CN110057729A - A kind of human body particulate matter passively settles concentration determination method - Google Patents
A kind of human body particulate matter passively settles concentration determination method Download PDFInfo
- Publication number
- CN110057729A CN110057729A CN201910185869.0A CN201910185869A CN110057729A CN 110057729 A CN110057729 A CN 110057729A CN 201910185869 A CN201910185869 A CN 201910185869A CN 110057729 A CN110057729 A CN 110057729A
- Authority
- CN
- China
- Prior art keywords
- particulate matter
- passively
- human body
- sampling
- settles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/0606—Investigating concentration of particle suspensions by collecting particles on a support
- G01N15/0612—Optical scan of the deposits
Abstract
The invention discloses a kind of human body particulate matters passively to settle concentration determination method, specifically this core technology of fluorescent grain trace labelling technology and particulate matter is combined passively to settle the passive exposed amounts of quantitative measurments human body particulate matter such as concentration sample method, particulate matter image sampling sample automatic counting method.Development will be analyzed Exposure before indirectly directly to test Exposure.The method of the present invention is integrated with particulate matter and passively settles the test method of concentration sample method, scaling method, test method and statistical method and can accurately test human body particulate matter and passively settle concentration.The human body particulate matter that is established as of the method for the present invention passively settles exposure analysis and provides possibility.It has very important significance for the research of particulate matter health threat.
Description
Technical field
The present invention relates to a kind of human body particle concentration test methods, and in particular to human body particulate matter passively settles concentration survey
Method for testing.
Background technique
The concept definition of personnel's exposed amount is for personnel to the exposure of substance in a certain environment.Personnel are to the sudden and violent of particulate matter
Dew amount can be further refined as the particulate matter exposed amount and passive Deposited particulate matter exposed amount that personnel actively suck.
Actively the particulate matter exposed amount of sucking is the particulate matter exposure for passing through breathing and entering human body, be can be used active
The instrument tests such as optical particle counter obtain.Particulate pollutant in air can also fall in human body by way of passively settling
The exposed surface such as skin, eyes.When these particulate matter Carried bacteria viruses or allergin, allergic dermatitis etc. can be caused
It is uncomfortable.The particulate matter for carrying the pollens such as artemisia, Chenopodiaceae, artemisiifolia is fallen in eyes, can cause blood-shoot-eye illness, anaphylaxis conjunctiva
The ophthalmology diseases such as inflammation.Statistics indicate that the whole world has 15%~20% crowd to suffer from anaphylactia, nearly 40% in these patients~
60% has ocular allergies symptom.In addition, particulate matter can also be deposited in garment surface, it is re-suspended into air by human motion,
And then by personnel's suction body.Meanwhile be deposited in the particulate matter on clothes can be transferred to human body elsewhere, to other people health
It threatens.Many researchs have been proven that effect of the clothes as particulate matter communication media before.
Before relative to there are mainly two types of the passive research methods for settling exposed amount.The first is optical particle counter test
Method.Make the particulate matter settling flux adhered on clothes by means such as beating, vibration, movements in Laboratory Module, utilizes optics instrument
Device tests the particle concentration and particle diameter distribution that settling flux generates.But this method can only study particulate matter settling flux on clothes
Situation can not study particulate matter adhesion condition on clothes.Simultaneously as the limitation of test method and instrument, is tested also not
Higher precision can be reached.Second is biological test method.Using the means of microbe research, research is adhered to thin on clothes
The concentration of the microbial aerosols such as bacterium, virus, pollen.But this method can only be confined to specific microbial aerosol.
It is therefore desirable to a kind of new human body particulate matter passively settles the accurate test method of concentration, in order to further investigate
Influence when grain object is passively exposed to the surfaces such as human eyeball, skin to human health.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of human body particulate matter passively to settle concentration determination side
Method solves the problems, such as that the research method precision of passively sedimentation exposed amount in the prior art is not high.
Technical solution of the present invention is summarized as follows:
A kind of human body particulate matter passively settles concentration determination method, the method be by the particulate matter settling amount method of sampling,
Particulate matter settling amount scaling method, fluorescent grain object trace labelling method, particulate matter image sampling sample automatic counting method group
At systematic analytic method.
The particulate matter settling amount method of sampling is divided into the glass sampling sheet method of sampling and the PTFE sampling film method of sampling,
Be respectively suitable for human body flat surface particulate matter passively settle concentration sample and non-smooth surface particulate matter passively settle it is dense
Degree sampling.
It is opposite when the particulate matter settling amount scaling method can be analyzed with quantitative analysis this method for different situations
Error condition.
The fluorescent grain object trace labelling method is realized by fluorescent grain object and high-resolution fluorescence microscope；Benefit
Human body package particulate matter is simulated with special fluorescent grain object, after being captured by the particulate matter settling amount method of sampling, in height
Fluorescence is issued under resolution ratio fluorescence microscope specific wavelength laser irradiation, and then shoots height using the CCD camera of fluorescence microscope
Resolution ratio particle photo.
The particulate matter image sampling sample automatic counting method is programmed using MATLAB software, and programming count is glimmering
Light microscope CCD camera shooting particle sampling sample image, specific method the following steps are included:
(1) gray scale pictures that CCD camera is shot are changed into pure black and white binary image by threshold transition method；Particulate matter is
The point of white, the place of particulate matter is not ater background；
(2) in the range of gray scale is 0-255, setting threshold value is 5, that is, thinks that place of the gray value greater than 5 is particulate matter；
(3) region being connected in bianry image is found out, the quantity for counting connected region is particulate matter quantity, so that it may
To under different situations, the passive exposure of human body different location.
Error between the automatic counting method and true value is within 5%.
The invention has the advantages that:
(1) it is integrated with the test that particulate matter passively settles concentration sample method, scaling method, test method and statistical method
Method can accurately test human body particulate matter and passively settle concentration.
(2) the human body particulate matter that is established as of this method passively settles exposure analysis and provides possibility.For particulate matter health
The research of threat has very important significance.
(3) the particulate matter settling amount method of sampling can test flat surface and the human body particulate matter of non-smooth surface passively sinks
Concentration drops.
(4) particulate matter image sampling sample automatic counting method can quickly and accurately calculate particulate matter on sampling apparatus
Number.Statistical time is reduced within 10% manually counted, and the error with true value is within 5%.
(5) foundation of this method will analyze Exposure development indirectly before directly to survey to Exposure
Examination.The exploitation that sensor is passively exposed for next step human body, which provides, researches and develops condition and technical support early period.
(6) foundation of this method is so that human body actively sucks pair of particulate matter exposed amount and passive settling particulate matter exposed amount
Than being possibly realized.Exploitation for novel human-body exposure model provides data and method.
Detailed description of the invention
Fig. 1 is each section method logic relation picture of the present invention and flow chart；
Fig. 2 is glass sampling sheet and PTFE sampling film method of sampling correlation calibration figure.
Specific embodiment
The invention will be further described in the following with reference to the drawings and specific embodiments.
The present invention characterizes passive settling particulate matter using specific fluorescent grain object.As shown in Figure 1, using particulate matter first
The particulate matter that the passive settling amount method of sampling carries out under specific time passively settles sampling.Secondly, being obtained using fluorescence microscope
Particulate matter image sample on high-resolution sample.Then, sample is counted using particulate matter image sampling sample automatic counting method
Particulate count on product.To obtain the passive exposure concentrations of particulate matter.Using particulate matter settling amount scaling method to different situations
Relative error when analysis carries out test analysis.Glass sampling sheet and PTFE sampling film method of sampling comparison mark is shown in Fig. 2
Fixed figure.
It is further detailed by taking certain dummy passively exposure experiment as an example.Indoor accelerated test ring is exposed in order to study
The passive exposed amount of human body in border is carried out using method described in the invention.Fluorescence is uniformly discharged to indoor using powder producer
Grain.It is added in BGI Collison generator after taking 1ml fluorescent particles stoste to dilute.According to calculating, every milliliter of fluorescent particles original
Liquid contains fluorescent grain 8.7 × 107It is a, it is assumed that be uniformly distributed on ground level in cabin, every photo can be seen under fluorescence microscope
To average 27 fluorescent grains.Substantially it can satisfy requirement.Agitation fan is opened, starts to send out dirt, hair dirt about needs 1h.In warm body
Passive sampling glass sampling sheet is placed with dummy.Sheet glass is removed after placing 2h.Carry out the fluorescence microscopy of next step.
This experiment uses Nikon 80i fluorescence microscope.The principle of fluorescence microscope is: not having when using common microscope
Laser excitation, fluorescent particles will not shine.It can not be easily by them and common granular zone under simple microscope
It separates.Red fluorescence particle can shine under the feux rouges excitation of specific wavelength, can be this specific glimmering using CCD camera
Granular photocatalytic material is captured and is taken pictures.Other fluorescent grain objects will not then shine.Under the light excitation of another specific wavelength, in addition
A kind of fluorescent grain object, such as green fluorescence particulate matter can just shine and be captured.It can be obtained by high-resolution sample in this way
Particulate matter image sample in sheet.The photo particles above object number of one sampling sheet can reach thousands of in many cases.So
Huge data volume can not method manually directly count.This is just needed by the particulate matter image sampling sample side of counting automatically
Method.Researcher develops an automatic calculating photo using MATLAB software (MathWorks Inc., Natick, MA, USA)
The program of upper particulate count.The gray scale pictures that CCD camera is shot are changed into the white binary map of black by threshold transition method first
Picture.Particulate matter is the point of white, and the place of particulate matter is not ater background.In the range of gray scale is 0-255, threshold is set
Value is 5, that is, thinks that place of the gray value greater than 5 is particulate matter.The region being connected in bianry image is found out, connected region is counted
Quantity is particulate matter quantity.It can be obtained by under different situations in this way, the passive exposure of human body different location.
Illustrative description has been done to the present invention above, it should explanation, the case where not departing from core of the invention
Under, any simple deformation, modification or other skilled in the art can not spend the equivalent replacement of creative work equal
Fall into protection scope of the present invention.
Claims (4)
1. a kind of human body particulate matter passively settles concentration determination method, which is characterized in that the method is by particulate matter settling amount
The method of sampling, particulate matter settling amount scaling method, fluorescent grain object trace labelling method, particulate matter image sampling sample are counted automatically
The systematic analytic method of counting method composition.
2. human body particulate matter passively settles concentration determination method according to claim 1, which is characterized in that the particulate matter
The settling amount method of sampling is divided into the glass sampling sheet method of sampling and the PTFE sampling film method of sampling, is respectively suitable for the smooth table of human body
The particulate matter in face passively settles concentration sample and the particulate matter of non-smooth surface passively settles concentration sample.
3. human body particulate matter passively settles concentration determination method according to claim 1, which is characterized in that the fluorescence
Grain object trace labelling method is realized by fluorescent grain object and high-resolution fluorescence microscope；Utilize special fluorescent grain object mould
Anthropomorphic body exposed grains object, it is specific in high-resolution fluorescence microscope after being captured by the particulate matter settling amount method of sampling
Wavelength laser irradiation is lower to issue fluorescence, and then shoots high-resolution particle photo using the CCD camera of fluorescence microscope.
4. human body particulate matter passively settles concentration determination method according to claim 1, which is characterized in that the particulate matter
Image sampling sample automatic counting method is programmed using MATLAB software, the shooting of programming count fluorescence microscope CCD camera
Particle sampling sample image, specific method the following steps are included:
(1) gray scale pictures that CCD camera is shot are changed into pure black and white binary image by threshold transition method；Particulate matter is white
Point, not the place of particulate matter be ater background；
(2) in the range of gray scale is 0-255, setting threshold value is 5, that is, thinks that place of the gray value greater than 5 is particulate matter；
(3) region being connected in bianry image is found out, the quantity for counting connected region is particulate matter quantity, so that it may be obtained not
With in situation, the passive exposure of human body different location.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910185869.0A CN110057729A (en) | 2019-03-12 | 2019-03-12 | A kind of human body particulate matter passively settles concentration determination method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910185869.0A CN110057729A (en) | 2019-03-12 | 2019-03-12 | A kind of human body particulate matter passively settles concentration determination method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110057729A true CN110057729A (en) | 2019-07-26 |
Family
ID=67316235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910185869.0A Pending CN110057729A (en) | 2019-03-12 | 2019-03-12 | A kind of human body particulate matter passively settles concentration determination method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110057729A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114018771A (en) * | 2021-10-29 | 2022-02-08 | 中车青岛四方机车车辆股份有限公司 | Method for detecting liquid particles in indoor flow field |
| CN114486664A (en) * | 2022-02-14 | 2022-05-13 | 北京大学 | System and method for capturing motion trail of particulate matter |
| CN115165687A (en) * | 2022-07-14 | 2022-10-11 | 西北核技术研究所 | Device and method for testing dry settling velocity of micron-sized solid particles |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101952709A (en) * | 2007-11-15 | 2011-01-19 | 爱克斯崔里斯科技有限公司 | Particle detection |
| CN102539295A (en) * | 2012-02-16 | 2012-07-04 | 南京信息工程大学 | Transgenic crop pollen real-time image automatic acquisition instrument and method |
| CN105547944A (en) * | 2016-01-11 | 2016-05-04 | 河北工程大学 | Inhalable particle detection method based on superimposition diffraction |
| CN105574493A (en) * | 2015-12-14 | 2016-05-11 | 常熟理工学院 | Source analysis method for diesel engine combustion particles |
| CN105891065A (en) * | 2016-04-07 | 2016-08-24 | 严媚 | Method for detecting particles in air on basis of contact-type image sensing |
| CN107560987A (en) * | 2017-09-11 | 2018-01-09 | 重庆交通大学 | A kind of air bioparticle concentration computational methods |
| CN108698043A (en) * | 2015-08-27 | 2018-10-23 | 阿提维医疗公司 | Fluid keeps and distributes micro- feature |
| CN108693098A (en) * | 2017-04-05 | 2018-10-23 | 奥林巴斯软成像解决方案公司 | The cell count analysis method of cell sample |
-
2019
- 2019-03-12 CN CN201910185869.0A patent/CN110057729A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101952709A (en) * | 2007-11-15 | 2011-01-19 | 爱克斯崔里斯科技有限公司 | Particle detection |
| CN102539295A (en) * | 2012-02-16 | 2012-07-04 | 南京信息工程大学 | Transgenic crop pollen real-time image automatic acquisition instrument and method |
| CN108698043A (en) * | 2015-08-27 | 2018-10-23 | 阿提维医疗公司 | Fluid keeps and distributes micro- feature |
| CN105574493A (en) * | 2015-12-14 | 2016-05-11 | 常熟理工学院 | Source analysis method for diesel engine combustion particles |
| CN105547944A (en) * | 2016-01-11 | 2016-05-04 | 河北工程大学 | Inhalable particle detection method based on superimposition diffraction |
| CN105891065A (en) * | 2016-04-07 | 2016-08-24 | 严媚 | Method for detecting particles in air on basis of contact-type image sensing |
| CN108693098A (en) * | 2017-04-05 | 2018-10-23 | 奥林巴斯软成像解决方案公司 | The cell count analysis method of cell sample |
| CN107560987A (en) * | 2017-09-11 | 2018-01-09 | 重庆交通大学 | A kind of air bioparticle concentration computational methods |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114018771A (en) * | 2021-10-29 | 2022-02-08 | 中车青岛四方机车车辆股份有限公司 | Method for detecting liquid particles in indoor flow field |
| CN114486664A (en) * | 2022-02-14 | 2022-05-13 | 北京大学 | System and method for capturing motion trail of particulate matter |
| CN114486664B (en) * | 2022-02-14 | 2023-12-22 | 北京大学 | Particle motion track capturing system and method |
| CN115165687A (en) * | 2022-07-14 | 2022-10-11 | 西北核技术研究所 | Device and method for testing dry settling velocity of micron-sized solid particles |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110057729A (en) | A kind of human body particulate matter passively settles concentration determination method | |
| JP6505714B2 (en) | Dosimeter including lensless imaging system | |
| JP2022058419A (en) | System and method for cell levitation and monitoring | |
| EP1651947B1 (en) | Device for counting micro particles | |
| US20160231224A1 (en) | Method and device for high throughput cell depormability measurements | |
| CN106520537B (en) | A kind of the micro-fluidic optical analysis system and analysis method of T cell immune response | |
| Zeng et al. | A low cost and portable smartphone microscopic device for cell counting | |
| Broßell et al. | A thermal precipitator for the deposition of airborne nanoparticles onto living cells—Rationale and development | |
| CN1826521A (en) | Device for counting micro particles | |
| US20190234853A1 (en) | Holographic characterization using hu moments | |
| Yang et al. | Portable rice disease spores capture and detection method using diffraction fingerprints on microfluidic chip | |
| JPS58196440A (en) | Method of arranging grain in fluidized fluid sample | |
| CN110057730A (en) | A kind of human body particulate matter active inhaled concentration test method | |
| US20240094193A1 (en) | High-throughput imaging platform | |
| Maw et al. | A changeable lab-on-a-chip detector for marine nonindigenous microorganisms in ship’s ballast water | |
| Kang | Quantification of blood viscoelasticity under microcapillary blood flow | |
| CN113063786A (en) | Urine visible component analyzer based on micro-nano fluidic chip and use method thereof | |
| CN103760073A (en) | Device and testing method for simulating indoor dust flying-off and settling property | |
| Dai et al. | Cell image reconstruction for a lens-free imaging system based on linear array sensor | |
| CN109055213A (en) | A kind of production method of simple Micro-CPE neutralization test and the device and the device of counting | |
| US20190078994A1 (en) | Multiplexed analysis of cell-materials in niches | |
| Jagannadh et al. | Field-portable microfluidics-based imaging flow cytometer | |
| CN116698679B (en) | Real-time particle filtering effect measuring device and system | |
| CN203688407U (en) | Device for simulating fly over and settlement characteristics of dust in room | |
| de Geus et al. | 76 Customizable polymer-based synthetic cells for imaging flow cytometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190726 |