CN108964781A - Multichannel coherent Raman scattering Optical devices and imaging system - Google Patents
Multichannel coherent Raman scattering Optical devices and imaging system Download PDFInfo
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- CN108964781A CN108964781A CN201810915540.0A CN201810915540A CN108964781A CN 108964781 A CN108964781 A CN 108964781A CN 201810915540 A CN201810915540 A CN 201810915540A CN 108964781 A CN108964781 A CN 108964781A
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04B—TRANSMISSION
- H04B10/00—Transmission systems employing electromagnetic waves other than radio-waves, e.g. infrared, visible or ultraviolet light, or employing corpuscular radiation, e.g. quantum communication
- H04B10/50—Transmitters
- H04B10/516—Details of coding or modulation
- H04B10/54—Intensity modulation
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04B—TRANSMISSION
- H04B10/00—Transmission systems employing electromagnetic waves other than radio-waves, e.g. infrared, visible or ultraviolet light, or employing corpuscular radiation, e.g. quantum communication
- H04B10/25—Arrangements specific to fibre transmission
- H04B10/2507—Arrangements specific to fibre transmission for the reduction or elimination of distortion or dispersion
- H04B10/2537—Arrangements specific to fibre transmission for the reduction or elimination of distortion or dispersion due to scattering processes, e.g. Raman or Brillouin scattering
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04B—TRANSMISSION
- H04B10/00—Transmission systems employing electromagnetic waves other than radio-waves, e.g. infrared, visible or ultraviolet light, or employing corpuscular radiation, e.g. quantum communication
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- H04B10/503—Laser transmitters
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Abstract
The present invention relates to a kind of multichannel coherent Raman scattering Optical devices and imaging systems, including beam splitter, the first optical module, the second optical module, light intensity modulator and the first bundling device；Seed light is split transmission by beam splitter；Seed light is converted pump light by first optical module；Seed light is converted the mutually different stokes light of wavelength by second optical module；Light intensity modulator is modulated the light intensity of stokes light, each stokes light exports to the first bundling device in the form of light intensity power is staggered and carries out conjunction beam, the coherent Raman scattering signal that the road Shi Ge stokes light and pump light excite the vibration mode of a variety of specific moleculars to generate, realize that multichannel in situ is imaged simultaneously, the working efficiency of improving optical imaging system, replace expensive highly-sensitive detector array, additionally it is possible to avoid multi beam high-peak power light while excite sample that nonlinear optics is caused to be saturated.
Description
Technical field
The present invention relates to optical technical fields, more particularly to a kind of multichannel coherent Raman scattering Optical devices and multi-pass
Road coherent Raman scattering micro imaging system.
Background technique
Coherent Raman scattering spectral technique is the important technology in optical technical field, including observation of stimulated Raman scattering skill
Art, coherent antistockes Raman spectroscopy technology, Raman induction Kerr effect etc., are mainly excited using ultra-short pulse laser
The non linear optical signal of sample itself obtains the micro-imaging of sample, is somebody's turn to do then by applying laser scanning and microtechnic
Technical application in bio-imaging and Molecular Detection field, have exempt from chemical dye label, the imaging of biomolecule specificity, it is three-dimensional at
The advantages such as picture, are of great value in medical domains such as histotomy imaging samples, cell imaging, human body dynamic imagings.
When the Characteristic Raman peak match of the difference on the frequency and sample of two beams or the above exciting light, excited Raman may be implemented and dissipate
The radiation of signal is penetrated, so that the optical signalling that sample generates is greatly enhanced.That reports at present is used to generate phase dry drawing
The technology of graceful scattered signal mainly includes three kinds, the first is relevant as generating using the solid state laser of two PGC demodulations
The pump light and stokes light of Raman scattering；Second is to be equipped with optical parametric oscillator or light using ultrashort pulse laser
Parameter amplifier is learned, pump light and stokes light are generated；The third is equipped with by ultrashort pulse Solid State Laser or optical-fiber laser
Non-linear photon crystal optical fiber generates pump light and stokes light.
Wherein, use the main problem of the scheme of solid state laser big for system bulk, structure is complicated, and it is expensive, and
And optical path is free optical path, stability is poor.
On the other hand, the supercontinuum power that pumped nonlinear photonic crystal fiber generates is lower, the phase dry drawing of generation
Graceful scattered signal is very weak, highly sensitive detector array can only be used to be detected, and be only applicable to the anti-stoke that is concerned with
This Raman scattering micro-imaging.It can make an uproar comprising background caused by non resonant signal in coherent anti-stokes raman scattering spectrum
Sound is unfavorable for spectrum analysis, and highly sensitive detector array such as electronics enhancing charge-coupled device price is extremely expensive,
The technical costs is high.For stimulated Raman scattering, does not include ambient noise in spectrum and detector price is lower, however,
Stimulated Raman scattering micro-imaging cannot achieve using the measuring technique of detector array at present.
In short, coherent Raman scattering micro-imaging technique direction is the system, reduction that design structure is compact, stable at present
Cost realizes multichannel or high light spectrum image-forming.
Summary of the invention
Based on this, for different molecular vibration mode, realizes that multichannel coherent Raman scattering signal is imaged simultaneously, promote system
The working efficiency of system, and replace expensive highly-sensitive detector array, a kind of multichannel coherent Raman scattering is provided
Optical devices and multichannel coherent Raman scattering micro imaging system.
A kind of multichannel coherent Raman scattering Optical devices, comprising: beam splitter, the first optical module, the second optical module,
Light intensity modulator and the first bundling device；Wherein,
The beam splitter, for seed light beam splitting extremely to be pumped to branch and at least two-way Stokes branch transmission；
First optical module, for converting pumping light output to described first for the seed light of the pumping branch
Bundling device；
Second optical module, it is different for converting wavelength for the seed light of each Stokes branch
Stokes light；
The light intensity modulator is modulated for the light intensity to the stokes light, so that each stoke
This light exports in the form of light intensity power is staggered to first bundling device；
First bundling device, for the pump light and each stokes light to be carried out conjunction beam.
Above-mentioned multichannel coherent Raman scattering Optical devices, beam splitter can receive seed light and by its beam splitting to pump branch
It is transmitted in road and at least two-way Stokes branch, the first optical module is converted into the seed light transmitted on branch is pumped
For generating the pump light of coherent Raman scattering signal, the kind that second optical module will transmit on each Stokes branch
Sub-light is converted into the mutually different stokes light of frequency, and light intensity modulator is modulated the light intensity of the stokes light, makes
By light intensity it is strong and weak it is staggered in the form of export to the first bundling device and carry out conjunction beam so that the light intensity power closed after beam is staggered
The coherent Raman scattering signal that each road stokes light and pump light excite the vibration mode of a variety of specific moleculars to generate is realized more
Channel coherent Raman scattering signal is imaged simultaneously, the working efficiency of improving optical imaging system, replaces expensive highly sensitive
It is caused non-linear when spending detector array, but also can be avoided multi beam high-peak power stokes light while exciting sample
Optics saturation, improves the quality of coherent Raman scattering signal.
It in one embodiment, further include closing beam set on second between second optical module and light intensity modulator
Device；Wherein, second bundling device for the mutually different each stokes light of wavelength to be carried out conjunction beam, and exports
To the light intensity modulator.
It in one embodiment, further include set on the third bundling device between the light intensity modulator and the first bundling device；
Wherein, the quantity of the light intensity modulator is multiple, is respectively arranged on each Stokes branch road；The third closes beam
Device for the stokes light of each light intensity modulator output to be carried out conjunction beam, and is exported to the first bundling device.
In one embodiment, first optical module includes times frequency module set on the pumping branch road；Wherein,
Described times of frequency module, for exporting seed light progress frequency multiplication for the pump light.
In one embodiment, first optical module further includes being set to the beam splitter and the again tune between frequency module
Humorous module；Wherein, the tuner module is tuned for the seed light by the beam splitter beam splitting to pumping branch, and defeated
Out to times frequency module.
In one embodiment, the tuner module successively includes photonic crystal fiber and the first narrow band filter；Wherein,
The photonic crystal fiber, the seed light for that will pump branch carries out frequency expansion, and exports to first narrowband
Optical filter；First narrow band filter, for filtering out the seed light of assigned frequency from the seed light after frequency expansion,
And it exports to described times of frequency module.
In one embodiment, first optical module further includes the first fiber amplifier and the first beam collimator；
Wherein, first fiber amplifier and the first beam collimator be sequentially arranged in first narrow band filter and times frequency module it
Between；The seed light of first fiber amplifier, the assigned frequency for filtering out first narrow band filter carries out
Amplification, and export to first beam collimator；First beam collimator, for by the amplified seed light into
Row collimation, and export to described times of frequency module.
In one embodiment, second optical module successively include the second narrow band filter, the second fiber amplifier,
Second beam collimator and light path adjustment mechanism；Wherein, second narrow band filter, for from the beam splitter beam splitting to pump
The stokes light of assigned frequency is filtered out in the seed light of Pu branch road, and is exported to second fiber amplifier；Institute
The second fiber amplifier is stated, for amplifying the stokes light, and is exported to second beam collimator；It is described
Second beam collimator for collimating the stokes light of amplification, and is exported to the light path adjustment mechanism；Institute
Light path adjustment mechanism is stated, the light path for the stokes light to the collimation is adjusted, so that this described each described support
The light path of the stokes light of gram this branch is consistent with the pumping light path of pump light of branch road.
In one embodiment, a kind of multichannel coherent Raman scattering micro imaging system is provided, including what is be sequentially arranged
Multichannel coherent Raman scattering Optical devices described in any one of optical fiber laser, optoisolator, as above embodiment, laser vibration
Mirror, microscope and optical signal detection equipment；Wherein,
The optical fiber laser, for seed light to be input to the Optical devices by optoisolator；
The multichannel coherent Raman scattering Optical devices, are used to generate coherent Raman for converting the seed light to
The pump light of scattered signal and at least two beam stokes lights, and close beam and export to the laser galvanometer；
The laser galvanometer, for the pump light and stokes light that close beam to be input in the microscope, with
Make to generate coherent Raman scattering signal at the microscopical sample；
The optical signal detection equipment, for detecting the coherent Raman scattering signal and to the coherent Raman scattering signal
It is imaged.
Above-mentioned multichannel coherent Raman scattering micro imaging system, beam splitter can receive the seed of optical fiber laser generation
Its beam splitting is simultaneously extremely pumped branch and at least transmitted in two-way Stokes branch by light, and the first optical module will pump branch
The seed light of upper transmission is converted into the pump light for generating coherent Raman scattering signal, and the second optical module is by each stoke
The seed light transmitted on this branch is converted into the mutually different stokes light of frequency, and light intensity modulator is to the stokes light
Light intensity is modulated, be allowed to by light intensity it is strong and weak it is staggered in the form of export to the first bundling device and carry out conjunction beam so that after closing beam
The strong and weak staggered each road stokes light of light intensity can be focused at microscopical sample and make by laser galvanometer with pump light
It obtains the sample and generates coherent Raman scattering signal, then the coherent Raman scattering signal is detected by optical signal detection equipment and is carried out
Imaging, caused nonlinear optics saturation, is improved when can be avoided multi beam high-peak power stokes light while exciting sample
The quality of coherent Raman scattering signal, additionally it is possible to while the vibration mode of a variety of specific moleculars of sample is excited, generate at least two
Kind coherent Raman scattering signal carries out multi channel imaging in situ, and using optical fiber laser as coherent Raman scattering signal
Seed light source so that optical system is more compact, structure is simpler, reduces costs reduction, improves optical system
Stability.
In one embodiment, the optical signal detection equipment successively includes optical filter, photodetector and signal processing
Device；Wherein, the optical filter for filtering out the veiling glare of the coherent Raman scattering signal, and will filter out the phase after veiling glare
Dry Raman scattering signal is exported to the photodetector；The photodetector is used for the coherent Raman scattering signal
It is converted into corresponding electric signal, and is exported to the signal processor；The signal processor, for carrying out the electric signal
Imaging.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of multichannel coherent Raman scattering Optical devices and imaging system in one embodiment；
Fig. 2 is the modulation principle schematic diagram of binary channels stokes light in one embodiment.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that specific embodiment described herein is only used to explain the present invention, not
For limiting the present invention.
It should be noted that term involved in the embodiment of the present invention " first second third " be only be that difference is similar
Object, do not represent the particular sorted for object, it is possible to understand that ground, " Yi Er third " can be in the case where permission
Exchange specific sequence or precedence.It should be understood that the object that " first second third " is distinguished in the appropriate case can be mutual
It changes, so that the embodiment of the present invention described herein can be real with the sequence other than those of illustrating or describing herein
It applies.
In one embodiment, a kind of Optical devices are provided, which can be used for exciting coherent Raman scattering
Signal is the structural representation of multichannel coherent Raman scattering Optical devices and imaging system in one embodiment with reference to Fig. 1, Fig. 1
Figure, which may include: beam splitter 200, the first optical module 300, the second optical module 400, light intensity modulator
500 and first bundling device 600.
Beam splitter 200 is the optics for being split the laser that such as laser light source 100 exports according to certain energy proportion
Device, so the present embodiment can be by the reception output of laser light source 100 of beam splitter 200 for exciting coherent Raman scattering to believe
Number seed light, and by the beam splitter 200 by received seed light according to certain energy ratio beam splitting to pumping branch and extremely
Few two-way Stokes branch is transmitted.Wherein, doped rare earth element glass optical fiber can be used as gain in laser light source 100
Medium, these rare earth elements may include neodymium, erbium, ytterbium, holmium or thulium etc., and fiber pulse laser light source can be by solid laser
Oscillator substitution, range of pulse repetition frequency includes 1kHz to 100MHz.
First optical module 300 can be set to pumping branch road, be mainly used for turning beam splitting to the seed light of pumping branch
Pump light is turned to, and the pump light converted is output to the first bundling device.
Second optical module 400 can be set to each road Stokes branch road, be mainly used for each road Stokes branch
Seed light transform into the mutually different stokes light of wavelength.It is illustrated by taking two-way Stokes branch as an example, second
Optical module 400 can be by wherein the frequency translation of the seed light of Stokes branch be first frequency all the way, and by another way
The frequency translation of seed light be the second frequency different from first frequency, can be in the case where multichannel Stokes branch
The mutually different stokes light of frequency is converted by each road seed light, forms the stokes light of the different frequency of multichannel.
Light intensity modulator 500 is mainly used for being modulated the light intensity of stokes light, which can be right
The light of different wave length carries out independent modulation, and the present embodiment light intensity modulator 500 can export each Stokes branch not
The light intensity of the stokes light of same frequency is modulated respectively, is output to it in first bundling device in the form of light intensity power is staggered.
It is illustrated by taking binary channels stokes light as an example, is the tune of binary channels stokes light in one embodiment with reference to Fig. 2, Fig. 2
Schematic illustration processed, since the frequency of the stokes light in two channels is different, so light intensity modulator can be adjusted with first
The stokes light in the one of channel of frequency modulation(PFM) processed, and with second modulating frequency different from the first modulating frequency to another
The stokes light in a channel is modulated, so that the light intensity of the stokes light of two channels output is wrong each other on a timeline
It opens to export to the pump light of the first bundling device and pumping branch output and be concerned with, the coherent light that output intensity staggeredly changes to subsequent sample
In product, the damage for causing sample when synchronization is input to the sample of subsequent excitation Raman scattering is concentrated on to avoid high power laser light
Bad and non-linear saturation and cause the quality of coherent Raman scattering signal to decline.Optionally, light intensity modulator 500 can use sound
Optic tunable filter or electrooptic modulator, modulation frequency range may include 1kHz to 100MHz.
First bundling device 600 is mainly used for that the pump light and this each after intensity modulation of branch output will be pumped
Lentor light carries out conjunction beam, generates the coherent light for exciting Raman scattering signal, due in the coherent light different frequency this
The light intensity of lentor light staggeredly changes, so the coherent light excitation sample exported using the first bundling device 600 is excited to draw
Graceful scattered signal can be avoided high power laser light to the damage of sample and the saturation of nonlinear properties, improve coherent Raman scattering
The quality of signal.Wherein, the first bundling device 600 may include dichroic mirror, laser beam splitter mirror or polarizing beam splitter mirror etc..
Above-mentioned multichannel coherent Raman scattering Optical devices, beam splitter can receive seed light and by its beam splitting to pump branch
It is transmitted in road and at least two-way Stokes branch, the first optical module is converted into the seed light transmitted on branch is pumped
For generating the pump light of coherent Raman scattering signal, the kind that second optical module will transmit on each Stokes branch
Sub-light is converted into the mutually different stokes light of frequency, and light intensity modulator is modulated the light intensity of the stokes light, makes
By light intensity it is strong and weak it is staggered in the form of export to the first bundling device and carry out conjunction beam so that the light intensity power closed after beam is staggered
The coherent Raman scattering signal that each road stokes light and pump light excite the vibration mode of a variety of specific moleculars to generate is realized more
Channel coherent Raman scattering signal is imaged simultaneously, the working efficiency of improving optical imaging system, replaces expensive highly sensitive
It is caused non-linear when spending detector array, but also can be avoided multi beam high-peak power stokes light while exciting sample
Optics saturation, improves the quality of coherent Raman scattering signal.
It in one embodiment, can also include set on second between the second optical module 400 and light intensity modulator 500
Bundling device 700.
In the present embodiment, the second bundling device 700 is mainly used for receiving the stokes light of the road output of each road Stokes,
The mutually different each road stokes light of wavelength is subjected to conjunction beam, and the road He Shuge stokes light is output to intensity modulation
Device 500 carries out intensity modulation.The mutually different each road stokes light of the wavelength for closing beam is output to intensity modulation by the present embodiment
Device 500, which carries out intensity modulation, can save the quantity of light intensity modulator 500, be equally reached the Stokes to different wave length
The effect that light is modulated also makes optical path more compact, saves the arrangement space of optical system.
It in one embodiment, can also include being closed set on the third between light intensity modulator 500 and the first bundling device 600
Beam device.
In the present embodiment, the quantity of light intensity modulator 500 can be multiple, and each light intensity modulator 500 is respectively arranged on respectively
A Stokes branch road, carries out independent modulation with the stokes light respectively to each road Stokes branch road.Third closes beam
Device be mainly used for the mutually different stokes light of wavelength for exporting the light intensity modulator 500 of each road Stokes branch road into
Row closes beam, then the stokes light after conjunction beam is output to the first bundling device 600.
Mainly light intensity modulator 500 is respectively set on each Stokes branch road in the technical solution of the present embodiment, with
It realizes and independent modulation is carried out to the stokes light of each road Stokes branch, each road for recycling third bundling device that will modulate
Stokes light carries out closing beam output, is modulated due to using multiple light intensity modulators, and institute in this way can be more
Accurately the light intensity of each road stokes light is modulated.
In one embodiment, the first optical module may include times frequency module 310 set on pumping branch road.
In the present embodiment, times frequency module 310 refers to the optical element that process of frequency multiplication is carried out to the frequency of light, be mainly used for by
The seed light of beam splitter beam splitting to pumping branch carries out frequency multiplication output as the pump light for exciting Raman scattering signal.It is optional
, times frequency module 310 may include focusing optic 311, frequency-doubling crystal 312 and optically filtering piece 313, frequency-doubling crystal 312
Set on the focal point of focusing optic 311, focusing optic 311 is mainly used for focusing to seed light into frequency-doubling crystal 312,
So that the seed light of focusing is carried out frequency multiplication output by the frequency-doubling crystal 312.Wherein, focusing optic 311 may include two sides
It is respectively arranged on the convergent lens or two sides concave mirror of 312 two sides of frequency-doubling crystal, frequency-doubling crystal 312 may include barium borate crystalline substance
Body, lithium triborate crystal, period polarized superlattices lithium tantalate, period polarized magnesium oxide doped lithium niobate crystal or periodical pole
Change lithium columbate crystal.
The present embodiment be mainly consider seed light frequency would generally by 100 type of laser light source limitation and cannot
Cover frequency required for pump light, the technical solution of the present embodiment can be more convenient neatly be by seed light is wavelength converting
The pump wavelength needed.
In one embodiment, further, the first optical module 300 can also include being set to beam splitter 200 and frequency multiplication
Tuner module 320 between module 310.
Wherein, tuner module 320 refers to the optical element being adjusted to the frequency of seed light, is mainly used for beam splitter
The seed light of 200 beam splitting to pumping branch is tuned, and by the seed light output after tuning to frequency module 310 again, optionally,
Optical parametric oscillator or optical parametric amplifier can be used in tuner module 320.
Under normal circumstances, seed light is carried out only with frequency module 310 again that the frequency of pump light can't be obtained, this be because
The seed light frequency exported for laser light source 100 is relatively simple, so the pump light obtained even across frequency module again
Frequency also single frequency, under the application scenarios for needing to be adjusted pumping light frequency, single seed light frequency is often
It is difficult to meet demand.The present embodiment is first adjusted the frequency of seed light using tuner module 320, recycles times frequency module
310 progress process of frequency multiplication obtain pump light, so that when needing to be adjusted pumping light frequency, it is only necessary to adjust tuner module
320 can be quickly obtained the pump light of corresponding frequencies by frequency module 310 again, further improve the flexible of the Optical devices
Property.
In one embodiment, optionally, tuner module 320 can successively include that photonic crystal fiber 321 and first is narrow
Band optical filter 322.
Wherein, photonic crystal fiber 321 is mainly used for beam splitter beam splitting to the seed light of pumping branch carrying out frequency spectrum exhibition
Width generates the supercontinuum such as 500 nanometers to 1700 nanometers, and selects specific frequency by the first narrow band filter 322
Seed light output is to frequency module 310 again.
The present embodiment further uses photonic crystal fiber 321 and carries out frequency expansion processing to seed light, makes its generation
Supercontinuum can be extracted from the frequency spectrum of supercontinuum after supercontinuum generation by the first narrow band filter
The seed light of specific frequency out.When needing to be adjusted pumping light frequency, it is only necessary to adjust the choosing of the first narrow band filter 322
The light of specific frequency is selected, the pump light of corresponding frequencies can be quickly obtained by frequency module 310 again, further improves this
The flexibility of Optical devices.
In one embodiment, further, first assembly 300 can also include the first fiber amplifier 330 and first
Beam collimator 340.
Wherein, the first fiber amplifier 330 and the first beam collimator 340 be sequentially arranged in the first narrow band filter 322 with
Between times frequency module 310, the first fiber amplifier 330 is mainly used for the assigned frequency for filtering out the first narrow band filter 322
Seed light carries out power amplification, and output is to the first beam collimator 340, then by the first beam collimator 340 by the power amplification
Seed light collimated, the seed light after collimation is subjected to frequency multiplication by frequency module 310 again, it is relevant that frequency doubled light can be used as generation
The pump light of Raman scattering signal.Optionally, the first fiber amplifier 330 can be dilute using doping neodymium, erbium, ytterbium, holmium or thulium etc.
Gain media of the earth elements as the amplifier.
Seed light can be amplified collimation by the present embodiment, on the one hand improved the energy of pump light, on the other hand may be used also
So that the seed light collimation of power amplification is input to times frequency module 310, it is ensured that times frequency module 310 can be more effectively to seed
Light carries out the pump light that frequency multiplication generates high quality.
In one embodiment, the second optical module 400 can successively include the second narrow band filter 410, the second optical fiber
Amplifier 420, the second beam collimator 430 and light path adjustment mechanism 440.
It should be noted that the second optical module 400 can be set to each Stokes branch road, the present embodiment mainly with
Wherein a Stokes branch is illustrated the second optical module 400.Wherein, the second narrow band filter 410 is mainly used for
The wavelength for selecting the stokes light of each Stokes branch can be used for the seed from beam splitter beam splitting to pumping branch road
The stokes light of assigned frequency is filtered out in light, and is output to the second fiber amplifier 420, uses the second fiber amplifier 420
Power amplification is carried out to stokes light, and is output to the second beam collimator 430, which can use
It is collimated in by the stokes light of amplification, and the stokes light after collimation is output in light path adjustment mechanism 440,
The light path of stokes light for collimation is accurately adjusted, so that the light of the stokes light of each Stokes branch
Journey is consistent with the pumping light path of pump light of branch road, wherein light path adjustment mechanism 440 may include 441 He of linear translation platform
The reflecting mirror being disposed vertically, hollow retroreflector, roof prism, retroreflection rib can be used in retroreflector 442, the retroreflector 442
The elements such as mirror or right-angle prism are realized.
Technical solution provided in this embodiment can respectively to be filtered per stokes light all the way, amplify, collimate with
And adjustment light path, to keep the Stokes optical signal obtained stronger, signal-to-noise ratio is higher, and can be same with pump light collective effect
The coherent Raman scattering signal of Shi Jifa multichannel.
In one embodiment, a kind of multichannel coherent Raman scattering micro imaging system is provided, is with reference to Fig. 1, Fig. 1
The structural schematic diagram of multichannel coherent Raman scattering Optical devices and imaging system in one embodiment, the multichannel coherent Raman
Scattering micro imaging system may include: the optical fiber laser 100 being sequentially arranged, optoisolator 110, as above any one embodiment
Multichannel coherent Raman scattering Optical devices 10, laser galvanometer 120, microscope 130 and the optical signal detection equipment 140.
In the present embodiment, optical fiber laser 100 can be used for generating this support of the multichannel of multichannel coherent Raman scattering imaging
The seed light is input to multichannel coherent Raman scattering optics by optoisolator 110 by the seed light of Ke Si light and pump light
In device 10, wherein optoisolator 110 is for eliminating back reflected laser to fiber pulses laser lights such as optical fiber lasers 100
The adverse effect in source.
Multichannel coherent Raman scattering Optical devices 10 can be used for converting the seed light that optoisolator 110 exports to
For generating the pump light and at least two beam stokes lights of coherent Raman scattering signal, and closes beam and export to laser galvanometer
In 120, enter 130 main body of microscope via laser galvanometer scanning system.
Laser galvanometer 120, for multichannel coherent Raman scattering Optical devices 10 to be closed to the pump light and Si Tuo of beam output
Ke Si light is input in microscope 130, so as to generate coherent Raman scattering signal at the sample of microscope 130.Specifically,
It is confocal in the focal plane of lens of microscope 130 that laser galvanometer 120 makes pump light and stokes light, so that inspiring at sample
Coherent Raman scattering non linear optical signal.Wherein, microscope 130 can be epi-illuminating microscope or transmitted light microscope,
Or commercial microscope or self-control microscope, the range of the amplification factor for the object lens that microscope 130 is equipped with include 10 times
To 100 times, the range of numerical aperture includes 0.1 to 1.49.
Optical signal detection equipment 140 is the equipment that optical signal is acquired and is handled, and is mainly used for detecting coherent Raman
Scattered signal is simultaneously imaged the coherent Raman scattering signal, which can be collected by condenser
The coherent Raman scattering non linear optical signal excited at sample.
The present embodiment keeps system structure tighter using ultrashort pulse fiber laser as the excitaton source of coherent Raman scattering
It gathers, stability is higher, and greatly reduces the cost of light source, using the modulation respectively of multi beam stokes light, for a variety of
The different vibration modes of specific molecular realize the coherent Raman scattering micro-imaging of binary channels or more multi-channel synchronous, replace
Expensive highly-sensitive detector array, and the saturation of non linear optical signal is avoided, the work of significant increase system
Efficiency.
Optionally, optical signal detection equipment 140 can successively include optical filter 141, photodetector 142 and signal processing
Device 143.Wherein, optical filter 141 can be used for filtering out the veiling glare of coherent Raman scattering signal, and will filter out the phase dry drawing after veiling glare
Graceful scattered signal is exported to photodetector 142；Photodetector 143 can be used for converting phase for coherent Raman scattering signal
The electric signal answered, and corresponding electric signal is exported to signal processor, the signal processor is for electric signal to be imaged
Processing, for stimulated Raman scattering micro-imaging, signal processor can carry out locking phase by the signal to two kinds of modulating frequencies respectively
Amplification, so that the non linear optical signal of different frequency is extracted, to realize the coherent Raman imaging of multi-channel synchronous.Similarly
It is applicable to coherent anti-stokes raman scattering imaging and Raman induction Kerr effect.
For stimulated Raman scattering signal, photodetector can be detected using optics biased detector, photodiode
Device or detector array place optical filter before photodetector and filter out the stokes light modulated, then using lock
Phase amplifier, data collecting card or averager extract stimulated Raman scattering signal.And for Raman induction Kerr effect letter
Number, same detection mode can be used, increases before detector and places an analyzer.
For coherent anti-stokes raman scattering signal, optical detector can use highly-sensitive detector, such as
Photomultiplier tube, single photon counting multiplier tube, single photon counting avalanche diode detector, charge-coupled device or electronics
Enhance charge-coupled device etc., short pass filter is placed before detector and all filters out stokes light and pump light, then
Using the circuit measurings coherent anti-Stokes Raman signal such as lock-in amplifier, data collecting card or photon counter.
Above-mentioned multichannel coherent Raman scattering micro imaging system can be applied to label-free bio-imaging and molecule inspection
It surveys, such as histotomy imaging samples, cell imaging, human body dynamic imaging；Wherein, beam splitter can receive optical fiber laser production
Its beam splitting is simultaneously extremely pumped branch and at least transmitted in two-way Stokes branch by raw seed light, and the first optical module will
The seed light transmitted on pumping branch is converted into the pump light for generating coherent Raman scattering signal, and the second optical module will be each
The seed light transmitted on a Stokes branch is converted into the mutually different stokes light of frequency, and light intensity modulator is to this support
The light intensity of Ke Si light is modulated, be allowed to by light intensity it is strong and weak it is staggered in the form of export to the first bundling device and carry out conjunction beam, to make
The strong and weak staggered each road stokes light of light intensity after beam must be closed can be focused on microscopical with pump light by laser galvanometer
Make the sample generate coherent Raman scattering signal at sample, the vibration mode of a variety of specific moleculars of sample can be detected simultaneously
The coherent Raman scattering signal of generation realizes that multichannel in situ is imaged simultaneously, replaces expensive highly-sensitive detector battle array
Column, the working efficiency of significant increase system can also avoid multi beam high-peak power stokes light while cause when exciting sample
Nonlinear optics saturation, improve coherent Raman scattering signal quality, and using optical fiber laser as coherent Raman dissipate
The seed light source of signal is penetrated, so that optical system is more compact, structure is simpler, reduces costs reduction, improves optics
The stability of system.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of multichannel coherent Raman scattering Optical devices characterized by comprising beam splitter, the first optical module, second
Optical module, light intensity modulator and the first bundling device；Wherein,
The beam splitter, for seed light beam splitting extremely to be pumped to branch and at least two-way Stokes branch transmission；
First optical module, for converting pumping light output to the first conjunction beam for the seed light of the pumping branch
Device；
Second optical module, for by the seed light of each Stokes branch be converted into wavelength it is mutually different this
Lentor light；
The light intensity modulator is modulated for the light intensity to the stokes light, so that each stokes light
It exports in the form of light intensity power is staggered to first bundling device；
First bundling device, for the pump light and each stokes light to be carried out conjunction beam.
2. multichannel coherent Raman scattering Optical devices according to claim 1, which is characterized in that further include set on described
The second bundling device between second optical module and light intensity modulator；Wherein,
Second bundling device for the mutually different each stokes light of wavelength to be carried out conjunction beam, and is exported to institute
State light intensity modulator.
3. multichannel coherent Raman scattering Optical devices according to claim 1, which is characterized in that further include set on described
Third bundling device between light intensity modulator and the first bundling device；Wherein, the quantity of the light intensity modulator is multiple, is set respectively
In each Stokes branch road；
The third bundling device for the stokes light of each light intensity modulator output to be carried out conjunction beam, and exports extremely
First bundling device.
4. multichannel coherent Raman scattering Optical devices according to claim 1, which is characterized in that the first optics group
Part includes times frequency module set on the pumping branch road；Wherein,
Described times of frequency module, for exporting seed light progress frequency multiplication for the pump light.
5. multichannel coherent Raman scattering Optical devices according to claim 4, which is characterized in that the first optics group
Part further includes being set to the beam splitter and the again tuner module between frequency module；Wherein,
The tuner module is tuned for the seed light by the beam splitter beam splitting to pumping branch, and exports to described
Times frequency module.
6. multichannel coherent Raman scattering Optical devices according to claim 5, which is characterized in that the tuner module according to
Secondary includes photonic crystal fiber and the first narrow band filter；Wherein,
The photonic crystal fiber, the seed light for that will pump branch carries out frequency expansion, and exports to described first
Narrow band filter；
First narrow band filter, for filtering out the seed light of assigned frequency from the seed light after frequency expansion, and
It exports to described times of frequency module.
7. multichannel coherent Raman scattering Optical devices according to claim 6, which is characterized in that the first optics group
Part further includes the first fiber amplifier and the first beam collimator；Wherein,
First fiber amplifier and the first beam collimator be sequentially arranged in first narrow band filter and times frequency module it
Between；
The seed light of first fiber amplifier, the assigned frequency for filtering out first narrow band filter carries out
Amplification, and export to first beam collimator；
First beam collimator for collimating the amplified seed light, and is exported to described times of frequency module.
8. multichannel coherent Raman scattering Optical devices according to claim 1, which is characterized in that the second optics group
Part successively includes the second narrow band filter, the second fiber amplifier, the second beam collimator and light path adjustment mechanism；Wherein,
Second narrow band filter, it is specified for being filtered out from the beam splitter beam splitting into the seed light of pumping branch road
The stokes light of frequency, and export to second fiber amplifier；
Second fiber amplifier for amplifying the stokes light, and is exported to second beam collimation
Device；
Second beam collimator for collimating the stokes light of amplification, and is exported to the light path tune
Complete machine structure；
The light path adjustment mechanism, the light path for the stokes light to the collimation are adjusted, so that each institute
The light path for stating the stokes light of Stokes branch is consistent with the pumping light path of pump light of branch road.
9. a kind of multichannel coherent Raman scattering micro imaging system, which is characterized in that including be sequentially arranged optical fiber laser,
Optoisolator, multichannel coherent Raman scattering Optical devices as claimed in any one of claims 1 to 8, laser galvanometer, microscope
With optical signal detection equipment；Wherein,
The optical fiber laser, for seed light to be input to the Optical devices by optoisolator；
The multichannel coherent Raman scattering Optical devices, are used to generate coherent Raman scattering for converting the seed light to
The pump light of signal and at least two beam stokes lights, and close beam and export to the laser galvanometer；
The laser galvanometer, for the pump light and stokes light that close beam to be input in the microscope, so that
Coherent Raman scattering signal is generated at the microscopical sample；
The optical signal detection equipment, for detecting the coherent Raman scattering signal and being carried out to the coherent Raman scattering signal
Imaging.
10. multichannel coherent Raman scattering micro imaging system according to claim 9, which is characterized in that the light letter
Number detecting devices successively includes optical filter, photodetector and signal processor；Wherein,
The optical filter for filtering out the veiling glare of the coherent Raman scattering signal, and will filter out the phase dry drawing after veiling glare
Graceful scattered signal is exported to the photodetector；
The photodetector for converting corresponding electric signal for the coherent Raman scattering signal, and is exported to described
Signal processor；
The signal processor, for the electric signal to be carried out imaging.
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Application publication date: 20181207 |