CN1100271A - Microecological carp preparation and its preparing method - Google Patents
Microecological carp preparation and its preparing method Download PDFInfo
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- CN1100271A CN1100271A CN94110194A CN94110194A CN1100271A CN 1100271 A CN1100271 A CN 1100271A CN 94110194 A CN94110194 A CN 94110194A CN 94110194 A CN94110194 A CN 94110194A CN 1100271 A CN1100271 A CN 1100271A
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- carp
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The present invention relates to microecological carp prepn produced with lactobacillaceae and arthrobacter obtained from the enteron of normal carp and prepared into vital bacillus powder through industrial propagation. The bacillus powder is mixed into fish feed for use and it can adjust the bacillus group in fish enteron so as to promote the growth. Test shows that the said prepn can result in an increase of carp production of 25% and a decrease of feed coefficient of 0.75.
Description
The present invention relates to a kind of probiotics made from Lactobacillus casei and arthrobacterium live body bacterium powder and preparation method thereof.This probiotics can be widely used in propagating artificially of carp and other fresh water food fish as a kind of additive of bait.
Aspect fish diseases prevented and treated, traditional method was chemotherapy or uses antibiotics, but because chemical sproof generation, the accumulation of antibiotic in the fish body etc. have influenced the quality of aquatic products, even caused harm to human health.For this reason, both at home and abroad all in research biological control or bionomic control technology, in the hope of addressing the above problem.The external health element that has people raiser is promptly adjusted human enteric bacteria with that live or dead participation and is kept intestinal eubiosis, promote to grow, thereby indirect disease preventing and treating improves health.At present both at home and abroad people and animals prevent and cure diseases and health care aspect utilize lactobacillus, Bifidobacteriums etc. are developed a collection of new probiotics, obtain good result.Aspect aquaculture, the human Bacillus cereus is also arranged as the common eel open-mouthed bait, improved survival rate of fish fry.But the research report that adds ecological goods in bait is seldom, in the reality extensive use to remain with vitamin, amino acid, mineral matter etc. be the additive of bait of main component.
The present invention is according to the microecology principle, isolate Lactobacillus casei and arthrobacterium is made live body bacterium powder through passage and attenuation, fermentating breeding, concentrate drying from normal carp enteron aisle, and as the additive of carp and other fresh water food Technique in Fishes bait, ingest by fish and to have the bait of active bacteria formulation, the viable bacteria body is returned the fish body, adjust the gut flora of fish, the fish body is grown in the best condition, thereby realize preventing and curing diseases and promote the effect of fish bulk-growth, weightening finish.
Two kinds of bacterial strains provided by the invention all are the dominant bacterias in the normal carp enteron aisle.Identify through institute of microbiology of the Chinese Academy of Sciences, wherein a kind of is Lactobacillus casei rhamnose subspecies (Laecto-bacillus casei subsp rhamnosus), be Gram-positive, shaft-like, single, paired, chaining, atrichia, no pod membrane and brood cell, amphimicrobian, well-grown on the Lbs culture medium, 15～45 ℃ of growths, 37 ℃ of the bests, pH value below 4.0,8.0 above undergrowths, exotoxin in not having, numbering JY31; Another kind is a bacterial classification of arthrobacterium (Arthrobater sp), be Gram-positive, shaft-like, old cell is spherical, exotoxin, no brood cell, atrichia and pod membrane in the amphimicrobian, nothing, well-grown on the NB culture medium, 15～38 ℃ of growths, 37 ℃ of growths are best.PH value is 8.0 above undergrowths below 5.0, numbering JY10.This two strains bacterium has been sent China Committee for Culture Collection of Microorganisms's preservation, preserving number 0211(JY10 on March 26th, 1994) and 0212(JY31).
The microecological carp preparation that the present invention is alleged is to form with at least a live body bacterium powder of JY31 and JY10 bacterial strain.In the Mixed Microbes powder, the best ratio of the viable bacteria of JY31 and JY10 amount is 1:1.Its preparation method is JY31 and JY10 bacterial strain to be inoculated in respectively on the culture medium of Different Nutrition cultivate, and carries out passage and attenuation, and the bacterial classification with domestication is inoculated in fermented and cultured in the different zymotic fluids more respectively then, makes live body bacterium powder through concentrate drying.Live body bacterium powder can be measured quantity, makes it to reach certain index, then two kinds of bacterium powder is mixed according to the number of viable ratio of 1:1, packs.
The bacterial classification passage and attenuation of JY31 and JY10 bacterial strain can be distinguished the Lbs of elder generation in standard, inoculate on the NB agar slant culture-medium, and then the difference culture transferring is in the Lbs of standard, the NB broth bouillon, inoculum concentration is that 0.1～1%, 22～40 ℃ of conditions are carried out, and incubation time is 24 hours.
The preparation of JY31 bacterium powder can be finished as follows: (1) is inoculated in the JY31 seed liquor in the soup zymotic fluid of bean sprouts and ferments, condition is 22～40 ℃, pressure 0.1mpa, stir the 30-50rpm/ branch, cultivated, and made viable count reach 20～3,000,000,000/ml in 30～36 hours; (2) zymotic fluid is emitted, obtain bacterium mud through supercentrifuge (16000rpm) processing; (3) with the Carbon Dioxide calcium absorption bacterium muddy water branch that contains 5% milk powder, fully mix, in 60-80 ℃ of baking box, did roasting 30 minutes, mix, do roasting, repeated multiple times again and obtain dry bacterium powder.
The preparation of JY10 bacterium powder is to finish as follows: (1) is inoculated in fermented and cultured in the soup zymotic fluid of bean sprouts with the JY10 seed liquor, condition is 22～38 ℃, and pressure 0.1mpa stirs 60～100rpm/ branch, incubation time 24～36 hours makes the viable count of zymotic fluid reach 20～3,000,000,000/ml; (2) zymotic fluid is emitted, obtain bacterium mud through supercentrifuge (16000rpm) processing; (3) with the Carbon Dioxide calcium absorption bacterium muddy water branch that contains 5% milk powder, fully mix, in 60～80 ℃ of baking boxs, do roastingly, mix repeatedly, driedly bake, make dry bacterium powder.
Above-mentioned said JY31 zymotic fluid can be to calculate with dried soya bean at 5%() bean sprouts soup in add NaCl1%, yeast extract 0.3%, KaHPO
40.1%, NaH
2PO
40.01%, pH value is 6-7, best 6.5; And the JY10 zymotic fluid can be to calculate with dried soya bean at 5%() bean sprouts soup in add NaCl10.5%, glucose 1%, beef extract 0.3%, yeast extract 0.3%, pH value are 6.5～8, best 7.5.
The present invention is the 2 strain dominant bacterias that isolated 32 strain Gram-positive bacillus select in 54 samples of 9 batches 27 tail carps.Use JY31, JY10 bacterium powder adds the bait of making, and carp and other fresh water food fish rate of body weight gain, energy assimilability, ecological growth rate are improved, and feed coefficient, consumption rate descend, and positive role is arranged to improving fish body immune level and premunition, have the effect of preventing and curing diseases.
The embodiment of the invention is as follows:
Embodiment one: JY31 and the experiment of JY10 culture medium.The aerobic culture medium NB of selection standard, Emb sb and standard anaerobic culture medium Bs, Lbs compare culture experiment respectively in culture tank, the result is that JY10 is the best with the NB culture medium, and JY31 is the best with the Lbs culture medium.
Embodiment two: JY31 and the experiment of JY10 culture medium.Select Lbs and NB standard medium respectively, pH value sets 6,0,6,5 respectively, and 7,0,7,5,8,0 cultivate contrast test.The result is: JY31 has growth in PH6.0～7.0 scopes, and best pH value is 6.5; JY10 has growth in PH6.5～8.0 scopes, best pH value is 7.5.
Embodiment three: JY31 and the experiment of JY10 optimum culturing temperature.Select Lbs(JY31) and NB(JY10) standard medium, pH value is respectively 6.5 and 7.5 and sets 22 ℃, 37 ℃, respectively JY31 and JY10 is cultivated contrast test for 40 ℃.The result is: the optimum temperature of two strain bacterium is 37 ℃, but the JY31 bacterial strain still can have the growth of some when 40 ℃ of conditions.
Embodiment four: application experiment of the present invention.Bacterium powder viable bacteria amount JY31 is 1,000,000,000/g, and JY10 is 1,000,000,000/g, mixes according to 1:1 number of viable ratio, and every 10g Mixed Microbes powder is sprayed on the 50kg bait material with 2000ml water dilution mixing, fully mixes and dries in the shade and throw something and feed.Select three fish farms to throw something and feed, carry out fish body weight gains, growth resistance against diseases influence experiment.
First experimental point: green stream River Reservoir net cage is supported carp.Totally 4 group of 80 tail experiment fish contrasts 4 group of 80 tail.
Second tests a little: fish farm, mountain range, the Southern Pass, carp is supported in the pond.Totally 4 group of 80 tail experiment fish contrasts 4 group of 80 tail.
The 3rd tests a little: Dahuofang Reservoir, Fushun City net cage is supported carp, and totally 3 group of 60 tail experiment fish contrasts 3 group of 60 tail.
1. to fish body weight gains, growth promoting effect
First experimental point: rate of body weight gain: experimental group is 43%, and control group is 30.3%; Feed coefficient: experimental group is 2.07, and control group is 2.83; The energy assimilability: experimental group is 25%, control group 19.3%; The tissue growth rate: experimental group is 65%, and control group is 48.3%.
Second experimental point: rate of body weight gain: experimental group is 49%, and control group is 29%; Feed coefficient: experimental group is 1.89, and control group is 3.01; The energy assimilability: experimental group is 37.4%, and control group is 24.6%; Ecological growth efficiency: experimental group is 23%, and control group is 10.7%; The tissue growth rate: experimental group is 60.3%, and control group is 43.4%.
The 3rd experimental point: rate of body weight gain: experimental group is 149%, control group 134%; The forage fiss number: experimental group is 2.9, and control group is 3.64; The energy assimilability: experimental group 33.3%, control group are 25.4%; Ecological growth efficiency: experimental group is 19.3%, and control group is 13.5%; The tissue growth rate: experimental group is 58%, and control group is 53.2%.
2. the influence of enantiopathy power
1. neutrophil leucocyte phagocytic function (infecting) with gartner's bacillus
Do not infect: experimental group: phagocytic rate 69.83 ± 1.47 phagocytic index 2.96 ± 0.1
Control group: phagocytic rate 57.4 ± 1.14 phagocytic index 2.55 ± 0.07
Through infecting: experimental group: phagocytic rate 77.5 ± 1.29, phagocytic index 3.66 ± 0.36
Control group: phagocytic rate 70.3 ± 0.59, phagocytic index 3.25 ± 0.34
2. macrophage phagocytic function
Do not infect: experimental group: 63.01 ± 4.64
Control group: 53.44 ± 1.14
Infect the back: experimental group: 70.2 ± 4.1
Control group: 55.5 ± 1.14
3. E rosettes rate:
Do not infect Ea (%) Et (%)
Experimental group 25.5 ± 0.71 35.25 ± 1.5
Control group 23.67 ± 2.5 38.5 ± 0.71
Infect back Ea (%) Et (%)
Experimental group 25.17 ± 2.04 32.0 ± 1.83
Control group 10.5 ± 0.71 19.0 ± 1.47
3. to every physiology, the influence of biochemical indicator
1. RBC number, Hb content, the blood plasma total protein concentration, blood sugar amount, experimental group all are higher than control group; Blood fat amount and fragility of erythrocytes, experimental group is lower than control group.
2. muscle moisture content, experimental group is lower than control group: muscle contains protein rate and fat content, and experimental group is higher than control group.
Claims (9)
1, a kind of microecological carp preparation is characterized in that said preparation is that at least a live body bacterium powder of Cheesecake lactobacillus rhamnose subspecies (Lactobacillus Casei Subsp rhamnosus. numbers JY31) bacterial strain and arthrobacterium (Arthrobacter sp numbers JY10) bacterial strain is formed.
2, microecological carp preparation according to claim 1, the viable bacteria amount that it is characterized in that JY31 in the Mixed Microbes powder and JY10 is than being 1:1.
3, microecological carp preparation according to claim 1, its preparation method is characterised in that: JY31 bacterial strain and JY10 bacterial strain are inoculated in respectively cultivate bacterial classification on the culture medium, then the bacterial strain kind liquid of cultivating is inoculated in the ferment of setting out in the zymotic fluid respectively and cultivates, through the bacterium powder that concentrates, drying is made live body respectively.
4, the preparation method of microecological carp preparation according to claim 3, it is characterized in that will obtain live body bacterium powder measure quantity, then with JY31 and the JY10 bacterium powder after quantitatively by the mixing of 1:1 ratio, pack.
5, the preparation method of microecological carp preparation according to claim 3 is characterized in that JY31, and the kind liquid culture medium of JY10 bacterial strain is respectively the Lbg agar slant of standard, Lbg meat soup and NB agar slant, NB meat soup.
6, the preparation method of microecological carp preparation according to claim 3, the preparation that it is characterized in that JY31 bacterium powder is to finish as follows: (1) is inoculated in the JY31 seed liquor in the soup zymotic fluid of bean sprouts and ferments, condition is 22-44 ℃, pressure 0.1mPa, stir the 30-50rpm/ branch, cultivated, and made viable count reach 20-30 hundred million/ml in 30-36 hour; (2) zymotic fluid is emitted, obtain bacterium mud through the supercentrifuge centrifugal treating; (3) with the Carbon Dioxide calcium absorption bacterium muddy water branch that contains with 5% milk powder, the abundant mixing, in 60-80 ℃ of baking box, baked 30 minutes, mix again, to do and bake, repeated multiple times obtains dry bacterium powder.
7, the preparation method of microecological carp preparation according to claim 3, the preparation that it is characterized in that JY10 bacterium powder is to finish as follows: (1) is inoculated in fermented and cultured in the soup zymotic fluid of bean sprouts with the JY10 seed liquor, condition is 22-40 ℃, pressure 0.1mPa, stir the 60-100rpm/ branch, 24-36 hour, make the zymotic fluid viable count reach 20-30 hundred million/ml; (2) zymotic fluid is emitted, obtain bacterium mud through the supercentrifuge centrifugal treating; (3) with the Carbon Dioxide calcium absorption bacterium muddy water branch that contains 5% milk powder, fully mixing, dried roasting in 60-80 ℃ of baking box, mix repeatedly, do and bake, make dry bacterium powder.
8,, it is characterized in that said zymotic fluid is to add NaCl 1%, yeast extract 0.3%, KaHPO in 5% bean sprouts soup according to the preparation method of claim 3 or 6 described microecological carp preparations
40.1%, NaH
2PO
40.01%, pH value is 6-7.
9, according to the preparation method of claim 3 or 7 described microecological carp preparations, it is characterized in that said zymotic fluid is to add NaCl 0.5% in 5% bean sprouts soup, glucose 1%, beef extract 0.3%, yeast extract 0.3%, pH value are 6.5-8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94110194A CN1100271A (en) | 1994-04-12 | 1994-04-12 | Microecological carp preparation and its preparing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94110194A CN1100271A (en) | 1994-04-12 | 1994-04-12 | Microecological carp preparation and its preparing method |
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|---|---|
| CN1100271A true CN1100271A (en) | 1995-03-22 |
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|---|---|---|---|
| CN94110194A Pending CN1100271A (en) | 1994-04-12 | 1994-04-12 | Microecological carp preparation and its preparing method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292795C (en) * | 2002-07-15 | 2007-01-03 | 诺瓦提斯公司 | Vaccine against salmonid rickettsial septicaemia based on arthrobacter cells |
| CN101088362B (en) * | 2006-06-13 | 2012-09-05 | 上海创博生态工程有限公司 | Fish and shrimp phagostimulant of fermented product adhesion protein and its preparation process |
| CN115992062A (en) * | 2022-08-22 | 2023-04-21 | 中国农业科学院饲料研究所 | Lactobacillus rhamnosus calcium salt culture and preparation method and application thereof |
-
1994
- 1994-04-12 CN CN94110194A patent/CN1100271A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292795C (en) * | 2002-07-15 | 2007-01-03 | 诺瓦提斯公司 | Vaccine against salmonid rickettsial septicaemia based on arthrobacter cells |
| CN101088362B (en) * | 2006-06-13 | 2012-09-05 | 上海创博生态工程有限公司 | Fish and shrimp phagostimulant of fermented product adhesion protein and its preparation process |
| CN115992062A (en) * | 2022-08-22 | 2023-04-21 | 中国农业科学院饲料研究所 | Lactobacillus rhamnosus calcium salt culture and preparation method and application thereof |
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