CN109749927A - A kind of detection device and detection method - Google Patents
A kind of detection device and detection method Download PDFInfo
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- CN109749927A CN109749927A CN201711081192.3A CN201711081192A CN109749927A CN 109749927 A CN109749927 A CN 109749927A CN 201711081192 A CN201711081192 A CN 201711081192A CN 109749927 A CN109749927 A CN 109749927A
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Abstract
The invention discloses a kind of detection device and detection method, the detection device is used to detect the effect of cleaning piece removal microorganism, comprising: platform assembly, for being coated with the microorganism；Slide assemblies, for driving the cleaning piece to wipe the microorganism on the platform assembly under the effect of external force；Wherein, the slide assemblies include the contact surface contacted with the platform assembly, the cleaning piece is fixed on the contact surface, when external force is when the slide assemblies, the slide assemblies drive the cleaning piece movement, to which analog subscriber wipes the platform assembly using the cleaning piece, to detect the effect that the cleaning piece removes the microorganism.By above-mentioned detection device, it is able to detect the effect of cleaning piece removal microorganism.
Description
Technical field
The present invention relates to detection technique fields, more particularly to a kind of detection device and detection method.
Background technique
With the development and progress of society, people increasingly pay attention to microorganism, such as the removing of bacterium.
Currently, people generally use cleaning piece, such as non-woven fabrics, wet tissue, paper handkerchief, rag to be purged microorganism.
Present inventor has found in long-term research, is able to detect cleaning piece currently without detection device and is using
Whether there are the effect of removal microorganism, or the quality of removal microorganism effect in the process, therefore leads to not wipe cleaning piece
The ability of microorganism makes assessment.
Summary of the invention
The invention mainly solves the technical problem of providing a kind of detection device and detection methods, are able to detect cleaning piece and go
Except the effect of microorganism.
In order to solve the above technical problems, one technical scheme adopted by the invention is that: a kind of detection device is provided, for examining
Survey the effect of cleaning piece removal microorganism, comprising:
Platform assembly, for being coated with the microorganism of predetermined amount；
Slide assemblies, for driving the cleaning piece to wipe micro- life on the platform assembly under the effect of external force
Object；
Wherein, the slide assemblies include the contact surface contacted with the platform assembly, and the cleaning piece is fixed on described
On contact surface, when external force is when the slide assemblies, the slide assemblies drive the cleaning piece movement, to simulate use
Family wipes the platform assembly using the cleaning piece, to detect the effect that the cleaning piece removes the microorganism.
In order to solve the above technical problems, another technical solution used in the present invention is: providing a kind of detection method, use
The effect of detection device detection cleaning piece removal microorganism described above, which comprises
The microorganism of predetermined amount is coated on the platform assembly；
The cleaning piece is fixed on the contact surface of the slide assemblies；
The mobile slide assemblies, so that the slide assemblies drive the cleaning piece to wipe the institute on the platform assembly
State microorganism；
The quantity of the remaining microorganism on the platform assembly is detected, to detect described in the cleaning piece removal
The effect of microorganism.
The beneficial effects of the present invention are: being in contrast to the prior art, detection device of the present invention is for detecting cleaning piece
Remove the effect of microorganism, comprising: platform assembly, for being coated with microorganism；Slide assemblies, for driving under the effect of external force
Cleaning piece wipes the microorganism on platform assembly；Wherein, slide assemblies include the contact surface contacted with platform assembly, and cleaning piece is solid
Fixed when external force is when slide assemblies, slide assemblies drive cleaning piece movement on the contact surface, wipe so that analog subscriber uses
Object wiping platform assembly is wiped, cleaning piece is able to detect by the detection device to detect the effect of cleaning piece removal microorganism and goes
Except the effect of microorganism.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.Wherein:
Fig. 1 is the structural schematic diagram of one embodiment of detection device of the present invention；
Fig. 2 is the positive structure schematic of another embodiment of detection device of the present invention；
Fig. 3 is the overlooking surface structural schematic diagram of detection device in Fig. 2 embodiment；
Fig. 4 is the side structure schematic diagram of detection device in Fig. 2 embodiment；
Fig. 5 is the schematic diagram of the section structure of detection device in Fig. 2 embodiment；
Fig. 6 is the enlarged diagram in Fig. 5 at A；
Fig. 7 is the enlarged diagram in Fig. 5 at B；
Fig. 8 be in Fig. 2 embodiment detection device in the structural schematic diagram of an application scenarios sliding block and briquetting；
Fig. 9 is the flow diagram of one embodiment of detection method；
Figure 10 is the flow diagram of another embodiment of detection method.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, rather than whole embodiments.Based on this
Embodiment in invention, those of ordinary skill in the art are obtained every other under the premise of not making creative labor
Embodiment shall fall within the protection scope of the present invention.
Refering to fig. 1, Fig. 1 is the structural schematic diagram of one embodiment of detection device of the present invention, the detection device in the present invention
For detecting the effect of cleaning piece removal microorganism, wherein cleaning piece can be non-woven fabrics, paper handkerchief, toilet paper, wet tissue etc., and
Cleaning piece can be sheet, be also possible to bulk, as long as the cleaning piece has a smooth wiping surface or can pass through
Apply pulling force etc. and form a smooth wiping surface, microorganism can be coliform, staphylococcus aureus, green pus bar
Bacterium, Salmonella enteritidis, Acinetobacter baumannii, Candida albicans etc., in the present embodiment, detection device include: platform group
Part 10 and slide assemblies 20.
Platform assembly 10 is used to be coated with the microorganism (not shown) of predetermined amount；Slide assemblies 20 are under the effect of external force
Cleaning piece 30 is driven to wipe the microorganism on platform assembly 10.
Specifically, slide assemblies 20 include the contact surface 201 contacted with platform assembly 10, cleaning piece 30 is fixed on contact surface
On 201, when external force is in slide assemblies 20, slide assemblies 20 drive cleaning piece 30 to move, so that analog subscriber uses wiping
It wipes object 30 and wipes platform assembly 10, to detect the effect that cleaning piece 30 removes microorganism.
That is, in the present embodiment, being simulated with platform assembly 20 to cleaning piece, the microorganism of predetermined amount being coated on platform
On component 20, the hand of people is simulated with slide assemblies 20, is wiped using cleaning piece 30 to cleaning piece to simulate people, is detected to wipe
The effect of the removal microorganism of object 30.
Refering to Fig. 2 to Fig. 5, wherein Fig. 2 is the positive structure schematic of another embodiment of detection device of the present invention, Fig. 3
It is the overlooking structure diagram of detection device in Fig. 2 embodiment, Fig. 4 is the side structure of detection device in Fig. 2 embodiment
Schematic diagram, Fig. 5 are the schematic diagram of the section structure of detection device in Fig. 2 embodiment.
In this embodiment, platform assembly 10 includes: guide rail 101 and detection plate 102.Slide assemblies 20 include: cunning
Block 202.
Guide rail 101 is U-shaped, including the first bottom plate 1011 and extended vertically upwards along 1011 two sides of the first bottom plate first
Side plate 1012, detection plate 102 are fixed on the first bottom plate 1011, and microorganism is coated in detection plate 102, are slided when external force is mobile
When component 20, slide assemblies 20 drive cleaning piece 30 to move in detection plate 102 along the direction that guide rail 101 limits, i.e., in this reality
It applies in mode, the surface of cleaning piece is waited for the surface simulation of detection plate 102.
Optionally, detection plate 102 is made of hard refractory resistant material, can be glass plate, ceramic wafer, by not
The composite plate etc. that the steel plate that becomes rusty is formed with ceramic wafer.
Optionally, in order to avoid since detection plate 102 is uneven or has depressed area, so as to cause after being coated with microorganism,
Microorganism is accommodated in depressed area, therefore in the present embodiment, and detection plate 102 is flat surface, for example, detection plate 102
Flatness is less than or equal to 1mm/50mm, or is less than or equal to 1mm/90mm.
Optionally, detection plate 102 generates inhibition, the table of detection plate 102 to the movement of slide assemblies 20 in order to prevent
Surface roughness is less than or equal to Ra6.4, if the surface of detection plate 102 is excessively smooth certainly, on the one hand can not effectively simulate wait wipe
The surface of object is wiped, is on the other hand easy to cause cleaning piece 30 that microorganism is pushed into detection plate 102 during wiping microorganism
One side, the microorganism on 102 surface of detection plate cannot be removed, therefore, the surface roughness of detection plate 102 is greater than Ra0.025,
Certainly, in other embodiments, the surface roughness of detection plate 102 can also be Ra0.1~Ra3.2, herein with no restrictions.
Optionally, detection plate 102 can be fixed on the first bottom plate 1011, for example, being fixed on the by glue etc.
On one bottom plate 1011, certainly, detection plate 102 can also be removably disposed in the first bottom plate by the first connection bolt 1021
On 1011, specifically, being provided with connecting hole (not shown) on the first bottom plate 1011, connecting screw hole (figure is provided in detection plate 102
Do not show), optionally, the connecting screw hole be blind hole, first connection bolt 1021 from below the first bottom plate 1011 pass through connecting hole with
Detection plate 102 connects, to prevent the first connection bolt 1021 from interfering to the movement of detection plate 102.
Optionally, be fixed on the first bottom plate 1011 to preferably will test plate 102, also for can facilitate installation and
Dismantle detection plate 102, in the present embodiment, platform assembly 10 further include: the first positioning plate 103 and the second positioning plate 104.
First positioning plate 103 and the second positioning plate 104 are fixed on the first bottom plate 1011, and are located at detection plate
102 along the front and rear sides of 20 direction of motion of slide assemblies, to clamp detection plate 102.When needing to take out detection plate 102, only
It needs will test plate 102 to take out directly between the first positioning plate 103 and the second positioning plate 104, at this point, detection plate 102
Without being fixedly installed or being detachably arranged on the first bottom plate 1011 by additional part.
Optionally, it will test plate 102 for convenience and taken out between the first positioning plate 103 and the second positioning plate 104, refering to
Fig. 6, Fig. 6 are the enlarged diagrams in Fig. 5 at A, and the junction of detection plate 102 and the first positioning plate 103 is equipped with notch section 1022,
One end close to the detection plate 102 of notch section 1022 is equipped with inclined plane part 1023, so that clamping device such as tweezers etc., are easy by lacking
Oral area 1022 and inclined plane part 1023 extend into 102 lower section of detection plate and clamp detection plate 102.
Optionally, avoid clamping device during clamping detection plate 102, detection plate 102 falls off from clamping device,
The measure for increasing frictional force is also taken close to the lower section of the detection plate 102 of notch section 1022, for example, can be close in detection plate 102
Groove, protrusion, decorative pattern etc. is arranged in the lower section of 1022 side of notch section.
Optionally, the first positioning plate 103 and the second positioning plate 104 are removably disposed on the first bottom plate 1011.Specifically
Ground, the first positioning plate 103 are fixed on the first bottom plate 1011 by the second connecting bolt 1031.Second positioning plate 104 passes through positioning
Bolt 1042 is fixed on the first bottom plate 1011, and the second positioning plate 104 is being equipped with positioning along the direction that slide assemblies 20 move
Second positioning plate 104 is fixed on the first bottom plate 1011 by hole 1041, positioning bolt 1042 by location hole 1041, location hole
1041 length is greater than the diameter of positioning bolt 1042, and when unclamping positioning bolt 1042, the second positioning plate 104 can be along guide rail
It is moved in the length range of location hole 1041 in 101 directions limited.
Refering to the enlarged diagram that Fig. 7, Fig. 7 are in Fig. 5 at B, sliding block 202 is placed in detection plate 102, passes through contact surface
201 contact with detection plate 102, and cleaning piece 30 is fixed on contact surface 201, and when the mobile sliding block 202 of external force, sliding block 202 is driven
Cleaning piece 30 moves in detection plate 102.
Optionally, the contact surface 201 of sliding block 202 is completely covered in cleaning piece 30, to simulate cleaning piece 30 in normal use mistake
Cheng Zhongyu waits for the contact situation on cleaning piece surface.Optionally, in order to which more preferable simulation the user's palm or finger are using wiping
Object 30 is with to the contact situation on cleaning piece surface, and in an application scenarios, the surface area of contact surface 201 is less than or equal to
3600mm2, in another application scene, the surface area of contact surface 201 is less than or equal to 400mm2, and it is greater than 25mm2。
Optionally, in order to which cleaning piece 30 to be completely covered on contact surface 301, in the present embodiment, cleaning piece 30 is logical
It crosses and coats the mode of entire sliding block 202 and be fixed on contact surface 201, and in order to which cleaning piece 30 is more easily fixed on contact surface
On 201, settable fixture (not shown) or adhesive (not shown) are for fixing cleaning piece on the contact surface 201 of sliding block 202
30。
When cleaning piece surface, certain pressure can applied to cleaning piece 30 using the wiping of cleaning piece 30 usually using person
The surface to cleaning piece is wiped afterwards, therefore in the present embodiment, by the gravity simulation user of sliding block 202 to cleaning piece 30
The quality settings of sliding block 202 can be 50g~500g in an application scenarios by the pressure of application, in another application scene,
It is 100g~300g by the quality settings of sliding block 202.Simultaneously pressure of the sliding block 202 to cleaning piece 30 can also be set to
0.3g/mm2~0.8g/mm2, or by pressure of the sliding block 202 to cleaning piece 30 set 0.45g/mm2~0.65g/mm2, with more
The use process of good simulation cleaning piece 30.
Optionally, in order to make sliding block 202 in the lesser situation of area when contact surface 301, there is suitable shape ruler
It is very little with convenient to use, in the present embodiment, sliding block 202 is T-shaped, including the first slide section 2021 and the second slide section
2022, the first slide section 2021 is located at the downside of the second slide section 2022, and the first slide section 2021 includes contact surface 201, and the
Volume of the volume of one slide section 2021 less than the second slide section 2022.Meanwhile in order to reduce the overall volume of sliding block 202, with
And reduce the factors such as temperature and the quality of sliding block 202 is had an impact, stainless steel can be used in sliding block 202 or lead prepared material is made.
Optionally, in an application scenarios of present embodiment, in order to the quality of neatly adjusting slider 202,
Slide block assembly 20 further include: briquetting 203.
It is the structural schematic diagram of sliding block and briquetting refering to Fig. 8, Fig. 8, the one end opposite with contact surface 201 of sliding block 202 is equipped with
Recessed poor 2023, briquetting 203 is accommodated in groove 2023, so as to replace different briquettings 203 with the quality of adjusting slider 202
And sliding block 202 is to the pressure of contact surface 201.Optionally, briquetting 203 is taken out out of groove 2023 for convenience, briquetting 203
Height be greater than the depth of groove 2023, and it is optional, for the convenience of the users to the crawl of briquetting 203, may be used also on briquetting 203
Handle portion (not shown) is arranged.Optionally, when cleaning piece 30 be coated on sliding block 202 it is outer when, briquetting 203 can also be pressed in wiping
On object 30, plays the role of that cleaning piece 30 is helped steadily to be coated on sliding block 202, cleaning piece 30 is prevented to be loosened.
Optionally, external force slides the generation active force of sliding block 202 to influence test result in vertical direction in order to prevent
Dynamic component 20 further include: frame 204.
Frame 204 is inverted u-shaped, and frame 204 includes the second bottom plate 2041 and extends vertically downward along the second bottom plate two sides
The second side plate 2042, the second bottom plate 2041 is equipped with through-hole 2043, and frame 204 is fastened on guide rail 101, and sliding block 202 accommodates
It in through-hole 2043, is contacted with detection plate 102, when external force mobile framework 204, frame 204 is with movable slider 202 along guide rail 101
The direction of restriction moves, at this point, when external force applies active force to frame 204 in the vertical direction, since sliding block 202 is accommodated in
In through-hole 2043, the active force of vertical direction will be unable to act on sliding block 202, to make sliding block 202 not by the work of vertical direction
Firmly.
Optionally, frame 204 plays extruding to the cleaning piece 30 being coated on sliding block 202 during the motion in order to prevent
Effect, in the present embodiment, along the direction that frame 204 moves, frame 204 is additionally provided with first baffle 2044 and second baffle
2045, first baffle 2044 is fixedly connected with the first end of second baffle 2045 with frame 204, first baffle 2044 and second gear
The second end of plate 2045 and first end are rectangular, when sliding block 202 is accommodated in through-hole 2043, first baffle 2044 and second gear
The second end of plate 2045 is contacted with sliding block 202.
Optionally, slide assemblies 20 further include: power mechanism (not shown), power mechanism are arranged on frame 204, are used for
Driver framework 204 is moved along the direction that guide rail limits, specifically, power mechanism can be cylinder, hydraulic cylinder, electromagnet, straight line
Motor or rotary electric machine etc..
Optionally, in order to limit motion range of the sliding block 202 on guide rail 101, detection device further include: limit assembly
40, limit assembly 40 includes the first limit plate 401 and the second limit plate 402, the first limit plate 401 and the second limit plate
402 is inverted u-shaped, and the first limit plate 401 and the second limit plate 402 are fastened on the first side plate 1012 of guide rail 101, works as sliding
When component 20 moves to the position of the first limit plate 401, the second limit plate 402, the first limit plate 401, the resistance of the second limit plate 402
Sliding stop component 20 continues to move, so that slide assemblies 20 are transported between the first limit plate 401 and the second limit plate 402
It is dynamic.
Certainly, in other embodiments, limit assembly 40 can also be other structures form, such as be arranged in the first side plate
1012 or first convex block, the bolt etc. that are protruded towards 202 direction of sliding block on bottom plate 1011, herein with no restrictions.
It is the flow diagram of one embodiment of detection method refering to Fig. 9, Fig. 9, the detection method is using above-mentioned
The effect of detection device detection cleaning piece removal microorganism in any one embodiment, specific detection device can be found in above-mentioned
Embodiment, details are not described herein, the detection method in the present invention is described in detail below with reference to Fig. 2 to Fig. 5, the inspection
Survey method includes:
S10: the microorganism of predetermined amount is coated on platform assembly.
Specifically, being coated with the microorganism of predetermined amount in the detection plate 102 of platform assembly 10, wherein microorganism can be
Coliform, staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enteritidis, Acinetobacter baumannii, Candida albicans etc..
Optionally, in an application scenarios, microorganism can also be formed simulating pollution object, to simulate to cleaning piece surface
The situation of larger pollution is received, which includes microorganism and contaminated liquid, which includes bovine serum albumin water
Solution, such as 5g/L~7g/L can be set by bovine serum albumin content in the bovine serum albumin aqueous solution.
Specifically, in the present embodiment, microorganism is configured to aqueous solution, microbial solution is stirred by such as test tube
It mixes device to be stirred uniformly, is uniformly distributed bacterium solution, a certain amount of, such as the microorganism of 0.01ml, 0.1ml is then taken with liquid-transfering gun
Solution, in the central streak inoculation of detection plate 102, with platinum oese or directly with liquid-transfering gun pipette tips along 102 central area of detection plate
Domain is smeared and is opened, and microorganism coating is formed.
Optionally, in order to which microorganism to be fixed in detection plate 102, microbial solution is being coated on detection plate 102, also
Include the steps that dry detection plate 102, wherein can be the drying by way of gravity-flow ventilation, set for example, will test plate 102
It is dried in positions such as desinfection chamber, aseptic working platforms.
Optionally, it wipes for convenience and simulation wipes the situation for using wetted wipe 30 in practical wiping process,
After drying steps, further include the steps that the surface to dry detection plate 102 soaks, such as in the table of detection plate 102
Face cloth spills a certain amount of water or dilution, the sodium chloride solution (physiological saline) which is 0.9%.
Wherein, predetermined amount can be by being calculated, specifically, can refer to before being coated with microbial solution
The method of " measurement of GB4789.2-2016 food microbiological examination total plate count " measures the content of microorganism in microbial solution
For N, so that the amount of the microbial solution according to coating over platform assembly 10, calculates coating microorganism over platform assembly 10
Content N1, for example, determine microorganism in microbial solution content be 2.1*10^8CFU/ml, when by liquid-transfering gun absorption
Microbial solution when being 0.01ml, the content N1=2.1*10^8*0.01=2.1* of coating microorganism over platform assembly 10
10^6CFU.Optionally, in an application scenarios, the amount for being coated with microorganism over platform assembly 10 should be no less than 1*10^
5CFU。
Optionally, step S10 should be operated in gnotobasis, which can be desinfection chamber, aseptic operating platform etc.
The gnotobasis that device provides.
Optionally, in other embodiments, in order to improve the accuracy of testing result, further include before step S10
The step of sterilizing to platform assembly 10, which can be moist heat sterilization, hot air sterilization, chemical sterilization, high steam go out
The sterilization methods such as bacterium are placed in 121 DEG C of high-pressure steam sterilizing pan 30 minutes, with reality for example, will test plate 102, guide rail 101 etc.
Existing complete sterilizing.
S20: cleaning piece is fixed on the contact surface of slide assemblies.
Cleaning piece 30 is fixed on the contact surface 201 of slide assemblies 20, specifically, cleaning piece 30 is fixed on sliding block
On 202 contact surface 201, or cleaning piece 30 is coated on sliding block 202, wherein fixed form can be bonding or pass through
Fixture etc., herein with no restrictions.
S30: mobile slide assemblies, so that slide assemblies drive the microorganism on cleaning piece wiping platform assembly.
Mobile slide assemblies 20, so that slide assemblies 20 drive cleaning piece 30 to wipe the microorganism on platform assembly 10, tool
Body, mobile sliding block 202 passes through cleaning piece 30 from the microorganism coating layer in detection plate 102, it is to be understood that is moving
When movable slider 202, additional downward power should not be applied to sliding block 202, which refers in vertical direction in addition to cunning
Block 202 is to the power except the gravity of cleaning piece 30.
Optionally, sliding block 202 is allowed to move back and forth in detection plate 102, to simulate test case in actual use.
Optionally, when cleaning piece 30 has antipathogenic composition, in wiping process, antipathogenic composition will transfer to detection plate
On 102, therefore, after making sliding block 202 that cleaning piece 30 be driven to wipe the microorganism in detection plate 102, further include the steps that standing,
Specifically, sliding block 202 rests on 102 surface of detection plate for a period of time when sliding block 202 is after the surface of detection plate 102 is mobile,
The time is between 30s~15min.Certainly, when cleaning piece 30 has more antibacterial materials, which can be with longer.
S40: the quantity of remaining microorganism on detection platform component, to detect the effect of cleaning piece removal microorganism
Fruit.
The quantity N2 of remaining microorganism on detection platform component 10, specifically, can refer to " GB4789.2-2016 food
Microbiological Test total plate count measurement " method measurement detection plate 102 on remaining microorganism quantity N2.
Optionally, in order to guarantee the accuracy of testing result, the quantity N2 of remaining microorganism in measurement detection plate 102
When, it will test remaining microorganism on plate 102 and be transferred in particular solution, optionally, which is neutralizer, to disappear
Except antipathogenic composition, so that remaining antipathogenic composition is lost the effect to Antimicrobial and killing, prevent remaining antipathogenic composition pair
Microorganism count has an impact.Optionally, neutralizer can be phosphate buffer, sodium thiosulfate neutralizer, in Tween 80
With agent, 2% glutaraldehyde neutralizer, Iodophor neutralizer etc..
On measuring detection plate 102 after the quantity N2 of remaining microorganism, detect that cleaning piece 30 removes the effect of microorganism
Fruit, for example, be calculated from the formula cleaning piece 30 goes bacterium rate p1=(N1-N2)/N1*100%, optionally, can also calculate
Cleaning piece 30 removes bacterium activity rate r1=lgN1-lgN2 out.
Optionally, in an application scenarios, step S10 to S40 can also be repeated, cleaning piece 30 is calculated and goes bacterium rate or go bacterium
The average value of activity rate.
Optionally, in another embodiment, in order to it is more convenient and accurately detection wiping front platform component 10 on
The quantity of microorganism can also be coated with the microorganism of identical quantity in two detection plates 102 of platform assembly 10 respectively, detection
The quantity N3 of microorganism in one of them detection plate 102, mobile slide assemblies 20 drive cleaning piece 30 to wipe with slide assemblies 20
Microorganism in another detection plate 102, and detect in the detection plate 102 the quantity N2 of remaining microorganism, then cleaning piece 30
Bacterium rate p2=(N3-N4)/N3*100% is removed, bacterium activity rate r2=lgN3-lgN4 is removed, while bacterium rate can also be measured and gone
The average value of bacterium activity rate, specific method can be found in above embodiment, and details are not described herein.
0, Figure 10 is the flow diagram of another embodiment of detection method refering to fig. 1, this method comprises:
S50: the microorganism of identical quantity is respectively coated in two detection plates of platform assembly.
S60: successively moving slide assemblies, and control cleaning piece and test cleaning piece is successively driven to distinguish with slide assemblies
Wipe the microorganism in two detection plates.
Optionally, control cleaning piece is sterile white cotton, is tested in cleaning piece and any of the above-described embodiment
Cleaning piece 30 is identical, and details are not described herein.
Optionally, control needs the pretreatment by following steps with cleaning piece:
S601: configuration wetting agent: the dissolution of the sodium carbonate of Tween 80 and 5g is diluted with water to 1L.
S602: taking 2.5ml wetting agent and 2.5g sodium carbonate to be dissolved in water, be modulated into the cleaning solution of 5L, by size 1m*3m
Control be put into the cleaning solution of 5L with cleaning piece, boil 1 hour, change water and boil again 5 minutes, then place into 4~5L's
Stir about 5 minutes in cold water,
S603: the control in step S602 being dried with cleaning piece, is cut, and is air-dried after sterilizing.
S70: according to the quantity of remaining microorganism in two detection plates, test cleaning piece relative comparison cleaning piece is calculated
Go bacterium effect.
Specifically, if the quantity of remaining microorganism is N5 in the detection plate 102 wiped across by control with cleaning piece, warp
The quantity for crossing remaining microorganism in the detection plate 102 that test is wiped across with cleaning piece is N6, then test cleaning piece relative to
The bacterium rate of going of control cleaning piece is p3=(N5-N6)/N5*100%, removes bacterium activity value: r3=lgN5-lgN6, optionally, also
Bacterium rate can be measured and remove the average value of bacterium activity rate.
By the detection method in the embodiment, it is capable of detecting when removal of the test with wiping relative to control cleaning piece
The effect of microorganism.
Below with the detection device and the micro- life of detection method detection paper for kitchen removal in any of the above-described embodiment
The effect of object.The paper for kitchen is commercially available paper for kitchen, the no added sterilization in surface or antipathogenic composition.
The Escherichia coli 8099 of predetermined quantity and staphylococcus aureus ATCC6538 are respectively coated to two and are detected
The surface of plate 102.Specifically, preparing Escherichia coli 8099 and staphylococcus aureus ATCC6538 strain on aseptic operating platform
Plate draws 10ml dilution in strain planar surface with pipette, lawn is rinsed with liquid-transfering gun pressure-vaccum, under then rinsing
Bacterium solution is suitably diluted with dilution, and the concentration of original bacteria suspension is adjusted with nephelometry or spectrophotometry, original bacteria is hanged
The control of liquid concentration draws the 1ml concentration bacteria suspension and is transferred to the three of the broth bouillon of TSB containing 9ml in 1~9 × 10^8cfu/ml
Bacterium solution is formed in the bottle of angle, is mixed, the dilution is 0.9% sodium chloride solution (physiological saline).
10ml bacterium solution is drawn using liquid-transfering gun to be added dropwise in detection plate 102, is smeared immediately with pipette tips on 102 surface of detection plate
Uniformly, it is placed in incubator or the drying on aseptic operating platform, drying time rolls over paper for kitchen less than 40min according to need
It at suitable size, is coated on sliding block 202,50ml dilution is added dropwise in 102 surface of detection after the drying, and a hand pinches detection
Plate 102, for another hand by cleaning piece 30 along detection plate length direction, wiping 5 back and forth, i.e., passes through surface, wiping knot totally 10 times
Beam stops 30s.Detection plate 102 without the detection plate 102 of wiping and after wiping is put into respectively and is diluted containing 25ml
In the wide-mouth bottle of liquid, wide-mouth bottle is placed in shaking table, rises to 160r/min to revolving speed, 1min is shaken in timing, presses " GB4789.2-
2016 food microbiological examination total plate counts measurement " method respectively to without wiping detection plate 102 and through wiping
Detection plate 102 after wiping carries out total plate count counting, respectively N7 and N8.
Calculate removing bacterium rate p=(N7-N8)/N7*100% and averagely removing bacterium rate p0 for paper for kitchen.Specific detection knot
Fruit can be found in table 1.
1 paper for kitchen of table goes bacterium rate and averagely goes bacterium rate
It is in contrast to the prior art, detection device of the present invention is used to detect the effect of cleaning piece removal microorganism, packet
It includes: platform assembly, for being coated with microorganism；Slide assemblies, for driving cleaning piece to wipe platform assembly under the effect of external force
On microorganism；Wherein, slide assemblies include the contact surface contacted with platform assembly, and cleaning piece is fixed on the contact surface, when outer
When power acts on slide assemblies, slide assemblies drive cleaning piece movement, so that analog subscriber wipes platform assembly using cleaning piece,
To detect the effect of cleaning piece removal microorganism, by the detection device, it is able to detect the effect of cleaning piece removal microorganism.
Mode the above is only the implementation of the present invention is not intended to limit the scope of the invention, all to utilize this
Equivalent structure or equivalent flow shift made by description of the invention and accompanying drawing content, it is relevant to be applied directly or indirectly in other
Technical field is included within the scope of the present invention.
Claims (10)
1. a kind of detection device, for detecting the effect of cleaning piece removal microorganism characterized by comprising
Platform assembly, for being coated with the microorganism of predetermined amount；
Slide assemblies, for driving the cleaning piece to wipe the microorganism on the platform assembly under the effect of external force；
Wherein, the slide assemblies include the contact surface contacted with the platform assembly, and the cleaning piece is fixed on the contact
On face, when external force is when the slide assemblies, the slide assemblies drive the cleaning piece movement, so that analog subscriber makes
The platform assembly is wiped with the cleaning piece, to detect the effect that the cleaning piece removes the microorganism.
2. detection device according to claim 1, which is characterized in that
The platform assembly includes: guide rail and detection plate,
The guide rail is U-shaped, including the first bottom plate and the first side plate extended vertically upwards along first bottom plate two sides, institute
It states detection plate to be fixed on first bottom plate, the microorganism is coated in the detection plate, when the mobile sliding of external force
When component, the slide assemblies drive the cleaning piece to move in the detection plate along the direction that the guide rail limits.
3. detection device according to claim 2, which is characterized in that
The platform assembly further include: the first positioning plate and the second positioning plate,
First positioning plate and second positioning plate are fixed on first bottom plate, and are located at the detection plate
Along the front and rear sides of the slide assemblies direction of motion, so that the detection plate is clamped, the detection plate and first positioning
The junction of plate is equipped with notch section, and one end close to the detection plate of the notch section is equipped with inclined plane part.
4. detection device according to claim 3, which is characterized in that
Second positioning plate further includes fixed being equipped with location hole, the platform assembly along the direction that the slide assemblies move
Position bolt, second positioning plate is fixed on first bottom plate by the positioning bolt by the location hole, described fixed
The length in position hole is greater than the diameter of the positioning bolt, and when unclamping the positioning bolt, second positioning plate can be along institute
It is moved in the length range of the location hole in the direction for stating guide rail restriction.
5. detection device according to claim 2, which is characterized in that
The slide assemblies include: sliding block,
The sliding block is placed in the detection plate, and the sliding block includes the contact surface, and the cleaning piece is fixed on described connect
In contacting surface, when the mobile sliding block of external force, the sliding block drives the cleaning piece to move in the detection plate.
6. detection device according to claim 5, which is characterized in that
The slide assemblies further include: frame,
The frame is inverted u-shaped, and the frame includes the second bottom plate and extends vertically downward along second bottom plate two sides
Second side plate, second bottom plate are equipped with through-hole, and the frame is fastened on the guide rail, and the sliding block is accommodated in described logical
It in hole, is contacted with the detection plate, when the mobile frame of external force, the frame drives the sliding block to limit along the guide rail
Direction movement.
7. detection device according to claim 6, which is characterized in that
Along the direction of the frame movement, the frame is additionally provided with first baffle and second baffle, the first baffle and institute
The first end for stating second baffle is fixedly connected with the frame, the second end of the first baffle and the second baffle with it is described
First end is rectangular, when the sliding block is accommodated in the through-hole, the second end of the first baffle and the second baffle
With the shoe contact.
8. detection device according to claim 2, which is characterized in that
Further include: limit assembly, the limit assembly include the first limit plate and the second limit plate, first limit plate with
And second limit plate is inverted u-shaped, first limit plate and second limit plate are fastened on the described of the guide rail
On first side plate, when slide assemblies move to the position of first limit plate, second limit plate, first limit
Plate, second limit plate prevent the slide assemblies from continuing to move, so that the slide assemblies are in first limit
It is moved between plate and second limit plate.
9. a kind of detection method, which is characterized in that using detection device as claimed in any one of claims 1 to 8 detection wiping
The effect of object removal microorganism, which comprises
The microorganism of predetermined amount is coated on the platform assembly；
The cleaning piece is fixed on the contact surface of the slide assemblies；
The mobile slide assemblies, so as to drive the cleaning piece to wipe described micro- on the platform assembly for the slide assemblies
Biology；
The quantity of the remaining microorganism on the platform assembly is detected, to detect that the cleaning piece removes micro- life
The effect of object.
10. detection method according to claim 9, which is characterized in that
Before the microorganism for being coated with predetermined amount on the platform assembly, further includes: carried out to the platform assembly
Sterilization treatment；
The microorganism that predetermined amount is coated on the platform assembly, specifically includes:
The microorganism is configured to microbial solution, the microbial solution is coated on to the detection plate of the platform assembly
On；
The dry detection plate, so that the microorganism is fixed in the detection plate；
It is described that the cleaning piece is fixed on the contact surface of the slide assemblies, it specifically includes:
The cleaning piece is coated on the sliding block of the slide assemblies.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711081192.3A CN109749927A (en) | 2017-11-06 | 2017-11-06 | A kind of detection device and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711081192.3A CN109749927A (en) | 2017-11-06 | 2017-11-06 | A kind of detection device and detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109749927A true CN109749927A (en) | 2019-05-14 |
Family
ID=66400014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711081192.3A Pending CN109749927A (en) | 2017-11-06 | 2017-11-06 | A kind of detection device and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109749927A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102045418A (en) * | 2009-10-22 | 2011-05-04 | 鸿富锦精密工业（深圳）有限公司 | Self-cleaning device |
| CN104059962A (en) * | 2014-07-15 | 2014-09-24 | 光明乳业股份有限公司 | Sampling method and detection method for microorganisms on inner surface of hollow container |
| TWM509126U (en) * | 2015-06-29 | 2015-09-21 | Jian-Hua Yu | Wiping device |
-
2017
- 2017-11-06 CN CN201711081192.3A patent/CN109749927A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102045418A (en) * | 2009-10-22 | 2011-05-04 | 鸿富锦精密工业（深圳）有限公司 | Self-cleaning device |
| CN104059962A (en) * | 2014-07-15 | 2014-09-24 | 光明乳业股份有限公司 | Sampling method and detection method for microorganisms on inner surface of hollow container |
| TWM509126U (en) * | 2015-06-29 | 2015-09-21 | Jian-Hua Yu | Wiping device |
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