CN109369822A - A kind of extracting method of prebiotic bacterium exopolysaccharide - Google Patents

A kind of extracting method of prebiotic bacterium exopolysaccharide Download PDF

Info

Publication number
CN109369822A
CN109369822A CN201811485058.4A CN201811485058A CN109369822A CN 109369822 A CN109369822 A CN 109369822A CN 201811485058 A CN201811485058 A CN 201811485058A CN 109369822 A CN109369822 A CN 109369822A
Authority
CN
China
Prior art keywords
prebiotic bacterium
extracting method
polyamide
supernatant
bacterium exopolysaccharide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811485058.4A
Other languages
Chinese (zh)
Inventor
胡风庆
万姝含
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Liaoning University
Original Assignee
Liaoning University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Liaoning University filed Critical Liaoning University
Priority to CN201811485058.4A priority Critical patent/CN109369822A/en
Publication of CN109369822A publication Critical patent/CN109369822A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of extracting methods of prebiotic bacterium exopolysaccharide.The technical solution adopted is that: probiotics fermention liquid is centrifuged off precipitating thallus, 80-100 mesh polyamide is added and vibrates 2-10h, filter recycling polyamide, it takes filtrate decompression to be concentrated into the 20-80% of original volume, adjusts pH value to 5-9, add 95% ethyl alcohol of 1-5 times of volume, 4 DEG C of standing 2-16h, it is centrifuged the supernatant that inclines, precipitating is dissolved with appropriate distilled water, is freeze-dried and is saved after dialysis.The present invention is specified exocellular polysaccharide is extracted from probiotics fermention liquid during the method for removing protein and the technique of alcohol precipitation, can effectively improve the yield of prebiotic bacterium exopolysaccharide using method of the invention, obtain the exocellular polysaccharide for having certain antioxidant activity.

Description

A kind of extracting method of prebiotic bacterium exopolysaccharide
Technical field
The present invention relates to a kind of extracting methods of prebiotic bacterium exopolysaccharide, belong to microbiological art.
Background technique
Prebiotic bacterium exopolysaccharide refers to the cometabolism that some probiotics are secreted into outside cell wall during growth metabolism Product can be divided into two kinds according to the ability power of itself and cell wall-bound, a kind of to lose the ability with cell combination and penetrate into training It supports base and forms mucus, claim glue polysaccharide；Another kind is adhered to cell wall and forms pod membrane, claims capsular polysaccharide.In the natural environment, born of the same parents Exo polysaccharides usually play a part of protecting microorganism, it can to avoid cell drying and dehydrating, by bacteriophage dip dyeing, by antibiotic or Person's noxious material damage, protozoan injury, can with steady seepage pressure, sertoli cell structure, participate in cellular informatics transmitting and Cell composition etc..
Prebiotic bacterium exopolysaccharide is as a kind of safe natural products, because of its unique physicochemical property, more and more by Applied in food industry.It can improve rheological characteristic, emulsibility, tackness and the texture characteristic of dairy products and soya-bean milk products, It is set to become finer and smoother uniform, smooth dense, moreover it is possible to which raising bakes based food water-retaining property, improves loaf volume and pliability. In recent years, go deep into research, the physiological functions such as the anti-oxidant, antitumor of prebiotic bacterium exopolysaccharide, immunological regulation are also gradually Cause the concern of people.
Currently, the method for removing protein mostly uses trichloroacetic acid method in exocellular polysaccharide extraction process, method reaction is violent, easily The structure of polysaccharide is destroyed, and trichloroacetic acid itself has toxicity, the polysaccharide that this method obtains is unfavorable for functional study and application.
Since probiotics strain self character is different, in existing production method, yield of extracellular polysaccharide is from tens to several hundred It milligrams per liter differs, yield is lower, restricts its large-scale production, therefore how to improve yield and extracted amount is current to be badly in need of solving The problem of.Currently we are more to optimization culture based component and the research of fermentation condition raising yield of extracellular polysaccharide, and to optimization The research that exocellular polysaccharide is extracted from fermentation liquid is very few.
Summary of the invention
Aiming at the problem that prebiotic bacterium exopolysaccharide extracted amount, to improve probiotics extracellular the object of the present invention is to provide a kind of The preparation method of Polyose extraction amount.
The technical solution adopted by the present invention is that: a kind of preparation method of prebiotic bacterium exopolysaccharide includes the following steps:
1) remove thallus: by probiotics fermention liquid under the conditions of 4 DEG C, 10000r/min is centrifuged 10min, removes precipitating thallus, Retain supernatant；
2) removing protein: polyamide being added into supernatant obtained in step 1), is placed in shaking table oscillation 2-10h at room temperature, So that protein is adsorbed in polyamide, then filters removal polyamide；
3) it is concentrated: filtrate obtained by step 2) being concentrated into the 20-80% of original volume, obtains concentrate；
4) alcohol precipitation: adjusting the pH of concentrate, 95% ethyl alcohol is added, 4 DEG C of standings, centrifugation, incline supernatant, and precipitating is used appropriate It after distilled water dissolution, sets and dialyses in bag filter for 24 hours, freeze-drying saves.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, in step 2), by solid-to-liquid ratio, polyamide: supernatant= 40-200g:1L.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, in step 2), by solid-to-liquid ratio, polyamide: supernatant= 120g:1L.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, in step 2), the polyamide partial size is 80-100 Mesh.
Filtrate obtained by step 2) in step 3), is concentrated into original by a kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide The 20% of volume.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, in step 4), the additional amount of 95% ethyl alcohol is 1-5 times of concentrate.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, in step 4), the additional amount of 95% ethyl alcohol is 4 times of concentrate.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, in step 4), the time of the standing is 2-16h.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, in step 4), the pH is adjusted to 5-9.
A kind of extracting method of above-mentioned prebiotic bacterium exopolysaccharide, the probiotics are Lactobacillus rhamnosus.
The beneficial effects of the present invention are:
1. the present invention by optimization removing protein and alcohol precipitation process, show that prebiotic bacterium exopolysaccharide recovery rate is higher and economical suitable Removing protein and alcohol precipitation condition.
2. prebiotic bacterium exopolysaccharide prepared by the method for the present invention has antioxidation.
3. the present invention uses polyamide, the protein and other impurity in fermentation liquid are removed by suction-operated, react item Part is mild, nonhazardous, and polyamide can recycle, and have the characteristics that energy conservation and environmental protection.
Detailed description of the invention
Fig. 1 is the Lactobacillus rhamnosus exocellular polysaccharide that the present invention obtains, and is in pale yellow powder.
Fig. 2 is scavenging effect of the Lactobacillus rhamnosus exocellular polysaccharide to DPPH free radical.
Fig. 3 is scavenging effect of the Lactobacillus rhamnosus exocellular polysaccharide to OH free radical.
Specific embodiment
The determination of 1 Lactobacillus rhamnosus fermentation liquid removing protein condition of embodiment
Method: 1) activating: Lactobacillus rhamnosus activated from plate, and picking single bacterium drops down onto 10mlMRS culture solution, and 37 DEG C, 180rpm shaking table culture 12h obtains Lactobacillus rhamnosus fermentation seed liquid.
2) it ferments: Lactobacillus rhamnosus fermentation seed liquid is inoculated in MRS fluid nutrient medium with 2% inoculum concentration, adjust Saving initial pH value is 6.5, and at 37 DEG C, revolving speed 100r/min, culture for 24 hours, obtains fermentation liquid.The composition of MRS fluid nutrient medium Are as follows: galactooligosaccharide 1%, soy peptone 2%, phosphate 0.2%, yeast powder 0.5%, citric acid hydrogen diamine 0.2%, acetic acid Sodium 0.5%, magnesium sulfate 0.058%, manganese sulfate 0.025%, remaining is distilled water.
3) remove thallus: by fermentation liquid obtained in step 2) under the conditions of 4 DEG C, it is heavy that 10000r/min is centrifuged 10min removal Shallow lake thallus retains supernatant.
4) removing protein: being added (80-100) purpose polyamide in the supernatant obtained in step 3), shaking table shakes at room temperature It shakes, filters the polyamide for removing adsorbed proteins.
5) protein clearance rate measures: with the concentration of remaining protein in Coomassie Brilliant Blue measurement fermentation liquid, calculating egg The clearance rate of white matter.
(1) influence of different removing protein times
Method is same as above.By solid-to-liquid ratio, polyamide: polyamide is added into supernatant for supernatant=80g:1L, shakes the time Such as table 1.As a result such as table 1:
Table 1
Seen from table 1, protein clearance rate is improved with the extension of shaking time, and when shaking 4h or more, protein is removed Rate variation is unobvious, comprehensively considers removing protein effect and time cost, selects 4h for optimal removing protein time, protein clearance rate Up to 56.8%.
(2) influence of different polyamide additive amount
Method is same as above.Polyamide is added in except the supernatant after thallus, additive amount such as table 2 shakes 6h.As a result such as table 2:
Table 2
As can be seen from Table 2, protein clearance rate is improved with the raising of polyamide additive amount, polyamide additive amount is greater than Clearance rate variation is unobvious when 120g/L, comprehensively considers removing protein effect and cost, selects 120g/L for the optimal addition of polyamide Amount, protein clearance rate is up to 59.9%.
It is obtained by embodiment 1, the best removing protein condition of Lactobacillus rhamnosus exocellular polysaccharide are as follows: press solid-to-liquid ratio, polyamide: Polyamide is added in supernatant=120g:1L ratio, and shaking table shakes 4h.
The determination of 2 Lactobacillus rhamnosus fermentation liquid of embodiment concentration condition
Method: 1) activating: Lactobacillus rhamnosus activated from plate, and picking single bacterium drops down onto 10mlMRS culture solution, and 37 DEG C, 180rpm shaking table culture 12h obtains Lactobacillus rhamnosus fermentation seed liquid.
2) it ferments: Lactobacillus rhamnosus fermentation seed liquid is inoculated in MRS fluid nutrient medium with 2% inoculum concentration, adjust Saving initial pH value is 6.5, and at 37 DEG C, revolving speed 100r/min, culture for 24 hours, obtains fermentation liquid.
The composition of MRS fluid nutrient medium are as follows: galactooligosaccharide 1%, soy peptone 2%, phosphate 0.2%, yeast powder 0.5%, citric acid hydrogen diamine 0.2%, sodium acetate 0.5%, magnesium sulfate 0.058%, manganese sulfate 0.025%, remaining is distilled water.
3) remove thallus: by fermentation liquid obtained in step 2) under the conditions of 4 DEG C, it is heavy that 10000r/min is centrifuged 10min removal Shallow lake thallus, obtains supernatant.
4) removing protein: press solid-to-liquid ratio, polyamide: it is poly- that (80-100) purpose is added into supernatant for supernatant=120g:1L Amide, shaking table shakes 4h at room temperature, filters the polyamide for removing adsorbed proteins.
5) it is concentrated: step 4) products therefrom being concentrated, concentrate is obtained.
6) alcohol precipitation: 95% ethyl alcohol of concentrate three times volume is added into concentrate, in 4 DEG C of refrigeration 12h.In 4 DEG C of conditions Lower 10000r/min is centrifuged 10min, removes supernatant, and precipitating is dissolved with quantitative distilled water to get exocellular polysaccharide solution.After freeze-drying Weighing, and calculate exocellular polysaccharide yield.
(1) influence of concentration volume score
Method is same as above.Fermentation liquid is after bactofugation body, removing protein, as table 3 is concentrated.As a result such as table 3:
Table 3
Seen from table 3, polysaccharide yield increases with the reduction of volume after concentration.But it will lead to alcohol when cycles of concentration is excessively high Heavy obtained polysaccharide color is deeper, and it is larger to consume energy.Comprehensively consider yield and economic factor, selection is concentrated into the 20% of original volume As optimal conditions, polysaccharide yield is up to 11.804g/L.
The determination of 3 Lactobacillus rhamnosus exocellular polysaccharide alcohol precipitation condition of embodiment
Method: 1) activating: Lactobacillus rhamnosus activated from plate, and picking single bacterium drops down onto 10mlMRS culture solution, and 37 DEG C, 180rpm shaking table culture 12h obtains Lactobacillus rhamnosus fermentation seed liquid.
2) it ferments: Lactobacillus rhamnosus fermentation seed liquid is inoculated in MRS fluid nutrient medium with 2% inoculum concentration, adjust Saving initial pH value is 6.5, and at 37 DEG C, revolving speed 100r/min, culture for 24 hours, obtains fermentation liquid.
The composition of MRS fluid nutrient medium are as follows: galactooligosaccharide 1%, soy peptone 2%, phosphate 0.2%, yeast powder 0.5%, citric acid hydrogen diamine 0.2%, sodium acetate 0.5%, magnesium sulfate 0.058%, manganese sulfate 0.025%, remaining is distilled water.
3) remove thallus: 10000r/min is centrifuged 10min removal precipitating thallus, obtains supernatant.
4) removing protein: press solid-to-liquid ratio, polyamide: it is poly- that (80-100) purpose is added into supernatant for supernatant=120g:1L Amide, shaking table shakes 4h at room temperature, filters the polyamide for removing adsorbed proteins.
5) it is concentrated: step 4) products therefrom being concentrated into the 40% of original volume, obtains concentrate.
6) alcohol precipitation: adjusting concentrate pH, and 95% ethyl alcohol is added, and refrigerates in 4 DEG C.10000r/min is centrifuged under the conditions of 4 DEG C 10min, removes supernatant, and precipitating is dissolved with quantitative distilled water to get exocellular polysaccharide solution.It weighs, and calculates extracellular after freeze-drying Polysaccharide yield.
(1) influence of difference pH
Method is same as above.After fermentation liquid bactofugation body, removing protein, concentration, if table 4 adjusts pH value, 3 times of bodies of concentrate are added 95% long-pending ethyl alcohol stands 8h.As a result such as table 4:
Table 4
By table 4 as it can be seen that polysaccharide yield is with the raising first increases and then decreases of pH, the polysaccharide that alcohol precipitation obtains when pH=7 is most, Polysaccharide yield is up to 6.453g/L at this time.
(2) influence of different ethyl alcohol addition multiples
Method is same as above.After fermentation liquid bactofugation body, removing protein, concentration, pH to 6 is adjusted, if 95% ethyl alcohol is added in table 5, Stand 8h.As a result such as table 5:
Table 5
By table 5 as it can be seen that polysaccharide yield is with the increase first increases and then decreases of ethyl alcohol addition multiple, it is 4 times that ethyl alcohol, which adds multiple, When polysaccharide yield highest, be 6.344g/L.
(2) influence of different alcohol precipitation times
Method is same as above.After fermentation liquid bactofugation body, removing protein, pH to 6 is adjusted, 3 times of 95% second of volume of concentrate are added Alcohol, time of repose such as table 6.As a result such as table 6:
Table 6
By table 6 as it can be seen that polysaccharide yield is improved with the extension of alcohol precipitation time, and growth rate gradually slows down, and comprehensively considers Yield and time cost select 12h for the optimal alcohol precipitation time, and polysaccharide yield is 6.253g/L at this time.
It is obtained by embodiment 2 and embodiment 3, the optimum recovery condition of prebiotic bacterium exopolysaccharide are as follows: be concentrated into fermentation liquid The 20% of original volume, adjusting pH is 7, adds 95% ethyl alcohol of 4 times of volumes, 4 DEG C of standing 12h.
The verifying of 4 Lactobacillus rhamnosus exocellular polysaccharide extracted amount of embodiment
Method: 1) activating: Lactobacillus rhamnosus activated from plate, and picking single bacterium drops down onto 10mlMRS culture solution, and 37 DEG C, 180rpm shaking table culture 12h obtains Lactobacillus rhamnosus fermentation seed liquid.
2) it ferments: Lactobacillus rhamnosus fermentation seed liquid is inoculated in MRS fluid nutrient medium with 2% inoculum concentration, adjust Saving initial pH value is 6.5, and at 37 DEG C, revolving speed 100r/min, culture for 24 hours, obtains fermentation liquid.
The composition of MRS fluid nutrient medium are as follows: galactooligosaccharide 1%, soy peptone 2%, phosphate 0.2%, yeast powder 0.5%, citric acid hydrogen diamine 0.2%, sodium acetate 0.5%, magnesium sulfate 0.058%, manganese sulfate 0.025%, remaining is distilled water.
3) remove thallus: 10000r/min is centrifuged 10min removal precipitating thallus, obtains supernatant.
4) removing protein: press solid-to-liquid ratio, polyamide: it is poly- that (80-100) purpose is added into supernatant for supernatant=120g:1L Amide, shaking table shakes 4h at room temperature, filters the polyamide for removing adsorbed proteins.
5) it is concentrated: step 4) products therefrom being concentrated into the 20% of original volume, obtains concentrate.
6) alcohol precipitation: concentrate pH to 7 is adjusted, 95% ethyl alcohol of 4 times of volumes is added, in 4 DEG C of refrigeration 12h.Under the conditions of 4 DEG C 10000r/min is centrifuged 10min, removes supernatant, and precipitating is dissolved with quantitative distilled water to get exocellular polysaccharide solution.Claim after freeze-drying Weight, and calculate exocellular polysaccharide yield.
Experimental result: under above-mentioned optimal conditions, the yield of Lactobacillus rhamnosus exocellular polysaccharide is 15.52g/L.
5 Lactobacillus rhamnosus exocellular polysaccharide of embodiment is removing the application in free radical
The preparation of sample:
1) it activating: Lactobacillus rhamnosus is activated from plate, picking single bacterium drops down onto 10mlMRS culture solution, and 37 DEG C, 180rpm shaking table culture 12h, obtains Lactobacillus rhamnosus fermentation seed liquid.
2) it ferments: Lactobacillus rhamnosus fermentation seed liquid is inoculated in MRS fluid nutrient medium with 3% inoculum concentration, adjust Saving initial pH value is 6.5, at 28 DEG C, cultivates 10h, obtains fermentation liquid.
The composition of the MRS fluid nutrient medium are as follows: by weight percentage, galactooligosaccharide 1%, soy peptone 2%, Dipotassium hydrogen phosphate 0.2%, yeast powder 0.5%, citric acid hydrogen diamine 0.2%, sodium acetate 0.5%, magnesium sulfate 0.058%, sulfuric acid Manganese 0.025%, remaining is distilled water；The carbon-nitrogen ratio of the MRS fluid nutrient medium of composition is 1:2.
3) remove thallus: by fermentation liquid obtained in step 2) under the conditions of 4 DEG C, 10000r/min is centrifuged 10min, and it is heavy to remove Shallow lake thallus retains supernatant.
4) removing protein: press solid-to-liquid ratio, polyamide: it is poly- that (80-100) purpose is added into supernatant for supernatant=120g:1L Amide, shaking table shakes 4h at room temperature, filters the polyamide for removing adsorbed proteins.
5) it is concentrated: step 4) products therefrom being concentrated into the 20% of original volume, obtains concentrate.
6) alcohol precipitation: adjusting the pH to 7 of concentrate, 95% ethyl alcohol of 4 times of volumes of concentrate is added, in 4 DEG C of refrigeration 12h.In 4 10000r/min is centrifuged 10min under the conditions of DEG C, removes supernatant, precipitating is with freeze-drying after the dissolution of quantitative distilled water to get rhamnose Lactobacillus exocellular polysaccharide.
Radicals scavenging experiment:
1) DPPH radicals scavenging is tested: taking the OD of 2mL sample+2mL 0.2mM DPPH517For Ai；2mL sample+2mL The OD of dehydrated alcohol517For Aj；The OD of 2mL 0.2mM DPPH+2mL dehydrated alcohol517For Ac.Control group is with ascorbic acid generation For sample.Every group of experiment is in triplicate.
Calculate clearance rate formula:
DPPH free radical scavenging activity (%)=[1- (Ai-Aj)/Ac] × 100%
2) OH radicals scavenging is tested: taking 2mL sample+2mL 3mmol/L salicylic acid-ethanol solution+0.6mL 8mmol/L FeSO4+0.5mL 20mmol/L H2O2, flowing water is cooling after 37 DEG C of water-bath 30min, and 0.9mL distilled water, 3000r/ is added Min is centrifuged 10min, and it is A that absorbance is measured at 510nm1；Sample is substituted with distilled water, is ibid operated, is measured at 510nm Absorbance is A0.Control group replaces sample with ascorbic acid.Every group of experiment is in triplicate.
OH free radical scavenging activity (%)=(1-A1/A0) × 100%
Experimental result:
1. as shown in Fig. 2, Lactobacillus rhamnosus exocellular polysaccharide is to the elimination efficiency of DPPH free radical as sample concentration mentions It is high and improve.When content is 2.5mg/mL, efficiency is inhibited to reach 13.8%, when concentration reaches 20g/mL, efficiency is inhibited to reach To 85.24%.
2. as shown in figure 3, Lactobacillus rhamnosus exocellular polysaccharide is to the elimination efficiency of OH free radical as sample concentration improves And it improves.When content is 2.5mg/mL, efficiency is inhibited to reach 2.0%, when concentration reaches 20g/mL, efficiency is inhibited to reach 84.93%.

Claims (10)

1. a kind of extracting method of prebiotic bacterium exopolysaccharide, which comprises the steps of:
1) remove thallus: by probiotics fermention liquid under the conditions of 4 DEG C, 10000r/min is centrifuged 10min, removes precipitating thallus, retains Supernatant；
2) removing protein: polyamide being added into supernatant obtained in step 1), is placed in shaking table oscillation 2-10h at room temperature, makes egg White matter is adsorbed in polyamide, then filters removal polyamide；
3) it is concentrated: filtrate obtained by step 2) being concentrated into the 20-80% of original volume, obtains concentrate；
4) alcohol precipitation: adjusting the pH of concentrate, and 95% ethyl alcohol is added, and 4 DEG C of standings, centrifugation, incline supernatant, the appropriate distillation of precipitating It after water dissolution, sets and dialyses in bag filter for 24 hours, freeze-drying saves.
2. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 1, which is characterized in that in step 2), press Solid-to-liquid ratio, polyamide: supernatant=40-200g:1L.
3. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 2, which is characterized in that in step 2), press Solid-to-liquid ratio, polyamide: supernatant=120g:1L.
4. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 1, which is characterized in that in step 2), institute The polyamide partial size stated is 80-100 mesh.
5. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 1, which is characterized in that, will in step 3) Filtrate obtained by step 2) is concentrated into the 20% of original volume.
6. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 1, which is characterized in that in step 4), institute The additional amount for 95% ethyl alcohol stated is 1-5 times of concentrate.
7. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 6, which is characterized in that in step 4), institute The additional amount for 95% ethyl alcohol stated is 4 times of concentrate.
8. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 1, which is characterized in that in step 4), institute The time for the standing stated is 2-16h.
9. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 1, which is characterized in that in step 4), institute The pH stated is adjusted to 5-9.
10. a kind of extracting method of prebiotic bacterium exopolysaccharide according to claim 1, which is characterized in that described is prebiotic Bacterium is Lactobacillus rhamnosus.
CN201811485058.4A 2018-12-06 2018-12-06 A kind of extracting method of prebiotic bacterium exopolysaccharide Pending CN109369822A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811485058.4A CN109369822A (en) 2018-12-06 2018-12-06 A kind of extracting method of prebiotic bacterium exopolysaccharide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811485058.4A CN109369822A (en) 2018-12-06 2018-12-06 A kind of extracting method of prebiotic bacterium exopolysaccharide

Publications (1)

Publication Number Publication Date
CN109369822A true CN109369822A (en) 2019-02-22

Family

ID=65375926

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811485058.4A Pending CN109369822A (en) 2018-12-06 2018-12-06 A kind of extracting method of prebiotic bacterium exopolysaccharide

Country Status (1)

Country Link
CN (1) CN109369822A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110840781A (en) * 2019-11-15 2020-02-28 华熙生物科技股份有限公司 Preparation method of tremella spore fermentation extract and product thereof
CN113372463A (en) * 2021-07-29 2021-09-10 江南大学 Method for extracting probiotic functional sugar from distilled rice wine distillation residual liquid
CN115232226A (en) * 2022-08-26 2022-10-25 南京轩凯生物科技股份有限公司 Extraction method of Pantoea alhagi extracellular polysaccharide

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080070805A1 (en) * 2006-09-20 2008-03-20 Halliburton Energy Services, Inc. Drill-in fluids and associated methods
CN105219817A (en) * 2015-11-02 2016-01-06 辽宁大学 A kind of preparation method and application of Exopolysaccharides Produced by Lactic Acid Bacteria

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080070805A1 (en) * 2006-09-20 2008-03-20 Halliburton Energy Services, Inc. Drill-in fluids and associated methods
CN105219817A (en) * 2015-11-02 2016-01-06 辽宁大学 A kind of preparation method and application of Exopolysaccharides Produced by Lactic Acid Bacteria

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
孟利 等: "聚酰胺柱层析法去除西藏灵菇胞外多糖发酵液中蛋白质的研究", 《食品工业科技》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110840781A (en) * 2019-11-15 2020-02-28 华熙生物科技股份有限公司 Preparation method of tremella spore fermentation extract and product thereof
CN110840781B (en) * 2019-11-15 2022-12-30 华熙生物科技股份有限公司 Preparation method of tremella spore fermentation extract and product thereof
CN113372463A (en) * 2021-07-29 2021-09-10 江南大学 Method for extracting probiotic functional sugar from distilled rice wine distillation residual liquid
CN113372463B (en) * 2021-07-29 2022-03-11 江南大学 Method for extracting probiotic functional sugar from distilled rice wine distillation residual liquid
CN115232226A (en) * 2022-08-26 2022-10-25 南京轩凯生物科技股份有限公司 Extraction method of Pantoea alhagi extracellular polysaccharide
CN115232226B (en) * 2022-08-26 2024-03-15 南京轩凯生物科技股份有限公司 Extraction method of external polysaccharide of Pantoea alhagi

Similar Documents

Publication Publication Date Title
CN109369822A (en) A kind of extracting method of prebiotic bacterium exopolysaccharide
CN109182171B (en) Mutagenic strain for high yield of gamma-aminobutyric acid and biological preparation thereof
CN111019860B (en) Pediococcus acidilactici for reducing biogenic amine and application thereof
CN103146599B (en) Lactobacillus plantarum producing high yield gamma-aminobutyric acid and application of lactobacillus plantarum
CN102839135B (en) Lactobacillus fermentum capable of producing gamma-aminobutyric acid with high yield and application thereof
CN104293680B (en) The preparation method and product of red yeast rice strain, high active ingredient functional Monascus powder
CN106434421B (en) One plant of epsilon-polylysine superior strain and production epsilon-polylysine method
US20140187829A1 (en) Method of Producing Natural B Carotene By Fermentation And Use Thereof
CN110129216B (en) Bacillus subtilis mutant strain suitable for producing gamma-polyglutamic acid by solid fermentation and culture method thereof
CN104450826A (en) Coriolus versicolor polysaccharide extracts and preparation method and application thereof
CN109207544A (en) A kind of preparation method of chlorella antioxidation polypeptide
CN111493292A (en) A method for preparing refined paste from marine organism and/or marine product processing waste
CN105039484A (en) High-yield carotinoid and copper fermentation culture method by using Rhodotorula benthica
CN105104610A (en) Preparation method and application of tea fermentation bacterium yeast
CN104928199B (en) The copper-rich ocean rhodotorula and its fermentation culture method of one plant of High Yield of Carotenoid
CN111328634A (en) Preparation method of velvet antler mushroom liquid strain culture medium
CN109258296B (en) Semi-continuous submerged fermentation process for high-yield auricularia auricula polysaccharide
CN109112175B (en) Method for constructing microbial co-culture system to produce pleurotus tuber-regium exopolysaccharide at high yield
CN106635834B (en) The Thelephora ganbajun mycelium zinc polysaccharide and application that one plant of wizened bacteria strain and its fermentation obtain
CN105520107A (en) New vegetable fermentation production method
CN102251002A (en) Method for preparing antioxidant polypeptide by solid fermentation of acanthogobius hasta muscle protein
CN112481341B (en) Preparation method and application of selenium absorption enhancing active peptide
CN115025033A (en) Mulberry leaf fermented composition, preparation method and application
CN104649782A (en) Agrocybe cylindracea liquid strain culture solution and culture method of agrocybe cylindracea liquid strain
CN114668055A (en) Auxiliary material composition for tea fermentation and fermentation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20190222