CN109012770B - Multilayer paper chip structure, manufacturing apparatus and method, and fluid interlayer flow method - Google Patents
Multilayer paper chip structure, manufacturing apparatus and method, and fluid interlayer flow method Download PDFInfo
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
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- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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Abstract
The invention discloses a multilayer paper chip structure, which comprises at least two layers of paper substrates, wherein at least one fluid space is formed between the layers of the paper substrates and a resin layer; the fluid space comprises a sample introduction area, the sample introduction area is connected with a fluid channel, and the fluid channel is connected with at least one detection area; the sample injection areas of the paper substrate are on the same straight line perpendicular to the paper substrate. The invention also provides a manufacturing device and a manufacturing method of the multilayer paper chip and a method for controlling the flow between the fluid layers. The invention has the advantages that the multilayer structure of the multilayer paper chip can realize complex multistep biological analysis on paper; the fluid channel is not filled with cellulose, so that interlayer isolation is realized, and interlayer flow of fluid is realized only by applying an electric field or airflow action to the outside, so that the detection process has controllability; the manufacturing method realizes process automation and realizes automatic adhesion and alignment among different layers of paper; the fluid laminar flow method uses electric fields or air flows to control, making the control process programmable and automated.
Description
Technical Field
The present invention relates to the field of paper core structures, methods of making and using, and more particularly to multi-layer paper core structures, apparatus and methods of making and methods of inter-layer flow of fluids.
Background
The paper chip microfluid device is firstly proposed in 2007 by the Whitesids research group of the Harvard university in USA, specifically, paper is used for replacing traditional microfluidic substrates such as glass, silicon and high polymer, a flow path is processed on the surface of the paper, liquid transportation and transfer are achieved by means of the inherent capillary force of the paper, and the paper chip microfluid device has the advantages of being light, low in cost, easy to obtain, good in biocompatibility and the like. According to the flowing direction, the paper chip microfluid can be divided into a two-dimensional paper chip and a three-dimensional paper chip, and the preparation method of the two-dimensional paper chip comprises wax printing, ink-jet printing, photoetching, plasma etching and the like. Three-dimensional paper chips provide significant improvements in detection capacity, complexity and functionality of the device, as compared to two-dimensional paper chips. Santhiago et al propose the use of double-sided adhesive to bond folded paper to make a three-dimensional paper chip electrochemical detection device, which, although relatively simple, still requires accurate manual alignment during the manufacturing process. Whitesides et al apply multiple layers of paper printed with different patterns with double sided tape, perforated at designated locations on the double sided tape and then filled with cellulose to allow the solution to penetrate from the top layer to the next; the manufacturing process is complex and has high precision requirement, and the filled cellulose has adsorption effect on molecules. Later, Crooks et al proposed a paper folding method, and the paper folding method involves the mirror image problem of paper folding, and folding precision requires highly, still needs auxiliary device so that upper and lower adjacent two-layer in close contact with simultaneously. Lewis et al propose to use wax printing technology in combination with glue spraying to prepare multi-layer paper chips, which, although highly efficient, has high operating requirements for laboratory personnel and is very expensive in wax-spraying printers. At present, no research report is available on a method for integrally and automatically preparing a multilayer paper chip microfluid device.
The control methods for liquid flow on paper chips are mainly classified into three categories. The most direct method is to provide flow paths with different lengths or widths, and the flow time is prolonged along with the increase of the lengths or widths of the paths. Second, changing the wettability of the paper surface with chemical agents is the second type of method for controlling the flow of liquids, which includes sugars, paraffins, surfactants, alkyl ketene dimers, gels, and the like; hydrophilic channels are first engraved in the paper by conventional methods for making single-layer paper microfluidic devices, and then the channels are treated with chemicals, the flow rate of the liquid in the channels slowing down with the increase in the concentration of the chemicals. This method adds a paper chip preparation step and paper chips treated with chemical reagents may interfere with the detection of certain compounds. The third method is to control the flow of liquid by mechanical means, such as an electromagnet controlling the opening and closing of a flow path, a triboelectric effect accelerating the flow of liquid, a soluble bridge transferring a limited amount of liquid, a water-absorbent sponge shunting the liquid, a movable valve connecting the flow path, etc.; the above method is mainly directed to the control of liquid flow on single-ply paper, and the equipment preparation process and the liquid handling process are relatively complicated. Therefore, at present, the on-off control of the liquid can be realized on the single-layer paper chip, the on-off of the liquid is only controlled in a delayed manner by the multi-layer paper chip, but the on-off control of the liquid in the multi-layer paper chip cannot be realized.
Disclosure of Invention
In order to solve the problems, the invention provides a structure of a multilayer paper chip and provides a method for simply, integrally and automatically preparing the multilayer paper chip; the equipment is simple in structure, the multi-layer paper is automatically bonded and aligned in the printing process, the use of adhesive is avoided, and human errors are reduced. Meanwhile, a method for controlling the microfluid to flow between the paper chip layers is also provided, and the method is particularly suitable for complex multistep bioanalysis on paper, such as enzyme-linked immunosorbent assay, signal amplification immunoreaction, nucleic acid detection and the like; the control program of the electric field or the airflow is programmed by utilizing Arduino software, and the liquid on the upper layer flows downwards in sequence according to set time, so that the multistep complex reaction can be automatically carried out, the artificial participation is reduced, and the accuracy and the repeatability of the multistep reaction can be improved.
In order to achieve the purpose, the invention adopts the following technical scheme: the multilayer paper chip structure comprises at least two layers of paper substrates, wherein at least one fluid space is formed between the layers of the paper substrates and the resin layer; the fluid space comprises a sample introduction area, the sample introduction area is connected with a fluid channel, and the fluid channel is connected with at least one detection area; the sample injection areas of the same fluid space are on the same line perpendicular to the paper substrate.
Further, the multilayer paper chip structure still includes, and the top paper substrate surface still has the stratum basale, and the stratum basale is printed by the resin and is formed.
Further, the material of the paper base is filter paper.
Further, the multilayer paper chip manufacturing equipment comprises a light source, a light transmission device, a reaction container and a platform; wherein the light transmission device is used for realizing and changing the shape of the light source transmission area; the light transmission device is arranged above the light source; a reaction vessel containing a resin liquid; the light transmission device is arranged on the bottom surface of the reaction vessel, and the light source can transmit into the reaction vessel through the light transmission device; the platform can vertically move up and down and is used for placing a semi-finished product or a finished product of the paper chip in the process of printing the paper chip; when the platform is at the lowest position, the distance between the platform and the bottom surface of the reaction vessel is used as the thickness of the resin.
Further, the light source is an LED lamp.
Further, the light transmission device is an LCD panel for receiving and displaying the shape of the computer designed transmission area through which the light source can enter the reaction vessel.
Further, the method for manufacturing the multilayer paper chip comprises the following steps:
s1, downloading the designed shape of the transmission area from the computer by the light transmission device, and descending the platform to the distance between the platform and the bottom surface of the reaction container to be the set thickness of the substrate layer; turning on a light source, exposing according to set time, and printing a blank basal layer; after the blank substrate layer is printed, the light source is turned off, the platform rises, and the substrate layer is adhered to the lower surface of the platform;
s2, placing a paper substrate in the reaction container, descending the platform to a set position, turning on the light source, exposing according to set time, and printing a first resin layer; after the resin layer is printed, the light source is turned off, the platform rises, and the paper chip semi-finished product is adhered to the lower surface of the platform;
s3, placing a paper substrate in the reaction container, downloading the designed shape of the transmission area from the computer by the light transmission device, descending the platform to a set position, turning on the light source, exposing according to a set time, and printing the resin layer; after the resin layer is printed, the light source is turned off, the platform rises, and the paper chip semi-finished product is still adhered to the lower surface of the platform;
s4, repeat S3 until printing of all resin layers is completed.
Further, the multilayer paper chip fluid interlayer flowing method comprises the following steps: and (3) dripping a sample to be detected into the outermost sample injection region of the paper chip, and applying an electric field to two sides of the sample injection region of the paper chip or applying air flow to one side of the sample injection region of the paper chip.
Compared with the prior art, the invention has the beneficial effects that:
1. the multilayer structure of the multilayer paper chip is particularly suitable for complex multistep biological analysis, such as enzyme-linked immunosorbent assay, signal amplification immunoreaction, nucleic acid detection and the like;
2. the fluid channel of the multilayer paper chip is not filled with cellulose, so that interlayer isolation is realized, and interlayer flow of fluid is realized only under the action of an external applied electric field or air flow, so that the detection process is controllable;
3. the manufacturing method realizes process automation, realizes automatic adhesion and alignment among different layers of paper, avoids the use of viscose and reduces human errors;
4. the manufacturing method adopts the digital mask to carry out photocuring, is simple and convenient and is easy for standardized manufacturing; after printing is finished, cleaning and drying uncured resin;
5. the fluid interlayer flowing method is controlled by using an electric field or airflow, so that the control process is programmed and automated, the Arduino software is used for programming the control program of the electric field or airflow, the liquid on the upper layer flows downwards in sequence and automatically according to the set time, the artificial participation is reduced, and the accuracy and the repeatability of the multi-step reaction can be improved.
Drawings
Fig. 1 is a schematic structural diagram of a paper chip according to a first embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of a portion A of a paper chip according to a first embodiment of the present invention;
FIG. 3 is a schematic top view of a paper chip according to a first embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a paper chip manufacturing apparatus according to a first embodiment of the present invention;
FIG. 5 is a diagram illustrating a first embodiment of a paper chip with ink droplets penetrating a fluid channel;
FIG. 6 is a graph showing the experimental effects of the second embodiment of the present invention after ink is dropped and before and after an electric field is applied;
FIG. 7 is a graph showing the experimental effect of the third embodiment of the present invention before and after applying the air flow after dropping the ink;
description of reference numerals: 1-a paper substrate; 2-a sample introduction zone; 3-a resin layer; 4-a base layer; 5-a fluid space; 52-detection zone; 51-a fluid channel; 6-a sample to be tested; 7-a light source; 8-a light transmission device; 9-a reaction vessel; 10-resin liquid; 11-platform.
Detailed Description
In order to more clearly understand the technical features, objects and effects of the present invention, the embodiments of the present invention will be described with reference to the accompanying drawings, but the scope of the present invention is not limited to the following.
The first embodiment is as follows:
as shown in fig. 1 to 3, the present invention provides a multi-layer paper chip structure, which comprises at least two layers of paper substrates 1, wherein at least one fluid space 5 is formed between the layers of the paper substrates 1 and the resin layer 3; the fluid space 5 comprises a sample introduction region 2, the sample introduction region 2 is connected with a fluid channel 51, and the fluid channel 51 is connected with at least one detection region 52; the sample introduction areas 2 of the same fluid space 5 of the paper substrate 1 are on the same line perpendicular to the paper substrate 1. The multilayer paper chip structure further comprises a base layer 4 on the surface of the uppermost paper substrate 1, wherein the base layer 4 is formed by resin printing. The material of the paper substrate 1 is filter paper. The paper substrate 1 is cellulose, and the fluid will diffuse to the detection area 52 along the fluid channel 51 in the single-layer paper substrate 1, but the fluid in the sample injection area 2 of a certain layer of the paper substrate 1 cannot cross the layer to the sample injection area 2 of the partition layer, so the sample 6 to be measured will diffuse only in a single layer without external force. The base layer 4 is used for making up the possible non-horizontal situation of the manufacturing device, the paper base material 1 is directly printed, if the paper base material 1 is inclined, the thickness of the printed resin layer 3 is uneven, and the resin is not cured at the thinnest place; the base layer 4 is also a resin layer in nature, but the resin layer is not used as a fluid channel, and only the lower surface of the resin layer is horizontal, and then the paper substrate 1 is placed on the base layer 4 to be almost placed on a horizontal plane. In practice, the detection area 52 is pre-filled with a substance that reacts with the sample 6 to be measured, and this reaction triggers a visually detectable change. The material of the paper substrate 1 is filter paper, and it is instructive that the material of the filter paper may be replaced by filter paper, chromatography paper, filter membrane, and ion exchange paper having similar effects.
The sample injection area of the paper substrate 1 is actually holes penetrating through the resin layer 3, the positions of the holes are determined when designing the paper chip structure, the holes are in a straight line, the purpose is to enable the sample 6 to be tested on the uppermost layer to penetrate through the paper substrate 1 flowing to all layers under the action of external electric field force or air flow, once the sample 6 to be tested is in the sample injection area 2 of the paper substrate 1 on a certain layer, the sample 6 to be tested can be diffused along the fluid channel 51 under the action of cellulose of the paper substrate 1 to reach the detection area 52, namely, liquid dropping on the front side of the paper substrate 1 can also be diffused to the back side of the paper substrate 1; on the other hand, where the paper base material 1 adheres with the resin, the diffusion is terminated, and the fluid is diffused only in the fluid passage 51 to which no resin adheres. The paper chip is characterized in that the paper chip is provided with at least one fluid space 5, each fluid space 5 is provided with a respective sample injection region 2, and the paper chip is particularly suitable for biological analysis with complex and multistep, such as enzyme-linked immunosorbent assay, signal amplification immunoreaction, nucleic acid detection and the like; after the sample introduction area 2 drops into the sample 6 to be detected, the control program of the electric field or the air flow is programmed by utilizing Arduino software, and the sample 6 to be detected sequentially flows into the fluid channel 51 from the sample introduction area 2 downwards automatically according to the set time, and then flows into the detection area 52.
As shown in fig. 4, the multilayer paper chip manufacturing apparatus, which is applied to manufacturing the multilayer paper chip structure, includes a light source 7, a light transmission device 8, a reaction vessel 9 and a platform 11; wherein, the light transmission device 8 is used for realizing and changing the shape of the transmission area of the light source 7; the light transmission device 8 is above the light source 7; a reaction vessel 9 containing a resin solution 10; the light transmission device 8 is arranged on the bottom surface of the reaction vessel 9, and the light source 7 can transmit into the reaction vessel 9 through the light transmission device 8; a platform 11, which can vertically move up and down, for placing a semi-finished product or a finished product of the paper chip in the process of printing the paper chip; when the stage 11 is at the lowest position, the distance between the stage and the bottom surface of the reaction vessel 9 is defined as the thickness of the resin to be coagulated. The light source 7 is an LED lamp. The light transmission means 8 is an LCD panel for receiving and displaying the shape of the computer designed transmission area through which the light source 7 can only enter the reaction vessel 9. The resin liquid 10 is a light-cured resin liquid, which can be polymerized by a photosensitizer in the resin liquid under the irradiation of light with a special wavelength, can rapidly generate physical and chemical changes in a short time, and further can be crosslinked and cured to form an insoluble coating, the coating thickness is larger as the irradiation time is longer, and the light source adopted in the embodiment is blue light with the wavelength of 405 nm. The pattern of the light transmission means 8 is divided into dark and light areas, the light in the dark areas being opaque and the light in the light areas being transparent, the dark and light areas of the light transmission means being displayed according to a pattern designed by a person in advance on a computer. The lower surface of the platform 11 is provided with tin foil paper, a coating film generated by curing the resin liquid 10 is adhered to the lower surface of the platform, the irradiation time determines the hardness of the printed resin, and the hardness influences the adhesion of the resin and the previous layer.
A method for manufacturing a multi-layer paper chip is applied to the method for manufacturing the multi-layer paper chip to manufacture a multi-layer paper chip structure, the total number of layers of a resin layer 3 is three (excluding a substrate layer 4, the same counting for the following layers) and the method comprises the following steps:
s1, downloading the designed shape of the transmission area from the computer by the light transmission device 8, and descending the platform 11 to the distance between the platform and the bottom surface of the reaction container 9, wherein the distance is the set thickness of the substrate layer 4; turning on the light source 7, exposing for 80s, and printing a blank substrate layer 4; after the blank substrate layer 4 is printed, the light source 7 is turned off, the platform 11 is lifted, and the substrate layer 4 is adhered to the lower surface of the platform 11;
s2, placing the paper substrate 1 in the reaction container 9, descending the platform 11 to a set position, turning on the light source 7, exposing for 80S, and printing the first resin layer 3; after printing the resin layer 3, turning off the light source 7, lifting the platform 11, and adhering the paper chip semi-finished product to the lower surface of the platform 11;
s3, placing the paper substrate 1 in the reaction container 9, downloading the designed shape of the transmission area from the computer by the light transmission device 8, descending the platform 11 to the set position, turning on the light source 7, exposing for 55S, and printing the resin layer 3; after the resin layer 3 is printed, the light source 7 is turned off, the platform 11 is lifted, and the paper chip semi-finished product is still adhered to the lower surface of the platform 11;
s4, repeating S3 until the printing of the third resin layer 3 is completed, wherein the exposure time is 55S.
Fig. 5 is a schematic diagram of the paper die of this embodiment after ink has been dropped through the fluid channel 51.
It should be noted that the light penetrates the paper chip 1, and the resin is cured on both sides of the upper and lower surfaces of the paper chip 1, and the cured resin adheres to the paper chip to form the resin layer 3. In addition, the resin cured during printing adheres to the lower surface of the stage 11 of aluminum alloy, and the adhesion of the resin to the stage 11 is not so strong when the base layer 4 is printed, so that the exposure time needs to be longer to enhance the adhesion of the both. When the second layer is printed after the base layer 4 is printed, the resin layer 3 cured on the upper surface of the paper chip 1 adheres to the base layer 4 more easily than the former, and therefore the exposure time of the base layer 4 is set to be slightly longer than that of the other resin layers 3.
The second embodiment is as follows:
on the basis of the first embodiment, the multilayer paper chip manufacturing method is used for manufacturing two resin layers 3, namely, two paper substrates 1, and the base layer 4 is exposed for 80s, and the exposure time of the first and second resin layers is 80s and 55s respectively.
The multilayer paper chip fluid interlayer flowing method is applied to the paper chip manufactured by the embodiment and comprises the following steps: and (3) dripping a sample 6 to be detected into the outermost sample injection region 2 of the paper chip, and applying an electric field on two sides of the sample injection region 2 of the paper chip. As shown in fig. 6, the left side of the figure is a front picture of the paper chip of the second embodiment before the electric field is applied after the ink is dropped on the paper chip, and the right side of the figure is a back picture of the paper chip after the electric field is applied; it should be noted that the ink in the sample injection region 2 on the uppermost layer of the front surface is limited in the region by the cured resin, and after the electric field is applied, the ink penetrates through the second layer of paper substrate 1, and can be seen more clearly from the back surface of the paper chip, and the ink is diffused to the detection region 52 through the flow channel 51.
The third concrete embodiment:
on the basis of the second embodiment, the method for controlling the flow between the fluid layers of the paper chip is changed, and the action of applying an electric field is changed into the action of applying air flow. The left side of the figure is a picture of the front side of the paper chip of the second embodiment before air flow is applied after ink is dripped, and the right side of the figure is a picture of the back side of the paper chip after air flow is applied; it should be noted that the ink in the sample injection region 2 on the uppermost layer of the front surface is limited in the region by the cured resin, and after the air flow, the ink penetrates through the second layer of paper substrate 1, and can be seen more clearly from the back surface of the paper chip, and the ink is diffused to the detection region 52 through the flow channel 51. It is to be understood that the gas flow is one of the mechanical forces that can be replaced by similar mechanical forces, such as pressing directly on the sample introduction area 2, and the sample 6 to be tested in the sample introduction area 2 is directly pressed to the next layer.
It can be shown that the multilayer structure of the multilayer paper chip is particularly suitable for bioanalysis of complex multiple steps on paper, such as enzyme-linked immunosorbent assay, signal amplification immunoreaction, nucleic acid detection and the like; the fluid channel 51 of the multilayer paper chip is not filled with cellulose, so that interlayer isolation is realized, and interlayer flow of fluid is realized only under the action of an external applied electric field or air flow, so that the detection process is controllable; the manufacturing method realizes process automation, realizes automatic adhesion and alignment among different layers of paper, avoids the use of viscose and reduces human errors; the manufacturing method adopts the digital mask to carry out photocuring, is simple and convenient and is easy for standardized manufacturing; after printing is finished, cleaning and drying uncured resin; the fluid interlayer flowing method is controlled by using an electric field or airflow, so that the control process is programmed and automated, the Arduino software is used for programming the control program of the electric field or airflow, the liquid on the upper layer flows downwards in sequence and automatically according to the set time, the artificial participation is reduced, and the accuracy and the repeatability of the multi-step reaction can be improved.
The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention, and it is therefore to be understood that the invention is not limited by the scope of the appended claims.
Claims (3)
1. The multilayer paper chip fluid interlayer flowing method is applied to a multilayer paper chip structure and is characterized in that the multilayer paper chip structure comprises at least two layers of paper base materials (1), and at least one fluid space (5) formed by a resin layer (3) and the paper base materials (1) is arranged between the layers; the fluid space (5) comprises a sample introduction area (2), the sample introduction area (2) is connected with a fluid channel (51), and the fluid channel (51) is connected with at least one detection area (52); the sample injection areas (2) of the same fluid space (5) are on the same straight line vertical to the paper substrate (1); the multilayer paper chip structure further comprises a base layer (4) arranged on the surface of the uppermost paper substrate (1), wherein the base layer (4) is formed by resin printing; the flow method between the fluid layers comprises the following steps: dripping a sample (6) to be tested into the outermost sample injection region (2) of the paper chip, and applying an electric field on two sides of the sample injection region (2) of the paper chip or applying air flow on one side of the sample injection region (2) of the paper chip; the control program of the electric field or the air flow is programmed, and the liquid on the upper layer flows downwards in sequence and automatically according to the set time.
2. The multilayer paper chip fluid laminar flow method according to claim 1, characterized in that: the material of the paper base material (1) is filter paper.
3. A multilayer paper chip manufacturing method applied to a multilayer paper chip manufacturing apparatus, characterized by comprising:
a light source (7), and
-light transmission means (8) for realising and modifying the shape of the transmission area of the light source (7); the light transmission device (8) is arranged above the light source (7);
a reaction vessel (9) containing a resin solution (10); the light transmission device (8) is arranged on the bottom surface of the reaction container (9), and the light source (7) can transmit into the reaction container (9) through the light transmission device (8);
the platform (11) can vertically move up and down and is used for placing a semi-finished product or a finished product of the paper chip in the process of printing the paper chip; when the platform (11) is at the lowest position, the distance between the platform and the bottom surface of the reaction container (9) is used as the thickness of the resin condensation;
the manufacturing method includes the steps of:
s1, downloading the designed shape of the transmission area from the computer by the light transmission device (8), and descending the platform (11) to the distance between the platform and the bottom surface of the reaction container (9) which is the set thickness of the substrate layer (4); turning on a light source (7), exposing according to set time, and printing a blank substrate layer (4); after the blank substrate layer (4) is printed, the light source (7) is turned off, the platform (11) rises, and the substrate layer (4) is adhered to the lower surface of the platform (11);
s2, placing the paper base material (1) in a reaction container (9), descending the platform (11) to a set position, turning on the light source (7), exposing according to set time, and printing the first resin layer (3); after the resin layer (3) is printed, the light source (7) is turned off, the platform (11) rises, and the paper chip semi-finished product is adhered to the lower surface of the platform (11);
s3, placing the paper substrate (1) in a reaction container (9), downloading the designed shape of the transmission area from a computer by the light transmission device (8), descending the platform (11) to a set position, turning on the light source (7), exposing according to a set time, and printing the resin layer (3); after the resin layer (3) is printed, the light source (7) is turned off, the platform (11) rises, and the paper chip semi-finished product is still adhered to the lower surface of the platform (11);
s4, repeat S3 until printing of all resin layers (3) is completed.
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