CN109911425B - Pulmonary tuberculosis detection kit and application method thereof - Google Patents
Pulmonary tuberculosis detection kit and application method thereof Download PDFInfo
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- CN109911425B CN109911425B CN201910088206.7A CN201910088206A CN109911425B CN 109911425 B CN109911425 B CN 109911425B CN 201910088206 A CN201910088206 A CN 201910088206A CN 109911425 B CN109911425 B CN 109911425B
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Abstract
The invention discloses a pulmonary tuberculosis detection kit and application thereof, wherein the kit comprises a kit body and a cover body, the cover body is arranged above the kit body, the interior of the kit body is divided into a material area and a reagent area by a partition plate, a step-shaped partition plate is arranged in the material area, a sample membrane area, a colloidal gold pad area, an acid cellulose membrane area and a tool area are sequentially arranged on the step-shaped partition plate from top to bottom, a workbench is arranged on the kit body below the step-shaped partition plate, and the workbench is obliquely arranged; the detection kit can be used for rapidly completing preparation work of colloidal gold detection in cooperation with the step-shaped partition plate, has repeatable operability, can detect whether tuberculosis antigens exist in a serum sample by using a double-antibody sandwich method, has high specificity, high sensitivity and high accuracy in cooperation with the inclined arrangement of a workbench, can rapidly screen out positive samples with tuberculosis antigen serum, can rapidly and conveniently assist in diagnosing the pulmonary tuberculosis, has detection time of 3-5min, and has wide practicability in the field of disease detection.
Description
Technical Field
The invention mainly relates to the technical field of immunodiagnosis, in particular to the technical field of an immunodiagnosis kit for tuberculosis.
Background
The world health organization publishes a '2018 global tuberculosis report' on 18 th 9 th 2018 th, and shows that the number of tuberculosis latent infection groups around the world in 2017 is about 17 hundred million, and the latent infection rate is 23%. The worldwide new tuberculosis patients are about 1000 million, the tuberculosis incidence rate is 133/10 million, wherein children patients less than 15 years old and HIV infected patients respectively account for 10 percent and 9 percent of the new tuberculosis patients; the number of new patients in 30 tuberculosis-burdened countries accounts for 87.2% of the world; new patients in india (27.4%), china (8.9%), indonesia (8.4%) and philippines (5.8%) make up about 50% of the world. The number of new patients with the estimated tuberculosis in China is 88.9 ten thousand, the estimated tuberculosis incidence rate is 63/10 ten thousand, and the estimated tuberculosis incidence rate is ranked 28 in 30 tuberculosis high-burden countries. The detection of pulmonary tuberculosis is essential.
Tuberculosis (pulmonytuberculosispdb) is an ancient infectious disease, an infectious disease of the lung caused by mycobacterium tuberculosis, and a disease seriously threatening human health. The infectious agent of Mycobacterium tuberculosis (hereinafter referred to as tubercle bacillus) is mainly the tuberculosis patient who is infected by bacteria, and is spread through respiratory tract. The tubercle bacillus infected by healthy people does not always attack, but only when the immunity of the organism is reduced. The pulmonary tuberculosis can be effectively prevented by detecting in time.
The colloidal gold is a common marking technology, is a novel immune marking technology which applies the colloidal gold as a tracer marker to antigen and antibody, and has unique advantages. Have been widely used in various biological studies in recent years. The immunoblotting technique used in clinical practice almost exclusively uses its markers. And can be used in flow, electron microscope, immunity, molecular biology and biochip.
The traditional tuberculosis detection needs imaging detection matched with other detection, and the detection process is long and inaccurate. For example, smear examination of tubercle sputum: the sensitivity is poor, the positive rate is low, the positive can confirm the tuberculosis, the negative can not exclude or confirm the tuberculosis, the repeated examination is needed for many times, and the tubercle bacillus can not be distinguished from other nontuberculous mycobacteria; culturing mycobacterium tuberculosis: the required period is long, usually 2-4 weeks, and non-infectious disease hospitals can not develop, positive can confirm the diagnosis of the pulmonary tuberculosis, negative can not exclude or determine the pulmonary tuberculosis, and multiple repeated examinations are needed and the comprehensive judgment is carried out by combining the clinical symptoms, physical signs and PPD results; tuberculin test (PPD): the specificity and the sensitivity are not high, and the strong positive person has prompt value but can not be used independently; tuberculosis infected T cell spot assay (T-spot. tb): the susceptibility and the specificity of detection in patients with tuberculosis including bacteria-negative tuberculosis and extrapulmonary tuberculosis are high, and the detection of the suspected tuberculosis patients with bacteria-negative tuberculosis and extrapulmonary tuberculosis has great help significance for early diagnosis, but has great disputes in children and other immunodeficiency patients and in the aspect of judging the clinical prognosis of tuberculosis at present; bronchofiberscope and other endoscopy, and bronchofiberscope alveolar lavage PCR for tuberculosis DNA: invasive examination and less use; needle biopsy under CT guidance: for atypical tuberculosis around the lung, puncture biopsy can be conducted under CT guidance, pathological examination is conducted, sensitivity and specificity are high, and puncture surgery is needed for the biopsy.
Disclosure of Invention
The invention aims to provide a pulmonary tuberculosis detection kit and application thereof, aiming at solving the technical problems that the prior traditional pulmonary tuberculosis detection needs imaging detection matched with other detection, the detection process is longer and inaccurate, the prior colloidal gold detection kit is mostly disposable, and no reusable colloidal gold test paper is used for detecting the pulmonary tuberculosis, the detection kit can be used for rapidly completing the preparation work of the colloidal gold detection by matching with a step-shaped baffle plate, has repeatable operability, detects whether a tuberculosis antigen exists in a serum sample by using a double-antibody sandwich method, has high specificity and high sensitivity by matching with the inclined arrangement of a worktable, the kit has high-accuracy detection performance, can quickly screen out a positive sample with tuberculosis antigen serum, can quickly and conveniently assist in diagnosing the pulmonary tuberculosis, has the detection time of 3-5min, and has wide practicability and development value in the field of disease detection.
The invention is realized by the following technical scheme:
the invention also provides a kit for detecting the pulmonary tuberculosis, which comprises a box body and a cover body, wherein the cover body is arranged above the box body, the box body is divided into a material area and a reagent area by a partition plate, a step-shaped partition plate is arranged in the material area, a sample membrane area, a colloidal gold pad area, an acid cellulose membrane area and a tool area are sequentially arranged on the step-shaped partition plate from top to bottom, a workbench is arranged on the box body below the step-shaped partition plate, the workbench is obliquely arranged, a partition plate III is arranged in the reagent area, a sealing liquid bottle, an anti-gold-labeled antibody bottle, a washing liquid bottle and an antibody bottle are arranged on the partition plate III.
According to the invention, a groove is formed in the workbench, a plurality of groups of fixing columns are arranged in the groove, a working plate is arranged in the tool area, fixing ends are arranged at two ends of the working plate, and fixing holes matched with the fixing columns are formed in the fixing ends.
According to the invention, the fixing columns are arranged in the grooves in an array mode, the marks are arranged on one sides of the fixing columns, the workbench and the box body are arranged in a drawer mode, and the first handle is arranged on one side of the workbench.
In the invention, sanitary tweezers, an adhesive tape and a water absorption film are arranged in a tool area, an acid cellulose film is arranged in an acid cellulose film area, a colloidal gold pad is arranged in a colloidal gold pad area, a sample film is arranged in a sample film area, and a backing plate is arranged on one side of the adhesive tape.
In the invention, one end of the temperature control box is arranged on the back of the box body, the handle II is arranged outside the temperature control box, and the temperature controller is arranged in the temperature control box.
According to the invention, a first partition plate which is matched with the second partition plate is arranged in the cover body, the height of the first partition plate is the same as the depth of the inner side of the cover body, a sterilizing lamp is arranged in the cover body above the material area, a power supply assembly is arranged on the outer side of the cover body, and the sterilizing lamp is electrically connected with the power supply assembly.
According to the invention, an opening is formed in one side of the box body, a magnetic stripe is arranged in one side of the opening in the box body, a lower cover body matched with the opening is arranged on the cover body, and a handle is arranged on one side of the cover body.
The invention provides an application method of a tuberculosis detection kit, which specifically comprises the following steps:
(1) collecting 5ml of venous blood of a tester, centrifuging at 1500-3000 r/min for 5-15 min, and taking upper serum as a serum sample;
(2) pulling out the workbench from one side of the detection kit through a first handle, opening the cover body through the first handle, taking out the sanitary tweezers, the working plate and the adhesive tape from the tool area, clamping the working plate by using the sanitary tweezers, aligning the fixing holes with the fixing columns, putting the working plate in, placing the adhesive tape on the upper surface of the working plate by using the sanitary tweezers, and flattening;
(3) sampling according to the upward sequence of the step-shaped partition plates, firstly, taking out the water absorption film from the tool area by using a sanitary forceps and placing the water absorption film at the lower end of the backing plate, secondly, taking out the acid cellulose film from the acid cellulose film area, placing the bottom end of the acid cellulose film below the upper end of the water absorption film, then, taking out the colloidal gold pad from the colloidal gold pad area, placing the bottom end of the colloidal gold pad above the upper end of the acid cellulose film, and finally, taking out the sample film from the sample film area, and placing the bottom end of the sample film above the upper end;
(4) respectively fixing an anti-gold-labeled tubercle bacillus antibody in an anti-gold-labeled antibody bottle and a tubercle bacillus antibody in the antibody bottle on a C line and a T line of an acid cellulose membrane, taking a sealing liquid bottle and a washing liquid bottle, respectively dripping 1ml of sealing liquid and washing liquid on one side of the C line, between the C line and the T line and on one side of the T line on the acid cellulose membrane, dripping 60-80 mu l of serum sample on one end of a dried sample membrane, moving the serum sample to a water absorption membrane along the sample membrane due to capillary action and the inclined arrangement of a workbench, forming a gold-labeled serum sample through a colloidal gold pad, and observing whether red spots are visible by naked eyes on the C line and the T line when the gold-labeled serum sample moves to the acid cellulose membrane fixed with the tubercle bacillus antibody;
(5) after the detection is finished, the working plate is taken down from the fixed column by using the sanitary tweezers, the workbench is disinfected by using the disinfectant, and the workbench is placed back into the box body after disinfection.
Preferably, the cellulose acetate membrane is adopted as the acid cellulose membrane, the washing solution bottle is a PBS reagent bottle, and the blocking solution bottle is a 5% BSA blocking solution bottle.
By implementing the technical scheme of the invention, the following beneficial effects can be achieved:
the invention aims to provide a kit for detecting pulmonary tuberculosis and application thereof, the kit can be used for matching with a step-shaped partition plate to quickly finish the preparation work of colloidal gold method detection, has repeatable operability, can detect whether a tuberculosis antigen exists in a serum sample by using a double-antibody sandwich method, has high specificity, high sensitivity and high accuracy detection performance by matching with the inclined arrangement of a worktable, can quickly screen out a positive sample of the serum with the tuberculosis antigen, can quickly and conveniently assist in diagnosing the pulmonary tuberculosis, has the detection time of 3-5min, and has wide practicability and development value in the field of disease detection.
Drawings
Fig. 1 shows a schematic structural diagram of a tuberculosis detection kit provided by the invention.
Fig. 2 is a schematic view of a workbench according to the present invention.
Fig. 3 shows a rear view of the kit for detecting pulmonary tuberculosis provided by the invention.
Fig. 4 is a schematic view illustrating a use state of the work board according to the present invention.
In FIGS. 1-4, 1-box, 11-magnetic stripe, 12-workbench, 121-groove, 122-fixing column, 123-handle I, 124-mark, 125-working plate, 126-adhesive tape, 127-fixing end, 128-fixing hole, 13-clapboard II, 2-step clapboard, 21-tool area, 211-backing plate, 212-water absorbing film, 22-acid cellulose film area, 221-acid cellulose film, 222-C line, 223-T line, 23-colloidal gold pad area, 231-colloidal gold pad, 24-sample film area, 241-sample film, 3-cover, 31-germicidal lamp, 311-power supply assembly, 32-lower cover, 33-handle, 34-clapboard I, 4-reagent area, 41-closed liquid bottle, 42-anti-gold-labeled antibody bottle, 43-washing liquid bottle, 44-antibody bottle, 45-partition plate III, 451-temperature control box and 452-handle II.
Detailed Description
The present invention will be described below by way of examples with reference to FIGS. 1 to 4, but the present invention is not limited to the following examples.
In the present invention, for convenience of description, the description of the relative position relationship of each component in the kit for detecting pulmonary tuberculosis is described according to the layout mode of fig. 1, such as: the positional relationship of up, down, left, right, etc. is determined in accordance with the layout direction of fig. 1.
The magnetic strip 11, the adhesive tape 126, the backing plate 211, the water absorption film 212, the acid cellulose film 221, the colloidal gold pad 231, the sample film 241, the germicidal lamp 31, the power supply assembly 311, the sealing liquid, the anti-gold-labeled antibody, the washing liquid, the temperature control box 451 and the hygienic tweezers used in the present invention can be purchased or customized through public channels.
All materials, reagents and equipment selected for use in the present invention are well known in the art, but do not limit the practice of the invention, and other reagents and equipment well known in the art may be suitable for use in the practice of the following embodiments of the invention.
The first embodiment is as follows: pulmonary tuberculosis detection kit
As shown in fig. 1-4, a kit for detecting pulmonary tuberculosis includes a box body 1 and a cover body 3, the cover body 3 is disposed above the box body 1, the box body 1 is divided into a material region and a reagent region 4 by a partition plate two 13, a step-shaped partition plate 2 is disposed in the material region, a sample membrane region 24, a colloidal gold pad region 23, an acid cellulose membrane region 22 and a tool region 21 are sequentially disposed on the step-shaped partition plate 2 from top to bottom, a workbench 12 is disposed on the box body 1 below the step-shaped partition plate 2, the workbench 12 is disposed in an inclined manner, a partition plate three 45 is disposed in the reagent region 4, a closed liquid bottle 41, an anti-gold-labeled antibody bottle 42, a washing liquid bottle 43 and an antibody bottle 44 are disposed on the partition plate three 45.
In the invention, a groove 121 is arranged on the working table 12, a plurality of groups of fixing columns 122 are arranged in the groove 121, a working plate 125 is arranged in the tool area 21, fixing ends 127 are arranged at two ends of the working plate 125, and fixing holes 128 matched with the fixing columns 122 are arranged on the fixing ends 127.
In the invention, the fixing columns 122 are arranged in the groove 121 in an array manner, the marks 124 are arranged on one side of the fixing columns 122, the workbench 12 and the box body 1 are arranged in a drawer manner, and the first handle 123 is arranged on one side of the workbench 12.
In the invention, hygienic tweezers, an adhesive tape 126 and a water absorption film 212 are placed in a tool area 21, an acid cellulose film 221 is placed in an acid cellulose film area 22, a colloidal gold pad 231 is placed in a colloidal gold pad area 23, a sample film 241 is placed in a sample film area 24, and a backing plate 211 is arranged on one side of the adhesive tape 126.
In the invention, one end of the temperature control box 451 is arranged on the back surface of the box body 1, the second handle 452 is arranged outside the temperature control box 451, and the temperature controller is arranged in the temperature control box 451.
In the invention, the first partition plate 34 matched with the second partition plate 13 is arranged in the cover body 3, the height of the first partition plate 34 is the same as the depth of the inner side of the cover body 3, the sterilizing lamp 31 is arranged in the cover body 3 above the material area, the power supply assembly 311 is arranged outside the cover body 3, the reagent area 4 is not influenced when the material area is sterilized, and the sterilizing lamp 31 is electrically connected with the power supply assembly 311.
In the invention, an opening is arranged on one side of a box body 1, a magnetic strip 11 is arranged on one side of the opening on the box body 1, a lower cover body 32 matched with the opening is arranged on a cover body 3, and a handle 33 is arranged on one side of the cover body 3.
Example two: application of pulmonary tuberculosis detection kit
An application method of a tuberculosis detection kit specifically comprises the following steps:
(1) collecting 5ml of venous blood of a tester, centrifuging at 1500-3000 r/min for 5-15 min, and taking upper serum as a serum sample;
(2) pulling out the workbench 12 from one side of the detection kit through a first handle 123, opening the cover body 3 through a handle 33, taking out the sanitary forceps, the working plate 125 and the adhesive tape 126 from the tool area 21, clamping the working plate 125 by using the sanitary forceps, aligning the fixing hole 128 with the fixing column 122, putting the working plate 125 in, placing the adhesive tape 126 on the upper surface of the working plate 125 by using the sanitary forceps, and flattening;
(3) sampling in the order of the step-like partition 2 upward, first, the water-absorbent membrane 212 is taken out from the tool region 21 and placed at the lower end of the backing plate 211 using hygienic forceps, second, the cellulose acylate membrane 221 is taken out from the cellulose acylate membrane region 22, the bottom end of the cellulose acylate membrane 221 is placed below the upper end of the water-absorbent membrane 212, then, the gold colloidal pad 231 is taken out from the gold colloidal pad region 23, the bottom end of the gold colloidal pad 231 is placed above the upper end of the cellulose acylate membrane 221, and finally, the sample membrane 241 is taken out from the sample membrane region 24, the bottom end of the sample membrane 241 is placed above the upper end of the gold;
(4) respectively fixing the anti-gold-labeled tubercle bacillus antibody in the anti-gold-labeled antibody bottle 42 and the tubercle bacillus antibody in the antibody bottle 44 on a C line 222 and a T line 223 of an acid cellulose membrane 221, taking a sealing liquid bottle 41 and a washing liquid bottle 43, dripping 1ml of sealing liquid and washing liquid on one side of the C line 222, between the C line 222 and the T line 223 and on one side of the T line 223 on the acid cellulose membrane 221 respectively, dripping 60-80 microliter of serum sample on one end of a dried sample membrane 241, moving the serum sample to a water absorption membrane 212 along the sample membrane 241 due to capillary action and the inclined arrangement of a workbench 12, forming a gold-labeled serum sample through a colloidal gold pad 231, and observing whether a spot which can be seen by naked eyes on the C line 222 and the T line 223 or not when the gold-labeled serum sample moves to the acid cellulose membrane 221 fixed with the tubercle bacillus antibody;
(5) after the detection is finished, the working plate 125 is taken down from the fixing column 122 by using sanitary tweezers, the working table 12 is sterilized by using a disinfectant, and the working table 12 is put back into the box body 1 after the disinfection.
Preferably, the cellulose acetate membrane 221 is cellulose acetate membrane, the washing solution bottle 43 is selected from a PBS reagent bottle, and the blocking solution bottle 41 is selected from a 5% BSA blocking solution bottle 41.
Example three: effect experiment of pulmonary tuberculosis detection kit
The study subjects are 150 PTB patients treated in 6 months-2018 months in 2017, all the patients are accompanied by cough and expectoration symptoms, the disease course time exceeds 14 days, and the patients are confirmed to be tuberculosis patients by combining the clinical diagnosis and treatment guide-tuberculosis volume compiled by the Chinese medical society; 30 patients with other respiratory diseases, not tuberculosis, who are treated at the same time are selected; patients with immune system diseases are excluded from the two groups of patients, and patients taking immunosuppressant medicines in the near term are excluded; and all signed informed consent was willing to participate in the study.
3.1 diagnostic sensitivity
100 serum samples of PTB confirmed patients were collected from the clinic and tested using the kit for tuberculosis detection of example one according to the procedure of the application method of example two. If the C line and the T line both have visible red spots, the judgment result is positive. After 3-5min, the statistical results are as follows:
TABLE 1 diagnostic sensitivity test of tuberculosis detection kit
According to the statistical results in table 1, the number of positive samples with tuberculosis antigens was 93 and the number of negative samples was 7 in the detection of 100 sera of diagnosed PTB patients. Therefore, the diagnostic sensitivity (%) was 93/100-93% by calculation.
3.2 diagnostic specificity
Serum from 200 healthy blood donors was collected from the clinic, and the serum collected from the healthy blood donors was examined using the kit for detecting pulmonary tuberculosis according to the procedure of the application method of example two. If the C line and the T line both have visible red spots, the judgment result is positive. After 3-5min, the statistical results are as follows:
TABLE 2 diagnostic specificity test of tuberculosis detection kit
According to the statistical results in table 2, the number of positive samples with tuberculosis antigens was found to be 2 and the number of negative samples was found to be 198 in the detection of 200 sera from healthy donors. Therefore, the diagnostic sensitivity (%) of 198/200% to 99% was calculated.
3.3 comparative test
150 PTB patients and 30 non-tuberculosis respiratory disease patients treated at the same time are respectively subjected to the detection of the tuberculosis detection kit provided by the invention (the method of the second embodiment, if red spots are visible on C lines and T lines, the detection is judged to be positive), sputum smear detection (oral sputum is collected from a detected person, a smear mirror is used for Ziehl-Neelsen staining examination, bacteria culture is carried out by using an acidic Roche solid medium, positive strains are identified by using a p-nitrobenzoic acid (PNB) method, a positive judgment standard is that the number of tubercle bacillus in each 100 visual fields is positive when 3-9 are detected), TB-SA detection (4 ml of venous blood of all the included persons is collected, upper layer serum is taken after centrifugal treatment (3000r/min) for 10min, TB-SA antibody detection is carried out by using the kit provided by Saipack company, the specific operation is carried out according to the specification, wherein (the OD value of the sample)/(the OD value of the critical reference substance) is more than or equal to 1.2, namely positive).
The coincidence rate is (number of positive cases in diagnosis + number of negative cases in diagnosis)/total number of people in diagnosis x 100%; the sensitivity is the number of positive cases/the number of patients confirmed to be positive x 100%; the specificity is the number of negative cases/negative cases x 100%. The statistical results of this experiment are shown in Table 3.
TABLE 3 comparative testing of tuberculosis detection kits
As can be seen from Table 3, the sensitivity and the diagnosis coincidence rate of the kit for detecting pulmonary tuberculosis provided by the invention are higher than those of the other two groups, the data difference has statistical significance (P is less than 0.05), and the specificity data difference of the 3 groups of methods has no statistical significance (P is more than 0.05).
In conclusion, the kit for detecting the pulmonary tuberculosis and the application thereof provided by the invention can be used for matching with the step-shaped partition plate 2 to quickly finish the preparation work of the colloidal gold method detection, has repeatable operability, can be used for detecting whether a tuberculosis antigen exists in a serum sample by using a double-antibody sandwich method, has high-specificity, high-sensitivity and high-accuracy detection performance by matching with the inclined arrangement of the worktable 12, can quickly screen out a positive sample of the serum with the tuberculosis antigen, can quickly and conveniently assist in diagnosing the pulmonary tuberculosis, has the detection time of 3-5min, and has wide practicability and development value in the field of disease detection.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (4)
1. A pulmonary tuberculosis detection kit comprises a kit body and a cover body, and is characterized in that the cover body is arranged above the kit body, the interior of the kit body is divided into a material area and a reagent area by a partition plate, a step-shaped partition plate is arranged in the material area, a sample membrane area, a colloidal gold pad area, an acid cellulose membrane area and a tool area are sequentially arranged on the step-shaped partition plate from top to bottom, a workbench is arranged on the kit body below the step-shaped partition plate, the workbench is obliquely arranged, a partition plate III is arranged in the reagent area, a sealing liquid bottle, an anti-gold-labeled antibody bottle, a washing liquid bottle and an antibody bottle are arranged on the partition plate III; a groove is formed in the workbench, a plurality of groups of fixing columns are arranged in the groove, a working plate is arranged in the tool area, fixing ends are arranged at two ends of the working plate, and fixing holes matched with the fixing columns are formed in the fixing ends; the fixing columns are arranged in the grooves in an array mode, marks are arranged on one sides of the fixing columns, the workbench and the box body are arranged in a drawer mode, and a first handle is arranged on one side of the workbench; sanitary tweezers, an adhesive tape and a water absorption film are arranged in the tool area, an acid cellulose film is arranged in the acid cellulose film area, a colloidal gold pad is arranged in the colloidal gold pad area, a sample film is arranged in the sample film area, and a backing plate is arranged on one side of the adhesive tape; one end of the temperature control box is arranged on the back face of the box body, a handle II is arranged outside the temperature control box, and a temperature controller is arranged in the temperature control box.
2. The tuberculosis detection kit of claim 1, wherein a first partition plate adapted to the first partition plate is arranged in the cover, the height of the first partition plate is the same as the depth of the inner side of the cover, a germicidal lamp is arranged in the cover above the material region, a power supply assembly is arranged on the outer side of the cover, and the germicidal lamp is electrically connected with the power supply assembly.
3. The tuberculosis detection kit of claim 1, wherein an opening is formed in one side of the box body, a magnetic stripe is arranged on one side of the opening in the box body, a lower cover body matched with the opening is arranged on the cover body, and a handle is arranged on one side of the cover body.
4. The application method of the tuberculosis detection kit according to claim 1, which is characterized by comprising the following steps:
(1) collecting 5ml of venous blood of a tester, centrifuging at 1500-3000 r/min for 5-15 min, and taking upper serum as a serum sample;
(2) pulling out the workbench from one side of the detection kit through a first handle, opening the cover body through the first handle, taking out the sanitary tweezers, the working plate and the adhesive tape from the tool area, clamping the working plate by using the sanitary tweezers, aligning the fixing holes with the fixing columns, putting the working plate in, placing the adhesive tape on the upper surface of the working plate by using the sanitary tweezers, and flattening;
(3) sampling according to the upward sequence of the step-shaped partition plates, firstly, taking out the water absorption film from the tool area by using a sanitary forceps and placing the water absorption film at the lower end of the backing plate, secondly, taking out the acid cellulose film from the acid cellulose film area, placing the bottom end of the acid cellulose film below the upper end of the water absorption film, then, taking out the colloidal gold pad from the colloidal gold pad area, placing the bottom end of the colloidal gold pad above the upper end of the acid cellulose film, and finally, taking out the sample film from the sample film area, and placing the bottom end of the sample film above the upper end;
(4) respectively fixing an anti-gold-labeled tubercle bacillus antibody in an anti-gold-labeled antibody bottle and a tubercle bacillus antibody in the antibody bottle on a C line and a T line of an acid cellulose membrane, taking a sealing liquid bottle and a washing liquid bottle, respectively dripping 1ml of sealing liquid and washing liquid on one side of the C line, between the C line and the T line and on one side of the T line on the acid cellulose membrane, dripping 60-80 mu l of serum sample on one end of a dried sample membrane, moving the serum sample to a water absorption membrane along the sample membrane due to capillary action and the inclined arrangement of a workbench, forming a gold-labeled serum sample through a colloidal gold pad, and observing whether red spots are visible by naked eyes on the C line and the T line when the gold-labeled serum sample moves to the acid cellulose membrane fixed with the tubercle bacillus antibody;
(5) after the detection is finished, the working plate is taken down from the fixed column by using sanitary tweezers, the workbench is disinfected by using disinfectant, and the workbench is placed back into the box body after disinfection;
the acid cellulose membrane adopts a cellulose acetate membrane, the washing liquid bottle is a PBS reagent bottle, and the sealing liquid bottle is a 5% BSA sealing liquid bottle.
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