CN110164279A - A kind of bladder urine flow turbulence shear stress simulation system - Google Patents
A kind of bladder urine flow turbulence shear stress simulation system Download PDFInfo
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Abstract
The invention discloses a kind of bladder urine flow turbulence shear stress simulation systems, which is characterized in that stirs instrument, urine reservoir, waste liquid storage bag, the first peristaltic pump, the second peristaltic pump including apery body bladder model container, motor；The apery body bladder model container is spherical glass bottle；The upper end of the spherical glass bottle is provided with stirring rod installation port, and the lower end of the spherical glass bottle is provided with urine inlet and urine outlet；The stirring rod of the motor stirring instrument connection is from stirring rod installation port insertion spherical glass bottle；The urine inlet is connected to the first peristaltic pump by two medical silicone tubes, and the first peristaltic pump is connected with urine reservoir；Urine outlet is connected to the second peristaltic pump by a medical silicone tube, and the second peristaltic pump is connected with waste liquid storage bag.System various dimensions simulation building turbulence shear stress of the present invention is huge for bacterial biof iotalm infection morbidity research application potential in body of urinary bladder.
Description
Technical field
The present invention relates to a kind of apparatus systems of simulation bladder urine storage urination, in particular to a kind of bladder urine flow turbulent flow, which is cut, answers
Power simulation system belongs to biomedical engineering technology field.
Background technique
Disease in the urological system disease incidence in mid-aged population is high, seriously affects the quality of life of mid-aged population,
It poses a huge burden on society.Disease in the urological system again based on lower urinary tract disease, secreting by organ of the bladder as storage of urine
In urinary system Disease, it is particularly acute once lesion influence occurs for bladder.
Existing medical technology would generally take certain active measures to mitigate the symptom of disease in the urological system, suffer to improve
Person's quality of life promotes the fast quick-recovery of disease.But reduced at present about the urine flowing situation of change research inside bladder, it controls
The often passive treatment for the treatment of process, so that therapeutic effect is poor, increases the puzzlement of patient.To find out its cause, existing biomaterial
Influencing each other with bladder, it is less to study, and causes situation in material application process unexpected, cannot be actively using access material.I.e.
Active material has been used, has also tended to that various new problems can be faced.
Because biomaterial and its curable product are in use, encountering to some extent cannot be complete with body tissue
A series of problems, such as compatible, calcification, infection, canceration.Wherein, and with biomaterial for medical purpose infections relating with biomaterial application
Increase, disease incidence dramatically increases, prevention and treatment biomaterial associated infection be clinical urgent problem to be solved.
Numerous studies are it has proven convenient that biomaterial for medical purpose In vivo infection key link is the shape of material surface bacterial biof iotalm
At.Although bacterial biof iotalm Forming Mechanism research work makes progress, however, we are in primary rank to the understanding of biomembrane
Section, Forming Mechanism do not illustrate yet.Know that bacterial biof iotalm forms including microorganism adhering, microcolony formation, maturation and divides
The solution stage, wherein bacterial adhesion conduit is the first step of urinary catheter associated urinary tract infections.It infects lasting key and is that bacterium is raw
Object film is formed, therefore, the key that prevention and treatment infection key is that blocking biofilm formation, and bacterial biof iotalm is prevented to be formed, then and
Source is formed from it takes steps to prevention bacterial adhesion.Undoubtedly, probing into bacterial adhesion mechanism becomes prevention and treatment biomaterial phase
Close the key link of infection.
It is the key link for researching and developing biomembrane infection new technology by appropriate experimental model, existing model is only studied
The correlation of external simple mechanical stimulation and part bacterial biof iotalm.Such as Chinese patent: CN 2265773Y, CN
101664313B、CN 102646351A、CN 102958467B、CN 103816576A、CN 104188734B、CN
The bladder simulation device system of the reports such as 1422684A is mostly single structure simulation, and fluid flow inside situation is single, lacks
Variation, result of study can not accurately react urine flowing inside practical bladder in organism.
Currently, there is not yet the Mechanobiology model of building simulation human bladder urine flow turbulence shear stress carries out internal material sense
The relevant report of model research is contaminated, the practical turbulent situation of change research of symmetrical bladder urine is insufficient, and biomembrane material is caused to be difficult to
Meet practical vivo environment requirement.Therefore, it needs a kind of binding and needs bladder urine turbulent flow changeable device, to improve biology
The progress of membrane material improves biomembrane compatibility, improves efficiency/quality of disease in the urological system treatment.
Summary of the invention
It is an object of the invention to overcome the bladder simulation in the presence of the prior art that can not effectively simulate urine inside bladder
Biofilm formation situation under liquid turbulent flow variable condition leads to bacterium easy to attach on biomaterial for medical purpose, and then bacterial membrane occurs
The deficiency of biological infection provides a kind of bladder urine flow turbulence shear stress simulation system.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical schemes:
A kind of bladder urine flow turbulence shear stress simulation system, including apery body bladder model container, motor stirring instrument, urine
Liquid reservoir, waste liquid storage bag, the first peristaltic pump, the second peristaltic pump.
The apery body bladder model container is spherical glass bottle.
The upper end of the spherical glass bottle is provided with stirring rod installation port, and the lower end of the spherical glass bottle is provided with urine
Liquid entrance and urine outlet.
The stirring rod of the motor stirring instrument connection is from stirring rod installation port insertion spherical glass bottle.
The urine inlet is connected to the first peristaltic pump by two medical silicone tubes, and the first peristaltic pump and urine reservoir connect
It connects.
The urine outlet is connected to the second peristaltic pump by a medical silicone tube, and the second peristaltic pump and waste liquid storage bag connect
It connects.
There is motor to stir instrument stirring rod inside the vial of apery body bladder model of the present invention, assembles all component constructions
" the preceding silicone tube-of the preceding peristaltic pump-of artificial urine-imitates human bladder's (load blender-film-forming carrier)-rear silicone tube-and wriggles afterwards
Pump-urine waste liquid " reaction system, turbulence shear stress apply: peristaltic pump tune urine speed, urine enter and leave reactor, electric blender rotation
Turn stirring urine flow, three dimension combined simulations construct turbulence shear stress.Simulation system is recycled using dual circuit feed liquor, may be implemented
The independently adjusting of feed liquor flow, flow velocity, in addition the rotation shearing force of blender stirring rod, parameters regulation can lead to
Computer control is crossed, realizes accurate real-time control load.Wherein, the medical silicone tube, also referred to as medical grade silicon rubber pipe, or
Person can also be replaced using medical rubber hose.
For mechanics angle, no matter fluid is in movement or stationary state, can generate the effect of power, and from power
Study biology angle, organism are among mechanical environment, and it is each that mechanics factor influences body, organ, tissue, cell and molecule
The biological process of level is rebuild including positive functional adaptation and is occurred with the destruction of the structure of negative sense and disease.
Bladder urine flow turbulence shear stress simulation system of the present invention realizes continuously adjustable variation design, more accurately and reliably
Simulation bladder urine flow stress variation situation, can be more accurate simulation body of urinary bladder in bacterial biof iotalm generate condition ginseng
Number, is of great significance for the Therapy study of disease in the urological system.Bladder urine flow turbulence shear stress simulation system of the present invention
According to stress-growth theory of " father of modern biotechnology mechanics " Y.C.Fung, planktonic bacteria (cell) is redeveloped into carefully
There are correlations with stress state inside and outside bacterium and biomembrane for the process of bacterium biomembrane (tissue).It is inquired into from Mechanobiology angle
Biomembrane infection, preferably can accurately research and analyse the case where bacterial growth adheres in bladder, be disease in the urological system patient
Bacterium prevention and treatment provides scientific guidance inside bladder in therapeutic process.
Further, apery body bladder model container is arranged in constant temperature incubator.Preferably, constant temperature incubator is controlled at 37
DEG C constant temperature.
Further, the volume 200-800mL of the spherical glass bottle.The volume of the spherical glass bottle and human bladder half
Full or expanded state volume is suitable, so that the total amount of liquid in experimentation in artificial urinary bladder is close to human body actual conditions,
So that result of study is more accurate and reliable, preferably from about 400ml.
Meanwhile the liquid for flowing through artificial urinary bladder can be adjusted according to physiological bladder capacity, volume and urinary excretion parameter
State (flow, flow velocity, turbulence intensity etc.) simulates the environmental quality that apposition growth occurs for bacterium inside human bladder, improves
Accuracy rate is studied, provides guidance for disease in the urological system treatment.
Turbulence shear stress: according to fluid mechanics principle, trigone cone-shaped when in conjunction with bladder inanition, it is full when approximation spheroid
Deformation process, passive diastole-actively shrinks the inertia of physiological property and bladder urine random motion, clearly proposes wing for the first time
Guang urine moves to form a kind of unique nonstationary flow ambient stress, i.e. turbulence shear stress (disturbed flow shear
Stress) load.
Technology standardization: 1. mycoderm reactor: customization about 800ml spherical glass tank simulates bladder, the outlet of bottom three simulation
Ureteral orifice and orificium urethrae internum；2. urine flow transfers access: medical silicone tube；3. turbulence shear stress applies: peristaltic pump tune urine speed,
Urine enters and leaves reactor, electric blender Stirring urine flow, and three dimension combined simulations construct turbulence shear stress.
Preferably, there are three connectors for the setting of spherical glass bottom of bottle portion.Three connectors respectively correspond urine inlet,
Urine outlet, wherein two connectors of urine inlet, one connector of urine outlet.
Further, first flowmeter is additionally provided between first peristaltic pump and urine reservoir.In artificial urinary bladder feed liquor
First flowmeter is set on pipeline and detects the total amount of liquid for entering artificial urinary bladder, facilitates and is filled inside Adjustment Tests research process bladder
It is full of the total amount of liquid.
Further, second flowmeter is additionally provided between second peristaltic pump and waste liquid storage bag.Artificial wing is detected simultaneously
Guang outlet conduit upper fluid total amount, preferably measure artificial urinary bladder inside total amount of liquid, accurately realize fluid flow, flow velocity,
The adjustment of the ambient conditions such as turbulent flow controls.
Alternatively, above-mentioned first flowmeter, second flowmeter can also be separately positioned on to the first peristaltic pump, the second wriggling
Between pump and apery body bladder model container.
Further, the first pulse damper is additionally provided between the first peristaltic pump and first flowmeter.
Further, the second pulse damper is additionally provided between the second peristaltic pump and second flowmeter.
Pulse damper is used to adjust the flow velocity fluctuation of peristaltic pump, and the flow rate of liquid for flowing into/flowing out model is more flat
Surely.
Further, the first pulse damper is additionally provided between first peristaltic pump and apery body bladder model container.
Further, the second pulse damper is additionally provided between second peristaltic pump and apery body bladder model container.
Pulse damper can also be directly set between peristaltic pump and apery body bladder model container, peristaltic pump is controlled
The pulse transport status of liquor charging body controls the whole steady conveying of liquid, keeps liquid inflow/rate of outflow in model permanent
Fixed, closer to the inflow and outflow feature of normal human's urine, the result for studying bacterial growth is more scientific reliable.
Further, the medical silicone tube isMedical silicone tube.Preferably, using the medical silicon of 14-16Fr
Sebific duct.Such as No. 10, No. 14, No. 16 silicone rubber tubes.First medical silicone tube and the second medical silicone tube are all made of 14-16Fr doctor
Use silicone tube.
Further, first peristaltic pump is distribution type intelligence peristaltic pump.Liquid quantitative suitable for various complexity distributes,
Suitable for accurate measurement and dosing, high-precision flow transmission control may be implemented, and can need to adjust according to experiment
Whole setting parameter automatization distribution.
Further, the body of rod surface of the stirring rod is loaded with biomedical research sample, and body of rod surface can load biology
Medical research sample.The biomedical research sample for stirring the load of club shaft surface stirs shearing force shape in stirring rod turbulent flow
Under state, can more efficient control shearing force intensity, realize different research effects.
The purpose of the present invention is the influences that urine turbulent condition forms bacterial membrane inside research bladder, using stirring rod
Surface as load biomedical research sample carrier, load effect it is more preferable, result of study is more accurate.
Compared with prior art, beneficial effects of the present invention:
1. bladder urine flow turbulence shear stress simulation system of the present invention, by establishing standardized apery body bladder urine flow turbulent flow
Shearing stress loading system is then applied to scientific research and clinic, and Mechanobiology morbidity will be infected for medical material bacterial biof iotalm
Mechanism Study and antibiont film infection medicine, new technology screening and the reliable experimental model of verifying offer, application prospect, doctor
It is self-evident with the importance of social benefit effect to learn value.
2. bladder urine flow turbulence shear stress simulation system of the present invention can adjust multiple parameters, institute under bladder environment is simulated
There is turbulent situation of change.It especially can be using stirring rod as the carrier for loading biomedical sample, so that carrier and liquid
Relative flow shear force it is adjustable controllable, high-precision analog study is realized with lower cost.For example, more famous
Commercial bacterium living beings membrane modle include Calgary biological membrane equipment and Disease Control and Prevention Center, U.S. biofilm reactor, the former is one
Kind upper cover has the microwell plate of pillar, and official's price is 500~$ of $ 3500, and the latter has 8 polypropylene rooms to constitute, contains respectively
3 samples (biofilm development surface) of culture solution package are suspended from for bacterial adhesion, main member official price is 860~$ of $
2200。
3. bladder urine flow turbulence shear stress simulation system of the present invention is stimulated with hydrodynamics to Escherichia coli Biofilm shape
At mechanics effect be point of penetration, using bladder stress path and biology of bacteria process as target spot is studied, voluntarily successfully to set
Based on the apery body bladder model bacterium living beings membrane reactor of meter, bladder urine turbulence shear stress environment is constructed, in analogue body
Bladder urine transfers physiology, bladder outlet obstruction pathologic process Cyclic Stress overall process, and urine flow stress stimulation skill is established in optimization
Art scheme constructs apery body bladder urine flow turbulence shear stress loading system.
It falls ill 4. bladder urine flow turbulence shear stress simulation system of the present invention can successfully simulate bacterial biof iotalm in body of urinary bladder
Mechanism, research application potential is huge, is of great significance for the overall quality promotion of apparatus system.
Detailed description of the invention:
Fig. 1 is the structural schematic diagram scheme one of apery body bladder model of the present invention.
Fig. 2 is the structural schematic diagram scheme two of apery body bladder model of the present invention.
Fig. 3 is biomembrane bacteria suspension smear.
Fig. 4 is that the counting of biomembrane bacteria suspension smear bacterium colony is compared.
Fig. 5 is that biomembrane bacteria suspension OD value compares.
Fig. 6 be CLSM bacterial biof iotalm imaging (from left to right be respectively for 24 hours, 72h, 120h and 168h, from top to bottom successively
For SAU, DAU1, DAU2, DAU3；Graduated scale=50 μm).
Fig. 7 is that mycoderm biomass compares.
Fig. 8 be mycoderm surface area ratio compared with.
Fig. 9 is that the mycoderm disperse distance that is averaged compares.
Figure 10 be SEM bacterial biof iotalm imaging (from left to right be respectively for 24 hours, 72h, 120h and 168h, from top to bottom successively
For SAU, DAU1, DAU2, DAU3；20Kv voltage, × 5000, graduated scale=20 μm).
Figure 11 is SEM bacterial biof iotalm surface area.
Marked in the figure: 1- liquid storing bag, 2- ellipsoid bodily form vial, 3- waste liquid storage bag, 4- blender, the first peristaltic pump of 5a-,
The second peristaltic pump of 5b-, the first pulse damper of 6a-, the second pulse damper of 6b-, 7a- first flowmeter, 7b- second flow
Meter, 9- constant temperature incubator, 10a- inlet tube, 10b- outlet tube.
Specific embodiment
Below with reference to test example and specific embodiment, the present invention is described in further detail.But this should not be understood
It is all that this is belonged to based on the technology that the content of present invention is realized for the scope of the above subject matter of the present invention is limited to the following embodiments
The range of invention.
<embodiment 1>
Artificial urinary bladder urine flow turbulence shear stress simulation system as shown in Figure 1, including spherical vial 2.The spherical
Vial 2 is radius 7cm, and the container of volume 800mL, 2 upper end of vial is provided with the opening of internal diameter 6cm, lower end base center
And three mouthfuls are opened by two sides, adjacent apertures spacing 3cm, opening inside diameter 0.8cm simulate Ureteral orifice and orificium urethrae internum.The glass
The upper end of glass bottle 2 is provided with the opening of upper-end inner diameter 6cm for installing stirring rod, and the lower end of the vial 2 is provided with urine
Entrance and urine outlet.After the vial upper end installation stirring rod, stirring rod and motor stirring instrument 4 are connected.Vial 2
The urine inlet of lower end is connected to the first pulse damper 6a by two medical grade silicon rubber pipes (inlet tube 10a), then connects
To the first peristaltic pump 5a.The input duct of first peristaltic pump 5a is connected to liquid storing bag 1 (material medical grade rubber, volume 5L).Urine
Outlet is connected to the second pulse damper 6b by a medical grade silicon rubber pipe (outlet tube 10b), is then attached to the second wriggling
Pump 5b.The liquid outlet of second peristaltic pump 5b is then connected to waste liquid storage bag 3 (material medical grade rubber, volume 3L).
Based on fluid mechanics principle, due to spherical glass bottle cavity specific form, stirring rod cloth is setting in spherical glass bottle
Middle part, when urine is less, stirring rod may not directly be contacted with urine or contact surface is smaller.So that studying
The agitation characteristics of a variety of different fluid states may be implemented in hydrodynamics in journey, especially urinates when in the vial of simulation bladder
During liquid measure increase/reduction, the case where referring to fluid flowing time to time change is had.So in corresponding model, it can
With urine delay, the variation etc. for simulating bladder urine consecutive variations, unique nonstationary flow ambient stress is formed, i.e., turbulent flow, which is cut, answers
Power.
Distribution of the unsteady turbulent motion for speed, pressure, temperature and material concentration in flow field, plays decisive
Influence." growth is decided by stress and strain ", organ and histocyte all rely on certain mechanical environment or by one
Determine the adjusting of mechanical stimulation.It is similar therewith, it is placed in biomaterial bacterial biof iotalm in vivo and is also at fluid stress environment, fluid force
It is equally an extremely important influence factor of urinary catheter surface bacteria biofilm formation.The unique turbulence shear stress of bladder is adjustable
Control escherichia coli in catheter surface Colonization and forms bacterial biof iotalm, and different stress parameters have respective mechanics biology
Learn effect.
Standardized model technical parameter is established by debugging to optimize repeatedly.Physiology is generated and drained according to normal urine, if
Fixed wherein major parameter: peristaltic pump rate of flooding range 0.005-1140mL/min, precision≤± 5, flow velocity 0.5-1.5mL/
Min, flow 62.5-83.33mL/h, the period be 1 urination/3-6h or continue urine turn of tidal stream, urinate flow rate>25mL/s or<
15mL/s.Artificial urine is persistently pumped into formation urine flow pond in bladder model, is persistently pumped into urine and flows through the bladder wall, urinary catheter and its ball
Irregular areas such as capsule, while compound interruption pumps out, and generates a kind of unsteady urine flow movement are cut with to simulate bladder urine flow turbulent flow and are answered
Power.
Physiology and pathological state urine flow stress loading scheme are established through the above scheme, it can be with further investigated bacterial biof iotalm
And infections relating forms pathologic process, and can be used for screening antibacterium biomembrane infection active drug and the reasonable antibiosis of exploitation
Object film treatment of infection new technology.
Production firm, model, the characteristic of equipment component used in experiment etc. are as follows:
First peristaltic pump and the second peristaltic pump are smart allocation type, single channel peristaltic pump, and design parameter is as follows:
Peristaltic pump, production firm: Baoding LanGe constant flow pump Co., Ltd, model: BT100-1F.
Pump head model: YZ1515X.
The specifically used characteristic of peristaltic pump is as follows: wriggling revolution speed range: 0.001-600.0rpm, positive and negative rotation are reversible.Revolving speed
Adjust resolution ratio: 0.1rpm.Control mode: external control can be carried out to peristaltic pump by standard external controlled interface and communication interface
System.Display mode: liquid crystal display shows flow and revolving speed simultaneously.Flux modification: will set the actual flow value that flow obtains and input,
It is automatic to carry out flux modification, flow accuracy control errors < 0.01%.Power-failure memory: can be according to the shape before power down after re-powering
State continues work.Full speed function: a key control full speed operation, for filling, emptying.Outer dimension (long × wide ×
It is high): < 200mm × 200mm × 200mm.Be applicable in power supply: AC 110V ± 10%/50W or AC 220V ± 10%/50W is optional.Work
Make environment temperature: 0 DEG C -40 DEG C.Working environment relative humidity: < 80%.Working time: stream time > 30 day are supported.
Hose: hose stream time > 30 day are equipped with.
First pulse damper, the second pulse damper are volume 50-100mL, the pulse damper of pressure-bearing highest 1MPa.
First flowmeter, second flowmeter key property are as follows:
Inlet and outlet: internal screw thread 5-6mm；Measuring accuracy: 1%；Repeatability 1%；Maximum working pressure (MWP): 1MPa；Operating voltage:
3V；Stand-by time: 2 years；Range of flow: 0.5ml-10ml/min.
Inlet tube, outlet tube use 16Fr (internal diameter about 5-6mm) single-chamber medical silicone tube.
Electric blender, production firm: Shanghai height causes precision instrument Co., Ltd together, model: GZJB-605.
Electric blender cooperation GTCS-2018 type speed control is controlled.Electric blender stirs impeller structure 4,
Diameter 13cm, bar long 35cm, revolving speed 0-60rpm.
Thermostatic chamber, Beijing Hai Fuda Science and Technology Ltd., model: DH-360AS.
<biological experiment research>
1, prepare simulation device system
Using artificial urinary bladder urine flow turbulence shear stress simulation system described in embodiment 1, biological test research is carried out.
2, the loading stress scheme model of simulation system is set
Setting static state and complicated dynamic two classes, four kinds of stress, including hydrostatic pressing (SAU), constant current shearing stress (DAU1), physiology
Shearing stress (DAU2), pathology shearing stress (DAU3) (table 1).
1 stress scheme of table
| Grouping | Experimental program |
| SAU | Micro-Organism Culture Dish loads 125ml artificial urine, replaces artificial urine for 24 hours |
| DAU1 | Peristaltic pump at the uniform velocity 1ml/min is entered and left, 15 revolutions per minute of blender (rpm) is at the uniform velocity |
| DAU2 | 5 times/urinate for 24 hours, every 4 hours primary, and 10 points to 6 points of morning of night are not urinated totally for 8 hours |
| DAU3 | 8 times/urinate for 24 hours, every 3 hours primary |
3, the characterization of reactor bacterial biof iotalm infection model is constructed
(1) mycoderm suspension smear counts bacterium colony (table 2, Fig. 3-4)
There were significant differences between different time (F value=6.30, P=0.0029), indifference (F between different urine flow stress groups
It is worth value=0.4001=1.11, P), reciprocation (F value=0.85, P value=0.5814) is not present in time and stress.Pass through figure
3, Fig. 4 line chart with stress time is applied as it can be seen that extend, and it is in increase trend that complex stress group bacterium colony, which counts, and especially pathology is answered
Power DAU3 group is significant.
2 biomembrane bacteria suspension smear bacterium colony of table counts
| Stress | 24h | 72h | 120h | 168h |
| SAU | 33±0 | 40±0 | 63±0 | 102.33±0.577 |
| DAU1 | 48±0 | 146.33±1.58 | 633±3.61 | 1143.67±5.69 |
| DAU2 | 110.67±3.05 | 555.67±4.72 | 2770.67±9.02 | 3610.67±10.07 |
| DAU3 | 2156.67±7.64 | 2593.33±8.33 | 3043±6.24 | 7995±6.24 |
As a result as shown in Figure 3-4, wherein Fig. 3 is biomembrane bacteria suspension smear.Fig. 4 is biomembrane bacteria suspension smear bacterium colony meter
Number compares
(2) mycoderm suspension OD value (OD) is measured
Between different urine flow stress groups, (F value distinguishes=110.84,187.96, P values between different time there are significant difference
< 0.0001), there are reciprocation (F value=50.05, P value < 0.0001) (tables 3) between time and stress.Above-mentioned OD value difference
Different and growth trend is as shown in figure 5, biomembrane bacteria suspension OD value comparison result.
3 biomembrane bacteria suspension OD value of table
| Stress | 24h | 72h | 120h | 168h |
| SAU | 0.002±0.001 | 0.004±0.001 | 0.0017±0.0011 | 0.0057±0.0006 |
| DAU1 | 0.0053±0.0011 | 0.002±0.0017 | 0.012±0.001 | 0.01766667±0.0006 |
| DAU2 | 0.0063±0.0006 | 0.007±0.0017 | 0.0033±0.0021 | 0.0083±0.0015 |
| DAU3 | 0.01±0 | 0.0083±0.0006 | 0.0077±0.0011 | 0.0273±0.0015 |
(3) mycoderm laser confocal microscope (CLSM) characterizes
With 2.1 quantitative analysis bacterium living beings film image of Image J 1.52K+Comstat, take biomass (Biomass,
BA, the volume of all voxels comprising mycoderm biomass is divided by area), surface area (Surface Area, SA, bacterial biof iotalm
Surface area) and average diffusion distance (Average Diffusion Distance, ADD, mycoderm bottom voxel to surface distance
Average length) 3 parameters are compared.
As a result be from left to right respectively as shown in fig. 6, CLSM bacterial biof iotalm is imaged, in figure for 24 hours, 72h, 120h and
168h is followed successively by SAU, DAU1, DAU2, DAU3 from top to bottom；Graduated scale=50 μm.
Between biomass difference urine flow stress group, between different time there are significant difference (F value difference=20.28,
201.90, P value=0.0004, < 0.0001), there are reciprocation (F value=29.85, P value < 0.0001) between time and stress
(table 4, Fig. 7).
Between surface area difference urine flow stress group, between different time there are significant difference (F value difference=3298.84,
3158.07, P values are < 0.0001), between time and stress there are reciprocation (F value=157.39, P value < 0.0001) (table 4,
Fig. 7-8).Fig. 7 is that mycoderm biomass compares, Fig. 8 be mycoderm surface area ratio compared with
Average disperse between different urine flow stress groups, between different time there are significant difference (F value difference=
192.74,371.67, P values are < 0.0001), there are reciprocation (F value=11.71, P value < 0.0001) between time and stress
(table 4, Fig. 9).Fig. 9 is that the mycoderm disperse distance that is averaged compares
4 bacterial biof iotalm biomass of table (μm3/μm2), surface area (μm2) and average diffusion distance (μm) compare
(4) mycoderm scanning electron microscope (SEM) characterizes
Bacterial biof iotalm is clapped through Gradient elution using ethanol, n-Amyl acetate displacement, ion sputtering instrument metal spraying plated film, upper machine testing
According to Adobe Photoshop CS3Extended+Image J 1.52K quantitative analysis bacterial biof iotalm.
Between surface area difference urine flow stress group, between different time there are significant difference (F value difference=505.72,
1201.84, P values are < 0.0001), between time and stress there are reciprocation (F value=78.14, P value < 0.0001) (table 5,
Figure 10-11).
As shown in Figure 10, the imaging of SEM bacterial biof iotalm (be from left to right respectively for 24 hours, 72h, 120h and 168h, from upper
Under be followed successively by SAU, DAU1, DAU2, DAU3；20Kv voltage, × 5000, graduated scale=20 μm).As shown in figure 11, SEM bacterium is raw
Object film surface product.
Table 5SEM mycoderm surface area (Surface Area, μm2)
| 24h | 72h | 120h | 168h | |
| SAU | 111715.67±12648.53 | 969131.33±41509.42 | 774200.00±36327.40 | 1524243.00±111963.60 |
| DAU1 | 165077.67±5626.77 | 1198918.00±8401.63 | 2065593.33±2674.42 | 2514373.67±75525.24 |
| DAU2 | 1002274.33±23040.50 | 1128964.67±12097.55 | 2841666.67±3737.23 | 3272319.33±371257.55 |
| DAU3 | 1096343.00±18778.92 | 1408327.00±160442.15 | 3556026.33±1863.86 | 4328239.00±21821.45 |
The above results show from bacterium colony counting, mycoderm suspension OD value and CLSM, SEM multi-angle Morphological Characterization, Escherichia coli
Biofilm formation is obvious in different stress group differences, and dynamic stress compares constant current and physiology compared to static stress, pathology stress
Stress difference is significant, meanwhile, as incubation time extends, mycoderm generates more obvious.
<embodiment 2>
Artificial urinary bladder urine flow turbulence shear stress simulation system as shown in Figure 2, including spherical vial 2, vial it is upper
End is provided with upper end opening and connects helical mixing blade for installing stirring rod, the bottom of stirring rod, and stirring rod top is connected to
Motor stirs instrument 4.The lower end of vial 2 is provided with urine inlet and urine outlet, the urine inlet of 2 lower end of vial is logical
It crosses two medical grade silicon rubber pipes and is connected to the first pulse damper 6a, be then attached to the first peristaltic pump 5a, hindered in the first pulse
First flowmeter 7a is additionally provided on pipeline between Buddhist nun's device and vial.Urine outlet passes through a medical grade silicon rubber pipe connection
To second flowmeter 7b, second flowmeter 7b connection the second pulse damper 6b, then the second pulse damper 6b is connected to
Two peristaltic pump 5b.The liquid outlet of second peristaltic pump connects waste liquid storage bag 3, and the liquid for testing discharge enters in waste liquid storage bag 3, separately makees
He uses.
For the scheme of embodiment 1, embodiment 2 is also provided with flowmeter, monitors the liquid in vial respectively
Total amount, the total amount of liquid of real time measure input and discharge vial.Alternatively, it is also possible to pass through the rate of pumping of peristaltic pump and time
Carry out the calculating of the total amount of liquid in vial 2.Carrying out detection feed flow using flowmeter obviously may be implemented preferably to flow
Metering precision is measured, and can be compared with the calculating structure of peristaltic pump, the reliability of experimental data is researched and analysed.
Claims (6)
1. a kind of bladder urine flow turbulence shear stress simulation system, which is characterized in that including apery body bladder model container, motor
Stir instrument, urine reservoir, waste liquid storage bag, the first peristaltic pump, the second peristaltic pump；
The apery body bladder model container is spherical glass bottle；
The upper end of the spherical glass bottle is provided with stirring rod installation port, and the lower end of the spherical glass bottle is provided with urine and enters
Mouth and urine outlet；
The stirring rod of the motor stirring instrument connection is from stirring rod installation port insertion spherical glass bottle；
The urine inlet is connected to the first peristaltic pump by two medical silicone tubes, and the first peristaltic pump is connected with urine reservoir；
Urine outlet is connected to the second peristaltic pump by a medical silicone tube, and the second peristaltic pump is connected with waste liquid storage bag.
2. bladder urine flow turbulence shear stress simulation system as described in claim 1, which is characterized in that apery body bladder model container
It is arranged in constant temperature incubator.
3. bladder urine flow turbulence shear stress simulation system as described in claim 1, which is characterized in that the appearance of the spherical glass bottle
Product 200-800mL.
4. bladder urine flow turbulence shear stress simulation system as described in claim 1, which is characterized in that the medical silicone tube is φ
The medical silicone tube of 6-18mm.
5. bladder urine flow turbulence shear stress simulation system as described in claim 1, which is characterized in that first peristaltic pump is point
Distribution type intelligence peristaltic pump.
6. bladder urine flow turbulence shear stress simulation system as described in claim 1, which is characterized in that the body of rod table of the stirring rod
Face is loaded with biomedical research sample.
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