CN109022283A - A kind of method of stable liquid photosynthetic bacteria active bacteria formulation - Google Patents
A kind of method of stable liquid photosynthetic bacteria active bacteria formulation Download PDFInfo
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- CN109022283A CN109022283A CN201810925435.5A CN201810925435A CN109022283A CN 109022283 A CN109022283 A CN 109022283A CN 201810925435 A CN201810925435 A CN 201810925435A CN 109022283 A CN109022283 A CN 109022283A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
Abstract
The invention discloses a kind of methods of stable liquid photosynthetic bacteria active bacteria formulation, in photosynthetic bacteria suspension, the macromolecule organic medium of noresidue that photosynthetic bacteria is difficult to be utilized, nontoxic is added, photosynthetic bacteria liquid active bacteria formulation is made, macromolecule organic concentration of medium range is 3.0~6.0g/L.Said preparation can at least store 12 months in 4 DEG C~30 DEG C dark surrounds, liquid preparation appearance color has no significant change, preparation character uniformity, no longer there is apparent liquid preparation thallus sedimentation lamination, and bioactivity still with higher, removal nitrite nitrogen ability maintain 80% or more.The active bacteria formulation by storage can be miscible with water, is easily dispersed in water, has no obvious thallus agglomerate.The preparation method of preparation is described in detail in the present invention, also develops the media such as xanthan gum in the new application in photosynthetic bacteria product producer face.This method is easy to operate, low in cost, is easy to scale operation.
Description
Technical field
The present invention relates to a kind of technical field of microbiology, mention more particularly to one kind in photosynthetic bacteria preparation production process
The method of high photosynthetic bacteria preparation storage stability.
Background technique
Currently, ((Anoxygenic phototrophic bacteria, APB) is that one kind is being detested to Anoxygenic photosynthetic bacteria
Under the conditions of oxygen, oxygen photosynthesis prokaryotic micro-organisms is not put as electron donor using a variety of small organic molecules or inorganic matter
General name, it is traditionally known as " photosynthetic bacteria (PSB) ".Existing research shows that photosynthetic bacteria monoid has more than 80 categories more than 200
Kind, in the natural environments such as widely distributed soil, rivers and lakes, ocean, there is weight in nature substance circulation and energy circulation
It acts on；Its metabolic way is versatile and flexible, can be carried out light autotrophy, light heterotrophism and Heterotrophic growth, mutually fits with its living environment
It answers.Photosynthetic bacteria is also a kind of important living resources, currently, photosynthetic bacteria is in microorganism resource, production equipment, preparation and product
And application etc. achieves impressive progress, photosynthetic bacteria preparation has been widely used in various fields, such as: in aquaculture
It is middle to be used as bait, additive of bait and water quality cleansing agent, agriculturally it is being used as microbial manure or plant growth regulator, is raiseeing
Animal husbandry is used as additive for microbe feedstuff.In addition, photosynthetic bacteria pollution environment biological prosthetic and administer, medicines and health protection and
Also there is in-depth study in the fields such as the energy.
Currently, photosynthetic bacteria preparation is many kinds of in the market, overview its preparation type that gets up mainly includes liquid viable bacteria system
Agent and solid-state active bacteria formulation two major classes, solid formulation storage stability is good, but it is slow to play effect.Liquid preparation plays effect
Fastly, but during storage liquid preparation is unstable.For photosynthetic bacteria preparation because strain, culture medium and condition of culture etc. are different, bacterium is outstanding
Aubergine, bronzing, kermesinus, yellow green etc., when shaking up, bacteria suspension attractive color is presented in liquid.But most liquid are photosynthetic
Bacteria preparation is layered within 1~3 week in standing storage or even thallus aggregation extent is different, multilayer occurs.Especially stand storage
2~March or longer time, thallus even fall to container bottom, the clarification that the bacteria suspension of upper vessel portion almost becomes, preparation character
Unevenly.Furthermore the degree due to transporting, shaking in handling process is different, the same a collection of product in Different Package bottle, bacteria suspension
Character is irregular, although shaking up using product effect is not influenced, product appearance is severely impacted, and cannot be showed photosynthetic
The tempting color of bacterium product.Especially one plant of photosynthetic bacteria preparation for being exclusively used in sea-farming of inventor's exploitation ---
Ocean chomophoric bacterium YL28 preparation, deposit number CGMCC7.313, the bacterium can grow by only nitrogen source of nitrite nitrogen, efficiently removal sea
The harmful substances such as ammonia nitrogen and nitrite nitrogen in water breeding water body.But the thallus individual cells are larger and easy aggregation, have very strong
The adherent characteristic of the light that becomes in addition total Fungal biodiversity in culture vessel 70% be attached to culture vessel inner surface.The strain
Culture medium salinity is high (3.0%NaCl), the bacteria suspension of culture, and thallus is easy to sedimentation layering when standing, and big aggregation sedimentation is fast,
Small aggregation sedimentation is slow, and preparation is very unstable, is obviously layered within static storage 1~3 day, the thallus in about 20 days preparations is several
Container bottom is fallen to, the appearance of product is seriously affected.It investigates by literature search, there is no the stable liquid of good method is photosynthetic thin
Bacteria preparation.Opaque container filling photosynthetic bacteria liquid preparation is mostly used at present, or covers container outer surface, table with label paper
It can't see the sedimentation layering of preparation in sight, there is no fundamentally solve the problems, such as this.
Summary of the invention
It is stored for a long time, in view of this, being conducive to photosynthetic bacteria liquid viable bacteria microbial inoculum the purpose of the present invention is to provide one kind
The method of even stabilization liquid photosynthetic bacteria active bacteria formulation that is consistent, that apparent sedimentation lamination no longer occur.
In order to achieve the above objectives, the technical scheme is that
It is difficult that photosynthetic bacteria is added in photosynthetic bacteria bacteria suspension in a kind of method of stable liquid photosynthetic bacteria active bacteria formulation
With the macromolecule organic medium of noresidue utilize, nontoxic, photosynthetic bacteria liquid active bacteria formulation is made, in photosynthetic bacteria
In liquid active bacteria formulation, macromolecule organic concentration of medium is within the scope of 3.0~6.0g/L.
Further, macromolecule organic medium includes one of xanthan gum, carragheen, Weilan gum and gelatin or more
Kind, xanthan gum, carragheen, Weilan gum or gelatin concentration respectively within the scope of 3.0~6.0g/L.
Further, macromolecule organic medium solution is added in photosynthetic bacteria suspension by sterile working method.
Further, during sterile working, macromolecule organic medium is made an addition in water, being configured to macromolecule has
Machine object medium solution, stirring and dissolving in process for preparation stops stirring, and uses high-temp steam sterilizing after dissolution, cooling after sterilizing
42 DEG C are not higher than to temperature, sterile macromolecule organic medium solution is obtained, under agitation, by photosynthetic bacteria viable bacteria suspension
Stream is added in the container containing sterile macromolecule organic medium solution, is stirred evenly, and filling can be stored.
Further, macromolecule organic medium solution is in process for preparation, xanthan gum and Weilan gum in be heated to 50~
60 DEG C are dissolved, and carragheen and gelatin are heated to 80~90 DEG C and are dissolved.
Further, mass concentration 3.0%NaCl is contained up in macromolecule organic medium solution.
After adopting the above technical scheme, a kind of method of stable liquid photosynthetic bacteria active bacteria formulation of the present invention, has following
The utility model has the advantages that
One, the present invention provides a kind of methods of stable liquid photosynthetic bacteria preparation to add in photosynthetic bacteria viable bacteria suspension
The macromolecule organic medium for adding photosynthetic bacteria to be difficult to be utilized, changes the colloidal nature of bacteria suspension, and improves bacteria suspension
Viscosity, is adsorbed on the thallus in photosynthetic bacteria suspension on colloidal medium, effectively prevents thallus aggregation sedimentation, improves light
Close bacterium active bacteria formulation storage stability.
Photosynthetic bacteria viable bacteria liquid preparation can be made at 4 DEG C~30 DEG C by photosynthetic bacteria active bacteria formulation prepared by this method
Dark surrounds at least stores 12 months, preparation stabilization, uniformity, not shown apparent sedimentation lamination, attractive color, real
Photosynthetic bacteria liquid active bacteria formulation is showed to store steadily in the long term, has efficiently solved existing photosynthetic bacteria liquid preparation and stored
In journey the problem of thallus sedimentation layering.Photosynthetic bacteria preparation using this method preparation is in liquid condition, and energy and water are with any ratio
Example mixing, is easily dispersed in water, is easy to use.These macromolecule organic media tool has been widely used at present, safety
It is nontoxic.
Two, nontoxic, the harmless macromolecule organic without hazard residue that the present invention has selected photosynthetic bacteria to be difficult to be utilized is situated between
Matter changes the colloidal nature of bacteria suspension, improves the viscosity of bacteria suspension, and the thallus in photosynthetic bacteria suspension is made to be adsorbed on colloid
On medium, thallus aggregation sedimentation is effectively prevented.Photosynthetic bacteria during storage do not breed by these agent growths of energy metabolism,
Thus stable storing.These substances itself are nontoxic, it is harmless, without hazard residue, these substances such as xanthan gum, Weilan gum and carragheen
Deng mainly polysaccharose substance is used as fertilizer or water quality cleansing agent, the effect of irritating certain micro-organisms growth, to light
Closing bacteria preparation has synergistic effect.
Three, xanthan gum, Weilan gum, gelatin and carragheen etc. can be used for food additives, pharmaceutical preparation, cosmetics, oral cavity
Protect product, life detergent etc..Industrial grade xanthan gum be widely used in oil drilling, pesticide, feed, ceramics, printing and dyeing, ore dressing,
Paint and coating etc..The present invention develops these macromolecule organics, if xanthan gum, Weilan gum, gelatin and carragheen are photosynthetic
New application on bacterium production.This method is simple to operate, low in cost, and production scale is changeable, is easy to scale
Change operation.
Specific embodiment
In order to further explain the technical solution of the present invention, being explained in detail below by specific embodiment the present invention
It states.
Embodiment 1
A kind of method of stable liquid photosynthetic bacteria preparation of the present invention, is added in 10L specification transparent glass reactor
2.0L distilled water, in room temperature environment, under stirring condition, after 75.0g NaCl dissolution is added, then slowly (a small amount of multiple) is added
50.0g xanthan gum forms xanthan gum mixed liquor, and the amount added every time is advisable with can finally be completely dissolved, and xanthan gum mixed liquor is held
It is continuous to be heated with stirring to 50 DEG C, it is stirred for being completely dissolved to xanthan gum for 10 minutes, forms xanthan gum solution, xanthan gum solution carries out high
121 DEG C of steam of pressure sterilize 30 minutes, and temperature is naturally cooled to after sterilizing not higher than 42 DEG C, obtains sterile macromolecule organic medium
Solution supplements sterile water to 2.50L scale in sterile macromolecule organic medium solution, stirs evenly, xanthan in reactor
Glue mass-volume concentration (hereinafter referred concentration) is 20.0g/L, i.e., contains 20g xanthan gum in every 1L solution.NaCl content is matter
It measures concentration 3.0% (w/v).In the present invention, NaCl content can demand according to different photosynthetic bacterias to osmotic pressure or salinity carry out
Adjustment, is generally not more than 3.0%.
By 7.5L purple sulfur bacteria ocean chomophoric bacterium (Marichromatium gracile) YL28 (deposit number
CGMCC7.313 bacteria suspension (biomass OD)660It is added in above-mentioned reactor, stirs 10 minutes for 4.53) stream, be uniformly mixed,
Then it is dispensed into cylindrical transparent plastic bottle, every bottle of filling 580mL, liquid level 200mm, seals, obtain several bottles of YL28
Bacteria preparation.Xanthan gum concentration is 5.0g/L in the YL28 bacteria preparation.Final Fungal biodiversity (the OD of the YL28 bacteria preparation660) be
3.40.In the present invention, with the light absorption value of spectrophotometric determination bacteria liquid sample, with the absorbance value (OD at 660nm660) represent
The biomass of each bacteria liquid sample, light absorption value is bigger, and biomass is more in bacteria liquid sample.According to the demand of YL28 bacteria preparation, weight
Multiple above-mentioned steps carry out the production of YL28 bacteria preparation.Above-mentioned ocean chomophoric bacterium YL28 is separated by this room and is identified, is detailed in " one plant of document
Mangrove ocean purple sulfur bacteria separation identification and characteristic of the height containing rhodopin, microorganism journal, 2011,51 (10): 1318-
1325”.
It takes 15 bottles of YL28 bacteria preparations to be placed in dark carton, storage is stood in 30 DEG C of culturing room.Separately take 15 bottles of YL28 bacterium
Preparation is placed in storage in 4 DEG C of refrigerators (dark storage), for observing the storage situation of preparation.With the YL28 bacterium system stored at 30 DEG C
Agent is room temperature group, using the YL28 bacteria preparation stored at 4 DEG C as low temperature group.Room temperature group and low temperature group were respectively at the 0th month, March,
Observe in each bottle YL28 bacteria preparation the color of thallus suspension and sedimentation situation in June and December, while in each observation stage from normal
Warm group or low temperature group take 3 bottles for measuring thallus denitrification activity respectively, indicate microbial activity with thallus removing nitrite nitrogen activity.It takes
YL28 bacteria preparation is shaken up before sample, is sampled, is inoculated in the measurement system containing about 20mg/L nitrite nitrogen (wherein by 3% (v/v) amount
Nitrite nitrogen be subject to measured value), 3% (v/v) indicates that the volume of inoculation thallus suspension accounts for the 3% of measurement system volume.Inoculation
There is the measurement system of YL28 thallus in 30 DEG C, 3000lx illumination cultivation 4 days, nitrite nitrogen content in measurement system, meter is measured by sampling
Nitrous nitrogen removal efficiency is calculated, the measurement system setting for being each inoculated with YL28 thallus is repeated 3 times measurement nitrous nitrogen content, finally with flat
Mean value indicates.In the present invention, measuring method and nitrate nitrogen the removal rate calculating of nitrous nitrogen content belong to common knowledge.
It was observed by 12 months, the YL28 active bacteria formulation appearance color of 4 DEG C and 30 DEG C storages is in peony always, has no bright
Aobvious variation, other than in addition to bottle top, about only 5mm liquid level is clarified, whole bottle other parts bacteria suspension uniform color is consistent, has no that thallus is heavy
The phenomenon that drop layering.YL28 bacteria preparation is poured into water jog, is easily dispersed in water, is occurred without apparent agglomerate.4 DEG C of storages
When depositing 0 month, 3 months, 6 months and 12 months, the nitrous nitrogen removal efficiency of low temperature group mutually should be 100% respectively, 97.6%,
When 93.5% and 87.2%, 30 DEG C storage 0 month, 3 months, 6 months and 12 months, the nitrous nitrogen removal efficiency of room temperature group distinguishes phase
It should be 100%, 95.8%, 90.2% and 84.3%.It can be seen that under the conditions of 4 DEG C~30 DEG C of temperature and 0-12 months guarantors
It deposits in the time, the denitrification activity of thallus is held in 80% or more.Therefore, YL28 bacteria preparation at least can be reserved for 12 months.In this way,
Consider from use cost, YL28 bacterium (i.e. photosynthetic bacteria) preparation is preferably saved in cool place, room temperature, ventilation dark, can be saved
Save the cost of refrigeration and cold chain transportation.
Embodiment 2
A kind of method of stable liquid photosynthetic bacteria preparation of the present invention, in about 750mL distilled water beaker is housed, in room temperature
In environment, under magnetic agitation, 24.00g NaCl, 9.60g xanthan gum, 2.40g Weilan gum and 2.40g gelatin are sequentially added, is formed
Mixed liquor continues to be stirred liquid and heat, and is arranged 90.0 DEG C of heating temperature, yellow when mixed liquor is warming up to 50~60 DEG C
Virgin rubber and Weilan gum are dissolved, and when mixed liquor is warming up to 80~90.0 DEG C, gelatin is dissolved, when mixed liquor is warming up to 90
DEG C when persistently stir 10 minutes again, dissolution completely, forms multimedium solution, and in the multimedium solution plus distilled water is to 800mL,
The sum of concentration of above-mentioned three kinds of media is 18.0g/L, i.e., contains xanthan gum, Weilan gum and the total 18g of gelatin in every 1L solution, obtain
Macromolecule organic medium solution.
800mL macromolecule organic medium solution is transferred in the vial of 3.0L specification, with 121 DEG C of high-pressure sterilizing pot
Sterilizing 30 minutes, is cooled to about 42 DEG C, in aseptic superclean bench, by magnetic agitation, is slowly added into purple sulfur bacteria sea
Bacteria suspension (the bacteria suspension biomass OD of foreign chomophoric bacterium (Marichromatium gracile) YL28660For 4.53) 1600mL, stir
It mixes uniformly, (highly in the grinding port plug glass colorimetric cylinder of 220mm), the filling about 70mL of every pipe, liquid level is high for packing to 50mL specification
Degree is 200mm, is sealed again with sealed membrane after jumping a queue, obtains several pipe YL28 bacteria preparations.In the YL28 bacteria preparation, xanthan gum, Welland
Glue and gelatin concentration are followed successively by 4.0g/L, 1.0g/L and 1.0g/L.The YL28 bacteria preparation Fungal biodiversity (OD660) it is 3.02.
It takes 15 pipe YL28 bacteria preparations to be placed in black carton, storage is stood in culturing room.Separately take 15 pipe YL28 bacteria preparations
It is placed in storage in 4 DEG C of refrigerators (dark storage), for observing the storage situation of preparation.It is with the YL28 bacteria preparation cultivated at 30 DEG C
Room temperature group, using the YL28 bacteria preparation stored at 4 DEG C as low temperature group.Room temperature group and low temperature group were respectively at the 0th month, March, June
With observe in each pipe YL28 bacteria preparation the color of thallus suspension and sedimentation situation December.Simultaneously in each observation stage from room temperature group
Take 3 pipes for measuring thallus denitrification activity respectively with low temperature group.It is de- according to 1 correlation method of embodiment measurement thallus in the present embodiment
Nitrogen activity indicates microbial activity with thallus removing nitrite nitrogen activity.
It was observed by 12 months, the YL28 active bacteria formulation appearance color of 4 DEG C and 30 DEG C storages is in peony always, has no bright
Aobvious variation, homogeneous tube bacteria suspension uniform color is consistent, does not observe that pipe top liquid level clarifies phenomenon.YL28 bacteria preparation is poured into water
Jog is easily dispersed in water, is occurred without apparent agglomerate.It is low at 4 DEG C storage 0 month, 3 months, 6 months and 12 months
The nitrous nitrogen removal efficiency of temperature group mutually should be 99.8%, 96.1%, 90.4% and 86.3% respectively.30 DEG C store 0 month, 3 months,
At 6 months and 12 months, the nitrous nitrogen removal efficiency removal rate of room temperature group mutually should be 100% respectively, 94.7%, 88.1% and
83.2%.It follows that the denitrification activity of thallus is equal under the conditions of 4 DEG C~30 DEG C of temperature and in 0-12 months holding times
80% or more, and appearance luster uniformity are kept, preparation has no obvious thallus aggregation agglomerate.Therefore, YL28 bacteria preparation is extremely
It can be reserved for 12 months less.
Embodiment 3:
A kind of method of stable liquid photosynthetic bacteria preparation of the present invention, in about 350mL distilled water beaker is housed, in room temperature
In environment, under magnetic agitation, 12.00g NaCl and 4.0g carragheen is sequentially added, mixed liquor is formed, continues to be stirred liquid simultaneously
Heating, be arranged 80.0 DEG C of heating temperature, be warming up to after 80.0 DEG C and persistently stir 10 minutes, then plus distilled water to 400mL scale, obtain
To the carrageenan solutions of 10.0g/L, wherein NaCl content is 3.0% (w/v).
The carrageenan solutions 360mL for taking the 10.0g/L of preparation is transferred in the blue lid bottle of 2L specification, high-pressure sterilizing pot 121
DEG C sterilizing 30 minutes, be cooled to about 42 DEG C, in aseptic superclean bench, by magnetic agitation, be slowly added into purple sulfur bacteria
Bacteria suspension (the bacteria suspension biomass OD of ocean chomophoric bacterium (Marichromatium gracile) YL28660For 4.53) 840mL,
It stirs evenly, and packing to 50mL specification (highly in the grinding port plug glass colorimetric cylinder of 220mm), the filling about 70mL of every pipe, liquid level
Height is 200mm, is sealed after jumping a queue with sealed membrane.Obtain several pipe YL28 bacteria preparations.In the YL28 bacteria preparation, carrageenan concentrations are
3.0g/L, the YL28 bacteria preparation Fungal biodiversity (OD660) it is 3.17.
It takes 15 pipe YL28 bacteria preparations to be placed in black carton, storage is stood in 30 DEG C of culturing room.Separately take 15 pipe YL28 bacterium
Preparation is placed in storage in 4 DEG C of refrigerators (dark storage), for observing the storage situation of preparation.With the YL28 bacterium system stored at 30 DEG C
Agent is room temperature group, using the YL28 bacteria preparation stored at 4 DEG C as low temperature group.Room temperature group and low temperature group were respectively at 0 month, 3 months, 6
Observe within a month and 12 months the color of thallus suspension and sedimentation situation in each pipe YL28 bacteria preparation.Simultaneously in each observation stage from normal
Warm group or low temperature group take 3 pipes for measuring thallus denitrification activity respectively.Bacterium is measured according to 1 correlation method of embodiment in the present embodiment
Body denitrification activity indicates microbial activity with thallus removing nitrite nitrogen activity.
It was observed by 12 months, the YL28 active bacteria formulation appearance color of 4 DEG C and 30 DEG C storages is in peony always, has no bright
Aobvious variation, other than the clarification of pipe top about 15mm liquid level, homogeneous tube other parts bacteria suspension uniform color is consistent, has no that thallus settles
The phenomenon that layering.YL28 bacteria preparation is poured into water jog, is easily dispersed in water, is occurred without apparent agglomerate.30 DEG C of storages
When depositing 0 month, 3 months, 6 months and 12 months, nitrous nitrogen removal efficiency mutually should be 100%, 95.4%, 90.2% and respectively
85.1%.It follows that saving 12 months at 30 DEG C, the denitrification activity of thallus still keeps 80% or more, and appearance luster is equal
Even consistent, preparation has no obvious thallus aggregation agglomerate.The denitrification activity of reference experiment example 1-2 room temperature group and low temperature group measurement knot
Fruit, the present embodiment 4 DEG C of conditions of storage of default are more advantageous, therefore the present embodiment omits 4 DEG C of denitrification activity measurement.
Embodiment 4:
A kind of method of stable liquid photosynthetic bacteria preparation of the present invention, weighs 4.80g xanthan gum, is stirred at room temperature and is dissolved in
In 400mL distilled water, concentration 12.0g/L is cooled to room temperature through high-temp steam sterilizing, is added on superclean bench
800mL Rhodopseudomonas palustris CQV97 (deposit number MCCC1I00117) bacteria suspension, Fungal biodiversity (OD660) it is 4.52,
It stirs evenly, is sub-packed in grinding port plug glass colorimetric cylinder, every pipe filling about 70mL, liquid level 200mm, with sealing after jumping a queue
Film sealing, obtains several pipe CQV97 bacteria preparations, and in the CQV97 bacteria preparation, xanthan gum concentration is 4.0g/L, in the CQV97 bacteria preparation
Fungal biodiversity (OD660) it is 3.01.Other Rhodopseudomonas palustris bacterial strains (such as CGMCC 1.2180) also can be used in the present embodiment
Bacteria suspension tested.
15 pipe CQV97 bacteria preparations are taken to be placed in black carton, in standing storage in 30 DEG C of culturing room, for observing preparation
Store situation.The color and sedimentation feelings of each pipe CQV97 bacterium thallus suspension are observed respectively at 0 month, 3 months, 6 months and 12 months
Condition.Take 3 pipes for measuring thallus denitrification activity respectively in each observation stage simultaneously.According to 1 respective party of embodiment in the present embodiment
Method measures thallus denitrification activity, indicates microbial activity with thallus removing nitrite nitrogen activity.
It was observed by 12 months, the CQV97 active bacteria formulation appearance color of storage takes on a red color always, has no significant change, whole
The phenomenon that pipe bacteria suspension uniform color is consistent, has no thallus sedimentation layering.CQV97 bacteria preparation is poured into water jog, in water
It is easily dispersed, occurs without apparent agglomerate.At 30 DEG C storage 0 month, 3 months, 6 months and 12 months, nitrous nitrogen removal efficiency
It mutually should be 100%, 93.4%, 87.3% and 83.6% respectively.It follows that saving 12 months at 30 DEG C, the denitrogenation of thallus is living
Property still keep 80% or more, and appearance luster uniformity, preparation has no obvious thallus aggregation agglomerate.
It should be noted that increase cost of material of the invention is analyzed as follows: carried out by taking xanthan gum as an example increase raw material at
This analysis, the maximum usage amount of xanthan gum of the present invention are 6.0g/L, and cube rice product dosage is 6kg.Xanthan gum retail price is
10.0 yuan/kg, it is 60.0 yuan that product maximum per ton, which increases cost of material, and every liter of product maximum increases cost of material 6 and divides money, is increased
Cost of material is lower.For fermentation plant, it is equipped with material-compound tank, sterilization tank, method of the invention can not increase equipment
It is produced as a trial under conditions of cost.
Technical advantage and feature of the invention
One, the present invention provides a kind of raising photosynthetic bacteria viable bacteria liquid preparation storage stability methods.This method can
Store photosynthetic bacteria viable bacteria liquid preparation at least 12 months in 4 DEG C~30 DEG C dark surrounds, preparation stabilization, uniformity, not
Apparent sedimentation lamination is presented, attractive color realizes photosynthetic bacteria liquid active bacteria formulation and stores steadily in the long term, effectively
Ground solves the problems, such as thallus sedimentation layering during existing photosynthetic bacteria liquid preparation storage.
Two, nontoxic, the harmless macromolecule organic without hazard residue that the present invention has selected photosynthetic bacteria to be difficult to be utilized is situated between
Matter changes the colloidal nature of bacteria suspension, improves the viscosity of bacteria suspension, and the thallus in photosynthetic bacteria suspension is made to be adsorbed on colloid
On medium, thallus aggregation sedimentation is effectively prevented.Photosynthetic bacteria during storage do not breed by these agent growths of energy metabolism,
Thus stable storing.These substances itself are nontoxic, it is harmless, without hazard residue, these substances such as xanthan gum, Weilan gum and carragheen
Deng mainly polysaccharide material is used as fertilizer or water quality cleansing agent, the effect of irritating certain micro-organisms growth, to photosynthetic
Bacteria preparation has synergistic effect.
Three, the present invention develops these macromolecule organics, if xanthan gum, Weilan gum, gelatin and carragheen are photosynthetic thin
New application on bacterium production.This method is simple to operate, low in cost, and production scale is changeable, is easy to scale
Operation.
Above-described embodiment and non-limiting method of the invention, any person of an ordinary skill in the technical field do it
Appropriate changes or modifications, all should be regarded as not departing from patent category of the invention.
Claims (6)
1. a kind of method of stable liquid photosynthetic bacteria active bacteria formulation, it is characterised in that: in photosynthetic bacteria bacteria suspension, light is added
The macromolecule organic medium for closing noresidue that bacterium is difficult to be utilized, nontoxic, is made photosynthetic bacteria liquid active bacteria formulation,
In photosynthetic bacteria liquid active bacteria formulation, macromolecule organic concentration of medium is within the scope of 3.0~6.0g/L.
2. a kind of method of stable liquid photosynthetic bacteria active bacteria formulation as described in claim 1, it is characterised in that: macromolecule has
Machine object medium includes one of xanthan gum, carragheen, Weilan gum and gelatin or a variety of, xanthan gum, carragheen, Weilan gum or bright
The concentration of glue is respectively within the scope of 3.0~6.0g/L.
3. a kind of method of stable liquid photosynthetic bacteria active bacteria formulation as claimed in claim 2, it is characterised in that: by sterile
Macromolecule organic medium solution is added in photosynthetic bacteria suspension by operating method.
4. a kind of method of stable liquid photosynthetic bacteria active bacteria formulation as claimed in claim 3, it is characterised in that: in sterile behaviour
During work, macromolecule organic medium is made an addition in water, macromolecule organic medium solution is configured to, is stirred in process for preparation
Dissolution is mixed, stops stirring after dissolution, and use high-temp steam sterilizing, temperature is cooled to after sterilizing not higher than 42 DEG C, is obtained sterile
Photosynthetic bacteria viable bacteria suspension stream is added to organic containing sterile macromolecule by macromolecule organic medium solution under agitation
It in the container of object medium solution, stirs evenly, filling can store.
5. a kind of method of stable liquid photosynthetic bacteria active bacteria formulation as claimed in claim 3, it is characterised in that: macromolecule has
Machine object medium solution is in process for preparation, and xanthan gum and Weilan gum are dissolved in being heated to 50~60 DEG C, carragheen and gelatin
80~90 DEG C are heated to be dissolved.
6. a kind of method of stable liquid photosynthetic bacteria active bacteria formulation as claimed in claim 5, it is characterised in that: macromolecule has
Mass concentration 3.0%NaCl is contained up in machine object medium solution.
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