CN1099558A - Phytohormone combination method and phytohormone thereof - Google Patents
Phytohormone combination method and phytohormone thereof Download PDFInfo
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- CN1099558A CN1099558A CN 93116693 CN93116693A CN1099558A CN 1099558 A CN1099558 A CN 1099558A CN 93116693 CN93116693 CN 93116693 CN 93116693 A CN93116693 A CN 93116693A CN 1099558 A CN1099558 A CN 1099558A
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Abstract
The extensively used growth substances, gibberellins and auxetics are compounded through electrochemical, acidifying and esterifying etc. treatments by the present invention. It overcomes such shortages of the above mentioned three kinds of phytohormones, as unstable property of their mixture, unitary function and short storage period. The productive experiments showed that the compounded phytohormone (808, bailingbao) possesses of such obvious results to the vegetables, crops and fruit trees etc. plants as promoting growth, increasing setting, improving the quality and incereasing the yield.
Description
The present invention is specifically related to the new complex method of a kind of plant hormone and utilizes this method to prepare a kind of new composite plant hormone.
Plant hormone (or claiming plant growth regulating substance) is to be widely used respond well plant growth regulating substance on the present agricultural, and normally used plant hormone is divided into auxins, as 2.4-D, 2.4.5-T, IAA, IBA, NAA, PCPA or its analog; The gibberellin class, as gibberellic acid GA series of products, the basic element of cell division, as three major types plant growth regulator such as kinetin KT, 6-benzyl aminopurine BA adenine sulfate or zeatin ZT, the different plant hormone of these performances can be regulated root, stem, leaf, flower, really growth and the growth of plant respectively.Plant corpus itself contains the above-mentioned plant hormone of some, but can not satisfy the needs that it grows far away, needs the artificial above-mentioned exogenous hormone that adds usually.But use this three classes plant hormone simultaneously on producing at present because the back performance that is mixed is extremely unstable, can only be now with the current, only can store about 3 days under the normal temperature, in low temperature refrigerator, also can only store 1-2 week, make troubles to use.Produce the use of going up simultaneously at present and also rest on the single hormone application level, can not bring into play the synergy of plant hormone well plant hormone.
The objective of the invention is to overcome the deficiency of prior art, the new complex method of a kind of plant hormone (growth hormone, gibberellin and the basic element of cell division) is proposed, and utilize this method to prepare a kind of composite plant hormone, with solve present plant hormone be mixed the back unstable properties, storage period, the not long and less problem of regulatory function was to use above complex method to prepare a kind of new composite plant hormone simultaneously.
The present invention is achieved through the following technical solutions:
A kind of complex method of plant hormone, plant hormone in this method is usually said auxins, gibberellin class and cytokinin plant growth regulating substance, auxin substance wherein is 2.4-D, 2.4.5-T, the IAA(heteroauxin), the IBA(indolebutyric acid), the NAA(methyl) or the compound drop-proof agent of PCPA(), gibberellin is gibberellic acid GA, is preferably GA
3The basic element of cell division is the KT(cytokinin), the BA(6-benayl aminopurine), adenine sulfate or ZT(zeatin) etc., method of the present invention is that above-mentioned plant hormone is mixed into the aqueous solution by 100-5000ppm concentration, by electrification, acidifying and esterification treatment, be prepared into and mix the back stable performance, the composite plant hormone that regulatory function is complete and storage period is long then.
Described electrification is handled, and above-mentioned mixed liquor is carried out in a treating apparatus that is connected with the 50-500mA electric current, and this installs available electrophoresis apparatus or other similar device replaces.Processing time finishes up to this mixed liquor acidifying and esterification treatment process.
Described acidification, be that the mixed liquor in the electrochemical treating apparatus is added organic acid or inorganic acid solution, organic acid wherein can be chosen from formic acid, glacial acetic acid or citric acid, inorganic acid wherein can be chosen from nitric acid, hydrochloric acid, sulfuric acid or phosphoric acid, normal temperature stirs down, and PH is maintained between the 2-6.
Described esterification treatment is meant in above-mentioned mixed liquor to add lower alcohol that described alcohol can be chosen from methyl alcohol, ethanol, propane diols or butanols, adding proportion is (by weight) alcohol: acid=1-5: 1, and normal temperature stirs down, processing time 18-26 hour.
The assembly concentration of the auxins of indication of the present invention, gibberellin class and cytokinin is assembly in the 100-5000ppm scope respectively.
Utilize the composite plant hormone of above-mentioned complex method preparation, at least comprise usually said growth hormone, plant growth regulating substance such as the gibberellin and the basic element of cell division, described growth hormone can be from 2.4-D, 2.4.5-T, IAA, IBA, at least choose a kind ofly among NAA or the PCPA, described gibberellin can be chosen a kind of from the gibberellic acid series of products at least, preferably selects GA among the present invention for use
3The said basic element of cell division can be from KT, BA, adenine sulfate and ZT(zeatin) choose at least wherein a kind of, their concentration in combination solution is 100-5000ppm, the composite plant hormone of indication of the present invention is meant and contains above-mentioned three class plant hormones (at least a or several assembly of every parahormone), through electrification, the assembly mixed solution of acidifying and esterification treatment.Needs according to vegetables, fruit tree or other crops Different Nutrition, also can be after above-mentioned processing finishes, go into N(nitrogen respectively), P(phosphorus), K(potassium), B(boron), Zn(zinc), the Mo(molybdenum) etc. macronutrient or micronutrient element, also can add rare earth.
Compared with the prior art, a difficult problem such as method of the present invention can solve this three classes plant hormone preferably and mix the back stable performance, and regulatory function is complete, this plant hormone (the present invention's person: 808 plant hormones, commodity are called " lark treasured "); Store at normal temperatures and can be convenient to the peasant and use and transport, store for 2 years, its concrete assembly processing and implementation result see following embodiment and implementation result analysis for details.
Be three embodiment of the present invention below:
Embodiment one:
1, dissolving: accurately take by weighing 2.4-D10mg, GA
320mg, BA20mg is respectively with 4ml water, 4ml ethanol, 4mlKOH dissolving.
2, electrification: three kinds of solution are poured in the general electrophoresis tank, passed to the 70mA electric current, begin energising always in acidifying, esterification, acidifying, the esterification process after 30 minutes.
3, acidifying: slowly add the 10ml glacial acetic acid, stirred gently 1 hour.Transfer PH=3 with KOH.
4, esterification: add 75% propane diols 20ml, be interrupted gently under the normal temperature and stir, make reaction 18 hours.
Fixed molten with propane diols at last to 50ml, promptly obtain containing 2.4-D200ppm, GA
3400ppm, the stable composition plant hormone of BA400ppm, this plant hormone can be deposited 2 years at normal temperatures.
Embodiment two:
1, dissolving: accurately take by weighing 2.4-D100mg, GA
3100mg, BA200mg is respectively with 5ml water, 5ml ethanol, 5mlKOH dissolving.
2, electrification: three kinds of solution are poured in the general electrophoresis tank, passed to the 150mA electric current, begin acidifying, esterification after 30 minutes, energising always in acidifying, the esterification process.
3, acidifying: slowly add the 12ml glacial acetic acid, stirred gently 1 hour.Transfer PH=3 with KOH.
4, esterification: add 75% propane diols 22ml, be interrupted gently under the normal temperature and stir, make reaction 22 hours.
Fixed molten with propane diols at last to 50ml, promptly obtain containing 2.4-D2000ppm, GA
32000ppm, the stable composition plant hormone of BA4000ppm, this plant hormone can be deposited 2 years at normal temperatures.
Embodiment three:
1, dissolving: accurately take by weighing 2.4-D50mg, GA
350mg, BA50mg is respectively with 4ml water, 4ml ethanol, 4mlKOH dissolving.
2, electrification: three kinds of solution are poured in the general electrophoresis tank, passed to the 100mA electric current, begin acidifying, esterification after 30 minutes, energising always in acidifying, the esterification process.
3, acidifying: slowly add the 10ml glacial acetic acid, stirred gently 1 hour.Transfer PH=3 with KOH.
4, esterification: add 75% propane diols 20ml, be interrupted gently under the normal temperature and stir, make reaction 26 hours.
Fixed molten with propane diols at last to 50ml, promptly obtain containing 2.4-D1000ppm, GA
31000ppm, the stable composition plant hormone of BA2000ppm, this plant hormone can be deposited 2 years at normal temperatures.
Test effect example 1:
Prevent that watermelon fallen flowers result from reaching the influence to fruit and seed.Six processing, a contrast are established in test.Wherein handling four is mixtures of now with the current three kinds of hormones, handle five be compound after through the composite plant hormone of storage, processing six is compound after the composite plant hormone of 54 ℃ of storages.Be coated with or be sprayed on the watermelon ovary on the same day of blooming, established three repetitions.Result such as table 1:
Effect test 2
The composite plant hormone prevents the effect test of cotton bud drop:
Six processing are established in test, a contrast, and each handles 50 buds, and the flower bud phase handles once, and short-term is handled once.Investigate ripe cotton boll number, the result is as follows:
Handle the ripe peach of flower bud number (individual) (individual) knot peach rate (%)
CK 50 19 38
2.4-D15ppm 50 17 34
GA20ppm 50 23 46
BA20ppm 50 34 68
Mixed solution 50 47 94
Compound (not storage) 50 46 92
Compound (storage) 50 48 96
Test site: agrotechnical station, tidal land district, Shaoxing, Zhejiang Province county.（1988）
Claims (7)
1, a kind of complex method of plant hormone, plant hormone wherein comprises usually said auxins, gibberellin class and cytokinin plant growth regulating substance, it is characterized in that described plant growth regulating substance is made aqua according to a certain ratio, by electrification, treatment step such as acidifying and esterification, make and mix its stable performance of back, the composite plant hormone of the complete and extended pot life of regulatory function, auxin substance wherein is 2.4-D, 2.4.5-T, IAA (heteroauxin), IBA (indolebutyric acid), NAA (methyl) or PCPA (compound drop-proof agent), gibberellin is gibberellic acid GA, is preferably GA
3, the basic element of cell division is KT (cytokinin), BA (6-benzyl aminopurine), adenine sulfate or ZT (zeatin).
2, the method for claim 1, it is characterized in that it is with described three class plant hormones according to a certain ratio that described electrification is handled, carry out in a treating apparatus that is connected with the 50-500mA electric current, the processing time finishes up to this mixed liquor acidifying and esterification treatment process.
3, method as claimed in claim 1 or 2, it is characterized in that described acidification, be that the mixed liquor in the electrochemical treating apparatus is added organic acid or inorganic acid solution, organic acid wherein can be chosen from formic acid, glacial acetic acid or citric acid, inorganic acid wherein can be chosen from nitric acid, hydrochloric acid, sulfuric acid or phosphoric acid, normal temperature stirs down, and PH is maintained between the 2-6.
4, as claim 1 or 3 described methods, it is characterized in that, described esterification treatment, be meant in above-mentioned mixed liquor and add lower alcohol, described alcohol can be chosen from methyl alcohol, ethanol, propane diols or butanols, adding proportion is (by weight) alcohol: acid=1-5: 1, and normal temperature stirs down, processing time 18-26 hour.
5, as claim 1 or 4 described methods, its spy is, described auxin substance (solution is down together), and assembly concentration is 100-5000ppm, and gibberellin is 100-5000ppm, and the basic element of cell division is 100-5000ppm.
6, a kind of composite plant hormone, this hormone comprises usually said growth hormone at least, plant growth regulating substance such as the gibberellin and the basic element of cell division, described growth hormone can be from 2.4-D, 2.4.5-T, IAA, IBA, at least choose a kind ofly among NAA or the PCPA, choosing at least that said gibberellin can be from gibberellic acid GA is a kind of, preferably GA
3The said basic element of cell division can be from KT, BA, adenine sulfate and ZT(zeatin) choose at least wherein a kind of, their concentration in combination solution is 100-5000ppm, the composite plant hormone of indication of the present invention is meant and contains above-mentioned three class plant hormones (every class is at least a kind of or several compounds is formed) through electrification, the assembly mixed solution of acidifying and esterification treatment.
7, plant hormone as claimed in claim 6, it is characterized in that, needs according to vegetables, fruit tree or other crops Different Nutrition, also can be in above-mentioned solution, add N(nitrogen respectively), P(phosphorus), K(potassium), B(boron), Zn(zinc) or the Mo(molybdenum) etc. macronutrient or micronutrient element, also can add rare earth.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 93116693 CN1099558A (en) | 1993-08-30 | 1993-08-30 | Phytohormone combination method and phytohormone thereof |
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| CN 93116693 CN1099558A (en) | 1993-08-30 | 1993-08-30 | Phytohormone combination method and phytohormone thereof |
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| CN1099558A true CN1099558A (en) | 1995-03-08 |
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| CN 93116693 Pending CN1099558A (en) | 1993-08-30 | 1993-08-30 | Phytohormone combination method and phytohormone thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1062112C (en) * | 1997-09-05 | 2001-02-21 | 季玉璞 | Effective fruit-setting agent for field pumpkin |
| US7071214B2 (en) * | 1997-02-07 | 2006-07-04 | Emisphere Technologies, Inc. | Compounds and compositions for delivering active agents |
| CN100381058C (en) * | 2006-09-12 | 2008-04-16 | 中国农业大学 | Seed treatment in use for accelerating germination of seed |
| CN100393216C (en) * | 2005-07-15 | 2008-06-11 | 中国科学院长春应用化学研究所 | Plant growth regulating composition for seed germination |
| CN100399899C (en) * | 2005-07-15 | 2008-07-09 | 中国科学院长春应用化学研究所 | Plant growth regulating composition for rooting |
| CN101361483B (en) * | 2008-10-14 | 2011-07-27 | 中国农业科学院作物科学研究所 | Corn low temperature resistance production-increasing regulator and preparation method thereof |
| CN101438710B (en) * | 2008-12-25 | 2012-05-09 | 福建省农业科学院果树研究所 | Method for saving frozen loquat fruit |
| CN103190416A (en) * | 2013-04-10 | 2013-07-10 | 新疆农业大学 | Method for adjusting Fuji apple shapes by spraying exogenous hormones |
| CN104585194A (en) * | 2014-12-31 | 2015-05-06 | 安徽农业大学 | Pregnancy-prompting agent for zizania aquatica and method for increasing pregnancy rate and quality of zizania aquatica |
| CN104920394A (en) * | 2015-05-26 | 2015-09-23 | 深圳市易普乐生物科技有限公司 | Composite nutrient solution for adjusting growth of crops, preparation method and application thereof |
| CN108293691A (en) * | 2017-09-08 | 2018-07-20 | 广西深禾丰农业科技发展有限公司 | A kind of cultural method of aspermous watermelon |
| CN109452297A (en) * | 2018-11-22 | 2019-03-12 | 佛山科学技术学院 | A kind of composite plant growth regulator and its application in raising autumn eggplant percentage of seedgermination |
| CN111887251A (en) * | 2020-08-26 | 2020-11-06 | 吉林省林业科学研究院 | Mixed hormone beneficial to growth of Korean pine |
-
1993
- 1993-08-30 CN CN 93116693 patent/CN1099558A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7071214B2 (en) * | 1997-02-07 | 2006-07-04 | Emisphere Technologies, Inc. | Compounds and compositions for delivering active agents |
| CN1062112C (en) * | 1997-09-05 | 2001-02-21 | 季玉璞 | Effective fruit-setting agent for field pumpkin |
| CN100393216C (en) * | 2005-07-15 | 2008-06-11 | 中国科学院长春应用化学研究所 | Plant growth regulating composition for seed germination |
| CN100399899C (en) * | 2005-07-15 | 2008-07-09 | 中国科学院长春应用化学研究所 | Plant growth regulating composition for rooting |
| CN100381058C (en) * | 2006-09-12 | 2008-04-16 | 中国农业大学 | Seed treatment in use for accelerating germination of seed |
| CN101361483B (en) * | 2008-10-14 | 2011-07-27 | 中国农业科学院作物科学研究所 | Corn low temperature resistance production-increasing regulator and preparation method thereof |
| CN101438710B (en) * | 2008-12-25 | 2012-05-09 | 福建省农业科学院果树研究所 | Method for saving frozen loquat fruit |
| CN103190416A (en) * | 2013-04-10 | 2013-07-10 | 新疆农业大学 | Method for adjusting Fuji apple shapes by spraying exogenous hormones |
| CN104585194A (en) * | 2014-12-31 | 2015-05-06 | 安徽农业大学 | Pregnancy-prompting agent for zizania aquatica and method for increasing pregnancy rate and quality of zizania aquatica |
| CN104920394A (en) * | 2015-05-26 | 2015-09-23 | 深圳市易普乐生物科技有限公司 | Composite nutrient solution for adjusting growth of crops, preparation method and application thereof |
| CN108293691A (en) * | 2017-09-08 | 2018-07-20 | 广西深禾丰农业科技发展有限公司 | A kind of cultural method of aspermous watermelon |
| CN109452297A (en) * | 2018-11-22 | 2019-03-12 | 佛山科学技术学院 | A kind of composite plant growth regulator and its application in raising autumn eggplant percentage of seedgermination |
| CN111887251A (en) * | 2020-08-26 | 2020-11-06 | 吉林省林业科学研究院 | Mixed hormone beneficial to growth of Korean pine |
| CN111887251B (en) * | 2020-08-26 | 2021-08-31 | 吉林省林业科学研究院 | Mixed hormone beneficial to growth of Korean pine |
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