CN109112171A - A kind of preparation method of the antibacterial substance based on marine microorganism - Google Patents
A kind of preparation method of the antibacterial substance based on marine microorganism Download PDFInfo
- Publication number
- CN109112171A CN109112171A CN201811139541.7A CN201811139541A CN109112171A CN 109112171 A CN109112171 A CN 109112171A CN 201811139541 A CN201811139541 A CN 201811139541A CN 109112171 A CN109112171 A CN 109112171A
- Authority
- CN
- China
- Prior art keywords
- preparation
- substance based
- bacterial strain
- marine microorganism
- antibacterial substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a kind of preparation methods of antibacterial substance based on marine microorganism, specifically includes the following steps: S1, bacterial strain isolate and purify: having obtained single aspergillus fungi；S2, fermented and cultured；The extraction of S3, bacterial strain secondary metabolite；The purifying of S4, bacterial strain secondary metabolite, isolated 2 antibacterial active compounds A and B；It is tested through bacteriostatic activity, compound A and B shows good bacteriostatic activity to Escherichia coli, staphylococcus aureus, bacillus subtilis；It is tested through anti-tumor activity, it was found that the effective concentration IC50 of compound A, B, which are respectively 16.6 μM, 18.2 μM, shows anti-tumor activity, there is inhibitory activity to including lactiferous ducts cancer cell T-47D, human breast cancer cell line Bcap-37, human liver cancer cell SMC-7721, cervical cancer cell Hela, human lung cancer cell A549.
Description
Technical field
The invention belongs to biopharmaceutical technologies, and in particular to a kind of antibacterial substance based on marine microorganism
Preparation method.
Background technique
In past over half a century, due to being widely used for antibiotic, especially unreasonable clinical misuse is caused
Drug resistance pathogenic bacteria type is continuously increased, and brings great threat to human life and health.In order to reduce the production of bacterial drug resistance
It is raw, it is badly in need of the new quick antibacterials of height of exploitation, to improve the validity of clinical application.
Marine microorganism has a very wide distribution, and species diversity is abundant, and growth cycle is short, and metabolism is easy to regulate and control, and strain is easier to
The features such as breeding is with that can realize industrialized production with large scale fermentation, and the exploitation of marine microorganism is unlikely to lead to marine species
Unbalance with ocean habitat, with more the sustainable use of natural resources, the conditions such as ocean is with high salt, high pressure, low illumination make ocean
Microorganism can generate a large amount of structure novels, the unique secondary metabolite of activity, and many compounds have antibacterial activity,
Important sources are provided to find potential antibacterials lead compound.
Ocean is a huge treasure-house.There are a large amount of lead compounds to separate from marine resources now, they
With various bioactivity, such as: antitumor, antibacterial, pest-resistant, anti-pollution is turbid.Ocean is also nourished a large amount of undiscovered simultaneously
Microorganism, they are the potential producers of active secondary metabolite.Fungi is the weight of bioactive natural product and original new drug
Want source.With deepening continuously to fungal secondary metabolite research, compare in the past few years, it is reported to be separated from fungi
The quantity rapid development of obtained bioactive natural product.But from integral level, compound go out new rate but slightly have under
Drop, the probability for repeating research are constantly rising.Thus it is possible to no isolated with remarkable activity and novel structure from fungi
Natural products, other than separation means, the diversity of sample collecting location, the separation of bacterial strain and screening means and bacterial strain training
Feeding base composition and training method have all served vital.
In recent years, with the pharmaceutical chemical development of marine natural, more and more antibacterial active compounds are from the micro- life in ocean
Constantly separation obtains in object, provides material base to find antibacterial agent.Using the method for artificial cultivation and fermentation, from ocean
The secondary metabolite for having important antibacterial activity is obtained in microorganism, has the characteristics that environmental-friendly, sustainable development, it can be effective
The critical problems such as the medicine source in drug discovery process are solved, are had great importance.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation methods of antibacterial substance based on marine microorganism, obtain
The antibacterial substance A and B of novel structure, are tested through bacteriostatic activity, and antibacterial substance A and B have good bacteriostatic activity
And anti-tumor activity.
Present invention technical problem to be solved are as follows:
(1) single fungi strain how is isolated and purified out；
(2) antibacterial substance of novel structure how is obtained；
(3) bioactivity of isolated compound how is evaluated.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of preparation method of the antibacterial substance based on marine microorganism, specifically includes the following steps:
S1, bacterial strain isolate and purify
It takes the Indian Ocean 10g halmeic deposit sample in the test tube of sterilizing, 100mL antiseptic sea water is added, oscillation mixes
To homogenate, this is 10-1Dilution takes turns doing gradient dilution in this way, obtains 10-2、10-3、10-4Dilution is successively drawn
The seawater of each different dilutions of 100 μ L is put in solid medium tablets, is uniformly coated on liquid with sterile spreading rod whole
A planar surface is subsequently placed in 20 DEG C of incubators and cultivates 4-7 days；The fungi with single bacterium colony is chosen from solid medium
Mycelium, using plate streak, one ring hypha,hyphae of picking or spore are crossed under 5-6 in new planar surface, are then inverted in
7 days are cultivated in 20 DEG C of incubators to get aspergillus fungi has been arrived；By purified aspergillus fungi strain inoculated in inclined-plane solid
In culture medium conservation pipe, saved backup after aspergillus fungi bacterium colony grows up to；
S2, fermented and cultured
By aspergillus fungi bacterial strain on from conservation pipe inclined plane inoculating to fungi liquid culture medium plate, cultivated 7 days in 26 DEG C
As seed plate, 30 are inoculated into transfer needle picking fungus colony from liquid plate culture medium and contains 400ml fermented fungal
In the 1000ml conical flask of fluid nutrient medium, fermented and cultured 40 days under 26 DEG C, standing non-illuminated conditions, fermented and cultured is obtained
Liquid；
The extraction of S3, bacterial strain secondary metabolite
The obtained fermentation culture of step S2 is filtered with gauze, filtrate is extracted three times with isometric ethyl acetate, is obtained
It ferments thick immersion liquid；Mycelium filter residue first uses 75% EtOH Sonicate to extract, after resulting extracting solution is concentrated into no ethyl alcohol, then with etc.
The ethyl extraction of volume three times, obtains the thick immersion liquid of mycelium, the thick immersion liquid that will ferment merges with the thick immersion liquid of mycelium to get arriving
Bacterial strain secondary metabolite crude extract；
The purifying of S4, bacterial strain secondary metabolite
Silica gel is added into bacterial strain secondary metabolite crude extract, crosses Rp-18 reverse phase silica gel column after mixing, uses stone
Oily ether: ethyl acetate gradient collects each eluent, is concentrated under reduced pressure, merges, isolated 2 antibacterial activity chemical combination
Object A and B；
The structural formula of the antibacterial active compounds A and B are as follows:
Further, the solid medium are as follows: wheat flour 17g, soluble starch 5g, glucose 3g, peptone 3g, fine jade
Rouge 20g, chloramphenicol 0.1g, antiseptic sea water 1L, each component after mixing, use pH adjusting agent adjust solid medium pH for
7.0-7.5。
Further, the potassium dihydrogen phosphate aqueous solution that the pH adjusting agent is 20%.
Further, in step S1, after cultivating 7 days in incubator, if bacterium colony is impure, continue scribing line separation repeatedly, until
Until single bacterium colony.
Further, in step S1, aspergillus fungi bacterium bacterium colony, which grows up to, is placed on 0-3 DEG C of refrigerator preservation.
Further, the fluid nutrient medium are as follows: wheat flour 17g, yeast powder 2g, glucose 3g, peptone 3g, sterile sea
Water 1L, each component after mixing, use pH adjusting agent to adjust solid medium pH as 7.0-7.5.
Further, the potassium dihydrogen phosphate aqueous solution that the pH adjusting agent is 10%.
Further, the detailed process of gradient elution described in step S4 are as follows: petroleum ether: ethyl acetate=9:1 is used first,
Elution 3-4h is carried out, then with petroleum ether: ethyl acetate=1:1 obtains antibacterial active compounds A after collecting eluent concentration；
Continue with petroleum ether: ethyl acetate=1:7.5 obtains antibacterial active compounds B after collecting eluent concentration.
Beneficial effects of the present invention:
(1) it by the way that Indian Ocean halmeic deposit is carried out gradient dilution culture, chooses from solid medium with single
The fungal mycelium of bacterium colony, using plate streak, one ring hypha,hyphae of picking or spore are crossed under 5-6 in new planar surface,
Then it is inverted in 20 DEG C of incubators and cultivates 7 days, if bacterium colony is impure, continue scribing line separation repeatedly, be up to obtaining single bacterium colony
Only, it finally obtained single aspergillus fungi；
(2) aspergillus fungi obtains fermentation culture by fermented and cultured, and fermentation culture is filtered with gauze, filtrate and bacterium
Filament filter residue is first respectively adopted organic solvent and is extracted, and extraction efficiency is high, and the recovery rate of obtained secondary metabolite is high, most
Column gradient elution is crossed using silica gel afterwards, has obtained the antibacterial substance A and B of novel structure, through nuclear-magnetism and mass spectral characteristi, verifying
The structure of antibacterial substance A and B, so far, it is not yet found that patent or document report；
(3) it is tested through bacteriostatic activity, compound A and B is equal to Escherichia coli, staphylococcus aureus, bacillus subtilis
Show good bacteriostatic activity；It is tested through anti-tumor activity, the effective concentration IC50 of discovery compound A, B are respectively 16.6
μM, 18.2 μM show anti-tumor activity, to including that lactiferous ducts cancer cell T-47D, human breast cancer cell line Bcap-37, human liver cancer are thin
Born of the same parents SMC-7721, cervical cancer cell Hela, human lung cancer cell A549 have inhibitory activity.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
Embodiment 1
A kind of preparation method of the antibacterial substance based on marine microorganism, specifically includes the following steps:
S1, bacterial strain isolate and purify
It takes the Indian Ocean 10g halmeic deposit sample in the test tube of sterilizing, 100mL antiseptic sea water is added, oscillation mixes
To homogenate, this is 10-1Dilution takes turns doing gradient dilution in this way, obtains 10-2、10-3、10-4Dilution is successively drawn
The seawater of each different dilutions of 100 μ L is put in solid medium tablets, is uniformly coated on liquid with sterile spreading rod whole
A planar surface is subsequently placed in 20 DEG C of incubators and cultivates 7 days；The fungi bacterium with single bacterium colony is chosen from solid medium
Filament, using plate streak, then one ring hypha,hyphae of picking or spore are inverted in 20 DEG C under new planar surface scribing line 5
It is cultivated 7 days in incubator, if bacterium colony is impure, continues scribing line separation repeatedly, until obtaining the single bacterium colony of form and solid colour
Until to get having arrived aspergillus fungi；By purified aspergillus fungi strain inoculated in inclined-plane solid medium conservation pipe,
Bacterium colony, which grows up to, is placed on 3 DEG C of refrigerator preservations；
The solid medium are as follows:
Wheat flour 17g, soluble starch 5g, glucose 3g, peptone 3g, agar 20g, chloramphenicol 0.1g, antiseptic sea water
1L, each component after mixing, use 20% potassium dihydrogen phosphate aqueous solution to adjust solid medium pH as 7.2；
S2, fermented and cultured
By aspergillus fungi bacterial strain on from conservation pipe inclined plane inoculating to fungi liquid culture medium plate, cultivated 7 days in 26 DEG C
As seed plate, 30 are inoculated into transfer needle picking fungus colony from liquid plate culture medium and contains 400ml fermented fungal
In the 1000ml conical flask of fluid nutrient medium, fermented and cultured 40 days under 26 DEG C, standing non-illuminated conditions, fermented and cultured is obtained
Liquid；
The fluid nutrient medium are as follows:
Wheat flour 17g, yeast powder 2g, glucose 3g, peptone 3g, antiseptic sea water 1L, each component after mixing, use
It is 7.5 that 10% potassium dihydrogen phosphate aqueous solution, which adjusts solid medium pH,.
The extraction of S3, bacterial strain secondary metabolite
The obtained fermentation culture of step S2 is filtered with gauze, filtrate is extracted three times with isometric ethyl acetate, is obtained
It ferments thick immersion liquid；Mycelium filter residue first uses 75% EtOH Sonicate to extract, after resulting extracting solution is concentrated into no ethyl alcohol, then with etc.
The ethyl extraction of volume three times, obtains the thick immersion liquid of mycelium, the thick immersion liquid that will ferment merges with the thick immersion liquid of mycelium to get arriving
Bacterial strain secondary metabolite crude extract；
The purifying of S4, bacterial strain secondary metabolite
Silica gel is added into bacterial strain secondary metabolite crude extract, crosses Rp-18 reverse phase silica gel column after mixing, uses stone
Oily ether: ethyl acetate gradient collects each eluent, is concentrated under reduced pressure, merges, isolated 2 antibacterial activity chemical combination
Object A and B；
The detailed process of the gradient elution are as follows: use petroleum ether first: ethyl acetate=9:1 carries out elution 3-4h,
Then with petroleum ether: ethyl acetate=1:1 obtains antibacterial active compounds A after collecting eluent concentration；Continue with petroleum ether:
Ethyl acetate=1:7.5 obtains antibacterial active compounds B after collecting eluent concentration；
The structural formula of the antibacterial active compounds A and B are as follows:
The antibacterial active compounds A structural characterization data are as follows:
1H NMR(400MHz,CDCl3): δ 9.64 (s, 1H), 8.22 (s, 1H), 8.05 (s, 1H), 7.75 (t, J=
7.1Hz, 1H), 7.39 (d, J=8.1Hz, 1H), 7.24 (d, J=8.0Hz, 1H), 7.14 (t, J=8.5Hz, 1H), 6.51 (s,
1H),4.89(t,1H),3.11(d,1H),2.85(d,2H),2.52(m,1H),1.25(m,2H),1.11(d,3H),0.75(t,
3H)；
13C NMR(101MHz,CDCl3):δ175.9,168.8,165.4,161.1,153.4,150.5,146.7,
140.3,136.1,128.6,121.5,119.3,117.0,110.6,59.5,57.4,38.7,36.0,23.9,15.1,
12.6ppm；
HRMS m/z(ESI+)calcd for C21H21N3O5([M]+),395.1523,found 395.1503。
The antibacterial active compounds B structure characterize data is as follows:
1H NMR(400MHz,CDCl3): δ 9.71 (s, 1H), 8.23 (s, 1H), 8.02 (s, 1H), 7.86 (t, J=
7.1Hz, 1H), 7.41 (d, J=8.1Hz, 1H), 7.24 (d, J=8.0Hz, 1H), 7.14 (t, J=8.5Hz, 1H), 6.51 (s,
1H),4.89(t,1H),3.13(d,1H),2.88(d,2H),2.52(m,1H),1.25(m,2H),1.12(d,3H),0.77(t,
3H)；
13C NMR(101MHz,CDCl3):δ178.4,169.1,165.4,162.7,161.1,146.7,143.4,
140.3,136.1,127.6,125.5,119.3,117.0,110.6,59.5,57.4,38.7,36.5,23.4,15.0,
12.1ppm；
HRMS m/z(ESI+)calcd for C21H21N3O6([M]+),411.1435,found 411.1412。
Embodiment 2
Bacteriostatic activity test:
Using micro-dilution method measurement compound A, B to Escherichia coli, staphylococcus aureus, bacillus subtilis this three
The bacteriostatic activity of strain pathogenic bacteria, test result are as shown in Table 1；
Table one, compound A, B test structure to the inhibitory activity of bacterium
As shown in Table 1, compound A, B shows Escherichia coli, staphylococcus aureus, bacillus subtilis
Good bacteriostatic activity.
Embodiment 3
Anti-tumor activity experiment:
It is antitumor using mtt assay detection compound A, B to human milk glandular tube cancer cell T-47D, human breast cancer cell line Bcap-37,
Human liver cancer cell SMC-7721, cervical cancer cell Hela, human lung cancer cell A549's inhibitory activity, discovery compound A, B's is effective
Concentration IC50 is respectively 16.6 μM, 18.2 μM and shows anti-tumor activity.
The above content is just an example and description of the concept of the present invention, affiliated those skilled in the art
It makes various modifications or additions to the described embodiments or is substituted in a similar manner, without departing from invention
Design or beyond the scope defined by this claim, be within the scope of protection of the invention.
Claims (8)
1. a kind of preparation method of the antibacterial substance based on marine microorganism, it is characterised in that: specifically includes the following steps:
S1, bacterial strain isolate and purify
It takes the Indian Ocean 10g halmeic deposit sample in the test tube of sterilizing, 100mL antiseptic sea water is added, oscillation is uniformly mixed so as to obtain even
Slurry, this is 10-1Dilution takes turns doing gradient dilution in this way, obtains 10-2、10-3、10-4Dilution successively draws 100 μ
The seawater of each different dilutions of L is put in solid medium tablets, and liquid is uniformly coated on entire put down with sterile spreading rod
Plate surface is subsequently placed in 20 DEG C of incubators and cultivates 4-7 days；The hypha,hyphae with single bacterium colony is chosen from solid medium
Body, using plate streak, one ring hypha,hyphae of picking or spore cross under 5-6 in new planar surface, are then inverted in 20 DEG C
7 days are cultivated in incubator to get aspergillus fungi has been arrived；By purified aspergillus fungi strain inoculated in inclined-plane solid culture
In base conservation pipe, saved backup after aspergillus fungi bacterium colony grows up to；
S2, fermented and cultured
By aspergillus fungi bacterial strain on from conservation pipe inclined plane inoculating to fungi liquid culture medium plate, in 26 DEG C of cultures conduct in 7 days
Seed plate is inoculated into 30 with transfer needle picking fungus colony from liquid plate culture medium and contains 400ml fermented fungal liquid
In the 1000ml conical flask of culture medium, fermented and cultured 40 days under 26 DEG C, standing non-illuminated conditions, fermentation culture is obtained；
The extraction of S3, bacterial strain secondary metabolite
The obtained fermentation culture of step S2 is filtered with gauze, filtrate is extracted three times with isometric ethyl acetate, is fermented
Thick immersion liquid；Mycelium filter residue first uses 75% EtOH Sonicate to extract, after resulting extracting solution is concentrated into no ethyl alcohol, then in equal volume
Ethyl extraction three times, obtain the thick immersion liquid of mycelium, the thick immersion liquid that will ferment merges with the thick immersion liquid of mycelium to get bacterium has been arrived
Strain secondary metabolite crude extract；
The purifying of S4, bacterial strain secondary metabolite
Silica gel is added into bacterial strain secondary metabolite crude extract, crosses Rp-18 reverse phase silica gel column after mixing, uses petroleum
Ether: ethyl acetate gradient collects each eluent, is concentrated under reduced pressure, merges, isolated 2 antibacterial active compounds A
And B；
The structural formula of the antibacterial active compounds A and B are as follows:
2. a kind of preparation method of antibacterial substance based on marine microorganism according to claim 1, feature exist
In: the solid medium are as follows: wheat flour 17g, soluble starch 5g, glucose 3g, peptone 3g, agar 20g, chloramphenicol
0.1g, antiseptic sea water 1L, each component after mixing, use pH adjusting agent to adjust solid medium pH as 7.0-7.5.
3. a kind of preparation method of antibacterial substance based on marine microorganism according to claim 2, feature exist
In: the potassium dihydrogen phosphate aqueous solution that the pH adjusting agent is 20%.
4. a kind of preparation method of antibacterial substance based on marine microorganism according to claim 1, feature exist
In: in step S1, after being cultivated 7 days in incubator, if bacterium colony is impure, continues scribing line separation repeatedly, be up to obtaining single bacterium colony
Only.
5. a kind of preparation method of antibacterial substance based on marine microorganism according to claim 1, feature exist
In: in step S1, aspergillus fungi bacterium bacterium colony, which grows up to, is placed on 0-3 DEG C of refrigerator preservation.
6. a kind of preparation method of antibacterial substance based on marine microorganism according to claim 1, feature exist
In: the fluid nutrient medium are as follows: wheat flour 17g, yeast powder 2g, glucose 3g, peptone 3g, antiseptic sea water 1L, each component
After mixing, pH adjusting agent is used to adjust solid medium pH as 7.0-7.5.
7. a kind of preparation method of antibacterial substance based on marine microorganism according to claim 6, feature exist
In: the potassium dihydrogen phosphate aqueous solution that the pH adjusting agent is 10%.
8. a kind of preparation method of antibacterial substance based on marine microorganism according to claim 1, feature exist
In: the detailed process of gradient elution described in step S4 are as follows: use petroleum ether first: ethyl acetate=9:1 carries out elution 3-
4h, then with petroleum ether: ethyl acetate=1:1 obtains antibacterial active compounds A after collecting eluent concentration；Continue with petroleum
Ether: ethyl acetate=1:7.5 obtains antibacterial active compounds B after collecting eluent concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811139541.7A CN109112171A (en) | 2018-09-28 | 2018-09-28 | A kind of preparation method of the antibacterial substance based on marine microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811139541.7A CN109112171A (en) | 2018-09-28 | 2018-09-28 | A kind of preparation method of the antibacterial substance based on marine microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109112171A true CN109112171A (en) | 2019-01-01 |
Family
ID=64857051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811139541.7A Pending CN109112171A (en) | 2018-09-28 | 2018-09-28 | A kind of preparation method of the antibacterial substance based on marine microorganism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109112171A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109912604A (en) * | 2019-04-09 | 2019-06-21 | 中国科学院南海海洋研究所 | Quinazolinone alkaloid compound and preparation method thereof and preparing the application in liver X receptor agonists |
| CN113265337A (en) * | 2021-06-17 | 2021-08-17 | 华南农业大学 | Marine aspergillus versicolor and isolated culture method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60149520A (en) * | 1984-01-13 | 1985-08-07 | Ajinomoto Co Inc | Antitumor agent |
| CN101857841A (en) * | 2009-12-01 | 2010-10-13 | 大连交通大学 | Marine fungi aspergillus unguis strain, active extract thereof and preparation method and use of active extract thereof and active components thereof |
| CN103074234A (en) * | 2012-12-25 | 2013-05-01 | 浙江工业大学 | Marine fungus aspergillus sydowii and application thereof to preparation of anti-tumor medicines |
-
2018
- 2018-09-28 CN CN201811139541.7A patent/CN109112171A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60149520A (en) * | 1984-01-13 | 1985-08-07 | Ajinomoto Co Inc | Antitumor agent |
| CN101857841A (en) * | 2009-12-01 | 2010-10-13 | 大连交通大学 | Marine fungi aspergillus unguis strain, active extract thereof and preparation method and use of active extract thereof and active components thereof |
| CN103074234A (en) * | 2012-12-25 | 2013-05-01 | 浙江工业大学 | Marine fungus aspergillus sydowii and application thereof to preparation of anti-tumor medicines |
Non-Patent Citations (2)
| Title |
|---|
| MAHMOUD M. ELAASSER等: "Antioxidant, antimicrobial, antiviral and antitu mor activities of pyranone derivative obtained from Aspergillus candidus", 《J. MICROBIOL. BIOTECH. RES.》 * |
| 陈修文等: "深海来源曲霉16-02-1的代谢产物及其抗肿瘤抗真菌活性初步测评_", 《中国海洋药物》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109912604A (en) * | 2019-04-09 | 2019-06-21 | 中国科学院南海海洋研究所 | Quinazolinone alkaloid compound and preparation method thereof and preparing the application in liver X receptor agonists |
| CN113265337A (en) * | 2021-06-17 | 2021-08-17 | 华南农业大学 | Marine aspergillus versicolor and isolated culture method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11459593B2 (en) | Dendrobium officinale endophytic fungus strain and extracellular polysaccharide produced thereby, and extraction method and application of extracellular polysaccharide | |
| CN110468083B (en) | Separated streptomycete DL70 and biocontrol growth promotion application thereof | |
| CN104087511A (en) | Mucor racemosus strain and its application | |
| CN101294137B (en) | Arthrinium bacterial strain and uses thereof | |
| CN103898004A (en) | Pseudonocardia and method thereof for producing calcifediol by fermentation | |
| CN108660082A (en) | A kind of marine aspergillus source oxa- anthraquinone analog compound and preparation method thereof and the application in preparing antiseptic | |
| CN105886405A (en) | Dendrobium officinale Kimura et Migo endophytic fungus and applications thereof | |
| CN101993847B (en) | Bacterial cellulose strain | |
| CN103740606A (en) | Streptomyces phytohabitans, method for producing new antibiotics Novonestmycin from Streptomyces phytohabitans, and application of Novonestmycin | |
| WO2020181765A1 (en) | Geomyces mutant strain and application thereof | |
| CN109609491A (en) | It is a kind of it is efficient produce kasugarnycin streptomyces microaureus mutagenesis and screening technique | |
| CN104694401A (en) | Penicillium verrucosum LPPV001 and method for producing ergosterol peroxide by same | |
| CN109182147A (en) | A kind of mould and its method for producing fumidil | |
| CN109112171A (en) | A kind of preparation method of the antibacterial substance based on marine microorganism | |
| CN104774774B (en) | One plant of production pseurotin A glycyrrhiza endophytic fungi | |
| CN1928075B (en) | Strain capable of generating avermectin B component and utilization thereof | |
| CN109400444B (en) | Sesquiterpenoids for inhibiting plant pathogenic fungi and preparation method thereof | |
| CN107686817B (en) | Chrysanthemum bud endophytic fungus CYSK-4 and application of Ascomylactam compound produced by same | |
| CN113337432B (en) | Methylophilus for producing pyrroloquinoline quinone and application thereof | |
| CN109182216A (en) | One plant of marine streptomyces SCFJ-05 inhibited to succulent stem rot | |
| CN112143681B (en) | Bacillus belgii capable of producing feruloyl esterase and application thereof | |
| CN101508960B (en) | Rhizopus and uses thereof | |
| CN104059044A (en) | Xanthone derivative and preparation method and application of xanthone derivative | |
| CN113337403A (en) | Chaetomium globosum HJF 13 strain and application thereof | |
| CN102168020B (en) | Marine-source fungus and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190101 |
|
| RJ01 | Rejection of invention patent application after publication |