CN108742528A - A kind of the fast linear cofocus scanning optoacoustic probe and its imaging method of no water coincidence - Google Patents
A kind of the fast linear cofocus scanning optoacoustic probe and its imaging method of no water coincidence Download PDFInfo
- Publication number
- CN108742528A CN108742528A CN201810527842.0A CN201810527842A CN108742528A CN 108742528 A CN108742528 A CN 108742528A CN 201810527842 A CN201810527842 A CN 201810527842A CN 108742528 A CN108742528 A CN 108742528A
- Authority
- CN
- China
- Prior art keywords
- ultrasonic
- coupler
- light
- ultrasonic coupler
- shell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0093—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy
- A61B5/0095—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy by applying light and detecting acoustic waves, i.e. photoacoustic measurements
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
Abstract
The invention discloses a kind of fast linear cofocus scanning optoacoustic of no water coincidence probe and imaging method, optoacoustic probe includes casing, optical fiber collimator, galvanometer, flat field scanning lens, hollow two-dimension translational adjustment frame, ultrasonic coupler, ultrasonic transducer；From the laser of fiber exit after optical fiber collimator collimates, by vibration mirror reflected, then flat field scanning lens, hollow two-dimension translational adjustment frame, ultrasonic coupler are injected successively and reaches sample.After sample absorbs light, acoustical signal is generated, electric signal is converted to by ultrasonic transducer, be passed to computer processing system, fast image reconstruction is then carried out by GPU, realizes real time imagery.The invention has the advantages that while ensuring high-resolution using an excitation, quickly linear cofocus scanning detection is realized, and the device without water coincidence can reach more preferably effect in the application aspect of matching optoacoustic microsurgical instrument.
Description
Technical field
The present invention relates to photoacoustic imaging technology and device fields, and in particular to a kind of fast linear of no water coincidence is confocal to be swept
Retouch optoacoustic probe and its imaging method.
Background technology
Photoacoustic imaging is the new bio medical imaging side of a kind of non-invasive and unionized formula developed in recent years
Method.When in pulsed laser irradiation to (thermal acoustic imaging then refers in particular to be irradiated with the pulse laser of radio frequency) biological tissue
When, the light absorption domain of tissue will generate ultrasonic signal, our this ultrasonic signals generated by light excitation are referred to as photoacoustic signal.It is raw
The photoacoustic signal that object tissue generates carries the light absorption characteristics information of tissue, can be reconstructed in tissue by detecting photoacoustic signal
Light absorption distributed image.It is deep in being imaged that photoacoustic imaging combines high selection characteristic and pure ultrasonic tissue in pure optical texture imaging
The advantages of through characteristic, the organization chart picture of high-resolution and high contrast can be obtained, the influence of light scattering is avoided from principle,
High resolution optical imaging depth " soft limiting " (~1mm) is breached, it can be achieved that the deep layer in vivo tissue of 50mm is imaged.
Since photoacoustic imaging is to receive photoacoustic signal using ultrasonic transducer, photoacoustic signal travels to ultrasound from biological tissue
Energy converter is needed by coupling substance.The optoacoustic probe of the overwhelming majority is to do coupling substance with water, but make coupling with water at present
Close substance slightly has some bubbles there are many disadvantage first, cannot have bubble in water, may result in the distortion of photoacoustic signal, this
Just there is high requirement to the leakproofness of imaging device, is difficult to reach in engineering；Second is that allow to reach sealing requirements,
Water after a certain time can be dirty, goes bad, this is unavoidable, this results in carrying out all needing before photoacoustic imaging detection every time
Change water.This considerably increases the triviality of photoacoustic imaging detection operationally.
In addition, traditional photoacoustic imaging system is difficult to realize fast imaging and high-resolution imaging simultaneously.It is quick to reach
Imaging, traditional photoacoustic imaging system use large spot irradiating sample, signal are acquired with ultrasound transducer array, although energy in this way
Reach real time imagery, but resolution ratio is limited by array elements size, cannot achieve high-resolution imaging；To reach high-resolution
Rate needs to focus the light into, and resolution ratio depends on the size of focal beam spot, but after light focuses, light only irradiates one piece of minimum area
Domain then just needs to carry out optical scanning to be imaged to entire target area, and traditional photoacoustic imaging system is driven using motor and visited
Head or sample movement carry out optical scanning, and motor quality is big, inertia is big, can not be achieved quick scanning substantially, even if realizing
The quick scanning of light, traditional acousto-optic imaging method handle data due to the use of slow CPU, it is also difficult to real time imagery.
Invention content
The shortcomings that it is a primary object of the present invention to overcome the prior art and deficiency, provide a kind of Express Order Wire of no water coincidence
Property cofocus scanning optoacoustic probe, the optoacoustic probe use solid ultrasonic coupling substance as couple substance, not only substantially reduce
The triviality of photoacoustic imaging detection operationally, does not interfere with imaging effect also.
Another object of the present invention is to provide a kind of imagings of the fast linear cofocus scanning optoacoustic of no water coincidence probe
Method realizes high-resolution real time imagery.
In order to reach above-mentioned first purpose, the present invention uses following technical scheme：
A kind of fast linear cofocus scanning optoacoustic of no water coincidence proposed by the present invention is popped one's head in, including casing, fiber optic collimator
Device, galvanometer, flat field scanning lens, hollow two-dimension translational adjustment frame, ultrasonic coupler and ultrasonic transducer；The casing packet
One piece of tabletting and chassis body are included, the chassis body is equipped with mounting hole, is respectively used to installation galvanometer, optical fiber collimator, flat field
Scanning lens and hollow two-dimension translational adjustment frame；The ultrasonic coupler and ultrasonic transducer setting are adjusted in hollow two-dimension translational
On frame；
The ultrasonic coupler includes the top of ultrasonic coupler, the lower part of ultrasonic coupler and the anti-acoustic mirror of light transmission, described
The light incident surface and light-emitting face of ultrasonic coupler are horizontally disposed, ensure vertical incidence, the outgoing of light, avoid the inclined of light
Folding, the anti-acoustic mirror of light transmission are obliquely installed from the horizontal by 45 degree, and positioned at the top of ultrasonic coupler, the lower part of ultrasonic coupler
Centre, the top of the ultrasonic coupler, the anti-acoustic mirror of light transmission, ultrasonic coupler lower part closely connect successively；
The ultrasonic transducer is arranged on the lower part of ultrasonic coupler, after connection, the flat field scanning lens,
Hollow two-dimension translational adjustment frame, ultrasonic coupler are sequentially coaxially arranged.
The mounting hole is configured as threaded hole as a preferred technical solution,；The optical fiber collimator peace field scan
Lens are threaded itself, are directly connected by the threaded hole to match on chassis body；The galvanometer passes through screw holddown belt
Porose tabletting is fixed on chassis body；The chassis body is connected with hollow two-dimension translational adjustment frame by the screw of four corners
It connects.
The optical fiber collimator is cylindric as a preferred technical solution, and one end carries external screw thread, to casing
On threaded hole cooperation fix, the other end is equipped with the helicitic texture that is of coupled connections with optical fiber head.
The galvanometer includes smooth coated reflective eyeglass, drives the circle of eyeglass deflection as a preferred technical solution,
Column motor and signal wire, after the power is turned on, coated reflective eyeglass are locked in initial position, by coated reflective eyeglass with level at 45
Angle setting is spent, makes the collimated light being emitted from optical fiber collimator through the vertical incident flat field scanning lens of its reflection, after galvanometer is set,
Using tabletting, the galvanometer regulated is compressed by screw, galvanometer control module drives eyeglass to deflect by signal line traffic control motor,
The deflection angle of eyeglass is directly proportional to the control amplitude of voltage.
The flat field scanning lens possesses the function of focus on light beam as a preferred technical solution, and laser is made to scan
Region inner focusing is minimum luminous point, realizes high-resolution；Flat field scanning lens can also solve to lead when galvanometer wide-angle fan sweeping
The optical focus of cause is not the same level the problem of, and when this to scan sample surfaces, the focal beam spot of scanning area periphery is big
It is small almost the same with the focal beam spot at center, to ensure that the lateral resolution in entire scanning area is optimum resolution；
Since flat field scanning lens is threadedly secured on casing, it is axially adjustable that rotary flat field scan lens may be implemented optical focus,
To realize the confocal excitation of light, sound and detection mode.
The hollow two-dimension translational adjustment frame for adjusting in sound field in the horizontal plane as a preferred technical solution,
The relative position of the heart and light realizes that optoacoustic is coaxial；The hollow two-dimension translational adjustment includes outside hollow two-dimension translational adjustment frame
Shell, adjusting screw rod and the translatable part with hollow round hole carry out the two-dimensional directional on horizontal plane by two adjusting screw rods
It adjusts, is threaded on the hollow round hole of translatable part, to connect ultrasonic coupler and ultrasonic transducer.
The top of the ultrasonic coupler includes the shell of ultrasonic coupler upper section as a preferred technical solution,
With the solid ultrasonic coupling substance inside first；The top half of the shell of the ultrasonic coupler upper section is cylinder,
With external thread structure, to be connect with the translatable part of hollow two-dimension translational adjustment frame, ultrasonic coupler upper section
The upper surface of shell is horizontal plane, and light is from upper surface vertical incidence；The lower half of the shell of the ultrasonic coupler upper section
Be divided into prism, lower surface be one from the horizontal by 45 degree of angles inclined-plane；The axle center part of the shell of ultrasonic coupler upper section
The square three-way groove being divided into axial direction, the first solid ultrasonic coupling substance to match with straight slot shape and ultrasonic coupler upper section
Shell closely connect；
The lower part of the ultrasonic coupler includes that the solid inside the shell and second of ultrasonic coupler section below is super
Acoustical coupling substance, the shell of the ultrasonic coupler section below are in integrally prism-shaped, the left side of shell be from the horizontal by
The inclined-plane at 45 degree of angles matches with the lower surface on the top of ultrasonic coupler；There are two the shells of ultrasonic coupler section below
Straight slot, one is located on the left of the shell of ultrasonic coupler section below, is axial square three-way groove, with the top of ultrasonic coupler
Straight slot matches, and the second solid ultrasonic coupling substance and the shell of ultrasonic coupler section below to match with straight slot shape is close
Connection；Another runs through straight slot for the rectangular of horizontal direction, for matching connection with ultrasonic transducer.
The anti-acoustic mirror thickness of light transmission is less than 0.1mm, light transmittance 92% or more and table as a preferred technical solution,
Face is smooth；
The first solid ultrasonic coupling substance and the second solid ultrasonic coupling substance clear, colorless, light transmittance is 88%
More than, the light plane of incidence of the first, second solid ultrasonic coupling substance, the face for contacting sample, the face being connect with ultrasonic transducer
And the face contacted with the anti-acoustic mirror of light transmission is processed by shot blasting, reduces the decaying of light, sound in communication process, other faces are done
At frosting, reduce external unrelated light, influence of the sound to experiment.
The ultrasonic transducer includes ultrasonic transducer shell, matching layer, piezoresistive material as a preferred technical solution,
Material, backing block and signal wire, the ultrasonic transducer shell are prism, and size matches the logical of ultrasonic coupler lower horizontal direction
One end of ultrasonic transducer reception/transmitting ultrasound is directed at the anti-acoustic mirror of light transmission by slot, after so that sound field is passed through the anti-acoustic mirror reflection of light transmission with
The top of ultrasonic coupler is coaxial, will be in one end of energy converter reception/transmitting ultrasound and ultrasonic coupler lower part axial pass trough
Solid ultrasonic coupling substance closely connects, and the focal length parameter of the ultrasonic transducer is set as focal plane and optoacoustic probe bottom
Solid ultrasonic coupling material surface overlap, the electric signal that ultrasonic transducer obtains passes to computer processing system through signal wire.
In order to reach above-mentioned second purpose, the present invention uses following technical scheme：
A kind of imaging method of the fast linear cofocus scanning optoacoustic probe of no water coincidence proposed by the present invention, including it is following
Step：
(1) sample surface to be measured applies a little ultrasonic coupling liquid, is affixed on the solid ultrasonic coupling object of optoacoustic probe bottom
Matter surface；
(2) galvanometer powers on, laser emitting module shoot laser；
(3) size of signal, rotary flat field scan lens, ultrasonic coupler and the adjustment of hollow two-dimension translational are swept according to A
The adjusting screw rod of frame, to adjust the relative position of optical focus and ultrasonic transducer focal plane, realize light, sound it is confocal with high-resolution at
Picture, and optical focus is made to be located just at sample surfaces；
(4) computer processing system gives galvanometer control module and signal acquisition module synchronous triggering signal simultaneously, to carry out
The line scanning of light and parallel acquisition, the storage of photoacoustic signal；
(5) collected photoacoustic signal is quickly reconstructed reflection sample different structure using GPU by computer processing system
To the photoacoustic image of laser difference absorbing state, real time imagery.
Compared with prior art, the present invention having the following advantages that and advantageous effect：
1, the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence of the invention, uses solid ultrasonic coupling substance
As coupling substance, the triviality of photoacoustic imaging detection operationally is not only substantially reduced, does not interfere with imaging effect also；It should
Optoacoustic probe using galvanometer realize 10mm or more a wide range of optical scanning, and the sweep speed of galvanometer can meet completely in real time at
The demand of picture；Caused optical focus is not in same level when can solve galvanometer wide-angle fan sweeping using flat field scanning lens
Problem, when this to scan sample, the focal beam spot size of scanning area periphery and the focal beam spot at center are almost the same, to
Ensure the high-resolution in entire scanning area.
2, computer processing system of the invention carries out rapid image weight by GPU to the photoacoustic signal of optoacoustic probe outflow
It builds, realizes real time imagery.
3, ultrasonic transducer of the present invention uses reflective reception mode, the light for generating biological tissue using the anti-acoustic mirror of light transmission
Acoustic signal back is changed with hollow ultrasonic transducer, light is used from hollow ultrasound to the ultrasonic transducer for being located at optoacoustic probe side
It passes through and is irradiated on sample among energy device, the mode that hollow ultrasonic transducer directly receives photoacoustic signal is compared, and use is reflective
The benefit of reception, which is scanning range, to be limited by the hollow aperture of hollow ultrasonic transducer.
4, the present invention replaces motor to carry out optical scanning using galvanometer, reduces volume, weight, cost and the work of optoacoustic probe
Make noise.
Description of the drawings
Fig. 1 is a kind of fast linear cofocus scanning optoacoustic probe face structural schematic diagram figure of no water coincidence of the present invention.
Fig. 2 (a), Fig. 2 (b) be respectively a kind of no water coincidence of the present invention fast linear cofocus scanning optoacoustic probe before,
Left sectional view.
Fig. 3 is a kind of solidworks design drawings of the fast linear cofocus scanning optoacoustic probe of no water coincidence of the present invention.
Fig. 4 is to utilize the photoacoustic imaging result that optoacoustic probe and imaging method obtain described in embodiment.
Wherein, 1 is casing, and 1-1 is chassis body, and 1-2 is tabletting, and 2 be galvanometer, and 2-1 is galvanometer eyeglass, and 2-2 is galvanometer
Motor, 2-3 are galvanometer signal wire, and 3 be optical fiber collimator, and 4 be flat field scanning lens, and 5 be hollow two-dimension translational adjustment frame, 5-1
Rack enclosure is adjusted for hollow two-dimension translational, 5-2 is adjusting screw rod, and 5-3 is translatable part, and 6 ultrasonic couplers, 6-1 is ultrasound
The top of coupler, 6-2 are the lower part of ultrasonic coupler, and 6-1-1 is the shell of ultrasonic coupler upper section, and 6-2-1 is super
The shell of acoustic coupler section below, 6-1-2, the first solid ultrasonic coupling substance, 6-2-2 are the second solid ultrasonic coupling object
Matter, 6-3 are the anti-acoustic mirror of light transmission, and 7 be ultrasonic transducer, and 7-1 is ultrasonic transducer shell, and 7-2 is ultrasonic transducer matching layer, 7-
3 be ultrasonic transducer piezoelectric material, and 7-4 is ultrasonic transducer backing block, and 7-5 is ultrasonic transducer signal wire.
Specific implementation mode
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
Embodiment
As shown in Figure 1, Figure 2 shown in (a) Fig. 2 (b), Fig. 3, the present invention discloses a kind of fast linear cofocus scanning light of no water coincidence
Sonic probe, including：Including casing 1, galvanometer 2, optical fiber collimator 3, flat field scanning lens 4, hollow two-dimension translational adjustment frame 5, surpass
Acoustic coupler 6 and ultrasonic transducer 7；The casing includes one piece of tabletting and chassis body, and the chassis body is equipped with peace
Hole is filled, installation galvanometer, optical fiber collimator, flat field scanning lens and hollow two-dimension translational adjustment frame are respectively used to；The ultrasound coupling
Clutch and ultrasonic transducer are arranged on hollow two-dimension translational adjustment frame.
The effect of the casing 1 is to connect other various pieces；The effect of the galvanometer 2 is to realize laser in ultrasonic transduction
Fast linear scanning is carried out in the sound field focusing region of device；The effect of the optical fiber collimator 3 is will be from the diverging of fiber exit
Light is collimated；The effect of the flat field scanning lens 4 is the excitation and detection mode for realizing that light, sound are confocal；Described hollow two
The effect of dimension translation adjustment frame 5 is to adjust the position at sound field center；The sound that the effect of the ultrasonic coupler 6 generates sample is believed
Number pass to ultrasonic transducer；The effect of the ultrasonic transducer 7 is that acoustical signal is converted to electric signal.
In conjunction with Fig. 2 (a) Fig. 2 (b) and Fig. 3, more specifically, optoacoustic of the invention probe includes chassis body 1-1, tabletting
1-2, galvanometer eyeglass 2-1, galvanometer motor 2-2, galvanometer signal wire 2-3, optical fiber collimator 3, flat field scanning lens 4, hollow two dimension
The shell 6-1-1, super of translation adjustment rack enclosure 5-1, adjusting screw rod 5-2, translatable part 5-3, ultrasonic coupler upper section
The shell 6-2-1 of acoustic coupler section below, the first solid ultrasonic coupling substance 6-1-2, the second solid ultrasonic coupling substance 6-
2-2, the anti-acoustic mirror 6-3 of light transmission, ultrasonic transducer shell 7-1, ultrasonic transducer matching layer 7-2, ultrasonic transducer piezoelectric material 7-
3, ultrasonic transducer backing block 7-4 and ultrasonic transducer signal wire 7-5.
To the present invention, the structure of the fast linear cofocus scanning optoacoustic probe without water coincidence is further elaborated below：
The casing 1 includes chassis body 1-1 and one piece of tabletting 1-2, and chassis body 1-1 has is threaded hole everywhere,
It is respectively used to connection galvanometer 2, optical fiber collimator 3, flat field scanning lens 4 and hollow two-dimension translational adjustment frame 5；Optical fiber collimator 3
Peaceful field scan lens 4 are threaded itself, are directly connected by the threaded hole to match on chassis body 1-1；Galvanometer 2 is logical
The porose tabletting 1-2 of screw holddown belt is crossed to be fixed on chassis body 1-1；Chassis body 1-1 and hollow two-dimension translational adjustment frame 5
Pass through the screw connection of four corners；After connection, flat field scanning lens 4 and hollow two-dimension translational adjustment frame 5 are coaxial.
The galvanometer 2 is by smooth coated reflective eyeglass 2-1, the cylindric motor 2-2 and signal that drive eyeglass to deflect
Line 2-3 compositions, after the power is turned on, eyeglass 2-1 are locked in initial position, and eyeglass is arranged with horizontal at 45 degree of angles, is made from optical fiber standard
The collimated light that straight device 3 is emitted after galvanometer 2 is set, using tabletting 1-2, passes through through its reflection vertical incident flat field scanning lens 4
Screw compresses the galvanometer 2 regulated, and galvanometer control module controls motor 2-2 by signal wire 2-3 and drives galvanometer eyeglass 2-1 inclined
Turn, the deflection angle of galvanometer eyeglass 2-1 is directly proportional to the control amplitude of voltage, and range of deflection is positive and negative 10 degree or more, working frequency
Up to 1KHz or more, it can be achieved that the Express Order Wire of the 10mm range above of light is swept.
The optical fiber collimator 3 is cylindric, and one end carries external screw thread, to solid with the threaded hole cooperation on casing 1
Fixed, the other end is equipped with the helicitic texture being of coupled connections with optical fiber head.
The flat field scanning lens 4 possesses the function of focus on light beam, make laser scanning area inner focusing be it is minimum
Luminous point, therefore realize high-resolution；Flat field scanning lens 4 can also solve when 2 wide-angle fan sweeping of galvanometer caused optical focus not
The same level the problem of, when this to scan sample surfaces, the focal beam spot size of scanning area periphery and gathering for center
Burnt hot spot is almost the same, to ensure that the lateral resolution in entire scanning area is optimum resolution；Since flat field scans
Lens 4 are threadedly secured on casing 1, and it is axially adjustable that rotary flat field scan lens 4 may be implemented optical focus, to realize
The confocal excitation of light, sound and detection mode.
The hollow two-dimension translational adjustment frame 5 by its hollow two-dimension translational adjustment rack enclosure 5-1, adjusting screw rod 5-2 and
Translatable part 5-3 compositions with hollow round hole, the two-dimensional directional tune on horizontal plane is carried out by two adjusting screw rod 5-2
It saves, is threaded on the hollow round hole of translatable part 5-3, to connect ultrasonic coupler 6 and ultrasonic transducer 7.Described
The effect of hollow two-dimension translational adjustment frame 5 is the relative position at the center and light that adjust sound field in the horizontal plane, realizes that optoacoustic is same
Axis.
The ultrasonic coupler 6 is by the top 6-1 of ultrasonic coupler, two sections of the lower part 6-2 of ultrasonic coupler and light transmission
Anti- acoustic mirror 6-3 compositions, the light incident surface and light-emitting face of ultrasonic coupler 6 are horizontally disposed, ensure the vertical incidence of light, go out
It penetrates, avoids the deviation of light, the anti-acoustic mirror 6-3 of light transmission is obliquely installed from the horizontal by 45 degree, and positioned at the super of ultrasonic coupler
The centre of the top 6-1 of acoustic coupler, two sections of the lower part 6-2 of ultrasonic coupler, three are closely connected by extraordinary glue.
The top 6-1 of the ultrasonic coupler is by the shell 6-1-1 of its ultrasonic coupler upper section and internal the
One solid ultrasonic coupling substance 6-1-2 compositions.The shell 6-1-1 top halfs of ultrasonic coupler upper section are cylinder, are carried
External thread structure, to be connect with the translatable part 5-3 of hollow two-dimension translational adjustment frame, outside ultrasonic coupler upper section
The upper surface of shell 6-1-1 is horizontal plane, and light is from upper surface vertical incidence；The shell 6-1-1 lower half of ultrasonic coupler upper section
Part be prism, lower surface be one from the horizontal by 45 degree of angles inclined-plane；The shell 6-1-1's of ultrasonic coupler upper section
Axial portions are the square three-way groove in axial direction, and the first solid ultrasonic coupling substance 6-1-2 to match with straight slot shape passes through special type
Glue is closely connect with the shell 6-1-1 of ultrasonic coupler upper section.
The lower part 6-2 of the ultrasonic coupler is by the shell 6-2-1 of its ultrasonic coupler section below and internal the
Two solid ultrasonic coupling substance 6-2-2 compositions.The shell 6-2-1 of ultrasonic coupler section below is in integrally prism-shaped, ultrasonic coupling
The left side of the shell 6-2-1 of clutch section below is the inclined-plane from the horizontal by 45 degree of angles, the top 6-1 with ultrasonic coupler
Lower surface match.There are two straight slot, one is located on the left of shell the shell 6-2-1 of ultrasonic coupler section below, is axis
To square three-way groove, match with the straight slot of the top 6-1 of ultrasonic coupler, the second solid ultrasonic coupling to match with straight slot shape
Substance 6-2-2 is closely connect by extraordinary glue with the shell 6-2-1 of ultrasonic coupler section below；Another is horizontal direction
It is rectangular run through straight slot, for ultrasonic transducer 7 matching connection.
The anti-acoustic mirror 6-3 thickness of the light transmission be less than 0.1mm, light transmittance 92% or more and surface it is smooth, the present invention choosing
The anti-acoustic mirror 6-3 of light transmission is but not limited to sheet glass.
The first solid ultrasonic coupling substance 6-1-2, the second solid ultrasonic coupling substance 6-2-2 clear, colorless, thoroughly
Light rate is 88% or more, and physico-chemical property stabilization, and acoustic impedance harmless to biological tissue are between tissue and ultrasonic transducer
With between layer 7-2, the first solid ultrasonic coupling substance 6-1-2, the light incidence of the second solid ultrasonic coupling substance 6-2-2 are flat
Face, the face for contacting sample are polished place with the face of the connection of ultrasonic transducer 7 and with the faces contacted the anti-acoustic mirror 6-3 of light transmission
Reason reduces the decaying in communication process of light, sound, and other faces make frosting, reduces external unrelated light, sound to the shadow of experiment
It rings, the first solid ultrasonic coupling substance 6-1-2, the second solid ultrasonic coupling substance 6-2-2 that the present invention selects are but not limited to
Polystyrene (PS).
The ultrasonic transducer 7 is by its ultrasonic transducer shell 7-1, ultrasonic transducer matching layer 7-2, ultrasonic transducer
Piezoelectric material 7-3, ultrasonic transducer backing block 7-4 and ultrasonic transducer signal wire 7-5 compositions, ultrasonic transducer shell 7-1 are
Prism, size matches the straight slot of ultrasonic coupler lower part 6-2 horizontal directions, by one end of 7 receptions of ultrasonic transducer/transmitting ultrasound
It is directed at the anti-acoustic mirror 6-3 of light transmission, it is coaxial with the top 6-1 of ultrasonic coupler after so that sound field is passed through the anti-acoustic mirror 6-3 reflections of light transmission, it is used in combination
Extraordinary glue consolidates one end of 7 receptions of ultrasonic transducer/transmitting ultrasound with second in the 6-2 axial pass troughs of ultrasonic coupler lower part
Body ultrasonic coupling substance 6-2-2 is closely connected, and the focal length parameter of the ultrasonic transducer 7 is set as focal plane and pops one's head in optoacoustic
Second surfaces solid ultrasonic coupling substance 6-2-2 of bottom overlap, and the electric signal that ultrasonic transducer 7 obtains is believed through ultrasonic transducer
Number line 7-5 passes to computer processing system, and the ultrasonic transducer 7 that the present invention selects is but not limited to line focus ultrasonic transducer.
In the present invention, the flat field scanning lens 4, hollow two-dimension translational adjustment frame 5, ultrasonic coupler 6 are same successively
Axis is arranged.
In the present embodiment, using the imaging method that above-mentioned optoacoustic is popped one's head in, steps are as follows：
(1) sample surface to be measured applies a little ultrasonic coupling liquid, is affixed on the second solid ultrasound coupling of optoacoustic probe bottom
Close the surfaces substance 6-2-2；
(2) galvanometer 2 powers on, laser emitting module shoot laser；
(3) size of signal, rotary flat field scan lens 4, ultrasonic coupler 6 and hollow two-dimension translational tune are swept according to A
The adjusting screw rod of whole frame 5 realizes that light, sound ray are confocal to adjust the relative position of optical focus and 7 focal line of line focus ultrasonic transducer
With high-resolution imaging, and optical focus is made to be located just at sample surfaces；
(4) computer processing system gives galvanometer control module and signal acquisition module synchronous triggering signal simultaneously, to carry out
The parallel acquisition of optical scanning and photoacoustic signal, storage；
(5) collected photoacoustic signal is quickly reconstructed reflection sample different structure using GPU by computer processing system
To the photoacoustic image of laser difference absorbing state, real time imagery.
Fig. 4 is to utilize the photoacoustic imaging result that optoacoustic probe and imaging method obtain described in embodiment.Wherein the left side is sample
Product (faces XY), the right are that corresponding optoacoustic real time tomographic is sliced (faces YZ) at left figure dotted line.It can be seen that from institute at image described
Optoacoustic, which is popped one's head in, can obtain the high-resolution optoacoustic sectional slice of accurate 7 micron diameter carbon filament with imaging method.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications,
Equivalent substitute mode is should be, is included within the scope of the present invention.
Claims (10)
1. the fast linear cofocus scanning optoacoustic of no water coincidence a kind of is popped one's head in, which is characterized in that including casing, optical fiber collimator,
Galvanometer, flat field scanning lens, hollow two-dimension translational adjustment frame, ultrasonic coupler and ultrasonic transducer；The casing includes
One piece of tabletting and chassis body, the chassis body are equipped with mounting hole, are respectively used to installation galvanometer, optical fiber collimator, flat field and sweep
Retouch lens and hollow two-dimension translational adjustment frame；The ultrasonic coupler and ultrasonic transducer are arranged in hollow two-dimension translational adjustment frame
On；
The ultrasonic coupler includes the top of ultrasonic coupler, the lower part of ultrasonic coupler and the anti-acoustic mirror of light transmission, the ultrasound
The light incident surface and light-emitting face of coupler are horizontally disposed, ensure vertical incidence, the outgoing of light, avoid the deviation of light,
The anti-acoustic mirror of light transmission is obliquely installed from the horizontal by 45 degree, and positioned at the top of ultrasonic coupler, the lower part of ultrasonic coupler
Centre, the top of the ultrasonic coupler, the anti-acoustic mirror of light transmission, ultrasonic coupler lower part closely connect successively；
The ultrasonic transducer is arranged on the lower part of ultrasonic coupler, the flat field scanning lens, hollow after connection
Two-dimension translational adjustment frame, ultrasonic coupler are sequentially coaxially arranged.
2. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 1, which is characterized in that described
Mounting hole is configured as threaded hole；The optical fiber collimator peace field scan lens itself are threaded, and directly pass through casing master
The threaded hole connection to match on body；The galvanometer is fixed on by the porose tabletting of screw holddown belt on chassis body；It is described
The screw connection that chassis body passes through four corners with hollow two-dimension translational adjustment frame.
3. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 2, which is characterized in that described
Optical fiber collimator be it is cylindric, one end carry external screw thread, to on casing threaded hole coordinate fix, the other end be equipped with
The helicitic texture that optical fiber head is of coupled connections.
4. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 1, which is characterized in that described
Galvanometer include smooth coated reflective eyeglass, drive the cylindric motor and signal wire of eyeglass deflection, after the power is turned on, coated reflective
Eyeglass is locked in initial position, and coated reflective eyeglass is arranged with horizontal at 45 degree of angles, the standard being emitted from optical fiber collimator is made
Direct light is through the vertical incident flat field scanning lens of its reflection, after galvanometer is set, using tabletting, is shaken by what screw compression regulated
Mirror, galvanometer control module drive eyeglass to deflect by signal line traffic control motor, the amplitude of the deflection angle of eyeglass and control voltage at
Direct ratio.
5. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 1, which is characterized in that described
Flat field scanning lens possess the function of focus on light beam, make laser in scanning area inner focusing be minimum luminous point, realize high score
Resolution；Flat field scanning lens can also solve the problems, such as when galvanometer wide-angle fan sweeping caused optical focus not in same level,
When this to scan sample surfaces, the focal beam spot size of scanning area periphery and the focal beam spot at center are almost the same, to
Ensure that the lateral resolution in entire scanning area is optimum resolution；Since flat field scanning lens is threadedly secured to machine
On shell, rotary flat field scan lens may be implemented that optical focus is axially adjustable, to realize the confocal excitation of light, sound and detection mould
Formula.
6. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 1, which is characterized in that described
Hollow two-dimension translational adjustment frame in the horizontal plane adjust sound field center and light relative position, realize optoacoustic it is coaxial；
The hollow two-dimension translational adjustment includes hollow two-dimension translational adjustment rack enclosure, adjusting screw rod and putting down with hollow round hole
Part is moved, carrying out the two-dimensional directional on horizontal plane by two adjusting screw rods is adjusted, and is carried on the hollow round hole of translatable part
Screw thread, to connect ultrasonic coupler and ultrasonic transducer.
7. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 1, which is characterized in that described
The top of ultrasonic coupler include solid ultrasonic coupling substance inside the shell and first of ultrasonic coupler upper section；Institute
The top half for stating the shell of ultrasonic coupler upper section is cylinder, carries external thread structure, to flat with hollow two dimension
Move the translatable part connection of adjustment frame, the upper surface of the shell of ultrasonic coupler upper section is horizontal plane, and light is from upper surface
Vertical incidence；The lower half portion of the shell of the ultrasonic coupler upper section be prism, lower surface be one from the horizontal by
The inclined-plane at 45 degree of angles；The axial portions of the shell of ultrasonic coupler upper section are the square three-way groove in axial direction, with straight slot shape
The the first solid ultrasonic coupling substance to match is closely connect with the shell of ultrasonic coupler upper section；
The lower part of the ultrasonic coupler includes the solid ultrasound coupling inside the shell and second of ultrasonic coupler section below
Substance is closed, the shell of the ultrasonic coupler section below is in integrally prism-shaped, and the left side of shell is from the horizontal by 45 degree
The inclined-plane at angle matches with the lower surface on the top of ultrasonic coupler；The shell of ultrasonic coupler section below there are two straight slot,
One is located on the left of the shell of ultrasonic coupler section below, for axial square three-way groove, the straight slot with the top of ultrasonic coupler
Match, the second solid ultrasonic coupling substance and the shell of ultrasonic coupler section below to match with straight slot shape closely connects
It connects；Another runs through straight slot for the rectangular of horizontal direction, for matching connection with ultrasonic transducer.
8. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 7, which is characterized in that described
The anti-acoustic mirror thickness of light transmission be less than 0.1mm, light transmittance 92% or more and surface it is smooth；
The first solid ultrasonic coupling substance and the second solid ultrasonic coupling substance clear, colorless, light transmittance 88% with
On, the light plane of incidence of the first, second solid ultrasonic coupling substance, the face for contacting sample, the face being connect with ultrasonic transducer with
And the face contacted with the anti-acoustic mirror of light transmission is processed by shot blasting, reduces the decaying of light, sound in communication process, other faces are made
Frosting reduces external unrelated light, influence of the sound to experiment.
9. the fast linear cofocus scanning optoacoustic probe of a kind of no water coincidence according to claim 1, which is characterized in that described
Ultrasonic transducer include ultrasonic transducer shell, matching layer, piezoelectric material, backing block and signal wire, the ultrasonic transducer
Shell is prism, and size matches the straight slot in ultrasonic coupler lower horizontal direction, by the one of ultrasonic transducer reception/transmitting ultrasound
The end alignment anti-acoustic mirror of light transmission, it is coaxial with the top of ultrasonic coupler after so that sound field is passed through the anti-acoustic mirror reflection of light transmission, energy converter is connect
Transmit/receive penetrate ultrasound one end closely connect with the solid ultrasonic coupling substance in the axial pass trough of ultrasonic coupler lower part, it is described
The focal length parameter of ultrasonic transducer is set as focal plane and is overlapped with the solid ultrasonic coupling material surface of optoacoustic probe bottom, ultrasound
The electric signal that energy converter obtains passes to computer processing system through signal wire.
10. the imaging side popped one's head according to the fast linear cofocus scanning optoacoustic of any one of the claim 1-9 no water coincidences
Method, which is characterized in that include the following steps：
(1) sample surface to be measured applies a little ultrasonic coupling liquid, is affixed on the solid ultrasonic coupling substance table of optoacoustic probe bottom
Face；
(2) galvanometer powers on, laser emitting module shoot laser；
(3) size of signal is swept according to A, rotary flat field scan lens, ultrasonic coupler and hollow two-dimension translational adjustment frame
Adjusting screw rod realizes that light, sound is confocal and high-resolution imaging to adjust the relative position of optical focus and ultrasonic transducer focal plane,
And optical focus is made to be located just at sample surfaces；
(4) computer processing system gives galvanometer control module and signal acquisition module synchronous triggering signal simultaneously, to carry out light
Line scans and the parallel acquisition of photoacoustic signal, storage；
(5) collected photoacoustic signal is quickly reconstructed reflection sample different structure using GPU to swashing by computer processing system
The photoacoustic image of light difference absorbing state, real time imagery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810527842.0A CN108742528B (en) | 2018-05-29 | 2018-05-29 | Water-free coupling rapid linear confocal scanning photoacoustic probe and imaging method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810527842.0A CN108742528B (en) | 2018-05-29 | 2018-05-29 | Water-free coupling rapid linear confocal scanning photoacoustic probe and imaging method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108742528A true CN108742528A (en) | 2018-11-06 |
| CN108742528B CN108742528B (en) | 2021-12-10 |
Family
ID=64003329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810527842.0A Active CN108742528B (en) | 2018-05-29 | 2018-05-29 | Water-free coupling rapid linear confocal scanning photoacoustic probe and imaging method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108742528B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109620159A (en) * | 2018-12-29 | 2019-04-16 | 深圳先进技术研究院 | A kind of opto-acoustic microscopic imaging device |
| CN111134591A (en) * | 2019-12-27 | 2020-05-12 | 华南师范大学 | Photoacoustic microscopic imaging pen and imaging method |
| CN112353419A (en) * | 2020-11-30 | 2021-02-12 | 简小华 | Multi-array element scanning type ultrasonic probe, ultrasonic imaging system and ultrasonic imaging method |
| WO2021048951A1 (en) * | 2019-09-11 | 2021-03-18 | 日本電信電話株式会社 | Photoacoustic probe |
| CN112773351A (en) * | 2019-11-07 | 2021-05-11 | 华南师范大学 | Thermo-acoustic imaging device and method based on oil-free coupling and impedance matching compact probe |
| CN113029965A (en) * | 2021-03-05 | 2021-06-25 | 华南师范大学 | Photoacoustic/ultrasonic imaging device and method based on spiral type rapid large-range scanning |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054558A (en) * | 2013-01-29 | 2013-04-24 | 广州佰奥廷电子科技有限公司 | Integrated handheld opto-acoustic microscope imaging probe |
| CN105848587A (en) * | 2013-12-25 | 2016-08-10 | 奥林巴斯株式会社 | Photoacoustic microscope |
| CN106333650A (en) * | 2016-09-26 | 2017-01-18 | 华南师范大学 | Multi-scale photo-acoustic micro-imaging device and multi-scale photo-acoustic micro-imaging method |
| CN106769876A (en) * | 2016-11-28 | 2017-05-31 | 电子科技大学 | The new scan method and its device of a kind of optical resolution photoacoustic microscope |
| CN106872367A (en) * | 2017-04-19 | 2017-06-20 | 中国科学院深圳先进技术研究院 | A kind of imaging system and method |
| CN106943120A (en) * | 2017-04-21 | 2017-07-14 | 厦门大学 | A kind of photoacoustic microscope and its method for monitoring microvesicle explosion in biological tissues |
-
2018
- 2018-05-29 CN CN201810527842.0A patent/CN108742528B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054558A (en) * | 2013-01-29 | 2013-04-24 | 广州佰奥廷电子科技有限公司 | Integrated handheld opto-acoustic microscope imaging probe |
| CN105848587A (en) * | 2013-12-25 | 2016-08-10 | 奥林巴斯株式会社 | Photoacoustic microscope |
| CN106333650A (en) * | 2016-09-26 | 2017-01-18 | 华南师范大学 | Multi-scale photo-acoustic micro-imaging device and multi-scale photo-acoustic micro-imaging method |
| CN106769876A (en) * | 2016-11-28 | 2017-05-31 | 电子科技大学 | The new scan method and its device of a kind of optical resolution photoacoustic microscope |
| CN106872367A (en) * | 2017-04-19 | 2017-06-20 | 中国科学院深圳先进技术研究院 | A kind of imaging system and method |
| CN106943120A (en) * | 2017-04-21 | 2017-07-14 | 厦门大学 | A kind of photoacoustic microscope and its method for monitoring microvesicle explosion in biological tissues |
Non-Patent Citations (2)
| Title |
|---|
| HAIGANG MA 等: "Photoacoustic confocal dermoscope with a waterless coupling and impedance matching opto-sono probe", 《OPTICS LETTERS》 * |
| 刘炎炎: "光声显微成像的灵敏度提高研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109620159A (en) * | 2018-12-29 | 2019-04-16 | 深圳先进技术研究院 | A kind of opto-acoustic microscopic imaging device |
| CN109620159B (en) * | 2018-12-29 | 2024-04-02 | 深圳先进技术研究院 | Photoacoustic microscopic imaging device |
| WO2021048951A1 (en) * | 2019-09-11 | 2021-03-18 | 日本電信電話株式会社 | Photoacoustic probe |
| CN112773351A (en) * | 2019-11-07 | 2021-05-11 | 华南师范大学 | Thermo-acoustic imaging device and method based on oil-free coupling and impedance matching compact probe |
| CN112773351B (en) * | 2019-11-07 | 2024-03-22 | 华南师范大学 | Thermo-acoustic imaging device and method based on oil-free coupling and impedance matching compact probe |
| CN111134591A (en) * | 2019-12-27 | 2020-05-12 | 华南师范大学 | Photoacoustic microscopic imaging pen and imaging method |
| CN111134591B (en) * | 2019-12-27 | 2022-09-06 | 华南师范大学 | Photoacoustic microscopic imaging pen and imaging method |
| CN112353419A (en) * | 2020-11-30 | 2021-02-12 | 简小华 | Multi-array element scanning type ultrasonic probe, ultrasonic imaging system and ultrasonic imaging method |
| CN112353419B (en) * | 2020-11-30 | 2024-03-15 | 中国科学院苏州生物医学工程技术研究所 | Multi-array element scanning type ultrasonic probe, ultrasonic imaging system and ultrasonic imaging method |
| CN113029965A (en) * | 2021-03-05 | 2021-06-25 | 华南师范大学 | Photoacoustic/ultrasonic imaging device and method based on spiral type rapid large-range scanning |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108742528B (en) | 2021-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108742528A (en) | A kind of the fast linear cofocus scanning optoacoustic probe and its imaging method of no water coincidence | |
| CN106333650B (en) | A kind of multiple dimensioned opto-acoustic microscopic imaging devices and methods therefor | |
| US10299685B2 (en) | Method and apparatus to enhance light illuminating intensity and diffusivity | |
| CN103637819B (en) | Sound, light are total to the rectum optoacoustic endoscopy lens device of rotation sweep | |
| JP6489797B2 (en) | Subject information acquisition device | |
| US11647957B2 (en) | Ultrasound probe | |
| CN105903667B (en) | The hollow focusing ultrasonic detector of double frequency | |
| WO2011091423A2 (en) | Ultrasonic/photoacoustic imaging devices and methods | |
| CN109620162A (en) | A kind of optoacoustic endoscopy lens device and imaging method based on bessel beam extended focal depth | |
| US20130190594A1 (en) | Scanning Optoacoustic Imaging System with High Resolution and Improved Signal Collection Efficiency | |
| CN104188625A (en) | Multimodal microscopic imaging system | |
| JP2011172730A (en) | Biological information processor | |
| CN103690141A (en) | Internal rectal optical, optoacoustic and ultrasonic multimode imaging endoscope and imaging method thereof | |
| CN105167747A (en) | Handheld photoacoustic imaging probe | |
| CN109199332B (en) | Photoacoustic and ultrasonic bimodal endoscopic imaging device and method based on light reflection | |
| CN109567758A (en) | Across the scale photoacoustic imaging system of one kind | |
| CN204813815U (en) | Hand -held type photoacoustic imaging probe | |
| CN114010151B (en) | Photoacoustic ultrasound multi-mode imaging system | |
| CN112971715B (en) | Imaging device and method for thermo-acoustic, photo-acoustic and ultrasonic collinear excitation and reception | |
| CN111134591B (en) | Photoacoustic microscopic imaging pen and imaging method | |
| CN106821320A (en) | A kind of opto-acoustic microscopic imaging system | |
| CN106419839A (en) | Rapid opto-acoustic/ultrasonic sector-scanning imaging device with large view field and high resolution and method for same | |
| US4208602A (en) | Piezoelectric ultrasonic scanning head using a beryllium mirror | |
| WO2013183247A1 (en) | Acoustooptic imaging device | |
| CN105054895B (en) | For optics, ultrasound, optoacoustic multimode micro-imaging integrated probe |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |