CN110169357A - A kind of production method of rice paddy seed - Google Patents
A kind of production method of rice paddy seed Download PDFInfo
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- CN110169357A CN110169357A CN201910529142.XA CN201910529142A CN110169357A CN 110169357 A CN110169357 A CN 110169357A CN 201910529142 A CN201910529142 A CN 201910529142A CN 110169357 A CN110169357 A CN 110169357A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of production methods of rice paddy seed, comprising the following steps: S100: seed screening；Good stress resistance is selected, fringe bigger than normal, eutrophy, commodity is good, the excellent paddy rice cross breeding seed of superior rice and suitable rice seedling on upland field；S200: calli induction；S300: callus is sprouted；S400: culture of rootage；S500: nutrition is cultivated.The present invention produces more rice paddy seeds by the way that rice paddy seed is carried out dedifferentiation and is broken up again, it can not only guarantee that the merit of rice paddy seed is not destroyed, and the production time of rice paddy seed is short, the productivity of rice paddy seed is high, and the nutritive value of rice is improved, can effectively improve rice to the adaptability of soil.
Description
Technical field
The present invention relates to field of agricultural production technologies, more particularly to a kind of production method of rice paddy seed.
Background technique
China agricultural resource occupancy volume per person is very low, and per capita cultivated land's occupancy volume is only the one third of world average level,
With the quickening of urbanization process, the trend successively decreased year by year is presented in China's cultivated area, existing with the research and development of various hybrid rice
Rice is substantially hybrid rice in the market, and hybrid rice has disease-resistant resistance strong, and stable yields and high yield etc. are a series of
Advantage has widely been promoted the use of in China.
However, existing hybrid rice, which cannot reserve seed for planting, carries out production next time, at the same existing hybrid rice battalion
It forms point single.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of production method of rice paddy seed, using following technical side
Case is realized:
A kind of production method of rice paddy seed, comprising the following steps:
S100: seed screening；
Good stress resistance is selected, fringe bigger than normal, eutrophy, commodity is good, and the excellent rice of superior rice and suitable rice seedling on upland field is miscellaneous
Hand over seed；
S200: calli induction；
It is 0.6%- that excellent paddy rice cross breeding seed, which is peelled off ethyl alcohol and mass fraction that kind of skin is 75% with mass fraction,
0.8% liquor potassic permanganate is dried up after successively sterilizing using sterile water wash, and the excellent paddy rice cross breeding seed of peeling is accessed
In induced medium, it is placed in 27 DEG C of incubators and is inverted culture, obtain Rice Callus；
S300: callus is sprouted；
Rice Callus is clamped sizeable one section using tweezers and pliers, and it is gently put into training of sprouting
It supports in base, 25-35 DEG C of culture 48h；
S400: culture of rootage；
Rice Callus is taken out from culture medium of sprouting and is transferred in root media together with rice bud and is trained
It supports, is transferred in MS solid medium after 25-35 DEG C of culture 48h and continues to cultivate, after rice root and rice bud grow turn
Move on to plantation Tanaka's plantation, to rice it is mature after harvest rice paddy seed；
S500: nutrition is cultivated；
Rice paddy seed is mixed with the zeins of its weight 6-8%, be added to 47-50 times of mixture weight,
In the ethanol solution of 85-90%, Heat preservation 20-30 minutes at 24-30 DEG C, discharging washes seed 3-4 times, and discharging is dry
It is dry, it is mixed with the selenium-rich nutritive inorganic agent of its weight 4-6%, seed dressing is uniform.
Further, in s 200, the constituent of the induced medium is potassium nitrate 4.0g/L, ammonium nitrate 3.0g/
L, potassium dihydrogen phosphate 0.24g/L, magnesium sulfate 0.15g/L, calcium chloride 3.8g/L, sucrose 40g/L, agar 8g/L, potassium iodide
0.53mg/L, boric acid 8.2mg/L, manganese sulfate 36.3mg/L, zinc sulfate 6.8mg/L, sodium sulphate 0.45mg/L, copper sulphate
0.07mg/L, cobalt chloride 00.01mg/L, disodium ethylene diamine tetraacetate 45.2mg/L, ferrous sulfate 30.8mg/L, inositol 130mg/
L, glycine 4mg/L, thiamine hydrochloride 0.8mg/L, puridoxine hydrochloride 0.23mg/L, niacin 0.56mg/L, metakentrin
200.0mg/L, agar 2%, surplus are distilled water.
Further, in S300, the constituent of the culture medium of sprouting is potassium nitrate 2.4g/L, ammonium nitrate 1.7g/
L, potassium dihydrogen phosphate 0.36g/L, magnesium sulfate 0.24g/L, calcium chloride 3.6g/L, sucrose 45g/L, agar 8g/L, potassium iodide
0.67mg/L, boric acid 5.8mg/L, manganese sulfate 32.3mg/L, inositol 120mg/L, glycine 3mg/L, thiamine hydrochloride 0.2mg/
L, puridoxine hydrochloride 0.4mg/L, niacin 0.2mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, indoles
Acetic acid 2.2mg/L, plant cytokinin 3.0mg/L, agar 2%, surplus are distilled water.
Further, in S400, the root media constituent is manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/
L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, sulphur
Sour ferrous iron 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, cigarette
Sour 0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, heteroauxin 2.2mg/L, plant cell point
Plain 1.8mg/L, agar 2% are split, surplus is distilled water.
Further, in S500, what the selenium-rich nutritive inorganic agent was made of the raw material of following weight parts: pine needle
Powder 6-9, myristic acid 1.3-2, sophorolipid 0.4-1, yeast selenium 0.1-0.2, lauric acid amide of ethanol 0.1-0.3, cortex albiziae
The hydrogen peroxide of 2-3, pearl white 5-7, ethylmaltol 0.3-0.5, ferrous sulfate 0.1-0.2, rose dry petal 4-6,5-7%
10-14。
Preferably, the selenium-rich nutritive inorganic agent preparation method the following steps are included:
(1) above-mentioned pine needle meal is added in the sodium hydroxide solution of 10-13mol/l, the heat-insulation soaking 1- at 57-60 DEG C
Precipitating washing 2-3 times is mixed with above-mentioned myristic acid, is added to the deionized water of 20-30 times of mixture weight by 2h, filtering
In, increase temperature be 50-60 DEG C, insulated and stirred 15-20 minutes, the hydrogen peroxide of above-mentioned 5-7% is added dropwise, stirred after being added dropwise to
Room temperature must aoxidize pine needle meal solution；
(2) above-mentioned ethylmaltol, ferrous sulfate are mixed, are added in the deionized water of 57-60 times of mixture weight,
Insulated and stirred 10-17 minutes at 57-60 DEG C, maltol ferrous solution is obtained；
(3) above-mentioned sophorolipid is added in 20-30 times of its weight, 70-76% ethanol solution, increasing temperature is
60-64 DEG C, insulated and stirred 20-30 minutes, above-mentioned yeast selenium is added, stirs to room temperature, mixes, stir with above-mentioned maltol ferrous solution
It mixes uniformly, above-mentioned lauric acid amide of ethanol is added, it is 3-5 minutes ultrasonic, obtain amide dispersion liquid；
(4) above-mentioned oxidation pine needle meal solution, amide dispersion liquid are mixed, is stirred evenly, remaining each raw material is added, stirring is equal
It is even, it is spray-dried to get the selenium-rich nutritive inorganic agent.
The utility model has the advantages that
The present invention produces more rice paddy seeds by the way that rice paddy seed is carried out dedifferentiation and is broken up again, can not only protect
The merit of card rice paddy seed is not destroyed, and the production time of rice paddy seed is short, and the productivity of rice paddy seed is high；And
, rice paddy seed of the invention is dressed seed using selenium-rich nutritive inorganic agent, which by pine needle meal oxidation processes, improves first
Then the benefit materials such as selenium, iron are distributed in oxidation pine needle meal system by amide dispersion liquid, can be played very by biocidal property
Good slow release effect, the absorption into rice to nutriment improve the nutritive value of finished product, and inorganic agent of the invention can make
It obtains seed normally to germinate, make agriculture chemical slow release, inorganic agent of the invention provides selenium abundant, improves the battalion of rice
Value is supported, wherein being also added into amide dispersion liquid, can effectively improve rice to the adaptability of soil.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to specific reality of the invention
The mode of applying elaborates.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But this
Invention can be implemented with being much different from other way described herein, and those skilled in the art can be without prejudice to the present invention
Similar improvement is done in the case where intension, therefore the present invention is not limited by following public specific embodiment.
Below in conjunction with embodiment, the invention will be further described.
Specific embodiment 1
A kind of production method of the rice paddy seed of the present embodiment, comprising the following steps:
S100: seed screening；
Good stress resistance is selected, fringe bigger than normal, eutrophy, commodity is good, and the excellent rice of superior rice and suitable rice seedling on upland field is miscellaneous
Hand over seed；
S200: calli induction；
It is 0.6%- that excellent paddy rice cross breeding seed, which is peelled off ethyl alcohol and mass fraction that kind of skin is 75% with mass fraction,
0.8% liquor potassic permanganate is dried up after successively sterilizing using sterile water wash, and the excellent paddy rice cross breeding seed of peeling is accessed
In induced medium, it is placed in 27 DEG C of incubators and is inverted culture, obtain Rice Callus；
S300: callus is sprouted；
Rice Callus is clamped sizeable one section using tweezers and pliers, and it is gently put into training of sprouting
It supports in base, 25 DEG C of culture 48h；
S400: culture of rootage；
Rice Callus is taken out from culture medium of sprouting and is transferred in root media together with rice bud and is trained
It supports, is transferred in MS solid medium after 25 DEG C of culture 48h and continues to cultivate, be transferred to after rice root and rice bud grow
Plant Tanaka's plantation, to rice it is mature after harvest rice paddy seed；
S500: nutrition is cultivated；
Rice paddy seed mixed with the zeins of its weight 6%, be added to 47 times of mixture weight, 85%
In ethanol solution, Heat preservation 20 minutes at 24 DEG C, discharging washes seed 3 times, and discharge drying, with its weight 4-6%'s
The mixing of selenium-rich nutritive inorganic agent, seed dressing are uniform.
Further, in s 200, the constituent of the induced medium is potassium nitrate 4.0g/L, ammonium nitrate 3.0g/
L, potassium dihydrogen phosphate 0.24g/L, magnesium sulfate 0.15g/L, calcium chloride 3.8g/L, sucrose 40g/L, agar 8g/L, potassium iodide
0.53mg/L, boric acid 8.2mg/L, manganese sulfate 36.3mg/L, zinc sulfate 6.8mg/L, sodium sulphate 0.45mg/L, copper sulphate
0.07mg/L, cobalt chloride 00.01mg/L, disodium ethylene diamine tetraacetate 45.2mg/L, ferrous sulfate 30.8mg/L, inositol 130mg/
L, glycine 4mg/L, thiamine hydrochloride 0.8mg/L, puridoxine hydrochloride 0.23mg/L, niacin 0.56mg/L, metakentrin
200.0mg/L, agar 2%, surplus are distilled water.
Further, in S300, the constituent of the culture medium of sprouting is potassium nitrate 2.4g/L, ammonium nitrate 1.7g/
L, potassium dihydrogen phosphate 0.36g/L, magnesium sulfate 0.24g/L, calcium chloride 3.6g/L, sucrose 45g/L, agar 8g/L, potassium iodide
0.67mg/L, boric acid 5.8mg/L, manganese sulfate 32.3mg/L, inositol 120mg/L, glycine 3mg/L, thiamine hydrochloride 0.2mg/
L, puridoxine hydrochloride 0.4mg/L, niacin 0.2mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, indoles
Acetic acid 2.2mg/L, plant cytokinin 3.0mg/L, agar 2%, surplus are distilled water.
Further, in S400, the root media constituent is manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/
L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, sulphur
Sour ferrous iron 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, cigarette
Sour 0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, heteroauxin 2.2mg/L, plant cell point
Plain 1.8mg/L, agar 2% are split, surplus is distilled water.
Further, in S500, what the selenium-rich nutritive inorganic agent was made of the raw material of following weight parts: pine needle
Powder 6-9, myristic acid 1.3, sophorolipid 0.4, yeast selenium 0.1, lauric acid amide of ethanol 0.1, cortex albiziae 2, pearl white 5, second
Base maltol 0.3, ferrous sulfate 0.1, rose dry petal 4,5% hydrogen peroxide 10.
Preferably, the selenium-rich nutritive inorganic agent preparation method the following steps are included:
(1) above-mentioned pine needle meal is added in the sodium hydroxide solution of 10mol/l, the heat-insulation soaking 1h at 57 DEG C, is filtered,
It by precipitating washing 2 times, mixes, is added in 20 times of mixture weight of deionized water with above-mentioned myristic acid, increasing temperature is
50 DEG C, insulated and stirred 15 minutes, above-mentioned 5% hydrogen peroxide is added dropwise, it is molten must to aoxidize pine needle meal to room temperature for stirring after being added dropwise
Liquid；
(2) above-mentioned ethylmaltol, ferrous sulfate are mixed, is added in 57 times of mixture weight of deionized water,
Insulated and stirred 10 minutes at 57 DEG C, obtain maltol ferrous solution；
(3) above-mentioned sophorolipid is added in 20 times of its weight, 70% ethanol solution, increasing temperature is 60 DEG C, is protected
Temperature stirring 20 minutes, is added above-mentioned yeast selenium, stirs to room temperature, mixes, stir evenly, in addition with above-mentioned maltol ferrous solution
Lauric acid amide of ethanol is stated, ultrasound 3 minutes obtains amide dispersion liquid；
(4) above-mentioned oxidation pine needle meal solution, amide dispersion liquid are mixed, is stirred evenly, remaining each raw material is added, stirring is equal
It is even, it is spray-dried to get the selenium-rich nutritive inorganic agent.
Specific embodiment 2
A kind of production method of the rice paddy seed of the present embodiment, comprising the following steps:
S100: seed screening；
Good stress resistance is selected, fringe bigger than normal, eutrophy, commodity is good, and the excellent rice of superior rice and suitable rice seedling on upland field is miscellaneous
Hand over seed；
S200: calli induction；
It is 0.6%- that excellent paddy rice cross breeding seed, which is peelled off ethyl alcohol and mass fraction that kind of skin is 75% with mass fraction,
0.8% liquor potassic permanganate is dried up after successively sterilizing using sterile water wash, and the excellent paddy rice cross breeding seed of peeling is accessed
In induced medium, it is placed in 27 DEG C of incubators and is inverted culture, obtain Rice Callus；
S300: callus is sprouted；
Rice Callus is clamped sizeable one section using tweezers and pliers, and it is gently put into training of sprouting
It supports in base, 35 DEG C of culture 48h；
S400: culture of rootage；
Rice Callus is taken out from culture medium of sprouting and is transferred in root media together with rice bud and is trained
It supports, is transferred in MS solid medium after 35 DEG C of culture 48h and continues to cultivate, be transferred to after rice root and rice bud grow
Plant Tanaka's plantation, to rice it is mature after harvest rice paddy seed；
S500: nutrition is cultivated；
Rice paddy seed mixed with the zeins of its weight 8%, be added to 50 times of mixture weight, 90%
In ethanol solution, Heat preservation 30 minutes at 30 DEG C, discharging washes seed 4 times, and discharge drying, with its weight 4-6%'s
The mixing of selenium-rich nutritive inorganic agent, seed dressing are uniform.
Further, in s 200, the constituent of the induced medium is potassium nitrate 4.0g/L, ammonium nitrate 3.0g/
L, potassium dihydrogen phosphate 0.24g/L, magnesium sulfate 0.15g/L, calcium chloride 3.8g/L, sucrose 40g/L, agar 8g/L, potassium iodide
0.53mg/L, boric acid 8.2mg/L, manganese sulfate 36.3mg/L, zinc sulfate 6.8mg/L, sodium sulphate 0.45mg/L, copper sulphate
0.07mg/L, cobalt chloride 00.01mg/L, disodium ethylene diamine tetraacetate 45.2mg/L, ferrous sulfate 30.8mg/L, inositol 130mg/
L, glycine 4mg/L, thiamine hydrochloride 0.8mg/L, puridoxine hydrochloride 0.23mg/L, niacin 0.56mg/L, metakentrin
200.0mg/L, agar 2%, surplus are distilled water.
Further, in S300, the constituent of the culture medium of sprouting is potassium nitrate 2.4g/L, ammonium nitrate 1.7g/
L, potassium dihydrogen phosphate 0.36g/L, magnesium sulfate 0.24g/L, calcium chloride 3.6g/L, sucrose 45g/L, agar 8g/L, potassium iodide
0.67mg/L, boric acid 5.8mg/L, manganese sulfate 32.3mg/L, inositol 120mg/L, glycine 3mg/L, thiamine hydrochloride 0.2mg/
L, puridoxine hydrochloride 0.4mg/L, niacin 0.2mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, indoles
Acetic acid 2.2mg/L, plant cytokinin 3.0mg/L, agar 2%, surplus are distilled water.
Further, in S400, the root media constituent is manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/
L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, sulphur
Sour ferrous iron 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, cigarette
Sour 0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, heteroauxin 2.2mg/L, plant cell point
Plain 1.8mg/L, agar 2% are split, surplus is distilled water.
Further, in S500, what the selenium-rich nutritive inorganic agent was made of the raw material of following weight parts: pine needle
Powder 9, myristic acid 2, sophorolipid 1, yeast selenium 0.2, lauric acid amide of ethanol 0.3, cortex albiziae 3, pearl white 7, ethyl malt
Phenol 0.5, ferrous sulfate 0.2, rose dry petal 6,7% hydrogen peroxide 14.
Preferably, the selenium-rich nutritive inorganic agent preparation method the following steps are included:
(1) above-mentioned pine needle meal is added in the sodium hydroxide solution of 13mol/l, the heat-insulation soaking 2h at 60 DEG C, is filtered,
It by precipitating washing 3 times, mixes, is added in 30 times of mixture weight of deionized water with above-mentioned myristic acid, increasing temperature is
60 DEG C, insulated and stirred 20 minutes, above-mentioned 7% hydrogen peroxide is added dropwise, it is molten must to aoxidize pine needle meal to room temperature for stirring after being added dropwise
Liquid；
(2) above-mentioned ethylmaltol, ferrous sulfate are mixed, is added in 60 times of mixture weight of deionized water,
Insulated and stirred 10-17 minutes at 60 DEG C, maltol ferrous solution is obtained；
(3) above-mentioned sophorolipid is added in 30 times of its weight, 76% ethanol solution, increasing temperature is 64 DEG C, is protected
Temperature stirring 30 minutes, is added above-mentioned yeast selenium, stirs to room temperature, mixes, stir evenly, in addition with above-mentioned maltol ferrous solution
Lauric acid amide of ethanol is stated, ultrasound 5 minutes obtains amide dispersion liquid；
(4) above-mentioned oxidation pine needle meal solution, amide dispersion liquid are mixed, is stirred evenly, remaining each raw material is added, stirring is equal
It is even, it is spray-dried to get the selenium-rich nutritive inorganic agent.
Specific embodiment 3
A kind of production method of the rice paddy seed of the present embodiment, comprising the following steps:
S100: seed screening；
Good stress resistance is selected, fringe bigger than normal, eutrophy, commodity is good, and the excellent rice of superior rice and suitable rice seedling on upland field is miscellaneous
Hand over seed；
S200: calli induction；
It is 0.6%- that excellent paddy rice cross breeding seed, which is peelled off ethyl alcohol and mass fraction that kind of skin is 75% with mass fraction,
0.8% liquor potassic permanganate is dried up after successively sterilizing using sterile water wash, and the excellent paddy rice cross breeding seed of peeling is accessed
In induced medium, it is placed in 27 DEG C of incubators and is inverted culture, obtain Rice Callus；
S300: callus is sprouted；
Rice Callus is clamped sizeable one section using tweezers and pliers, and it is gently put into training of sprouting
It supports in base, 32 DEG C of culture 48h；
S400: culture of rootage；
Rice Callus is taken out from culture medium of sprouting and is transferred in root media together with rice bud and is trained
It supports, is transferred in MS solid medium after 32 DEG C of culture 48h and continues to cultivate, be transferred to after rice root and rice bud grow
Plant Tanaka's plantation, to rice it is mature after harvest rice paddy seed；
S500: nutrition is cultivated；
Rice paddy seed mixed with the zeins of its weight 7%, be added to 48 times of mixture weight, 87%
In ethanol solution, Heat preservation 25 minutes at 28 DEG C, discharging washes seed 3 times, and discharge drying, the richness with its weight 5%
The mixing of selemium nutrition inorganic agent, seed dressing are uniform.
Further, in s 200, the constituent of the induced medium is potassium nitrate 4.0g/L, ammonium nitrate 3.0g/
L, potassium dihydrogen phosphate 0.24g/L, magnesium sulfate 0.15g/L, calcium chloride 3.8g/L, sucrose 40g/L, agar 8g/L, potassium iodide
0.53mg/L, boric acid 8.2mg/L, manganese sulfate 36.3mg/L, zinc sulfate 6.8mg/L, sodium sulphate 0.45mg/L, copper sulphate
0.07mg/L, cobalt chloride 00.01mg/L, disodium ethylene diamine tetraacetate 45.2mg/L, ferrous sulfate 30.8mg/L, inositol 130mg/
L, glycine 4mg/L, thiamine hydrochloride 0.8mg/L, puridoxine hydrochloride 0.23mg/L, niacin 0.56mg/L, metakentrin
200.0mg/L, agar 2%, surplus are distilled water.
Further, in S300, the constituent of the culture medium of sprouting is potassium nitrate 2.4g/L, ammonium nitrate 1.7g/
L, potassium dihydrogen phosphate 0.36g/L, magnesium sulfate 0.24g/L, calcium chloride 3.6g/L, sucrose 45g/L, agar 8g/L, potassium iodide
0.67mg/L, boric acid 5.8mg/L, manganese sulfate 32.3mg/L, inositol 120mg/L, glycine 3mg/L, thiamine hydrochloride 0.2mg/
L, puridoxine hydrochloride 0.4mg/L, niacin 0.2mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, indoles
Acetic acid 2.2mg/L, plant cytokinin 3.0mg/L, agar 2%, surplus are distilled water.
Further, in S400, the root media constituent is manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/
L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, sulphur
Sour ferrous iron 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, cigarette
Sour 0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, heteroauxin 2.2mg/L, plant cell point
Plain 1.8mg/L, agar 2% are split, surplus is distilled water.
Further, in S500, what the selenium-rich nutritive inorganic agent was made of the raw material of following weight parts: pine needle
Powder 8, myristic acid 1.7, sophorolipid 0.8, yeast selenium 0.15, lauric acid amide of ethanol 0.2, cortex albiziae 2.5, pearl white 6, second
Base maltol 0.4, ferrous sulfate 0.15, rose dry petal 5,6% hydrogen peroxide 12.
Preferably, the selenium-rich nutritive inorganic agent preparation method the following steps are included:
(1) above-mentioned pine needle meal is added in the sodium hydroxide solution of 12mol/l, heat-insulation soaking 1.5h, mistake at 58 DEG C
Precipitating washing 2 times is mixed with above-mentioned myristic acid, is added in 25 times of mixture weight of deionized water by filter, increases temperature
It is 55 DEG C, insulated and stirred 17 minutes, above-mentioned 6% hydrogen peroxide is added dropwise, it is molten must to aoxidize pine needle meal to room temperature for stirring after being added dropwise
Liquid；
(2) above-mentioned ethylmaltol, ferrous sulfate are mixed, is added in 58 times of mixture weight of deionized water,
Insulated and stirred 14 minutes at 58 DEG C, obtain maltol ferrous solution；
(3) above-mentioned sophorolipid is added in 25 times of its weight, 73% ethanol solution, increasing temperature is 62 DEG C, is protected
Temperature stirring 25 minutes, is added above-mentioned yeast selenium, stirs to room temperature, mixes, stir evenly, in addition with above-mentioned maltol ferrous solution
Lauric acid amide of ethanol is stated, ultrasound 4 minutes obtains amide dispersion liquid；
(4) above-mentioned oxidation pine needle meal solution, amide dispersion liquid are mixed, is stirred evenly, remaining each raw material is added, stirring is equal
It is even, it is spray-dried to get the selenium-rich nutritive inorganic agent.
Although hereinbefore having been made with reference to some embodiments, present invention is described, of the invention not departing from
In the case where range, various improvement can be carried out to it and can be with equivalent without replacement technical point therein, as long as being not present
Technical contradiction, the various features in the various embodiments of institute's careless mistake of the present invention can be used in combination with by either type,
The description for not carrying out exhaustion in the present invention to the case where these combinations, only in the considerations of omitting length and economizing on resources.
Therefore, the invention is not limited to specific embodiments disclosed herein, and including falling into claim.
Claims (6)
1. a kind of production method of rice paddy seed, which comprises the following steps:
S100: seed screening；
Good stress resistance is selected, fringe bigger than normal, eutrophy, commodity is good, the excellent hybrid rice seed of superior rice and suitable rice seedling on upland field
Son；
S200: calli induction；
It is 0.6%-0.8%'s that excellent paddy rice cross breeding seed, which is peelled off ethyl alcohol and mass fraction that kind of skin is 75% with mass fraction,
Liquor potassic permanganate is dried up after successively sterilizing using sterile water wash, and the excellent paddy rice cross breeding seed of peeling is accessed Fiber differentiation
In base, it is placed in 27 DEG C of incubators and is inverted culture, obtain Rice Callus；
S300: callus is sprouted；
Rice Callus is clamped sizeable one section using tweezers and pliers, and it is gently put into culture medium of sprouting
In, 25-35 DEG C of culture 48h；
S400: culture of rootage；
Rice Callus is taken out from culture medium of sprouting and is transferred in root media together with rice bud and is cultivated,
It is transferred in MS solid medium after 25-35 DEG C of culture 48h and continues to cultivate, be transferred to after rice root and rice bud grow
Plant Tanaka's plantation, to rice it is mature after harvest rice paddy seed；
S500: nutrition is cultivated；
Rice paddy seed is mixed with the zeins of its weight 6-8%, is added to 47-50 times of mixture weight, 85-
In 90% ethanol solution, Heat preservation 20-30 minutes at 24-30 DEG C, discharging washes seed 3-4 times, and discharge drying,
It is mixed with the selenium-rich nutritive inorganic agent of its weight 4-6%, seed dressing is uniform.
2. a kind of production method of rice paddy seed according to claim 1, which is characterized in that in s 200, the induction
The constituent of culture medium be potassium nitrate 4.0g/L, ammonium nitrate 3.0g/L, potassium dihydrogen phosphate 0.24g/L, magnesium sulfate 0.15g/L,
Calcium chloride 3.8g/L, sucrose 40g/L, agar 8g/L, potassium iodide 0.53mg/L, boric acid 8.2mg/L, manganese sulfate 36.3mg/L, sulphur
Sour zinc 6.8mg/L, sodium sulphate 0.45mg/L, copper sulphate 0.07mg/L, cobalt chloride 00.01mg/L, disodium ethylene diamine tetraacetate
45.2mg/L, ferrous sulfate 30.8mg/L, inositol 130mg/L, glycine 4mg/L, thiamine hydrochloride 0.8mg/L, hydrochloric acid pyrrole are trembled
Alcohol 0.23mg/L, niacin 0.56mg/L, metakentrin 200.0mg/L, agar 2%, surplus are distilled water.
3. a kind of production method of rice paddy seed according to claim 1, which is characterized in that described to sprout in S300
The constituent of culture medium be potassium nitrate 2.4g/L, ammonium nitrate 1.7g/L, potassium dihydrogen phosphate 0.36g/L, magnesium sulfate 0.24g/L,
Calcium chloride 3.6g/L, sucrose 45g/L, agar 8g/L, potassium iodide 0.67mg/L, boric acid 5.8mg/L, manganese sulfate 32.3mg/L, flesh
Alcohol 120mg/L, glycine 3mg/L, thiamine hydrochloride 0.2mg/L, puridoxine hydrochloride 0.4mg/L, niacin 0.2mg/L, benzyl gland
Purine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, heteroauxin 2.2mg/L, plant cytokinin 3.0mg/L, agar
2%, surplus is distilled water.
4. a kind of production method of rice paddy seed according to claim 1, which is characterized in that described to take root in S400
Culture medium constituent be manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L,
Cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate 27.8mg/L, inositol 100mg/L, glycine
2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, benzyladenine 0.4mg/l, 6- chaff
Base adenine phosphate 0.4mg/l, heteroauxin 2.2mg/L, plant cytokinin 1.8mg/L, agar 2%, surplus is distilled water.
5. a kind of production method of rice paddy seed according to claim 1, which is characterized in that in S500, the richness
Selemium nutrition inorganic agent is made of the raw material of following weight parts: pine needle meal 6-9, myristic acid 1.3-2, sophorolipid 0.4-1, ferment
Female selenium 0.1-0.2, lauric acid amide of ethanol 0.1-0.3, cortex albiziae 2-3, pearl white 5-7, ethylmaltol 0.3-0.5, sulphur
The hydrogen peroxide 10-14 of sour ferrous iron 0.1-0.2, rose dry petal 4-6,5-7%.
6. a kind of production method of rice paddy seed according to claim 5, which is characterized in that the selenium-rich nutritive inorganic agent
Preparation method the following steps are included:
(1) above-mentioned pine needle meal is added in the sodium hydroxide solution of 10-13mol/l, the heat-insulation soaking 1-2h at 57-60 DEG C,
Precipitating washing 2-3 times is mixed with above-mentioned myristic acid, is added in the deionized water of 20-30 times of mixture weight by filtering,
Increasing temperature is 50-60 DEG C, and insulated and stirred 15-20 minutes, the hydrogen peroxide of above-mentioned 5-7% is added dropwise, and is stirred after being added dropwise to normal
Temperature must aoxidize pine needle meal solution；
(2) above-mentioned ethylmaltol, ferrous sulfate are mixed, is added in the deionized water of 57-60 times of mixture weight,
Insulated and stirred 10-17 minutes at 57-60 DEG C, maltol ferrous solution is obtained；
(3) above-mentioned sophorolipid is added in 20-30 times of its weight, 70-76% ethanol solution, raising temperature is 60-64
DEG C, insulated and stirred 20-30 minutes, above-mentioned yeast selenium is added, stirring to room temperature is mixed with above-mentioned maltol ferrous solution, and stirring is equal
It is even, above-mentioned lauric acid amide of ethanol is added, it is 3-5 minutes ultrasonic, obtain amide dispersion liquid；
(4) above-mentioned oxidation pine needle meal solution, amide dispersion liquid are mixed, are stirred evenly, remaining each raw material is added, stirs evenly,
Spray drying is to get the selenium-rich nutritive inorganic agent.
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| CN110777108A (en) * | 2019-11-22 | 2020-02-11 | 顾霆 | Mixed culture process flow of two kinds of cells |
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