CN109136079B - Bacteria stock solution processing device for detecting and analyzing bacterial drug resistance and using method - Google Patents

Abstract

The invention discloses a bacterium stock solution processing device for detecting and analyzing bacterial drug resistance and a using method thereof, and mainly relates to the technical field of bacterium stock solution processing. Comprises a wall hanging bracket, an antibiotic injector, an adjustable pedestal and a bacteria stock solution processing box; the strain stock solution processing box comprises a uniformly mixing box and a large equal dividing box, wherein a plurality of small equal dividing boxes are arranged in the large equal dividing box, the small equal dividing boxes are arranged in the inner cavity of the large equal dividing box in a specification mode, and a plurality of testing cavities are arranged in the small equal dividing boxes in an equal dividing mode. The invention has the beneficial effects that: a plurality of culture mediums with the same conditions can be manufactured at one time, each antibiotic detection corresponds to one small aliquot box, and the drug resistance detection of various antibiotics can be completed by one-time culture; and each small halving box is internally provided with a plurality of test cavities, so that comparison and averaging are facilitated, the limitation in the prior art is well solved, and the rapid detection on the quality of livestock products is facilitated.

Description

Bacteria stock solution processing device for detecting and analyzing bacterial drug resistance and using method

Technical Field

The invention relates to the technical field of bacteria stock solution treatment, in particular to a bacteria stock solution treatment device for detecting and analyzing bacterial drug resistance and a using method thereof.

Background

Due to its broad antibiotic-killing ability, antibiotics have been commonly used in livestock and poultry farming and meat product processing worldwide since the 50 s of the 20 th century, in addition to the treatment of human diseases. The long-term abuse of veterinary antibiotics in animal husbandry and breeding causes the ubiquitous presence of drug-resistant bacteria in animal products, which becomes an important influencing factor for food safety. Once resistance occurs, the therapeutic effect of the drug is significantly reduced. The problem of bacterial resistance has become one of the focus of global attention, and almost every effective antibiotic has found the corresponding resistant bacteria. The generation of bacterial drug resistance not only causes the control capability of the drug on livestock breeding diseases to be greatly weakened, but also has the potential risk of transmitting drug resistance to human pathogenic bacteria, and the importance of bacterial drug resistance detection work in animal products is increasingly shown.

The prior art of detecting the drug resistance of bacteria is to culture bacteria in culture media containing different antibiotics by utilizing the traditional bacterial culture technology and directly observe the inhibition of the antibiotics on the growth of the bacteria, generally, the growth can be detected within 24 hours or more, simultaneously, each antibiotic is independently added into the culture media (the simultaneous addition of more than two antibiotics can generate a synergistic effect or an antagonistic effect), in addition, in order to facilitate the comparison and the average value of each antibiotic, a plurality of culture media are needed for testing, and a plurality of times of culture are needed for determining the drug resistance of one strain to a plurality of antibiotics, so the traditional method has great limitation and is not beneficial to the rapid detection of the quality of animal products.

Disclosure of Invention

The invention aims to provide a bacteria stock solution processing device for detecting and analyzing bacterial drug resistance and a using method thereof, which can be used for preparing a plurality of culture mediums with the same conditions at one time, each antibiotic detection corresponds to a small aliquot box, and the drug resistance detection of a plurality of antibiotics can be completed by carrying out one-time culture; and each small halving box is internally provided with a plurality of test cavities, so that comparison and averaging are facilitated, the limitation in the prior art is well solved, and the rapid detection on the quality of livestock products is facilitated.

In order to achieve the purpose, the invention is realized by the following technical scheme:

a fungus stoste processing apparatus for bacterial drug resistance detection and analysis, including hanging support, antibiotic syringe, adjustable pedestal and fungus stoste processing box; the antibiotic injectors are detachably arranged on the wall-mounted bracket in a specification; the adjustable pedestal is arranged at the bottom of the antibiotic injector; the fungus stoste processing box includes mixing box and big halving box, mixing box and big halving box can sealed block, be equipped with a plurality of little halving boxes in the big halving box, it is a plurality of the box specification of little halving is arranged and is set up at big halving box inner chamber, the interior halving of little halving box is equipped with a plurality of test chambers.

The fungus stoste processing box is transparent glass material, be equipped with drawing liquid mouth and stabilizer blade on the mixing box.

The adjustable pedestal comprises a sliding table and a sliding seat, wherein a sliding groove is formed in the sliding table, the sliding seat is connected with the sliding table in a sliding mode through the sliding groove, and the small halving box is connected with the top of the sliding seat in a clamping mode.

The bottom of the antibiotic injector is clamped with an injection joint, and the number and the position of the liquid outlet heads on the injection joint are adapted to the test cavities on the small halving boxes.

The adjustable table is characterized by further comprising an adjustable table, the adjustable table seats are fixedly arranged on the top of the adjustable table, and adjustable table legs are arranged at the bottom of the adjustable table.

The adjustable table legs comprise fixed legs, telescopic legs and limiting devices, the fixed legs are fixed on the test detection table, first spring cavities are formed in the fixed legs, a plurality of clamping holes are formed in the side portions of the fixed legs, adjusting springs are arranged in the first spring cavities, the top ends of the telescopic legs are fixed at the bottom of the adjustable table, and the bottom ends of the telescopic legs extend into the spring cavities to be in contact with the top ends of the adjusting springs; stop device includes reset spring and bayonet lock, be equipped with second spring chamber on the flexible leg, reset spring sets up in second spring intracavity, bayonet lock inner stretches into second spring intracavity and reset spring contact, the bayonet lock outer end is spacing with the joint of card hole.

And iron sheets are arranged on the outer edge of the top surface of the small halving box.

The use method of the bacteria stock solution processing device for detecting and analyzing the bacterial drug resistance comprises the following steps:

s1, installing a wall hanging support and antibiotic injectors, wherein two sides of the wall hanging support are fixed on a wall body of a laboratory through screws, a clamping seat is arranged on the wall hanging support, the antibiotic injectors are fixed on the wall hanging support through the clamping seat, the distance between every two adjacent antibiotic injectors is equal, different antibiotics are installed in each antibiotic injector, and an injection joint is installed at the bottom of each antibiotic injector;

s2, adjusting the height of the adjustable table, clamping one of the small equal division boxes on the top of the sliding seat, placing the small equal division box under the injection joint, and then adjusting the height of the adjustable table legs to enable the distance between the top surface of the small equal division box and the injection joint to be 0.5 cm-1.5 cm;

s3, sliding the sliding seat to the left side of the sliding table along the sliding groove, so that the sliding seat is not opposite to the injection joint;

s4, arranging the small equally dividing boxes into large equally dividing boxes in specification, and then hermetically clamping the uniformly mixing box and the large equally dividing boxes;

s5, injecting the bacteria stock solution into the bacteria stock solution processing box through the solution extraction port until the liquid level of the bacteria stock solution is higher than the top surface of the small aliquot box;

s6, blocking the liquid extraction port, turning over the bacteria stock solution processing box to enable the bacteria stock solution to be contained in the mixing box to be mixed uniformly, separating the mixing box and the large dividing box to stand for half an hour, and measuring the concentration of bacteria in the bacteria stock solution; if the bacteria concentration meets the detection requirement, the blending box and the large equally-dividing box are clamped in a sealing manner, the bacteria stock solution processing box is turned over, and the bacteria stock solution is evenly divided and poured into the test cavity of the small equally-dividing box; if the bacterial concentration does not meet the detection requirement, continuing standing for 10 minutes, and measuring the bacterial concentration in the bacterial stock solution every 10 minutes until the bacterial concentration meets the detection requirement; after the bacteria concentration meets the detection requirement, the blending box and the large equal division box are clamped in a sealing mode, the bacteria stock solution processing box is turned over, and the bacteria stock solution is evenly poured into the test cavity of the small equal division box;

s7, pumping the redundant bacteria stock solution in the bacteria stock solution processing box from the solution pumping port until the bacteria stock solution just overflows the top surface of the small aliquot box; then, extracting the bacteria stock solution in each test cavity by using an injector for 0.3-0.5 cm³So that the fungus stock solution in each test cavity is not full;

s8, lifting each small equal division box onto a sliding seat by using a magnetic lifting handle, and sliding the sliding seat to the right side of the sliding table along the sliding groove to enable the sliding seat to face the injection joint;

s9, then injecting different antibiotics into different sub-cartridges by using an antibiotic injector and an injection joint on the wall-mounted bracket, and injecting the same antibiotic into each test cavity;

and S10, finishing the treatment of the strain stock solution, and standing for the next nucleic acid extraction.

Compared with the prior art, the invention has the beneficial effects that:

nine small equal dividing boxes can be arranged in the mixing box and the large equal dividing box of the device, a plurality of culture mediums with the same conditions can be manufactured at one time, each antibiotic detection corresponds to one small equal dividing box, and the drug resistance detection of various antibiotics can be completed by one-time culture; and nine test cavities are arranged in each small halving box, and the same antibiotics are injected into the nine test cavities, so that comparison and averaging are facilitated, the limitation in the prior art is well solved, and the quality of livestock products can be rapidly detected.

Drawings

FIG. 1 is a schematic diagram of the detailed structure of the present invention.

FIG. 2 is an enlarged view of portion I of FIG. 1 according to the present invention.

FIG. 3 is an enlarged view of section II of FIG. 2 of the present invention.

FIG. 4 is a schematic structural view of the separated state of the fungus stock solution processing box of the present invention.

FIG. 5 is a state diagram of the bacteria stock solution processing box when the bacteria stock solution is contained in the mixing box and mixed evenly.

FIG. 6 is a state diagram of a bacteria stock solution processing box when the bacteria stock solution is contained in a mixing box and mixed evenly and then the redundant bacteria stock solution in the bacteria stock solution processing box is extracted.

FIG. 7 is a schematic diagram of the mixing box of the present invention.

FIG. 8 is a schematic diagram of a large aliquot box according to the present invention.

FIG. 9 is a schematic view of a small aliquot box according to the present invention.

Fig. 10 is a schematic view of the magnetic handle structure of the present invention.

Reference numerals shown in the drawings:

1. a wall-mounted support; 2. an antibiotic injector; 3. an adjustable pedestal; 4. a fungus stock solution processing box; 5. a blending box; 6. dividing the box into equal parts; 7. dividing the box into small equal parts; 8. a test chamber; 9. a liquid extraction port; 10. a support leg; 11. a sliding table; 12. a slide base; 13. a chute; 14. an injection fitting; 15. an adjustable table; 16. the table legs can be adjusted; 17. a fixed leg; 18. a telescopic leg; 19. a test detection table; 20. a first spring chamber; 21. a clamping hole; 22. adjusting the spring; 23. a return spring; 24. a bayonet lock; 25. a second spring chamber; 26. iron sheets; 27. a card holder; 28. magnetic handle.

Detailed Description

The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the present application.

The invention relates to a bacteria stock solution processing device for detecting and analyzing bacterial drug resistance and a using method thereof, the main structure of which comprises a wall hanging bracket 1, an antibiotic injector 2, an adjustable pedestal 3 and a bacteria stock solution processing box 4; the antibiotic injectors 2 are detachably arranged on the wall-mounted support 1 in a specification, the wall-mounted support 1 is provided with a clamping seat 27, and the antibiotic injectors 2 are clamped on the wall-mounted support 1 through the clamping seat 27; the adjustable pedestal 3 is arranged at the bottom of the antibiotic injector 2; fungus stoste processing box 4 includes mixing box 5 and big halving box 6, mixing box 5 can seal the block with big halving box 6, be equipped with a plurality of little halving boxes 7 in the big halving box 6, it is a plurality of 7 specifications of little halving box are arranged and are set up in 6 inner chambers of big halving box, the halving is equipped with a plurality of test chamber 8 in the little halving box 7.

The bacteria stock solution processing box 4 is made of transparent glass materials, and the transparent glass materials are convenient for observing the bacteria stock solution in the bacteria stock solution processing box 4; the blending box 5 is provided with a liquid extraction port 9 and support legs 10, the bacteria stock solution is injected from the liquid extraction port 9, and the redundant bacteria stock solution after blending is extracted from the liquid extraction port 9.

Adjustable pedestal 3 includes slip table 11 and slide 12, be equipped with spout 13 on the slip table 11, slide 12 passes through spout 13 and slip table 11 sliding connection, little halving box 7 and slide 12 top joint. Sliding the slide 12 along the slide channel 13 to the left of the slide table 11, so that the slide 12 no longer faces the injection connector 14, thus facilitating the use of the magnetic handle 28 to lift each small aliquot box 7 onto the slide 12; the carriage 12 is then slid along the slide channel 13 to the right of the slide table 11 with the carriage 12 facing the injection sub 14.

The bottom of the antibiotic injector 2 is clamped with an injection joint 14, and the number and the position of liquid outlet heads on the injection joint 14 are adapted to the test cavity 8 on the small partition box 7. This enables a single injection to fill all test chambers 8 with antibiotic.

The adjustable table is characterized by further comprising an adjustable table 15, the adjustable pedestals 3 are fixedly arranged at the top of the adjustable table 15, and adjustable table legs 16 are arranged at the bottom of the adjustable table 15. The height of the adjustable table 15 can be adjusted by adjusting the height of the adjustable table legs 16, so that the distance between the top surface of the small aliquoting box 7 and the injection connector 14 is 0.5 cm-1.5 cm.

The adjustable table legs 16 comprise fixed legs 17, telescopic legs 18 and limiting devices, the fixed legs 17 are fixed on a test detection table 19, first spring cavities 20 are formed in the fixed legs 17, a plurality of clamping holes 21 are formed in the side portions of the fixed legs 17, adjusting springs 22 are arranged in the first spring cavities 20, the top ends of the telescopic legs 18 are fixed at the bottom of the adjustable table 15, and the bottom ends of the telescopic legs 18 extend into the spring cavities to be in contact with the top ends of the adjusting springs 22; stop device includes reset spring 23 and bayonet lock 24, be equipped with second spring chamber 25 on the flexible leg 18, reset spring 23 sets up in second spring chamber 25, bayonet lock 24 the inner contact with reset spring 23 in stretching into second spring chamber 25, bayonet lock 24 outer end is spacing with the joint of card hole 21.

The outer edge of the top surface of the small partition box 7 is provided with an iron sheet 26. Each small portion 7 is lifted onto the slide 12 by means of magnetic force between the magnetic handle 28 and the iron plate 26 using the magnetic handle 28.

The use method of the bacteria stock solution processing device for detecting and analyzing the bacterial drug resistance comprises the following steps:

s1, installing a wall hanging support 1 and antibiotic injectors 2, fixing two sides of the wall hanging support 1 on a wall of a laboratory through screws, arranging clamping seats 27 on the wall hanging support 1, fixing the antibiotic injectors 2 on the wall hanging support 1 through the clamping seats 27, enabling the distances between every two adjacent antibiotic injectors 2 to be equal, installing different antibiotics in each antibiotic injector 2, and installing an injection joint 14 at the bottom of each antibiotic injector 2;

s2, adjusting the height of the adjustable table 15, clamping one of the small halving boxes 7 on the top of the sliding seat 12, placing the small halving box 7 under the injection joint 14, and then adjusting the height of the adjustable table leg 16 to enable the distance between the top surface of the small halving box 7 and the injection joint 14 to be 0.5 cm-1.5 cm;

s3, sliding the sliding seat 12 to the left side of the sliding table 11 along the sliding groove 13, so that the sliding seat 12 is no longer opposite to the injection joint 14;

s4, arranging the small equal division boxes 7 into the large equal division boxes 6 in specification, and then hermetically clamping the uniform mixing box 5 and the large equal division boxes 6;

s5, injecting the bacteria stock solution into the bacteria stock solution processing box 4 through the solution drawing port 9 until the liquid level of the bacteria stock solution is higher than the top surface of the small aliquot box 7;

s6, blocking the liquid extraction port 9, turning over the bacteria stock solution processing box 4 to enable the bacteria stock solution to be contained in the mixing box 5 to be mixed uniformly, separating the mixing box 5 and the large grading box 6 to stand for half an hour, and measuring the concentration of bacteria in the bacteria stock solution; if the bacteria concentration meets the detection requirement, the blending box 5 and the large equal-dividing box 6 are clamped in a sealing mode, the bacteria stock solution processing box 4 is turned over, and the bacteria stock solution is evenly divided and poured into the test cavity 8 of the small equal-dividing box 7; if the bacterial concentration does not meet the detection requirement, continuing standing for 10 minutes, and measuring the bacterial concentration in the bacterial stock solution every 10 minutes until the bacterial concentration meets the detection requirement; after the bacteria concentration meets the detection requirement, the blending box 5 and the large equal-dividing box 6 are clamped in a sealing way, the bacteria stock solution processing box 4 is turned over, and the bacteria stock solution is evenly divided and poured into the test cavity 8 of the small equal-dividing box 7;

s7, pumping redundant bacteria stock solution in the bacteria stock solution processing box 4 from the solution pumping port 9 until the bacteria stock solution just flows over the top surface of the small halving box 7; then, a syringe is used for extracting the bacteria stock solution in each test cavity 8 by 0.3-0.5 cm³So that the bacteria stock solution in each test cavity 8 is not full;

s8, lifting each small equal-division box 7 onto the sliding seat 12 by using the magnetic handle 28, and sliding the sliding seat 12 to the right side of the sliding table 11 along the sliding groove 13, so that the sliding seat 12 faces the injection joint 14;

s9, then injecting different antibiotics into different small aliquot boxes 7 by using the antibiotic injector 2 and the injection joint 14 on the wall-mounted support 1, and injecting the same antibiotics into each test cavity 8;

and S10, finishing the treatment of the strain stock solution, and standing for the next nucleic acid extraction.

By the device and the using method, a plurality of culture mediums with the same conditions can be manufactured at one time, each antibiotic detection corresponds to one small aliquot box 7, and the drug resistance detection of various antibiotics can be completed by one-time culture; and each small halving box 7 is internally provided with a plurality of testing cavities 8, so that comparison and averaging are facilitated, the limitation in the prior art is well solved, and the quality of livestock products can be rapidly detected.

Claims (4)

1. A fungus stoste processing apparatus for bacterium drug resistance detection analysis which characterized in that: the antibiotic treatment device comprises a wall hanging support (1), an antibiotic injector (2), an adjustable pedestal (3) and a bacteria stock solution treatment box (4), wherein the adjustable pedestal (3) comprises a sliding table (11) and a sliding seat (12), the sliding table (11) is provided with a sliding groove (13), and the sliding seat (12) is in sliding connection with the sliding table (11) through the sliding groove (13);

the antibiotic injectors (2) are detachably arranged on the wall-mounted support (1) in a specification and in a detachable mode, injection joints (14) are clamped at the bottoms of the antibiotic injectors (2), and the number and the positions of liquid outlet heads on the injection joints (14) are matched with those of test cavities (8) on the small halving boxes (7);

the adjustable pedestals (3) are arranged at the bottom of the antibiotic injector (2), the antibiotic injector further comprises an adjustable table (15), the adjustable pedestals (3) are fixedly arranged at the top of the adjustable table (15), and adjustable table legs (16) are arranged at the bottom of the adjustable table (15);

fungus stoste processing box (4) are including mixing box (5) and big halving box (6), mixing box (5) can seal up the block with big halving box (6), be equipped with a plurality of little halving boxes (7) in big halving box (6), and is a plurality of little halving box (7) specification is arranged and is set up at big halving box (6) inner chamber, the halving is equipped with a plurality of test chamber (8) in little halving box (7), little halving box (7) top surface outer fringe is equipped with iron sheet (26), little halving box (7) and slide (12) top joint.

2. The apparatus for processing a bacterial stock solution for use in the detection and analysis of bacterial drug resistance according to claim 1, wherein: the fungus stock solution processing box (4) is made of transparent glass, and a liquid drawing port (9) and support legs (10) are arranged on the blending box (5).

3. The apparatus for processing a bacterial stock solution for use in the detection and analysis of bacterial drug resistance according to claim 1, wherein: the adjustable table legs (16) comprise fixed legs (17), telescopic legs (18) and limiting devices, the fixed legs (17) are fixed on a test detection table (19), first spring cavities (20) are formed in the fixed legs (17), a plurality of clamping holes (21) are formed in the side portions of the fixed legs (17), adjusting springs (22) are arranged in the first spring cavities (20), the top ends of the telescopic legs (18) are fixed to the bottom of the adjustable table (15), and the bottom ends of the telescopic legs (18) extend into the spring cavities to be in contact with the top ends of the adjusting springs (22); stop device includes reset spring (23) and bayonet lock (24), be equipped with second spring chamber (25) on flexible leg (18), reset spring (23) set up in second spring chamber (25), bayonet lock (24) inner is stretched into in second spring chamber (25) and is contacted with reset spring (23), bayonet lock (24) outer end is spacing with calorie hole (21) joint.

4. The method of using a bacterial bulk fluid processing apparatus for bacterial drug resistance detection assays according to any of claims 1-3, wherein:

s1, installing a wall hanging support (1) and antibiotic injectors (2), fixing two sides of the wall hanging support (1) on a wall of a laboratory through screws, arranging clamping seats (27) on the wall hanging support (1), fixing the antibiotic injectors (2) on the wall hanging support (1) through the clamping seats (27), enabling the distances between every two adjacent antibiotic injectors (2) to be equal, installing different antibiotics in each antibiotic injector (2), and installing an injection joint (14) at the bottom of each antibiotic injector (2);

s2, adjusting the height of the adjustable table (15), clamping one small halving box (7) at the top of the sliding seat (12), placing the small halving box (7) under the injection joint (14), and then adjusting the height of the adjustable table legs (16) to enable the distance between the top surface of the small halving box (7) and the injection joint (14) to be 0.5 cm-1.5 cm;

s3, sliding the sliding seat (12) to the left side of the sliding table (11) along the sliding groove (13) so that the sliding seat (12) is not opposite to the injection joint (14);

s4, arranging the small equal-division boxes (7) into the large equal-division boxes (6) in a specification mode, and then hermetically clamping the uniform mixing box (5) and the large equal-division boxes (6);

s5, injecting the strain stock solution into the strain stock solution processing box (4) through the solution pumping port (9) until the level of the strain stock solution is higher than the top surface of the small halving box (7);

s6, blocking the liquid suction port (9), turning over the bacteria stock solution processing box (4) to enable the bacteria stock solution to be contained in the mixing box (5) to be mixed uniformly, separating the mixing box (5) and the large grading box (6) to stand for half an hour, and measuring the concentration of bacteria in the bacteria stock solution; if the bacteria concentration meets the detection requirement, the blending box (5) and the large equal-dividing box (6) are clamped in a sealing way, the bacteria stock solution processing box (4) is turned over, and the bacteria stock solution is evenly divided and poured into the test cavity (8) of the small equal-dividing box (7); if the bacterial concentration does not meet the detection requirement, continuing standing for 10 minutes, and measuring the bacterial concentration in the bacterial stock solution every 10 minutes until the bacterial concentration meets the detection requirement; after the bacteria concentration meets the detection requirement, the blending box (5) and the large equal-dividing box (6) are clamped in a sealing way, the bacteria stock solution processing box (4) is turned over, and the bacteria stock solution is evenly divided and poured into the test cavity (8) of the small equal-dividing box (7);

s7, pumping redundant bacteria stock solution in the bacteria stock solution processing box (4) from the solution pumping port (9) until the bacteria stock solution just flows over the top surface of the small aliquot box (7); then, extracting the bacteria stock solution in each test cavity (8) by using an injector for 0.3-0.5 cm3, so that the bacteria stock solution in each test cavity (8) is not full;

s8, lifting each small halving box (7) onto the sliding seat (12) by using the magnetic handle (28), and sliding the sliding seat (12) to the right side of the sliding table (11) along the sliding groove (13) to enable the sliding seat (12) to be opposite to the injection joint (14);

s9, then injecting different antibiotics into different small aliquoting boxes (7) by using an antibiotic injector (2) and an injection joint (14) on the wall-mounted support (1), and injecting the same antibiotics into each test cavity (8);

and S10, finishing the treatment of the strain stock solution, and standing for the next nucleic acid extraction.

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