein, for first separative unit 2, flushing machine 19 can according to need selection and be provided with or without, if
Seated position can also be adjusted flexibly, the invention is not limited in this regard.
In addition, it is necessary to explanation, just separative unit provided in an embodiment of the present invention has diversified forms, however it is not limited on
The form stated.Such as the centrifuge separation before being centrifuged pond 210 can be using Centrifuge Cup, the centrifugation this fields such as bag and centrifugal pan skill
Centrifugation apparatus known to art personnel, the invention is not limited in this regard.
Refering to what is shown in Fig. 9, in the present invention, junior's separation screening unit by smart separative unit 4 as first separative unit 2,
The ingredient containing circulating tumor cell for isolating from blood continues precisely to separate, in order to realize to circulating tumor
The purification processes of cell, and remaining ingredient without circulating tumor cell is transmitted back in vivo by feedback unit 5.
Refering to what is shown in Fig. 9, smart separative unit 4 of the invention, may include temporary storage location 401, on the temporary storage location 401
It is respectively arranged with injection port 402, delivery outlet.After blood is centrifuged in first separative unit 2, containing circulating tumor cell
Temporarily stored in the temporary storage location 401 that ingredient can be transported in smart separative unit 4 through the second blood transfusion tube 15, later again into
The subsequent processings such as the extraction of row circulating tumor cell, to realize the separation and Extraction to circulating tumor cell.
Wherein, temporary storage location 401 can have the device of store function, the present invention couple using storage bag or storage pool etc.
This is with no restriction.Also, for the amount of storage of storage unit 401, the present invention with no restriction, can also carry out according to the actual situation
Adjustment.
Wherein, injection port 402 is configured as: the ingredient containing circulating tumor cell through the output of first separative unit 2 enters temporary
After memory cell 401, especially after reaching predetermined amount, extraction can be injected into temporary storage location 402 by the injection port 402 and followed
The immunomagnetic beads 410 of ring tumour cell.Such purpose is to enable immunomagnetic beads adequately and containing circulating tumor cell
Ingredient contact.
Wherein, immunomagnetic beads 410 belong to super-paramagnetic bead, are resisted on immunomagnetic beads 410 by the immune of respective cycle tumour cell
Body is coated with (such as coating, i.e., antibody is coated in the surface of immunomagnetic beads).When circulating tumor cell encounters immunomagnetic beads 410
It will be attracted capture, immunomagnetic beads 410 can show good magnetism in magnetic field.Wherein, the injection rate of immunomagnetic beads 410
It can be adjusted flexibly according to the actual situation, the invention is not limited in this regard.
Also, refering to what is shown in Fig. 9, efferent duct 403 is connected on the delivery outlet of temporary storage location 401, on efferent duct 403
It is provided with draw-off pump 405.And the end of efferent duct 403 is divided into two branches, one of branch is connect with the 4th blood transfusion tube 17,
For realizing the connection with feedback unit 5, another branch is connect with delivery pipe 9, for realizing the connection with storage unit 3.
Moreover, being additionally provided with switch 406 in the position on the end branch road of efferent duct 403, which passes through Communication Control line and control
Unit 11 processed connects.Switch 406 is configurable for the on or off of two branches of control, wherein two branches are configured
Are as follows: in the one branch conducting of same time, another branch shutdown, i.e., in the same time, when control efferent duct 403 and a branch
When road is connected to, what another branch was off, vice versa.
Also, slot 404 is stayed refering to what is shown in Fig. 9, being provided on one section of efferent duct 403 of tube wall to recessed storage.It should
It can be for example rectangle or semicircular arc etc., the invention is not limited in this regard that the longitudinal section form of slot 404 is stayed in storage.Of the invention
Storage stays slot 404 to be extracted the immunomagnetic beads 410 of circulating tumor cell for retaining.
The outside of slot 404 is stayed to be provided with electromagnetic unit 10 in storage.Electromagnetic unit 10 passes through Communication Control line and control unit
11 connections, it can electromagnetic unit 10 is controlled by control unit 11.The fluid flowed into efferent duct 403 is stayed flowing through storage
When slot 404, electromagnetic unit 10 can produce electromagnetic field, and the immunomagnetic beads of circulating tumor cell will be captured under the action of electromagnetic field
410, which are attracted to storage, stays in slot 404, is extracted by immunomagnetic beads method to circulating tumor cell with realizing, avoids magnetic bead defeated
In ex vivo.That is, immunomagnetic beads 410 can be used for sorption cycle tumour cell, temporary storage location under the action of draw-off pump 405
What the fluid in 401 can continue is flowed into efferent duct 403, after the starting of electromagnetic unit 10, when immunomagnetic beads 410 enter electromagnetism
In the electromagnetic field that unit 10 generates, immunomagnetic beads 410 will be attracted, and be then deposited in storage and stayed slot 404.Due to being immunized before
Magnetic bead 410 is combined with circulating tumor cell, and such circulating tumor cell has also just been left on storage and has stayed in slot 404, at this time
By switch 406, the components back unit 5 not extracted by immunomagnetic beads 410 can be made.And when the fluid in temporary storage location 401
When being extracted, another branch can be connected by switch 406, and close electromagnetic unit 10, storage can be made to stay in slot 404
Immunomagnetic beads 410 are delivered in storage unit 3 under the action of draw-off pump 405.Immunomagnetic beads in storage unit 3 can be taken
It is further detected after out.
It should be noted that if being free of circulating tumor cell in blood, then blood enters essence separation list through first separative unit 2
After in member 4, by adjusting switch 406, blood can be sent directly into feedback unit 5 through branch by efferent duct 403, feed back internal.
Wherein, in order to realize the attraction to immunomagnetic beads 410, other than using electromagnetic unit 10, slot can also be stayed in storage
Permanent magnets are arranged in 404 outsides, but when using permanent magnet, need manually storage stay placed outside slot 404 permanent magnet or
Person removes permanent magnet, is more troublesome.Electromagnetic unit 10 is used in the present invention, it, can be with by the connection with control unit 11
Realize the automatic control to electromagnetic unit 10.
In fact, refering to what is shown in Fig. 10, the immunomagnetic beads 410 in injection temporary storage location 401 can to capture circulating tumor thin
Born of the same parents, to realize the extraction to circulating tumor cell, and immunomagnetic beads 410 can show good magnetism in magnetic field, can be along magnetic
The line of force is mobile to electromagnetic unit 10, is sucked, fixes by electromagnetic unit 10, and other ingredients are not due to having corresponding antibodies, will not
It is captured by immunomagnetic beads 410, can be transferred under the action of draw-off pump 405 with proper flow, can enter feedback unit 5, and
It feeds back internal.
With reference to shown in Figure 11, it can also include pendulous device 407 that the present invention, which implements the smart separative unit 4 provided,.It is temporary single
Member 401 can be set on pendulous device 407.After starting pendulous device 407, pendulous device 407 can drive temporary storage location 401
It shakes back and forth, the abundant combination of internal immunomagnetic beads 410 and circulating tumor cell can be promoted in this way.Wherein, pendulous device
407 are mainly used for realizing the shaking back and forth of temporary storage location 401, can use equipment well known to those skilled in the art, such as put
Moving frame no longer illustrates herein.
With reference to shown in Figure 11, essence separative unit 4 provided in an embodiment of the present invention, further includes mesh plate 408.Mesh plate 408
It is arranged in efferent duct 403.Mesh 4081 on the mesh plate 408 can be the shapes such as round or rectangular.
Wherein, mesh plate 408 is made of magnetic material, such as ferromagnetic material.Also, it is corresponding defeated in mesh plate 408
Electromagnetic coil 411 is wound on the outer wall of outlet pipe 403.Magnetic field can be formed after the energization of electromagnetic coil 411.Specifically, electric
Magnetic coil 411 can be connect by Communication Control line with control unit 11.Based on the superparamagnetism of immunomagnetic beads 410, Ke Yi
Good magnetism is shown in magnetic field.When the immunomagnetic beads 410 that carry circulating tumor cell encounter mesh plate 408, it is immunized
Magnetic bead 410 can be adsorbed on mesh plate 408.Due to having adsorbed circulating tumor cell before immunomagnetic beads 410, then recycle
Tumour cell can also be attracted on mesh plate 408, and other ingredients can pass through mesh plate 408, and through efferent duct 403
It is delivered to feedback unit 5, is finally fed back in vivo.With reference to shown in Figure 12, when immunomagnetic beads 410 have been attracted to mesh plate 408,
Immunomagnetic beads 410 are usually fixed in the side of mesh 4081.
In addition, permanent magnet can also be used in the outside of the corresponding efferent duct 403 of mesh plate 408.Permanent magnet can also
To generate magnetic field, when immunomagnetic beads 410 pass through, immunomagnetic beads 410 can also be made to be adsorbed on mesh plate 408.
Wherein, mesh plate 408 can be set one, naturally it is also possible to be arranged multiple.It is multiple when being arranged in efferent duct 403
It when mesh plate 408, can be arranged by the way of being parallel to each other between different mesh plates 408, and two adjacent mesh plates
There is interval between 408.For example, mesh plate 408 can be set to two with reference to shown in Figure 13, the two mesh plates 408 are defeated
It is in be arranged parallel and there is therebetween interval in outlet pipe 403.It should be noted that more when being arranged in efferent duct 403
When a mesh plate 408, different its aperture size of mesh plate 408 be may be the same or different, can be according to practical application feelings
Condition is adjusted flexibly.Also, mesh plate 408 should be arranged along the cross section of efferent duct 403, to avoid mesh plate 408 and efferent duct
Gap is formed between 403 inner walls.Mesh plate 408 should be stayed with storage has appropriately distance between slot 404.
In addition, being additionally provided at least one inlet at mesh plate 408 on efferent duct 403 with reference to shown in Figure 14
409.The inlet 409 can be used for injecting immunomagnetic beads 410 or corresponding drug.Since inlet 409 is apart from mesh plate 408
Closer, once immunomagnetic beads 410 enter efferent duct 403, mesh plate 408 can easily adsorb immunomagnetic beads 410, when temporary
When raw material to be separated in memory cell 401 passes through mesh plate 408, the immunomagnetic beads 410 adsorbed on mesh plate 408 can be further
Capture circulating tumor cell.When not starting pendulous device 407, immunomagnetic beads 410 can be also injected into using from inlet 409
Mode, in order to sufficiently extract circulating tumor cell.
With reference to shown in Figure 14, smart separative unit 4 of the invention also may include flushing machine 19 above-mentioned.Herein, it rinses
Mechanism 19 can be used for being rinsed the attachment on mesh plate 408.Such as: when attachment blocks mesh more on mesh plate 408
When, it needs to be rinsed mesh plate 408；Certainly, when circulating tumor cell extract at the end of, can also to mesh plate 408 into
Row rinses, and prevents circulating tumor cell from remaining on mesh plate 408.
Wherein, syringe pipe 1901 can be provided only on any side of mesh plate 408.It is of course also possible in mesh plate 408
Two sides be respectively provided with syringe pipe 1901 so that syringe pipe 1901 can be evenly distributed on the surrounding of mesh plate 408, which is conducive to
Realize the abundant flushing to mesh plate 408.
It should be noted that using control unit 11 to entire circulating tumor cell in body closed loop extract equipment in the present invention
Interior all parts are controlled, such as various pumps, various switches etc., to realize the automation of whole equipment.In addition, Fig. 1-figure
In 14, each pump, switch and branch line position can change, move, such as: no matter pump is preceding or rear
Can Fluid Motion Driven By Moving, the present invention to this with no restriction.
Although some specific embodiments of the invention are described in detail by example, the skill of this field
Art personnel it should be understood that example above merely to being illustrated, the range being not intended to be limiting of the invention.The skill of this field
Art personnel are it should be understood that can without departing from the scope and spirit of the present invention modify to above embodiments.This hair
Bright range is defined by the following claims.
Claims (10)
1. a kind of circulating tumor cell is in body closed loop extract equipment, which is characterized in that including the unit, first separative unit, essence of taking a blood sample
Separative unit, storage unit, feedback unit and control unit；
The blood sampling unit is connect by the first blood transfusion tube with the just separative unit；
The just separative unit is connect by the second blood transfusion tube with the smart separative unit or storage unit, and the just separation is single
Member is also connect by third blood transfusion tube with the feedback unit；The just separative unit is configured as making under the influence of centrifugal force
Blood is separated into the ingredient containing circulating tumor cell and the ingredient without circulating tumor cell；
The essence separative unit is connect by the 4th blood transfusion tube with the feedback unit, and the essence separative unit also passes through delivery pipe
It is connect with the storage unit；The essence separative unit is configurable for carrying out recycling to the ingredient containing circulating tumor cell swollen
The separation and Extraction of oncocyte；
The storage unit is configurable for storing ingredient and/or circulating tumor cell containing circulating tumor cell；
The feedback unit is configurable for the defeated time body of the ingredient without circulating tumor cell that will be isolated；
Described control unit passes through Communication Control line and the blood sampling unit respectively, first separative unit, smart separative unit, feeds back list
Member connection.
2. circulating tumor cell according to claim 1 is in body closed loop extract equipment, which is characterized in that the blood sampling unit
It is respectively arranged with pressure sensor with the outside of feedback unit, and the pressure sensor passes through Communication Control line and the control
Unit connection processed.
3. circulating tumor cell according to claim 1 is in body closed loop extract equipment, which is characterized in that further include into sorting
Survey device；
The component detection apparatus is connect by detection line or Communication Control line with the just separative unit, the composition detection
Device also passes through detection line or Communication Control line is connect with described control unit, and the component detection apparatus is configured as:
The type for each ingredient isolated through first separative unit, purity and stratification state are detected under the control of control unit.
4. circulating tumor cell according to claim 1 is in body closed loop extract equipment, which is characterized in that further include ultrasonic gas
Sensor is steeped, the ultrasonic bubble sensor is connect with the feedback unit, and the ultrasonic bubble sensor also passes through communication control
Line processed is connect with described control unit.
5. circulating tumor cell according to claim 1 is in body closed loop extract equipment, which is characterized in that further include fluid infusion list
Member, the fluid replacing unit are connect by liquid supplementation pipe with the feedback unit；The fluid replacing unit also passes through Communication Control line and institute
State control unit connection.
6. circulating tumor cell according to claim 1 is in body closed loop extract equipment, which is characterized in that the essence separation is single
Member includes temporary storage location, is respectively arranged with injection port, delivery outlet on the temporary storage location；
The injection port is configurable for the immunomagnetic beads that circulating tumor cell is extracted in the injection into temporary storage location；
The delivery outlet connects efferent duct, is provided with draw-off pump on the efferent duct, the end of the efferent duct is divided into two branch
Road, a branch are connect with the 4th blood transfusion tube, another branch is connect with the delivery pipe, in the end of the efferent duct
It is provided with switch, the switch is connect by Communication Control line with described control unit, and the switch is configurable for controlling
The on or off of two branches, and described two branches are configured as: in the one branch conducting of same time, another branch
Shutdown；
It is provided on one section of tube wall of the efferent duct to recessed storage and stays slot, stayed in the storage and be provided with electromagnetism list outside slot
Member, the electromagnetic unit are connect by Communication Control line with described control unit, the immunomagnetic beads after extracting circulating tumor cell
When flowing through storage and staying slot, immunomagnetic beads are attracted under the magneticaction of electromagnetic unit and are persisted to storage and are stayed in slot.
7. circulating tumor cell according to claim 6 is in body closed loop extract equipment, which is characterized in that the immunomagnetic beads
Outer surface is enclosed with circulating tumor cell immune antiboidy coating.
8. circulating tumor cell according to claim 6 is in body closed loop extract equipment, which is characterized in that the essence separation is single
Member further includes pendulous device, and the temporary storage location is arranged on pendulous device.
9. circulating tumor cell according to claim 6 is in body closed loop extract equipment, which is characterized in that the essence separation is single
Member further includes mesh plate, and the mesh plate is made of ferrimagnet, and the mesh plate is arranged in the efferent duct；
Be wound with electromagnetic coil in the corresponding output pipe outer wall of the mesh plate, the electromagnetic coil by Communication Control line with
Described control unit connection；Either,
Permanent magnet is provided with outside the corresponding efferent duct of the mesh plate.
10. circulating tumor cell according to claim 9 is in body closed loop extract equipment, which is characterized in that the mesh plate
It is set as one or more；When multiple mesh plates are arranged, multiple mesh plates in the efferent duct in be parallel to each other and
Every setting.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910356560.3A CN110170082A (en) | 2019-04-29 | 2019-04-29 | A kind of circulating tumor cell is in body closed loop extract equipment |
| PCT/CN2019/124725 WO2020220684A1 (en) | 2019-04-29 | 2019-12-12 | In-vivo closed-loop extraction device for circulating tumor cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910356560.3A CN110170082A (en) | 2019-04-29 | 2019-04-29 | A kind of circulating tumor cell is in body closed loop extract equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110170082A true CN110170082A (en) | 2019-08-27 |
Family
ID=67690273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910356560.3A Pending CN110170082A (en) | 2019-04-29 | 2019-04-29 | A kind of circulating tumor cell is in body closed loop extract equipment |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN110170082A (en) |
| WO (1) | WO2020220684A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020192183A1 (en) * | 2019-03-27 | 2020-10-01 | 刘忠英 | Component separation device |
| WO2020220684A1 (en) * | 2019-04-29 | 2020-11-05 | 刘忠英 | In-vivo closed-loop extraction device for circulating tumor cells |
| CN114813302A (en) * | 2022-06-01 | 2022-07-29 | 华中科技大学同济医学院附属协和医院 | Multiple circulating tumor cell single-sampling and automatic identification machine |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102271729A (en) * | 2008-12-23 | 2011-12-07 | 特拉科斯有限公司 | Processing blood |
| CN204275126U (en) * | 2014-10-28 | 2015-04-22 | 北京麦邦光电仪器有限公司 | High precision closed loop blood constituent is separated blood-taking device in real time |
| CN107922910A (en) * | 2016-07-29 | 2018-04-17 | 苏文弘 | Microfluidic device and application thereof and application method |
| CN108977404A (en) * | 2018-07-14 | 2018-12-11 | 北京航空航天大学青岛研究院 | Sizing screening method based on immunomagnetic beads label cell |
| CN210331253U (en) * | 2019-04-29 | 2020-04-17 | 刘忠英 | Circulating tumor cell in-vivo closed-loop extraction equipment |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10127068A1 (en) * | 2001-05-23 | 2002-11-28 | Bio Medical Apherese Systeme G | Apparatus for the separation of leukocytes, from blood, has a number of stations to separate marked components with magnetic particles without any adverse effects on the blood |
| CN2776411Y (en) * | 2005-03-18 | 2006-05-03 | 缪文良 | Circulation blood immune target treating device |
| WO2012119646A1 (en) * | 2011-03-08 | 2012-09-13 | Kentsch Michael | Method for reducing the risk of metastasis formation in cancerous diseases, and system for removing tumour cells from the blood circulation |
| CN105062874A (en) * | 2015-07-16 | 2015-11-18 | 南京大学医学院附属鼓楼医院 | Circulating tumor cell separating and enriching device on basis of closed loops |
| CN110170082A (en) * | 2019-04-29 | 2019-08-27 | 刘忠英 | A kind of circulating tumor cell is in body closed loop extract equipment |
-
2019
- 2019-04-29 CN CN201910356560.3A patent/CN110170082A/en active Pending
- 2019-12-12 WO PCT/CN2019/124725 patent/WO2020220684A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102271729A (en) * | 2008-12-23 | 2011-12-07 | 特拉科斯有限公司 | Processing blood |
| CN204275126U (en) * | 2014-10-28 | 2015-04-22 | 北京麦邦光电仪器有限公司 | High precision closed loop blood constituent is separated blood-taking device in real time |
| CN107922910A (en) * | 2016-07-29 | 2018-04-17 | 苏文弘 | Microfluidic device and application thereof and application method |
| CN108977404A (en) * | 2018-07-14 | 2018-12-11 | 北京航空航天大学青岛研究院 | Sizing screening method based on immunomagnetic beads label cell |
| CN210331253U (en) * | 2019-04-29 | 2020-04-17 | 刘忠英 | Circulating tumor cell in-vivo closed-loop extraction equipment |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020192183A1 (en) * | 2019-03-27 | 2020-10-01 | 刘忠英 | Component separation device |
| WO2020220684A1 (en) * | 2019-04-29 | 2020-11-05 | 刘忠英 | In-vivo closed-loop extraction device for circulating tumor cells |
| CN114813302A (en) * | 2022-06-01 | 2022-07-29 | 华中科技大学同济医学院附属协和医院 | Multiple circulating tumor cell single-sampling and automatic identification machine |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020220684A1 (en) | 2020-11-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110170082A (en) | A kind of circulating tumor cell is in body closed loop extract equipment | |
| JP3712182B2 (en) | Method for obtaining intermediate density component blood products with increased yield | |
| US4223672A (en) | Variable volume plasma treatment chamber for an apparatus for the extracorporeal treatment of disease | |
| CN102271729B (en) | Processing blood | |
| JP2930419B2 (en) | Red blood cell plasmapheresis apparatus and method | |
| JP5723026B2 (en) | System and method for collecting platelets and predicting return of plasma | |
| TW515720B (en) | Platelet collecting apparatus | |
| CN106267422B (en) | Rh blood group incompatibility haemolysis disease therapeutic apparatus | |
| US11850347B2 (en) | Systems and methods for automated recovery of white blood cells after producing a leuko-reduced blood product | |
| US10251990B2 (en) | System and method for processing, incubating, and/or selecting biological cells | |
| CN210331253U (en) | Circulating tumor cell in-vivo closed-loop extraction equipment | |
| CN104711188A (en) | Device for separating tumor cells in blood fluid | |
| US10274495B2 (en) | System and method for separating cells incorporating magnetic separation | |
| JP2015536719A (en) | System and method for continuous separation of whole blood | |
| CN107746794A (en) | A kind of cell separation apparatus | |
| CN2776411Y (en) | Circulation blood immune target treating device | |
| CN108865654A (en) | A kind of cell sorting device and method for separating | |
| CN212308926U (en) | Platelet-rich plasma preparation device | |
| CN106267407B (en) | Female tire Rh blood group incompatibility blood purifying therapeutical instrument | |
| CN210176835U (en) | Fine extraction element of circulation tumor cell | |
| JP4485380B2 (en) | Blood purification equipment | |
| CN106267405B (en) | Female tire blood group incompatibility haemolysis disease therapeutic apparatus | |
| CN204550582U (en) | The device of tumour cell in a kind of separating blood fluid | |
| CN209238165U (en) | A kind of Beads enrichment mechanism, device | |
| CN106267421B (en) | Female tire blood group incompatibility plasma purification device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |