CN109142488A - A kind of electrochemistry test paper and its application method with impedance identification - Google Patents
A kind of electrochemistry test paper and its application method with impedance identification Download PDFInfo
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- CN109142488A CN109142488A CN201811122664.XA CN201811122664A CN109142488A CN 109142488 A CN109142488 A CN 109142488A CN 201811122664 A CN201811122664 A CN 201811122664A CN 109142488 A CN109142488 A CN 109142488A
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- 238000012360 testing method Methods 0.000 title claims abstract description 63
- 230000005518 electrochemistry Effects 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 9
- 239000011248 coating agent Substances 0.000 claims abstract description 15
- 238000000576 coating method Methods 0.000 claims abstract description 15
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical group [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229910052737 gold Inorganic materials 0.000 claims abstract description 12
- 239000010931 gold Substances 0.000 claims abstract description 12
- 239000000758 substrate Substances 0.000 claims abstract description 11
- -1 polyethylene terephthalate Polymers 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims abstract description 9
- 238000003487 electrochemical reaction Methods 0.000 claims abstract description 7
- 125000006850 spacer group Chemical group 0.000 claims abstract description 7
- 239000004743 Polypropylene Substances 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 6
- 229920001155 polypropylene Polymers 0.000 claims abstract description 6
- 239000004800 polyvinyl chloride Substances 0.000 claims abstract description 5
- RRKGBEPNZRCDAP-UHFFFAOYSA-N [C].[Ag] Chemical compound [C].[Ag] RRKGBEPNZRCDAP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229920000915 polyvinyl chloride Polymers 0.000 claims abstract description 4
- 239000008280 blood Substances 0.000 claims description 14
- 210000004369 blood Anatomy 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims description 5
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 claims 1
- 238000010248 power generation Methods 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 9
- 238000005259 measurement Methods 0.000 abstract description 8
- 238000005530 etching Methods 0.000 abstract description 5
- 239000005020 polyethylene terephthalate Substances 0.000 abstract description 5
- 229920000139 polyethylene terephthalate Polymers 0.000 abstract description 5
- 230000000694 effects Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000002848 electrochemical method Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000007705 chemical test Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000013095 identification testing Methods 0.000 description 1
- 238000000608 laser ablation Methods 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Electrochemistry (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to electrochemical technology fields, disclose a kind of electrochemistry test paper and its application method with impedance identification, are successively bonded substrate layer, an electrochemical reaction cavity, enzyme layer, coating and the spacer layer for protecting coating upper end from the bottom to top.The electrode layer is gold electrode or carbon silver electrode.The substrate layer uses polyethylene terephthalate, polyvinyl chloride, any one material in polypropylene.The electrode layer is made of the gold electrode of electrode, trigger electrode, connection electrode and impedance identified areas electrochemical working electrode, electrochemistry；The present invention realizes the electrochemistry test paper technology with impedance mark for the first time.So that the test paper of different batches, by being tested instrument automatic identification to the measurement of etching impedance, to achieve the purpose that automatic calibration parameter.
Description
Technical field
The invention belongs to electrochemical technology field more particularly to a kind of electrochemistry test paper with impedance identification and its users
Method.
Background technique
Currently, the prior art commonly used in the trade is such thatCharacterizing human body, whether Jian Kang index has very much, for example blood is normal
Rule, blood lipid, blood pressure, blood glucose, heart rate, acid or alkali environment etc. in human body, and these indexs are needed to the hospital of profession, use profession
Measuring instrument can just measure, for sub-health population or patient with corresponding illness, above-mentioned measurement is very
Therefore there is the portable tester of some households, such as blood-sugar detecting instrument on the market in inconvenience, blood lipid tester, uric acid are surveyed
Try instrument, cholesterol test instrument etc. and corresponding test paper.In the manufacturing of test paper, in the printing of carbon electrode etc., due to manufacture
The error of technique causes the width of every batch of sub-electrode, and thickness etc. cannot be consistent completely, and there are also printing technologies of enzyme etc. to cause
For different batches finished product, the impedance of electrochemistry test paper can be different, finally when electrochemical measurement blood parameters, can generate
Error, CV (coefficient of variation) value will increase.
In conclusion problem of the existing technology is:The width of every batch of sub-electrode, thickness in the manufacturing of test paper
Etc. cannot be consistent completely, the impedance of different batches finished product electrochemistry test paper can be different, in electrochemical measurement blood parameters
When, error can be generated, CV (coefficient of variation) value will increase.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of electrochemistry test paper with impedance identification and its uses
Method.
The invention is realized in this way a kind of electrochemistry test paper with impedance identification, the electrochemistry with impedance identification
Test paper is successively bonded substrate layer, an electrochemical reaction cavity, enzyme layer, coating and for protecting coating upper end from the bottom to top
Spacer layer.
Further, the electrode layer is gold electrode or carbon silver electrode.
Further, the substrate layer uses polyethylene terephthalate, polyvinyl chloride, any one material in polypropylene
Material.
Further, the electrode layer is by electrochemical working electrode, electrochemistry to electrode, trigger electrode, connection electrode and resistance
The gold electrode of anti-identified areas forms；
According to end of sucking blood top distance by remote sequence being closely successively electrochemical working electrode, electrochemistry to electricity
Pole, trigger electrode and connect electrode.
Further, the connection electrode is arranged at the insertion end of electrode layer, connect electrode and electrochemistry to electrode it
Between be arranged a gold electrode region.
Further, there is stomata in the coating upper end.
In conclusion advantages of the present invention and good effect are as follows:The present invention realizes the electricity with impedance mark for the first time
Chemical test paper technology.So that the test paper of different batches, by being tested instrument automatic identification to the measurement of etching impedance, to reach
The purpose of automatic calibration parameter.
Detailed description of the invention
Fig. 1 is the electrochemistry test paper structural schematic diagram provided in an embodiment of the present invention with impedance identification；
In figure: 1, substrate layer；2, electrochemical working electrode；3, trigger electrode；4, impedance identified areas；5, electrode is connected；
6, electrochemistry is to electrode；7, siphon port；8, electrochemical reaction cavity；9, enzyme layer；10, stomata；11, coating；12, spacer layer.
Fig. 2 is the curve of different batches test paper provided in an embodiment of the present invention corresponding measured object concentration and surveyed electric current
Figure.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
The present invention provides a kind of electrochemistry test paper batch identification technology with impedance mark, exempts from bar code test to realize,
Reduce CV (coefficient of variation) value between different batches test paper.The novel test paper technology includes the ginseng after testing impedance and impedance matching
Number allotment.
As shown in Figure 1, the electrochemistry test paper provided in an embodiment of the present invention with impedance identification includes: substrate layer 1, electrochemistry
Working electrode 2, impedance identified areas 4, connects electrode 5, electrochemistry to electrode 6, siphon port 7, electrochemical reaction at trigger electrode 3
Cavity 8, enzyme layer 9, stomata 10, coating 11, spacer layer 12.
Electrochemistry test paper provided in an embodiment of the present invention with impedance identification is from the bottom to top successively by 1, electricity of substrate layer
Chemically react cavity 8, enzyme layer 9, coating 11 and for protecting the spacer layer 12 of 11 upper end of coating to be sticked to form.One electrification
Reaction cavity 8 is learned, cavity includes the electrode layer of electrochemical working electrode 2.
Electrode layer is gold electrode or carbon silver electrode
Substrate layer 1 uses polyethylene terephthalate, polyvinyl chloride, any one material in polypropylene.
Electrode layer is by electrochemical working electrode 2, electrochemistry to electrode 6, trigger electrode 3, connection electrode 5 and impedance tag slot
The length L in domain 4, width w or the gold electrode of thickness d composition, and according to the distance on the top at end of sucking blood by closely to remote sequence
It is successively electrochemical working electrode 2, electrochemistry to electrode 6, trigger electrode 3 and connects electrode 5, connects electrode 5 and be arranged in electrode
At the insertion end of layer, electrode 5 and electrochemistry are being connected to one gold electrode region of setting, impedance identified areas 4 between electrode 6
The length L of electrode, width w or thickness d are modulated.
There is stomata 10 in 11 upper end of coating.
Test strips of the invention, which are pre-seted, measures concentration curve, the examination of every batch of corresponding to the electric current vs of different batches test paper
Paper can determine the test of electrode 6 mark impedance by connecting electrode 5 and electrochemistry, control 4 electrode of impedance identified areas
Length L, width w or thickness d achieve the purpose that mark test paper batch, that is, connect electrode 5 and electrochemistry to electrode 6 it
Between impedance Z in can react the print batches of test paper.By measuring the impedance of test paper, the batch of test paper is determined, from corresponding
The test paper of corresponding batch is tested to call matched curve in the measurement concentration curve library electric current vs pre-seted in instrument, reaches
Automatically the purpose calibrated.
Application principle of the invention is further described with reference to the accompanying drawing.
Substrate layer 1: it uses polyethylene terephthalate (PET), polyvinyl chloride (PVC), one of polypropylene (PP).
Electrode layer: material selection gold is used.Electrochemical reaction cavity 8 respectively includes 1 electrode system, and electrode system includes
One electrochemical working electrode 2, an electrochemistry connect electrode 5 and an impedance with one to electrode 6, a trigger electrode 3
Identified areas 4.Electrochemical working electrode 2 and electrochemistry measure electrode 6 to the intracorporal blood sample of chamber, finally corresponding
Tester on show the concentration measurement of this measured object.
Trigger electrode 3 is located at the bottom of electrochemical reaction cavity 8, must reach after blood is from top into end of sucking blood
The trigger electrode test paper of bottom can just issue measurement countdown to corresponding tester, and tester starts countdown test, triggering
The design of electrode can not fill the inaccuracy of measured value caused by entire reaction zone to avoid due to blood；It is one of to connect electricity
Pole 5 is located at the bottom of test paper, when test paper is inserted into corresponding tester, connection electrode and two of disconnecting circuit in combined measuring instrument
End is connected so that the circuit of test paper circuit and tester constitutes complete circuit, instrument will Auto Power On, taken out after being measured
Test strips out, instrument will automatic shutdowns.The impedance of impedance identified areas 4 the length L by 4 electrode of impedance identified areas, width w
Or thickness d is modulated, according to different test paper batches, (such as A batch is tried for the impedance different to impedance tag slot laser ablation
Paper, etching impedance tag slot to connect electrode 5 and electrochemistry to the similarly B batch test paper of the impedance Z in=Z1 between electrode 6,
Etching impedance tag slot to connect electrode 5 and electrochemistry to the impedance Z in=Z2 between electrode 6；C batch test paper, etching resistance
Anti- tag slot to connect electrode 5 and electrochemistry to the impedance Z in=Z3 between electrode 6；) enzyme layer 9, it is anti-to provide generation electrochemistry
The enzyme answered.Coating 11: being used to covering part electrode layer, and there is a stomata 10 in the front end of the coating 11, for be discharged blood into
Enter air when siphon passage, wherein the part of the unlapped electrode layer of stomata 10 forms the reaction zone with end open at one end.
Spacer layer 12: being positioned over the upper end of layer 11, for protecting reaction zone not by the interference of external environment.
Application effect of the invention is explained in detail below with reference to test.
Testing impedance: matching impedance is worth corresponding concentration c VS electric current i curve graph: the corresponding song such as test paper batch 1,2,3
Line 1, curve 2, curve 3.Fig. 2 has reacted the relation curve of measured object concentration Yu surveyed current concentration.Example: curve 1 represents A batch
Test paper, the Z of A batch is made by the impedance of 1 middle impedance identified areas 4 of control figurein=Z1, pass through apparatus measures ZinIf
Zin=Z1, the curve of the measurement electric current VS measurement concentration of pre-stored corresponding A batch test paper in instrument is called to match the examination of A batch
Paper, and so on B batch (Zin=Z2), C batch (Zin=Z3) etc..
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (7)
1. it is a kind of with impedance identification electrochemistry test paper, which is characterized in that it is described with impedance identification electrochemistry test paper by down toward
On be successively bonded substrate layer, an electrochemical reaction cavity, enzyme layer, coating and the spacer layer for protecting coating upper end.
2. as described in claim 1 with impedance identification electrochemistry test paper, which is characterized in that the electrode layer be gold electrode or
Person's carbon silver electrode.
3. the electrochemistry test paper as described in claim 1 with impedance identification, which is characterized in that the substrate layer is using poly- to benzene
Dicarboxylate, polyvinyl chloride, any one material in polypropylene.
4. the electrochemistry test paper as claimed in claim 2 with impedance identification, which is characterized in that the electrode layer is by electrochemistry work
Make electrode, electrochemistry to electrode, trigger electrode, the gold electrode composition for connecting electrode and impedance identified areas；
According to end of sucking blood top distance by closely to remote sequence be successively electrochemical working electrode, electrochemistry to electrode, touching
Power generation pole with connect electrode.
5. the electrochemistry test paper as claimed in claim 4 with impedance identification, which is characterized in that the connection electrode is arranged in electricity
At the insertion end of pole layer, electrode and electrochemistry are being connected to one gold electrode region of setting between electrode.
6. the electrochemistry test paper as described in claim 1 with impedance identification, which is characterized in that there is gas in the coating upper end
Hole.
7. a kind of application method of the electrochemistry test paper as described in claim 1 with impedance identification, which is characterized in that the band resistance
The application method of the electrochemistry test paper of anti-identification includes: the test paper of every batch of by connecting electrode and electrochemistry to electrode test mark
Impedance is known to determine, controls the length L of impedance identified areas electrode, width w or thickness d to reach the mesh of label test paper batch
's；Electrode and electrochemistry are connected to the print batches of the impedance Z in reaction test paper between electrode；By measuring the impedance of test paper,
The batch for determining test paper, the electric current vs pre-seted in corresponding instrument measure concentration curve library to call matched curve
Test the test paper of corresponding batch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811122664.XA CN109142488A (en) | 2018-09-26 | 2018-09-26 | A kind of electrochemistry test paper and its application method with impedance identification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811122664.XA CN109142488A (en) | 2018-09-26 | 2018-09-26 | A kind of electrochemistry test paper and its application method with impedance identification |
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|---|---|
| CN109142488A true CN109142488A (en) | 2019-01-04 |
Family
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| CN201811122664.XA Pending CN109142488A (en) | 2018-09-26 | 2018-09-26 | A kind of electrochemistry test paper and its application method with impedance identification |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110327057A (en) * | 2019-07-30 | 2019-10-15 | 三诺生物传感股份有限公司 | A kind of multiple determination strip |
| CN112285171A (en) * | 2020-11-25 | 2021-01-29 | 三诺生物传感股份有限公司 | Impedance testing method applied to electrochemical test strip and electrochemical measuring system |
| CN113588935A (en) * | 2021-07-12 | 2021-11-02 | 成都云芯医联科技有限公司 | Electrochemical bar-code-free blood glucose test paper and preparation method thereof |
| CN113884686A (en) * | 2021-10-18 | 2022-01-04 | 四川成电医联科技咨询有限公司 | Siphon type total homocysteine test paper and manufacturing method thereof |
| CN114778631A (en) * | 2022-06-17 | 2022-07-22 | 北京华益精点生物技术有限公司 | Body index detection device and method |
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Application publication date: 20190104 |