CN108823109A - A kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application - Google Patents
A kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application Download PDFInfo
- Publication number
- CN108823109A CN108823109A CN201810821056.1A CN201810821056A CN108823109A CN 108823109 A CN108823109 A CN 108823109A CN 201810821056 A CN201810821056 A CN 201810821056A CN 108823109 A CN108823109 A CN 108823109A
- Authority
- CN
- China
- Prior art keywords
- mortierella
- trichoderma strain
- mortierella alpine
- alpine trichoderma
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
Abstract
This application discloses a kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its applications, its classification naming is Mortierella alpina (Mortierellaalpina) GM712, it has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number CGMCCNo.15272.Fermenting and producing arachidonic acid is carried out using the bacterial strain, yield reaches as high as 14.0g/L, and yield is up to 1.76g/L/d.The bacterial strain can be used for industrialized production arachidonic acid.
Description
Technical field
The present invention relates to Mortierella alpine trichoderma strain more particularly to a kind of Mortierella alpine moulds of high-yield peanut tetraene acid lipid
Strain and its application.
Background technique
Arachidonic acid (ArachidonicAcid, abbreviation ARA, i.e. Arachidonic Acid) belongs to ω -6
Serial long-chain polyunsaturated fatty acid.ARA is a kind of important essential fatty acid, is many 20 carbonic acid derivatives as before
Column parathyrine E2 (PGE2), prostacyclin (PGI2), thromboxane A2 (TXA2), Leukotirene B4 (LTB4) and C4
(LTC4) direct precursor, the metabolism of these biological active matter confrontation lipoprotein, hemorheology, blood vessel elasticity, leucocyte function
Energy and platelet activation etc. have important adjustment effect.ARA, which also has, adjusts internal different kinds of ions channeling, maintains cell
Film function controls permeability of cell membranes, maintains many important physiological functions such as body water-retaining property.Meanwhile ARA is to constitute mind
Important feature lipid through organizing, it is most important to the brain growth of infant and visual function.It can help nervous system
Information is normally transmitted, and enhancing memory plays particularly important effect to human body eyesight, cerebral function and the coordination ability.In view of ARA
To the important function of infant's growth, the World Health Organization (WHO) and FAO (Food and Agriculture Organization of the United Nation) (FAO) authority recommend：Infant
40 milligrams of ARA (with the calculating of every kg body weight) should be absorbed daily, and the baby for only adding ARA has been strictly required in Europe and the U.S.
It sells in youngster's milk powder market of being allowed for access.
ARA is widely present in animal body, and past business ARA is mainly derived from pork liver and fish oil, but content is extremely low and not
Stablize, development cost valuableness, it is difficult to which ARA amount needed for meeting the industries such as the needs of social, especially dairy produce is very big.Due to micro-
Biology has many advantages, such as that the fast speed of growth, grease and ARA content are high, fermentation preparation process is fairly simple and is not limited by raw material,
Become the hot spot of various countries' research using microbial method production arachidonic acid.And screening the microbial resources for obtaining being rich in ARA is
It is crucial.
Summary of the invention
The purpose of the invention is to provide a kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application,
It solves one or more of the above-mentioned prior art problem.
According to an aspect of the present invention, a kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid is provided, point
Class is named as Mortierella alpina (Mortierella alpina) GM712, has been preserved in Chinese microorganism strain preservation management committee
Member's meeting common micro-organisms center (CGMCC), deposit number are CGMCC No.15272。
In some embodiments：The available carbon source of Mortierella alpine trichoderma strain be glucose, fructose, maltose, sucrose,
Lactose, D- galactolipin, glycerol, soluble starch or potato starch.
In some embodiments：The available nitrogen source of Mortierella alpine trichoderma strain is inorganic ammonium salt, mineral sulfates, inorganic
Nitrate, yeast extract, yeast extract, peptone, groundnut meal or beancake powder.
Application of the Mortierella alpine trichoderma strain in production arachidonic acid.
The mould of high-yield peanut tetraenoic acid provided by the invention, bacterial strain code name are GM712.In MEA culture medium, 25 DEG C of cultures
7d, bacteria colony white, diameter 50-55mm, quality velvet shape, reverse side is light yellow, and no diffusate generates, and no soluble pigment generates；
Mycelia winds branch, diameter 1.8-3um, no conidium structure；Mycelia winding generates a large amount of nest shape zoogamy structures, closely
Spherical shape, diameter 150-300um.It is identified as Mortierella alpina (Mortierella alpina).
The bacterial strain can be grown at 0~38 DEG C, and 27~29 DEG C are optimum growth temperature, but the optimum temperature for producing ARA is 28
℃.At 28 DEG C, the ARA that 6~7 days bacterial strains of shake flask fermentation generate is up to maximum value.
GM712 bacterial strain can be grown in the range of pH3~8 of culture solution, and generate arachidonic acid.Suitable growth and life
PH at ARA is 5~7, and optimum growh and the pH for generating ARA are 6.5.
GM712 bacterial strain is suitble to liquid growth, can be expanded by fermentor and be cultivated, had and occupy little space, production scale
The features such as being relatively easy to control is conducive to be mass produced.GM712 bacterial strain vitality is strong, simple to nutritional requirement, and growth scope is wide,
The bacterium oil ARA content of production is high, and is easy to extract, and has good development prospect.
Beneficial effects of the present invention：
It is CGMCC No. the present invention provides a kind of deposit number of high-yield peanut tetraene acid lipid (ARA)15272Height
Mountain Mortierella bacterial strain carries out shake flask fermentation using the bacterial strain, and ARA yield reaches as high as 14.0g/L, and yield is up to 1.76g/L/d,
Arachidonic acid accounts for fat content up to 60% or more in mycelia, less side products.It is 28 DEG C that arachidonic acid, which most preferably accumulates temperature,
Fermentation process is easier to control.
The acquisition of Mortierella alpina GM712 bacterial strain is not only that ARA production in China increases a new varieties, it is often more important that should
Strain can by liquid fermentation realize industrialized production, therefore, the invention will the ARA industry to China generate positive promotion
Effect.
Preservation information：
Mortierella alpina Mortierella alpina GM712 of the invention is on January 15th, 2018 in the micro- life of China
Object culture presevation administration committee common micro-organisms center (CGMCC) has carried out preservation, and collection address is：Beijing, China
Institute of microbiology of the academy of sciences, postcode：100101.Deposit number is：CGMCC No.15272。
Detailed description of the invention
Fig. 1 is the macroscopic form figure of Mortierella alpine trichoderma strain of the present invention；
Fig. 2 is the micromorphology figure of Mortierella alpine trichoderma strain of the present invention.
Specific embodiment
Illustrate with reference to the accompanying drawing, invention is further described in detail.
Wherein, Fig. 1 is the macroscopic form figure of Mortierella alpine trichoderma strain；Fig. 2 is the micromorphology figure of Mortierella alpine trichoderma strain.
Embodiment 1：
Mortierella alpina Mortierella alpina GM712 fatty acid compositional analysis
Fermentation strain is Mortierella alpina Mortierella alpina GM712, carries out liquid state fermentation, wherein fermentation training
Support base composition (w/v)：Glucose 50, yeast powder 20, sodium glutamate 4, potassium dihydrogen phosphate 1.5, pH7.0 ± 0.2.Fermentation process stream
Add the glucose solution for mending 600g/L concentration, control fermentation liquid sugar concentration 10-15g/L, the temperature of fermentation is 28 DEG C, fermentation time
It is 7 days, fermentation ends.Mycelium is filtered and collects, and freeze-drying is constant to weight, mycelium is ground by mortar.
The mycelium for taking 0.1g to dry, has been added in lid test tube.The hydrogen-oxygen that 5mL concentration is 0.4mol/L is added thereto
Change potassium-methanol solution, keep 60min in 50 DEG C of water-baths, then it is 14% boron trifluoride-methanol that the concentration of 5mL is added thereto
Solution keeps 60min in 50 DEG C of water-baths, and the extracting of 5mL n-hexane is added, and is eventually adding the washing of 4mL saturated sodium chloride solution, moves
Take upper solution using GC-MS combination detection.
As shown in table 1 below, analysis is the result shows that in the grease that above-mentioned bacterial strains generate based on arachidonic acid, and peanut four
Olefin(e) acid content is up to 60% or more.
Chromatographic condition：Finnigan Trace MS (U.S.) gas chromatograph-mass spectrometer.Using DB-MAX capillary column
(30m×0.25mm×0.25μm).185 DEG C of column temperature of starting keeps 1min, is increased to 225 DEG C with 5 DEG C/min, keeps 10min.
260 DEG C of injector temperature, carrier gas is helium, and flow velocity 1.0mL/min, split ratio is 10 ︰ 1.
Mass Spectrometer Method condition：EI+ (70Ev), emission current 200 μ A, 350 DEG C of ion source temperature, 260 DEG C of interface temperature.
1 fatty acid of table composition
| Title | C16:0 | C18:0 | C18:1 | C18:2 | C18:3 | C20:3 | C20:4 | It is other |
| Account for total fatty acids ratio (%) | 5.1 | 8.3 | 9.7 | 9.1 | 3.7 | 3.8 | 60.7 | 0.6 |
Embodiment 2：
The determination of best incubation time
The Mortierella alpina Mortierella alpina GM712 strain inoculated being stored on inclined-plane to solid PDA is trained
It supports in base, 30 DEG C of activation, with aseptic water washing inclined-plane, are made 10 until spore maturation8The spore suspension of a/mL or more, with 2%
Inoculum concentration is inoculated into liquid seed culture medium, and 28 DEG C are cultivated 1 day.It is inoculated into fermentation medium with 10% inoculum concentration, 28 DEG C
Culture 4-8 days.
Using the best incubation time of single factor exploration arachidonic acid accumulation, respectively on day 4, the 5th day, the 6th day, the
Sampling in 7 days and the 8th day is primary, measures dry weight and arachidonic acid yield.Fermentation medium forms (w/v)：Glucose 80, sulfuric acid
Ammonium 5, magnesium sulfate (MgSO4·7H2O) 0.5, potassium dihydrogen phosphate 1.5,6.5,115 DEG C of sterilizing 20min of pH.Bacterium as can be seen from Table 5
The best incubation time of strain fermentation producing arachidonic acid is the 7th day.
The arachidonic acid condition of production under the different incubation times of table 2
| Fermentation time | 4th day | 5th day | 6th day | 7th day | 8th day |
| Dry cell weight g/L | 29.0 | 32.8 | 35.4 | 39.1 | 39.0 |
| Arachidonic acid yield g/L | 8.81 | 10.84 | 12.02 | 13.27 | 13.02 |
Embodiment 3：
The determination of optimum carbon source
The Mortierella alpina Mortierella alpina GM712 strain inoculated being stored on inclined-plane to solid PDA is trained
It supports in base, 30 DEG C of activation, with aseptic water washing inclined-plane, are made 10 until spore maturation8The spore suspension of a/mL or more, with 2%
Inoculum concentration is inoculated into liquid seed culture medium, and 28 DEG C are cultivated 1 day.It is inoculated into fermentation medium with 10% inoculum concentration, 28 DEG C
Culture 7 days.
Using the optimum carbon source of single factor exploration arachidonic acid accumulation, respectively using fructose, maltose, sucrose, lactose,
D- galactolipin, glycerol, soluble starch or potato starch substitute the glucose in basic fermentation medium and carry out liquid state fermentation,
Measure dry weight and arachidonic acid yield.Liquid seed culture medium and basic fermentation medium composition are (w/v, similarly hereinafter)：Portugal
Grape sugar 80, ammonium sulfate 5, magnesium sulfate (MgSO4·7H2O) 0.5, potassium dihydrogen phosphate 1.5,6.5,115 DEG C of sterilizing 20min of pH.From table
2 it can be seen that the optimum carbon source of strain fermentation producing arachidonic acid is glucose.
The arachidonic acid condition of production under 3 different carbon source of table
Embodiment 4：
The determination of optimum nitrogen source
The Mortierella alpina Mortierella alpina GM712 strain inoculated being stored on inclined-plane to solid PDA is trained
It supports in base, 30 DEG C of activation, with aseptic water washing inclined-plane, are made 10 until spore maturation8The spore suspension of a/mL or more, with 2%
Inoculum concentration is inoculated into liquid seed culture medium, and 28 DEG C are cultivated 1 day.It is inoculated into fermentation medium with 10% inoculum concentration, 28 DEG C
Culture 7 days.
Using the optimum nitrogen source of single factor exploration arachidonic acid accumulation, respectively using ammonium nitrate, yeast powder, yeast extract,
Peptone, groundnut meal or beancake powder substitute the ammonium sulfate in basic fermentation medium and carry out liquid state fermentation, measure dry weight and flower
Raw tetraene acid yield.Basic fermentation medium composition (w/v)：Glucose 80, ammonium sulfate 5, magnesium sulfate (MgSO4·7H2O) 0.5,
Potassium dihydrogen phosphate 1.5,6.5,115 DEG C of sterilizing 20min of pH.The best nitrogen of strain fermentation producing arachidonic acid as can be seen from Table 3
Source is yeast powder.
The arachidonic acid condition of production under 4 different nitrogen sources of table
Embodiment 5：
The determination of optimum temperature
The Mortierella alpina Mortierella alpina GM712 strain inoculated being stored on inclined-plane to solid PDA is trained
It supports in base, 30 DEG C of activation, with aseptic water washing inclined-plane, are made 10 until spore maturation8The spore suspension of a/mL or more, with 2%
Inoculum concentration is inoculated into liquid seed culture medium, and 28 DEG C are cultivated 1 day.It is inoculated into fermentation medium with 10% inoculum concentration, culture 7
It.
Using the optimum temperature of single factor exploration arachidonic acid accumulation, 19 DEG C, 22 DEG C, 25 are set by fermentation temperature
DEG C, 28 DEG C, 31 DEG C of five gradients, measure dry weight and arachidonic acid yield.Fermentation medium forms (w/v)：Glucose 80, sulphur
Sour ammonium 5, magnesium sulfate (MgSO4·7H2O) 0.5, potassium dihydrogen phosphate 1.5,6.5,115 DEG C of sterilizing 20min of pH.As can be seen from Table 4
The optimum temperature of strain fermentation producing arachidonic acid is 28 DEG C.
The arachidonic acid condition of production under 5 different temperatures of table
Embodiment 6：
The determination of Optimal pH
The Mortierella alpina Mortierella alpina GM712 strain inoculated being stored on inclined-plane to solid PDA is trained
It supports in base, 30 DEG C of activation, with aseptic water washing inclined-plane, are made 10 until spore maturation8The spore suspension of a/mL or more, with 2%
Inoculum concentration is inoculated into liquid seed culture medium, and 28 DEG C are cultivated 1 day.It is inoculated into fermentation medium with 10% inoculum concentration, 28 DEG C
Culture 7 days.
Using the Optimal pH of single factor exploration arachidonic acid accumulation, will fermentation pH value be set as 4.5,5.0,5.5,6.0,
6.5,7.0,7.5 7 gradients measure dry weight and arachidonic acid yield.Fermentation medium forms (w/v)：Glucose 80, sulphur
Sour ammonium 5, magnesium sulfate (MgSO4·7H2O) 0.5,1.5,115 DEG C of sterilizing 20min of potassium dihydrogen phosphate.Bacterial strain is sent out as can be seen from Table 5
The Optimal pH of ferment producing arachidonic acid is 6.5.
The arachidonic acid condition of production under the different pH value of table 6
The above is only one embodiment of the present invention, it is noted that those skilled in the art,
Without departing from the concept of the premise of the invention, some similar deformation and improvement can be made, these also should be regarded as this
Within the protection scope of invention.
Claims (4)
1. a kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid, classification naming is Mortierella alpina
(Mortierella alpina) GM712, has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center
(CGMCC), deposit number is CGMCC No.15272。
2. a kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid according to claim 1, it is characterised in that：Institute
State the available carbon source of Mortierella alpine trichoderma strain be glucose, it is fructose, maltose, sucrose, lactose, D- galactolipin, glycerol, solvable
Property starch or potato starch.
3. a kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid according to claim 1, it is characterised in that：Institute
State the available nitrogen source of Mortierella alpine trichoderma strain be inorganic ammonium salt, mineral sulfates, inorganic nitrate, yeast extract, yeast extract,
Peptone, groundnut meal or beancake powder.
4. application of the Mortierella alpine trichoderma strain described in claim 1 in production arachidonic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810821056.1A CN108823109A (en) | 2018-07-24 | 2018-07-24 | A kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810821056.1A CN108823109A (en) | 2018-07-24 | 2018-07-24 | A kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108823109A true CN108823109A (en) | 2018-11-16 |
Family
ID=64140547
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810821056.1A Pending CN108823109A (en) | 2018-07-24 | 2018-07-24 | A kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108823109A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110819539A (en) * | 2019-11-27 | 2020-02-21 | 河南农业大学 | Mortierella alpina 0901, application thereof and microbial inoculum |
| CN112625912A (en) * | 2020-09-09 | 2021-04-09 | 中国科学院微生物研究所 | Mortierella alpina strain XY05201 and application thereof |
| WO2021104164A1 (en) * | 2019-11-26 | 2021-06-03 | 瞿瀚鹏 | Mortierella alpina and use thereof, and microbial oil rich in ara at position sn-2, preparation method therefor and use thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703332A (en) * | 2012-06-19 | 2012-10-03 | 南京工业大学 | Bacterial strain for producing arachidonic acid oil and application thereof |
| CN102827780A (en) * | 2012-09-25 | 2012-12-19 | 江南大学 | Mortierella alpine strain for producing arachidonic acid |
| CN103805517A (en) * | 2012-11-14 | 2014-05-21 | 丰益(上海)生物技术研发中心有限公司 | New separated bacterial strain of mortierella alpine and application thereof |
| RU2013133213A (en) * | 2013-07-16 | 2015-01-27 | Общество с ограниченной ответственностью "Органик парк" | MUSHROOM STRAIN Mortierella alpina F-1134 FOR OBTAINING A HIGHLY ACTIVE PLANT GROWTH REGULATOR |
| US20150252395A1 (en) * | 2012-09-14 | 2015-09-10 | Roquette Freres | Oil enriched with arachidonic acid of microorganisms (unicellular fungus mortierella alpina) and method for the production thereof |
| CN106244468A (en) * | 2016-09-23 | 2016-12-21 | 厦门大学 | A kind of regulate and control Mortierella alpina fermentation producing arachidonic acid preparation method |
| CN110964761A (en) * | 2019-12-13 | 2020-04-07 | 江苏远大仙乐药业有限公司 | Tremella and application thereof |
-
2018
- 2018-07-24 CN CN201810821056.1A patent/CN108823109A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703332A (en) * | 2012-06-19 | 2012-10-03 | 南京工业大学 | Bacterial strain for producing arachidonic acid oil and application thereof |
| US20150252395A1 (en) * | 2012-09-14 | 2015-09-10 | Roquette Freres | Oil enriched with arachidonic acid of microorganisms (unicellular fungus mortierella alpina) and method for the production thereof |
| CN102827780A (en) * | 2012-09-25 | 2012-12-19 | 江南大学 | Mortierella alpine strain for producing arachidonic acid |
| CN103805517A (en) * | 2012-11-14 | 2014-05-21 | 丰益(上海)生物技术研发中心有限公司 | New separated bacterial strain of mortierella alpine and application thereof |
| RU2013133213A (en) * | 2013-07-16 | 2015-01-27 | Общество с ограниченной ответственностью "Органик парк" | MUSHROOM STRAIN Mortierella alpina F-1134 FOR OBTAINING A HIGHLY ACTIVE PLANT GROWTH REGULATOR |
| CN106244468A (en) * | 2016-09-23 | 2016-12-21 | 厦门大学 | A kind of regulate and control Mortierella alpina fermentation producing arachidonic acid preparation method |
| CN110964761A (en) * | 2019-12-13 | 2020-04-07 | 江苏远大仙乐药业有限公司 | Tremella and application thereof |
Non-Patent Citations (6)
| Title |
|---|
| HIROSHI KIKUKAWA等: "Arachidonic acid production by the oleaginous fungus Mortierella alpina 1S-4: A review", 《JOURNAL OF ADVANCED RESEARCH》 * |
| TOMOYO OKUDA等: "Omega-3 eicosatetraenoic acid production by molecular breeding of the mutant strain S14 derived from Mortierella alpina 1S-4", 《JOURNAL OF BIOSCIENCE AND BIOENGINEERING》 * |
| 刘晓婷等: "一株新型花生四烯酸生产菌株高山被孢霉LU166的形态调控及维生素培养优化", 《厦门大学学报（自然科学版）》 * |
| 张莉等: "高山被孢霉产花生四烯酸研究概况 ", 《北方药学》 * |
| 徐锐等: "《发酵技术》", 31 January 2016 * |
| 顾晓颖等: "高产花生四烯酸的高山被孢霉菌株发酵条件优化", 《西南农业学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021104164A1 (en) * | 2019-11-26 | 2021-06-03 | 瞿瀚鹏 | Mortierella alpina and use thereof, and microbial oil rich in ara at position sn-2, preparation method therefor and use thereof |
| CN110819539A (en) * | 2019-11-27 | 2020-02-21 | 河南农业大学 | Mortierella alpina 0901, application thereof and microbial inoculum |
| CN110819539B (en) * | 2019-11-27 | 2021-05-07 | 河南农业大学 | Mortierella alpina 0901, application thereof and microbial inoculum |
| CN112625912A (en) * | 2020-09-09 | 2021-04-09 | 中国科学院微生物研究所 | Mortierella alpina strain XY05201 and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103146584B (en) | Liquid fermentation production method of docosahexenoic acid (DHA) through solid material cultivation of schizochytrium sp. | |
| CN102864111B (en) | Schizochytrium limacinum strain for producing docosahexaenoic acid | |
| CN102888348A (en) | Schizochytrium limacinum and method or fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of schizochytrium limacinum | |
| CN102925504B (en) | Method and fermentation culture medium used for synthesizing gamma-aminobutyric acid through microbial fermentation | |
| CN108823109A (en) | A kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application | |
| CN108034599B (en) | One plant of Lactobacillus brevis for efficiently synthesizing γ-aminobutyric acid from brewed spirit system | |
| CN102268377A (en) | Method for improving lipid producing microalga biomass and lipid accumulation with two stage culture strategy of mixotrophic and nitrogen-rich-nitrogen-deficient culture | |
| CN1986822A (en) | Crypthecodinium connii fermenting process for producing docosahexaenoic acid grease | |
| CN107937311B (en) | Streptococcus thermophilus for high yield of gamma-aminobutyric acid, preservation and culture method and method for preparing fermented milk by using streptococcus thermophilus | |
| CN108004149A (en) | A kind of ocean protist and the method using its fermenting and producing high added value lipid product | |
| KR20220088912A (en) | Schizochytrium and its application, Sn-2 DHA-rich microorganism maintenance and its manufacturing method and application | |
| CN101255450B (en) | Technique for producing L-malic acid by using rhizopus oryzae fermentation | |
| CN105420122A (en) | Schizochytrium limacinum suitable for high-density culture and method for producing grease rich in DHA | |
| CN101041837B (en) | Preparation method of new natural abscisic acid | |
| CN104087512A (en) | Mortierella alpine producing polyunsaturated fatty acids and application thereof | |
| CN102674929A (en) | Inonotus obliquus submerged fermentation culture medium and submerged fermentation method thereof | |
| CN107460215A (en) | A kind of method of microalgae mixed culture production grease | |
| CN101113410A (en) | Mortierella alpina and uses thereof | |
| CN106190872B (en) | Fermentor bottoms stream adds the method for production docosahexaenoic acid | |
| CN107488615A (en) | The pseudomonad of one plant height yielding lipase and its enzymatic production method | |
| CN102634460A (en) | Rhizopus oryzae RH1-5 and separating and culturing method thereof | |
| CN104087632B (en) | A kind of deep layer liquid fermentation produces the method for Phellinus igniarius (L. ex Fr.) Quel. extracellular polysaccharide | |
| CN103981106B (en) | DHA superior strain and application thereof | |
| CN106244468A (en) | A kind of regulate and control Mortierella alpina fermentation producing arachidonic acid preparation method | |
| CN106010970B (en) | A kind of hidden dinoflagellate of Kou Shi and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181116 |
|
| RJ01 | Rejection of invention patent application after publication |