CN109906842B - Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing - Google Patents
Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing Download PDFInfo
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 66
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 66
- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 36
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 36
- 241001478315 Candidatus Liberibacter asiaticus Species 0.000 title claims abstract description 11
- 206010020751 Hypersensitivity Diseases 0.000 title abstract description 7
- 229940088598 enzyme Drugs 0.000 claims abstract description 65
- 239000004365 Protease Substances 0.000 claims abstract description 31
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 23
- 239000008103 glucose Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 20
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 20
- 239000010457 zeolite Substances 0.000 claims abstract description 20
- 239000004382 Amylase Substances 0.000 claims abstract description 15
- 102000013142 Amylases Human genes 0.000 claims abstract description 15
- 108010065511 Amylases Proteins 0.000 claims abstract description 15
- 108010004032 Bromelains Proteins 0.000 claims abstract description 15
- 108010059892 Cellulase Proteins 0.000 claims abstract description 15
- 102000004882 Lipase Human genes 0.000 claims abstract description 15
- 108090001060 Lipase Proteins 0.000 claims abstract description 15
- 239000004367 Lipase Substances 0.000 claims abstract description 15
- 108090000526 Papain Proteins 0.000 claims abstract description 15
- 102000019197 Superoxide Dismutase Human genes 0.000 claims abstract description 15
- 108010012715 Superoxide dismutase Proteins 0.000 claims abstract description 15
- 235000019418 amylase Nutrition 0.000 claims abstract description 15
- 235000019835 bromelain Nutrition 0.000 claims abstract description 15
- 229940106157 cellulase Drugs 0.000 claims abstract description 15
- 235000019421 lipase Nutrition 0.000 claims abstract description 15
- 229940055729 papain Drugs 0.000 claims abstract description 15
- 235000019834 papain Nutrition 0.000 claims abstract description 15
- 150000001875 compounds Chemical class 0.000 claims abstract description 14
- 238000010564 aerobic fermentation Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 230000035800 maturation Effects 0.000 claims description 2
- 241000207199 Citrus Species 0.000 abstract description 9
- 235000020971 citrus fruits Nutrition 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 230000002210 biocatalytic effect Effects 0.000 abstract description 3
- 238000000354 decomposition reaction Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 229940079919 digestives enzyme preparation Drugs 0.000 abstract description 2
- 235000013399 edible fruits Nutrition 0.000 description 7
- 239000002689 soil Substances 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000008859 change Effects 0.000 description 3
- -1 oligosaccharidase Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 241000534456 Arenaria <Aves> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 241001466030 Psylloidea Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 241001233061 earthworms Species 0.000 description 1
- ZINJLDJMHCUBIP-UHFFFAOYSA-N ethametsulfuron-methyl Chemical compound CCOC1=NC(NC)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)C(=O)OC)=N1 ZINJLDJMHCUBIP-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000005078 fruit development Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0154—Litter comprising inorganic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
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Abstract
The present application is a divisional application of 201811529411.4. The invention discloses an application of a hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing, belonging to the technical field of enzyme preparations. The protein complex enzyme preparation is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain. The protein complex enzyme preparation has strong biocatalytic decomposition effect, also has the effect of preventing and treating the huanglongbing, can solve the problem that the citrus is very easy to have the huanglongbing in the planting process, and is suitable for preventing and treating the citrus huanglongbing.
Description
The application is a divisional application with the application number of 201811529411.4, application date of 2018, 12 months and 13 days, and the invention name of 'a hypersensitive protein complex enzyme preparation and a preparation method and application thereof'.
Technical Field
The invention relates to an application of a hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing, belonging to the technical field of enzyme preparations.
Background
The citrus greening disease is very easy to appear in the planting process of the citrus, and loss is brought to farmers, so that the prevention and control of the citrus greening disease are of great significance to citrus planting.
Disclosure of Invention
In order to solve the problem that citrus is easy to have yellow shoot, the invention provides the application of the hypersensitive protein complex enzyme preparation in preventing and treating the yellow shoot, and the adopted technical scheme is as follows:
the invention provides an application of a protein complex enzyme preparation in preventing and treating huanglongbing, in particular to an application in preventing and treating citrus huanglongbing, wherein the protein complex enzyme preparation is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain.
Preferably, the complex enzyme, the zeolite powder, the glucose and the water are mixed according to the following parts by weight: 5.72 to 8.82 parts by weight of complex enzyme; 3.8 to 5.7 parts by weight of zeolite powder; 0.39 to 1.14 parts by weight of glucose; 7.6 to 11.4 parts by weight of water; wherein: the 5.72-8.82 parts by weight of complex enzyme is composed of 0.1-0.15 part by weight of superoxide dismutase, 1.5-2.5 parts by weight of bromelain, 2-3 parts by weight of cellulase, 1-1.5 parts by weight of lipase, 1-1.5 parts by weight of amylase, 0.05-0.07 part by weight of oligosaccharidase and 0.07-0.1 part by weight of papain.
More preferably, the complex enzyme, the zeolite powder, the glucose and the water are mixed according to the following parts by weight: 7.26 parts of compound enzyme; 4.2 parts of zeolite powder; 0.78 weight part of glucose; 9.4 parts by weight of water; wherein: the 7.26 parts by weight of complex enzyme consists of 0.12 part by weight of superoxide dismutase, 2 parts by weight of bromelain, 2.5 parts by weight of cellulase, 1.3 parts by weight of lipase, 1.2 parts by weight of amylase, 0.06 part by weight of oligosaccharidase and 0.08 part by weight of papain.
Preferably, the preparation method of the protein complex enzyme preparation comprises the steps of mixing the complex enzyme, the zeolite powder and water, adding oxygen, stirring uniformly, carrying out aerobic fermentation for 10-12 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adjusting the pH value to 6.5-6.8, adding glucose, continuing aerobic fermentation, then carrying out post-maturation culture for 45-50 days, standing after the culture is finished, and filtering to obtain the protein complex enzyme preparation.
More preferably, the number of days of aerobic fermentation is 10 days.
More preferably, the post maturation incubation is stirred every 15h during fermentation for 30min each time.
More preferably, the time of standing is 45 days.
The invention relates to a hypersensitive protein complex enzyme preparation which exists in a protein form and is not a microbial inoculum, the protein complex enzyme preparation has a strong biocatalytic decomposition effect, the negative reaction of harmful microorganisms in organisms is catalyzed and inhibited by catalyzing the positive reaction of beneficial microorganisms in the organisms, the balance of microecology is realized, the cell activity can be excited, heterologous proteins are rejected, the immunity of the organisms is greatly enhanced, the protein complex enzyme preparation has strong stability and strong anti-interference capability against foreign bacteria, is not damaged by foreign species, and has the characteristics of biological diversity and high catalytic reaction speed.
The invention has the beneficial effects that:
the invention provides a protein complex enzyme preparation which has strong biocatalytic decomposition effect. The protein complex enzyme preparation also has the effect of preventing and treating the huanglongbing.
Detailed Description
The present invention will be further described with reference to the following specific examples, but the present invention is not limited to these examples.
Example 1:
the embodiment provides a protein complex enzyme preparation, which is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; wherein:
5.72 parts of complex enzyme; 3.8 parts by weight of zeolite powder; 0.39 parts by weight of glucose; 7.6 parts by weight of water;
5.72 parts by weight of complex enzyme consists of 0.1 part by weight of superoxide dismutase, 1.5 parts by weight of bromelain, 2 parts by weight of cellulase, 1 part by weight of lipase, 1 part by weight of amylase, 0.05 part by weight of oligosaccharidase and 0.07 part by weight of papain.
The embodiment also provides a preparation method of the protein complex enzyme preparation, which comprises the steps of mixing superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose and water, then adding oxygen and stirring uniformly, carrying out aerobic fermentation for 10 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adding glucose to continue carrying out aerobic fermentation for 45 days, then adjusting the pH value to 6.5-6.8 to carry out post-maturation stage cultivation, stirring once every 15 hours during the fermentation period, stirring for 30min every time, standing for 45 days after the post-maturation stage cultivation is finished, and filtering to obtain the protein complex enzyme preparation.
Example 2
The embodiment provides a protein complex enzyme preparation, which is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; wherein:
8.82 parts of complex enzyme; 5.7 parts by weight of zeolite powder; 1.14 parts by weight of glucose; 11.4 parts by weight of water;
8.82 parts by weight of complex enzyme consists of 0.15 part by weight of superoxide dismutase, 2.5 parts by weight of bromelain, 3 parts by weight of cellulase, 1.5 parts by weight of lipase, 1.5 parts by weight of amylase, 0.07 part by weight of oligosaccharidase and 0.1 part by weight of papain.
The embodiment also provides a preparation method of the protein complex enzyme preparation, which comprises the steps of mixing superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose and water, then adding oxygen and stirring uniformly, carrying out aerobic fermentation for 10 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adding glucose to continue carrying out aerobic fermentation for 45 days, then adjusting the pH value to 6.5-6.8 to carry out post-maturation stage cultivation, stirring once every 15 hours during the fermentation period, stirring for 30min every time, standing for 45 days after the post-maturation stage cultivation is finished, and filtering to obtain the protein complex enzyme preparation.
Example 3
The embodiment provides a protein complex enzyme preparation, which is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; wherein:
7.26 parts of compound enzyme; 4.2 parts of zeolite powder; 0.78 weight part of glucose; 9.4 parts by weight of water;
7.26 parts by weight of complex enzyme consists of 0.12 part by weight of superoxide dismutase, 2 parts by weight of bromelain, 2.5 parts by weight of cellulase, 1.3 parts by weight of lipase, 1.2 parts by weight of amylase, 0.06 part by weight of oligosaccharidase and 0.08 part by weight of papain.
The embodiment also provides a preparation method of the protein complex enzyme preparation, which comprises the steps of mixing superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose and water, then adding oxygen and stirring uniformly, carrying out aerobic fermentation for 10 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adding glucose to continue carrying out aerobic fermentation for 45 days, then adjusting the pH value to 6.5-6.8 to carry out post-maturation stage cultivation, stirring once every 15 hours during the fermentation period, stirring for 30min every time, standing for 45 days after the post-maturation stage cultivation is finished, and filtering to obtain the protein complex enzyme preparation.
In order to verify the prevention and treatment effect of the protein complex enzyme on the salsa orange yellow dragon disease, experiments are carried out in apricot blossom town and Jiangkou town of Guangdong province, Mingkong county. Mixing the protein complex enzyme preparation in example 3 with water according to a mass ratio of 1:300 to prepare a protein complex enzyme solution, carrying out root cultivation on the citrus arenaria by using the protein complex enzyme solution prepared in example 3, observing the condition of hairy roots, and observing the condition of increasing organic matters for soil improvement. After the compound protease solution is sprayed on the leaf surfaces, the change of the survival rate of the psyllids is detected, the growth change and the tip control effect of the spring tips are observed, the change of channels and leaves is observed, the flowering quantity and the fruit setting rate are measured, and the detection, observation and evaluation work such as the condition that the branch leaves and the fruit development are normal is observed is carried out. The experimental acre is 9 mu.
And (3) test results: after the soil is improved, the organic matters of the soil are increased, the soil is soft, the air permeability is increased, harmful viruses are eliminated, the soil is recovered to be alive after three months, and a plurality of earthworms appear in the soil. The main root recovers the vitality and a large amount of hairy roots are visible. The branches and leaves grow, the leaves grow bigger and turn green, and the fruit setting rate is greatly improved. After the compound enzyme is used for one year, the fruit setting rate is improved, the fruit is full in autumn, the water content is sufficient, the sugar content is increased, and no yellow dragon disease virus is found after detection. The compound enzyme obtains favorable performance in the experimental process of treating the salose orange yellow dragon disease, and plants have clustered roots, luxuriant branches and leaves, quick recovery of fruit setting rate, full fruits, higher sugar content and better taste under the action of the compound enzyme and are praised by local fruit growers.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (2)
1. The application of the protein complex enzyme preparation in preventing and treating the huanglongbing is characterized in that the protein complex enzyme preparation is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; the compound enzyme, the zeolite powder, the glucose and the water are mixed according to the following weight parts: 7.26 parts of compound enzyme; 4.2 parts of zeolite powder; 0.78 weight part of glucose; 9.4 parts by weight of water; wherein: the 7.26 parts by weight of complex enzyme consists of 0.12 part by weight of superoxide dismutase, 2 parts by weight of bromelain, 2.5 parts by weight of cellulase, 1.3 parts by weight of lipase, 1.2 parts by weight of amylase, 0.06 part by weight of oligosaccharidase and 0.08 part by weight of papain; the preparation method of the protein complex enzyme preparation comprises the steps of mixing the complex enzyme, zeolite powder and water, adding oxygen, stirring uniformly, carrying out aerobic fermentation for 10-12 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, adding glucose, continuing the aerobic fermentation, then carrying out post-maturation stage cultivation for 45-50 days, then adjusting the pH value to 6.5-6.8, standing after the cultivation is finished, and filtering to obtain the protein complex enzyme preparation; the post maturation stage cultivation is stirred once every 15h during fermentation, and each stirring is carried out for 30 min; the standing time was 45 days.
2. Use according to claim 1, wherein the number of days of aerobic fermentation is 10 days.
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| CN201811529411.4A CN109618944B (en) | 2018-12-13 | 2018-12-13 | Hypersensitive protein complex enzyme preparation as well as preparation method and application thereof |
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| CN109906842B (en) * | 2018-12-13 | 2022-03-11 | 于凯波 | Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing |
| CN110064047A (en) * | 2019-04-23 | 2019-07-30 | 深圳前海千水生物科技有限公司 | A kind of plant complex enzyme nasal cavity detoxicating hydro-peening agent |
| CN110051828A (en) * | 2019-04-23 | 2019-07-26 | 深圳前海千水生物科技有限公司 | A kind of plant complex enzyme gynaecology detoxicating lotion |
| CN110074139A (en) * | 2019-05-13 | 2019-08-02 | 河南地卫士生物科技有限公司 | A kind of biological pesticide that Trichoderma compounds with super quick albumen complex enzyme |
| CN110742540A (en) * | 2019-10-15 | 2020-02-04 | 于凯波 | Rural closestool device based on compound enzyme decomposition |
| CN110800761B (en) * | 2019-11-01 | 2021-06-01 | 潍坊科技学院 | Signal protein-containing composition for preventing and treating root-knot nematode as well as preparation method and application thereof |
| CN111018580A (en) * | 2019-11-27 | 2020-04-17 | 于凯波 | Complex enzyme preparation capable of degrading animal wastes and preparation method and application thereof |
| CN111411100A (en) * | 2020-03-27 | 2020-07-14 | 于凯波 | Complex enzyme preparation capable of degrading kitchen waste and preparation method and application thereof |
| CN113402312A (en) * | 2021-06-23 | 2021-09-17 | 兰州大学 | Nano composite catalyst, device and method for treating agricultural wastes |
| CN115282264A (en) * | 2022-08-31 | 2022-11-04 | 北京志宣生物科技有限公司 | Compound preparation for reducing blood sugar and improving immunity and preparation method thereof |
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| CN102326588A (en) * | 2011-06-28 | 2012-01-25 | 蔡明� | Plant growth nutrient solution |
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| CN109906842A (en) | 2019-06-21 |
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