CN110004141A - Nucleic acid extraction method and its extraction cassette - Google Patents
Nucleic acid extraction method and its extraction cassette Download PDFInfo
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- CN110004141A CN110004141A CN201811535018.6A CN201811535018A CN110004141A CN 110004141 A CN110004141 A CN 110004141A CN 201811535018 A CN201811535018 A CN 201811535018A CN 110004141 A CN110004141 A CN 110004141A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/04—Solvent extraction of solutions which are liquid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Abstract
The present invention discloses a kind of nucleic acid extraction method and its extraction cassette, and extraction cassette includes a liquid storage module and an extraction module.Above-mentioned extraction module is connected to above-mentioned liquid storage module.Above-mentioned extraction module includes an extraction module body, an expansion room, a reaction chamber, a filter membrane, a collecting chamber, one first waste liquid chamber and one second waste liquid chamber.Expand room and is formed in above-mentioned extraction module body.Reaction chamber is formed in above-mentioned extraction module body, and reaction chamber includes reaction chamber outlet, reaction chamber outlet and a reaction chamber connection breach.Filter membrane is set to above-mentioned reaction chamber and corresponding above-mentioned reaction chamber outlet.Collecting chamber is formed in above-mentioned extraction module body, and is connected to above-mentioned reaction chamber outlet.First waste liquid chamber is formed in above-mentioned extraction module body, and above-mentioned first waste liquid chamber is connected to above-mentioned reaction chamber outlet.Second waste liquid chamber is formed in above-mentioned extraction module body, and above-mentioned second waste liquid chamber is connected to above-mentioned reaction chamber outlet.
Description
Technical field
The embodiment of the present invention is related to a kind of extraction cassette, in particular to a kind of extraction cassette with liquid storage module.
Background technique
The nucleic acid extraction module of the prior art mainly only provides the function of nucleic acid extraction, and does not have for accommodating waste liquid
Waste liquid chamber design.The indoor filter membrane of reaction is polluted since waste liquid may flow back, in the prior art, waste liquid chamber is being set
Setting will not neighbouring collecting chamber.The size of cassette is extracted under disparate modules external member since waste liquid chamber and reaction chamber need to be set to
What is become is very huge, not only takes up space, also promotes cost.
The prior art there is also the nucleic acid extraction module of complex function (all-in-one), using magnetic bead extracting process come
Nucleic acid extraction is carried out, but the extraction efficiency of magnetic bead and purity are bad.
Summary of the invention
A kind of extraction cassette that the embodiment of the present invention provides in order to solve problems in the prior art, including a liquid storage mould
Block and an extraction module.Above-mentioned extraction module is connected to above-mentioned liquid storage module.Above-mentioned extraction module include one extraction module body,
One expands room, a reaction chamber, a filter membrane, a collecting chamber, one first waste liquid chamber and one second waste liquid chamber.Expand room to be formed in
State extraction module body.Reaction chamber is formed in above-mentioned extraction module body, including a reaction chamber inlet, reaction chamber outlet and
One reaction chamber connection breach, wherein above-mentioned expansion room connects above-mentioned reaction chamber connection breach, and above-mentioned reaction chamber connection breach are located at
Between above-mentioned reaction chamber inlet and the outlet of above-mentioned reaction chamber.Filter membrane is set to above-mentioned reaction chamber and corresponding above-mentioned reaction chamber outlet.It receives
Collection room is formed in above-mentioned extraction module body, and is connected to above-mentioned reaction chamber outlet.First waste liquid chamber is formed in above-mentioned extraction module
Ontology, above-mentioned first waste liquid chamber are connected to above-mentioned reaction chamber outlet.Second waste liquid chamber is formed in above-mentioned extraction module body, and above-mentioned
Two waste liquid chambers are connected to above-mentioned reaction chamber outlet.
In one embodiment, above-mentioned reaction chamber includes a taper portion, and above-mentioned filter membrane is set to above-mentioned taper portion, above-mentioned reaction chamber
Outlet is formed in the end of above-mentioned taper portion.
In one embodiment, above-mentioned extraction module further includes a first passage and a second channel, above-mentioned first passage
It is connected to above-mentioned reaction chamber outlet and above-mentioned first waste liquid chamber and above-mentioned second waste liquid chamber, above-mentioned second channel is connected to above-mentioned reaction
Room outlet and above-mentioned collecting chamber.
In one embodiment, above-mentioned first passage and above-mentioned second channel export in above-mentioned reaction chamber and intersect, and above-mentioned the
A barricade is formed in two channels, above-mentioned barricade is formed in the end of above-mentioned second channel and adjacent above-mentioned reaction chamber outlet.
In one embodiment, above-mentioned extraction module further includes a third channel and an above-mentioned fourth lane, above-mentioned third
Channel connects above-mentioned first passage and above-mentioned first waste liquid chamber, and above-mentioned fourth lane connects above-mentioned first passage and above-mentioned the
Two waste liquid chambers.
In one embodiment, above-mentioned first waste liquid chamber includes one first waste liquid chamber connecting hole, and above-mentioned second waste liquid chamber includes
One second waste liquid chamber connecting hole, above-mentioned third channel connects above-mentioned first passage and above-mentioned first waste liquid chamber connecting hole, above-mentioned
Fourth lane connects above-mentioned first passage and above-mentioned second waste liquid chamber connecting hole, above-mentioned second waste liquid chamber are at least partially disposed at
It states between the first waste liquid chamber connecting hole and above-mentioned second waste liquid chamber connecting hole.
In one embodiment, at least part of above-mentioned fourth lane extends along a first direction, and above-mentioned first direction is separate
Above-mentioned first waste liquid chamber.
In one embodiment, above-mentioned second waste liquid chamber includes one second waste liquid chamber pressure supply hole, and above-mentioned second waste liquid chamber is at least
Part is between above-mentioned first waste liquid chamber and above-mentioned second waste liquid chamber pressure supply hole.
In one embodiment, at least part of above-mentioned second channel extends along above-mentioned first direction.
In one embodiment, above-mentioned collecting chamber includes a collecting chamber pressure supply hole, and above-mentioned collecting chamber is at least partially disposed at above-mentioned
Between first waste liquid chamber and above-mentioned collecting chamber pressure supply hole.
In one embodiment, above-mentioned extraction module further includes an adsorbent, and above-mentioned adsorbent is set to above-mentioned first waste liquid chamber
Among, above-mentioned first waste liquid chamber includes one first waste liquid chamber pressure supply hole, and above-mentioned adsorbent is at least partially disposed at above-mentioned first waste liquid
Among above-mentioned first waste liquid chamber space between room pressure supply hole and above-mentioned first waste liquid chamber connecting hole.
In one embodiment, above-mentioned first waste liquid chamber pressure supply hole, above-mentioned second waste liquid chamber pressure supply hole and above-mentioned collecting chamber
Pressure supply hole is generally aligned in the same plane.
In one embodiment, above-mentioned expansion room further includes an expansion room inlet, an expansion room pressure supply hole and an expansion
Room partition, above-mentioned expansion room partition are located between above-mentioned expansion room inlet and above-mentioned expansion room pressure supply hole, above-mentioned expansion room every
Above-mentioned expansion room pressure supply hole is biased in plate bending.
In one embodiment, the present invention separately provides a kind of nucleic acid extraction method, includes the following steps.Firstly, providing an extraction
Card taking casket, above-mentioned extraction cassette include an extraction module and a liquid storage module, and above-mentioned liquid storage module is connected to above-mentioned extraction module,
Above-mentioned extraction module includes a reaction chamber, a filter membrane, a collecting chamber, one first waste liquid chamber and one second waste liquid chamber.Then, will
The mixed liquor for being sufficiently mixed a specimen and alcohol is injected above-mentioned reaction chamber from above-mentioned liquid storage module, and above-mentioned filter membrane is mixed from above-mentioned
It closes liquid and carries out nucleic acid crawl.Again, above-mentioned mixed liquor is pumped into above-mentioned first waste liquid chamber.Then, make one from above-mentioned liquid storage module
One cleaning solution passes through above-mentioned reaction chamber and above-mentioned filter membrane.Again, above-mentioned first cleaning solution is pumped into above-mentioned first waste liquid chamber.
In one embodiment, above-mentioned nucleic acid extraction method, further includes following step.Firstly, making from above-mentioned liquid storage module
One second cleaning solution passes through above-mentioned reaction chamber and above-mentioned filter membrane.Then, above-mentioned second cleaning solution is pumped into above-mentioned second waste liquid
Room.Again, so that an eluant, eluent is passed through above-mentioned reaction chamber into above-mentioned from above-mentioned liquid storage module, and stand.Then, useless with above-mentioned second
The above-mentioned second waste liquid chamber pressure supply hole of liquid chamber provides positive pressure with the chamber inlet that reacts of reaction chamber, and with the above-mentioned receipts of above-mentioned collecting chamber
Collect room pressure supply hole and negative pressure is provided, above-mentioned collecting chamber will be moved into containing the above-mentioned eluant, eluent of nucleic acid.
Using the extraction module of the embodiment of the present invention, since reaction chamber and waste liquid chamber are integrated among extraction module,
Therefore the volume of extraction cassette can be reduced, and saves cost.In particular, passing through runner, reaction chamber, collecting chamber, the first waste liquid chamber
And second waste liquid chamber configuration and pressure supply, can control waste liquid and flow into the first waste liquid chamber and the second waste liquid chamber,
And it controls the above-mentioned eluant, eluent containing nucleic acid and flows into collecting chamber, and avoid waste liquor contamination reaction chamber and collecting chamber.
Detailed description of the invention
Figure 1A shows the constitutional diagram of the extraction cassette of the embodiment of the present invention.
Figure 1B shows the explosive view of the extraction cassette of the embodiment of the present invention.
Fig. 2 shows the thin portion structure of the extraction module of the embodiment of the present invention.
Fig. 3 A, Fig. 3 B, Fig. 3 C, Fig. 3 D, Fig. 3 E, Fig. 3 F, Fig. 3 G, Fig. 3 H, Fig. 3 I, Fig. 3 J show the storage of the embodiment of the present invention
The operation steps of liquid module.
Fig. 4 shows the flow chart of the nucleic acid extraction method of the embodiment of the present invention.
Appended drawing reference is as follows:
C~extraction cassette
1~extraction module
11~reaction chamber
111~filter membrane
The outlet of 112~reaction chamber
113~taper portion
114~reaction chamber inlet
115~reaction chamber connection breach
12~collecting chamber
121~collecting chamber pressure supply hole
122~collecting chamber inclined-plane
The outlet of 123~collecting chamber
13~the first waste liquid chambers
131~the first waste liquid chamber connecting holes
132~the first waste liquid chamber pressure supply holes
14~the second waste liquid chambers
141~the second waste liquid chamber connecting holes
142~the second waste liquid chamber pressure supply holes
15~first passage
151~third channel
152~fourth lane
16~second channel
161~barricade
162~bending part
17~adsorbent
18~expand room
181~expand room inlet
182~expand room pressure supply mouth
183~expand room partition
19~extraction module body
2~liquid storage module
282~the first cleaning solutions
283~the second cleaning solutions
284~eluant, eluent
286~mixed liquor
3~sampling module
4~connectivity module
S11, S12, S13, S14, S15, S16, S17, S18, S19~step
Z~first direction
Specific embodiment
Figure 1A shows the constitutional diagram of the extraction cassette C of the embodiment of the present invention.Figure 1B shows the extraction card of the embodiment of the present invention
The explosive view of casket C.Please arrange in pairs or groups A, Figure 1B referring to Fig.1, and extraction cassette C includes extraction a module 1, a liquid storage module 2, a sampling mold
The components such as block 3, connectivity module 4.Extraction cassette C is suitable for being placed among an analyzer.Above-mentioned analyzer includes one first air pressure
Supply module, one second air pressure supply module and a detection module and, above-mentioned first air pressure supply module and above-mentioned
Two air pressure supply modules provide air pressure to extraction cassette C, to control the liquid flowing in extraction cassette C.Above-mentioned detection module pair
Extraction cassette C is heated, is radiated, and is tested and analyzed to the specimen in extraction cassette C.
Figure 1B is please referred to, liquid storage module 2 passes through the connection extraction module 1 of connectivity module 4.Fig. 2 shows the embodiment of the present invention
Extract the thin portion structure of module 1.Referring to Fig. 2, extraction module 1 includes that an extraction module body 19, one expands room 18, a reaction chamber
11, a filter membrane 111, a collecting chamber 12, one first waste liquid chamber 13 and one second waste liquid chamber 14.Expand room 18 and is formed in extraction mould
Block body 19.Reaction chamber 11 is formed in extraction module body 19, and reaction chamber 11 goes out including a reaction chamber inlet 114, a reaction chamber
Mouth 112 and a reaction chamber connection breach 115, wherein expand room 18 and connect reaction chamber connection breach 115, reaction chamber connection lacks
Mouth 115 is located between reaction chamber inlet 114 and reaction chamber outlet 112.Filter membrane 111 is set to reaction chamber 11 and corresponding reaction chamber outlet
112.Collecting chamber 12 is formed in extraction module body 19, and is connected to reaction chamber outlet 112.First waste liquid chamber 13 is formed in extraction mould
Block body 19, the first waste liquid chamber 13 are connected to reaction chamber outlet 112.Second waste liquid chamber 14, which is formed in, extracts module body 19, and second
Waste liquid chamber 14 is connected to reaction chamber outlet 112.
Referring to figure 2., expanding room 18 further includes that an expansion room inlet 181, one expands room pressure supply hole 182 and an expansion
Room partition 183 expands room partition 183 and is located at expansion room inlet 181 and expands between room pressure supply hole 182, expands room partition 183
Bending is biased to expand room pressure supply hole 182.The design for expanding room partition 183, which prevents from entering via expansion room inlet 181, expands room
Room pressure supply hole 182 is expanded in 18 liquid pollution.
In one embodiment, reaction chamber 11 includes a taper portion 113, and filter membrane 111 is set to taper portion 113, reaction chamber outlet
112 are formed in the end of taper portion 113.In one embodiment, extraction module 1 further includes that a first passage 15 and one second is logical
Road 16, first passage 15 are connected to reaction chamber outlet 112 and the first waste liquid chamber 13 and the second waste liquid chamber 14, and second channel 16 is connected to
Reaction chamber outlet 112 and collecting chamber 12.
In one embodiment, first passage 15 and above-mentioned second channel 16 are in 112 intersection of reaction chamber outlet, second channel
A barricade 161 is formed in 16, barricade 161 is formed in the end of second channel 16 and adjacent reaction chamber outlet 112.Implement one
In example, extraction module 1 further includes a third channel 151 and fourth lane 152, third channel 151 connect first passage 15 with
And first waste liquid chamber 13, fourth lane 152 connect first passage 15 and the second waste liquid chamber 14.In one embodiment, first is useless
Liquid chamber 13 includes one first waste liquid chamber connecting hole 131, and the second waste liquid chamber 14 includes one second waste liquid chamber connecting hole 141, and third is logical
Road 151 connects first passage 15 and the first waste liquid chamber connecting hole 131, and fourth lane 152 connects first passage 15 and second
Waste liquid chamber connecting hole 141, the second waste liquid chamber 14 are at least partially disposed at the first waste liquid chamber connecting hole 131 and the second waste liquid chamber connecting hole
Between 141.In one embodiment, at least part of fourth lane 152 extends along a first direction Z, and first direction Z is far from first
Waste liquid chamber 13, it is disclosed above to be not limiting as the present invention.
In one embodiment, the second waste liquid chamber 14 includes one second waste liquid chamber pressure supply hole 142, the second waste liquid chamber 14 at least portion
Quartile is between the first waste liquid chamber 13 and the second waste liquid chamber pressure supply hole 142.In one embodiment, at least part of second channel 16
Extend along first direction Z, it is disclosed above to be not limiting as the present invention.In one embodiment, collecting chamber 12 includes a collecting chamber pressure supply
Hole 121, collecting chamber 12 are at least partially disposed between the first waste liquid chamber 13 and collecting chamber pressure supply hole 121.
In one embodiment, extraction module 1 further includes an adsorbent 17, and adsorbent 17 is set among the first waste liquid chamber 13,
First waste liquid chamber 13 includes one first waste liquid chamber pressure supply hole 132, and adsorbent 17 is at least partially disposed at the first waste liquid chamber pressure supply hole 132
Among 13 space of the first waste liquid chamber between the first waste liquid chamber connecting hole 131.In one embodiment, adsorbent 17 is, for example, one
Sponge.In one embodiment, the first waste liquid chamber pressure supply hole 132, the second waste liquid chamber pressure supply hole 142 and collecting chamber pressure supply hole 121
It is generally aligned in the same plane.
Collocation shows the operation steps of the liquid storage module 2 of the embodiment of the present invention referring to Fig. 2, Fig. 3 A~Fig. 3 J.Referring to figure
3A, firstly, the mixed liquor 286 for being sufficiently mixed a specimen and alcohol is injected from liquid storage module 2 by expanding room inlet 181
Expand room 18 and reaction chamber 11, filter membrane 111 carries out nucleic acid crawl from mixed liquor 286.Then, useless by first referring to Fig. 3 B
Liquid chamber pressure supply hole 132 provides negative pressure (< -10Kpa) so that mixed liquor 286 is pumped into the first waste liquid chamber 13.Again, referring to Fig. 3 C, from storage
Liquid module 2, which passes through one first cleaning solution, 282 gradation, to expand room inlet 181, expands room 18, reaction chamber 11 and filter membrane 111,
To adjust the salt density and pH value on filter membrane 111.Then, referring to Fig. 3 D, negative pressure is provided by the first waste liquid chamber pressure supply hole 132
(< -10Kpa) the first cleaning solution 282 is pumped into the first waste liquid chamber 13.Again, referring to Fig. 3 E, from liquid storage module 2 by the one of part
Second cleaning solution 283 is by expanding room inlet 181, expanding room 18, reaction chamber 11 and filter membrane 111, to adjust on filter membrane 111
Salt density and pH value.Then, referring to Fig. 3 F, negative pressure (< -10Kpa) is provided with by the by the first waste liquid chamber pressure supply hole 132
Two cleaning solutions 283 are pumped into the first waste liquid chamber 13.Again, referring to Fig. 3 G, from liquid storage module 2 by second cleaning solution 283 of another part
By expanding room inlet 181, expanding room 18, reaction chamber 11 and filter membrane 111, with adjust the salt density on filter membrane 111 with
PH value.Then, referring to Fig. 3 H, negative pressure (< -10Kpa) is provided with by the second cleaning solution 283 by the second waste liquid chamber pressure supply hole 142
It is pumped into the second waste liquid chamber 14.Again, referring to Fig. 3 I, from liquid storage module 2 by an eluant, eluent 284 via the reaction injection warp of chamber inlet 114
Reaction chamber 11 is crossed, and stands 3 minutes, so that the nucleic acid on filter membrane 111 is detached from.Then, referring to Fig. 3 J, by applying a big positive pressure
Eluant, eluent 284 containing nucleic acid is moved into collecting chamber 12 by (45kpa).Collocation referring to Fig. 2, Fig. 3 J the step of in, second is useless
Liquid chamber pressure supply hole 142 provides small positive pressure, to prevent the eluant, eluent 284 containing nucleic acid from entering first passage 15.In one embodiment,
Above-mentioned big positive pressure (45kpa) is provided by liquid storage module 2.
In the above-mentioned Fig. 3 D, Fig. 3 F the step of, the cleaning time of the first cleaning solution 282 and the second cleaning solution 283 can be with
It is disclosed above to be not limiting as the present invention for once or more.In one embodiment, the first cleaning solution 282 is to neutralize pH
Value, the second cleaning solution 283 is for cleaning protein and organic impurities.
In the above-described embodiments, the design for expanding room 18 prevents mixed liquor 286, the first cleaning solution 282 and the second cleaning
The liquid level of liquid 283 touches reaction chamber inlet 114, to prevent eluant, eluent 284 from being polluted.
It please arrange in pairs or groups referring to Fig. 2, in the above-described embodiments, the design of barricade 161 can prevent mixed liquor 286, the first cleaning solution
282 and second cleaning solution 283 enter collecting chamber 12.In one embodiment, second channel 16 has a bending part 162, bending
The design in portion 162 can make the eluant, eluent 284 containing nucleic acid adequately enter collecting chamber 12.In one embodiment, collecting chamber 12 is gone back
Including a collecting chamber inclined-plane 122 and collecting chamber outlet 123, collecting chamber inclined-plane 122 connects collecting chamber outlet 123, collecting chamber
The design on inclined-plane 122 can make the eluant, eluent 284 containing nucleic acid adequately via 123 outflow of collecting chamber outlet (to sampling module 3,
Arrange in pairs or groups B referring to Fig.1).In one embodiment, bending part 162 is towards collecting chamber inclined-plane 122.
Collocation referring to Fig. 2, Fig. 3 H the step of in, the second cleaning solution 283 of last part is drawn into the second waste liquid chamber
14.Waste liquid (above-mentioned second cleaning solution 283) in second waste liquid chamber 14 is most clean waste liquid.In Fig. 3 J the step of, second
Waste liquid chamber pressure supply hole 142 provides small positive pressure, to prevent the eluant, eluent 284 containing nucleic acid from entering first passage 1.Due to the second waste liquid
Waste liquid (above-mentioned second cleaning solution 283) in room 14 is most clean waste liquid, therefore is provided in the second waste liquid chamber pressure supply hole 142 small
In the case where positive pressure, the gas in the second waste liquid chamber 14 will drop to the pollution of the eluant, eluent 284 containing nucleic acid minimum.
Collocation referring to Fig. 2, Fig. 3 F the step of in, the waste liquid in the first waste liquid chamber 13 is into contact with adsorbent 17, absorption
Body 17 can eliminate the bubble of waste liquid, and the waste liquid in the first waste liquid chamber 13 is avoided to overflow from above-mentioned first waste liquid chamber pressure supply hole 132.?
In one embodiment, filter membrane 111 can be silicon filter membrane or the filter membrane of other materials.In one embodiment, extraction module 1 is integrated into
Type.
Referring to Fig. 4, in one embodiment, the present invention separately provides a kind of nucleic acid extraction method, includes the following steps.Firstly,
An extraction cassette is provided, above-mentioned extraction cassette includes an extraction module and a liquid storage module, and above-mentioned liquid storage module connection is above-mentioned
Module is extracted, above-mentioned extraction module includes a reaction chamber, a filter membrane, a collecting chamber, one first waste liquid chamber and one second waste liquid
Room (S11).Then, the mixed liquor for being sufficiently mixed a specimen and alcohol is injected into above-mentioned reaction chamber from above-mentioned liquid storage module,
Above-mentioned filter membrane carries out nucleic acid crawl (S12) from above-mentioned mixed liquor.Furthermore above-mentioned mixed liquor is pumped into above-mentioned first waste liquid chamber
(S13).Then, make one first cleaning solution by above-mentioned reaction chamber and above-mentioned filter membrane (S14) from above-mentioned liquid storage module.Furthermore
Above-mentioned first cleaning solution is pumped into above-mentioned first waste liquid chamber (S15).Then, pass through one second cleaning solution from above-mentioned liquid storage module
Above-mentioned reaction chamber and above-mentioned filter membrane (S16).Then, above-mentioned second cleaning solution is pumped into above-mentioned second waste liquid chamber (S17).Again
Person enters an eluant, eluent from above-mentioned liquid storage module above-mentioned by above-mentioned reaction chamber, and stands (S18).Then, with above-mentioned second
The above-mentioned second waste liquid chamber pressure supply hole of waste liquid chamber provides positive pressure, and is provided and born with the above-mentioned collecting chamber pressure supply hole of above-mentioned collecting chamber
Pressure will move into above-mentioned collecting chamber (S19) containing the above-mentioned eluant, eluent of nucleic acid.
Using the extraction module of the embodiment of the present invention, since reaction chamber and waste liquid chamber are integrated among extraction module,
Therefore the volume of extraction cassette can be reduced, and saves cost.In particular, passing through runner, reaction chamber, collecting chamber, the first waste liquid chamber
And second waste liquid chamber configuration and pressure supply, can control waste liquid and flow into the first waste liquid chamber and the second waste liquid chamber,
And it controls the above-mentioned eluant, eluent containing nucleic acid and flows into collecting chamber, and avoid waste liquor contamination reaction chamber and collecting chamber.
Although the present invention is disclosed as above with specific preferred embodiment, however, it is not to limit the invention, Ren Heben
The those of ordinary skill in field still can make some changes and embellishment without departing from the spirit and scope of the present invention, therefore this
The protection scope of invention is subject to view appended claims institute defender.
Claims (15)
1. a kind of extraction cassette, comprising:
One liquid storage module；And
One extraction module, is connected to above-mentioned liquid storage module, comprising:
One extraction module body；
One expands room, is formed in above-mentioned extraction module body；
One reaction chamber is formed in above-mentioned extraction module body, including a reaction chamber inlet, reaction chamber outlet and a reaction chamber
Connection breach, wherein above-mentioned expansion room connects above-mentioned reaction chamber connection breach, and above-mentioned reaction chamber connection breach are located at above-mentioned reaction
Between chamber inlet and the outlet of above-mentioned reaction chamber；
One filter membrane is set to above-mentioned reaction chamber and corresponding above-mentioned reaction chamber outlet；
One collecting chamber is formed in above-mentioned extraction module body, and is connected to above-mentioned reaction chamber outlet；
One first waste liquid chamber, is formed in above-mentioned extraction module body, and above-mentioned first waste liquid chamber is connected to above-mentioned reaction chamber outlet；And
One second waste liquid chamber, is formed in above-mentioned extraction module body, and above-mentioned second waste liquid chamber is connected to above-mentioned reaction chamber outlet.
2. extraction cassette as described in claim 1, wherein above-mentioned reaction chamber includes a taper portion, above-mentioned filter membrane is set to above-mentioned cone
Shape portion, above-mentioned reaction chamber outlet are formed in the end of above-mentioned taper portion.
3. extraction cassette as described in claim 1, wherein above-mentioned extraction module further includes that a first passage and one second are logical
Road, the above-mentioned above-mentioned reaction chamber outlet of first passage connection and above-mentioned first waste liquid chamber and above-mentioned second waste liquid chamber, above-mentioned second
Channel is connected to above-mentioned reaction chamber outlet and above-mentioned collecting chamber.
4. extraction cassette as claimed in claim 3, wherein above-mentioned first passage and above-mentioned second channel are in above-mentioned reaction chamber
Outlet is intersected, and a barricade is formed in above-mentioned second channel, and above-mentioned barricade is formed in the end of above-mentioned second channel and adjoining
State reaction chamber outlet.
5. extraction cassette as claimed in claim 3, wherein above-mentioned extraction module further includes a third channel and a four-way
Road, above-mentioned third channel connect above-mentioned first passage and above-mentioned first waste liquid chamber, and above-mentioned fourth lane connection above-mentioned first is logical
Road and above-mentioned second waste liquid chamber.
6. extraction cassette as claimed in claim 5, wherein above-mentioned first waste liquid chamber includes one first waste liquid chamber connecting hole, it is above-mentioned
Second waste liquid chamber includes one second waste liquid chamber connecting hole, and above-mentioned third channel connects above-mentioned first passage and above-mentioned first waste liquid
Room connecting hole, above-mentioned fourth lane connect above-mentioned first passage and above-mentioned second waste liquid chamber connecting hole, above-mentioned second waste liquid chamber
It is at least partially disposed between above-mentioned first waste liquid chamber connecting hole and above-mentioned second waste liquid chamber connecting hole.
7. extraction cassette, the above-mentioned fourth lane of wherein at least part extend along a first direction as claimed in claim 6, on
First direction is stated far from above-mentioned first waste liquid chamber.
8. cassette is extracted as claimed in claim 6, wherein above-mentioned second waste liquid chamber includes one second waste liquid chamber pressure supply hole, it is above-mentioned
Second waste liquid chamber is at least partially disposed between above-mentioned first waste liquid chamber and above-mentioned second waste liquid chamber pressure supply hole.
9. extraction cassette, the above-mentioned second channel of wherein at least part extend along above-mentioned first direction as claimed in claim 7.
10. extraction cassette as claimed in claim 8, wherein above-mentioned collecting chamber includes a collecting chamber pressure supply hole, above-mentioned collecting chamber
It is at least partially disposed between above-mentioned first waste liquid chamber and above-mentioned collecting chamber pressure supply hole.
11. extraction cassette as claimed in claim 10, wherein above-mentioned extraction module further includes an adsorbent, above-mentioned adsorbent is set
Among above-mentioned first waste liquid chamber, above-mentioned first waste liquid chamber includes one first waste liquid chamber pressure supply hole, and above-mentioned adsorbent is at least partly
Among above-mentioned first waste liquid chamber space between above-mentioned first waste liquid chamber pressure supply hole and above-mentioned first waste liquid chamber connecting hole.
12. extraction cassette as claimed in claim 11, wherein above-mentioned first waste liquid chamber pressure supply hole, above-mentioned second waste liquid chamber pressure supply
Hole and above-mentioned collecting chamber pressure supply hole are generally aligned in the same plane.
13. extraction cassette as described in claim 1, wherein above-mentioned expansion room further includes an expansion room inlet, an expansion room
Pressure supply hole and one expand room partition, above-mentioned expansion room partition be located at above-mentioned expansion room inlet and above-mentioned expansion room pressure supply hole it
Between, above-mentioned expansion room pressure supply hole is biased in partition bending in above-mentioned expansion room.
14. a kind of nucleic acid extraction method, comprising:
An extraction cassette is provided, above-mentioned extraction cassette includes an extraction module and a liquid storage module, above-mentioned liquid storage module connection
Above-mentioned extraction module, above-mentioned extraction module include a reaction chamber, a filter membrane, a collecting chamber, one first waste liquid chamber and one second
Waste liquid chamber；
A mixed liquor of a specimen and alcohol will be sufficiently mixed and injected above-mentioned reaction chamber from above-mentioned liquid storage module, above-mentioned filter membrane from
Above-mentioned mixed liquor carries out nucleic acid crawl；
Above-mentioned mixed liquor is pumped into above-mentioned first waste liquid chamber；
Make one first cleaning solution by above-mentioned reaction chamber and above-mentioned filter membrane from above-mentioned liquid storage module；And
Above-mentioned first cleaning solution is pumped into above-mentioned first waste liquid chamber.
15. nucleic acid extraction method as claimed in claim 14, further include:
Make one second cleaning solution by above-mentioned reaction chamber and above-mentioned filter membrane from above-mentioned liquid storage module；
Above-mentioned second cleaning solution is pumped into above-mentioned second waste liquid chamber；
Enter an eluant, eluent from above-mentioned liquid storage module above-mentioned by above-mentioned reaction chamber, and stands；And
Positive pressure is provided with one second waste liquid chamber pressure supply hole of above-mentioned second waste liquid chamber, and with a collecting chamber pressure supply of above-mentioned collecting chamber
Hole provides negative pressure, will move into above-mentioned collecting chamber containing the above-mentioned eluant, eluent of nucleic acid.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16/229,685 US11207681B2 (en) | 2015-07-17 | 2018-12-21 | Method for extracting nucleic acid and extraction cassette thereof |
| ES18215536T ES2895753T3 (en) | 2017-12-26 | 2018-12-21 | Nucleic acid extraction method and extraction cassette. |
| EP18215536.6A EP3505253B1 (en) | 2017-12-26 | 2018-12-21 | Method for extracting nucleic acid and extraction cassette thereof |
| JP2018240434A JP6861199B2 (en) | 2017-12-26 | 2018-12-24 | Nucleic acid extraction method and its extraction cassette |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762610444P | 2017-12-26 | 2017-12-26 | |
| US62/610,444 | 2017-12-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110004141A true CN110004141A (en) | 2019-07-12 |
Family
ID=67076194
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811532891.XA Active CN109966776B (en) | 2015-07-17 | 2018-12-14 | Nucleic acid extraction method and extraction cartridge thereof |
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| WO2020256005A1 (en) | 2019-06-21 | 2020-12-24 | 住友ベークライト株式会社 | Thermosetting resin composition, resin sheet, and metal base substrate |
| CN112891986B (en) * | 2021-01-30 | 2022-05-03 | 哈尔滨工业大学 | Integrated closed nucleic acid automatic extraction device |
| TWI792671B (en) * | 2021-11-11 | 2023-02-11 | 台達電子工業股份有限公司 | Aerosol filter and nucleic acid extraction cassette |
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| TW201932590A (en) | 2019-08-16 |
| CN109966776B (en) | 2021-07-13 |
| JP6682611B2 (en) | 2020-04-15 |
| TWI685564B (en) | 2020-02-21 |
| TWI685565B (en) | 2020-02-21 |
| JP2019115337A (en) | 2019-07-18 |
| TW201938785A (en) | 2019-10-01 |
| JP6861199B2 (en) | 2021-04-21 |
| CN109966776A (en) | 2019-07-05 |
| ES2895753T3 (en) | 2022-02-22 |
| JP2019115338A (en) | 2019-07-18 |
| ES2869703T3 (en) | 2021-10-25 |
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