CN109147983A - One kind is based on just setting microscopical single beam laser light forceps device - Google Patents
One kind is based on just setting microscopical single beam laser light forceps device Download PDFInfo
- Publication number
- CN109147983A CN109147983A CN201811089102.XA CN201811089102A CN109147983A CN 109147983 A CN109147983 A CN 109147983A CN 201811089102 A CN201811089102 A CN 201811089102A CN 109147983 A CN109147983 A CN 109147983A
- Authority
- CN
- China
- Prior art keywords
- laser
- light
- setting
- dichroscope
- microcobjective
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21K—TECHNIQUES FOR HANDLING PARTICLES OR IONISING RADIATION NOT OTHERWISE PROVIDED FOR; IRRADIATION DEVICES; GAMMA RAY OR X-RAY MICROSCOPES
- G21K1/00—Arrangements for handling particles or ionising radiation, e.g. focusing or moderating
- G21K1/006—Manipulation of neutral particles by using radiation pressure, e.g. optical levitation
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- High Energy & Nuclear Physics (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
One kind is based on just setting microscopical single beam laser light forceps device, including laser, optical isolator, reflecting mirror, dichroscope, light beam hoisting frame, microcobjective, motorized precision translation stage, Charge Coupled Device.The present invention is based on just setting microscopical light forceps device, to realize demand that laser optical tweezer places the prone position that the single red blood cell of the living body cerebrovascular captures.The present invention will effectively facilitate vivo applications of the optical tweezer technology in brain science field.
Description
Technical field
The present invention relates to the laser optical tweezer that a kind of single beam laser of continuous wave laser transmitting is formed in the case where just setting microscope, tools
Body is that one kind is based on just setting microscopical single beam laser light forceps device.
Background technique
Optical tweezer is the new tool for manipulating micro-nano magnitude particle and measuring skin ox magnitude power.Optical tweezer, i.e. optical tweezers, have
The ability of optical manipulation and weak interaction measurement.Optical tweezer captures small grain by light and object interaction, in three-dimensional space
Son has the characteristics that no Mechanical Contact, remote-controlled operation, does not interfere ambient enviroment locating for particle.In liquid environment, optical tweezer
It can suspend, fix, arranging, manipulating, constructing, sorting and rotating particle.In addition, optical tweezer applies active force to object by light,
Active force is less than 200pN.Therefore, optical tweezer is also the probe of small power, can be used for the weak interaction measurement of microscopic fields.
Laser optical tweezer is in vitro and vital red cell operation research.Laser optical tweezer is passed through using single beam laser has high number
The microcobjective for being worth aperture carries out strong-focusing and generates three-dimensional ligh trap.Optical tweezer can be big to single biology close under physiological status
Molecule, organelle or cell are identified, the vital movement of biological cell is tracked；It is especially red to single red blood cell or lesion thin
Born of the same parents identify or the emergency reaction of the single red blood cell of long-time real-time monitoring.In mobiles, existing optical tweezer technology is
It is applied in living body ear capillary in the research of red blood cell.
The limitation that laser optical tweezer captures living body cerebrovascular red blood cell.Laser optical tweezer currently used for viviperception is main
It is designed using inverted microscope mode, since this mode is conducive to the observation of biological sample, and is set with other auxiliary
Standby united application.However, laboratory sample in viviperception, is generally placed on by the optical optical tweezers system under the mode with dorsal position
The top of microcobjective, ear's capillary that this mode causes the system to be confined to biopsy sample are studied.And working as forebrain
In the cerebrovascular research of scientific domain, the optical optical tweezers system based on inverted microscope mode is difficult to realize correlation function.Compared to
Microscopy mode is set, the light forceps device for just setting microscopy mode may be implemented to be the living body cerebrovascular, spinovascular research
Living body biological sample can be placed under microcobjective by this mode with prone position, this is that inverted microscope mode cannot achieve
's.
The limitation that light forceps device is applied in brain science field proposes new challenge to the development of optical tweezer technology.
Summary of the invention
The object of the present invention is to provide one kind based on microscopical single beam laser light forceps device is just set, to solve above-mentioned ask
At least one aspect of topic.
According to an aspect of the present invention, it provides one kind to be based on just setting microscopical single beam laser light forceps device, including swashs
Light device, optical isolator, reflecting mirror, dichroscope, light beam hoisting frame, microcobjective, motorized precision translation stage, Charge Coupled Device；
The laser is for emitting single beam laser；
The optical isolator protects laser for reflected laser to be isolated；Face the emission port of laser
Setting；
The reflecting mirror for changing laser the direction of propagation；
The light beam hoisting frame is oppositely arranged with optical isolator, for improving the level height of light beam；Reflecting mirror is placed in
On light beam hoisting frame；
The camera lens and dichroscope of the microcobjective are oppositely arranged at 45 degree, form optical tweezer for focused laser beam；
The dichroscope is penetrated according to wavelength or reflected light, and dichroscope will transmit through light and be introduced into the micro- object just set
In mirror, is focused in the solution of sample cell and form optical tweezer, and capture target sample；
The motorized precision translation stage is placed in below the camera lens of microcobjective, the movement for sample；
It further include illumination path, illumination path includes condenser, halogen lamp, and the scattering light that halogen lamp issues is poly- through condenser
Light provides illumination；
The Charge Coupled Device is used to detect the capture effect of optical tweezer, records catching position；Halogen lamp is saturating through condenser
It penetrates light and reflexes to Charge Coupled Device through dichroscope.Charge Coupled Device is connected with computer, in real-time monitoring sample cell
Sample.
The beneficial effects of the present invention are: the present invention is based on microscopical light forceps device is just set, to realize laser optical tweezer to work
The demand that the prone position of the single red blood cell capture of the body cerebrovascular is placed.The present invention will effectively facilitate optical tweezer technology in brain science field
In vivo applications.
Detailed description of the invention
Fig. 1 is a kind of based on the implementation diagram for just setting microscopical optical optical tweezers system.
1- semiconductor laser, 2- optical isolator, 3- reflecting mirror, 4- reflecting mirror, 5- reflecting mirror, 6- dichroscope, 7-
Microcobjective, 8- moveable stage, 9- condenser, 10- halogen light source, 11- Charge Coupled Device, 12- computer.
Specific embodiment
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, with reference to the accompanying drawing, to this
Invention is further detailed.
Embodiment 1
The green laser that solid state laser exports that a branch of wavelength is 532nm first is introduced into after optical isolator shaping
It in the optical path hoisting frame being made of reflecting mirror, and is introduced into the microcobjective just set by dichroscope, in the molten of sample cell
It is focused in liquid and forms optical tweezer.The parameter of microcobjective: amplification factor 60, numerical aperture 1.0, water immersion objective.At this point, laser function
Rate is 15mW or less.The scattering light that halogen lamp issues provides illumination through condenser optically focused.Pass through the electronics connecting with microcobjective
Coupled apparatus and computer are used to observe the dynamic process in sample cell.
Above-mentioned laser is 532nm solid green laser, MGL-H-532nm type.Microcobjective is Olympus brand,
LUMPLFLN60XW(60×,N.A.1.0).Optical isolator is Daheng's photoelectricity brand；Reflecting mirror is GCC-101102 type broadband
Deielectric-coating high reflection mirror (450-700nm, Φ 25.4)；Dichroscope is Φ 12.7mm, transmitted infrared light, vitreous silica system；Electricity
Sub- coupled apparatus is MER-310-120UC type industrial camera CCD, HxV (656 × 492)；It is provided by Daheng's photoelectricity.Light beam mentions
Rising frame is the level height device that optics commonly improves light beam；Motorized precision translation stage is the common linear motor translation stage of optics.
Embodiment 2
Selecting diameter is 1 μm to 2 μm of polystyrene sphere.And bead is suspended in sample cell with distilled water.It is micro-
The hydroscope that object lens select numerical aperture to be higher than 1.0.Solid state laser exports the green laser that a branch of wavelength is 532nm, through optics
After isolator shaping, be introduced into the optical path hoisting frame being made of reflecting mirror, and be introduced by dichroscope just setting it is micro-
In object lens, is focused in the solution of sample cell and form optical tweezer, and capture polystyrene sphere.Illumination path is introduced as through condenser
The light field imaging of bead provides illumination, and transmitted light reflexes to Charge Coupled Device through dichroscope, remembered by Charge Coupled Device
Record small ball position.Charge Coupled Device is connected with computer, the polystyrene sphere in real-time monitoring sample cell.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (4)
1. one kind is based on just setting microscopical single beam laser light forceps device, it is characterised in that: including laser, be optically isolated
Device, reflecting mirror, dichroscope, light beam hoisting frame, microcobjective, motorized precision translation stage, Charge Coupled Device；
The laser is for emitting single beam laser；
The optical isolator protects laser for reflected laser to be isolated；Face the emission port setting of laser；
The reflecting mirror for changing laser the direction of propagation；
The light beam hoisting frame is oppositely arranged with optical isolator, for improving the level height of light beam；Reflecting mirror is placed in light beam
On hoisting frame；
The camera lens and dichroscope of the microcobjective are oppositely arranged at 45 degree, form optical tweezer for focused laser beam；
The dichroscope is penetrated according to wavelength or reflected light, and dichroscope will transmit through light and be introduced into the microcobjective just set
In, it is focused in the solution of sample cell and forms optical tweezer, and capture target sample；
The motorized precision translation stage is placed in below the camera lens of microcobjective, the movement for sample；
The Charge Coupled Device is oppositely arranged with dichroscope, for detecting the capture effect of optical tweezer, records catching position；Electricity
Sub- coupled apparatus is connected with computer, the sample in real-time monitoring sample cell.
2. one kind according to claim 1 is based on just setting microscopical single beam laser light forceps device, it is characterised in that: also
Including illumination path, illumination path includes condenser, halogen lamp, and the scattering light that halogen lamp issues provides photograph through condenser optically focused
It is bright.
3. one kind according to claim 2 is based on just setting microscopical single beam laser light forceps device, it is characterised in that: halogen
Plain lamp reflexes to Charge Coupled Device through dichroscope through condenser transmitted light.
4. one kind according to claim 1 is based on just setting microscopical single beam laser light forceps device, it is characterised in that: light
Three reflecting mirrors are arranged in beam hoisting frame, change the direction of propagation of laser.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811089102.XA CN109147983A (en) | 2018-09-18 | 2018-09-18 | One kind is based on just setting microscopical single beam laser light forceps device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811089102.XA CN109147983A (en) | 2018-09-18 | 2018-09-18 | One kind is based on just setting microscopical single beam laser light forceps device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109147983A true CN109147983A (en) | 2019-01-04 |
Family
ID=64814683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811089102.XA Pending CN109147983A (en) | 2018-09-18 | 2018-09-18 | One kind is based on just setting microscopical single beam laser light forceps device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109147983A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113053556A (en) * | 2021-03-10 | 2021-06-29 | 暨南大学 | Biological micromotor array with reconfigurability and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2610356Y (en) * | 2003-04-07 | 2004-04-07 | 中国船舶重工集团公司第七一一研究所 | Multi-freedom light-beam lift frame |
| US20050008045A1 (en) * | 2002-08-29 | 2005-01-13 | Jinchun Xie | Laser with reflective etalon tuning element |
| US20080310009A1 (en) * | 2007-06-18 | 2008-12-18 | National Yang-Ming University | Device for trapping or stretching microscopic substance and method thereof |
| CN104749105A (en) * | 2015-04-21 | 2015-07-01 | 武汉大学 | Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence |
| US20170205612A1 (en) * | 2014-07-09 | 2017-07-20 | Ntp Nano Tech Projects S.R.L. | Laser optical coupling for nanoparticles detection |
| CN206893310U (en) * | 2017-03-30 | 2018-01-16 | 鲁东大学 | A kind of controllable Optical Tweezers Array device of three-dimensional position |
| CN208834754U (en) * | 2018-09-18 | 2019-05-07 | 湖北第二师范学院 | One kind is based on just setting microscopical single beam laser light forceps device |
-
2018
- 2018-09-18 CN CN201811089102.XA patent/CN109147983A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050008045A1 (en) * | 2002-08-29 | 2005-01-13 | Jinchun Xie | Laser with reflective etalon tuning element |
| CN2610356Y (en) * | 2003-04-07 | 2004-04-07 | 中国船舶重工集团公司第七一一研究所 | Multi-freedom light-beam lift frame |
| US20080310009A1 (en) * | 2007-06-18 | 2008-12-18 | National Yang-Ming University | Device for trapping or stretching microscopic substance and method thereof |
| US20170205612A1 (en) * | 2014-07-09 | 2017-07-20 | Ntp Nano Tech Projects S.R.L. | Laser optical coupling for nanoparticles detection |
| CN104749105A (en) * | 2015-04-21 | 2015-07-01 | 武汉大学 | Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence |
| CN206893310U (en) * | 2017-03-30 | 2018-01-16 | 鲁东大学 | A kind of controllable Optical Tweezers Array device of three-dimensional position |
| CN208834754U (en) * | 2018-09-18 | 2019-05-07 | 湖北第二师范学院 | One kind is based on just setting microscopical single beam laser light forceps device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113053556A (en) * | 2021-03-10 | 2021-06-29 | 暨南大学 | Biological micromotor array with reconfigurability and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208834754U (en) | One kind is based on just setting microscopical single beam laser light forceps device | |
| JP6513802B2 (en) | Laser light coupling for nanoparticle detection | |
| JP2007500529A (en) | Method and apparatus with variable numerical aperture for determining the properties of fluid flowing through a biological tube structure | |
| US20070008528A1 (en) | Device and method for simultaneous optical trapping, stretching, and real-time detection and measurement for morphological deformation of micro-particles | |
| US20240011888A1 (en) | Analysis of tissue samples using quantitative phase-contrast microscopy | |
| CN108956562B (en) | Light section fluorescence microscopic imaging method and device based on repositioning | |
| CN113514945B (en) | Method and apparatus for microscopy | |
| CN110993140A (en) | Single-core optical fiber-multi-core optical fiber combined optical motor system | |
| US7982194B2 (en) | Single nanoparticle tracking spectroscopic microscope | |
| CN109147983A (en) | One kind is based on just setting microscopical single beam laser light forceps device | |
| CN108873283B (en) | Multi-mode full-spectrum dark-field microscope constructed based on super-continuous laser and application thereof | |
| Tian et al. | Blood cell analysis: from traditional methods to super-resolution microscopy | |
| WO2008021202A2 (en) | Capillary-based cell and tissue acquisition system (ctas) | |
| CN113466111A (en) | Single cell analysis system and method and application | |
| Korobtsov et al. | Optical tweezers technique for the study of red blood cells deformation ability | |
| CN110993141A (en) | Multi-core optical fiber suspension type optical motor system | |
| WO2016166847A1 (en) | Observation device | |
| CN113608287B (en) | Micro-lens and application thereof | |
| US20220326502A1 (en) | Apparatuses, systems and methods for solid immersion meniscus lenses | |
| CN109182087A (en) | The method captured in miniflow with manipulation single bacteria based on cataclysm conical fiber | |
| CN108387720A (en) | A kind of device and method for measuring human body thrombin convergent force | |
| WO2017170805A1 (en) | Multi-surface image acquisition system, observation device, observation method, screening method, and stereoscopic reconstruction method of subject | |
| CN113514442A (en) | Dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber optical control | |
| CN113533175A (en) | Living body single cell accurate active optical control method and device based on four-core optical fiber | |
| CN108893250A (en) | A method of zooblast is exchanged using light forceps device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |