CN109337861B - CHO cell serum-free medium supporting high expression of product - Google Patents
CHO cell serum-free medium supporting high expression of product Download PDFInfo
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Abstract
The invention discloses a CHO cell serum-free medium, which mainly comprises amino acid, inorganic salt components, vitamins, trace elements, yeast hydrolysate, albumin and the like. The CHO cell serum-free culture medium does not contain any serum component or animal-derived component, and the affinity of the CHO cell serum-free culture medium is increased compared with other serum-free culture media. Cells can be directly inoculated into the serum-free culture medium from the serum culture medium or other serum-free culture media, and grow normally, so that a complicated acclimation process is avoided. The invention improves the growth density and the survival rate of the CHO cells, increases the tolerance performance of the cells in the CHO cells, prolongs the maintenance time of a growth platform, and most importantly, improves the capability of the CHO engineering cells for expressing foreign proteins in the CHO cells and greatly improves the yield of products.
Description
Technical Field
The invention relates to a serum-free culture medium for CHO cell in vitro culture, belonging to the technical field of cell culture.
Background
The cell culture technology is also called cell cloning technology, and means that under the condition of in vitro, by adding specific nutrient substances and controlling environmental temperature, pH value osmotic pressure and the like, cells can keep active and normally grow in a proper vessel. According to different adherence modes of cells in a vessel, the culture mode can be divided into adherence culture and suspension culture. The cell culture medium is a source for the cells to absorb nutrients and is the key point for successful cell culture. Cell culture media can be divided into natural media and synthetic media according to their sources. Natural media consists only of naturally occurring biological fluids. The natural culture medium is very useful and convenient, and is suitable for culturing various animal cells. However, the main disadvantage of using natural media is poor reproducibility due to lack of knowledge of the exact composition of these natural media. The artificial synthetic culture medium is a culture medium which is formed by artificially adding nutrient substances with specific components and carrying out proper proportioning and is suitable for cell growth. As synthetic media continues to develop, the classification of synthetic media is gradually refined, and the synthetic media can be classified into serum-containing media, serum-containing substitute media and serum-free media. The use of serum-free medium improves the repeatability of cell culture, avoids the influence caused by the difference between serum batches, and reduces the risk of virus, fungus, mycoplasma and other microbial contamination caused by serum. Meanwhile, the obtained cell product is easy to purify. The advantages enable the serum-free culture medium to be more and more widely applied.
Chinese Hamster Ovary (CHO) cells are important engineering cells, exogenous genes are easy to express in the CHO cells, and products are secretory and easy to separate and purify. Therefore, the recombinant DNA is already an important host cell in cell engineering. In the current market, all large-brand culture medium companies including imported and domestic culture medium companies have CHO serum-free culture medium products, but the price is relatively high, the use cost is high, and the serum-free culture medium is difficult to be applied on a large scale. For engineering CHO cells in production applications, it is critical to obtain high expression levels under normal growth conditions. The invention relates to a serum-free culture medium integrating cell growth and product expression performance, which is a practical requirement of the market.
Disclosure of Invention
Aiming at the prior art, the invention provides a CHO cell serum-free culture medium supporting high expression of a product, and the serum-free culture of cells can be completely realized. By adopting the serum-free medium for culture, the CHO cells with high density, fast growth and high expression level can be obtained. The invention can shorten the logarithmic phase of cell growth and prolong the plateau phase, and is beneficial to the generation of products, thereby obtaining more target products and improving the production efficiency.
The invention is realized by the following technical scheme:
a CHO cell serum-free medium supporting high expression of a product is composed of the following components in concentration:
addition concentration of substance name (mg/L)
Amino acids
Glycine 150-
L-alanine 5-50
L-arginine hydrochloride 200-
L-asparagine 300-1500-
L-aspartic acid 110-320
L-cysteine hydrochloride 50-200
L-glutamic acid 135-250-
L-histidine hydrochloride 160-
L-isoleucine 125-
L-leucine 50-450
L-lysine hydrochloride 55-280
L-methionine 45-375
L-phenylalanine 30-160
L-proline 56-90
L-serine 220-355
L-threonine 120-
L-Tryptophan 110-230
L-tyrosine disodium 250-450-
L-valine 250-460
L-cystine 100-300-
Hydroxyproline 2.4-3.5
Vitamin preparation
Choline chloride 130-
5-8 parts of calcium D-pantothenate
Folic acid 1.7-3.5
Nicotinamide 3.7-6.6
Pyridoxine hydrochloride 6.5-9.5
Riboflavin 0.5-2.3
Thiamine hydrochloride 3.8-5.6
Vitamin B121-10
Inositol 10-100
Lipoic acid 5-10
Biotin 0.003-0.03
Retinol 0.005-0.5
Para aminobenzoic acid 0.008-0.015
Inorganic salt
100-200 calcium chloride dihydrate
Sodium bicarbonate 1000-
Magnesium sulfate heptahydrate 50-150
500-950 of potassium chloride
Sodium chloride 2500-
Disodium hydrogen phosphate heptahydrate 100-300
Trace elements
Ammonium metavanadate 0.00015-0.00036
0.002-0.05% of sodium metasilicate
Manganese chloride tetrahydrate 0.00002-0.0001
Cobalt chloride hexahydrate 0.001-0.002
Aluminum chloride hexahydrate 0.0001-0.0005
Chromium chloride hexahydrate of 0.00001-0.0001
Nickel chloride hexahydrate 0.00001-0.0001
Zirconium oxychloride octahydrate 0.00001-0.0001
Germanium dioxide 0.00003-0.0003
0.1-1 part of zinc sulfate heptahydrate
Ferrous sulfate heptahydrate 0.25-1
Copper sulfate pentahydrate 0.002-0.02
Sodium selenite 0.0005-0.005
Carbohydrates and other ingredients
Linoleic acid 0.02-0.2
Linolenic acid 0.005-0.05
Lecithin 0.5-5
Stearic acid 0.01-0.025
Palmitoleic acid 0.003-0.009
Glucose 3000-
50-200 parts of sodium pyruvate
Yeast hydrolysate 200-
Putrescine hydrochloride 0.2-2
Recombinant human insulin 1-10
Pluronic 68500-
Taurine 100-
Ascorbic acid 50-200
Dextran sulfate 10-50
Hydrocortisone 0.1-0.5
Ethanolamine 0.25-0.75
Thymine 0.08-0.8
Ferric citrate 2000-10000
Recombinant human serum albumin 0.2-0.5
Tween-801-5
The balance being water.
The preparation method of the serum-free culture medium comprises the following steps: taking the components except water, classifying and dissolving the components according to respective dissolution characteristics, mixing, adding water to enable the final concentration of each component to be as above, adjusting the pH value to 7.0-7.4, and adjusting the osmotic pressure to 300-350 mOsm/kg, so as to obtain the filter element, filtering by an industrial filter element, and packaging under the protection of nitrogen (to avoid oxidation of unstable components).
The CHO cell serum-free culture medium supporting high expression of the product does not contain any serum component, avoids potential pollution and risk, solves the problem of biological safety, and simultaneously provides convenience for downstream purification.
Drawings
FIG. 1: CHO cells were subcultured continuously from a commercially available medium into the medium of example 3, and after inoculation, the cells were subcultured on days 4, 6 and 8, respectively, and were subcultured continuously 4 times, whereby the cells showed stable growth.
FIG. 2: CHO cells growth pattern of CHO cells in the culture medium of example 3 observed under a microscope.
FIG. 3: growth curves and corresponding cell viability for batch cultures of CHO cells in the medium of example 3.
FIG. 4: CHO cells were cultured in batch in the medium of example 3 to collect the results of measurement of the expression level of the antibody.
Detailed Description
The present invention will be further described with reference to the following examples.
In the invention, the raw materials are all imported cell culture grade reagents and are stored according to related requirements. The following experimental procedures are routine experimental procedures unless otherwise specified. The features of the present invention are described below in conjunction with the appended drawings. It should be understood that the following examples are only illustrative of the present invention and are not intended to limit the scope of the present invention.
EXAMPLE 1 preparation of CHO cell serum-free Medium
The concentrations of the raw materials are as follows:
addition concentration of substance name (mg/L)
Amino acids
Glycine 650
L-alanine 46
L-arginine hydrochloride 324
L-asparagine 850
L-aspartic acid 240
L-cysteine hydrochloride 118
L-glutamic acid 187
L-histidine hydrochloride 235
L-isoleucine 220
L-leucine 430
L-lysine hydrochloride 166
L-methionine 207
L-phenylalanine 122
L-proline 74.5
L-serine 256
L-threonine 156
L-Tryptophan 153.3
L-tyrosine disodium 306.8
L-valine 323
L-cystine 185
Hydroxyproline 2.79
Vitamin preparation
Choline chloride 155.5
Calcium D-pantothenate 6.21
Folic acid 1.97
Nicotinamide 4.57
Pyridoxine hydrochloride 9.12
Riboflavin 1.07
Thiamine hydrochloride 4.22
Vitamin B123.6
Inositol 79
Lipoic acid 8.83
Biotin 0.011
Retinol 0.026
Para aminobenzoic acid 0.01
Inorganic salt
Sodium bicarbonate 3700
Magnesium sulfate heptahydrate 77
Sodium chloride 4500
Disodium hydrogen phosphate heptahydrate 250
Trace elements
Ammonium metavanadate 0.00022
Sodium metasilicate 0.0025
Manganese chloride tetrahydrate 0.00008
Cobalt chloride hexahydrate 0.0011
Aluminum chloride hexahydrate 0.0003
Chromium chloride hexahydrate 0.00005
Nickel chloride hexahydrate 0.00005
Zirconium oxychloride octahydrate 0.00005
Germanium dioxide 0.00005
Heptahydrate zinc sulfate 0.9
Ferrous sulfate heptahydrate 0.95
Blue vitriod 0.0135
Sodium selenite 0.00179
Carbohydrates and other ingredients
Linoleic acid 0.098
Linolenic acid 0.015
Lecithin 3.4
Stearic acid 0.0183
Palmitoleic acid 0.007
Glucose 5000
Sodium pyruvate 170
Yeast hydrolysate 550
Putrescine hydrochloride 0.49
Recombinant human insulin 5
Pluronic 681000
Ascorbic acid 110
Dextran sulfate 25
Hydrocortisone 0.4
Ethanolamine 0.5
Thymine 0.54
Recombinant human serum albumin 0.3
Tween-801.5
The balance being water.
The preparation method comprises the following steps: taking the components except water, classifying and dissolving the components according to respective dissolution characteristics, mixing, adding water to enable the final concentration of each component to be as above, adjusting the pH value to 7.0-7.4, and adjusting the osmotic pressure to 300-350 mOsm/kg, so as to obtain the filter element, filtering by an industrial filter element, and packaging under the protection of nitrogen (to avoid oxidation of unstable components).
EXAMPLE 2 preparation of CHO cell serum-free Medium
The concentrations of the raw materials are as follows:
addition concentration of substance name (mg/L)
Amino acids
L-alanine 30
L-arginine hydrochloride 500
L-asparagine 505
L-aspartic acid 240
L-cysteine hydrochloride 130
L-glutamic acid 155
L-histidine hydrochloride 290
L-isoleucine 450
L-leucine 125
L-lysine hydrochloride 250
L-methionine 125
L-phenylalanine 60
L-proline 60
L-serine 350
L-threonine 300
L-Tryptophan 215
L-tyrosine disodium 400
L-valine 400
L-cystine 252
Hydroxyproline 3.3
Vitamin preparation
Choline chloride 215
D-calcium pantothenate 7.5
Folic acid 3.2
Nicotinamide 5.8
Pyridoxine hydrochloride 7.5
Riboflavin 2.1
Thiamine hydrochloride 5.0
Vitamin B128.2
Inositol 50
Lipoic acid 6.2
Biotin 0.02
Retinol 0.3
Para aminobenzoic acid 0.013
Inorganic salt
Calcium chloride dihydrate 180
Sodium bicarbonate 3700
Magnesium sulfate heptahydrate 120
Sodium chloride 4500
Disodium hydrogen phosphate heptahydrate 250
Trace elements
Ammonium metavanadate 0.0003
Sodium metasilicate 0.03
Manganese chloride tetrahydrate 0.00005
Cobalt chloride hexahydrate 0.0015
Aluminum chloride hexahydrate 0.0003
Chromium chloride hexahydrate 0.00005
Nickel chloride hexahydrate 0.00005
Zirconium oxychloride octahydrate 0.00005
Germanium dioxide 0.00005
0.2 of zinc sulfate heptahydrate
Ferrous sulfate heptahydrate 0.5
Copper sulfate pentahydrate 0.01
Sodium selenite 0.003
Carbohydrates and other ingredients
Linoleic acid 0.15
Linolenic acid 0.03
Lecithin 1.5
Stearic acid 0.012
Palmitoleic acid 0.004
Glucose 4500
Yeast hydrolysate 850
Putrescine hydrochloride 1.5
Recombinant human insulin 8
Pluronic 681500
Dextran sulfate 50
Hydrocortisone 0.2
Ethanolamine 0.6
Thymine 0.2
Ferric citrate 5000
Recombinant human serum albumin 0.4
Tween-801.5
The balance being water.
The preparation method comprises the following steps: taking the components except water, classifying and dissolving the components according to respective dissolution characteristics, mixing, adding water to enable the final concentration of each component to be as above, adjusting the pH value to 7.0-7.4, and adjusting the osmotic pressure to 300-350 mOsm/kg, so as to obtain the filter element, filtering by an industrial filter element, and packaging under the protection of nitrogen (to avoid oxidation of unstable components).
EXAMPLE 3 preparation of CHO cell serum-free Medium
The concentrations of the raw materials are as follows:
addition concentration of substance name (mg/L)
Amino acids
Glycine 650
L-alanine 30
L-arginine hydrochloride 300
L-asparagine 1000
L-aspartic acid 150
L-cysteine hydrochloride 150
L-glutamic acid 200
L-histidine hydrochloride 200
L-isoleucine 320
L-leucine 430
L-lysine hydrochloride 185
L-methionine 200
L-phenylalanine 150
L-proline 60
L-serine 285
L-threonine 250
L-Tryptophan 200
L-tyrosine disodium 423
L-valine 405
L-cystine 155
Hydroxyproline 3.0
Vitamin preparation
D-calcium pantothenate 5.8
Folic acid 3.3
Nicotinamide 4.2
Pyridoxine hydrochloride 8.5
Riboflavin 1.8
Thiamine hydrochloride 4.5
Vitamin B125.0
Inositol 25
Lipoic acid 8.5
Biotin 0.025
Retinol 0.3
Para aminobenzoic acid 0.01
Inorganic salt
Sodium bicarbonate 3700
Magnesium sulfate heptahydrate 77
Potassium chloride 900
Sodium chloride 4500
Disodium hydrogen phosphate heptahydrate 250
Trace elements
Ammonium metavanadate 0.00031
Sodium metasilicate 0.045
Manganese chloride tetrahydrate 0.00008
Cobalt chloride hexahydrate 0.0015
Aluminum chloride hexahydrate 0.0004
Chromium chloride hexahydrate 0.00003
Nickel chloride hexahydrate 0.00003
Zirconium oxychloride octahydrate 0.00003
Germanium dioxide 0.00003
0.5% zinc sulfate heptahydrate
Ferrous sulfate heptahydrate 0.8
Copper sulfate pentahydrate 0.015
Sodium selenite 0.004
Carbohydrates and other ingredients
Linoleic acid 0.15
Linolenic acid 0.035
Lecithin 1.5
Stearic acid 0.021
Palmitoleic acid 0.005
Glucose 5000
Yeast hydrolysate 800
Putrescine hydrochloride 1.5
Recombinant human insulin 8
Pluronic 681500
Taurine 180
Dextran sulfate 30
Hydrocortisone 0.3
Ethanolamine 0.55
Thymine 0.3
Ferric citrate 3000
Recombinant human serum albumin 0.4
Tween-803.5
The balance being water.
The preparation method comprises the following steps: taking the components except water, classifying and dissolving the components according to respective dissolution characteristics, mixing, adding water to enable the final concentration of each component to be as above, adjusting the pH value to 7.0-7.4, and adjusting the osmotic pressure to 300-350 mOsm/kg, so as to obtain the filter element, filtering by an industrial filter element, and packaging under the protection of nitrogen (to avoid oxidation of unstable components).
Experiment of
The CHO cell serum-free medium prepared in example 3 was used for cell culture. The specific method is to culture CHO cells stably in commercial culture medium at 0.5e6 cells/ml were inoculated into the finished media of the invention. The culture was carried out in 125 mL shake flasks, the inoculation volume being 30 mL. The culture conditions were 5% CO2 and the temperature wasThe rotational speed of the shaker at 37 ℃ is 110 r/min. After inoculation, samples were continuously taken every day for cell counting, and the viability of the cells was calculated by trypan blue staining.
Results
The suspension CHO cells have a good growth state in the serum-free culture medium, and the cells are singly dispersed and suspended without obvious cell agglomeration. The cell viability is high, the viability can be maintained to be more than 90% before the platform period through batch culture, and the continuous culture performance is stable. Therefore, in the culture medium, the ability of the cells to express the foreign protein is also obviously enhanced. The protein product can be harvested in 466mg/L per liter of culture in batch culture.
Although the specific embodiments of the present invention have been described with reference to the examples, the scope of the present invention is not limited thereto, and those skilled in the art will appreciate that various modifications and variations can be made without inventive effort by those skilled in the art based on the technical solution of the present invention.
Claims (5)
1. A CHO cell serum-free culture medium is characterized by comprising the following components in concentration:
the balance of water,
each concentration unit is mg/L.
2. The CHO cell serum-free medium according to claim 1, characterized by consisting of the following components in concentration:
the balance of water,
each concentration unit is mg/L.
3. The CHO cell serum-free medium according to claim 1, characterized by consisting of the following components in concentration:
the balance of water,
each concentration unit is mg/L.
4. The method for preparing a CHO cell serum-free medium according to any one of claims 1 to 3, wherein: the components are taken, classified and dissolved according to respective dissolution characteristics, then mixed and dissolved in deionized water, the pH value of the solution is adjusted to be 7.0-7.4, the osmotic pressure is 300-350 mOsm/kg, and the finished culture medium is obtained after filtration and sterilization.
5. Use of a CHO cell serum-free medium according to any one of claims 1 to 3 for culturing suspension CHO cells.
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| CN110592000A (en) * | 2019-08-13 | 2019-12-20 | 苏州易迈吉生物医药科技有限公司 | Serum-free medium supporting high-density suspension culture of BHK (baby hamster kidney) cells |
| CN110894487B (en) * | 2019-12-23 | 2022-07-19 | 新乡医学院 | Serum-free and protein-free CHO cell culture medium and preparation method and application thereof |
| CN113088480B (en) * | 2019-12-23 | 2022-10-11 | 信达生物制药(苏州)有限公司 | Culture medium for CHO cells and application thereof |
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| CN113151183A (en) * | 2021-04-21 | 2021-07-23 | 赵峻岭 | Culture medium additive for promoting protein expression and application thereof |
| CN113913368A (en) * | 2021-10-12 | 2022-01-11 | 浙江省立同德医院 | Serum-free medium suitable for CHO cell large-scale suspension amplification culture and preparation and application thereof |
| CN114525239A (en) * | 2022-03-03 | 2022-05-24 | 天津鸿宇泰生物科技有限公司 | Serum-free cell culture medium and preparation method thereof |
| CN115505561A (en) * | 2022-11-24 | 2022-12-23 | 天信和(苏州)生物科技有限公司 | Serum-free medium additive for CHO cells and application thereof |
| CN116515737B (en) * | 2023-06-28 | 2023-09-22 | 苏州依科赛生物科技股份有限公司 | HEK293 cell and CHO cell universal culture medium and application thereof |
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