CN109425573A - A kind of solution detection device and detection method - Google Patents
A kind of solution detection device and detection method Download PDFInfo
- Publication number
- CN109425573A CN109425573A CN201710770834.4A CN201710770834A CN109425573A CN 109425573 A CN109425573 A CN 109425573A CN 201710770834 A CN201710770834 A CN 201710770834A CN 109425573 A CN109425573 A CN 109425573A
- Authority
- CN
- China
- Prior art keywords
- test paper
- solution
- detection device
- detection
- groove
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/031—Multipass arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
Abstract
The invention discloses a kind of solution detection devices, and the detection method being adapted with the detection device.The detection device is used cooperatively with test tubes, the detection device is provided with the detection box that can be used in installing test tubes and chromatographic test paper and upper cover and rear cover for sealing, the detection box is internally provided with the mounting groove of contact pin and delivery hole, it realizes the effect detected in the environment of sealing to the solution in test tubes, eliminates the process extracted solution from test tubes using tools such as dropper, liquid-transfering guns and be moved to chromatographic test paper.The solution detection device and corresponding detection method that the present invention provides a kind of structures is simple, detection accuracy is high.
Description
Technical field
The present application is related to solution detection field more particularly to a kind of solution detection device and detection method.
Background technique
For many researchs, medical treatment and industrial application, it often may require that and solution detected, including egg
White, enzyme linked immunological etc. has volatility, has pollution, poisonous and hazardous reagent solution, with volatile solution detection process
In, it needs to prevent volatile materials from overflowing, falling or large area contacts with environment and generates Aerosols Evaporation etc. pair at room temperature
Environment leads to the problem of pollution.
For example, PCR product solution is one of solution with volatile materials.Polymerase chain reaction
(Polymerase Chain Reaction), the external primer of abbreviation PCR, also known as cell-free molecular cloning or specific DNA sequences
Enzymatic amplification technique is oriented, is a kind of method of external enzyme' s catalysis specific DNA fragment, because of tool in obtained PCR product solution
The ingredient for having aerosol etc. volatile, therefore volatile materials should be avoided to influence the accuracy detected as far as possible in the detection process.
In practical application, in order to characterize the state of solution, need to detect solution, usually using chromatographic test paper come
Solution is detected.
For example, for the detection of specific PCR product solution, in addition to because the reason of human operational error it is caused
Product overflows, and falls outside, i.e., can be because reaction mixture is the same as big as long as the test tubes equipped with PCR product solution to be detected are uncapped
Gas contacts and occurs the volatilization of aerosol, this volatilization is inevitable existing.The usual product of PCR after reaction is dense
Degree is very high, therefore reaction mixture is easy to pollute the environment in the case where uncapping, so that subsequent PCR detects hair
The probability of raw false positive greatly improves, and influences the accuracy of subsequent testing result, while contaminated clean environment is also very numerous
It is multiple.
After being expanded using conventional PCR reaction tube, if necessary to carry out end point determination to reaction mixture, usually will
PCR reaction mixture is extracted from routine PCR reaction pipe by tools such as dropper or liquid-transfering guns, is being added dropwise to chromatographic test paper
In detected.Detecting step is cumbersome in above-mentioned detection mode, and may be mixed into outside in the pipetting processes of PCR product
In impurity, the precision of testing result is influenced, meanwhile, it also can not effective solution for volatilization, the pollution problem of aerosol.
Likewise, for other are with volatile solution, because being examined caused by volatile materials in detection process
Result inaccuracy and environmental pollution are surveyed, is problem in urgent need to solve.Therefore it provides it is a kind of it is easy to operate, in laboratory ring
Border and site environment can be used and can be efficiently applied to the detection device of volatile products solution and detection method exists
Quickly it is of great significance in detection.
Summary of the invention
In order to overcome the problems, such as present in the relevant technologies, the present invention provides it is a kind of it is easy to operate, detection accuracy is high
A kind of solution detection device and detection method.
To achieve the above object, present invention employs following technical solutions:
A kind of solution detection device, the detection device 2 are used cooperatively with test tubes 1, and the test tubes 1 include pipe
Body 11 and lid 12, the lower end outer wall of the tube body 11 are the sealing films 13 of cladding setting, and the detection device 2 includes using
In detection box 21, the upper cover 22 of installing the test tubes 1, the upper cover 22 is pivoted on one end of the detection box 21
Bottom can be simultaneously fastened on the detection box 21, wherein
Be set as stepped in the detection box 21, including bottom plate 211, be arranged in 211 one end of bottom plate side wall 212 and
The top plate 213 at 212 top of side wall is set,
The installation for installing the test tubes 1 is offered on the top plate 213 one end top surface adjacent with upper cover 22
The edge of slot 214, the mounting groove 214 is connected with detection 21 edge of box；It is provided in installation trough floor 2141 for stabbing
The contact pin 2142 for breaking the sealing film 13 offers in the installation trough floor 2141 of 2142 side of contact pin for connecting
Lead to the delivery hole 2143 of the mounting groove 214 and chromatographic test paper 25；
The observation window of the state change for observing chromatographic test paper 25 is also provided on the top surface other end of the top plate 213
215, the test paper form 23 for sealing is installed on the observation window 215；
At the back side of the top plate 213, i.e., the examination of the described detection box 21 being internally provided with for installing chromatographic test paper 25
Paper locating part 216；
One end of the bottom plate 211 has been pivotally mounted upper cover 22, and the other end is offered for placing the chromatographic test paper 25
Opening 2111, and configured with for sealing rear cover 24, the rear cover 24 be detachably arranged in it is described opening 2111 on.
Preferably, be connected with detection 21 edge of the box installation trough floor 2141 of one end of the mounting groove 214 is provided with the
One positive stop strip 2144, the side wall of mounting groove 214 and the connection of top plate 213 are provided with second to 214 internal stretch of mounting groove
The gap of positive stop strip 2145, first positive stop strip 2144 and the second positive stop strip 2145 is formed for being inserted into inserting for test tubes
Mouth retention bead.
Preferably, 11 on both sides of the middle of tube body of the test tubes 1 is provided with the first retention bead 114,
It is offered and first limit on the side wall of the mounting groove 214 along the direction perpendicular to installation trough floor 2141
The limiting slot 2148 that protrusion 114 matches.
Preferably, the end of the tube body 11 of the test tubes 1 is provided with positioning column 115；
The end of the mounting groove 214 is provided with the groove mutually matched with positioning column 115, the side of the groove two sides
Be respectively arranged with limited post 2146 on wall, the installation of positioning column 115 in a groove and the side wall of 11 end of tube body with it is described
Limited post 2146 abuts against.
Preferably, one end that the contact pin 2142 is connected with installation trough floor 2141 is column structure, and the other end is cone
Shape structure is radially offered for guiding solution to be detected to flow to delivery hole 2143 on the outer surface of the contact pin 2142
Intake chute 2147.
Preferably, the chromatographic test paper 25 can be colloid gold test paper, and the colloid gold test paper includes the sample set gradually
Product pad 251, bonding pad 252, chromatography layer 253 and water-absorption material backing strap 254,
The test paper locating part 216 includes that first support 2161 at the back side both ends of top plate 213 is arranged in and is arranged in
Second support 2162 in portion,
First support 2161 and the second support 2162 are located along the same line, the sample pad of the colloid gold test paper
One end where 251 is mounted in the first support 2161 corresponding with 214 one end of mounting groove, where water-absorption material backing strap 254
One end is mounted in another first support 2161, and the bonding pad 252 is in contact with the second support 2162.
Preferably, the back side both ends of the top plate 213 are respectively arranged with position-limiting drum 2160, each position-limiting drum 2160
It is respectively set jagged on side wall and constitutes first support 2161, be provided on the both sides of the edge of the notch for card
Fill the second retention bead 2163 of the chromatographic test paper 25；
The initial makeup location for limiting the chromatographic test paper 25 is provided on the side wall of at least one position-limiting drum 2160
Third retention bead 2164.
Preferably, the upper cover 22 includes cover board 220, is provided with and the contact pin on the inner surface of the cover board 220
2142 it is mutually matched press pressure ring 222, be provided with non-slip texture 227 on outer surface；
It is additionally provided with the first mounting post 223 on the inner surface of the cover board 220, is gone back on the top plate 213 of the detection box 21
It is provided with the first mounting hole 2131, first mounting post 223, which can be embedded in, to be mounted in first mounting hole 2131.
Preferably, on the top plate 213 of the detection box 21 and the bottom plate 211 is connected the plate of one end with upper cover 22
Face both sides of the edge offer the first seal groove 217 respectively；
One end of the cover board 220 is pivoted on the detection box 21, and the other end can be overlapped on the top plate 213
On, it is provided with the side plate 221 that can be fastened on detection 21 two sides of box between both ends, the edge of the side plate 221 and takes
The end margin connect in the top plate 213 is separately provided for the first sealing strip 224 sealed, first sealing strip 224
It can fasten and be embedded in and be mounted in first seal groove 217.
Preferably, at least one positive stop strip 226, institute are provided on the side plate 221 that the upper cover 22 is connected with bottom plate 211
Stating positive stop strip 226 can abut against with the lid 12 for the test tubes 1 being mounted in mounting groove 214.
Preferably, the observation window 215 is the groove being arranged on top plate 213, including the window being arranged in groove base plate
Mouth 2151, is arranged in the seal groove 2153 at bottom portion of groove edge and is used for the words identification 2152 for characterizing testing result
Second mounting hole 2154 of test paper form 23 is installed；
The test paper form 23 is made of transparent plastics material, including observation piece 230, the inner surface of the observation piece 230
Both ends be provided with the second mounting post 231, and the inner surface edge for observing piece 230 is provided with for Sealed Observation Window 215
Second sealing strip 232；
Second mounting post 231 of the observation window 215 is mounted in second mounting hole 2154, and described second close
Strip of paper used for sealing 232, which can be embedded in, to be mounted in the seal groove 2153.
Preferably, the inner surface of the rear cover 24 is provided with multiple third mounting posts 241, the top plate of the detection box 21
Third mounting hole 219 is provided on 213 back side, the third mounting post 241, which can be embedded in, is mounted on the third mounting hole
In 219；
The both ends of 24 inner surface of rear cover are also respectively provided with fixed lobe 243, and the fixed lobe 243 can be with peace
Chromatographic test paper 25 in the detection box 21 abuts against；
The edge of the rear cover 24 is additionally provided with the third sealing strip 242 for sealing, the opening 2111 of the bottom plate 211
Edge be provided with third seal groove 2112, the third sealing strip 242, which can be embedded in, is mounted on the third seal groove 2112
It is interior.
Preferably, one end of the bottom plate 211 is provided with hemispherical protuberances or mounting hole, and one end of the upper cover 22 is set
The pivotal axis 228 matched with the hemispherical protuberances or mounting hole is set, the pivotal axis 228 is pivoted on the institute
State one end of bottom plate 211.
In addition, the present invention also provides a kind of solution detection method, the method includes,
By solution to be detected in test tubes 1,
One end that the test tubes 1 are provided with sealing film 13 is mounted in mounting groove 214；
Upper cover 22 and detection box 21 are fastened, press the test tubes by pressure ring 222 on 22 inner surface of upper cover
1, so that contact pin 2142 is poked sealing film 13, solution to be detected flows into the sample pad 251 of chromatographic test paper 25 by delivery hole 2143
On.
It is of the invention to provide a kind of solution detection device and corresponding detection method, the detection device can directly with dress
There are the test tubes of solution to be detected to be used cooperatively, is provided with the detection box that can be used in installing test tubes and chromatographic test paper
With the upper cover and rear cover for sealing, directly the internal test tubes equipped with solution to be detected can be inserted into detection device, button
Upper cover pressing, the mounting groove are internally provided with contact pin and delivery hole, pass through matching on upper interior surface by pressure ring and contact pin
The test tubes are poked in conjunction, and realization detects the solution in test tubes in the environment of sealing, are eliminated using drop
The tools such as pipe, liquid-transfering gun extract solution from test tube and are moved to the process of chromatographic test paper, and mode of operation is efficient and convenient.Meanwhile
The detection device assembled by detection box, upper cover and rear cover realizes closed environment, can be applied to volatile materials
The detection of solution effectively prevent the spilling of volatile materials, avoids Aerosol Pollution, makes subsequent experiment due to Aerosol Pollution
Cause the probability of testing result inaccuracy to substantially reduce, while avoiding the harm of operator and the pollution of environment.
Detailed description of the invention
It, below will be to attached drawing needed in the embodiment in order to illustrate more clearly of the technical solution of the present application
It is briefly described, it should be apparent that, for those of ordinary skills, before not making the creative labor property
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the overall schematic that one embodiment of the present of invention is equipped with test tubes；
Fig. 2 is the overall structure diagram of matched test tubes in one embodiment of the present of invention；
Fig. 3 is the internal cross section schematic diagram of the matched test tubes of one embodiment of the present of invention；
Fig. 4 is the detection device overall structure diagram of one embodiment of the present of invention；
Fig. 5 is the detection device the schematic diagram of the section structure of one embodiment of the present of invention；
Fig. 6 A is the planar structure schematic diagram of the detection device detection box top of one embodiment of the present of invention；
Fig. 6 B is the schematic perspective view of the detection device detection box top of one embodiment of the present of invention；
Fig. 7 is the detection device and test tubes scheme of installation of one embodiment of the present of invention；
Fig. 8 is the floor map of test tubes installation on detection means in one embodiment of the present of invention；
Fig. 9 is the scheme of installation that box and upper cover are detected in one embodiment of the present of invention；
Figure 10 A is the upper cover outer surface structure schematic diagram of one embodiment of the present of invention；
Figure 10 B is the upper interior surface structural schematic diagram of one embodiment of the present of invention；
Figure 11 is upper cover and detection box cooperation schematic diagram in one embodiment of the present of invention；
Figure 12 A is the planar structure schematic diagram in the detection cassette bottom face of the detection device of one embodiment of the present of invention；
Figure 12 B is the schematic perspective view in the detection cassette bottom face of the detection device of one embodiment of the present of invention；
Figure 13 is the structural schematic diagram of the chromatographic test paper of one embodiment of the present of invention；
Figure 14 is scheme of installation of the chromatographic test paper on detection box；
Figure 15 A is the test paper form bottom surface structure schematic diagram of the detection device of one embodiment of the present of invention；
Figure 15 B is the test paper form top surface structure schematic diagram of the detection device of one embodiment of the present of invention；
Figure 16 is scheme of installation of the test paper form on detection box in one embodiment of the present of invention；
Figure 17 is test paper form and observation window cooperation sealing schematic diagram in one embodiment of the present of invention；
Figure 18 is the cooperation schematic diagram that words identification and chromatographic test paper on box are detected in one embodiment of the present of invention；
Figure 19 is rear cover inner surface structure schematic diagram in one embodiment of the present of invention；
Figure 20 is the scheme of installation of rear cover and detection box in one embodiment of the present of invention；
Figure 21 is the cooperation sealing schematic diagram of rear cover and detection box in one embodiment of the present of invention；
Figure 22 be three in one embodiment of the present of invention at labyrinth seal structure schematic diagram.
In figure: 1- test tubes, 2- detection device, 11- tube body, 12- lid, 13- sealing film, 21- detect box, 22-
Upper cover, 23- test paper form, 24- lid, 25- chromatographic test paper, 111- infuse sample mouth, and 112- infuses sample channel, 113- sample cavity, 114-
First retention bead, 115- positioning column, 211- bottom plate, 212- side wall, 213- top plate, 214- mounting groove, 215- observation window,
216- test paper locating part, the first seal groove of 217-, 219- third mounting hole, 220- cover board, 221- side plate, 222- pressure ring, 223-
First mounting post, the first sealing strip of 224-, 225- shallow slot, 226- positive stop strip, 227- non-slip texture, 228- pivotal axis, 230- are seen
Piece is examined, the second mounting post of 231-, the second sealing strip of 232-, 241- third mounting post, 242- third sealing strip, 243- fixes convex
It rises, 251- sample pad, 252- bonding pad, 253- chromatographs layer, 254- water-absorption material backing strap, 2111- opening, the sealing of 2112- third
Slot, the first mounting hole of 2131-, 2141- installation trough floor, 2142- contact pin, 2143- delivery hole, the first positive stop strip of 2144-,
The second positive stop strip of 2145-, 2146- limited post, 2147- intake chute, 2148- limiting slot, 2151- window, 2152- words identification,
2153- seal groove, the second mounting hole of 2154-, 2160- position-limiting drum, 2161- first are supported, and 2162- second is supported, 2163- the
Two retention beads, 2164- third retention bead.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Whole description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts it is all its
His embodiment, shall fall within the protection scope of the present invention.
The embodiment of the present invention provides a kind of solution detection device, and the solution can be with volatility or without waving
The solution of hair property, the present embodiment with volatile PCR product solution mainly to be illustrated, and certainly, the present invention is also
It can be adapted for including that albumen, enzyme linked immunological etc. have volatility, have pollution, poisonous and hazardous reagent solution, specific embodiment
Adaptive change can be made according to the difference of solution and chromatographic test paper, do not limited excessively herein.
Referring to Fig. 1 to Fig. 3 the embodiment described, the detection device 2 is used cooperatively with test tubes 1, the detection examination
Pipe 1 includes tube body 11 and lid 12, and the lower end outer wall of the tube body 11 is the sealing film 13 of cladding setting.Wherein, the pipe
The one end corresponding with lid 12 of body 11 is provided with note sample mouth 111, and the other end is provided with sample cavity 113, the note sample mouth
111 are connected with sample cavity 113 by infusing sample channel 112.
Referring to fig. 4 to Fig. 8, the detection device 2 include for installing the detection box 21 of the test tubes 1, upper cover 22,
The upper cover 22 is pivoted on one end bottom of the detection box 21 and can be fastened on the detection box 21, wherein
The detection box 21 is set as stepped, including bottom plate 211, and the side wall 212 of 211 one end of bottom plate is arranged in and is arranged in side
The top plate 213 at 212 top of wall.
The installation for installing the test tubes 1 is offered on the top plate 213 one end top surface adjacent with upper cover 22
The edge of slot 214, the mounting groove 214 is connected with detection 21 edge of box；It is provided in installation trough floor 2141 for stabbing
The contact pin 2142 for breaking the sealing film 13 offers in the installation trough floor 2141 of 2142 side of contact pin for connecting
Lead to the delivery hole 2143 of the mounting groove 214 and chromatographic test paper 25.The contact pin 2142 pokes the sealing film of test tubes 1
After 13, PCR product solution to be detected is flowed out from position is punctured, and is flowed to along delivery hole 2143 and be mounted on test paper limit
It is detected on chromatographic test paper 25 on part 216, and obtains testing result.
Preferably, one end that the contact pin 2142 is connected with installation trough floor 2141 is cylindrical structure, and the other end is
Pyramidal structure, the radial direction on the outer surface of the contact pin 2142 along cylinder are offered for guiding solution to be detected to flow to drain
The intake chute 2147 in hole 2143, solution to be detected can easily pass through the intake chute after sealing film 13 is punctured
2147 flow into delivery hole 2143, and then flow on chromatographic test paper 25 and detected.
The observation window of the state change for observing chromatographic test paper 25 is also provided on the top surface other end of the top plate 213
215, the test paper form 23 for sealing is installed on the observation window 215.
At the back side of the top plate 213, i.e., the examination of the described detection box 21 being internally provided with for installing chromatographic test paper 25
Paper locating part 216；One end of the bottom plate 211 has been pivotally mounted upper cover 22, and the other end is offered for placing the chromatography examination
The opening 2111 of paper 25, and configured with the rear cover 24 for sealing, the rear cover 24 is detachably arranged in the opening 2111
On.
Above mentioned embodiment provide a kind of convenient solution detection devices, and to avoid secondary pollution, the detection device 2 is
Disposable form, the detection device 2 are used cooperatively with the internal test tubes 1 equipped with solution to be detected, for example,
Directly the test tubes 1 equipped with the PCR product solution after amplification can be mounted in detection device 2 and be detected, without opening
Test tubes 1 are sampled, and are avoided because volatilizing caused by opening lid or connecing the reasons such as place to testing result standard with air
The influence of true property, and conveniently solution can be detected.
It is also set up on the detection box 21 referring to Fig. 6 A to Fig. 8 to guarantee that test tubes 1 are mounted on set position
There is limiting device.Wherein, be connected with detection 21 edge of the box installation trough floor 2141 of one end of the mounting groove 214 is provided with
The connection of first positive stop strip 2144, side wall and top plate 213 is provided with the second positive stop strip to 214 internal stretch of mounting groove
2145, the gap of first positive stop strip 2144 and the second positive stop strip 2145 forms the socket for being inserted into test tubes, described
Test tubes 1 are detachably arranged on the detection box 21 by the socket.Certainly, the mounting groove 214 may also set up
Other are used to install the structure of the test tubes 1, do not do excessive limitation herein.
Preferably, 11 on both sides of the middle of tube body of the test tubes 1 is provided with the first retention bead 114, the mounting groove
The limit matched with first retention bead 114 is offered along the direction perpendicular to installation trough floor 2141 on 214 side wall
Position slot 2148.First retention bead 114 can be fastened in limiting slot 2148 during installation, further increase detection
The stability that test tube 1 is installed.
In addition, the end of the tube body 11 of the test tubes 1 is provided with positioning column 115, the end of the mounting groove 214 is set
It is equipped with the groove mutually matched with positioning column 115, limited post 2146, institute are respectively arranged on the side wall of the groove two sides
It states the installation of positioning column 115 in a groove and the side wall of 11 end of tube body is abutted against with the limited post 2146.
It is upper cover 22 and the scheme of installation for detecting box 21, the structure of the upper cover 22 is referring to Figure 10 A and figure referring to Fig. 9
10B, the upper cover 22 include cover board 220, are provided on the inner surface of the cover board 220 and cooperate with the contact pin 2142
Press pressure ring 222, it is described to be pressed in the detection process by pressure ring 222 in test tubes 1 as shown in the oval marks of Figure 11
It is provided with one end of sealing film 13, making sealing film 13, deformation occurs, and then is in contact with contact pin 2142 and by contact pin 2142
It is poked.
It is additionally provided with the first mounting post 223 on the inner surface of the cover board 220, is set on the top plate 213 of the detection box 21
It is equipped with the first mounting hole 2131, first mounting post 223, which can be embedded in, to be mounted in first mounting hole 2131.Meanwhile
On the top plate 213 of the detection box 21 and be connected with upper cover 22 both sides of the edge of one end of the bottom plate 211 open up respectively
There is the first seal groove 217.
Non-slip texture 227 is provided on the outer surface of the cover board 220, when the non-slip texture 227 can increase pressing
Friction, improve the operational stability of staff.
One end of the cover board 220 is pivoted on the detection box 21, and the other end can be overlapped on the top plate 213
On, it is provided with the side plate 221 that can be fastened on detection 21 two sides of box between both ends, the edge of the side plate 221 and takes
The end margin connect in the top plate 213 is separately provided for the first sealing strip 224 sealed, first sealing strip 224
It can fasten and be embedded in and be mounted in first seal groove 217, i.e., realize upper cover 22 and detection by way of labyrinth seal
Sealing between box 21, the schematic diagram of the labyrinth seal as shown in the circular mark of Figure 11 wherein, using labyrinth seal
Mode can make the same contacting external air of solution after entirely detection box prevents test tube to be punctured under normal pressure, it is molten that gas occurs
The pollution of the volatile materials such as glue.
In addition, at least one positive stop strip 226 is provided on the side plate 221 that the upper cover 22 is connected with bottom plate 211, it is described
Positive stop strip 226 can be abutted against with the lid 12 for the test tubes 1 being mounted in mounting groove 214.When fastening upper cover 22, institute
12 end of lid of test tubes 1 can be resisted by stating positive stop strip 226, to prevent the test tubes 1 when in use from mounting groove
It is exited in 214, realizes the effect of limit.
In addition, the outer surface of the side plate 221 is equipped with shallow slot 225, the shallow slot 225 provides a stress point, such as
When fruit occurs that the upper cover that will have been fastened 22 is needed to separate with detection box 21, staff can be exerted a force by the shallow slot 225 will
The upper cover 22 and detection box 21 of fastening are opened, to take out test tubes 1.
When test tubes 1 are mounted in mounting groove 214, upper cover 22 is fastened, it is described to press 222 pressing detection test tube 1 of pressure ring
Sealing film 13 where one end, and make its bending, the contact pin 2142 pokes the sealing film 13 in test tubes 1, into
And PCR product solution is flowed on chromatographic test paper 25 from outflow in test tubes 1 and by delivery hole 2143.First
Mounting post 223 and the first mounting hole 2131 cooperate, and can guarantee that upper cover 22 cannot come off, ensure that the steady of detection process
It is fixed.
The chromatographic test paper 25 is mounted on the inside of detection box 21, and the back of top plate 213 is arranged in the test paper locating part 216
Face.Specifically, being as shown in Figures 12 A and 12 B a kind of embodiment of test paper locating part 216, the back side both ends are respectively set
Have position-limiting drum 2160, be respectively set on the side wall of each position-limiting drum 2160 it is jagged and constitute it is described first support 2161,
The second retention bead 2163 for clamping the chromatographic test paper 25 is provided on the both sides of the edge of the notch, at least one
The initial makeup location third retention bead for limiting the chromatographic test paper 25 is provided on the side wall of position-limiting drum 2160
2164.Second support 2162 is the support beam being arranged between mounting groove 214 and observation window 215.
Wherein, the position-limiting drum 2160 can be set to cylindrical or cubic cylindricality etc., the limit of Figure 12 B illustrated embodiment
Position cylinder 2160 is cubic cylindricality, and is adaptively adjusted according to the structure of the mounting groove 214, can be guaranteed described
The both ends of chromatographic test paper 25 can be stable be mounted on described two position-limiting drums.Certainly, in addition to the position-limiting drum 2160, institute
Stating test paper locating part 216 can also be mounted on chromatographic test paper 25 inside detection box 21 using other structures.
One embodiment of the chromatographic test paper 25 used when detection is as shown in figure 13, the type of the chromatographic test paper 25 compared with
It is more, for the colloid gold test paper used when PCR product solution detects in the present embodiment.The colloid gold test paper is as chromatography
The one of which of test paper 25, including set gradually sample pad 251, bonding pad 252, chromatography layer 253 and water-absorption material backing strap 254,
The test paper locating part 216 includes that first support 2161 at the back side both ends of top plate 213 is arranged in and is arranged in the second of middle part
Support 2162, first support 2161 and the second support 2162 are located along the same line, the sample pad of the colloid gold test paper
One end where 251 is mounted in the first support 2161 corresponding with 214 one end of mounting groove, where water-absorption material backing strap 254
One end is mounted in another first support 2161, and the bonding pad 252 is in contact with the second support 2162.In addition, detecting
Other corresponding chromatographic test papers 25 can also be used when other are with volatile solution, do not do excessive restriction herein.
Figure 14 show chromatographic test paper 25 and detects the scheme of installation of box 21, specifically, when installation chromatographic test paper 25, institute
The initial position that 25 one or both ends of chromatographic test paper are installed by 2164 Duis of the third retention bead is stated to be defined, wherein
The sample pad 251 of the chromatographic test paper 25 is arranged below delivery hole 2143, and bonding pad 252 is in contact with the second support 2162,
And it chromatographs layer 253 to be oppositely arranged with observation window 215.Solution to be detected is flowed in sample pad 251 by the delivery hole 2143,
Chromatography layer 253 under the action of the water-absorption material backing strap 254 of 25 other end of chromatographic test paper among flow direction, and in the chromatography layer
Testing result is shown on 253.
Referring to Fig. 6 A and Fig. 6 B, the observation window 215 is the groove being arranged on top plate 213, including is arranged in groove-bottom
Window 2151 on plate, the words identification 2152 for characterizing testing result, the seal groove 2153 that bottom portion of groove edge is set
And the second mounting hole 2154 for installing test paper form 23.
Referring to Figure 15 A and Figure 15 B, the test paper form 23 is made of transparent plastics material, including observation piece 230, described
The both ends for observing the inner surface of piece 230 are provided with the second mounting post 231, and the inner surface edge setting for observing piece 230 is useful
In the second sealing strip 232 of Sealed Observation Window 215；Second mounting post 231 of the observation window 215 is mounted on second peace
It fills in hole 2154, and second sealing strip 232 can be embedded in and be mounted in the seal groove 2153, Figure 16 is the examination
The scheme of installation of paper form 23.The structure is still the principle using labyrinth seal, specific as shown in Figure 17.The examination
Paper form 23 not only can clearly observe testing result, but also the sealing and protection to observation window 215 may be implemented.
Simultaneously as the target of detection is different, and matched chromatographic test paper 25 is different, and the result of detection is by words identification
2152 indicate, referring to Figure 18, the display line and words identification 2152 of the chromatography layer 253, that is, C, T line or C, T1, T2 etc. one
One is corresponding, i.e., different testing results respectively corresponds different words identifications 2152, and staff can pass through observation window 215
Testing result is judged with test paper form 23.
Meanwhile the stability in order to guarantee the installation of chromatographic test paper 25, referring to Figure 19, the inner surface of the rear cover 24 is provided with
Multiple third mounting posts 241, it is described detection box 21 top plate 213 the back side on be provided with third mounting hole 219, the third
Mounting post 241, which can be embedded in, to be mounted in the third mounting hole 219.The both ends of 24 inner surface of rear cover are also respectively set
There is fixed lobe 243, the fixed lobe 243 can be abutted against with the chromatographic test paper 25 being mounted in the detection box 21.It is logical
It crosses and is matched with the first support 2161 and the second support 2162, stable being mounted on of chromatographic test paper 25 can be detected in box 21
Portion guarantees that process that is mobile and influencing detection will not be generated in detection process because of the chromatographic test paper 25.Wherein Figure 20 is institute
It states rear cover and detects the scheme of installation of box 21.
In addition, being also equipped with labyrinth seal knot between the bottom plate 211 and rear cover 24 referring to Figure 12 A, 12B and Figure 15 A
Structure, specifically, the sealing structure includes the third sealing strip 242 and the bottom plate 211 that 24 edge of rear cover is arranged in
The edge of opening 2111 is provided with third seal groove 2112, and the third sealing strip 242 can be embedded in that be mounted on the third close
In sealing groove 2112.The labyrinth seal structure is as shown in figure 21, can effectively realize the sealing of detection 21 bottom of box, prevent
Extra PCR product solution is overflowed from the bottom of detection box 21.
Sealing structure can effectively realize the sealing of detection device, prevent as shown in figure 22 at comprehensive described above three
Solution overflows when poking sealing film 13 in detection process, has blocked solution to the harm of operator and the pollution of environment, together
When also can be avoided because volatile matter content change caused by testing result inaccuracy or it is unstable.
The application further comprises a kind of solution detection method, and the detection method is realized by using above-mentioned detection device
, specifically, the detection method includes the following steps.
By solution to be detected in test tubes 1；
One end that the test tubes 1 are provided with sealing film 13 is mounted in mounting groove 214；
Upper cover 22 and detection box 21 are fastened, so that contact pin 2142 is poked sealing film 13, solution to be detected passes through delivery hole
2143 flow into the sample pad 251 of chromatographic test paper 25；
Testing result is judged by observation window 215.Remaining with the existing consistent detecting step of conventional detection mode herein
Excessive limitation and explanation are not done.
It is illustrated using PCR product solution as specific embodiment, it is molten to reagent by reagent solution in test tubes 1
Specific nucleic acid fragment in liquid is proliferated, and the solution after proliferation is PCR product solution to be detected.When detection, it can will be equipped with
The test tubes 1 of PCR solution, which are directly installed in detection device, to be detected, and is eliminated and is opened what test tubes 1 were sampled
Step, meanwhile, also it is avoided that PCR product solution occurs the feelings of testing result inaccuracy with the air place of connecing after the opening of lid 12
Condition simplifies operating procedure, also improves the accuracy of detection.
The embodiment provides a kind of solution detection device and corresponding detection method, the detection device can be straight
It connects and is used cooperatively with equipped with the test tubes of solution to be detected, be provided with and can be used in installing test tubes and chromatographic test paper
Box and upper cover and rear cover for sealing are detected, the internal test tubes equipped with solution to be detected directly can be inserted into detection dress
In setting, upper cover pressing is buckled, the detection box is internally provided with the mounting groove of contact pin and delivery hole, realizes the environment in sealing
Under the solution in test tubes is detected, eliminate and extract solution from test tube using tools such as dropper, liquid-transfering guns and move
The process of chromatographic test paper is moved, mode of operation is efficient and convenient.Meanwhile it being filled by the detection that detection box, upper cover and rear cover assemble
It sets, realizes closed environment, in addition to general solution, be also particularly suitable for the detection with volatile materials solution, it can
It is effectively prevent the spilling of volatile materials, avoids Aerosol Pollution, makes subsequent experiment since Aerosol Pollution leads to testing result
The probability of inaccuracy substantially reduces, while avoiding the harm of operator and the pollution of environment.
Those skilled in the art after considering the specification and implementing the invention disclosed here, will readily occur to of the invention its
Its embodiment.The present application is intended to cover any variations, uses, or adaptations of the invention, these modifications are used
Way or adaptive change follow general principle of the invention and including the present invention it is undocumented in the art known in
Common sense or conventional techniques.The description and examples are only to be considered as illustrative, and the scope of the present invention is only by appended right
It is required that limit.
Claims (14)
1. a kind of solution detection device, it is characterised in that: the detection device (2) is used cooperatively with test tubes (1), the inspection
Testing tube (1) includes tube body (11) and lid (12), and the lower end outer wall of the tube body (11) is the sealing film of cladding setting
(13), the detection device (2) includes for installing the detection box (21) of the test tubes (1), upper cover 22, the upper cover
(22) it is pivoted on one end bottom of detection box (21) and can be fastened on the detection box (21), wherein
It is set as stepped in the detection box (21), including bottom plate (211), the side wall (212) in bottom plate (211) one end is set
And the top plate (213) being arranged at the top of side wall (212),
The peace for installing the test tubes (1) is offered on the top plate (213) and upper cover (22) adjacent one end top surface
The edge of tankage (214), the mounting groove (214) is connected with detection box (21) edge；It is set in installation trough floor (2141)
It is equipped with the contact pin (2142) for poking the sealing film (13), the installation trough floor in the contact pin (2142) side
(2141) delivery hole (2143) for being connected to the mounting groove (214) Yu chromatographic test paper (25) is offered on；
The observation window of the state change for observing chromatographic test paper (25) is also provided on the top surface other end of the top plate (213)
(215), the test paper form (23) for sealing is installed on the observation window 215；
At the back side of the top plate (213), i.e., described detection box (21) is internally provided with for installing chromatographic test paper (25)
Test paper locating part (216)；
One end of the bottom plate (211) has been pivotally mounted upper cover (22), and the other end is offered for placing the chromatographic test paper
(25) opening (2111), and configured with the rear cover (24) for sealing, the rear cover (24) is detachably arranged in described open
On mouth (2111).
2. solution detection device according to claim 1, it is characterised in that:
Be connected with detection box (21) edge installation trough floor (2141) of one end of the mounting groove (214) is provided with the first limit
Item (2144), the connections of the side wall of mounting groove (214) and top plate (213) are provided with the to mounting groove (214) internal stretch
The gap of two positive stop strips (2145), first positive stop strip (2144) and the second positive stop strip (2145) is formed for being inserted into detection examination
The socket of pipe.
3. solution detection device according to claim 2, it is characterised in that:
Tube body (11) on both sides of the middle of the test tubes (1) is provided with the first retention bead (114),
It is offered and first limit on the side wall of the mounting groove (214) along the direction perpendicular to installation trough floor (2141)
The limiting slot (2148) that raised (114) match.
4. solution detection device according to claim 1 or 2, it is characterised in that:
The end of the tube body (11) of the test tubes (1) is provided with positioning column (115)；
The end of the mounting groove (214) is provided with and positioning column (115) mutually matched groove, the side of the groove two sides
It is respectively arranged on wall limited post (2146), the positioning column (115) installation is in a groove and the side wall of tube body (11) end
It is abutted against with the limited post (2146).
5. solution detection device according to claim 1 or 2, it is characterised in that:
One end that the contact pin (2142) is connected with installation trough floor (2141) is cylindrical structure, and the other end is pyramidal structure,
Radial direction on the outer surface of the contact pin (2142) along cylinder is offered for guiding solution to be detected to flow to delivery hole (2143)
Intake chute (2147).
6. solution detection device according to claim 1, it is characterised in that:
The chromatographic test paper (25) can be colloid gold test paper, the colloid gold test paper include the sample pad (251) set gradually,
Bonding pad (252), chromatography layer (253) and water-absorption material backing strap (254),
The test paper locating part (216) includes that first support (2161) and setting of the setting at the back side both ends of top plate (213) exist
Second support (2162) at middle part,
First support (2161) and the first support (2162) are located along the same line, the sample pad of the colloid gold test paper
(251) one end where is mounted in the first support (2161) corresponding with mounting groove (214) one end, water-absorption material backing strap
(254) one end where is mounted in another first support (2161), the bonding pad (252) and first support (2162) phase
Contact.
7. solution detection device according to claim 6, it is characterised in that:
The back side both ends of the top plate (213) are respectively arranged with position-limiting drum (2160), the side wall of each position-limiting drum (2160)
On be respectively set jagged and constitute first support (2161), be provided with for clamping on the both sides of the edge of the notch
State the second retention bead (2163) of chromatographic test paper (25)；
The initial makeup location for limiting the chromatographic test paper (25) is provided on the side wall of at least one position-limiting drum (2160)
Third retention bead (2164).
8. solution detection device according to claim 1, it is characterised in that:
The upper cover (22) includes cover board (220), is provided on the inner surface of the cover board (220) and the contact pin (2142) phase
Mutually cooperate by pressure ring (222), be provided with non-slip texture (227) on outer surface；
It is additionally provided with the first mounting post (223) on the inner surface of the cover board (220), the top plate (213) of detection box (21)
On be additionally provided with the first mounting hole (2131), first mounting post (223), which can be embedded in, is mounted on first mounting hole
(2131) in.
9. solution detection device according to claim 8, it is characterised in that:
On the top plate (213) of detection box (21) and the bottom plate (211) is connected the plate face two of one end with upper cover (22)
Side edge offers the first seal groove (217) respectively；
One end of the cover board (220) is pivoted on the detection box (21), and the other end can be overlapped on the top plate (213)
On, be provided with the side plate (221) that can be fastened on described detection box (21) two sides between both ends, the edge of the side plate 221 with
And be overlapped on the top plate (213) an end margin be separately provided for sealing the first sealing strip (224), described first is close
Strip of paper used for sealing (224) can be fastened and be embedded in and be mounted in first seal groove (217).
10. solution detection device according to claim 9, it is characterised in that:
It is provided at least one positive stop strip (226) on the side plate (221) that the upper cover (22) is connected with bottom plate (211), it is described
Positive stop strip (226) can be abutted against with the lid (12) for the test tubes (1) being mounted in mounting groove (214).
11. solution detection device according to claim 1, it is characterised in that:
The observation window (215) is the groove being arranged on top plate (213), including the window being arranged in groove base plate
(2151), for characterize the words identification (2152) of testing result, be arranged in bottom portion of groove edge seal groove (2153) and
For installing the second mounting hole (2154) of test paper form (23)；
The test paper form (23) is made of transparent plastics material, including observation piece (230), the interior table of observation piece (230)
The both ends in face are provided with the second mounting post (231), and the inner surface edge for observing piece (230) is provided with for Sealed Observation Window
(215) the second sealing strip (232)；
The second mounting post (231) of the observation window (215) is mounted in second mounting hole (2154), and described second
Sealing strip (232), which can be embedded in, to be mounted in the seal groove (2153).
12. solution detection device according to claim 1, it is characterised in that:
The inner surface of the rear cover (24) is provided with multiple third mounting posts (241), the top plate (213) of detection box (21)
It is provided on the back side third mounting hole (219), the third mounting post (241), which can be embedded in, is mounted on the third mounting hole
(219) in；
The both ends of rear cover (24) inner surface are also respectively provided with fixed lobe (243), and the fixed lobe (243) can be with
The chromatographic test paper (25) being mounted in detection box (21) abuts against；
The edge of the rear cover (24) is additionally provided with the third sealing strip (242) for sealing, the opening of the bottom plate (211)
(2111) edge is provided with third seal groove (2112), and the third sealing strip (242), which can be embedded in, is mounted on the third
In seal groove (2112).
13. solution detection device according to claim 1, it is characterised in that:
One end of the bottom plate (211) is provided with hemispherical protuberances or mounting hole, one end setting of the upper cover (22) and institute
State hemispherical protuberances or pivotal axis (228) that mounting hole matches, the pivotal axis (228) is pivoted on the bottom
One end of plate (211).
14. a kind of solution detection method, which is characterized in that the method includes,
By solution to be detected in test tubes (1),
One end that the test tubes (1) are provided with sealing film (13) is mounted in mounting groove (214)；
Upper cover (22) and detection box (21) are fastened, press the detection by pressure ring (222) on upper cover (22) inner surface
Test tube 1 pokes contact pin (2142) sealing film (13), and solution to be detected flows into chromatographic test paper (25) by delivery hole (2143)
Sample pad (251) on.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710770834.4A CN109425573B (en) | 2017-08-31 | 2017-08-31 | Solution detection device and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710770834.4A CN109425573B (en) | 2017-08-31 | 2017-08-31 | Solution detection device and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109425573A true CN109425573A (en) | 2019-03-05 |
| CN109425573B CN109425573B (en) | 2021-08-31 |
Family
ID=65505181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710770834.4A Active CN109425573B (en) | 2017-08-31 | 2017-08-31 | Solution detection device and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109425573B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111272744A (en) * | 2020-01-21 | 2020-06-12 | 北京理工大学珠海学院 | Detection apparatus for antibiotic in detection cow's milk |
| CN111965175A (en) * | 2020-08-12 | 2020-11-20 | 武汉普利赛斯科技有限公司 | Preparation method of AIDS blood test paper |
| CN113484514A (en) * | 2021-03-25 | 2021-10-08 | 江苏硕世生物科技股份有限公司 | Test paper detection auxiliary device |
| CN117129671A (en) * | 2023-10-26 | 2023-11-28 | 苏州星安生物科技有限公司 | Excrement sample collection and detection integrated device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5376337A (en) * | 1990-12-18 | 1994-12-27 | Seymour; Eugene H. | Saliva sampling device and sample adequacy system |
| CN1888902A (en) * | 2006-08-11 | 2007-01-03 | 杭州优思达生物技术有限公司 | Full closed target nucleic amplifier fast testing device |
| CN103267839A (en) * | 2013-04-28 | 2013-08-28 | 上海快灵生物科技有限公司 | Enclosed chromatographic test strip plastic card box |
| CN105836240A (en) * | 2016-03-14 | 2016-08-10 | 上海快灵生物科技有限公司 | Tube breaking mechanism, sealed test tube assembly comprising tube breaking mechanism and application thereof |
-
2017
- 2017-08-31 CN CN201710770834.4A patent/CN109425573B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5376337A (en) * | 1990-12-18 | 1994-12-27 | Seymour; Eugene H. | Saliva sampling device and sample adequacy system |
| CN1888902A (en) * | 2006-08-11 | 2007-01-03 | 杭州优思达生物技术有限公司 | Full closed target nucleic amplifier fast testing device |
| CN103267839A (en) * | 2013-04-28 | 2013-08-28 | 上海快灵生物科技有限公司 | Enclosed chromatographic test strip plastic card box |
| CN105836240A (en) * | 2016-03-14 | 2016-08-10 | 上海快灵生物科技有限公司 | Tube breaking mechanism, sealed test tube assembly comprising tube breaking mechanism and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111272744A (en) * | 2020-01-21 | 2020-06-12 | 北京理工大学珠海学院 | Detection apparatus for antibiotic in detection cow's milk |
| CN111272744B (en) * | 2020-01-21 | 2022-03-11 | 北京理工大学珠海学院 | Detection apparatus for antibiotic in detection cow's milk |
| CN111965175A (en) * | 2020-08-12 | 2020-11-20 | 武汉普利赛斯科技有限公司 | Preparation method of AIDS blood test paper |
| CN113484514A (en) * | 2021-03-25 | 2021-10-08 | 江苏硕世生物科技股份有限公司 | Test paper detection auxiliary device |
| CN117129671A (en) * | 2023-10-26 | 2023-11-28 | 苏州星安生物科技有限公司 | Excrement sample collection and detection integrated device |
| CN117129671B (en) * | 2023-10-26 | 2024-01-23 | 苏州星安生物科技有限公司 | Excrement sample collection and detection integrated device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109425573B (en) | 2021-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109425573A (en) | A kind of solution detection device and detection method | |
| US11883824B2 (en) | Assay using different spacing heights | |
| EP3380837B1 (en) | Assay device | |
| US6805837B2 (en) | Assaying device and method for in field urinalysis | |
| CN101263392B (en) | Microchannel chip | |
| US7645424B2 (en) | Reagent cuvette | |
| US20170347935A1 (en) | Rotatable disk-shaped fluid sample collection device | |
| US20180272331A1 (en) | Device for preparing and dispensing biological sample | |
| CA2597177A1 (en) | Immunoassay test device and method of use | |
| US11154856B2 (en) | Test device | |
| EP4012408B1 (en) | Sample collection and detection device and method | |
| US6805838B2 (en) | Assaying device and method for in field urinalysis | |
| Deprez et al. | Automation in Microsampling: At Your Fingertips? | |
| US20070275456A1 (en) | Diagnostic Testing Apparatus | |
| JP7217133B2 (en) | inspection device | |
| CN208013236U (en) | The kit quantitatively detected for colloidal gold | |
| JP6932617B2 (en) | Inspection device | |
| US20240157359A1 (en) | Systems and methods for sample analysis | |
| KR100988458B1 (en) | Semi-quantitative immunological detection device with differential antibodies or substrates | |
| WO2020140185A1 (en) | Assay device | |
| CN109752532B (en) | Incubation sample loading device based on multi-channel immunochromatography detection | |
| KR20210049662A (en) | Apparatus capable of quantitative sampling | |
| US20190120829A1 (en) | Mixing method and mixing apparatus for particle agglutination | |
| IE84434B1 (en) | A reagent cuvette |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 100176 floor 01-5, building 16, yard 25, Jinghai 4th Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing Patentee after: Beijing jinnuomi Technology Co.,Ltd. Address before: 100176 room 407, building 2, No. 19, Yongchang South Road, Yizhuang Economic Development Zone, Daxing District, Beijing Patentee before: BEIJING GENOME BIOTECHNOLOGY CO.,LTD. |