CN109650556A - The composite bacillus microbial inoculum and its application of ammonia nitrogen and nitrite in degradation water body - Google Patents
The composite bacillus microbial inoculum and its application of ammonia nitrogen and nitrite in degradation water body Download PDFInfo
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- CN109650556A CN109650556A CN201910063184.9A CN201910063184A CN109650556A CN 109650556 A CN109650556 A CN 109650556A CN 201910063184 A CN201910063184 A CN 201910063184A CN 109650556 A CN109650556 A CN 109650556A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
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Abstract
The present invention relates to the composite bacillus microbial inoculums and its application of ammonia nitrogen and nitrite in a kind of degradation water body, wherein composite bacillus microbial inoculum includes bacillus subtilis, bacillus licheniformis, colloid bacillus cereus, bacillus megaterium, bacillus amyloliquefaciens and auxiliary material, and preparation made of each component compounds by a certain percentage can be applied to the reparation of water ecological setting and the improvement of aquaculture system.Technical solution of the present invention is by utilizing bacillus subtilis, bacillus licheniformis, colloid bacillus cereus, bacillus megaterium, the respective physio-biochemical characteristics of bacillus amyloliquefaciens, metabolic pathway difference, so that them is had complementary functions, cooperates with and play a role, to inhibit that the source material of ammonia nitrogen The sub-salt can be generated, promote the rapid conversion of ammonia nitrogen The sub-salt, ammonia nitrogen The sub-salt in efficient degradation water body improves water quality.Compared to chemical classes, physics class ammonia nitrogen The sub-salt remover, microbial inoculum of the invention controls ammonia nitrogen The sub-salt from source, and the duration is long, without any toxicity and side effects.
Description
Technical field
The present invention relates to ammonia nitrogen and nitrite in microorganism and its application field more particularly to a kind of degradation water body to answer
Close gemma bacillus agent and its application.
Background technique
Expanding economy brings serious environmental problem, and water pollution is then wherein important one side.The activity of the mankind
The content of inorganic nitrogen in water body is caused to increase, ammonia nitrogen and nitrite containing high concentration for a long time in water body, to water ecology ring
Border tool has a significant impact.In addition, as high-density breeding scale constantly expands, while improving aquaculture products yield
It has brought many problems and drawback, such as excreta, the dead organisms residuum of feed residual bait, fish shrimp crab etc. and has deposited to pond bottom
Portion is dissolved in water body, these excessive a large amount of dissolved oxygens of organic matter decomposition and consumption lead to substrate, water hypoxia, and produce
Raw some harmful substances, such as ammonia nitrogen, nitrite, sulfide deteriorate substrate, water body.The deterioration of bottom material of pond for breeding, water quality, especially
It is ammonia nitrogen, nitrite it is exceeded, result in breeding water body physicochemical environment, ecological environment runs down, cause aquatic livestock
Eruptive epidemic disease and Large Scale Death accident frequently occur, and bring heavy losses to raiser.Therefore in water body ammonia nitrogen and
Nitrite, which is handled, is of great significance for aquaculture and water ecological setting reparation.
Removal currently for ammonia nitrogen in water body and nitrite mostly uses greatly at chemical oxidation, physical absorption and microorganism
The methods of reason, chemical oxidation and physical absorption can quickly remove ammonia nitrogen and nitrite in water body, but at high cost, continue
Time is short;Microorganism treatment is also widely used in water body processing, has the characteristics that duration length, stability, but existing rank
The most of water process probiotics environmental suitabilities of section are poor, and treatment effeciency is low, and processing cost is high.
In consideration of it, the present invention provide in a kind of efficient degradation water body the composite bacillus preparation of ammonia nitrogen and nitrite and
It is applied, it is desirable to provide the method for ammonia nitrogen and nitrite in quick, efficient, cheap, the stable degradation water body of one kind.
Summary of the invention
In order to overcome the above-mentioned deficiency of the prior art, the present invention provides ammonia nitrogen and nitrite in a kind of degradation water body
Composite bacillus preparation and its application, using the physio-biochemical characteristics of various bacillus, metabolic pathway difference, inhibition can be produced
The source material of ammonifying nitrogen The sub-salt promotes the rapid conversion of ammonia nitrogen The sub-salt, improves water quality, thus the ammonia nitrogen in efficient degradation water body
The sub-salt;The preparation duration of the invention is long, without any toxicity and side effects.
To achieve the goals above, the present invention is achieved by the following technical solutions:
The first purpose of this invention is to propose the composite bacillus of ammonia nitrogen and nitrite in a kind of degradation water body
Microbial inoculum, the composite bacillus microbial inoculum include bacillus subtilis, bacillus licheniformis, colloid bacillus cereus, huge gemma
Bacillus, bacillus amyloliquefaciens and auxiliary material.
Further, each component according to parts by weight, 10-15 parts of bacillus subtilis, 20-30 parts of bacillus licheniformis,
5-10 parts of colloid bacillus cereus, 20-30 parts of bacillus megaterium, 20-30 parts of bacillus amyloliquefaciens, 5-10 parts of auxiliary material.
Further, each component according to parts by weight, 12-14 parts of bacillus subtilis, bacillus licheniformis 24-28
Part, 7-8 parts of colloid bacillus cereus, 24-28 parts of bacillus megaterium, 24-26 parts of bacillus amyloliquefaciens, 6-8 parts of auxiliary material.
Further, the auxiliary material is medical stone and talcum powder, and weight ratio is 1.5~3:1.
Further, the microbial inoculum is that pulvis, tablet, granule is made using composite bacillus as active constituent
Dosage form.
Second object of the present invention is to propose ammonia nitrogen and nitrite in degradation water body described in any of the above embodiments
Composite bacillus microbial inoculum is repairing the application in water ecological setting.
Further, the composite bacillus microbial inoculum is applied to reduce water nitrite, ammonia-nitrogen content and improves water
Body transparency.
Third object of the present invention is to propose ammonia nitrogen and nitrite in degradation water body described in any of the above embodiments
Composite bacillus microbial inoculum is adjusting the application in aquaculture system.
Further, the composite bacillus microbial inoculum is applied to reduce water nitrite, ammonia-nitrogen content and inhibits water
Produce cultivated animals bacteriosis.
Fourth object of the present invention is to propose ammonia nitrogen and nitrite in degradation water body described in any of the above embodiments
The application method of composite bacillus microbial inoculum is calculated the dosage of composite bacillus microbial inoculum in the ratio of 250-300g/ mus/rice,
Composite bacillus microbial inoculum is added in the brown sugar water of 6-15 times of volume, oxygenation activates 1-4 hours, uniform when fine
It splashes in the water surface;The mass concentration of the brown sugar water is 5%.
When being applied to adjust water quality in aquaculture by the microbial inoculum and repair the natural waters such as river, lake, in each component:
The extracellular enzymes such as a large amount of cellulase of bacillus subtilis secretion, amylase and protease, can sufficiently decompose
Larger molecular organics in water body reduce the source material for generating ammonia nitrogen and nitrite.
Bacillus licheniformis can secrete a large amount of protease, lipase and amylase, for promote in water body residual bait and
The decomposition of excrement reduces the generation of water body ammonia nitrogen and The sub-salt;Bacillus licheniformis is also colonized in the enteron aisle of aquatic livestock, promotes intestines
The digestion and absorption in road adjusts intestinal bacilli illness, reduces the generation of the pollutants such as body ammonia nitrogen, hydrogen sulfide.
Colloid bacillus cereus is used to promote to be not easy in water body the release of the sylvite utilized by algae, water plant etc., simultaneously
Also the release for promoting sylvite in bed mud, effectively facilitates the growth of hydrobiontic alga, plant, to increase water body dissolved oxygen, promotes ammonia nitrogen
The sub-salt improves poisonous and harmful substance accumulation, improves aquatic environment to the rapid conversion of nitrate
Bacillus megaterium is for promoting phosphatic release in water body, particularly with some phosphorus ore containings not soluble in water
Matter, bacillus megaterium can secrete the release that a large amount of metabolites promote P elements in phosphorus ore containing matter, promote algae and water,
The growth of plant and zooplankter adjusts water ecology balance, improves the accumulation of ammonia nitrogen in water body, The sub-salt
Bacillus amyloliquefaciens effectively inhibit harmful in water body for generating the lipopeptids substance such as a large amount of iturin
The growth of microorganism reduces harmful microorganism and decomposes the ammonia nitrogen The sub-salt generated.
Medical stone is a kind of natural aggregate of multi mineral with biological effect, with extremely strong adsorptivity, molten
Property and bioactivity out can be used as absorption microorganism carrier, and microorganism is allowed to have better preservation condition, extend product and guarantee the quality
Phase.Simultaneously because medical stone has good adsorptivity, there is very strong suction to larger molecular organics, the pollutant in water body
Attached effect can be very good the effect for improving water quality.
Talcum powder equally has very strong absorption property, can be used as the carrier of microorganism adsorption, and microorganism is allowed to have more preferably
Preservation condition can play the role of purifying water furthermore it is stronger to the pollutant adsorption capacity in water body.Talcum powder with
The absorption property of medical stone is variant, by combining the two, using the synergistic effect of the two, can be very good to allow of the invention
The product holding time is longer, plays good water purification.
Therefore, compared with prior art, the beneficial effects of the present invention are:
Technical solution of the present invention is by utilizing bacillus subtilis, bacillus licheniformis, colloid bacillus cereus, huge bud
The respective physio-biochemical characteristics of spore bacillus, bacillus amyloliquefaciens, metabolic pathway difference reduce and generate ammonia nitrogen The sub-salt source material
Generation, promote ammonia nitrogen The sub-salt rapid conversion, improve water quality, thus the ammonia nitrogen The sub-salt in efficient degradation water body.And it compares
In chemical classes, physics class ammonia nitrogen The sub-salt remover, biological removal agent controls ammonia nitrogen The sub-salt from source, and the duration is more
It is long, without any toxicity and side effects.
Specific embodiment
It shows that example illustrates certain embodiments of the present invention, and should not be construed as limiting model of the invention
It encloses.Present disclosure can be improved from material, method and reaction condition simultaneously, all these improvement should all
It falls within spirit and scope of the invention.
The composite bacillus microbial inoculum of ammonia nitrogen and nitrite in degradation water body of the invention, including bacillus subtilis,
Bacillus licheniformis, colloid bacillus cereus, bacillus megaterium, bacillus amyloliquefaciens and auxiliary material, each component is in parts by weight
Meter, 10-15 parts of bacillus subtilis, 20-30 parts of bacillus licheniformis, 5-10 parts of colloid bacillus cereus, bacillus megaterium 20-
30 parts, 20-30 parts of bacillus amyloliquefaciens, 5-10 parts of auxiliary material, preferably bacillus subtilis 12-14 parts, bacillus licheniformis
24-28 parts, 7-8 parts of colloid bacillus cereus, 24-28 parts of bacillus megaterium, 24-26 parts of bacillus amyloliquefaciens, auxiliary material 6-8
Part.Wherein auxiliary material is medical stone and talcum powder, and weight ratio is 1.5~3:1.The microbial inoculum is using composite bacillus as work
Property ingredient, is made the dosage form of pulvis, tablet, granule.
Composite bacillus microbial inoculum of the invention can be applied to the reparation of water ecological setting and changing for aquaculture system
It is kind, it is specifically applicable to reduce water nitrite, ammonia-nitrogen content, improves water transparency, inhibits aquiculture animal bacterium
Property disease etc..The application method of the composite bacillus microbial inoculum of ammonia nitrogen and nitrite in the degradation water body, by 250-
The ratio of 300g/ mus/rice calculates the dosage of composite bacillus microbial inoculum, and composite bacillus microbial inoculum is added to 6-15 times of volume
Brown sugar water in, oxygenation activate 1-4 hours, uniformly splash when fine in the water surface;The quality of the brown sugar water is dense
Degree is 5%.
Illustrate below by way of specific embodiment, bacterial strain involved in embodiment is the limited public affairs of Wuhan Feng Tian biotechnology
Department is isolated from natural environment;Bacterium raw material involved in embodiment is that Wuhan Feng Tian Biotechnology Co., Ltd sells
Microbial germ powder;Preparation in embodiment 1-4 is the pulvis as made of a variety of bacterium powder and auxiliary material compounding.
Embodiment 1:
1, it is formulated
The present embodiment provides the composite bacillus preparation of ammonia nitrogen and nitrite in a kind of efficient degradation water body, formulas
In include bacillus subtilis 100g, bacillus licheniformis 200g, colloid bacillus cereus 50g, bacillus megaterium 300g, Xie Dian
Afnyloliquefaciens 300g, medical stone 30g and talcum powder 20g.
2, application method
The usage of bacillus preparation are as follows: the dosage that bacillus preparation is calculated in the ratio of 250g/ mus/rice is added to 6
In the brown sugar water (quality amount concentration is 5% brown sugar) of times volume, activated 2 hours with nickel base alloy powder spray coating mixing rectangular or agitating device oxygenation,
The effect of degradation of ammonia nitrogen and nitrite can be played in the water surface by uniformly splashing when fine.
Embodiment 2:
1, it is formulated
The present embodiment provides the composite bacillus preparation of ammonia nitrogen and nitrite in a kind of efficient degradation water body, formulas
In include bacillus subtilis 120g, bacillus licheniformis 240g, colloid bacillus cereus 100g, bacillus megaterium 200g, solution
Bacillus amyloliquefaciens 250g, medical stone 60g and talcum powder 20g.
2, application method
The usage of bacillus preparation are as follows: the dosage that bacillus preparation is calculated in the ratio of 250g/ mus/rice is added to
In the brown sugar water (quality amount concentration is 5% brown sugar) of 10 times of volumes, activated 1 hour with nickel base alloy powder spray coating mixing rectangular or agitating device oxygenation,
The effect of degradation of ammonia nitrogen and nitrite can be played in the water surface by uniformly splashing when fine.
Embodiment 3:
1, it is formulated
The present embodiment provides the composite bacillus preparation of ammonia nitrogen and nitrite in a kind of efficient degradation water body, formulas
In include bacillus subtilis 140g, bacillus licheniformis 280g, colloid bacillus cereus 70g, bacillus megaterium 240g, Xie Dian
Afnyloliquefaciens 260g, medical stone 75g and talcum powder 25g.
2, application method
The usage of bacillus preparation are as follows: the dosage that bacillus preparation is calculated in the ratio of 250g/ mus/rice is added to
In the brown sugar water (quality amount concentration is 5% brown sugar) of 12 times of volumes, activated 3 hours with nickel base alloy powder spray coating mixing rectangular or agitating device oxygenation,
The effect of degradation of ammonia nitrogen and nitrite can be played in the water surface by uniformly splashing when fine.
Embodiment 4:
1, it is formulated
The present embodiment provides the composite bacillus preparation of ammonia nitrogen and nitrite in a kind of efficient degradation water body, formulas
In include bacillus subtilis 150g, bacillus licheniformis 300g, colloid bacillus cereus 80g, bacillus megaterium 280g, Xie Dian
Afnyloliquefaciens 200g, medical stone 40g and talcum powder 20g.
2, application method
The usage of bacillus preparation are as follows: the dosage that bacillus preparation is calculated in the ratio of 250g/ mus/rice is added to
In the brown sugar water (quality amount concentration is 5% brown sugar) of 15 times of volumes, activated 4 hours with nickel base alloy powder spray coating mixing rectangular or agitating device oxygenation,
The effect of degradation of ammonia nitrogen and nitrite can be played in the water surface by uniformly splashing when fine.
Embodiment 5:
8, the Jiangxi Province raiser intensive culture pool is selected, water quality situation is close, Water quality ammonia nitrogen 0.2-0.3mg/L, nitrite
0.3-0.5mg/L.It is divided into four groups, every group is set up control group and experimental group, and gained microbial inoculum product in embodiment 1-4 is pressed institute respectively
Method is stated applied in the pool, detection ammonia nitrogen and nitrite change with time, and the results are shown in Table 1.
As it can be seen from table 1 ammonia nitrogen can be dropped to 0 by the experimental group in embodiment when second day, and control group
Ammonia nitrogen keep stablize, on the rise, the experimental group in embodiment can drop to the nitrite in water body in third day
0, control group then keeps stable.Experimental group can significantly improve water transparency, and the transparency of control group has decreasing trend.So
The composite bacillus preparation prepared in embodiment 1-4 can ammonia nitrogen and nitrite in efficient degradation water body, can be with pollution degradation
Object improves aquatic environment, and the duration is longer, provides the environment of health for cultivated animals, improves cultivated animals cultivation effect
Rate.
Table 1 breeding water body water quality detection result (ammonia nitrogen, NO2 -Unit is mg/L, and transparency unit is cm)
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite in a kind of degradation water body, it is characterised in that: the compound gemma
Bacillus microbial inoculum includes bacillus subtilis, bacillus licheniformis, colloid bacillus cereus, bacillus megaterium, solution starch gemma bar
Bacterium and auxiliary material.
2. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite, special in a kind of degradation water body according to claim 1
Sign is: each component according to parts by weight, 10-15 parts of bacillus subtilis, 20-30 parts of bacillus licheniformis, colloid gemma bar
5-10 parts of bacterium, 20-30 parts of bacillus megaterium, 20-30 parts of bacillus amyloliquefaciens, 5-10 parts of auxiliary material.
3. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite, special in a kind of degradation water body according to claim 2
Sign is: each component according to parts by weight, 12-14 parts of bacillus subtilis, 24-28 parts of bacillus licheniformis, colloid gemma bar
7-8 parts of bacterium, 24-28 parts of bacillus megaterium, 24-26 parts of bacillus amyloliquefaciens, 6-8 parts of auxiliary material.
4. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite, special in a kind of degradation water body according to claim 1
Sign is: the auxiliary material is medical stone and talcum powder, and weight ratio is 1.5~3:1.
5. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite, special in a kind of degradation water body according to claim 1
Sign is: the microbial inoculum is that the dosage form of pulvis, tablet, granule is made using composite bacillus as active constituent.
6. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite is being repaired in degradation water body as described in any one in claim 1-5
Application in multiple water ecological setting.
7. the application of the composite bacillus microbial inoculum of ammonia nitrogen and nitrite in degradation water body according to claim 6,
It is characterized in that, the composite bacillus microbial inoculum is applied to reduce water nitrite, ammonia-nitrogen content and improves water transparency.
8. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite is being adjusted in degradation water body as described in any one in claim 1-5
Save the application in aquaculture system.
9. the application of the composite bacillus microbial inoculum of ammonia nitrogen and nitrite in degradation water body according to claim 8,
It is characterized in that, the composite bacillus microbial inoculum is applied to reduce water nitrite, ammonia-nitrogen content and inhibits aquaculture dynamic
Object bacteriosis.
10. the composite bacillus microbial inoculum of ammonia nitrogen and nitrite in degradation water body as described in any one in claim 1-5
Application method, which is characterized in that the dosage that composite bacillus microbial inoculum is calculated in the ratio of 250-300g/ mus/rice, by compound bud
Spore bacillus microbial inoculum is added in the brown sugar water of 6-15 times of volume, and oxygenation activates 1-4 hours, is uniformly splashed when fine in water
Face;The mass concentration of the brown sugar water is 5%.
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CN111100830A (en) * | 2020-03-09 | 2020-05-05 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
CN112522163A (en) * | 2020-12-25 | 2021-03-19 | 南京升鳌生物环保科技有限公司 | Microbial repairing agent for eliminating old green water and preparation method and application thereof |
CN113929221A (en) * | 2021-11-22 | 2022-01-14 | 宁波大学 | Microbial composite flora for treating aquaculture tail water |
CN116515710A (en) * | 2023-05-26 | 2023-08-01 | 江苏海洋大学 | Heterotrophic nitrifying bacteria and microbial inoculum, preparation method and application thereof |
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CN116515710A (en) * | 2023-05-26 | 2023-08-01 | 江苏海洋大学 | Heterotrophic nitrifying bacteria and microbial inoculum, preparation method and application thereof |
CN116515710B (en) * | 2023-05-26 | 2024-04-05 | 江苏海洋大学 | Heterotrophic nitrifying bacteria and microbial inoculum, preparation method and application thereof |
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