CN109626584A - A kind of method of microalgae processing sauce waste water - Google Patents

A kind of method of microalgae processing sauce waste water Download PDF

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Publication number
CN109626584A
CN109626584A CN201811631894.9A CN201811631894A CN109626584A CN 109626584 A CN109626584 A CN 109626584A CN 201811631894 A CN201811631894 A CN 201811631894A CN 109626584 A CN109626584 A CN 109626584A
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microalgae
waste water
sauce
sauce waste
culture
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周文广
宋汉武
芦骞
韩佩
李晶晶
冷立健
黎俊
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Nanchang University
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Nanchang University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters

Abstract

A kind of method of microalgae processing sauce waste water, the microalgae of salt tolerant resistant is obtained especially by screening, it is then added in culture medium and carries out High Density Cultivation, it will be in the microalgae cell access sauce waste water of harvest, shaken cultivation is carried out under illumination condition, due to can largely consume the substances such as organic matter, nitrogen phosphorus in waste water in micro algae growth metabolic process, to reduce the pollutant concentration in waste water, achieve the purpose that purify waste water.The method of the present invention is easy to operate, equipment investment is small, greatly reduces the processing cost of waste water, meets the requirement of microalgae industryization processing wastewater application, has many advantages, such as that efficient, environmental protection, comprehensive utilization ratio are high.The nutriments such as organic matter, nitrogen phosphorus in microalgae efficient absorption sauce waste water and the biomass for being converted into high added value, the microalgae cell of harvest can be used for subsequent bioenergy preparation and feed, bait application etc., economic well-being of workers and staff is not only increased, and adequately achieves recycling for resource.

Description

A kind of method of microalgae processing sauce waste water
Technical field
The present invention relates to a kind of methods of microalgae processing sauce waste water, the microalgae of salt tolerant resistant are obtained by screening, then It is added in culture medium and carries out High Density Cultivation, by the microalgae cell access sauce waste water of harvest, carried out under illumination condition Shaken cultivation, the substances such as a large amount of organic matter, nitrogen phosphorus consumed in waste water reach net to reduce the pollutant concentration in waste water Change the purpose of waste water, the microalgae biomass of harvest can be used for subsequent resource utilization, particularly belong to technical field of environmental management.
Background technique
With the development of the social economy, the raising of living standards of the people, people are higher and higher for the pursuit of food taste, Soy sauce is continuously increased as a kind of traditional flavoring agent, yield, while the fast development of soy sauce manufacturing enterprise, is also produced big The sauce waste water of amount.Since soy sauce production process is related to multiple techniques, waste component is complicated, water quality and quantity is unstable, coloration It is high, salinity is big, taste weight, processing difficulty is larger, is a kind of high concentrated organic wastewater.If not being pocessed or handling row not up to standard Serious water environment pollution will be caused by putting.
Sauce waste water processing at present mostly uses traditional biological treatment, but its complicated component, coloration are high, and microorganism is difficult to Adapt to, and its high salinity can growth to Microbial Communities in Activated Sludge and nutritional ingredient using generating inhibiting effect, Jin Erying Ring water treatment effect.In addition, traditional biological facture, there is also equipment investment is big, sludge yield is more, processing cost is high The problems such as, economic benefit is unobvious.Currently, using traditional aerobic/anaerobic microbiological processes processing sauce waste water, there is all More contradictions, one side sewage treatment expense is higher, limits enterprise getting profit；Cannot there is nutriment in another aspect sewage Effect recycles, and is a kind of waste of resource.A kind of patent " soy sauce brewing wastewater processing system " of the applications such as Tan Yongqiao (CN201720787158.7) in, it is useless that soy sauce is handled at one big system by combination traditional physical chemistry and bioprocess technology Water, system is excessively huge, assembled unit is excessive, equipment investment is big, energy consumption is high, processing cost is high, the reagent that adds will cause The problems such as secondary pollution, difficult excess sludge, keeps its processing system economic benefit unobvious.Patent " the sauce of the applications such as Lv Si Hao In the processing method and processing system of oily brewery industry waste water " (CN201210039089.3), combination anaerobism, anoxic biochemistry pool, The different units such as the aerobic pond MBR, coagulative precipitation tank, oxidative decoloration pond handle sauce waste water, and a combination thereof unit is more, throw It is big to provide cost, Pollutants in Wastewater cannot be removed simultaneously, the aluminium polychloride and sodium hypochlorite being added in sedimentation basin and oxidation pond Equal drugs may further polluted-water, the sludge yield after handling waste water is big, also needs to be handled, cannot achieve waste water The recycling of middle nutriment is unsatisfactory for resource circulation utilization theory.
Microalgae is a kind of important photosynthetic/mixotroph, is different from bacterium, and microalgae can both be absorbed by metabolic activity For the nutriments such as organic matter and nitrogen phosphorus in waste water for own growth, the biomass of harvest can be used for fodder and bait, biology combustion again Material and the production of other high value added products etc..On the one hand the recycling for realizing resource, on the other hand turns waste into wealth, Create additional economic benefit.About microalgae processing waste water research reported it is numerous, studies at home and abroad show that, the sheet of screening Ground microalgae such as chlorella, Dunaliella salina etc. is to some waste water such as concentration property municipal wastewater, Pig raising wastewater, garbage leachate wastewater There is good removal effect, so that the nutritive index in sewage be effectively reduced, reaches purpose of purifying the water quality.Furthermore some microalgaes With salt-tolerant trait, can survive under hypersaline environment.And the organic matter of sauce waste water middle and high concentration can be provided for microalgae and be filled The nutrient source of foot, the microalgae biomass after harvesting can be further used for subsequent resource utilization, adequately achieve resource It recycles.Microdisk electrode and cost for wastewater treatment can not only be effectively reduced in microalgae processing sauce waste water mode, biomass Using additional economic well-being of workers and staff can also be brought.Therefore, there is biggish development potentiality using microalgae processing sauce waste water, is a kind of Novel wastewater treatment method.
Summary of the invention
The present invention is for equipment investment is big, cumbersome, processing cost is high, is also easy to produce existing for sauce waste water processing method The problems such as secondary pollution, resource do not recycle, propose it is a kind of it is novel be simple and efficient, low-cost microalgae processing sauce waste water Method, the microalgae being related to belong to both can be carried out photoautotrophy and also can using organic carbon carry out chemoheterotrophy algae, due to it It can be absorbed in sauce waste water that a large amount of nutriments such as organic carbon and nitrogen phosphorus are for own growth in the metabolic process, to remove Pollutant in waste water achievees the purpose that purify waste water, while the microalgae biomass harvested can be used for subsequent resource utilization.
The method of present invention microalgae processing sauce waste water a kind of the following steps are included:
Step 1: microalgae isolates and purifies
In 24 orifice plates, enrichment culture is carried out to the microalgae in natural water with autotrophy culture medium, algae is grown and falls behind, then pass through weight Multiple plate streaking separation obtains purebred algae strain；
Autotrophy condition of culture: temperature is controlled at 20-45 DEG C, is passed through air or air and CO2Mixed gas, ventilatory capacity 80- 120L/h, CO2Concentration 0.9%-3%(L/L), using 10-200 μm of ol.m-2s-1Solar radiation, pH value control is total to cultivate in 5-9 Time 120-200h；
Step 2: the screening of salt tolerant resistant microalgae
According to volume ratio, sauce waste water is diluted to 1/20,1/10, the 1/5 of original concentration respectively with distilled water, thereafter access step Rapid 1 isolated algae strain, is placed in illumination shaking table and is cultivated, in 28 ± 5 DEG C, 120 ± 20rpm, 10-200 μm of ol.m-2s-1's Under the conditions of cultivate 96-120h, the microalgae of salt tolerant resistant is filtered out, and be seeded to autotrophy culture medium solid slope, in low temperature and irradiance It is saved in incubator；
Step 3: the High Density Cultivation of microalgae cell
Step 2 inclined-plane culture algae is seeded to bioreactor to cultivate, in the Heterotrophic culture base of addition organic carbon source High Density Cultivation, until cell log growth period, cell density reaches 106-1010A/ml or more；
The high-density culturing condition: organic carbon source concentration of glucose 0.01-200 g.L-1, it is passed through the ventilatory capacity of air 150-250L/h, using 5-40 μm of ol.m-2s-1Solar radiation, pH value control is total to train in 5-9, hunting speed 100-150rpm The time is supported between 100-150h；
Step 4: the harvest of microalgae cell
The microalgae cell of step 3 High Density Cultivation is centrifuged with the revolving speed of 800-4000rpm, the microalgae cell of harvest It is harvested with after aseptic water washing 2-3 times；
Step 5: the purification of culture and waste water of the microalgae cell in sauce waste water
The microalgae cell that step 4 is harvested is transferred to progress illumination shaken cultivation in the sauce waste water of different extension rates, absorbs Each nutriment in sauce waste water reduces pollutant concentration in sauce waste water, purifies sauce waste water；
The condition of culture: sauce waste water distilled water or tap water according to 5 times of volume ratio dilution, 2 times of dilution or do not dilute, The initial inoculum of microalgae cell is 106-108A/ml, 20-45 DEG C of temperature, hunting speed 100-150rpm, using 10-200 μ mol.m-2s-1Solar radiation, total incubation time is between 1-15d；
Step 6: the biomass recycling use of microalgae
Recycling step 5 purify sauce waste water after microalgae, to microalgae carry out biomass recycling use, be used as feed, bait or Bioenergy raw material；
The autotrophy culture medium prescription are as follows: K2HPO4·3H2O0.04g/L, MgSO4·7H2O 0.075g/L, CaCl2· 2H2O 0.036g/L, citric acid 0.006g/L, ferric citrate 0.006g/L, EDTA 0.001g/L, NaNO31.5g/L Na2CO30.02g/L, A5 microelement 1.5ml/L；Wherein A5 liquid microelement forms: H3BO32.86g/L MnCl2·4H2O 1.81g/L ZnSO4·7H2O0.222g/L, NaMoO4·2H2O0.39g/L, CuSO4·5H2O0.079g/L and CoCl2·6H2O 0.05g/L；
The Heterotrophic culture based formulas: it is 1-20 that organic carbon source to initial reduction sugar concentration is added in autotrophy culture medium g.L-1
The microalgae includes but is not limited to Chlorella, barrel mast Trentepohlia, spirulina, diatom, diamond shape algae, splits pot algae, Du Shi algae Category, scenedesmus, Nannochloropsis oculata, Chlamydomonas, flat algae or empty ball Trentepohlia.
The organic carbon includes but is not limited to monosaccharide, disaccharides, polysaccharide, organic acid or alcohols.
Beneficial effects of the present invention: 1, handling sauce waste water using algae for the first time, is based on micro algae growth metabolic characteristic, due to Part microalgae can photoautotrophy can use organic carbon heterotrophism, and strong environmental adaptability, in the case where there is light, microalgae again It can be grown using organic matter, the nitrogen phosphorus etc. in sauce waste water, substantially reduce the pollutant concentration in waste water, reach net Change the purpose of waste water, thus develop it is a kind of it is novel be simple and efficient, be low in cost, the economic benefit significantly soy sauce based on microalgae it is useless Method for treating water.2, sauce waste water is easy to operate, equipment investment is small for algae processing, greatly reduces the processing cost of waste water and micro- The toxigenic capacity of algae meets the requirement of microalgae industryization processing wastewater application, has efficient, environmental protection, versatility height etc. Advantage.3, the nutriments such as the organic matter in microalgae efficient absorption sauce waste water, nitrogen phosphorus and it is converted into itself biomass, harvest Microalgae cell can be used for the preparation of subsequent bioenergy and feed, bait application etc., not only increase additional economic well-being of workers and staff, And adequately achieve the recycling of resource.
Detailed description of the invention
Fig. 1 is growing state of the different initial inoculum chlorellas of the present invention in different extension rate sauce waste waters；
Fig. 2 is the different initial inoculum chlorellas of the present invention to ammonia nitrogen removal situation in different extension rate sauce waste waters；
Fig. 3 is that the different initial inoculum chlorellas of the present invention remove situation to COD in different extension rate sauce waste waters；
Fig. 4 is that the different initial inoculum chlorellas of the present invention remove situation to total nitrogen in different extension rate sauce waste waters；
Fig. 5 is the different initial inoculum chlorellas of the present invention to phosphorus ligands situation total in different extension rate sauce waste waters；
Fig. 6 is growing state of the spirulina of the present invention in different extension rate sauce waste waters；
Fig. 7 is removal situation of the spirulina of the present invention to ammonia nitrogen in different extension rate sauce waste waters；
Fig. 8 is removal situation of the spirulina of the present invention to COD in different extension rate sauce waste waters；
Fig. 9 is removal situation of the spirulina of the present invention to total nitrogen in different extension rate sauce waste waters.
Specific embodiment
The present invention will be described further by following instance.
Embodiment 1:
Salt tolerant resistant algae strain-chlorella (Chlorella pyrenoidosa) separation screening in local water environment, algae strain Heterotrophic growth can be carried out in autophyting growth under photosynthesis and using organic carbon, while can also be carried out under illumination condition And health is long.Its adaptation situation and growth characteristics in sauce waste water is as shown in Figure 1.
The autotrophy culture medium prescription that above-mentioned microdisk electrode is related to are as follows: K2HPO4·3H2O0.04g/L, MgSO4·7H2O 0.075g/L, CaCl2·2H2O0.036g/L, citric acid 0.006g/L, ferric citrate 0.006g/L, EDTA 0.001g/L, NaNO31.5g/L, Na2CO30.02g/L, A5Liquid microelement 1.5ml/L, wherein A5Liquid microelement composition: H3BO32.86g/ L, MnCl2·4H2O1.81g/L, ZnSO4·7H2O0.222g/L, NaMoO4·2H2O0.39g/L, CuSO4·5H2O 0.079g/ L, CoCl2·6H2O0.05g/L.
Heterotrophic culture based formulas is same as above, and it is 0.1-200 g/L, preferably 15g/L that organic carbon source to initial reduction sugar concentration, which is added,.
Sauce waste water, which needs to remove in waste water by preprocessing process such as precipitating, filtering or centrifugations before cultivating microalgae, to be existed Part large particle.
Water sample is acquired in natural environment and the mixing of culture medium ratio carries out enrichment culture, is grown algae and is fallen behind oese weight Isolated microalgae is accessed in the sauce waste water of different dilution ratios and is grown, sieved up to isolating purebred microalgae by multiple plate streaking Choosing obtains good, the adaptable chlorella of upgrowth situation.Then chlorella is seeded to shaking table culture in 250mL shaking flask, temperature Degree control is at 28 DEG C ± 5 DEG C；After cell enters logarithmic growth latter stage, culture medium of the access containing organic carbon is filled in biological respinse Set middle carry out High Density Cultivation.Biological reaction apparatus includes shaking flask, bioreactor, light fermentor and open culturing pond etc.. It is cultivated under above-mentioned suitable condition, cell density reaches 10 in logarithmic growth phase6-1010.Finally take logarithmic growth later period or stabilization The chlorella cells of phase, with the centrifuge slow-speed of revolution (800-4000rpm) centrifugation harvested, the chlorella cells of harvest spend from Sub- water rinse 2-3 times after by certain initial inoculum be added in the sauce waste water of different dilution ratios illumination 3000lux, 120rpm, it cultivates at 28 DEG C, chlorella can efficiently utilize nutriment, the purification waste water in sauce waste water, tool as the result is shown Body is shown in Fig. 2, Fig. 3, Fig. 4, Fig. 5.
Embodiment 2:
Salt tolerant resistant algae strain-spirulina (Spirulina) acquisition isolated and purified by laboratory early stage.Algae strain also adapts to sauce The organic matter of oily Coal Gas Washing Cycling Water.Its adaptation situation and growth characteristics in sauce waste water is as shown in Figure 6.
The culture medium prescription that above-mentioned microdisk electrode is related to are as follows: NaHCO316.8g/L NaNO32.5g/L, NaCl1.0g/L, K2HPO40.5g/L, K2SO41.0g/L, MgSO4·7H2O0.2g/L, CaCl2·2H2O0.04g/L, EDTA0.08g/L, A5It is micro Element 1ml/L, B6Microelement 1ml/L.Wherein A5Liquid microelement composition: H3BO32.86g/L MnCl2·4H2O1.81g/ L, ZnSO4·7H2O0.222g/L, NaMoO4·2H2O0.39g/L, CuSO4·5H2O 0.079g/L, CoCl2·6H2O0.05g/ L；B6Liquid microelement composition: NH4VO322.9mg/L, CrK (SO4)2·12H2O 96.0mg/L, NiSO4·6H2O47.8mg/L, NaWO4·2H2O17.9mg/L, Co (NO3)2·6H2O 44.0mg/L, Ti (SO4)2 40.0mg/L。
Sauce waste water, which needs to remove in waste water by preprocessing process such as precipitating, filtering or centrifugations before cultivating microalgae, to be existed Part large particle.
The spirulina single colonie being grown on solid medium is seeded to shaking table culture in 250mL shaking flask, temperature control At 28 DEG C ± 5 DEG C；After cell enters logarithmic growth latter stage, accesses in biological reaction apparatus and carry out High Density Cultivation.It is biological anti- Answering device includes shaking flask, bioreactor, light fermentor and open culturing pond etc..It is cultivated under illumination condition, cell density Reach 10 in logarithmic growth phase6-1010.The spirulina cells for finally taking logarithmic growth later period or stationary phase, with the centrifuge slow-speed of revolution (800-4000rpm) is harvested by centrifugation, and the spirulina cells of harvest press certain initial inoculum after being rinsed 2-3 times with deionized water (OD560≈ 0.4) it is added in the sauce waste water of different dilution ratios and is cultivated at illumination 3000lux, 120rpm, 28 DEG C, as a result show Show that spirulina can be efficiently using the nutriment in sauce waste water, purification waste water situation is shown in Fig. 7, Fig. 8, Fig. 9.

Claims (3)

1. a kind of method of microalgae processing sauce waste water, it is characterised in that: the method the following steps are included:
Step 1: microalgae isolates and purifies
In 24 orifice plates, enrichment culture is carried out to the microalgae in natural water with autotrophy culture medium, algae is grown and falls behind, then pass through weight Multiple plate streaking separation obtains purebred algae strain；
Autotrophy condition of culture: temperature is controlled at 20-45 DEG C, is passed through air or air and CO2Mixed gas, ventilatory capacity 80- 120L/h, CO2Concentration 0.9%-3%(L/L), using 10-200 μm of ol.m-2s-1Solar radiation, pH value control is total to cultivate in 5-9 Time 120-200h；
Step 2: the screening of salt tolerant resistant microalgae
According to volume ratio, sauce waste water is diluted to 1/20,1/10, the 1/5 of original concentration respectively with distilled water, thereafter access step Rapid 1 isolated algae strain, is placed in illumination shaking table and is cultivated, in 28 ± 5 DEG C, 120 ± 20rpm, 10-200 μm of ol.m-2s-1's Under the conditions of cultivate 96-120h, the microalgae of salt tolerant resistant is filtered out, and be seeded to autotrophy culture medium solid slope, in low temperature and irradiance It is saved in incubator；
Step 3: the High Density Cultivation of microalgae cell
Step 2 inclined-plane culture algae is seeded to bioreactor to cultivate, in the Heterotrophic culture base of addition organic carbon source High Density Cultivation, until cell log growth period, cell density reaches 106-1010A/ml or more；
The high-density culturing condition: organic carbon source concentration of glucose 0.01-200 g.L-1, it is passed through the ventilatory capacity 150- of air 250L/h, using 5-40 μm of ol.m-2s-1Solar radiation, pH value control is in 5-9, hunting speed 100-150rpm, when total culture Between between 100-150h；
Step 4: the harvest of microalgae cell
The microalgae cell of step 3 High Density Cultivation is centrifuged with the revolving speed of 800-4000rpm, the microalgae cell of harvest It is harvested with after aseptic water washing 2-3 times；
Step 5: the purification of culture and waste water of the microalgae cell in sauce waste water
The microalgae cell that step 4 is harvested is transferred to progress illumination shaken cultivation in the sauce waste water of different extension rates, absorbs Each nutriment in sauce waste water reduces pollutant concentration in sauce waste water, purifies sauce waste water；
The condition of culture: sauce waste water distilled water or tap water according to 5 times of volume ratio dilution, 2 times of dilution or do not dilute, The initial inoculum of microalgae cell is 106-108A/ml, 20-45 DEG C of temperature, hunting speed 100-150rpm, using 10-200 μ mol.m-2s-1Solar radiation, total incubation time is between 1-15d；
Step 6: the biomass recycling use of microalgae
Recycling step 5 purify sauce waste water after microalgae, to microalgae carry out biomass recycling use, be used as feed, bait or Bioenergy raw material；
The autotrophy culture medium prescription are as follows: K2HPO4·3H2O0.04g/L, MgSO4·7H2O 0.075g/L, CaCl2·2H2O 0.036g/L, citric acid 0.006g/L, ferric citrate 0.006g/L, EDTA0.001g/L, NaNO31.5g/L Na2CO30.02g/L, A5 liquid microelement 1.5ml/L；Wherein A5 liquid microelement forms: H3BO32.86g/L MnCl2· 4H2O1.81g/L, ZnSO4·7H2O0.222g/L, NaMoO4·2H2O0.39g/L, CuSO4·5H2O 0.079g/L and CoCl2·6H2O 0.05g/L；
The Heterotrophic culture based formulas: it is 1-20 g.L that organic carbon source to initial reduction sugar concentration is added in autotrophy culture medium-1。
2. a kind of method of microalgae processing sauce waste water according to claim 1, it is characterised in that: the microalgae includes But it is not limited to Chlorella, barrel mast Trentepohlia, spirulina, diatom, diamond shape algae, splits pot algae, Dunaliella, scenedesmus, Nannochloropsis oculata, clothing Trentepohlia, flat algae or empty ball Trentepohlia.
3. a kind of method of microalgae processing sauce waste water according to claim 1, it is characterised in that: the organic carbon packet Include but be not limited to monosaccharide, disaccharides, polysaccharide, organic acid or alcohols.
CN201811631894.9A 2018-12-29 2018-12-29 A kind of method of microalgae processing sauce waste water Pending CN109626584A (en)

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Cited By (7)

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CN110627213A (en) * 2019-06-10 2019-12-31 华南理工大学 Method for efficiently treating high-ammonia-nitrogen wastewater by microalgae photo-fermentation method
CN111808754A (en) * 2020-07-01 2020-10-23 武汉理工大学 Mining area soil microalgae and separation and purification method and application thereof
CN112707510A (en) * 2020-12-08 2021-04-27 河海大学 Method for coupling furfural wastewater treatment and microalgae culture
CN112811597A (en) * 2021-01-15 2021-05-18 南昌航空大学 Microalgae culture and wastewater domestication integrated photobioreactor and use method thereof
CN113717855A (en) * 2021-09-08 2021-11-30 南昌大学 Gluten production wastewater resource utilization method
CN114105309A (en) * 2021-12-01 2022-03-01 北京林业大学 Method for purifying garbage leachate by using chlorella
CN114669585A (en) * 2022-03-14 2022-06-28 昆明理工大学 Method for weathered soil and resource utilization of silicate material

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CN112707510B (en) * 2020-12-08 2022-07-12 河海大学 Method for coupling furfural wastewater treatment and microalgae culture
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