CN109576151A - Magnetic method nucleic acid automatic extracting device - Google Patents
Magnetic method nucleic acid automatic extracting device Download PDFInfo
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- CN109576151A CN109576151A CN201910090538.9A CN201910090538A CN109576151A CN 109576151 A CN109576151 A CN 109576151A CN 201910090538 A CN201910090538 A CN 201910090538A CN 109576151 A CN109576151 A CN 109576151A
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- 230000005291 magnetic effect Effects 0.000 title claims abstract description 104
- 238000000034 method Methods 0.000 title claims abstract description 32
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 31
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 31
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 31
- 230000001681 protective effect Effects 0.000 claims abstract description 55
- 238000003756 stirring Methods 0.000 claims abstract description 33
- 238000000926 separation method Methods 0.000 claims description 44
- 238000010438 heat treatment Methods 0.000 claims description 18
- 238000009413 insulation Methods 0.000 claims description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims description 9
- 238000003825 pressing Methods 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 5
- 238000005336 cracking Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 8
- 238000000605 extraction Methods 0.000 description 7
- 239000011324 bead Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000010425 asbestos Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007257 malfunction Effects 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229910052895 riebeckite Inorganic materials 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of nucleic acid-extracting apparatus, especially magnetic method nucleic acid automatic extracting devices, including protective housing；Protective door is hinged on the front of protective housing；Touch screen controller is fixed on the front of protective housing；The inside of positioning seat fixed protection case, positioning seat are equipped with locating slot；Magnetic bar is fixed on magnetic frame；Vibration stirring device is connect with magnetic frame；Bar magnet set is actively set on magnetic bar；Bar magnet set is mounted on casing rack；Casing separator is mounted on vibration stirring device, and casing rack is housed on casing separator；Horizontal mobile device is fixed on protective housing bottom, and vibration stirring device is fixed on horizontal mobile device；Vibration stirring device is of reciprocating vibration up and down for driving magnetic bar and bar magnet to cover；Vibration stirring device and casing separator are connect with touch screen controller.The apparatus structure is simple and convenient to operate, occupies little space, is compact-sized, using safe, work efficiency is high, can customize cracking, eluting temperature.
Description
Technical field
The present invention relates to a kind of nucleic acid-extracting apparatus, especially magnetic method nucleic acid automatic extracting device.
Background technique
Paramagnetic particle method nucleic acid extraction is method more mature at present, for extracting the samples such as animal vegetable tissue, blood, body fluid
DNA and RNA.Magnetic bead is super magnetic-particle, there is certain adsorption capacity to nucleic acid, and protein and other impurities is not inhaled
It is attached, stay in solution.Under magnetic fields, magnetic bead is separated with liquid, magnetic bead is recycled, to obtain magnetic bead-mixtures of nucleic acids, finally
Nucleic acid can be obtained through elution.
Structure is complicated for existing instrument for extracting nucleic acid, and due to needing to separate bar magnet with the magnetic bead in lysate,
To realize agitating function simultaneously, therefore structure is complicated for current paramagnetic particle method nucleic acid extraction agitating device, occupied space is larger, and
And bar magnet set dismounting trouble.
Summary of the invention
To solve the above problems, the present invention provides a kind of magnetic method nucleic acid automatic extracting device that structure is simple and convenient to operate,
The specific technical proposal is:
Magnetic method nucleic acid automatic extracting device, including protective housing；Protective door, the protective door are transparent doors, the protective door
It is hinged on the front of protective housing；Touch screen controller, the touch screen controller are fixed on the front of protective housing；Positioning seat, institute
The inside of positioning seat fixed protection case is stated, and is located at the front side of protective housing, the positioning seat is equipped with locating slot, the locating slot
For placing deep-well plates；Magnetic bar, the magnetic bar are equipped with multiple；Magnetic frame, the magnetic bar is fixed on magnetic frame, and is pressed
Column setting；Vibration stirring device, the vibration stirring device are connect with magnetic frame, and are located at the rear of positioning seat；Bar magnet set, institute
Bar magnet set is stated to be actively set on magnetic bar；Casing rack, the bar magnet set are mounted on casing rack；Casing separator, the set
Pipe separator is mounted on vibration stirring device, and casing rack, described sleeve pipe separator are housed on described sleeve pipe separator
It is separated for covering bar magnet with magnetic bar；And horizontal mobile device, the horizontal mobile device is fixed on protective housing bottom, described
Vibration stirring device is fixed on horizontal mobile device；The magnetic bar and bar magnet set are respectively positioned on the top of positioning seat, and are located at
In protective housing；The vibration stirring device is of reciprocating vibration up and down for driving magnetic bar and bar magnet to cover；The vibration stirring device
It is connect with touch screen controller with casing separator.
By using above-mentioned technical proposal, touch screen controller is easy to use.
Bar magnet set without cleaning magnetic bar, while can be avoided cross contamination, use for being isolated solution with magnetic bar
It is convenient, also facilitate the separation of magnetic bead and magnetic bar.
Bar magnet set and magnetic bar are installed on vibration stirring device, carried out to realize that bar magnet set is synchronous with magnetic bar
Upper and lower is of reciprocating vibration, and is fixed on vibration by casing separator separation bar magnet set and magnetic bar, casing separator and stirs
It is compact-sized for mixing on device, is occupied little space.
Preferably, the magnetic bar is equipped with two column, and each column is equipped with eight magnetic bars, and each column magnetic bar is and in the deep-well plates of hole
Each column deep hole it is corresponding, at a distance of five column deep holes between two column magnetic bars.
By using above-mentioned technical proposal, deep-well plates are 96 hole deep-well plates, and two column magnetic bars can be realized 16 parts every time
The extraction of sample, working efficiency are higher.
Preferably, the vibration stirring device includes vibrating grid；Vibrating motor, the vibrating motor are fixed on vibrating grid
Top；Ball screw is vibrated, one end of the vibration ball screw is connect with vibrating motor, and vibration ball screw is vertically arranged；
Feed screw nut is vibrated, the vibration feed screw nut is movably arranged on vibration ball screw；Vibration bearing seat, the vibration bearing
Seat is fixed on vibrating grid, and the vibration ball screw is rotatably installed on vibration bearing seat；Vibrate linear guide, the vibration
Linear guide is fixed on vibrating grid, and parallel with vibration ball screw；Sliding block is vibrated, the vibration sliding block is slidably mounted on vibration
In dynamic linear guide；Vibrating seat, the vibrating seat are fixed on vibration sliding block, and connect with vibration feed screw nut, the magnetic force
Frame is fixed on vibrating seat.
By using above-mentioned technical proposal, vibrating motor is servo motor, and servo motor can be accurately controlled angle of rotation
Degree, to realize accurate position control.Vibrating motor drives vibration ball screw positive and negative rotation by positive and negative rotation, to realize vibration
Dynamic feed screw nut moves back and forth up and down, and can be realized the effect of oscillation when carrying out high revolving speed, small distance reciprocating movement, passes through magnetic
The vibration realizing solution of power stick mixes.
The high transmission accuracy of ball screw, long service life can effectively ensure that the distance of vibration, to guarantee to mix effect
Fruit.Ball screw and linear guide are used cooperatively stable structure, occupy little space.
Preferably, described sleeve pipe separator includes separation sliding block, and the separation sliding block is slidably mounted on vibration straight line and leads
On rail, and it is located at the top of vibration sliding block；Separation block, the separation block is fixed on separation sliding block, and separation block and casing rack
Connection；Feed screw nut is separated, the separation feed screw nut is fixed on separation block；Separate ball screw, the separation ball wire
Bar is movably arranged on separation feed screw nut；Motor is separated, the separation motor is fixed on vibrating seat, and is separated motor and divided
One end connection from ball screw.
By using above-mentioned technical proposal, separation block and vibrating seat share a linear guide and simplify structure, make structure
Simply, while volume is reduced.
It preferably, further include plate, the bar magnet set is fixed on plate, and is corresponded with magnetic bar, the plate
It is inserted in fixing groove, the fixing groove is arranged on casing rack, and fixing groove is through slot；The plate is equipped with positioning convex platform,
The positioning convex platform is stuck in the end of fixing groove.
By using above-mentioned technical proposal, plate is equipped with positioning convex platform, and positioning convex platform is stuck in the end of fixing groove, positions
Boss corresponds bar magnet set with magnetic bar, the positioning for facilitating bar magnet to cover.Since bar magnet set is consumables, needed after each use
It replaces, the quick-replaceable of bar magnet set is realized by plate, and facilitate installation, it is very easy to use.
Preferably, the horizontal mobile device includes transverse bottom plate, and the transverse bottom plate is fixed on the bottom of protective housing；It is horizontal
To linear guide, the transverse direction linear guide is fixed in transverse bottom plate；Transverse slider, the transverse slider are slidably mounted on cross
To in linear guide；Lateral bearing block, the transverse direction bearing block are fixed in transverse bottom plate；Lateral seat, the lateral seat are fixed
On transverse slider；Cross lead screw nut, the cross lead screw nut are connect with lateral seat；Transverse ball lead screw, the transverse direction
Ball screw is rotatably installed on lateral bearing block, and parallel with lateral linear guide, and the cross lead screw nut is slidably installed
On transverse ball lead screw；And cross motor, the cross motor are fixed in transverse bottom plate, and one with transverse ball lead screw
End connection.
It preferably, further include pressing device, the pressing device includes fixed link, and the fixed link is mounted on positioning seat
On；Gag lever post, the gag lever post are mounted on the top of fixed link；Compact heap, the compact heap are movably arranged on gag lever post；And
Holddown spring, the holddown spring are cylindrical spring, and the holddown spring is inserted in gag lever post, and one end of the holddown spring is pressed in
On gag lever post, the other end is pressed on compact heap；The compact heap is pressed in the top of deep-well plates.
By using above-mentioned technical proposal, pressing device can be such that deep-well plates are pressed in locating slot, effectively prevent vibrating
The bounce of whipping process medium-length hole plate and displacement, to guarantee that is automatically extracted goes on smoothly.
It preferably, further include heating device, the heating device includes heated seats, and the bottom of the heated seats is equipped with heating
Slot；Thermal insulation board, the thermal insulation board are equipped with heat dam, and the heated seats are inserted in heat dam；Electric boiling plate, the electric boiling plate
It is fixed in heating tank；And thermocouple, the thermocouple are fixed in heated seats；The bottom of the locating slot of the positioning seat is equipped with
Bottoming hole, the heated seats are fixed in bottoming hole, and thermal insulation board is between bottoming hole and heated seats.
By using above-mentioned technical proposal, the experimental temperature that heating device can be such that the solution in deep-well plates keeps constant,
It can be gone on smoothly to guarantee to extract, improve success rate.Asbestos production or the production of other heat-barrier materials, energy can be used in thermal insulation board
It enough prevents from heating entire positioning seat.
It preferably, further include axial flow blower, the axial flow blower is fixed on the exhaust vent at the back side of protective housing, and axis stream
Blower is located at the inside of protective housing, and axial flow blower blows to the outside of protective housing；And HEPA filter, the HEPA filter installation
On the exhaust vent at the protective housing back side, and seal exhaust vent.
By using above-mentioned technical proposal, the exhaust gas of protective housing is discharged axial flow blower in time, avoids polluting, and lead to
It crosses HEPA filter to be filtered, prevents pollution environment.HEPA filter is periodically replaced.
It preferably, further include reagent chip, the reagent chip is RFID label tag, and the reagent chip is used for choice experiment
Program；Reader, the reader is fixed on protection cabinet, and is connect with touch screen controller.
By using above-mentioned technical proposal, the method for extracting nucleic acid of every kind of animal or plant is all different, and is extracted every time equal
It needs to be programmed according to guiding book, it is very inconvenient, it is also easy to malfunction, therefore program is arranged according to different animal or plants
It gets well and exists in touch screen controller, RFID label tag is put on reader and carries out for a RFID label tag by every kind of program
It reads, then reader sends information to touch screen controller, and touch screen controller chooses corresponding program automatically, can have
Effect avoids procedure selection mistake or misprogrammed.
Compared with prior art the invention has the following advantages:
Magnetic method nucleic acid automatic extracting device magnetic method nucleic acid automatic extracting device structure provided by the invention is simple, operation side
Just, occupy little space, is compact-sized, using safe, work efficiency is high, can customize cracking, eluting temperature.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of magnetic method nucleic acid automatic extracting device；
Fig. 2 is that magnetic method nucleic acid automatic extracting device hides the structural schematic diagram after protective housing；
Fig. 3 is the assembling structure schematic diagram of vibration stirring device, horizontal mobile device and casing separator；
Fig. 4 is the side view of the assembly of vibration stirring device, horizontal mobile device and casing separator；
Fig. 5 is the structural schematic diagram of vibration stirring device；
Fig. 6 is the structural schematic diagram of vibrating seat；
Fig. 7 is the structural schematic diagram of plate；
Fig. 8 is the structural representation of casing rack；
Fig. 9 is the assembling structure schematic diagram of positioning seat；
Figure 10 is the configuration schematic diagram of heating device.
Specific embodiment
Now in conjunction with attached drawing, the invention will be further described.
Embodiment one
As shown in Figures 1 to 10, magnetic method nucleic acid automatic extracting device, including protective housing 120；Protective door 110, protective door
110 be transparent doors, and protective door 110 is hinged on the front of protective housing 120；Touch screen controller 130, touch screen controller 130 are solid
It is scheduled on the front of protective housing 120；Positioning seat 42, the inside of 42 fixed protection case 120 of positioning seat, and before protective housing 120
Side, positioning seat 42 are equipped with locating slot 421, and locating slot 421 is for placing deep-well plates 190；Magnetic bar 222, magnetic bar 222 are equipped with
It is multiple；Magnetic frame 221, magnetic bar 222 are fixed on magnetic frame 221, and by column setting；Vibration stirring device, Vibratory Mixing dress
It sets and is connect with magnetic frame 221, and be located at the rear of positioning seat 42；Bar magnet set 232, bar magnet set 232 are actively set on magnetic bar 222
On；Casing rack 231, bar magnet set 232 are mounted on casing rack 231；Casing separator, casing separator are mounted on vibration and stir
It mixes on device, casing rack 231 is housed on casing separator, casing separator is used to divide bar magnet set 232 with magnetic bar 222
From；Magnetic bar 222 and bar magnet set 232 are respectively positioned on the top of positioning seat 42, and are located in protective housing 120；And horizontal mobile device,
Horizontal mobile device is fixed on the bottom of protective housing 120, and vibration stirring device is fixed on horizontal mobile device；Vibratory Mixing dress
It sets and is up and down reciprocatingly vibrated for driving magnetic bar 222 and bar magnet to cover 232；Vibration stirring device, horizontal mobile device and casing point
It is connect with touch screen controller 130 from device.
The top of protective housing 120 is equipped with ultraviolet lamp, and ultraviolet lamp is for sterilizing.Protective door 110 can block ultraviolet light.Such as figure
Shown in 1, the front of protective housing 120 is also equipped with infrared sensor 140, infrared sensor 140 detect people close to when make touch screen
Controller 130 is lighted automatically.Touch screen controller 130 is easy to use.
Bar magnet set 232 and magnetic bar 222 are installed on vibration stirring device, to realize bar magnet set 232 and magnetic force
It is of reciprocating vibration above and below the synchronous progress of stick 222, and bar magnet set 232 and magnetic bar 222 are separated by casing separator, casing divides
It is compact-sized for being fixed on vibration stirring device from device, is occupied little space.
Magnetic bar 222 be equipped with two column, each column be equipped with eight magnetic bars 222, each column magnetic bar 222 with hole deep-well plates 190
On each column deep hole it is corresponding, at a distance of five column deep holes between two column magnetic bars 222.
Deep-well plates 190 are 96 hole deep-well plates, and two column magnetic bars 222 can be realized the extraction of 16 parts of sample, work every time
Efficiency is higher.
Two groups of magnetic bars 222 are symmetrical arranged, once can be realized 32 after being symmetrical arranged two locating slots 421 on positioning seat 42
The extraction of part sample.
Positioning seat 42 is mounted on fixed bottom plate 41, and fixed bottom plate 41 is fixed on the bottom of protective housing 120.
As shown in Figures 2 to 5, vibration stirring device includes vibrating grid 211；Vibrating motor 212, vibrating motor 212 are fixed
At the top of vibrating grid 211；Ball screw 216 is vibrated, one end of vibration ball screw 216 connect with vibrating motor 212, vibrates
Ball screw 216 is vertically arranged；Feed screw nut 217 is vibrated, vibration feed screw nut 217 is movably arranged on vibration ball screw 216
On；Vibration bearing seat 218, vibration bearing seat 218 are fixed on vibrating grid 211, and vibration ball screw 216 is rotatably installed in vibration
On bearing block 218；Vibrate linear guide 213, vibration linear guide 213 be fixed on vibrating grid 211, and with vibration ball screw
216 is parallel；Sliding block 214 is vibrated, vibration sliding block 214 is slidably mounted in vibration linear guide 213；Vibrating seat 215, vibrating seat
215 are fixed on vibration sliding block 214, and connect with vibration feed screw nut 217, and magnetic frame 221 is fixed on vibrating seat 215.
Vibrating motor 212 is servo motor, and servo motor can be accurately controlled rotational angle, to realize accurate position
Set control.Vibrating motor 212 drives vibration 216 positive and negative rotation of ball screw by positive and negative rotation, to realize vibration feed screw nut 217
It moves back and forth up and down, can be realized the effect of oscillation when carrying out high revolving speed, small distance reciprocating movement, pass through magnetic bar 222
Vibration realizing solution mixes.
The high transmission accuracy of ball screw, long service life can effectively ensure that the distance of vibration, to guarantee to mix effect
Fruit.
Ball screw and linear guide are used cooperatively stable structure, occupy little space.
As shown in fig. 6, vibrating seat 215 is equipped with nut groove 2151, flange groove 2152 and fixation hole 2153, flange groove 2152
Intersecting with nut groove 2151, fixation hole 2153 is communicated with flange groove 2152, and vibration feed screw nut 217 is inserted in nut groove 2151,
The flange of vibration feed screw nut 217 is inserted in flange groove 2152, and screw passes through fixation hole 2153 and vibrates the method for feed screw nut 217
Orchid connection.Vibrating seat 215 is compact-sized, and can effectively fix vibration feed screw nut 217, effectively reduces the volume of part.
As shown in Figures 2 to 5, casing separator includes separation sliding block 244, and separation sliding block 244 is slidably mounted on vibration
In linear guide 213, and it is located at the top of vibration sliding block 214；Separation block 245, separation block 245 are fixed on separation sliding block 244,
And separation block 245 is connect with casing rack 231；Feed screw nut 243 is separated, separation feed screw nut 243 is fixed on separation block 245；
Ball screw 242 is separated, separation ball screw 242 is movably arranged on separation feed screw nut 243；Separate motor 241, separation electricity
Machine 241 is fixed on vibrating seat 215, and is separated motor 241 and connected with the one end for separating ball screw 242.
After stirring, vibrating motor 212 drives vibrating seat 215 to rise by vibration ball screw 216, bar magnet set
232 and magnetic bar 222 be away from deep-well plates, take deep-well plates away, separation motor 241 drives separation roller screw rod 242 to rotate, separation
Roller screw rod 242 drives separation feed screw nut 243, and separation feed screw nut 243 drives separation block 245 to decline, and separation block 245 drives
Bar magnet set 232 is detached from magnetic bar 222, then pulls up plate 233.Separation block 245 and vibrating seat 215 share a linear guide letter
Structure is changed, has kept structure simple, while reducing volume.
As shown in Figure 7 and Figure 8, further include plate 233, bar magnet set 232 is fixed on plate 233, and with magnetic bar 222 1
One is corresponding, and plate 233 is inserted in fixing groove 2311, and fixing groove 2311 is convex, and fixing groove 2311 is through slot, fixing groove 2311
It is arranged on casing rack 231.The fixing groove 2311 of convex being capable of effective fixing insertion 233 and bar magnet set 232.
Plate 233 is equipped with positioning convex platform 234, and positioning convex platform 234 is stuck in the end of fixing groove 2311, positioning convex platform 234
Correspond bar magnet set 232 with magnetic bar 222.
It since bar magnet set 232 is consumables, is needed replacing after each use, when replacement pulls up plate 233, changes new insert
Plate 233.The quick-replaceable of bar magnet set 232 is realized by plate 233, and facilitates installation, it is very easy to use.
As shown in Figures 2 to 4, horizontal mobile device includes transverse bottom plate 318, and transverse bottom plate 318 is fixed on protective housing 120
Bottom；Lateral linear guide 313, lateral linear guide 313 are fixed in transverse bottom plate 318；Transverse slider 312 is laterally sliding
Block 312 is slidably mounted in lateral linear guide 313；Lateral bearing block 318, lateral bearing block 318 are fixed on transverse bottom plate 318
On；Lateral seat 311, lateral seat 311 are fixed on transverse slider 312；Cross lead screw nut 314, cross lead screw nut 314 and cross
It is connected to seat 311；Transverse ball lead screw 315, transverse ball lead screw 315 are rotatably installed on lateral bearing block 318, and with transverse direction
Linear guide 313 is parallel, and cross lead screw nut 314 is slidably mounted on transverse ball lead screw 315；Cross motor 317, it is laterally electric
Machine 317 is fixed in transverse bottom plate 318, and is connect with one end of transverse ball lead screw 315.
Horizontal mobile device is moved easily magnetic bar 222, needed for completing the reaction hole location experiment of each column, i.e., to realize
All deep holes are stirred or are inserted into corresponding hole.Since nucleic acid extraction has defined method and steps,
Not all deep hole is respectively arranged with solution, therefore can meet the requirements to each deep-well plates two column magnetic bars 222 of setting.Two column
Magnetic bar 222 is symmetrical arranged, and respectively corresponds six column deep holes for the two column magnetic bar 222 of deep-well plates in 90 holes.It can simplify in this way
Structure reduces load, to realize the miniaturization of structure, and easy to use.
As shown in figure 9, further including pressing device, pressing device includes fixed link 43, and fixed link 43 is mounted on positioning seat 42
On；Gag lever post 46, gag lever post 46 are mounted on the top of fixed link 43；Compact heap 44, compact heap 44 are movably arranged on gag lever post 46
On；And holddown spring 45, holddown spring 45 are cylindrical spring, holddown spring 45 is inserted in gag lever post 46, one end of holddown spring 45
It is pressed on gag lever post 46, the other end is pressed on compact heap 44；Compact heap 44 is pressed in the top of deep-well plates 190.
Pressing device can be such that deep-well plates 190 are pressed in locating slot 421, effectively prevent deep-well plates during Vibratory Mixing
190 bounces and displacement, to guarantee that is automatically extracted goes on smoothly.By 44 pull-up of compact heap and rotates 180 degree and make compact heap 44
It is pressed in deep-well plates 190.
It as shown in Figure 10, further include heating device, heating device includes heated seats 51, and the bottom of heated seats 51 is equipped with heating
Slot 511；Thermal insulation board 52, thermal insulation board 52 are equipped with heat dam 521, and heated seats 51 are inserted in heat dam 521；Electric boiling plate 53, electricity
Heating plate 53 is fixed in heating tank 511；And thermocouple, thermocouple are fixed in heated seats 51；The locating slot 421 of positioning seat 42
Bottom be equipped with bottoming hole, heated seats 51 are fixed in bottoming hole, and thermal insulation board 52 is between bottoming hole and heated seats 51.
The cultivation temperature that heating device can be such that the solution in deep-well plates 190 keeps constant, to guarantee that extraction can be suitable
Benefit carries out, and improves success rate, as a result stable, reproducible.Asbestos production or the production of other heat-barrier materials can be used in thermal insulation board 52,
It can prevent from heating entire positioning seat 42.
As shown in Fig. 2, further including axial flow blower 160, axial flow blower 160 is fixed on the exhaust vent at the back side of protective housing 120
On, and axial flow blower 160 is located at the inside of protective housing 120, axial flow blower 160 blows to the outside of protective housing 120；And HEPA filtering
Device 150, HEPA filter 150 are mounted on the exhaust vent at 120 back side of protective housing, and seal exhaust vent.Axial flow blower 160 will be prevented
The exhaust gas of protecting box 120 is discharged in time, avoids polluting, and is filtered by HEPA filter 150, prevents pollution environment.
HEPA filter 150 is periodically replaced.
Embodiment two
It on the basis of the above embodiment 1, further include reagent chip, reagent chip is RFID label tag, and reagent chip is used for
Choice experiment program；Reader, reader are fixed on 120 body of protective housing, and connect with touch screen controller 130.
By using above-mentioned technical proposal, the method for extracting nucleic acid of every kind of animal or plant is all different, and is extracted every time equal
It needs to be programmed according to guiding book, it is very inconvenient, it is also easy to malfunction, therefore program is arranged according to different animal or plants
It gets well and exists in touch screen controller 130, it is enterprising that every kind of program for a RFID label tag, by RFID label tag is put into reader
Row is read, and then reader sends information to touch screen controller 130, and touch screen controller 130 is chosen corresponding automatically
Program can effectively avoid procedure selection mistake or misprogrammed, reduce programming time, improve working efficiency.
The built-in nine kinds of programs of touch screen controller 130, are provided with nine RFID label tags.
Since the operator of most of biology laboratories is the trainee without permanent working experience, in face of complicated numerous
When trivial programming, it is very easy to error.When in addition extracting the nucleic acid of different material, agents useful for same or magnetic bead are in the anti-of each step
Between seasonable, mixing time, stirring frequency, temperature control etc. is there is also difference, when any one of experimenter link control is bad,
That is expection is just not achieved in the purity of laboratory sample.Purity is not available not high sample.
RFID chip built in this equipment has been burnt on equipment touch screen controller 130 programming of common experimental,
Need to only swipe the card can identifying system corresponding software, provide more specialized experiment flow for client, raising sample purity.Simultaneously
Shortening personnel's programming time promotes working efficiency.
Claims (10)
1. magnetic method nucleic acid automatic extracting device, which is characterized in that including
Protective housing (120)；
Protective door (110), the protective door (110) are transparent doors, and the protective door (110) is being hinged on protective housing (120) just
Face；
Touch screen controller (130), the touch screen controller (130) are fixed on the front of protective housing (120)；
Positioning seat (42), the inside of positioning seat (42) the fixed protection case (120), and it is located at the front side of protective housing (120), institute
Positioning seat (42) are stated equipped with locating slot (421), the locating slot (421) is for placing deep-well plates (190)；
Magnetic bar (222), the magnetic bar (222) are equipped with multiple；
Magnetic frame (221), the magnetic bar (222) are fixed on magnetic frame (221), and by column setting；
Vibration stirring device, the vibration stirring device are connect with magnetic frame (221), and are located at the rear of positioning seat (42)；
Bar magnet set (232), the bar magnet set (232) are actively set on magnetic bar (222)；
Casing rack (231), the bar magnet set (232) are mounted on casing rack (231)；
Casing separator, described sleeve pipe separator are mounted on vibration stirring device, are equipped on described sleeve pipe separator
Casing rack (231), described sleeve pipe separator is for separating bar magnet set (232) with magnetic bar (222)；And
Horizontal mobile device, the horizontal mobile device are fixed on the bottom of protective housing (120), and the vibration stirring device is fixed
On horizontal mobile device；
The magnetic bar (222) and bar magnet set (232) are respectively positioned on the top of positioning seat (42), and are located in protective housing (120)；
The vibration stirring device is for driving magnetic bar (222) and bar magnet set (232) up and down reciprocatingly to vibrate；
The vibration stirring device, horizontal mobile device and casing separator are connect with touch screen controller (130).
2. magnetic method nucleic acid automatic extracting device according to claim 1, which is characterized in that the magnetic bar (222) is equipped with
Two column, each column be equipped with eight magnetic bars (222), each column magnetic bar (222) with each column deep hole phase in 96 hole deep-well plates (190)
It is corresponding, at a distance of five column deep holes between two column magnetic bars (222).
3. magnetic method nucleic acid automatic extracting device according to claim 1, which is characterized in that the vibration stirring device includes
Vibrating grid (211)；
Vibrating motor (212), the vibrating motor (212) are fixed on the top of vibrating grid (211)；
It vibrates ball screw (216), one end of vibration ball screw (216) is connect with vibrating motor (212), vibrates ball
Screw rod (216) is vertically arranged；
It vibrates feed screw nut (217), the vibration feed screw nut (217) is movably arranged in vibration ball screw (216)；
Vibration bearing seat (218), the vibration bearing seat (218) are fixed on vibrating grid (211), the vibration ball screw
(216) it is rotatably installed on vibration bearing seat (218)；
Vibrate linear guide (213), the vibration linear guide (213) is fixed on vibrating grid (211), and with vibrate ball wire
Bar (216) is parallel；
It vibrates sliding block (214), the vibration sliding block (214) is slidably mounted in vibration linear guide (213)；
Vibrating seat (215), the vibrating seat (215) are fixed in vibration sliding block (214), and even with vibration feed screw nut (217)
It connects, the magnetic frame (221) is fixed on vibrating seat (215).
4. magnetic method nucleic acid automatic extracting device according to claim 3, which is characterized in that described sleeve pipe separator includes
It separates sliding block (244), the separation sliding block (244) is slidably mounted in vibration linear guide (213), and is located at vibration and slides
The top of block (214)；
Separation block (245), the separation block (245) is fixed in separation sliding block (244), and separation block (245) and casing rack
(231) it connects；
It separates feed screw nut (243), the separation feed screw nut (243) is fixed on separation block (245)；
It separates ball screw (242), the separation ball screw (242) is movably arranged in separation feed screw nut (243)；
Separate motor (241), the separation motor (241) is fixed on vibrating seat (215), and separation motor (241) with separate
One end of ball screw (242) connects.
5. magnetic method nucleic acid automatic extracting device according to claim 2, which is characterized in that
It further include plate (233), the bar magnet set (232) is fixed on plate (233), and corresponds with magnetic bar (222),
The plate (233) is inserted in fixing groove (2311), and the fixing groove (2311) is arranged on casing rack (231)；
The plate 233 is equipped with positioning convex platform (234), and the positioning convex platform (234) is stuck in the end of fixing groove (2311).
6. magnetic method nucleic acid automatic extracting device according to claim 2, which is characterized in that the horizontal mobile device includes
Transverse bottom plate (318), the transverse bottom plate (318) are fixed on the bottom of protective housing (120)；
Lateral linear guide (313), the transverse direction linear guide (313) are fixed on transverse bottom plate (318)；
Transverse slider (312), the transverse slider (312) are slidably mounted on lateral linear guide (313)；
Lateral bearing block (318), the transverse direction bearing block (318) are fixed on transverse bottom plate (318)；
Lateral seat (311), the lateral seat (311) are fixed on transverse slider (312)；
Cross lead screw nut (314), the cross lead screw nut (314) connect with lateral seat (311)；
Transverse ball lead screw (315), the transverse ball lead screw (315) are rotatably installed on lateral bearing block (318), and with cross
In parallel to linear guide (313), the cross lead screw nut (314) is slidably mounted on transverse ball lead screw (315)；And
Cross motor (317), the cross motor (317) is fixed on transverse bottom plate (318), and and transverse ball lead screw
(315) one end connection.
7. magnetic method nucleic acid automatic extracting device according to claim 1, which is characterized in that it further include pressing device, it is described
Pressing device includes
Fixed link (43), the fixed link (43) are mounted on positioning seat (42)；
Gag lever post (46), the gag lever post (46) are mounted on the top of fixed link (43)；
Compact heap (44), the compact heap (44) are movably arranged on gag lever post (46)；And
Holddown spring (45), the holddown spring (45) are cylindrical spring, and the holddown spring (45) is inserted in gag lever post (46), institute
The one end for stating holddown spring (45) is pressed on gag lever post (46), and the other end is pressed on compact heap (44)；
The compact heap (44) is pressed in the top of deep-well plates (190).
8. magnetic method nucleic acid automatic extracting device according to claim 1, which is characterized in that it further include heating device, it is described
Heating device includes
The bottom of heated seats (51), the heated seats (51) is equipped with heating tank (511)；
Thermal insulation board (52), the thermal insulation board (52) are equipped with heat dam (521), and the heated seats (51) are inserted in heat dam (521)
In；
Electric boiling plate (53), the electric boiling plate (53) are fixed in heating tank (511)；And
Thermocouple, the thermocouple are fixed on heated seats (51)；
The bottom of the locating slot (421) of the positioning seat (42) is equipped with bottoming hole, and the heated seats (51) are fixed in bottoming hole,
And thermal insulation board (52) is located between bottoming hole and heated seats (51).
9. magnetic method nucleic acid automatic extracting device according to claim 1, which is characterized in that further include
Axial flow blower (160), on the exhaust vent at the back side that the axial flow blower (160) is fixed on protective housing (120), and axis stream wind
Machine (160) is located at the inside of protective housing (120), and axial flow blower (160) blows to the outside of protective housing (120)；And
HEPA filter (150), the HEPA filter (150) are mounted on the exhaust vent at protective housing (120) back side, and seal
Exhaust vent.
10. magnetic method nucleic acid automatic extracting device according to claim 1, which is characterized in that further include
Reagent chip, the reagent chip are RFID label tag, and the reagent chip is used for choice experiment program；And
Reader, the reader are fixed on protective housing (120) body, and connect with touch screen controller (130).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910090538.9A CN109576151A (en) | 2019-01-30 | 2019-01-30 | Magnetic method nucleic acid automatic extracting device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910090538.9A CN109576151A (en) | 2019-01-30 | 2019-01-30 | Magnetic method nucleic acid automatic extracting device |
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ID=65918171
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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