CN108782045A - A kind of sealwort cultivating and growing method - Google Patents
A kind of sealwort cultivating and growing method Download PDFInfo
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- CN108782045A CN108782045A CN201810554205.2A CN201810554205A CN108782045A CN 108782045 A CN108782045 A CN 108782045A CN 201810554205 A CN201810554205 A CN 201810554205A CN 108782045 A CN108782045 A CN 108782045A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of sealwort cultivating and growing methods quickly bred that can realize sealwort.The sealwort cultivating and growing method includes reserving seed for planting, selection of land, site preparation, sealwort seed seedling-raising, field management, pest control, by carrying out Fiber differentiation to sealwort seed, squamous subculture, differentiation culture, and it is directed to the distinctive hibernation feature of sealwort seed, improvement design is carried out to inducing culture, the hibernation feature of sealwort seed can be abolished in Induction Process, it can fast germination, and improve germination percentage, the survival rate of sealwort seedling can be greatly improved by hardening treatment, in seedling root, sealwort growth retardation under drought stress can be effectively relieved in the outer source additive of application simultaneously, blade is wilted, the drought resistance of sealwort can significantly be improved, overcome the season limit of explant supply, the method for culturing seedlings only needs three months or so simultaneously, it can realize the quick breeding of sealwort.It is suitble to promote the use in biotechnology.
Description
Technical field
The invention belongs to biotechnologies, and in particular to a kind of sealwort cultivating and growing method.
Background technology
Sealwort is sweet in flavor, mild-natured.Returns spleen, lung, kidney channel.With boosting qi and nourishing yin, invigorating the spleen, moistening lung, kidney benefiting functions.For taste void
It is weak, physically weak weak, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, Heat Diabetes.Modern study, to diabetes, high blood pressure, the heart
The diseases such as vascular diseases, tuberculosis, chronic hepatitis, and have preferable effect in antibacterial, removing toxic substances, antifatigue, anti-aging etc..
Its chemical composition mainly have Siberian solomonseal rhizome polysaccharide, steroid saponin, anthraquinone analog compound, alkaloid, cardiac glycoside, lignanoid, vitamin and
A variety of amino acid etc. useful to human body.
As long as the cultivating and growing of sealwort depends on nursery and later stage field management, sealwort nursery is mainly sealwort seed seedling-raising
With two kinds of root-like stock nursery.Since stem tuber breeding belongs to vegetative propagation, easy to carry virus, variety deterioration cause sealwort yield and
Quality reduces, and sealwort seed has physiology after-ripening and hibernation feature, seed sprouting difficult, and present sealwort seed seedling-raising technology is immature
And the seed seedling-raising period is long, it usually needs just understands germinating to seedling within 3 years or so, and germination percentage is relatively low.As medicinal material market is to sealwort
Demand increases, and sealwort has quickly been multiplied into problem urgently to be resolved hurrily, causes the yield of sealwort not high, and cannot be satisfied market needs
It asks.
Invention content
Technical problem to be solved by the invention is to provide a kind of sealwort cultigens quickly bred that can realize sealwort
Plant method.
The technical solution adopted by the present invention to solve the technical problems is：The sealwort cultivating and growing method, including following step
Suddenly：
A, it reserves seed for planting；Sealwort seed carries out Sand culture immediately in annual August maturation, the seed for choosing mature and plump：Seed 1
Part, 3 parts of sand is uniformly mixed, and is stored in the hole of 30 centimetres of depths of shady spot, and moistening is kept；
B, selection of land, site preparation；Selection moistens and has the plot fully covered, and soil is loose with quality, the good loam of moisture holding capacity
Or sandy loam is advisable, and is first ploughed deeply 1 time before sowing, applies 2000 kilograms of farm manure per acre in conjunction with site preparation, plough under and make base manure in soil, then
The thin leveling of rake, bedding, furrow are 1.2 meters wide.
C, sealwort seed seedling-raising：
C1, seed selection：Sealwort seed is chosen, and is carried out disinfection processing to sealwort seed；
C2, the sealwort seed after washing and sterilizing is inoculated into inducing culture and cultivates 26~30d in an aseptic environment and obtains
To callus, the inducing culture by N6 culture mediums, GA3,100mg/L of 150mg/L IAA, 300mg/LETH,
The SNP of 500mg/L, the 2 of Vb stock, the sucrose of 30g/L, 5.0mg/L, the hydrogen peroxide of 4-D, 100mg/L, the agar of 4.5g/L
Composition, the PH=5.8 of the inducing culture；
C3, callus is transferred in subculture medium and cultivates 5~9d in an aseptic environment, the subculture medium is by MS
The 2 of culture medium, the sucrose of 30g/L, 5.0mg/L, the work of the potassium permanganate, 1.0g/L of 6-BA, 100mg/L of 4-D, 100mg/L
Property charcoal, 4.5g/L agar composition, the PH=5.8 of the subculture medium；
C4, will be transferred in differential medium by the callus of step C3 processing cultivate 18 in an aseptic environment~
22d, the differential medium by MS culture mediums, the sucrose of 30g/L, 0.2mg/L NAA, 50mg/L GA3,1g/L sulfuric acid
Copper, 100mg/L 6-BA, 4.5g/L agar composition, the PH=5.8 of the differential medium；
C5, it will be transferred in tissue culture bottle by the callus of step C4 processing, and tissue culture bottle be placed on intensity of illumination
For 1500~3000lx, 5~8d is cultivated in the gnotobasis that temperature is 25~30 DEG C, light application time is 38~42h/d；
C6, tissue culture bottle is placed on to temperature it is 20~30 DEG C and have 4~7d of hardening culture in collarium border, in hardening culture
Cheng Zhong applies outer source additive in seedling root, and the outer source additive includes solvent and solute, and the solvent is Gram suddenly
Nutrient solution, the solute include chitosan (CTS) and spermine (Spm), and a concentration of 0.5g/L-1.5g/L of the CTS is described
A concentration of 50.58mg/L-151.74mg/L of Spm；
C7, when seedling grows to 2~3cm, be transplanted in whole good field；
D, field management：Early period is wanted frequent tillage and weeding in sealwort growth, is respectively carried out 1 time 4,6,9, November every year, preferably shallow
It uproots and suitably earths up, later stage uprooting weed；Top dressing is carried out during tillage and weeding, applies farmyard manure after 3 times intertilling per acre
1500 kilograms, and 50 kilograms of calcium superphosphate, 50 kilograms of cake fertilizer, mixing mix thoroughly after after ditching-fertilizing in the ranks, fertilising earthing lid fertilizer；
It needs to irrigate in time in sealwort growth course；Keep ground moistening；
E, pest control；Deinsectization is carried out using physical deinsectization method.
Further, in step D, irrigating sealwort and using following irrigation system：The irrigation system includes multiple
Plant module, water tank and the irrigation conduit being connected to the water tank；The irrigation conduit includes spray header and sprinkling irrigation
Water pipe；Multiple spray heads are communicated on the spray header, the spray head is located at the top of multiple plantation modules；It is described
Sprinkling irrigation water pipe is communicated with multiple rainers in earth's surface；The rainer includes accommodating pipe, multiple soakaway trench, multidirectional
Shower head and jacking cylinder；The Multi-direction sprinkling irrigation head is connected with the sprinkling irrigation water pipe；The receiving pipe and multiple infiltrations
Guan Jun is embedded under earth's surface；The Multi-direction sprinkling irrigation head is arranged in the receiving pipe, and can be under the driving of the jacking cylinder
Stretch out or take in the receiving pipe；The soakaway trench is connected with the receiving pipe, and accommodates radially outward prolonging for pipe along described
It stretches；Multiple water seepage holes are offered on the soakaway trench；The bottom for accommodating pipe offers through-hole, the jacking of the jacking cylinder
Bar passes through the through-hole to be connected to the bottom of the Multi-direction sprinkling irrigation head, further includes sun-shading mechanism；The sun-shading mechanism includes sunshade
Cloth, frame and driving motor；The puggaree is fixed in the frame, and driving is arranged in the drive shaft of the driving motor
Gear offers driven tooth corresponding with the driving gear on the frame, and the driven tooth is mutually nibbled with the driving gear
It closes, multiple loopholes identical with spray head size, the position of the loophole and the spray is offered on the puggaree
The position of head is corresponding.
Further, the planting rhizoma polygonati irrigation system further include multiple control valves, controller, illuminance sensor and
Humidity sensor for detecting surface humidity；Multiple control valves are correspondingly arranged at the spray header and the spray respectively
On irrigation pipe；The controller is electrically connected with the control valve, the intensity of illumination sensor and the humidity sensor respectively；
The controller adjusts the position of the puggaree according to the induction of the intensity of illumination sensor；The controller is according to
The induction of humidity sensor controls the open and close of the control valve and the sprinkling irrigation mode of the rainer.
Further, the soakaway trench and the lifting pin directly pass through seal with elastometic washer, the length of the soakaway trench
For 400-800mm；A diameter of 3-5mm of the water seepage hole, and the spacing between the adjacent water seepage hole is 10-20mm, it is described
The connectivity part for accommodating pipe and the soakaway trench is equipped with filter screen.
Further, in step E, the physical deinsectization method is realized using following delousing plant：The deinsectization dress
It sets including top deinsectization component and lower part deinsectization component；The top deinsectization component includes pedestal, cover plate, cover net, guiding net, draws
Worm lamp and heat conductive rod；The insect attractant light is fixed at the center of the pedestal, and the insect attractant light is internally provided with heat conductive rod；It is described
Cover plate is located at the online side of the cover, and the cover plate upper end is equipped with solar panel；Direct-flow storage battery is equipped in the pedestal；
The solar panel, the insect attractant light and the heat conductive rod are connected by conducting wire with the direct-flow storage battery respectively；It is described
The bottom of cover net is connected with the bottom of the guiding net, and worm inlet hole is offered at the top of the guiding net；The cover net and described
Pedestal is connected by multiple connecting poles；The lower part deinsectization component includes connected unit, guiding slope, collection assembly and volatilization room；Institute
It states connected unit and the pedestal is detachably connected；The guiding slope around the periphery for being located at the connected unit and with the connected unit one
It is body formed；The volatilization room is arranged inside the connected unit, and the volatilization interior is placed with attractant；It is opened in the connected unit
Equipped with multiple evaporation holes, multiple evaporation holes are connected with the volatilization room；The collection assembly is arranged on the guiding slope
With the inside of the connected unit, and multiple collection ports being connected to the collection assembly are offered on the guiding slope.
Further, the guiding net is in a circular table shape, reduced diameter from bottom to top, multiple connecting poles are along institute
The foreign aid for stating pedestal is uniformly distributed in week.
Further, being provided with lampshade outside the insect attractant light, the cover plate is in reverse V-shaped, and is set at the top of the cover plate
It is equipped with hanging ring.
Further, the collection assembly includes anti-ease bucket and collection box；The anti-ease bucket is arranged in the collection box
Between the collection port, and the anti-ease bucket is funnel-form.
Further, in step C1, to the processing mode of sealwort wash seeds sterilizing by the way of as described below：
First, sealwort seed is put into bottle, and addition soaked in absolute ethyl alcohol 1 minute in bottle, then sealwort seed taking-up is distilled
Water cleans up, and distilled water is then added in bottle and the sealwort seed cleaned up is put into bottle, bottle is placed in 40 DEG C
It carries out bubble kind in refrigerator to handle, the time of the bubble kind is 24-30h, after steeping kind, the taking-up of sealwort seed is put into a concentration of
Impregnate 30min in the salicylic acid solution of 13.8mg/Ld, after will orchardgrass mature seed take out after cleaned 3-5 times with distilled water, and
Sterilizing filter paper is placed it in, excessive moisture is dried.
Further, in step C2, the Vb stock that contain in the Fiber differentiation by 20mg/L Vb1,
The niacin of Vb6,9mg/L of 19.5mg/L form.
The beneficial effects of the present invention are：The sealwort cultivating and growing method include reserve seed for planting, selection of land, site preparation, sealwort seed are educated
Seedling, field management, pest control by carrying out Fiber differentiation, squamous subculture, differentiation culture to sealwort seed, and are directed to sealwort
The distinctive hibernation feature of seed carries out improvement design to inducing culture, sealwort seed can be abolished in Induction Process
Hibernation feature, can fast germination, and improve germination percentage, by hardening treatment can greatly improve sealwort seedling at
Motility rate, while sealwort growth retardation, blade are wilted in the case where drought stress can be effectively relieved in the seedling root outer source additive of application,
The drought resistance that sealwort can significantly be improved, avoid due to water shortage cause sealwort dry up the underproduction the case where occur, be selection and breeding sealwort
Improved seeds provide Technical Reference, while overcoming the season limit of explant supply as explant using sealwort seed,
The method for culturing seedlings only needs three months or so simultaneously, substantially reduces growing-seedling period, and germination percentage and survival rate are just higher,
It can realize the quick breeding of sealwort.
Description of the drawings
Fig. 1 is the structural schematic diagram of this irrigation system of the present invention；
Fig. 2 is the structural schematic diagram of the sun-shading mechanism of irrigation system of the present invention；
Fig. 3 is shown as the structural schematic diagram of heretofore described delousing plant.
Reference sign：1- pedestals, 2- cover plates, 3- cover nets, 4- guiding nets, 5- insect attractant lights, 6- heat conductive rods, 7- solar energy
Solar panel, 8- direct-flow storage batteries, 9- connecting poles, 10- connected units, 11- guiding slope, 12- collection assemblies, 13- volatilizations room, 14- draw
Lure, 100- kinds plant module, 200- water tanks, 300- spray headers, 400- sprinkling irrigation water pipes, 500- spray heads, 600- sprinkling irrigation dresses
It sets, 601- accommodates pipe, 602- soakaway trench, 603- shower heads, 604- jacking cylinders, 605- water seepage holes, 700- sun-shading mechanisms, 701-
Puggaree, 702- frames, 703- driving motors.
Specific implementation mode
Sealwort cultivating and growing method of the present invention, includes the following steps：
A, it reserves seed for planting；Sealwort seed carries out Sand culture immediately in annual August maturation, the seed for choosing mature and plump：Seed 1
Part, 3 parts of sand is uniformly mixed, and is stored in the hole of 30 centimetres of depths of shady spot, and moistening is kept；
B, selection of land, site preparation；Selection moistens and has the plot fully covered, and soil is loose with quality, the good loam of moisture holding capacity
Or sandy loam is advisable, and is first ploughed deeply 1 time before sowing, applies 2000 kilograms of farm manure per acre in conjunction with site preparation, plough under and make base manure in soil, then
The thin leveling of rake, bedding, furrow are 1.2 meters wide.
C, sealwort seed seedling-raising：
C1, seed selection：Sealwort seed is chosen, and is carried out disinfection processing to sealwort seed；
C2, the sealwort seed after washing and sterilizing is inoculated into inducing culture and cultivates 26~30d in an aseptic environment and obtains
To callus, the inducing culture by N6 culture mediums, GA3,100mg/L of 150mg/L IAA, 300mg/LETH,
The SNP of 500mg/L, the 2 of Vb stock, the sucrose of 30g/L, 5.0mg/L, the hydrogen peroxide of 4-D, 100mg/L, the agar of 4.5g/L
Composition, the PH=5.8 of the inducing culture；
C3, callus is transferred in subculture medium and cultivates 5~9d in an aseptic environment, the subculture medium is by MS
The 2 of culture medium, the sucrose of 30g/L, 5.0mg/L, the work of the potassium permanganate, 1.0g/L of 6-BA, 100mg/L of 4-D, 100mg/L
Property charcoal, 4.5g/L agar composition, the PH=5.8 of the subculture medium；Addition activated carbon not only effectively prevent browning, due to
The effect that hormonal milieu changes after addition 6-BA, the sensitivity that can also alleviate the growth of callus subculture to environment by adding activated carbon
Property, more callus is promoted to stablize growth；In addition, 2,4-D of low concentration and the 6-BA of higher concentration are applied in combination, both improve more
The quality of wound, and promote its differentiation, become callus and is finally divided into plant fabulous transition period；
C4, will be transferred in differential medium by the callus of step C3 processing cultivate 18 in an aseptic environment~
22d, the differential medium by MS culture mediums, the sucrose of 30g/L, 0.2mg/L NAA, 50mg/L GA3,1g/L sulfuric acid
Copper, 100mg/L 6-BA, 4.5g/L agar composition, the PH=5.8 of the differential medium；
C5, it will be transferred in tissue culture bottle by the callus of step C4 processing, and tissue culture bottle be placed on intensity of illumination
For 1500~3000lx, 5~8d is cultivated in the gnotobasis that temperature is 25~30 DEG C, light application time is 38~42h/d；
C6, tissue culture bottle is placed on to temperature it is 20~30 DEG C and have 4~7d of hardening culture in collarium border, in hardening culture
Cheng Zhong applies outer source additive in seedling root, and the outer source additive includes solvent and solute, and the solvent is Gram suddenly
Nutrient solution, the solute include chitosan (CTS) and spermine (Spm), and a concentration of 0.5g/L-1.5g/L of the CTS is described
A concentration of 50.58mg/L-151.74mg/L of Spm；The survival rate of sealwort seedling can be greatly improved by hardening treatment, simultaneously
Seedling root apply the outer source additive that above-mentioned drought resisting composition is formed can be effectively relieved under drought stress sealwort growth by
Resistance, blade wilt, can significantly improve the drought resistance of sealwort, avoid due to water shortage cause sealwort dry up the underproduction the case where send out
It is raw；
C7, when seedling grows to 2~3cm, transplanted.
D, field management：Early period is wanted frequent tillage and weeding in sealwort growth, is respectively carried out 1 time 4,6,9, November every year, preferably shallow
It uproots and suitably earths up, later stage uprooting weed；Top dressing is carried out during tillage and weeding, applies farmyard manure after 3 times intertilling per acre
1500 kilograms, and 50 kilograms of calcium superphosphate, 50 kilograms of cake fertilizer, mixing mix thoroughly after after ditching-fertilizing in the ranks, fertilising earthing lid fertilizer；
It needs to irrigate in time in sealwort growth course；Keep ground moistening；
E, pest control；Deinsectization is carried out using physical deinsectization method.
The sealwort cultivating and growing method include reserve seed for planting, selection of land, site preparation, sealwort seed seedling-raising, field management, pest control,
By carrying out Fiber differentiation, squamous subculture, differentiation culture to sealwort seed, and it is directed to the distinctive hibernation feature of sealwort seed, it is right
Inducing culture carries out improvement design, and the hibernation feature of sealwort seed can be abolished in Induction Process, can be fast
Speed germination, and germination percentage is improved, the survival rate of sealwort seedling can be greatly improved by hardening treatment, while applying in seedling root
Add outer source additive that sealwort growth retardation under drought stress, blade wilting can be effectively relieved, can significantly improve sealwort
Drought resistance, avoid due to water shortage cause sealwort dry up the underproduction the case where occur, for selection and breeding sealwort improved seeds provide technology join
It examines, while the season limit of explant supply is overcome, while the method for culturing seedlings only needs three as explant using sealwort seed
A month or so, growing-seedling period is substantially reduced, and germination percentage and survival rate are just higher, can realize the quick numerous of sealwort
It grows.
In step D, sealwort is irrigated and uses following irrigation system：As shown in Figure 1 to Figure 2, the irrigation system includes
Multiple plantation module 100, water tank 200 and the irrigation conduits being connected to water tank 200；Irrigation conduit includes spray header
300 and sprinkling irrigation water pipe 400；Multiple spray heads 500 are communicated on spray header 300, spray head 500 is located at multiple plantation modules
100 top；Sprinkling irrigation water pipe 400 is communicated with multiple rainers 600 in earth's surface；Rainer 600 includes accommodating to manage
601, multiple soakaway trench 602, Multi-direction sprinkling irrigation head 603 and jacking cylinder 604；Multi-direction sprinkling irrigation head 603 is connected with sprinkling irrigation water pipe 400
It is logical；It accommodates pipe 601 and multiple soakaway trench 602 is embedded under earth's surface；Multi-direction sprinkling irrigation head 603 is arranged in accommodating pipe 601, and can
It stretches out or takes under the driving of jacking cylinder 604 and accommodate pipe 601；Soakaway trench 602 is connected with pipe 601 is accommodated, and along receiving
Pipe 601 extends radially outwardly；Multiple water seepage holes 605 are offered on soakaway trench 602；The bottom for accommodating pipe 601 offers through-hole,
The lifting pin of jacking cylinder 604 passes through through-hole to be connected to the bottom of Multi-direction sprinkling irrigation head 603, further includes sun-shading mechanism 700；Sunshade machine
Structure 700 includes puggaree 701, frame 702 and driving motor 703；Puggaree 701 is fixed in frame 702, driving motor 703
Drive shaft on be arranged with driving gear, offered on frame 702 and the corresponding driven tooth of driving gear, driven tooth and sliding tooth
Wheel is meshed, and multiple loopholes identical with 500 size of spray head, the position of loophole and spray are offered on puggaree 701
First 500 position is corresponding.Such as intensity of illumination it is high when, sprinkling irrigation water pipe 400 may be used stretch out and accommodate pipe 601 and spray header
The 300 three-dimensional Irrigations worked at the same time, and when intensity of illumination is not high, pipe 601 is accommodated using the income of water pipe 400 that will spray,
Only carry out sub-irrigation.The position that loophole and spray head 500 are staggered is adjusted additionally by the position of adjustment puggaree 701
It sets, to adjust the intensity of illumination.By the loophole being uniformly arranged, the intensity of illumination entered an association can be made relatively uniform, met
The needs of sealwort growth.In conclusion above-mentioned irrigation system, can carry out ground spray and surface irrigation, moreover it is possible to as needed
Sub-irrigation is carried out, a system can realize a variety of irrigation methods, the production of enterprise is reduced while saving water resource
Cost；Solarization intensity can be additionally adjusted, ensures the abundant growth of sealwort.
Preferably, planting rhizoma polygonati irrigation system further includes multiple control valves, controller, illuminance sensor and for detecting
The humidity sensor of surface humidity；Multiple control valves are correspondingly arranged at respectively on spray header 300 and sprinkling irrigation water pipe 400；Control
Device is electrically connected with control valve, intensity of illumination sensor and humidity sensor respectively；Controller is according to the sense of intensity of illumination sensor
The position of puggaree 701 should be adjusted；Controller controls the open and close and spray of control valve according to the induction of humidity sensor
The sprinkling irrigation mode of filling device 600.Controller can be controlled according to different intensities of illumination and different surface humidities, adjustment
Different irrigation methods.
In the present invention, soakaway trench 602 and lifting pin directly pass through seal with elastometic washer.Preferably, pipe 601 and infiltration are accommodated
The connectivity part of pipe 602 is equipped with filter screen.Further, the length of soakaway trench 602 is 400-800mm；Water seepage hole 605 it is a diameter of
3-5mm, and the spacing between adjacent water seepage hole 605 is 10-20mm.
It is that the chemicals residual generated in order to avoid chemical deinsectization causes to endanger to sealwort using physical deinsectization in step E
Evil, while chemical residue can reduce the quality of sealwort, therefore the above problem, the object can thoroughly be prevented using physical deinsectization
Deinsectization method is managed to realize using following delousing plant：The delousing plant, as shown in figure 3, including top deinsectization component and lower part
Deinsectization component；Top deinsectization component includes pedestal 1, cover plate 2, cover net 3, guiding net 4, insect attractant light 5 and heat conductive rod 6；Insect attractant light 5 is solid
It is scheduled at the center of pedestal 1, insect attractant light 5 is internally provided with heat conductive rod 6；Cover plate 2 is located at 3 top of cover net, and 2 upper end of cover plate is equipped with
Solar panel 7；Direct-flow storage battery 8 is equipped in pedestal 1；Solar panel 7, insect attractant light 5 and heat conductive rod 6 are respectively by leading
Line is connected with direct-flow storage battery 8；The bottom of cover net 3 is connected with the bottom of guiding net 4, and the top of guiding net 4 offers worm inlet hole；
Cover net 3 is connected with pedestal 1 by multiple connecting poles 9；Lower part deinsectization component includes connected unit 10, guiding slope 11, collection assembly 12
With volatilization room 13；Connected unit 10 and pedestal 1 are detachably connected；Guide slope 11 around the periphery for being located at connected unit 10 and with connected unit 10
It is integrally formed；The setting of room 13 volatilize inside connected unit 10, attractant 14 is placed in room 13 of volatilizing；It is offered in connected unit 10
Multiple evaporation holes, multiple evaporation holes are connected with volatilization room 13；Collection assembly 12 is arranged in guiding slope 11 and connected unit 10
Portion, and guide and offer multiple collection ports being connected to collection assembly 12 on slope 11.In a preferred embodiment, 10 He of connected unit
Pedestal 1 is connected by the way of being threadedly coupled or snapping connection.In use, the pest of flight class is in insect attractant light 5 and heat conductive rod 6
Luring under fly into guiding net 4, since the setting of guiding net 4 can only be in guiding net 4 in conjunction with the habit of flight class pest
The lower flight upwards of guiding, enters cover net 3 after worm inlet hole, into cover net 3 after flight class pest will be trapped in nothing in cover net 3
Method flies out, and realizes that killing for flight class pest is removed.And the pest of reptiles under the luring of attractant 14 by guiding slope 11 to waving
Hair engaging aperture position is creeped, by being fallen into when collection port in collection assembly 12, after falling into collection assembly 12, and class pest of creeping
It can not climb out of, realize that killing for class pest of creeping is removed, above-mentioned delousing plant can simultaneously kill flying insect and the pest that creeps
It removes.In addition, top deinsectization component and lower part deinsectization component can be split, desinsection is placed according to different planting environment selection combinations
Or individually placed desinsection, and agrochemical need not be used, the quality of sealwort will not be impacted；And only need into
Row fixed point place, you can the pest in environs is attracted to delousing plant and kill removing, it is simple and convenient, save cost and
A large amount of manpower and materials.
In the present invention, guiding net 4 is in a circular table shape, from bottom to top reduced diameter, and multiple connecting poles 9 are along pedestal 1
Foreign aid is uniformly distributed in week.In general, the upper and lower ends of connecting pole 9 are welded with cover net 3 and pedestal 1 respectively.The setting of connecting pole 9 can be with
The entrance of flight class pest is not interfered, ensures insecticidal effect.
In the present invention, lampshade 2 is provided with outside insect attractant light 5, cover plate 2 is in reverse V-shaped, and the top of cover plate 2 is provided with hanging ring.
It is that insect attractant light 5 is destroyed in order to prevent that lampshade 2, which is arranged, by the setting of hanging ring, can be removed in top deinsectization component and lower part
After worm component is split, deinsectization component in top is hung over by hanging ring individually and carries out desinsection suitable for placing the position of desinsection.
In the present invention, collection assembly 12 includes anti-ease bucket and collection box；The setting of anti-ease bucket collection box and collection port it
Between, and anti-ease bucket is funnel-form.
In order to improve the insecticide efficiency in cover net 3, prevent the pest of flight class from surprisingly flying out again by worm inlet hole, usually
Power grid is equipped between cover net 3 and guiding net 4, power grid and direct-flow storage battery 8 are electrically connected.After flight class pest flies into cover net 3
It is directly killed by power grid electricity, improves insecticide efficiency.
In step C1, various ways may be used to the processing mode of sealwort wash seeds sterilizing, in order to make cleaning imitate
Fruit, bactericidal effect reach best, further, in step C1, are used such as to the processing mode of sealwort wash seeds sterilizing
The lower mode：First, sealwort seed is put into bottle, and addition soaked in absolute ethyl alcohol 1 minute in bottle, then sealwort kind
Son taking-up is clean wash with distilled water, and distilled water is then added in bottle and the sealwort seed cleaned up is put into bottle, will
Bottle be placed in 40 DEG C of refrigerators carries out bubble kind processing, it is described bubble kind time be 24-30h, bubble kind after, sealwort seed is taken
Go out to be put into the salicylic acid solution of a concentration of 13.8mg/Ld and impregnate 30min, after will orchardgrass mature seed take out after use distilled water
Cleaning 3-5 times, and sterilizing filter paper is placed it in, dry excessive moisture.Sealwort can also be tentatively abolished by salicylic immersion
The hibernation feature of seed, to lay a good foundation for subsequent culture.
Further, in step C2, the Vb stock that contain in the Fiber differentiation by 20mg/L Vb1,
The niacin of Vb6,9mg/L of 19.5mg/L form.
In order to keep Fiber differentiation effect more preferable, in step C2, the environment temperature of the Fiber differentiation is 25 DEG C, and is cultivated
Mode is dark training.
Further, the N6 culture mediums contained in the Fiber differentiation are by KNO3、NH4SO4、CaCl2·2H2O、
MgSO4·7H2O、KH2PO4、FeSO4·7H2O ZnSO4·4H2O、ZnSO4·7H2O、H2BO3, KI, glycine, niacin, hydrochloric acid
Thiamine, pyridoxine hydrochloride composition, the content of each component are as described below：KNO3For 2830mg/L, NH4SO4 is 463mg/L,
CaCl2·2H2O is 166mg/L, MgSO47H2O is 185mg/L, KH2PO4For 400mg/L, MnSO4·4H2O is 4.4mg/
L, ZnSO4·7H2O is 1.6mg/L, H3BO3For 0.8mg/L, KI 1.6mg/L, glycine 2.0mg/L, niacin 0.5mg/
L, thiamine hydrochloride 1.0mg/L, puridoxine hydrochloride 0.5mg/L.The Vb stock contained in the Fiber differentiation by
The niacin of Vb6,4.5mg/L of Vb1,9.5mg/L of 9.9mg/L form.Contain in the subculture medium, differential medium
MS culture mediums are by KNO3、NH4NO3、MgSO4·7H2O、KH2PO4、CaCl2.2H2O、MnSO4·4H2O、ZnSO4·7H2O、H3BO3、
KI、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·6H2O、Na2-EDTA、FeSO4·4H2O, glycine, thiamine hydrochloride,
Puridoxine hydrochloride, niacin, inositol composition, the content of each component are as described below：KNO3For 1900mg/L, NH4NO3For 1650mg/L,
MgSO4·7H2O is 370mg/L, KH2PO4For 170mg/L, CaCl2·2H2O is 440mg/L, MnSO4·4H2O is 22.3mg/
L, ZnSO4·7H2O is 8.6mg/L, H3BO3For 6.2mg/L, KI 0.83mg/L, NaMoO4·2H2O is 0.25mg/L,
CuSO4·5H2O is 0.025mg/L, CoCl2·6H2O is 0.025mg/L, Na2- EDTA is 37.3mg/L, FeSO4·4H2O is
27.8mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/
L, inositol 100mg/L.
Further, the MS culture mediums include KNO3、NH4NO3、MgSO4·7H2O、KH2PO4、CaCl2·2H2O、
MnSO4·4H2O、ZnSO4·7H2O、H3BO3、KI、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·6H2O、Na2-EDTA、
FeSO4·4H2O, the content of glycine, thiamine hydrochloride, puridoxine hydrochloride, niacin, inositol, sucrose, each component is as described below：
KNO3For 1900mg/L, NH4NO3For 1650mg/L, MgSO47H2O is 370mg/L, KH2PO4For 170mg/L, CaCl2·2H2O
For 440mg/L, MnSO4·4H2O is 22.3mg/L, ZnSO4·7H2O is 8.6mg/L, H3BO3For 6.2mg/L, KI 0.83mg/
L, NaMoO4·2H2O is 0.25mg/L, CuSO4·5H2O is 0.025mg/L, CoCl2·6H2O is 0.025mg/L, Na2-EDTA
For 37.3mg/L, FeSO4·4H2O is 27.8mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.1mg/L, and hydrochloric acid pyrrole is trembled
Alcohol is 0.5mg/L, niacin 0.5mg/L, inositol 100mg/L, sucrose 30g/L.
Claims (10)
1. a kind of sealwort cultivating and growing method, it is characterised in that include the following steps：
A, it reserves seed for planting；Sealwort seed carries out Sand culture immediately in annual August maturation, the seed for choosing mature and plump：1 part of seed,
3 parts of sand is uniformly mixed, and is stored in the hole of 30 centimetres of depths of shady spot, and moistening is kept；
B, selection of land, site preparation；Selection moistens and has the plot fully covered, and soil is loose with quality, moisture holding capacity good loam or sand
Loam is advisable, and is first ploughed deeply 1 time before sowing, applies 2000 kilograms of farm manure per acre in conjunction with site preparation, plough under and make base manure in soil, and then rake is thin
Leveling, bedding, furrow are 1.2 meters wide.
C, sealwort seed seedling-raising：
C1, seed selection：Sealwort seed is chosen, and is carried out disinfection processing to sealwort seed；
C2, the sealwort seed after washing and sterilizing is inoculated into inducing culture and cultivates 26~30d in an aseptic environment and is cured
Injured tissue, the inducing culture is by N6 culture mediums, IAA, 300mg/LETH, 500mg/L of GA3,100mg/L of 150mg/L
SNP, Vb stock, the sucrose of 30g/L, 5.0mg/L 2, the hydrogen peroxide of 4-D, 100mg/L, 4.5g/L agar composition, institute
State the PH=5.8 of inducing culture；
C3, callus is transferred in subculture medium and cultivates 5~9d in an aseptic environment, the subculture medium is cultivated by MS
The 2 of base, the sucrose of 30g/L, 5.0mg/L, the potassium permanganate of 6-BA, 100mg/L of 4-D, 100mg/L, the activated carbon of 1.0g/L,
The agar of 4.5g/L forms, the PH=5.8 of the subculture medium；
C4, it will be transferred in differential medium by the callus of step C3 processing and cultivate 18~22d in an aseptic environment, institute
State differential medium by MS culture mediums, the sucrose of 30g/L, 0.2mg/L NAA, 50mg/L GA3,1g/L copper sulphate,
The agar of 6-BA, 4.5g/L of 100mg/L form, the PH=5.8 of the differential medium；
C5, it will be transferred in tissue culture bottle by the callus of step C4 processing, and tissue culture bottle be placed on intensity of illumination and is
1500~3000lx, for temperature to cultivate 5~8d in 25~30 DEG C of gnotobasis, light application time is 38~42h/d；
C6, tissue culture bottle is placed on to temperature it is 20~30 DEG C and have 4~7d of hardening culture in collarium border, in hardening incubation
In, apply outer source additive in seedling root, the outer source additive includes solvent and solute, and the solvent is Huo Gelanshiying
Nutrient solution, the solute include chitosan (CTS) and spermine (Spm), a concentration of 0.5g/L-1.5g/L of the CTS, the Spm
A concentration of 50.58mg/L-151.74mg/L；
C7, when seedling grows to 2~3cm, be transplanted in whole good field；
D, field management：Early period is wanted frequent tillage and weeding in sealwort growth, is respectively carried out 1 time 4,6,9, November every year, and preferably shallow hoe is simultaneously
Appropriate ridging, later stage uprooting weed；Top dressing is carried out during tillage and weeding, applies farmyard manure 1500 after 3 times intertilling per acre
Kilogram, and 50 kilograms of calcium superphosphate, 50 kilograms of cake fertilizer, mixing mix thoroughly after after ditching-fertilizing in the ranks, fertilising earthing lid fertilizer；Sealwort
It needs to irrigate in time in growth course；Keep ground moistening；
E, pest control；Deinsectization is carried out using physical deinsectization method.
2. sealwort cultivating and growing method as described in claim 1, it is characterised in that：In step D, sealwort is irrigated using such as
Lower irrigation system：The irrigation system include multiple plantation modules (100), water tank (200) and with the water tank (200)
The irrigation conduit of connection；The irrigation conduit includes spray header (300) and sprinkling irrigation water pipe (400)；The spray header (300)
On be communicated with multiple spray heads (500), the spray head (500) is located at the tops of multiple plantation modules (100)；The spray
Irrigation pipe (400) is communicated with multiple rainers (600) in earth's surface；The rainer (600) includes accommodating to manage
(601), multiple soakaway trench (602), Multi-direction sprinkling irrigation head (603) and jacking cylinder (604)；The Multi-direction sprinkling irrigation head (603) and institute
Sprinkling irrigation water pipe (400) is stated to be connected；The receiving pipe (601) and multiple soakaway trench (602) are embedded under earth's surface；It is described
Multi-direction sprinkling irrigation head (603) setting receivings pipe (601) in, and can be stretched out under the driving of the jacking cylinder (604) or
Take in the receiving pipe (601)；The soakaway trench (602) manages (601) with the receiving and is connected, and is managed along the receiving
(601) extend radially outwardly；Multiple water seepage holes (605) are offered on the soakaway trench (602)；Described accommodate is managed (601)
Bottom offers through-hole, and the lifting pin of the jacking cylinder (604) is connected to the Multi-direction sprinkling irrigation head (603) across the through-hole
Bottom, further include sun-shading mechanism (700)；The sun-shading mechanism (700) includes puggaree (701), frame (702) and driving electricity
Machine (703)；The puggaree (701) is fixed in the frame (702), is arranged in the drive shaft of the driving motor (703)
There is driving gear, driven tooth corresponding with the driving gear, the driven tooth and the drive are offered on the frame (702)
Moving gear is meshed, and multiple loopholes identical with spray head (500) size are offered on the puggaree (701), described
The position of unthreaded hole is corresponding with the position of the spray head (500).
3. sealwort cultivating and growing method as claimed in claim 2, it is characterised in that：The planting rhizoma polygonati irrigation system further includes
Multiple control valves, controller, illuminance sensor and the humidity sensor for detecting surface humidity；Multiple control valves point
It is not correspondingly arranged on the spray header (300) and the sprinkling irrigation water pipe (400)；The controller respectively with the control
Valve, the intensity of illumination sensor and humidity sensor electrical connection；The controller is according to the intensity of illumination sensor
Induction adjust the position of the puggaree (701)；The controller controls the control according to the induction of the humidity sensor
The sprinkling irrigation mode of the open and close of valve processed and the rainer (600).
4. sealwort cultivating and growing method as claimed in claim 2, it is characterised in that：The soakaway trench (602) and the jacking
For bar directly by seal with elastometic washer, the length of the soakaway trench (602) is 400-800mm；The water seepage hole (605) it is a diameter of
3-5mm, and the spacing between the adjacent water seepage hole (605) is 10-20mm, the receiving pipe (601) and the soakaway trench
(602) connectivity part is equipped with filter screen.
5. sealwort cultivating and growing method as described in claim 1, it is characterised in that：In step E, the physical deinsectization method
It is realized using following delousing plant：The delousing plant includes top deinsectization component and lower part deinsectization component；The top deinsectization
Component includes pedestal (1), cover plate (2), cover net (3), guiding net (4), insect attractant light (5) and heat conductive rod (6)；The insect attractant light (5)
It is fixed at the center of the pedestal (1), the insect attractant light (5) is internally provided with heat conductive rod (6)；The cover plate (2) is located at
Above the cover net (3), cover plate (2) upper end is equipped with solar panel (7)；Direct current electric power storage is equipped in the pedestal (1)
Pond；The solar panel (7), the insect attractant light (5) and the heat conductive rod (6) pass through conducting wire and the direct current electric power storage respectively
Pond is connected；The bottom of the cover net (3) is connected with the bottom of the guiding net (4), offered at the top of the guiding net (4) into
Worm channel；The cover net (3) is connected with the pedestal (1) by multiple connecting poles (9)；The lower part deinsectization component includes connected unit
(10), slope (11), collection assembly (12) and volatilization room (13) are guided；The connected unit (10) and the pedestal (1) detachably connect
It connects；Guiding slope (11) is integrally formed around the periphery for being located at the connected unit (10) and with the connected unit (10)；It is described to wave
It is internal in the connected unit (10) to send out room (13) setting, attractant (14) is placed in the volatilization room (13)；The connected unit
(10) multiple evaporation holes are offered on, multiple evaporation holes are connected with the volatilization room (13)；The collection assembly (12)
Be arranged it is described guiding slope (11) and the connected unit (10) inside, and offered on the guiding slope (11) it is multiple with it is described
The collection port of collection assembly (12) connection.
6. sealwort cultivating and growing method as claimed in claim 5, it is characterised in that：The guiding net (4) is in a circular table shape, under
Upward reduced diameter, multiple connecting poles (9) are uniformly distributed in week along the foreign aid of the pedestal (1).
7. sealwort cultivating and growing method as claimed in claim 5, it is characterised in that：It is provided with lampshade outside the insect attractant light (5),
The cover plate (2) is in reverse V-shaped, and is provided with hanging ring at the top of the cover plate (2).
8. sealwort cultivating and growing method as claimed in claim 5, it is characterised in that：The collection assembly (12) includes anti-ease bucket
And collection box；The anti-ease bucket is arranged between the collection box and the collection port, and the anti-ease bucket is funnel-form.
9. sealwort cultivating and growing method as described in claim 1, it is characterised in that：In step C1, to sealwort wash seeds
The processing mode of sterilizing is by the way of as described below：First, sealwort seed is put into bottle, and adds absolute ethyl alcohol in bottle
Impregnate 1 minute, then sealwort seed takes out clean wash with distilled water, and distilled water is then added in bottle and will clean up
Sealwort seed is put into bottle, and bottle is placed in 40 DEG C of refrigerators and carries out bubble kind processing, and the time of the bubble kind is 24-30h, bubble kind
After, sealwort seed is taken out to be put into the salicylic acid solution of a concentration of 13.8mg/Ld and impregnates 30min, after by orchardgrass maturation
Seed is cleaned 3-5 times after taking out with distilled water, and places it in sterilizing filter paper, dries excessive moisture.
10. sealwort cultivating and growing method as described in claim 1, it is characterised in that：In step C2, in the Fiber differentiation
The Vb stock contained are made of the niacin of Vb6,9mg/L of Vb1,19.5mg/L of 20mg/L.
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| CN201810554205.2A CN108782045A (en) | 2018-06-01 | 2018-06-01 | A kind of sealwort cultivating and growing method |
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