CN109394376B - Tooth staining risk assessment method - Google Patents
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- CN109394376B CN109394376B CN201811162858.2A CN201811162858A CN109394376B CN 109394376 B CN109394376 B CN 109394376B CN 201811162858 A CN201811162858 A CN 201811162858A CN 109394376 B CN109394376 B CN 109394376B
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Abstract
The invention relates to a method for evaluating tooth staining risk assessment, in particular to a staining risk assessment method for human teeth by foods, medicines, oral care products and the like, which comprises the following steps: step 1: establishing a laboratory use model of human used food, medicine, oral care products and other entrance products; step 2: performing baseline test on the experimental tooth sample, and measuring the L values of all untreated experimental tooth samples by using a color difference meter; and step 3: placing the experimental tooth sample in a model for multiple times, placing the experimental tooth sample in artificial saliva in a space between each time of treatment, and placing the artificial saliva in a constant temperature and humidity box at 37 ℃; and 4, step 4: and (3) measuring the L values of all the experimental tooth samples processed in the step (3) by using a color difference meter, performing data analysis on the L values of the experimental tooth samples before processing and the L values after processing, and performing risk assessment on the tooth staining condition. By using the scheme of the invention, the staining risk of products such as food, medicines, oral care products and the like which enter the mouth to teeth can be effectively evaluated.
Description
Technical Field
The invention relates to a method for evaluating tooth staining risk assessment, in particular to a staining risk assessment method for human teeth of foods, medicines, oral care products and the like.
Background
There are many causes of tooth staining, generally classified as endogenous and exogenous. The exogenous coloring is mainly from smoking, drinking tea, drinking coffee and eating colored food, colored substances are adsorbed on organic films on the surfaces of teeth, the dyed films cannot be effectively removed in time, and the colored films are gradually deposited on the surfaces of tooth enamel along with time to form stains which are difficult to remove, and then the stains are gradually developed into dental calculus or dental plaque which is difficult to remove, so that the teeth turn yellow; endogenous staining refers to changes in tooth color caused by internal or pharmaceutical factors.
The white state of the teeth is the most basic condition for the aesthetic image of the human body, and the two kinds of coloring not only damage the aesthetic image of the human body, but also seriously affect the health state of the oral cavity of the human body.
At present, although there are related literature reports on the research of dental staining by products such as food, medicine, oral care products and the like, the research is from the aspects of staining principle and restoration, a systematic and complete dental staining risk assessment method is not provided, dental staining seriously affects human aesthetics for consumers, and for product research and development companies, the consumer complaint risk after the products are on the market is increased, so that a systematically and conveniently dental staining risk assessment device and a testing method for assessing the dental staining risk of each product are urgently needed.
Disclosure of Invention
The invention aims to provide a tooth staining risk assessment method for assessing the staining risk of products such as food, medicines, oral care products and the like which enter the mouth to teeth.
In order to achieve the purpose, the basic scheme of the invention is as follows: a method of assessing risk of tooth staining comprising the steps of:
step 1: establishing human diet models of food, medicine and beverage eaten by human beings and human tooth brushing models of human beings using oral care products, wherein the product to be tested is the food, the medicine and the beverage, and the human diet model is selected if the product to be tested is the oral care product;
step 2: performing baseline test on the experimental tooth samples, measuring the L values of all unprocessed experimental tooth samples by using a color difference meter, and grouping;
and step 3: selecting the model set in the step 1 according to a product to be tested, placing the experimental tooth sample in the model to be processed for multiple times, placing the experimental tooth sample in artificial saliva in a space between each time of processing, and placing the artificial saliva in a constant temperature and humidity box at 37 ℃;
and 4, step 4: and (3) measuring the L values of all the experimental tooth samples processed in the step (3) by using a color difference meter, performing data analysis on the L values of the experimental tooth samples before processing and the L values after processing, and performing risk assessment on the tooth staining condition.
The beneficial effect of this basic scheme lies in:
1. according to the tooth staining risk assessment method, the estimation of the product on the tooth staining risk is obtained by establishing a simple and effective assessment method, the risk is known without waiting for the occurrence of problems in the product on the market, and therefore the product risk is effectively avoided.
2. The tooth staining risk assessment method is beneficial to systematic research on product safety, avoids the staining side effect generated by products, and is rapid, simple, convenient, time-saving and labor-saving.
Further, in step 4, statistical analysis was performed using statistical software SPSS, and when P <0.05, the teeth were at risk of staining. And statistical software SPSS is used for data analysis, so that the data analysis efficiency is effectively improved.
Further, the human diet model in the step 1 comprises a bracket, a fixing unit and a driving part for driving the fixing unit to rotate, wherein the fixing unit is rotationally connected to the bracket and is used for fixing the experimental tooth sample; the bracket is provided with a coloring box with an opening at the upper part, the coloring box is positioned below the fixing unit, and the fixing unit can enable the experimental tooth sample to be positioned in the coloring box in the rotating process; the bracket is provided with a baking lamp positioned above the fixing unit.
When the human diet model is used, coloring liquid (such as soluble food, beverage or medicine) is poured into the coloring box, the experimental tooth sample is fixed through the fixing unit, the driving part is opened to enable the fixing unit to rotate, the experimental tooth sample can be located in the coloring box in the rotating process of the fixing unit, and the human diet model can better simulate the process of human food.
Further, the fixing unit comprises a mounting shaft which is rotatably connected to the bracket, and the mounting shaft is driven by the driving part; the both ends of installation axle are equipped with the disc, and open all around of disc has the recess, and the recess on two discs corresponds mutually, is equipped with same foraminiferous fixed strip on the recess that two discs correspond, and the fixed strip is used for fixed experiment tooth sample.
Further, the driving part comprises a motor and a transmission, an output shaft of the motor is connected with an input shaft of the transmission, and an output shaft of the transmission is fixedly connected with the mounting shaft. The rotating speed of the mounting shaft is adjusted by the transmission, so that the rotating speed meets the actual requirement.
Further, the human body tooth brushing model in the step 1 comprises a bottom plate, a pressure sensor and a fixing plate installed on the bottom plate, a threaded rod is fixed on the bottom plate, a fixing nut is connected onto the threaded rod in a threaded mode, an installation part is connected onto the fixing nut in a rotating mode, and a laser range finder and an electric toothbrush clamping mechanism used for clamping an electric toothbrush are arranged on the installation part.
The fixed plate is used for fixed experiment tooth sample, utilizes electric toothbrush fixture fixed with electric toothbrush, before using this model, utilizes laser range finder and pressure sensor test electric toothbrush fixture earlier when the different distances of fixed plate electric toothbrush produces the pressure of experiment tooth sample, corrects pressure and distance before using. Smearing the oral care product on an electric toothbrush, starting the electric toothbrush, and cleaning the experimental tooth sample by the electric toothbrush. The model can effectively simulate the process of human tooth brushing.
Furthermore, a through rectangular hole is formed in the fixing plate and used for fixing the experimental tooth sample; the bottom plate is provided with a limit groove matched with the fixing plate, the fixing plate is clamped in the limit groove, and the height of the fixing plate is lower than that of the limit groove. The fixed plate is used for fixed experiment tooth sample, and the fixed plate block is at the spacing inslot, and the position of spacing groove can effectual locking fixed plate avoids electric toothbrush during operation experiment tooth sample to take place to slide.
Further, the mounting part comprises a first holding clamp rotationally connected with the fixing nut, and the laser range finder is arranged on the first holding clamp; a horizontal connecting rod is arranged on the first holding clamp, and an electric toothbrush clamping mechanism is arranged on the connecting rod; the electric toothbrush clamping mechanism comprises a second holding clamp and a third holding clamp arranged on the second holding clamp, a first clamping opening is formed in the lower end of the second holding clamp, and a second clamping opening opposite to the first clamping opening is formed in the lower end of the third holding clamp. When the electric toothbrush is fixed, the brush handle of the electric toothbrush passes through the first clamping port, the second clamping port of the brush head of the electric toothbrush is used for fixing the brush handle of the electric toothbrush, and the second clamping port is used for fixing the brush head of the electric toothbrush, so that the electric toothbrush can be clamped and fixed effectively.
Further, in step 3, when the test tooth sample is treated with the human tooth-brushing model, the pressure of the electric toothbrush on the test tooth sample is 140 to 160 g. The pressure is set to the parameters, so that human tooth brushing can be well simulated, and the obtained data is more accurate.
Drawings
FIG. 1 is a schematic view showing the construction of a model of a human tooth brush according to example 1;
FIG. 2 is an exploded view of FIG. 1;
fig. 3 is a schematic structural diagram of the human diet model in example 2.
Detailed Description
The following is further detailed by way of specific embodiments:
the reference numerals in figures 1 to 3 of the specification include: laser range finder 1, pressure sensor 2, fixed plate 3, bottom plate 4, electric toothbrush 5, third embrace and press from both sides 6, second embrace and press from both sides 7, first embrace and press from both sides 8, fixation nut 9, threaded rod 10, support 11, installation axle 12, disc 13, roast lamp 14, fixed strip 15, painted box 16.
Example 1
In this example, a toothpaste containing a colored Chinese medicinal component was used as a test product to evaluate the risk of staining teeth with the toothpaste containing a colored Chinese medicinal component.
A method of assessing risk of tooth staining comprising the steps of:
step 1: a human body tooth brushing model is selected for testing. As shown in figures 1 and 2, the human body tooth brushing model comprises a bottom plate 4, a pressure sensor 2 and a fixing plate 3 which is detachably arranged on the bottom plate 4, wherein a rectangular hole is formed in the fixing plate 3 and penetrates through the fixing plate, and the rectangular hole is used for fixing a test tooth sample. The threaded rod 10 perpendicular to the bottom plate 4 is welded on the bottom plate 4, the threaded rod 10 is in threaded connection with the fixing nut 9, and the fixing nut 9 can ascend and descend along the threaded rod 10 when the fixing nut 9 is rotated. The fixing nut 9 is rotatably connected with an installation part, and the installation part is provided with a laser range finder 1 and an electric toothbrush clamping mechanism for clamping the electric toothbrush 5. The installation department includes and presss from both sides 8 with the first armful of 9 rotation connections of fixation nut, is equipped with on the first armful clamp 8 to be used for pressing from both sides the fixed fixing bolt who tightly fixes. The laser range finder 1 is fixed on the first holding clamp 8 by means of screws and the like; be fixed with the connecting rod of level setting on the first armful of clamp 8, the cross-section of connecting rod is trapezoidal, triangle-shaped or square, and the preferred is trapezoidal in this embodiment. The electric toothbrush clamping mechanism is connected to the connecting rod in a sliding mode, and when the electric toothbrush clamping mechanism slides to a proper position, the electric toothbrush 5 clamping mechanism can be fixed to the connecting rod through fasteners such as bolts. The electric toothbrush clamping mechanism comprises a second holding clamp 7 and a third holding clamp 6, an extension rod horizontally arranged is fixed on the second holding clamp 7, and the third holding clamp 6 is connected to the second holding clamp 7 through the extension rod. The lower extreme that the second was embraced and is pressed from both sides 7 is equipped with first centre gripping mouth, and the lower extreme that the third was embraced and is pressed from both sides 6 is equipped with the second centre gripping mouth relative with first centre gripping mouth, and first centre gripping mouth is used for fixing electric toothbrush's 5 brush holder, and the second centre gripping mouth is used for fixing electric toothbrush's 5 brush head, all is equipped with the rubber circle between first centre gripping mouth and second centre gripping mouth and the electric toothbrush 5. The bottom plate 4 is provided with a rectangular limiting groove matched with the fixing plate 3, the fixing plate 3 is clamped in the limiting groove, and the height of the fixing plate 3 is lower than that of the limiting groove.
Step 2: adjusting the clamping position of the electric toothbrush and calibrating the pressure of the brush head of the electric toothbrush. Utilize electric toothbrush fixture 5 fixed, fix experiment tooth sample in the rectangle hole, place pressure sensors on bottom plate 4, rotate fixation nut 9, adjust 5 level of electric toothbrush downwards, 5 brush heads of electric toothbrush contact pressure sensors and begin to produce pressure data, can take out pressure sensors with the distance one-by-one record of the laser range finder 1 that pressure data corresponds this moment, after the pressure correction, adjust corresponding pressure condition through the distance of laser range finder 1. The pressure of the electric toothbrush brush head on the experimental tooth sample is controlled to be 150g, and the pressure is the pressure generated by the brush head on the experimental tooth sample and is the total pressure.
And step 3: and (4) performing baseline test on the experimental tooth samples, measuring the L values of all untreated experimental tooth samples by using a color difference meter, recording and grouping.
Step 4: fixing the experimental tooth sample on a fixing plate, preparing toothpaste containing color traditional Chinese medicine components into toothpaste slurry, pouring the toothpaste slurry into a limiting groove, starting an electric toothbrush, and cleaning the experimental tooth sample by the electric toothbrush. Each brushing test is carried out for 30 minutes and repeated for 30 times, and the time interval between each brushing test is 4 h. During the time between brushing test treatments, the experimental tooth sample is placed in artificial saliva and placed in a constant temperature and humidity chamber at 37 ℃;
and 5: and (4) measuring the L values of all the experimental tooth samples processed in the step (4) by using a color difference meter, and performing statistical analysis on the L values of the experimental tooth samples before and after processing by using statistical software SPSS to perform risk assessment on the tooth staining condition.
The toothpaste containing the colored traditional Chinese medicine components is adopted for testing according to the method, and the measurement results of the obtained colorimeter are as follows:
table 1: color data before and after enamel sample test
The analysis results using the SPSS software are as follows:
the L value of the enamel sample is different before and after tooth brushing by a paired t test of t being 7.031, P being 0.000 and being less than the significance level of 0.05, and the L value of the enamel sample is reduced by 1.59 after tooth brushing by the toothpaste containing the color Chinese medicinal components, namely the toothpaste containing the color Chinese medicinal components has the risk of staining the enamel sample.
Example 2
In this example, black tea was used as a test product to assess the risk of staining teeth with black tea.
A method of assessing risk of tooth staining comprising the steps of:
step 1: human diet models were selected for the experiments. As shown in fig. 3, the human diet model includes a bracket 11, a fixing unit rotatably coupled to the bracket 11, and a driving part for driving the fixing unit to rotate, the fixing unit being used to fix the experimental dental specimen. The side of the bracket 11 is provided with a sliding chute which is horizontally arranged, the sliding chute is connected with a coloring box 16 which is positioned below the fixing unit in a sliding way, and the upper part of the coloring box 16 is opened. The color box 16 can enable the experimental tooth sample to be positioned in the color box 16 in the process of rotating the fixing unit of the color box 16; the bracket 11 is provided with a baking lamp 14 positioned above the fixing unit, and the baking lamp 14 can accelerate the drying of the experimental tooth sample. The fixing unit comprises a mounting shaft 12 which is rotatably connected to the support 11, discs 13 are arranged at two ends of the mounting shaft 12, a plurality of uniformly distributed grooves are formed in the periphery of each disc 13, and the grooves in the two discs 13 correspond to each other. The same fixing strip 15 with holes is arranged on the corresponding grooves of the two disks 13, the fixing strip 15 is fixed in the groove of the disk 13 through screws, and the holes in the fixing strip 15 are used for fixing experimental tooth samples. The driving part comprises a motor and a speed changer, wherein an output shaft of the motor is connected with an input shaft of the speed changer, and an output shaft of the speed changer is fixedly connected with the mounting shaft 12.
Step 2: and (4) performing baseline test on the experimental tooth samples, measuring the L values of all untreated experimental tooth samples by using a color difference meter, recording and grouping.
And step 3: fixing the experimental tooth sample on the hole of the fixing strip 15, starting the motor, driving the fixing unit to rotate by the motor, and enabling the experimental tooth sample to be positioned in the coloring box 16 in the rotating process of the fixing unit; the treatment was repeated 30 times for 30 minutes, with 4h intervals between each brushing. At the time between each treatment, the experimental tooth samples were placed in artificial saliva and placed in a 37 ℃ constant temperature and humidity chamber.
And 4, step 4: and (3) measuring the L values of all the experimental tooth samples processed in the step (3) by using a color difference meter, and performing statistical analysis on the L values of the experimental tooth samples before and after processing by using statistical software SPSS to perform risk assessment on the tooth staining condition.
According to the method, different varieties of black tea liquid and distilled water are adopted to test the tooth sample, and the measurement results of the obtained color difference meter are as follows:
TABLE 1: colorimetric data before and after experimental dental sample test
After the test of the tooth sample, the L value before the tooth sample is processed is a base line, and the analysis and comparison among the delta L value groups are as follows:
and (4) experimental conclusion:
after the tooth sample test, the delta L values of the black tea A treatment group, the black tea B treatment group and the distilled water treatment group have significant statistical difference; there was no significant statistical difference in Δ L values between the black tea B treated group and the distilled water treated group. It can be concluded that black tea a is at risk of staining enamel and black tea B is not at risk of staining enamel.
Claims (7)
1. A method of assessing risk of tooth staining, comprising the steps of:
step 1: establishing human diet models of food, medicine and beverage for human beings and human tooth brushing models of human beings using oral care products, wherein the product to be tested is food, medicine and beverage, and the human diet model is selected for the product to be tested is the oral care product; the human diet model comprises a bracket, a fixing unit and a driving part for driving the fixing unit to rotate, wherein the fixing unit is rotationally connected to the bracket and is used for fixing the experimental tooth sample; the bracket is provided with a coloring box with an opening at the upper part, the coloring box is positioned below the fixing unit, and the fixing unit can enable the experimental tooth sample to be positioned in the coloring box in the rotating process; the bracket is provided with a baking lamp positioned above the fixing unit; the human body tooth brushing model comprises a bottom plate, a pressure sensor and a fixing plate arranged on the bottom plate, wherein a threaded rod is fixed on the bottom plate, a fixing nut is connected onto the threaded rod in a threaded manner, an installation part is rotatably connected onto the fixing nut, and a laser range finder and an electric toothbrush clamping mechanism for clamping an electric toothbrush are arranged on the installation part;
step 2: performing baseline test on the experimental tooth samples, measuring the L values of all unprocessed experimental tooth samples by using a color difference meter, and grouping;
and step 3: selecting the model set in the step 1 according to a product to be tested, placing the experimental tooth sample in the model to be processed for multiple times, placing the experimental tooth sample in artificial saliva in a space between each time of processing, and placing the artificial saliva in a constant temperature and humidity box at 37 ℃;
and 4, step 4: and (3) measuring the L values of all the experimental tooth samples processed in the step (3) by using a color difference meter, performing data analysis on the L values of the experimental tooth samples before processing and the L values after processing, and performing risk assessment on the tooth staining condition.
2. A method for assessing the risk of tooth staining according to claim 1, wherein: in step 4, statistical analysis was performed using statistical software SPSS, and when P <0.05, the teeth were at risk of staining.
3. A method for assessing the risk of tooth staining according to claim 1, wherein: the fixing unit comprises a mounting shaft which is rotationally connected to the bracket, and the mounting shaft is driven by the driving part; the both ends of installation axle are equipped with the disc, and open all around of disc has the recess, and the recess on two discs corresponds mutually, is equipped with same foraminiferous fixed strip on the recess that two discs correspond, and the fixed strip is used for fixed experiment tooth sample.
4. A method for assessing the risk of tooth staining according to claim 3, wherein: the driving part comprises a motor and a speed changer, an output shaft of the motor is connected with an input shaft of the speed changer, and an output shaft of the speed changer is fixedly connected with the mounting shaft.
5. A method for assessing the risk of tooth staining according to claim 1, wherein: a rectangular hole is formed in the fixing plate and used for fixing an experimental tooth sample; the bottom plate is provided with a limit groove matched with the fixing plate, the fixing plate is clamped in the limit groove, and the height of the fixing plate is lower than that of the limit groove.
6. A method for assessing the risk of tooth staining according to claim 5, wherein: the mounting part comprises a first holding clamp rotationally connected with the fixing nut, and the laser range finder is arranged on the first holding clamp; a horizontal connecting rod is arranged on the first holding clamp, and an electric toothbrush clamping mechanism is arranged on the connecting rod; the electric toothbrush clamping mechanism comprises a second holding clamp and a third holding clamp arranged on the second holding clamp, a first clamping opening is formed in the lower end of the second holding clamp, and a second clamping opening opposite to the first clamping opening is formed in the lower end of the third holding clamp.
7. A method for assessing the risk of tooth staining according to claim 6, wherein: in step 3, when the experimental tooth sample is processed by the human tooth brushing model, the pressure of the electric toothbrush on the experimental tooth sample is 140-160 g.
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| EP1804708A1 (en) * | 2004-09-24 | 2007-07-11 | Align Technology, Inc. | Systems and methods for dental appliance compliance indication |
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| US6903813B2 (en) * | 2002-02-21 | 2005-06-07 | Jjl Technologies Llc | Miniaturized system and method for measuring optical characteristics |
| US20050271602A1 (en) * | 2004-06-02 | 2005-12-08 | Nebojsa Milanovich | Method for inhibiting chemical staining of teeth |
| CN202313795U (en) * | 2011-11-04 | 2012-07-11 | 大连恒艺信息科技有限公司 | Multi-regional dental digital color comparator |
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| WO2003092536A1 (en) * | 2002-05-01 | 2003-11-13 | Orametrix, Inc. | Three-dimensional virtual occlusal and interproximal contact detection |
| EP1804708A1 (en) * | 2004-09-24 | 2007-07-11 | Align Technology, Inc. | Systems and methods for dental appliance compliance indication |
| CN105662624A (en) * | 2016-03-31 | 2016-06-15 | 姚科 | Method and device for realizing color comparison of false teeth |
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