CN109540829A - A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole - Google Patents
A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole Download PDFInfo
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- CN109540829A CN109540829A CN201811521077.8A CN201811521077A CN109540829A CN 109540829 A CN109540829 A CN 109540829A CN 201811521077 A CN201811521077 A CN 201811521077A CN 109540829 A CN109540829 A CN 109540829A
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- 238000000034 method Methods 0.000 title claims abstract description 46
- 208000011231 Crohn disease Diseases 0.000 title claims abstract description 31
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 238000004566 IR spectroscopy Methods 0.000 title claims abstract description 16
- 239000005357 flat glass Substances 0.000 claims abstract description 25
- 239000012188 paraffin wax Substances 0.000 claims abstract description 23
- 230000008569 process Effects 0.000 claims abstract description 22
- 238000000862 absorption spectrum Methods 0.000 claims abstract description 21
- 238000001704 evaporation Methods 0.000 claims abstract description 21
- 229910052751 metal Inorganic materials 0.000 claims abstract description 21
- 239000002184 metal Substances 0.000 claims abstract description 21
- 239000011248 coating agent Substances 0.000 claims abstract description 20
- 238000000576 coating method Methods 0.000 claims abstract description 20
- 230000008020 evaporation Effects 0.000 claims abstract description 18
- 238000012360 testing method Methods 0.000 claims abstract description 18
- 239000011521 glass Substances 0.000 claims abstract description 12
- 238000007747 plating Methods 0.000 claims abstract description 12
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 6
- 238000005253 cladding Methods 0.000 claims description 5
- 238000007740 vapor deposition Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 238000004018 waxing Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 20
- 238000013461 design Methods 0.000 abstract description 2
- 238000005457 optimization Methods 0.000 abstract 1
- 230000009102 absorption Effects 0.000 description 19
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 7
- 229910052709 silver Inorganic materials 0.000 description 7
- 239000004332 silver Substances 0.000 description 7
- 201000008267 intestinal tuberculosis Diseases 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000002329 infrared spectrum Methods 0.000 description 4
- 230000003746 surface roughness Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 150000001408 amides Chemical class 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000002425 crystallisation Methods 0.000 description 2
- 230000008025 crystallization Effects 0.000 description 2
- 239000012024 dehydrating agents Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000006911 nucleation Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010018691 Granuloma Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229910001622 calcium bromide Inorganic materials 0.000 description 1
- WGEFECGEFUFIQW-UHFFFAOYSA-L calcium dibromide Chemical compound [Ca+2].[Br-].[Br-] WGEFECGEFUFIQW-UHFFFAOYSA-L 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000001142 circular dichroism spectrum Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 238000013100 final test Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
- G01N2021/3572—Preparation of samples, e.g. salt matrices
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses the method for making sample that a kind of infrared spectroscopy detection technique is used for Crohn disease antidiastole, use the sheet glass with the coat of metal as the specimen holder of the carrier of fishing piece process and infrared absorption spectrum test, the paraffin section of tissue sample carries out infrared absorption spectrum test after completing dewaxing process；Infrared spectroscopy detection technique of the present invention is used for the method for making sample of Crohn disease antidiastole, the design of the glass sample frame coat of metal can reduce the cost of specimen holder, the coat of metal is to infrared light substantially without absorption simultaneously, by adjusting the roughness of process reform coating surface during using evaporation plating, facilitate adherency of the histotomy on coating；The optimization of dewaxing technique reduces paraffin residual and interferes infrared absorption spectrum.
Description
Technical field
The present invention relates to fields of biomedicine more particularly to a kind of infrared spectroscopy detection technique to examine for Crohn disease identification
Disconnected method for making sample.
Background technique
Crohn disease (Crohn ' s disease, CD) is a kind of gastrointestinal tract chronic non infectious that the cause of disease is not still fully aware of
Property granulomatous diseases recall rate is low, defficulty in diagnosing.Intestinal tuberculosis (intestinal tuberculosis, TB) is tuberculosis
The chronic specific infection of enteron aisle caused by mycobacteria.The antidiastole of Crohn disease and intestinal tuberculosis generally requires to combine clinical table
Existing, scope, histology and iconography carry out comprehensive analysis.In clinical practice, the identification ten of intestinal tuberculosis and Crohn disease (CD)
Divide important.Clinic there is no effective means identification intestinal tuberculosis and Crohn disease, misdiagnosis rate to reach 50%-70% at present, for clinic
Doctor's diagnosis and treatment bring great difficulty.
Fourier transformation decaying total reflection middle infrared spectrum detection technique has detection speed fast, and Detection accuracy is high, right
The harmless equal many merits of test sample, have been widely used in each detection field.It is that illumination is mapped to substance table that it, which analyzes foundation,
Face causes the variation of material molecule fundamental vibration, and different material is in middle infrared band (4000-400cm-1) there is different absorptions,
By the information of variation, qualitative and quantitative analysis can be carried out to substance.
In stomach intestinal tissue in addition to water, mainly comprising organic compounds such as protein, lipid, carbohydrates.These substances are all
There is very strong infrared active.As protein is main in the characteristic absorption of middle infrared band are as follows: amidocarbonylation C=O stretching vibration is again
Referred to as amide I band (1685-1630cm-1) and R-CONH2NH in molecule2Angle vibration is known as amide II band (1640-1600cm-1).Different diseases can cause these component ratios in tissue to change, in infrared absorption can also generate variation.To sum up institute
It states, Crohn disease can be identified by mid infrared absorption spectrum.
Slice research need to be carried out to the detection of tissue, exemplary steps are that organization material, fixer is taken to impregnate, washing, take off
Water, waxdip, slice, fishing piece, roasting piece, finally carry out infrared absorption spectrum test.Fishing piece behaviour is generally carried out using glass sample frame
Make, while also as final test sample frame.Do not use the dedicated infrared test eyeglass of calcium bromide etc that can generate stronger,
And the price of dedicated infrared test eyeglass is more expensive.It needs to do many slice tests during diagnosis, also relates to a large amount of samples
The cleaning problem of frame is very big for the cost consumption of specimen holder in this way.Paraffin simultaneously in paraffin section is infrared in
2956cm-1, 2916cm-1, 2848cm-1, 1461cm-1, 719cm-1Tissue spectrum signal can be generated on five wave bands strong dry
It disturbs.
Summary of the invention
It is an object of the invention to overcome the deficiencies of existing technologies, a kind of infrared spectroscopy detection technique is provided for Crow
The method for making sample of grace disease antidiastole, the technical solution are achieved in that
A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole, and method for making sample specific steps are such as
Under:
1, it after the tissue sample for taking proper site, is put into fixer and handles so that tissue sample toughness increases, typically admittedly
Determine the formalin solution that liquid is 10%-18%.
2, it takes out before the tissue sample after fixing is dehydrated and is cleaned using pure water, the tissue sample sequence after cleaning is put
The dehydrating agent of various concentration carries out dehydration, and most common dehydrating agent is ethyl alcohol.Present dehydration can also be using special
Dewaterer is completed.
3, the tissue sample after the completion of being dehydrated carries out waxdip, slice, fishing piece, dries piece process.
4, using the sheet glass with the coat of metal as the carrier of fishing piece process and the sample of infrared absorption spectrum test
Frame.
5, after the completion of drying piece process, tissue sample paraffin section carries out dewaxing process, finally carries out infrared absorption spectrum survey
Examination.
Preferably, the paraffin section of tissue sample carries out infrared absorption spectrum test after completing dewaxing process.Described group
The paraffin section de-waxing process of tissue samples is naturally dry after dewaxing 20-30 hours in dimethylbenzene pure solvent.Processing group in this way
Knit not easy to fall off, dewaxing effect is also obvious.
Preferably, the coat of metal is silvering.
Preferably, the coat of metal of the sheet glass is added using the technique of evaporation plating.
Preferably, during the evaporation metal cladding of the sheet glass, final coating is adjusted by changing technological parameter
Roughness.For the examination of infrared spectrum of histotomy, the roughness of coating will not influence greatly test result very much.Fish out piece mistake
There is certain roughness can facilitate adherency of the histotomy on coating for Cheng Zhong, coating.If directlying adopt with certain coarse
When the infrared test specialized lens of degree fish out piece, the roughness of eyeglass can reduce the transparency of entire eyeglass, and be unfavorable for fishing out piece mistake
Journey.The method of the roughness of final coating is improved to increase sheet glass temperature during evaporation rate and raising vapor deposition.Although evaporation
The evaporation source category of plating is different, but with the evaporation rate of evaporation source constantly increase, nucleation rate increases, crystal grain coarsening rate increases
Add, the surface roughness of coating is bigger.Sheet glass temperature during being deposited is improved, metal vapors molecule is easy to transport on the glass sheet
It moves or by substrate re-evaporation, the crystallization of bulky grain easy to form improves the surface roughness of coating.
Preferably, the sheet glass is evaporated during metal cladding, using the grinding tool piece with different shape plated hole
It is bonded in glass sheet surface, finally obtains metal-plated point of different shapes.It needs to do many histotomies during diagnosis
Infrared absorption spectrum, the sheet glass with metal-plated point of different shapes can be directly as the label of histotomy.
TB and CD are in 1000-1400cm-1The difference difference of the infrared absorption spectrum of range is obvious, and predominantly CD exists
1234cm-1With 1074cm-1The difference of phosphodiester bond peak.
Substrate of glass is in 785cm-1, 990cm-1, 1145cm-1Wave band has very strong absorption peak, to the spectrum knot of TB and CD
Fruit has very big interference.Nearly all metal is all seldom to the absorption of infrared ray, infrared absorption light of the metallic silver in TB and CD
Spectrum detection range almost interferes test.
In order to overcome the influence of paraffin background, we carry out dewaxing treatment, dewaxing and the slice not dewaxed to paraffin section
Mid infrared absorption spectrum figure, paraffin is in 1461cm-1, 2849cm-1, 2922cm-1Wave band has very strong absorption peak.Paraffin is cut
For the characteristic absorption of piece with the change curve of dewaxing time, dewaxing 6/12/18/24 hour has stronger absorption to infrared spectroscopy,
The absorption of paraffin obviously weakens after dewaxing 30 hours, and as the extension of dewaxing time tends towards stability substantially.
Infrared spectroscopy detection technique provided by the invention is used for the method for making sample of Crohn disease antidiastole, in order to realize pair
The fast and accurately antidiastole of Crohn disease and intestinal tuberculosis, sample making course improvement have the advantages that
1, the design of glass sample frame metal cladding can reduce the cost of specimen holder, while the coat of metal is to infrared light base
This, will not distinguishing disturbance process without absorption.
2, label can easily be added during glass sample frame metal cladding, as sample serial number.
3, by adjusting the roughness of process reform coating surface during plating using evaporation, histotomy is facilitated to plate
Adherency on layer.
4, single step dewaxing technique process can simply be polluted except infrared absorption of the deparaffnize for diagnosis of Crohn disease.
Detailed description of the invention
Invention is further described in detail with reference to the accompanying drawings and detailed description；
Fig. 1 is that TB of the present invention is compareed with the infrared absorption spectrum of CD.
Fig. 2 is the infrared absorpting light spectra of sheet glass of the present invention.
Fig. 3 is the histotomy infrared absorpting light spectra that the present invention dewaxes and do not dewax.
Fig. 4 is change curve of the tissue paraffin section de infrared absorption spectrum of the present invention with the dewaxing time.
Fig. 5 is the sheet glass that the present invention has different shape silver plating point.
Specific embodiment
A kind of disclosed infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole, sample preparation
Specific step is as follows for method:
1, it after the tissue sample for taking proper site, is put into fixer and handles so that tissue sample toughness increases, typically admittedly
Determine the formalin solution that liquid is 10%-18%.
2, it takes out before the tissue sample after fixing is dehydrated and is cleaned using pure water, the tissue sample sequence after cleaning is put
The ethyl alcohol of various concentration carries out dehydration.
3, the tissue sample after the completion of being dehydrated carries out waxdip, slice, fishing piece, dries piece process.
4, using the sheet glass 1 with the coat of metal as the carrier of fishing piece process and the sample of infrared absorption spectrum test
Frame.
5, after the completion of drying piece process, tissue sample paraffin section carries out dewaxing process, finally carries out infrared absorption spectrum survey
Examination.
The paraffin section of tissue sample carries out infrared absorption spectrum test after completing dewaxing process.The tissue sample
Paraffin section de-waxing process is naturally dry after dewaxing 30 hours in dimethylbenzene pure solvent.Processing tissue in this way is not easy to take off
It falls, dewaxing effect is also obvious.
The coat of metal is silvering.
The silvering of the sheet glass 1 is added using the technique of evaporation plating.
During the evaporation silver coating of the sheet glass 1, the roughness of final coating is adjusted by changing technological parameter.It is right
In the examination of infrared spectrum of histotomy, the roughness of coating will not influence greatly test result very much.During fishing out piece, coating
Adherency of the histotomy on coating can be facilitated with certain roughness.If directlying adopt the infrared survey with certain roughness
When trying specialized lens fishing piece, the roughness of eyeglass can reduce the transparency of entire eyeglass, and be unfavorable for fishing out piece process.Resistance-type is steamed
Plating instrument improves the roughness of final coating by 1 temperature of sheet glass during increase evaporation rate and raising vapor deposition, wherein vapor deposition is electric
Stream chooses 200A, and 1 temperature of sheet glass is 150 degrees Celsius during vapor deposition.Although the evaporation source category for evaporating plating is different, with
The evaporation rate of evaporation source constantly increase, nucleation rate increases, crystal grain coarsening rate increases, and the surface roughness of coating is bigger.
Sheet glass temperature during being deposited is improved, silver-colored vapour molecule is easy to move on sheet glass 1 or by 1 re-evaporation of sheet glass, hold
The crystallization for easily forming bulky grain, improves the surface roughness of coating.
The sheet glass 1 is evaporated during silver coating, is bonded in glass using the grinding tool piece with different shape plated hole
Glass piece surface finally obtains silver plating point 2 of different shapes.Need to do many histotomy infrared absorption light during diagnosis
Spectrum, as shown in figure 5, the sheet glass 1 with silver plating point 2 of different shapes can be directly as the label of histotomy.
As shown in Figure 1, TB and CD are in 1000-1400cm-1The difference difference of the infrared absorption spectrum of range is obvious, predominantly
CD is in 1234cm-1With 1074cm-1The difference of phosphodiester bond peak.
As shown in Fig. 2, substrate of glass is in 785cm-1, 990cm-1, 1145cm-1Wave band has very strong absorption peak, to TB with
The spectral results of CD have very big interference.Nearly all metal is all seldom to the absorption of infrared ray, and metallic silver is TB's and CD
Infrared absorption spectrum detection range almost interferes test.
In order to overcome the influence of paraffin background, we to paraffin section carry out dewaxing treatment, as shown in figure 3, dewaxing and not
The mid infrared absorption spectrum figure of the slice of dewaxing, paraffin is in 1461cm-1, 2849cm-1, 2922cm-1Wave band has very strong absorption
Peak.As shown in figure 4, change curve of the characteristic absorption of paraffin section with the dewaxing time, dewaxes 6/12/18/24 hour to infrared
Spectrum has stronger absorption, and the absorption of paraffin obviously weakens after dewaxing 30 hours, and with the extension base of dewaxing time
Originally it tends towards stability.
Embodiment is only one embodiment of the present of invention, for those of ordinary skill in the art, is not paying creation
Property it is laborious under the premise of, to the modification that carries out of the present invention, also within protection scope of the present invention.
Claims (6)
1. the method for making sample that a kind of infrared spectroscopy detection technique is used for Crohn disease antidiastole, it is characterised in that: using having
The sheet glass of the coat of metal is cut as the carrier of fishing piece process and the specimen holder of infrared absorption spectrum test, the paraffin of tissue sample
Piece carries out infrared absorption spectrum test after completing dewaxing process.
2. infrared spectroscopy detection technique according to claim 1 is used for the method for making sample of Crohn disease antidiastole, special
Sign is: the paraffin section de-waxing process of the tissue sample is after dewaxing 20-30 hours in dimethylbenzene pure solvent, to dry in the air naturally
It is dry.
3. infrared spectroscopy detection technique according to claim 1 is used for the method for making sample of Crohn disease antidiastole, special
Sign is: the coat of metal is silvering.
4. the sample preparation side that infrared spectroscopy detection technique according to claim 1 or 2 or 3 is used for Crohn disease antidiastole
Method, it is characterised in that: the coat of metal of the sheet glass is added using the technique of evaporation plating.
5. infrared spectroscopy detection technique according to claim 4 is used for the method for making sample of Crohn disease antidiastole, special
Sign is: during the evaporation plating of the sheet glass metal layer, by increasing evaporation rate and sheet glass temperature during raising vapor deposition
It spends to increase the roughness of final coating.
6. infrared spectroscopy detection technique according to claim 4 is used for the method for making sample of Crohn disease antidiastole, special
Sign is: the sheet glass is evaporated during metal cladding, is bonded in glass using the grinding tool piece with different shape plated hole
Glass piece surface obtains metal-plated point of different shapes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811521077.8A CN109540829A (en) | 2018-12-12 | 2018-12-12 | A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811521077.8A CN109540829A (en) | 2018-12-12 | 2018-12-12 | A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole |
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| CN109540829A true CN109540829A (en) | 2019-03-29 |
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| VINAY K. KATUKURI 等: "Detection of colonic inflammation with Fourier transform infrared spectroscopy using a flexible silver halide fiber", 《BIOMED OPT EXPRESS》 * |
| 张子杰: "《新编临床常见肿瘤诊断与处理》", 30 April 2014, 西安：西安交通大学出版社 * |
| 郑娜: "FTIR及mapping技术探测陈旧性及急性心肌梗死的分子学差异", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
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