CN109288910A - A kind of Chinese medicine cleaning solution and preparation method thereof for treating gynecology colpitis - Google Patents
A kind of Chinese medicine cleaning solution and preparation method thereof for treating gynecology colpitis Download PDFInfo
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Abstract
The present invention provides a kind of Chinese medicine cleaning solutions and preparation method thereof for treating gynecology colpitis, the cleaning solution is made of raw material from the following weight: 5~30 parts of honeysuckle, 1~20 part of kuh-seng, 1~10 part of radix scutellariae, 1~10 part of Cortex Phellodendri, the Chinese medicine cleaning solution for the treatment of gynecology colpitis of the present invention passes through all medicines share, the symptom of vaginitis can be improved rapidly, it has no toxic side effect, it is highly-safe, with good anti-inflammatory, antibacterial, itching-relieving action, the use of Western medicine can be substituted, the recurrent exerbation of this disease can be effectively reduced.
Description
Technical field
The present invention relates to pharmaceutical technology field, in particular to a kind of Chinese medicine cleaning solution for treating gynecology colpitis and its preparation
Method.
Background technique
Vaginitis is to lead to vulvovaginal symptom such as itch, cusalgia, one group of illness of stimulation and abnormal flow liquid.It is normal strong
The characteristics of health woman vagina is due to anatomical tissue has natural defense function to the intrusion of pathogen.Such as the closure of introitus, vagina
Front and rear wall is close to, hyperplasia and cells of superficial layer angling of the vaginal epithelial cell under the influence of estrogen, and vagina acid-base degree keeps flat
Weighing apparatus is suppressed the breeding of pathogen for adapting to alkalinity, and neck tube mucus is in alkalinity, when vagina natural defense function by
When destruction, pathogen is easy to invade, and leads to colpitis.
There are aerobic bacteria and anaerobic bacteria to reside in intravaginal under normal circumstances, forms normal vaginal flora.Any reason will
The ecological balance between vagina and flora is broken, can also formation condition pathogenic bacteria.It is clinically common to have: bacterial vaginosis BV, thought
Pearl bacterium property vaginitis, trichomonas vaginitis, senile vahinitis, infantile vaginitis.
Honeysuckle is known as clearing heat and detoxicating good medicine from ancient times.First is that antibacterial is universal, to staphylococcus aureus, hemolytic
Streptococcus, dysentery, typhoid fever, diplococcus, Diplococcus pneumopniae, green pus and influenza virus etc. have obvious inhibiting effect.Second is that having
The immune function of enhancing, can promote conversion, enhance the phagocytic function of leucocyte.Third is that it is anti-inflammatory, antipyretic, there is obvious inhibition to inflammation
Effect.
Kuh-seng, bitter are trembled with fear.There are heat-clearing and damp-drying drug, desinsection, the function of diuresis.For hot dysentery, hematochezia, jaundice urodialysis, red leukorrhea
Under, swelling of vulva pruritus vulvae, eczema, wet sore, pruitus, castor controls trichomonas vaginitis outside.Kuh-seng is to shigella dysenteriae, large intestine
Bacillus, proteus, beta streptococcus and staphylococcus aureus have apparent inhibiting effect.
Radix scutellariae, it is bitter, cold in nature, there are heat-clearing and damp-drying drug, purging fire for removing toxin, hemostasis, miscarriage prevention and other effects.The clinical antibiotic property ratio of radix scutellariae
The coptis is good, to shigella dysenteriae, typhoid bacillus, paratyphosum Bacterium, comma bacillus, Escherichia coli, proteus, Pseudomonas aeruginosa, Portugal
Grape coccus, hemolytic vessel used to hold grain at the imperial sacrifice coccus, Diplococcus pneumopniae, corynebacterium diphtheriae etc. have inhibiting effect, and do not develop drug resistance.
Cortex Phellodendri is the dry bark of rutaceae wampee.There is the effect of heat-clearing and damp-drying drug, purging intense heat, except steaming, detoxification sore treatment.
For damp-heat dysentery, jaundice and reddish urine, leukorrhagia pruritus vulvae, puckery pain of heat gonorrhea, tinea pedis impotence is preesed, hectic fever due to yin labor heat, night sweat, spermatorrhea, sore swollen toxin,
Eczema wet sore.Cortex Phellodendri has good antibacterial action to a variety of gram-positive bacteriums.
Application No. is 201210357058.2 Chinese patent disclose a kind of lotion of medication for treating vaginal discharge diseases of women and
Preparation method, the lotion of medication are prepared by the Chinese medicine material medicine of following weight proportion: 16-22 parts of Paris polyphylla, frutus cnidii
18-24 parts, 12-18 parts of Chinese prickly ash, 2-6 parts of menthol, 10-16 parts of cape jasmine, 3-9 parts of Gorgon fruit, 3-7 parts of the fleece-flower root, 4-10 parts of betel nut,
18-26 parts of oriental wormwood, 15-21 parts of kuh-seng, 6-10 parts of Radix Angelicae Pubescentis, 13-19 parts of the tuber of stemona, 8-12 parts of radix scrophulariae, 13-19 parts of honeysuckle, summer cypress
12-18 parts sub-, 6-10 parts of cortex dictamni, 14-20 parts of Golden Larch Bark, 11-17 parts of radix scutellariae, 6-12 parts of radix paeoniae rubra, 11-17 parts of folium artemisiae argyi, stone Chang
9-13 parts of Pu, 10-16 parts of Cortex Phellodendri, 10-18 parts of rhizoma atractylodis.
Application No. is 201510985104.7 Chinese patents to disclose a kind of lotion of compound flavescent sophora root for treating gynecological disease
And preparation method thereof, it is white with 20g kuh-seng, 10g Cortex Phellodendri, 20g frutus cnidii, the 10g tuber of stemona, 10g Java brucea, the 10g fruit of summer cypress, 15g
Fresh hide, 10g honeysuckle, 10g wild chrysanthemum, 10g Golden Larch Bark, 10g rhizoma acori graminei, 10g rhizoma atractylodis, 10g radix saposhnikoviae, 10g radix scutellariae and 10g Radix Glycyrrhizae
It is extracted, filter, be concentrated, being formulated for raw material.
Now there is the Chinese medicine cleaning solution of the various treatment gynecology colpitis manufactured using Chinese herbal medicine as raw material in the market, but is at present
Only, not yet occur being equipped with Chinese medicine cleaning solution prepared by kuh-seng, radix scutellariae, Cortex Phellodendri with honeysuckle.
Summary of the invention
In view of this, the present invention is directed to propose a kind of Chinese medicine cleaning solution and preparation method thereof for treating gynecology colpitis, with
By all medicines share, improve the symptom of vaginitis rapidly, substitute the use of Western medicine, reduce the recurrent exerbation of this disease, is faced with meeting
Bed demand.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
A kind of Chinese medicine cleaning solution for treating gynecology colpitis, the cleaning solution are made of raw material from the following weight: gold and silver
Spend 5~30 parts, 1~20 part of kuh-seng, 1~10 part of radix scutellariae, 1~10 part of Cortex Phellodendri.
Further, the cleaning solution is made of raw material from the following weight: 15~25 parts of honeysuckle, 5~19 parts of kuh-seng,
2~10 parts of radix scutellariae, 5~9 parts of Cortex Phellodendri.
Further, the weight ratio of the honeysuckle and Cortex Phellodendri is 3:1.
Further, honeysuckle in the cleaning solution, kuh-seng, radix scutellariae, Cortex Phellodendri weight ratio be 3:2:1:1.
A kind of preparation method of the Chinese medicine cleaning solution of above-mentioned treatment gynecology colpitis, comprising:
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 2~15 times of honeysuckle parts by weight amounts, leaching is added
20~60min, 95 DEG C~100 DEG C decoction 1h~3h are steeped, are decocted 1~3 time, combined extract is spare；
(3) extracting solution is cooled to room temperature, is filtered, encapsulating, high-temperature sterilization, it is cooling to get treatment gynecology colpitis
Chinese medicine cleaning solution.
Further, the preparation method of the Chinese medicine cleaning solution of the treatment gynecology colpitis, comprising:
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 5~10 times of honeysuckle parts by weight amounts, leaching is added
30~40min, 100 DEG C of slightly boiled decoction 1h~2h are steeped, are decocted 1~2 time, combined extract is spare；
(3) extracting solution is cooled to room temperature, with 300~400 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds,
The cooling Chinese medicine cleaning solution to get treatment gynecology colpitis.
Further, comprising:
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed honeysuckle and Cortex Phellodendri are put into multi-function extractor, 5 times of honeysuckle parts by weight amounts is added
Water impregnates 40min, 100 DEG C of slightly boiled decoction 1h, is cooled to room temperature up to extracting solution A；
(3) kuh-seng and radix scutellariae are put into another multi-function extractor, the water of 5 times of honeysuckle parts by weight amounts of addition, 100
DEG C slightly boiled decoction 1h is cooled to room temperature up to extracting solution B；
(4) it mixes after filtering the extracting solution A and the extracting solution B respectively, after 50~60 DEG C of decoction 0.5h, is cooled to
Room temperature, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, be cooled to room temperature to get it is described treatment gynecology colpitis Chinese medicine cleaning solution.
Compared with the existing technology, the Chinese medicine cleaning solution and preparation method thereof for the treatment of gynecology colpitis of the present invention has
Following advantage:
(1) it is of the present invention treatment gynecology colpitis Chinese medicine cleaning solution compatibility rationally, all medicines share, can eliminating dampness stop
Itch, be clearing heat and detoxicating, antibacterial anti-inflammatory, rapidly improve vaginitis symptom, have no toxic side effect, it is highly-safe, have it is good it is anti-inflammatory,
Antibacterial, itching-relieving action can substitute the use of Western medicine, can effectively reduce the recurrent exerbation of this disease.
(2) in the Chinese medicine cleaning solution for the treatment of gynecology colpitis of the present invention, the weight ratio of honeysuckle and Cortex Phellodendri exists
Unique relevance, when the weight ratio of honeysuckle and Cortex Phellodendri is 3:1, cleaning solution of the present invention to staphylococcus aureus and
The inhibiting effect of Candida albicans is more preferably, especially fairly obvious to the inhibiting effect of staphylococcus aureus.
(3) in the Chinese medicine cleaning solution for the treatment of gynecology colpitis of the present invention, when honeysuckle, kuh-seng, radix scutellariae, Cortex Phellodendri
When weight ratio is 3:2:1:1, the anti-inflammatory effect of the Chinese medicine cleaning solution is more excellent.
(3) preparation method of the Chinese medicine cleaning solution for the treatment of gynecology colpitis of the present invention uses four taste natural Chinese traditional medicines
It processing, medicine source is abundant, and component compatibility is reasonable, scientific, and process stabilizing is controllable, effective component in prescription can be effectively obtained,
Keep pharmaceutical properties stable, reliable, in addition, the method is simple, convenient, not only increases production efficiency, and effectively reduce
Production cost.
(4) preparation method of the Chinese medicine cleaning solution for the treatment of gynecology colpitis of the present invention is by using honeysuckle and Huang
Cypress prepares extracting solution A, prepares extracting solution B by kuh-seng and radix scutellariae, then extracting solution A and extracting solution B are mixed with cleaning solution, makes
The itching-relieving action for obtaining the Chinese medicine cleaning solution is greatly improved, while can ensure that the Chinese medicine cleaning solution has preferable heat-clearing
Eliminating dampness effect.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained under that premise of not paying creative labor belongs to the model that the present invention protects
It encloses.
Embodiment 1:
One, it is formulated
(1) raw material: honeysuckle 5kg, kuh-seng 20kg, radix scutellariae 2kg, Cortex Phellodendri 5kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 2 times of honeysuckle parts by weight amounts is added, impregnated
20min, 95 DEG C of decoction 1h, decoct 1 time to get extracting solution, it is spare；
(3) extracting solution is cooled to room temperature, with 300 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
Embodiment 2:
One, it is formulated
(1) raw material: honeysuckle 30kg, kuh-seng 5kg, radix scutellariae 1kg, Cortex Phellodendri 10kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 15 times of honeysuckle parts by weight amounts is added, impregnated
60min, 100 DEG C of slightly boiled decoction 3h are decocted 3 times, and combined extract is spare；
(3) extracting solution is cooled to room temperature, with 300 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
Embodiment 3:
One, it is formulated
(1) raw material: honeysuckle 25kg, kuh-seng 1kg, radix scutellariae 10kg, Cortex Phellodendri 1kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 10 times of honeysuckle parts by weight amounts is added, impregnated
30min, 100 DEG C of slightly boiled decoction 2h are decocted 2 times, and combined extract is spare；
(3) extracting solution is cooled to room temperature, with 350 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
Embodiment 4:
One, it is formulated
(1) raw material: honeysuckle 15kg, kuh-seng 19kg, radix scutellariae 5kg, Cortex Phellodendri 9kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 5 times of honeysuckle parts by weight amounts is added, impregnated
40min, 100 DEG C of slightly boiled decoction 1.5h are decocted 1 time, and combined extract is spare；
(3) extracting solution is cooled to room temperature, with 400 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
Embodiment 5:
One, it is formulated
(1) raw material: honeysuckle 15kg, kuh-seng 6kg, radix scutellariae 5kg, Cortex Phellodendri 5kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 8 times of honeysuckle parts by weight amounts is added, impregnated
35min, 100 DEG C of slightly boiled decoction 1h are decocted 1 time, and combined extract is spare；
(3) extracting solution is cooled to room temperature, with 300 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
Embodiment 6:
One, it is formulated
(1) raw material: honeysuckle 18kg, kuh-seng 7kg, radix scutellariae 4kg, Cortex Phellodendri 6kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 10 times of honeysuckle parts by weight amounts is added, impregnated
40min, 100 DEG C of slightly boiled decoction 1h are decocted 1 time, and combined extract is spare；
(3) extracting solution is cooled to room temperature, with 400 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
Embodiment 7:
One, it is formulated
(1) raw material: honeysuckle 18kg, kuh-seng 12kg, radix scutellariae 6kg, Cortex Phellodendri 6kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 10 times of honeysuckle parts by weight amounts is added, impregnated
40min, 100 DEG C of slightly boiled decoction 1h are decocted 1 time, and combined extract is spare；
(3) extracting solution is cooled to room temperature, with 400 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
Embodiment 8:
One, it is formulated
(1) raw material: honeysuckle 18kg, kuh-seng 12kg, radix scutellariae 6kg, Cortex Phellodendri 6kg.
Two, preparation method
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed honeysuckle and Cortex Phellodendri are put into multi-function extractor, 5 times of honeysuckle parts by weight amounts is added
Water impregnates 40min, 100 DEG C of slightly boiled decoction 1h, is cooled to room temperature up to extracting solution A；
(3) kuh-seng and radix scutellariae are put into another multi-function extractor, the water of 5 times of honeysuckle parts by weight amounts of addition, 100
DEG C slightly boiled decoction 1h is cooled to room temperature up to extracting solution B；
(4) it mixes after filtering the extracting solution A and the extracting solution B respectively, after 50-60 DEG C of decoction 0.5h, is cooled to
Room temperature, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, be cooled to room temperature to get it is described treatment gynecology colpitis Chinese medicine cleaning solution.
Experimental example 1: drug urgency toxicity and drug long term toxicity test
It tested using short-term models of passive skin irritability and carries out drug urgency toxicity research, carried out using long term vascular irritant experiment
The research of drug long term toxicity, and then the safety of cleaning solution of the present invention is studied.
One, experimental material:
(1) experimental animal
SD rat, half male and half female, 250~300g of weight (SPF grades), white big ear rabbit, 2.5 ± 0.5kg of weight are (common
Grade), every animal is numbered and is weighed, is tested after quarantine observation 3d.Appearance sign, behavior are carried out to experimental animal
Activity, food ration and weight observation.Feeding environment, 19~26 DEG C of room temperature, temperature difference per day≤4 DEG C, relative humidity 40%~70%, daytime
Night light and shade alt time 12h/12h.Animal for research is purchased from Chinese Academy of Medical Sciences's Experimental Animal Center.
(2) laboratory sample and reagent
Experiment reagent: ovalbumin, Amy victory Science and Technology Ltd.；
Laboratory sample:
D1: honeysuckle cleaning solution: honeysuckle 40kg is put into multi-function extractor, and 8 times of honeysuckle parts by weight amounts are added
Water, impregnate 30min, 100 DEG C of slightly boiled decoction 2h, decoct 2 times, extracting solution is cooled to room temperature by combined extract, filter, fill
Envelope, high-temperature sterilization are cooling to get honeysuckle cleaning solution.
D2: radix scutellariae cleaning solution: radix scutellariae 40kg is put into multi-function extractor, and the water of 8 times of radix scutellariae parts by weight amounts, leaching is added
30min, 100 DEG C of slightly boiled decoction 2h are steeped, are decocted 2 times, extracting solution is cooled to room temperature by combined extract, filtering, encapsulating, high temperature
Sterilizing, it is cooling to get radix scutellariae cleaning solution.
D3: kuh-seng cleaning solution: kuh-seng 40kg is put into multi-function extractor, and the water of 8 times of kuh-seng parts by weight amounts, leaching is added
30min, 100 DEG C of slightly boiled decoction 2h are steeped, are decocted 2 times, extracting solution is cooled to room temperature by combined extract, filtering, encapsulating, high temperature
Sterilizing, it is cooling to get kuh-seng cleaning solution.
D4: Cortex Phellodendri cleaning solution: Cortex Phellodendri 40kg is put into multi-function extractor, and the water of 8 times of Cortex Phellodendri parts by weight amounts, leaching is added
30min, 100 DEG C of slightly boiled decoction 2h are steeped, are decocted 2 times, extracting solution is cooled to room temperature by combined extract, filtering, encapsulating, high temperature
Sterilizing, it is cooling to get Cortex Phellodendri cleaning solution.
D5: radix scutellariae, kuh-seng, Cortex Phellodendri combined type cleaning liquid: radix scutellariae 10kg, kuh-seng 20kg, Cortex Phellodendri 10kg put into multi-functional mention
It takes in tank, the water of 8 times of total weight parts amounts is added, impregnate 30min, 100 DEG C of slightly boiled decoction 2h, decoct 2 times, combined extract, it will
Extracting solution is cooled to room temperature, filtering, encapsulating, high-temperature sterilization, cooling to get radix scutellariae, kuh-seng, Cortex Phellodendri combined type cleaning liquid.
D6: honeysuckle, kuh-seng, Cortex Phellodendri combined type cleaning liquid: honeysuckle 30kg, kuh-seng 20kg, Cortex Phellodendri 10kg put into more function
In energy extractor, the water of 8 times of total weight parts amounts are added, impregnate 30min, 100 DEG C of slightly boiled decoction 2h, decoct 2 times, merging is extracted
Extracting solution is cooled to room temperature by liquid, filtering, encapsulating, high-temperature sterilization, cooling to get honeysuckle, kuh-seng, Cortex Phellodendri combined type cleaning liquid.
D7: honeysuckle, radix scutellariae, Cortex Phellodendri combined type cleaning liquid: honeysuckle 30kg, radix scutellariae 10kg, Cortex Phellodendri 10kg put into more function
In energy extractor, the water of 8 times of total weight parts amounts are added, impregnate 30min, 100 DEG C of slightly boiled decoction 2h, decoct 2 times, merging is extracted
Extracting solution is cooled to room temperature by liquid, filtering, encapsulating, high-temperature sterilization, cooling to get honeysuckle, radix scutellariae, Cortex Phellodendri combined type cleaning liquid.
D8: honeysuckle, radix scutellariae, kuh-seng combined type cleaning liquid: honeysuckle 30kg, radix scutellariae 10kg, kuh-seng 20kg put into more function
In energy extractor, the water of 8 times of total weight parts amounts are added, impregnate 30min, 100 DEG C of slightly boiled decoction 2h, decoct 2 times, merging is extracted
Extracting solution is cooled to room temperature by liquid, filtering, encapsulating, high-temperature sterilization, cooling to get honeysuckle, radix scutellariae, kuh-seng combined type cleaning liquid.
D9: it 1 cleaning solution of the embodiment of the present invention: is prepared using the formula and preparation method of embodiment 1.
D10: it 2 cleaning solution of the embodiment of the present invention: is prepared using the formula and preparation method of embodiment 3.
D11: it 3 cleaning solution of the embodiment of the present invention: is prepared using the formula and preparation method of embodiment 5.
D12: it 4 cleaning solution of the embodiment of the present invention: is prepared using the formula and preparation method of embodiment 6.
D13: it 5 cleaning solution of the embodiment of the present invention: is prepared using the formula and preparation method of embodiment 7.
D14: it 6 cleaning solution of the embodiment of the present invention: is prepared using the formula and preparation method of embodiment 8.
D15 (contrast groups): cleaning solution is prepared in a manner of 201210357058.2 embodiments 1.
D16 (contrast groups): cleaning solution is prepared with 201510985104.7 specific embodiment.
D17: honeysuckle, Cortex Phellodendri combined type cleaning liquid: honeysuckle 30kg, Cortex Phellodendri 10kg are put into multi-function extractor, are added
Enter the water of 8 times of total weight parts amounts, impregnate 30min, 100 DEG C of slightly boiled decoction 2h, decocts 2 times, combined extract, extracting solution is cooling
To room temperature, filtering, encapsulating, high-temperature sterilization, cooling is to get honeysuckle, Cortex Phellodendri combined type cleaning liquid.
Two, experimental method
(1) short-term models of passive skin irritability experiment: the cleaning solution of the sample D1~D16, which is condensed into crude drug concentration, is
0.80g/ml solution, 4 DEG C save, are spare, and when use restores to room temperature.SD rat 54 are taken, is randomly divided into 18 groups: positive control
Group, negative control group, D1~D16 cleaning solution group, every group 3.Relative medicine sensitization is injected respectively to each group SD rat abdominal cavity, sun
Property control group (ovalbumin 3mg/ only), negative control group (0.9% sodium chloride injection), D1~D16 cleaning solution group it is (corresponding clear
Washing lotion 3mg/ is only), the next day 1 time, totally 5 times.It taking a blood sample within the 12nd day after last sensitizing injection, blood 2000r/min is centrifuged 10min, point
From serum, -20 DEG C of preservations are spare in 2 weeks.
Separately SD rat 54 is taken only to be randomly divided into 18 groups: positive controls, negative control group, D1~D16 cleaning solution group, every group
3.Lose hair or feathers 3 × 4cm at each SD rat two sides back2, above-mentioned each group antiserum normal saline dilution respectively takes at 1:2 and 1:8
0.1ml distinguishes intracutaneous injection at left and right sides of respective sets rat back.Each group intravenous injection is identical with priming dose after for 24 hours swashs
Hair antigen adds the total 1ml excitation of 0.5% Evans blue dyestuff of equivalent.Groups of animals abdominal aorta after 20min is excited to take a blood sample, centrifugation
It takes serum to measure SERUM IgE content, puts to death animal after blood sampling at once, measure the blue spot size of skin inner layer, diameter > 5mm
Person is the positive.
(2) cleaning solution of described sample D9, D15 and D16 long term vascular irritant experiment: are condensed into crude drug concentration respectively
For the solution of 0.80g/ml, 0.5g/ml, 0.2g/ml, 4 DEG C save, are spare, and when use restores to room temperature.Take white big ear rabbit 18
Only, 9 groups are randomly divided into, grouping situation is shown in Table 1.It is quiet to each group rabbit ear edge by 0.5ml/kg weight according to shown in table 1
Arteries and veins left and right ear instillation drug, is administered once daily, continuous 14d, before daily administration and after the last administration 48h to animal and
Injection site is visually observed, and observing and recording injection site, whether there is or not redness, hyperemia etc. to stimulate phenomenon.48h is every after the last administration
Group puts to death half animal, with auricular vein injection point to centripetal direction (avoiding injection site) 2cm, continuously take 3 sections of blood vessels together with
Surrounding tissue, every section of 1cm are fixed with 10% formalin, do histopathologic examination, and observation venous vascular tissue has no inflammation
Reaction, or even the conspicuousnesses stimulate the reaction such as denaturation, necrosis.Remaining animal continues to carry out histopathological examination again after observing 14d,
To understand the invertibity of irritative response.
The requirement of 1 long term vascular irritant experiment of table
Three, experimental result
(1) short-term models of passive skin irritability experiment: after excitation, locus coeruleus situation and SERUM IgE content at left and right sides of each group rat
It is shown in Table 2, by statistical calculations, experimental result has statistical significance (P is less than 0.01).
2 drug urgency toxicity test result of table
Can be obtained by upper table 2: compared with negative control group, positive controls SERUM IgE content obviously increases (P <
0.01), each cleaning solution group SERUM IgE content of D1~D14, which has no, substantially change, and D15 and two groups of SERUM IgE contents of D16 slightly rise
It is high.The above result shows that cleaning solution of the present invention does not cause allotransplantation in rats passive cutaneous anaphylaxis, explanation under the experiment condition
The short-term large dosage of Chinese medicine cleaning solution of the present invention is safe and reliable.And with D15 compared with D16 cleaning solution group, illustrate in the present invention
The drug urgency toxicity of medicine cleaning solution is lower, and short-term large dosage use is more safe and reliable.
(2) long term vascular irritant experiment the results are shown in Table shown in 3, and by statistical calculations, experimental result has statistics
Meaning (P is less than 0.01).
3 long term vascular irritant experiment result of table
It can be obtained by upper table 3: during the experiment, the experimental animal of the high, medium and low dosage group of cleaning solution D9 of the present invention
Left and right ear administration local vascular is showed no any stimulate the reaction symptom, and stimulate the reaction scoring is 0 point；Pathological examination result
Display administration terminated and at the end of convalescence, 0.9% sodium chloride injection control sides of experimental animal and high, medium and low dosage group blood
Pipe and surrounding tissue are showed no obvious abnormalities.Illustrate under this experimental condition, injects cleaning of the present invention to rabbit vein
The high, medium and low dosage of liquid D9, it is nonirritant to injection site, illustrate that safety is used for a long time in washing lotion of the present invention.
Left ear show swells D15 high dose group during the experiment, hyperemia stimulates phenomenon, and left ear has inflammation anti-at the end of administration
It answers, and left ear still has inflammatory reaction at the end of convalescence, irritative response has irreversibility.
Find in D16 high dose group and D16 middle dose group: left ear show swells during the experiment, hyperemia stimulates phenomenon,
Left ear has inflammatory reaction at the end of administration, but left ear inflammatory reaction disappears at the end of convalescence, and irritative response has invertibity.
It is available by the above results: under this experimental condition, cleaning solution D9 of the present invention compared with D15, D16,
It is weaker to injection site irritation, it is not easy to cause inflammation, washing lotion of the present invention is used for a long time safer.
Experimental example 2: cleaning solution anti-inflammatory effect research
One, experiment reagent and sample:
(1) experiment reagent:
Histamine: institute of materia medica of China Medical institute；
Dimethylbenzene: Beijing Fine Chemical Works；
Her Wen is blue: the production of Serva company of West Germany, the packing of Solution on Chemical Reagents in Shanghai factory；
0.9% physiological saline: Beijing Sen Yousi Pharmaceuticals Ltd；
JIEERYIN XIYE: Sichuan Enwei Pharmaceutical Co., Ltd..
(2) laboratory sample:
The resulting sample D1~D16 of experimental example 1 is concentrated into the solution that concentration containing crude drug is 0.80g/ml respectively, is then made
Standby wet tissue, it is spare.
D18: spare up to D18 cleaning solution after JIEERYIN XIYE is diluted 10 times.
Two, experimental animal:
Male Kunming strain mice, weight 25-30g are purchased from Chinese Academy of Medical Sciences's Experimental Animal Center.
Three, experimental method:
Take weight 25-30g, male Kunming strain mice 36, be randomly divided into 18 groups: control group, D1~D16 cleaning solution group,
JIEERYIN XIYE group, every group 2.Physiological saline is smeared, in D1~D16 with cotton balls at every mouse right ear exterior feature of control group
The wet tissue prepared at every mouse right ear exterior feature of cleaning solution group with corresponding cleaning solution is smeared, in every mouse right ear of JIEERYIN XIYE group
D18 cleaning solution is smeared at exterior feature.10 minutes primary, continuous 6 times, amounts to 1 hour.Then it is right 100% dimethylbenzene to be applied to each mouse
Two sides before and after ear, respectively at cause it is scorching after 0.5h, l.5h, 2.5h to each group mouse smear again 3 above-mentioned three kinds of medical fluids (control group,
Physiological saline；D1~D16 cleaning solution group, the wet tissue of corresponding cleaning solution preparation；JIEERYIN XIYE group, D18 cleaning solution), Zuo Eryong
Same amount of normal saline concurrent control；Cause it is scorching after 4 hours by animal sacrificed by decapitation, two ears are cut along auricle base line, by two ears with straight
Diameter puncher lays auricle weighing, and the difference of every two auricle weight of mouse is swelling.
Four, experimental result:
Cleaning solution anti-inflammatory effect result of study is shown in Table 4, and by statistical calculations, experimental result has statistical significance
(P is less than 0.01).
4 cleaning solution anti-inflammatory effect result of study of table
| Group | Weight | Left ear weight g | Auris dextra weight g | Interaural difference value g |
| Control group | 41.83±3.02 | 7.66±0.03 | 9.77±0.27 | 2.11±0.92 |
| D1 cleaning solution group | 41.52±2.98 | 7.62±0.02 | 9.46±0.21 | 1.84±0.85 |
| D2 cleaning solution group | 41.76±2.34 | 7.51±0.03 | 9.47±0.18 | 1.96±0.76 |
| D3 cleaning solution group | 41.27±2.15 | 7.23±0.06 | 9.29±0.14 | 2.06±0.56 |
| D4 cleaning solution group | 41.42±2.07 | 7.29±0.07 | 9.30±0.25 | 2.01±0.27 |
| D5 cleaning solution group | 41.63±2.19 | 7.82±0.02 | 9.56±0.18 | 1.74±0.31 |
| D6 cleaning solution group | 41.94±2.87 | 7.90±0.08 | 9.86±0.17 | 1.96±0.29 |
| D7 cleaning solution group | 41.25±3.07 | 7.15±0.04 | 8.83±0.27 | 1.68±0.46 |
| D8 cleaning solution group | 41.57±3.22 | 7.24±0.01 | 9.11±0.14 | 1.87±0.51 |
| D9 cleaning solution group | 41.61±3.41 | 7.36±0.05 | 8.23±0.12 | 0.87±0.34 |
| D10 cleaning solution group | 41.18±2.08 | 7.31±0.04 | 8.07±0.25 | 0.76±0.32 |
| D11 cleaning solution group | 41.72±2.54 | 7.84±0.06 | 8.25±0.21 | 0.41±0.27 |
| D12 cleaning solution group | 41.69±2.61 | 7.64±0.04 | 8.07±0.16 | 0.43±0.35 |
| D13 cleaning solution group | 41.17±2.77 | 7.61±0.03 | 7.82±0.18 | 0.21±0.26 |
| D14 cleaning solution group | 41.25±3.79 | 7.28±0.07 | 7.47±0.21 | 0.19±0.22 |
| D15 cleaning solution group | 41.23±3.24 | 7.26±0.02 | 8.22±0.17 | 0.96±0.32 |
| D16 cleaning solution group | 41.84±3.57 | 7.45±0.03 | 8.40±0.19 | 0.95±0.29 |
| JIEERYIN XIYE group | 41.16±3.54 | 7.59±0.02 | 8.48±0.13 | 0.89±0.35 |
Can be obtained by table 4: 18 groups of mutual mouse weight ratios are P > 0.05, and there was no significant difference, and 18 groups left each other
Ear heavy phase is than there are no significant difference.
Compared with the control group, interaural difference value (swelling) is without significant difference for D1~D4 cleaning solution group；D5~D8 cleaning solution group
Interaural difference value (swelling) be slightly below control group；The interaural difference value (swelling) of D9~D14 cleaning solution group is significantly lower than control
Group；And the interaural difference value (swelling) of D9~D14 cleaning solution group is lower than D15 cleaning solution group, D16 cleaning solution group and JIEERYIN XIYE
Group illustrates D9~D14 cleaning solution group anti-inflammatory effect of the present invention better than D15 cleaning solution group, D16 cleaning solution group and clean that yin
Washing lotion group.Further, the interaural difference value (swelling) of D11~D14 cleaning solution group of the present invention is lower compared with other groups, and described
The anti-inflammatory effect for inventing D11~D14 cleaning solution group is more excellent.Further, applicant is found surprisingly that, such as D13 of the present invention and D14
Shown in the anti-inflammatory effect result of study of cleaning solution group, when the weight ratio of honeysuckle, kuh-seng, radix scutellariae, Cortex Phellodendri is 3:2:1:1, this
The invention cleaning solution anti-inflammatory effect is more excellent.
Experimental example 3: cleaning solution in vitro activity
One, laboratory sample and reagent
(1) experiment reagent:
Staphylococcus aureus reference culture ATCC25923, escherichia coli reference culture ATCC25922, white are read
The pearl bacterium bacterial strain of resistance to Fluconazole (MIC > 16mg/l).
(2) laboratory sample:
The resulting sample D5~D16 of experimental example 1 is concentrated into the solution that concentration containing crude drug is 0.80g/ml respectively, is then made
Standby wet tissue, it is spare.
Two, experimental method
Using medicine base method, the cleaning solution of D5~D16 is condensed into medicinal extract respectively, with a small amount of 80% ethanol solution of volume fraction
Hydrotropy, then it is made into distilled water the mother liquid medicine of 100g/l, after filtration sterilization, mother liquor is proportionally added into the MH agar of thawing
(Candida albicans sabouraud's agar) mixes bed board, and obtaining every 1ml content of dispersion is respectively 10,5,4,3,2,1,0.5,0.25,
The drug containing agar plate of 0.125mg.With micro sample adding appliance dibbling bacterium solution (bacteria containing amount about 107CFU/ml) 1 μ L one by one, the bacterium of formation
Liquid loop diameter is 5mm or so, and bacterium solution does 37 DEG C of incubation 18h of postposition, and the lowest concentration of drug that bacterium colony growth is totally constrained is should
MIC value of the medicine to detection bacterium.Each test drug sample does 3 experiments in parallel, compares group with the MH agar plate of not drug containing.In vitro
Combination experimental result, is shown in Table 5, and by statistical calculations, experimental result has statistical significance (P is less than 0.01).
The external Combination experimental result of table 5
Can be obtained by upper table 5: cleaning solution of the present invention is to staphylococcus aureus, escherichia coli and Candida albicans
There is obvious inhibiting effect.Comparing D5~D8 cleaning solution group of the present invention and D9~D14 cleaning solution group can obtain: of the present invention
D9~D14 cleaning solution group is more obvious to the inhibiting effect of staphylococcus aureus, escherichia coli and Candida albicans.It is right
Can obtain than D9~D14 cleaning solution group and D15~D16 cleaning solution group: each cleaning solution group is to staphylococcus aureus, escherichia coli
Bacterium and Candida albicans have obvious inhibiting effect, and D11~D14 cleaning solution group is to staphylococcus aureus and Candida albicans
Inhibiting effect more preferably, it is especially fairly obvious to the inhibiting effect of staphylococcus aureus.Applicant sends out in the course of the research
Existing: there are unique relevances in this side for the weight ratio of honeysuckle and Cortex Phellodendri, when the weight ratio of honeysuckle and Cortex Phellodendri is 3:1
When, as shown in the bacteriostatic experiment result of D11~D14 cleaning solution group, cleaning solution of the present invention is to staphylococcus aureus and white
The inhibiting effect of color candida albicans is more preferably, especially fairly obvious to the inhibiting effect of staphylococcus aureus.
Experimental example 4: cleaning solution itching-relieving action research
One, laboratory sample and reagent
(1) experiment reagent:
0.9% physiological saline: Beijing Sen Yousi Pharmaceuticals Ltd；
Histamine phosphate: Tokyo Chemical Industry Co., Ltd；
JIEERYIN XIYE: Sichuan Enwei Pharmaceutical Co., Ltd..
(2) laboratory sample:
Experimental example 1 resulting sample D6, D9~D17 are concentrated into the solution that concentration containing crude drug is 0.80g/ml respectively, so
After prepare wet tissue, it is spare.
D18: up to D18 cleaning solution after JIEERYIN XIYE is diluted 10 times.
(3) experimental animal: cavy, half male and half female, 350 ± 50g of weight (regular grade), animal for research are purchased from middle traditional Chinese medical science
Subject institute Experimental Animal Center.
Two, experimental method
Divide 12 groups at random for 48 cavys, comprising: control group, D6 cleaning solution group, D9~D18 cleaning solution group, every group 4,
It is clean after right metapedes with one's hands clasped or tied behind one's back hair position soak (adhesive tape is fixed) physiological saline (control group) of each group cavy, 10 times of dilution
Cleaning solution wet tissue (D6 cleaning solution group, D9~D17 cleaning solution group) made from the negative washing lotion (D18 cleaning solution group) of that, counter sample, 10
Minute liquid feeding is primary, continuous 6 times, amounts to 1 hour；Each position drop 0.01% 50 μ L/ pawl of histamine phosphate, later every
3min drips the histamine phosphate that increasing concen-trations are 0.02%, 0.03%, 0.04% equivalent at same position again, until cavy licks
Until foot, using the total amount of histamine phosphate for giving each animal to be dripped in this time as itch-threshold, and by each animal in every group
Itch-threshold and skin thickness are analyzed.
Three, experimental result
Cleaning solution itching-relieving action result of study of the present invention is shown in Table 6, and by statistical calculations, experimental result has statistics
It learns meaning (P is less than 0.01).
6 itching-relieving action result of study of table
Can be obtained by table 6: compared with the control group, itch-threshold has notable difference to D6, D9~D18 cleaning solution group, illustrate D6, D9~
D18 cleaning solution group all has apparent itching-relieving action.Comparing D9~D14 cleaning solution group of the present invention can obtain, and D11~D14 is clear
The itching-relieving action of washing lotion group is substantially better than D9~D10 cleaning solution group.Compare D11~D14 cleaning solution group and D15, D16, D18 cleaning
Liquid group can obtain, and the itching-relieving action of D11~D14 cleaning solution group is better than D15, D16, D18 cleaning solution group.Comparison D11~D14 is respectively cleaned
Liquid group can obtain, and the itching-relieving action of D14 cleaning solution group is greatly improved, applicant carried out further research, by D6,
The itching-relieving action experimental result of D17, D9, D14 are found: although it is known that playing the ingredient of itching-relieving action in the cleaning solution
Predominantly kuh-seng, if but decoct honeysuckle, Cortex Phellodendri and kuh-seng together, but it is unfavorable for the performance of kuhseng anti-itch effect, antipruritic
It acts in experimental result, the itching-relieving action of D6 is differed with the itching-relieving action of D17 and is not obvious, and is worked as honeysuckle, kuh-seng, Huang
When a kind of reed mentioned in ancient books, Cortex Phellodendri decoct together, the itching-relieving action of kuh-seng can obtain a degree of performance.Further, when by honeysuckle and
Cortex Phellodendri individually decocts, and when mixing later with the decoction liquor of kuh-seng and radix scutellariae, can further effectively facilitate kuh-seng in the cleaning solution
The performance of itching-relieving action improves the antipruritic effect of cleaning solution.In addition, applicants have found that, when honeysuckle and Cortex Phellodendri is independent
It decocts, when mixing later with the decoction liquor of kuh-seng and radix scutellariae, the cleaning solution not only has preferable itching-relieving action, and can be same
When have good heat-clearing and damp-drying drug effect.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of Chinese medicine cleaning solution for treating gynecology colpitis, which is characterized in that the cleaning solution by following parts by weight raw material
It is made: 5~30 parts of honeysuckle, 1~20 part of kuh-seng, 1~10 part of radix scutellariae, 1~10 part of Cortex Phellodendri.
2. cleaning solution according to claim 1, which is characterized in that the cleaning solution is made of raw material from the following weight:
15~25 parts of honeysuckle, 5~19 parts of kuh-seng, 2~10 parts of radix scutellariae, 5~9 parts of Cortex Phellodendri.
3. cleaning solution according to claim 2, which is characterized in that the weight ratio of the honeysuckle and Cortex Phellodendri is 3:1.
4. cleaning solution according to claim 1, which is characterized in that honeysuckle, kuh-seng, radix scutellariae, Cortex Phellodendri in the cleaning solution
Weight ratio be 3:2:1:1.
5. the preparation method of the Chinese medicine cleaning solution for the treatment of gynecology colpitis according to any one of claims 1-4, feature exist
In, comprising:
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 2~15 times of honeysuckle parts by weight amounts is added, impregnate 20
~60min, 95 DEG C~100 DEG C decoction 1h~3h are decocted 1~3 time, and combined extract is spare；
(3) extracting solution is cooled to room temperature, is filtered, encapsulating, high-temperature sterilization, it is cooling to get in treatment gynecology colpitis
Medicine cleaning solution.
6. the preparation method of the Chinese medicine cleaning solution for the treatment of gynecology colpitis as claimed in claim 5 characterized by comprising
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed medicinal material is put into multi-function extractor, the water of 5~10 times of honeysuckle parts by weight amounts is added, impregnate 30
~40min, 100 DEG C of slightly boiled decoction 1h~2h are decocted 1~2 time, and combined extract is spare；
(3) extracting solution is cooled to room temperature, with 300~400 mesh screens, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, it is cooling,
Up to the Chinese medicine cleaning solution for the treatment of gynecology colpitis.
7. the preparation method of the Chinese medicine cleaning solution for the treatment of gynecology colpitis as claimed in claim 5 characterized by comprising
(1) each medicinal material is weighed according to recipe requirements；
(2) weighed honeysuckle and Cortex Phellodendri are put into multi-function extractor, the water of 5 times of honeysuckle parts by weight amounts, leaching is added
40min, 100 DEG C of slightly boiled decoction 1h are steeped, are cooled to room temperature up to extracting solution A；
(3) kuh-seng and radix scutellariae are put into another multi-function extractor, the water of 5 times of honeysuckle parts by weight amounts is added, 100 DEG C micro-
Boiling decocts 1h, is cooled to room temperature up to extracting solution B；
(4) it is mixed after filtering the extracting solution A and the extracting solution B respectively, after 50~60 DEG C of decoction 0.5h, is cooled to room
Temperature, encapsulating, 130 DEG C high-temperature sterilization 10 seconds, be cooled to room temperature to get it is described treatment gynecology colpitis Chinese medicine cleaning solution.
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| CN111821397A (en) * | 2020-06-08 | 2020-10-27 | 晒欧国际生物科技(广州)有限公司 | Environment-friendly rapid bactericide |
| CN112316012A (en) * | 2020-11-19 | 2021-02-05 | 陕西康乐实业有限公司 | Moisturizing antibacterial gel and preparation method thereof |
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