CN109456876A - Bioreactor with agitating function - Google Patents
Bioreactor with agitating function Download PDFInfo
- Publication number
- CN109456876A CN109456876A CN201811360220.XA CN201811360220A CN109456876A CN 109456876 A CN109456876 A CN 109456876A CN 201811360220 A CN201811360220 A CN 201811360220A CN 109456876 A CN109456876 A CN 109456876A
- Authority
- CN
- China
- Prior art keywords
- rack body
- stirring motor
- reaction kettle
- fangs
- agitating function
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses a kind of bioreactors with agitating function, comprising: reaction kettle and host;Reaction kettle is installed to host;Reaction kettle includes: the tank body for accommodating culture solution;Tank body includes: cover and fangs;Fangs is formed with the culture chamber for accommodating culture solution;The bottom of culture chamber is formed with multiple stirring bars;The opening of cover covering culture chamber;Host includes: body, peristaltic pump, controller and stirring motor;Peristaltic pump is used to be reaction kettle feed flow;Controller is for controlling peristaltic pump;Stirring motor is for driving fangs to rotate relative to cover;Peristaltic pump, controller and stirring motor are installed to body.Bioreactor with agitating function, is not provided with independent agitating paddle, and sealing effect is not easy to reveal well.
Description
Technical field
The present invention relates to a kind of bioreactors.
Background technique
Independent agitating paddle is usually arranged in traditional bioreactor, and agitating paddle is extend into reaction kettle and is stirred
It mixes.This mode makes, and the transmission shaft of agitating paddle needs the tank skin across reaction kettle higher to the sealing requirements of sealing structure,
It is at high cost and be easy to produce leakage.
Summary of the invention
To solve the deficiencies in the prior art, it the present invention provides a kind of bioreactor with agitating function, is not provided with
Independent agitating paddle is not susceptible to reveal.
In order to achieve the above objectives, the present invention adopts the following technical scheme that:
A kind of bioreactor with agitating function, comprising: reaction kettle and host;Reaction kettle is installed to host;Reaction
Kettle includes: the tank body for accommodating culture solution;Tank body includes: cover and fangs;Fangs is formed with the culture chamber for accommodating culture solution;
The bottom of culture chamber is formed with multiple stirring bars;The opening of cover covering culture chamber;Host includes: body, peristaltic pump, controller
And stirring motor;Peristaltic pump is used to be reaction kettle feed flow;Controller is for controlling peristaltic pump;Stirring motor is for driving fangs phase
Cover is rotated;Peristaltic pump, controller and stirring motor are installed to body.
Further, stirring motor drives fangs relative to cover reciprocating rotation.
Further, fangs is equipped with driven gear;Stirring motor is connected with driving gear;Driving gear drives passive tooth
Wheel rotates so that fangs rotates.
Further, the periphery of the bottom of fangs is formed with driven gear.
Further, host further include: oxygen sensor, for detecting the oxygen in reaction kettle;Temperature sensor is used for
Detect the temperature in reaction kettle;With pH sensor, for detecting the pH value in reaction kettle;Oxygen sensor, temperature sensor and
PH sensor is detachably connected to tank body.
Further, oxygen sensor, temperature sensor and pH sensor are detachably connected to cover.
Further, body includes: the first Rack Body and the second Rack Body;Peristaltic pump and controller are installed to the first rack
Body;Stirring motor is installed to the second Rack Body;Tank body is installed to the second Rack Body;Second Rack Body is detachably installed to the first machine
Frame body.
Further, the first Rack Body is equipped with: the first electric connection terminal;Second Rack Body is equipped with: the second electric connection terminal;
Second electric connection terminal is electrically connected to stirring motor;The power supply electricity that first electric connection terminal is electrically connected to as stirring motor power supply
Source;When second Rack Body is connect with the first Rack Body, the first electric connection terminal and the second electric connection terminal composition be electrically connected thus
Enable power supply for stirring motor power supply.
Further, the second Rack Body is formed to the side far from the first Rack Body far from one end of the first Rack Body and is protruded
Arcuate structure.
Further, the upper surface of one end far from the first Rack Body of the second Rack Body is constituted semicircle.
The invention has the beneficial effects that having the bioreactor of agitating function, it is not provided with independent agitating paddle, is used
The mode of rotation tank body 11 is stirred, and is not susceptible to reveal.
The body of bioreactor can dismount to pack and transport.Second Rack Body is connect with the first Rack Body
When, the first electric connection terminal is electrically connected to enable power supply for stirring motor power supply with the second electric connection terminal composition.
When the second Rack Body and the first Rack Body connect, the electrical connection of electrical equipment can be realized automatically, it is convenient to use.
Detailed description of the invention
Fig. 1 is a kind of perspective view of bioreactor of the invention;
Fig. 2 is the perspective view at another visual angle of the bioreactor in Fig. 1;
Fig. 3 is the top view of the bioreactor in Fig. 1;
Fig. 4 is the front view of the bioreactor in Fig. 1;
Fig. 5 is the first Rack Body of the bioreactor in Fig. 4 and the schematic diagram that the second Rack Body is separated from each other;
Fig. 6 is the schematic diagram of the part-structure of the bioreactor in Fig. 1.
Bioreactor 100, reaction kettle 10, tank body 11, cover 111, fangs 112, stirring bar 1111, driven gear
1112, host 20, body 21, the first Rack Body 21a, the first electric connection terminal 211a, the second Rack Body 21b, second is electrically connected
Terminal 211b, peristaltic pump 22, controller 23, stirring motor 24, electric machine support 24a, driving gear 241, touch control operation display screen
25, air filter 26.
Specific embodiment
Specific introduce is made to the present invention below in conjunction with the drawings and specific embodiments.
As shown in Figures 1 to 6, a kind of bioreactor 100, comprising: reaction kettle 10 and host 20.Reaction kettle 10 is installed extremely
Host 20.Reaction kettle 10 includes: tank body 11.Tank body 11 is for accommodating culture solution.It can be by culture solution when carrying out culture solution culture
It is delivered in tank body 11 and carries out biological culture.Tank body 11 includes: cover 111 and fangs 112.Fangs 112 is formed with receiving culture
The culture chamber of liquid.The bottom of culture chamber is formed with multiple stirring bars 1111.The opening of the covering culture chamber of cover 111.112 energy of fangs
Enough to move relative to cover 111, stirring bar 1111 plays stirring action and is stirred to the culture solution in tank body 11, avoids training
Nutrient solution precipitating, enables culture solution to come into full contact with so as to sufficiently react.
Host 20 includes: body 21, peristaltic pump 22, controller 23 and stirring motor 24.Peristaltic pump 22 is used to be reaction kettle
10 feed flows.It is reacted specifically, culture solution is delivered in tank body 11 by peristaltic pump 22.Controller 23 is for controlling peristaltic pump
22.Controller 23 instructs control peristaltic pump 22 to convey culture solution according to the input of user.Stirring motor 24 is for driving fangs 112
It is rotated relative to cover 111.Specifically, stirring motor 24 drives fangs 112 relative to 111 reciprocating rotation of cover to tank body 11
Interior culture solution is stirred.Host 20 further includes electric machine support 24a.Stirring motor 24 is fixed to electric machine support 24a.As one
Kind specific embodiment, peristaltic pump 22, controller 23, stirring motor 24 and the installation of tank body 11 to body 21.
As a kind of specific embodiment, fangs 112 is equipped with driven gear 1112. specifically, the bottom of fangs 112
The periphery in portion is formed with driven gear 1112.Stirring motor 24 is connected with driving gear 241.Stirring motor drives driving gear
241 rotations, driven gear 1112 is driven to rotate for driving gear 241 so that fangs 112 rotates.Principal and subordinate's gear 241 is fixed to actively
Gear shaft.The motor shaft and driving gear shaft of shaft coupling connection stirring motor 24.
As a preferred embodiment, host 20 further include: touch control operation display screen 25.Touch control operation display screen 25
Installation is to body 21, so that user inputs operation instruction or show the relevant information about bioreactor 100.
As a preferred embodiment, host 20 further include: oxygen sensor, temperature sensor and pH sensor.
Oxygen sensor is used to detect the oxygen in reaction kettle 10, and the oxygen information that will test is sent to touch control operation display screen
25.Temperature sensor is used to detect the temperature in reaction kettle 10, and the temperature information that will test is sent to touch control operation and shows
Screen 25.PH sensor is used to detect the pH value in reaction kettle 10, and the pH value information that will test is sent to touch control operation and shows
Screen 25.
As a kind of specific embodiment, oxygen sensor, temperature sensor and pH sensor are detachably connected to
Tank body 11 can be disassembled installation to new tank body 11 and continue to detect when in order to replace tank body 11.Specifically,
Oxygen sensor, temperature sensor and pH sensor are detachably connected to cover 111, and the rotation of fangs 112 will not be to sensing
The installation of device has an impact.
As a preferred embodiment, host 20 further include: air filter 26 and gas-guide tube.Air filter 26
For being filtered to the gas being input in reaction kettle 10, the substance of the obstruction reaction in gas is avoided to enter in reaction kettle 10
Influence reaction rate.It is for air guide tube to be directed into reaction kettle 10 in by the filtered gas of air filter 26.
As a kind of specific embodiment, gas-guide tube is detachably connected to tank body 11, will when in order to changing tank body 11
Gas-guide tube disassembles installation to new tank body 11, continues the filtered gas of air filter 26 being directed into new reaction
In kettle 10.
As a preferred embodiment, host 20 further include: catheter.Peristaltic pump 22 is led liquid by catheter
Enter to reaction kettle 10.As a kind of specific embodiment, catheter is detachably connected to tank body 11, in order to changing tank body
Catheter is disassembled into installation to new tank body 11 when 11, continues for the filtered liquid of air filter 26 to be directed into newly
Reaction kettle 10 in.
Body 21 includes: the first Rack Body 21a and the second Rack Body 21b.Peristaltic pump 22 and controller 23 are installed to first
Rack Body 21a.Stirring motor 24 is installed to the second Rack Body 21b.Tank body 11 is installed to the second Rack Body 21b.Second Rack Body
21b is detachably installed to the first Rack Body 21a.Therefore, bioreactor 100 can be referred to as assembly type bioreactor again
100.Specifically, the second Rack Body 21b detachably install to the first Rack Body 21a can in transporting biological reactor 100,
Second Rack Body 21b is disassembled from the first Rack Body 21a.It is convenient for the transport of bioreactor 100 in this way.
The bottom of first Rack Body 21a and the second Rack Body 21b are equipped with support wheel.User can push the second Rack Body
21b moves the assembling for realizing the first Rack Body 21a and the second Rack Body 21b relative to the first Rack Body 21a.
As a preferred embodiment, the first Rack Body 21a is equipped with: the first electric connection terminal 211a.Second rack
Body 21b is equipped with: the second electric connection terminal 211b.Second electric connection terminal 211b is electrically connected to stirring motor 24.First electrical connection
Terminal 211a is electrically connected to the power supply powered for stirring motor 24.Second Rack Body 21b is connect with the first Rack Body 21a
When, the first electric connection terminal 211a is electrically connected to enable power supply for stirring electricity with the second electric connection terminal 211b composition
Machine 24 is powered.
Specifically, the first electric connection terminal 211a is arranged in the first Rack Body 21a, in the second Rack Body 21b setting the
Two electric connection terminal 211b, when the second Rack Body 21b is connect with the first Rack Body 21a, the first electric connection terminal 211a and
Two electric connection terminal 211b constitute electrical connection to enable power supply for the power supply of stirring motor 24.Setting is avoided in machine in this way
A plurality of cable electrical connection power supply and stirring motor 24 are set outside body 21, to avoid being located at the cable outside body 21
Line loss badly causes installation problems.Meanwhile it being connect by the second Rack Body 21b with the first Rack Body 21a and can realize that first is electrically connected
The electric connection of connecting terminal 211a and the second electric connection terminal 211b, structure is simple, easy for installation.
As a preferred embodiment, the second electric connection terminal 211b inserting relative to the first electric connection terminal 211a
Pull out installation direction of the direction perpendicular to tank body 11 relative to the second Rack Body 21b.
As a preferred embodiment, tank body 11 relative to the second Rack Body 21b installation direction perpendicular to second
Installation direction of the Rack Body 21b relative to the first Rack Body 21a.
As a preferred embodiment, touch control operation display screen 25 is installed to the first Rack Body 21a.As a kind of excellent
The embodiment of choosing, detachably installation carries out more air filter 26 to the first Rack Body 21a air filter 26
It changes.
As a preferred embodiment, the second Rack Body 21b is formed far from one end of the first Rack Body 21a to separate
The arcuate structure of the side protrusion of first Rack Body 21a.
As a preferred embodiment, the upper surface of one end far from the first Rack Body 21a of the second Rack Body 21b
It constitutes semicircle.
Specifically, setting semicircle for the upper surface of one end far from the first Rack Body 21a of the second Rack Body 21b
Shape, second Rack Body 21b will not generate interference to user when convenient for the user to operate.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the above embodiments do not limit the invention in any form, all obtained by the way of equivalent substitution or equivalent transformation
Technical solution is fallen within the scope of protection of the present invention.
Claims (10)
1. a kind of bioreactor with agitating function characterized by comprising reaction kettle and host;The reaction kettle peace
It is filled to the host;The reaction kettle includes: the tank body for accommodating culture solution;The tank body includes: cover and fangs;It is described
Fangs is formed with the culture chamber for accommodating culture solution;The bottom of the culture chamber is formed with multiple stirring bars;The cover covers institute
State the opening of culture chamber;The host includes: body, peristaltic pump, controller and stirring motor;Peristaltic pump is used to be the reaction
Kettle feed flow;Controller is for controlling the peristaltic pump;Stirring motor is for driving the fangs to rotate relative to the cover;Institute
Peristaltic pump, the controller and the stirring motor is stated to install to the body.
2. the bioreactor according to claim 1 with agitating function, which is characterized in that
The stirring motor drives the fangs relative to the cover reciprocating rotation.
3. the bioreactor according to claim 2 with agitating function, which is characterized in that
The fangs is equipped with driven gear;The stirring motor is connected with driving gear;The driving gear drives the quilt
Moving gear rotates so that the fangs rotates.
4. the bioreactor according to claim 3 with agitating function, which is characterized in that
The periphery of the bottom of the fangs is formed with the driven gear.
5. the bioreactor according to claim 1 with agitating function, which is characterized in that
The host further include:
Oxygen sensor, for detecting the oxygen in the reaction kettle;
Temperature sensor, for detecting the temperature in the reaction kettle;With
PH sensor, for detecting the pH value in the reaction kettle;
The oxygen sensor, the temperature sensor and the pH sensor are detachably connected to the tank body.
6. the bioreactor according to claim 5 with agitating function, which is characterized in that
The oxygen sensor, the temperature sensor and the pH sensor are detachably connected to cover.
7. the bioreactor according to claim 1 with agitating function, which is characterized in that
The body includes: the first Rack Body and the second Rack Body;The peristaltic pump and the controller are installed to described first
Rack Body;The stirring motor is installed to second Rack Body;The tank body is installed to second Rack Body;Described second
Rack Body is detachably installed to first Rack Body.
8. the bioreactor according to claim 7 with agitating function, which is characterized in that
First Rack Body is equipped with: the first electric connection terminal;Second Rack Body is equipped with: the second electric connection terminal;It is described
Second electric connection terminal is electrically connected to the stirring motor;First electric connection terminal is electrically connected to as stirring motor confession
The power supply of electricity;When second Rack Body is connect with first Rack Body, first electric connection terminal and described the
Two electric connection terminals constitute electrical connection to enable power supply for stirring motor power supply.
9. the bioreactor according to claim 7 with agitating function, which is characterized in that
Second Rack Body, which is formed far from one end of first Rack Body to the side far from first Rack Body, to be protruded
Arcuate structure.
10. the bioreactor according to claim 9 with agitating function, which is characterized in that
The upper surface of one end far from first Rack Body of second Rack Body constitutes semicircle.
Priority Applications (1)
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CN201811360220.XA CN109456876A (en) | 2018-11-15 | 2018-11-15 | Bioreactor with agitating function |
Applications Claiming Priority (1)
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CN201811360220.XA CN109456876A (en) | 2018-11-15 | 2018-11-15 | Bioreactor with agitating function |
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CN201811360220.XA Pending CN109456876A (en) | 2018-11-15 | 2018-11-15 | Bioreactor with agitating function |
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US20180282770A1 (en) * | 2013-12-13 | 2018-10-04 | The Trustees Of Columbia University In The City Of New York | Methods and systems for converting volatile fatty acids to lipids |
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