CN108716391B - Method for regulating and controlling endogenous microbial community for oil extraction - Google Patents
Method for regulating and controlling endogenous microbial community for oil extraction Download PDFInfo
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- 238000000605 extraction Methods 0.000 title claims description 8
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- 238000012360 testing method Methods 0.000 claims abstract description 114
- 238000002347 injection Methods 0.000 claims abstract description 100
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- 230000008569 process Effects 0.000 claims abstract description 17
- 238000011084 recovery Methods 0.000 claims abstract description 17
- 230000000694 effects Effects 0.000 claims abstract description 16
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- 239000003921 oil Substances 0.000 claims description 149
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- 235000019698 starch Nutrition 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
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- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 5
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 5
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 239000003129 oil well Substances 0.000 claims description 5
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- 241000159512 Geotrichum Species 0.000 claims 1
- 241001467578 Microbacterium Species 0.000 claims 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims 1
- 235000019797 dipotassium phosphate Nutrition 0.000 claims 1
- 229910000397 disodium phosphate Inorganic materials 0.000 claims 1
- 235000019800 disodium phosphate Nutrition 0.000 claims 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims 1
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Classifications
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B49/00—Testing the nature of borehole walls; Formation testing; Methods or apparatus for obtaining samples of soil or well fluids, specially adapted to earth drilling or wells
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Abstract
The invention belongs to the technical field of tertiary oil recovery, and particularly relates to a method for regulating and controlling an endogenous microbial community for oil recovery. The method comprises the steps of firstly screening a test oil reservoir, determining simulated core parameters according to the conditions of the test oil reservoir, further developing a physical simulation experiment of the test oil reservoir, determining a microbial community distribution rule according to the physical simulation experiment result of the test oil reservoir, so as to determine the injection amount and the field injection process of different types of microbial activators, and finally performing a field test. The invention can effectively activate different types of microorganisms in the test oil reservoir, and simultaneously effectively reduce the investment cost of the microorganism activator, thereby greatly improving the recovery ratio of the test oil reservoir, having obvious field test effect, having an input-output ratio of more than 1:12, and improving the recovery ratio by more than 20%.
Description
Technical Field
The invention belongs to the technical field of tertiary oil recovery, and particularly relates to a method for regulating and controlling an endogenous microbial community for oil recovery.
Background
The endogenous microbial oil displacement refers to the microbial community formed in the long-term water injection process of an oil reservoir, the microbial community in the oil reservoir is activated by injecting an activating agent into a water injection well, and the yield and the recovery ratio of crude oil are improved by utilizing the action of surface active substances and biogas generated by the microorganisms and metabolism and the crude oil in the oil reservoir, so that the method is an environment-friendly and low-cost exploitation technology.
The oil displacement method is characterized in that aerobic microorganisms, facultative aerobic microorganisms and anaerobic microorganisms are sequentially distributed in an oil reservoir from a water injection well to a production well, different types of microorganisms penetrate through the whole oil reservoir, and different types of microorganisms and metabolites thereof jointly act with crude oil in the oil reservoir, so that the oil displacement effect is generated. However, although different types of microbial activators are injected from the water injection well to activate aerobic microorganisms, facultative aerobic microorganisms and anaerobic microorganisms in the current endogenous microbial oil displacement process, the different types of activators are all injected from the water injection well into the oil reservoir, so that on one hand, the activators have adsorption retention effects, and on the other hand, the activators of different types overlap to influence the effective activation effects of the microorganisms. Therefore, the distribution rule of different types of microorganisms in the oil reservoir needs to be determined, so that the accurate injection of different types of microorganism activators is realized, and the effective activation is realized.
Through the literature retrieval, the publication number "CN 104481476A", the patent name "a method for improving the recovery ratio of high oil by microbial oil displacement", disclose a method for improving the recovery ratio of high oil by microbial oil displacement, through detecting the species of endogenous microbes, divide the microbial oil displacement into different stages of aerobic, facultative and anaerobic microbial oil displacement. However, this method has disadvantages in that: (1) different types of microorganisms including aerobic, facultative aerobic and anaerobic microorganisms exist in the oil deposit from the water injection well to the production well at the same time, and only one type of microorganism can be activated to drive oil; (2) the different microbial oil displacement stages in the oil reservoir are injected by water injection wells, the difference between the injection speed and the injection mode is avoided, and the activator is mainly consumed and utilized at the front part in the oil reservoir and is not beneficial to the activation of deep microbes in the oil reservoir; (3) the method also needs to inject bacterial liquids of different types of microorganisms, needs to screen, culture and ferment the microorganisms, has complex and tedious implementation process, is not beneficial to field implementation, and also has the problem of oil deposit adaptability.
Disclosure of Invention
The present invention aims to provide a method for controlling the endogenous microbial community for oil production, aiming at the defects of the prior art. The method comprises the steps of firstly screening a test oil reservoir, determining simulated core parameters according to the conditions of the test oil reservoir, further developing a physical simulation experiment of the test oil reservoir, determining a microbial community distribution rule according to the physical simulation experiment result of the test oil reservoir, so as to determine the injection amount and the field injection process of different types of microbial activators, and finally performing a field test. The invention can effectively activate different types of microorganisms in the test oil reservoir, and simultaneously effectively reduce the investment cost of the microorganism activator, thereby greatly improving the recovery ratio of the test oil reservoir and having obvious field test effect.
The invention discloses a method for regulating and controlling an endogenous microbial community for oil extraction, which is characterized by comprising the following steps, but not limited to the following steps:
(1) screening of test reservoirs
The screening of the test reservoir needs to meet the following conditions: the oil deposit temperature is less than 90 ℃, the oil deposit pressure is less than 20MPa, and the permeability is more than 100 multiplied by 10-3μm2The oil reservoir stratum water mineralization degree is less than 200000mg/L and the oil reservoir crude oil viscosity is less than 50000 mPa.
(2) Determination of experimental simulated core parameters
The parameters of the experimental simulated rock core comprise: core length, diameter and sampling point;
experiment simulation core length: the ratio of the distance between the oil-water wells of the test oil reservoir to the length of the test simulation core is 100-;
experiment simulation core diameter: the ratio of the thickness of the test oil reservoir to the diameter of the test simulated core is 50-80: 1;
experiment simulation core sampling point: the number of sampling points is that one sampling point is arranged at intervals of 0.1m-0.2m, and the sampling points are uniformly distributed on the experimental simulated core.
(3) Physical simulation experiment for testing oil reservoir
The physical simulation experiment of the test oil reservoir is carried out by utilizing the experiment simulation rock core, and the experiment comprises the following specific steps:
filling an experimental simulated rock core with the same permeability as the experimental oil reservoir; simulating formation water of a core vacuumizing and saturation test oil reservoir; dehydrating and degassing crude oil of a saturation test reservoir; placing the experimental simulation core for 10 days under the conditions of temperature and pressure of the experimental oil reservoir, and then performing primary water flooding until the water content of the experimental simulation core is consistent with the current comprehensive water content of the experimental oil reservoir; sampling at sampling points of the experimental simulated rock core, wherein the sampling amount of each sampling point is 10-20 mL; the samples were tested for aerobic, facultative aerobic and anaerobic microorganism content.
(4) Determination of microbial community distribution rule in experimental simulation rock core
According to the test results, the distribution rules of aerobic, facultative aerobic and anaerobic microorganisms in the rock core are analyzed and simulated by the experiment, and the boundary points of the aerobic and facultative aerobic, facultative aerobic and anaerobic microorganisms are determined.
(5) Determination of microbial community distribution rule in experimental oil reservoir
Respectively determining the distance L between the aerobic and facultative aerobic boundary points in the test oil deposit and the water injection well according to the determined boundary points of the aerobic and facultative aerobic and anaerobic microorganisms and the proportion of the distance between the oil-water wells of the test oil deposit and the length of the experimental simulated rock core1Distance L between the boundary point of facultative aerobic and anaerobic microorganisms and water injection well2。
(6) Determination of the amount of activator injected
The activator includes an aerobic microorganism activator, a facultative aerobic microorganism activator, and an anaerobic microorganism activator.
(7) Determination of activator in-situ injection process
And the site activator injection process is that according to the positions corresponding to the boundary points of the aerobic and facultative aerobic and anaerobic microorganisms in the test oil reservoir determined by the steps, the facultative aerobic and anaerobic microorganism activators are respectively arranged at the positions, the facultative aerobic and anaerobic microorganism activators are respectively injected from the injection ports, and the aerobic microorganism activators are injected from the water injection wells of the test oil reservoir.
(8) On-site test and evaluation of Effect
And (4) performing a field test according to the determined injection amount of the activating agent and the field injection process of the activating agent, counting and analyzing the field test effect after the test is finished, and calculating the improved recovery rate value and the input-output ratio of the test oil reservoir.
The aerobic microorganisms comprise bacillus subtilis, pseudomonas aeruginosa and acinetobacter.
The facultative aerobes include Geobacillus, oleaginous bacillus and Achromobacter.
The anaerobic microorganisms comprise methanogens, nitrate reducing bacteria and anaerobic bacilli.
The aerobic microorganism activator comprises 1.5-2.0 wt% of starch, 0.9-1.2 wt% of peptone, 0.3-0.6 wt% of dipotassium hydrogen phosphate and 1.0-1.5 wt% of hydrogen peroxide.
The facultative aerobe activator is glucose 1.8-2.2 wt%, corn steep liquor dry powder 1.0-1.4 wt%, disodium hydrogen phosphate 0.4-0.8 wt%.
The anaerobic microorganism activator comprises 1.5-2.0 wt% of starch, 0.5-0.8 wt% of corn steep liquor dry powder and 0.3-0.6 wt% of diammonium hydrogen phosphate.
The injection amount of the aerobic microorganism activator is determined as follows:
V1＝R2L1Фβ1
in the formula: v1Amount of aerobic microbial activator injected, m3；
R-test reservoir thickness, m;
L1the distance between the boundary point of the aerobic and facultative aerobic and the water injection well, m;
phi is the porosity of the tested oil reservoir and has no dimension;
β1the dosage coefficient is dimensionless and the value range is 0.5-0.6.
The injection amount of the facultative aerobe activator is determined as follows:
V2＝R2(L2-L1)Фβ2
in the formula: v2Amount of facultative aerobic microbial activator injected, m3；
R-test reservoir thickness, m;
L1the distance between the boundary point of the aerobic and facultative aerobic and the water injection well, m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well, m;
phi is the oil reservoir porosity, and is dimensionless;
β2the dosage coefficient is dimensionless and the value range is 0.8-1.0.
The determination of the amount of the anaerobic microorganism activator injected is as follows:
V3＝R2(L-L2)Фβ3
in the formula: v3Amount of anaerobic microorganism activator injected, m3；
R-test reservoir thickness, m;
l is the distance between a test oil reservoir water injection well and an oil well, m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well, m;
phi is the oil reservoir porosity, and is dimensionless;
β3the dosage coefficient is dimensionless and the value range is 0.6-0.8.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the distribution areas of aerobic, facultative aerobic and anaerobic microorganisms at different positions in a test oil reservoir are determined by using an experimental simulation core through a simulation experiment, and a basis is provided for the design of the injection amount of the activating agent;
(2) injecting activators of different types of microorganisms from different positions of a test oil reservoir to improve the pertinence and the effectiveness of the activators for activating the microorganisms, so that the oil displacement effect of the microorganisms is improved, the input-output ratio is more than 1:12, and the recovery ratio is improved by more than 20%;
(3) the activator system carries out directional activation according to the distribution area of microorganisms in a test oil reservoir, thereby effectively avoiding the adsorption and retention of activator components and saving the cost of the activator;
(4) the method has strong pertinence and operability in field implementation, and has the advantages of low mining cost, no need of subsequent treatment of produced liquid, safety, environmental protection and the like compared with the traditional method.
Detailed Description
The invention will be further described in detail with reference to the following specific examples:
example 1:
summary of test block a for a certain production plant in the victory oil field: the oil reservoir temperature is 65 ℃, the oil reservoir pressure is 11MPa, the oil layer thickness is 6.5m, and the permeability is 800 multiplied by 10-3μm2The salinity of the formation water is 8500mg/L, the porosity is 26%, the viscosity of the crude oil is 1200mPa & s, the comprehensive water content is 93.5%, the production degree is 24.0%, and the average well spacing is 240 m. The method for carrying out microbial oil displacement on the oil reservoir comprises the following specific steps:
(1) screening of test reservoirs
The oil reservoir temperature is 65 ℃, the oil reservoir pressure is 11MPa, and the oil reservoir permeability is 800 multiplied by 10-3μm2The formation water mineralization degree is 8500mg/L, and the crude oil viscosity is 1200 mPas. Meets the screening criteria of the test reservoir of the present invention and thus can be practiced.
(2) Determination of experimental simulated core parameters
Experiment simulation core length: the distance between the oil-water wells is 240m, the ratio of the distance between the oil-water wells of the tested oil reservoir to the length of the experimental simulated core is 100:1, and the length of the core is calculated to be 2.4 m.
Experiment simulation core diameter: the thickness of the oil reservoir is 6.5m, the ratio of the thickness of the experimental oil reservoir to the diameter of the experimental simulated core is 50:1, and the diameter of the core is calculated to be 0.13 m.
Experiment simulation core sampling point: the core length is 2.4m, and the sampling point interval is 0.2m, and the number of designed sampling points is 12.
(3) Physical simulation experiment for testing oil reservoir
The physical simulation experiment of the test oil reservoir is carried out by utilizing the experiment simulation rock core, and the experiment comprises the following specific steps: filling rock core according to reservoir A condition, wherein the rock core basically requires permeability of 800 multiplied by 10-3μm2The porosity is 26%, the reservoir formation water is saturated, the reservoir is saturated, the crude oil is dehydrated and degassed, and the core is aged and placed for 10 days by simulating the reservoir temperature; secondly, performing primary water drive on the rock core, wherein the extraction degree of the primary water drive is 24 percent, and the produced liquid contains 93.5 percent of water; thirdly, sampling 12 sampling points of the rock core after one water flooding for carrying out microbial analysis, wherein the specific data are shown in the following table 1:
TABLE 1 concentration Change of different types of microorganisms in the output fluid at core sampling points
(4) Determination of microbial community distribution rule in experimental simulation rock core
According to the test results, the analysis results of the core displacement experiment show that the aerobic microorganisms are mainly distributed in the area between the No. 1 sampling point and the No. 3 sampling point of the core, the facultative aerobic microorganisms are mainly distributed in the area between the No. 4 sampling point and the No. 7 sampling point of the core, and the anaerobic microorganisms are mainly distributed in the area between the No. 8 sampling point and the No. 12 sampling point of the core. Therefore, the position of the boundary point of the aerobic and facultative aerobic microorganisms at a distance of 0.6m from the core inlet and the position of the boundary point of the facultative aerobic and anaerobic microorganisms at a distance of 1.4m from the core inlet are determined according to the distribution rule of microorganisms in different areas.
(5) Determination of microbial community distribution rule in experimental oil reservoir
According to the boundary points of the aerobic and facultative aerobic and anaerobic microorganisms determined in the steps and the ratio of the distance between the oil-water wells of the tested oil deposit to the length of the experimental simulated rock core of 100:1, the distance L between the boundary points of the aerobic and facultative aerobic in the tested oil deposit and the water injection well is determined160m, and the distance L between the boundary point of facultative aerobic and anaerobic microorganisms and the water injection well2Is 140 m.
(6) Determination of the amount of activator injected
The activator includes an aerobic microorganism activator, a facultative aerobic microorganism activator, and an anaerobic microorganism activator. Wherein the aerobic microorganism activator is: 1.5 wt% of starch, 1 wt% of peptone, 0.3 wt% of dipotassium hydrogen phosphate and 1.5 wt% of hydrogen peroxide, and the injection amount of the aerobic microorganism activator is determined as follows:
V1＝3.14R2L1Фβ1＝1035m3
in the formula: v1Amount of aerobic microbial activator injected, m3；
R-test reservoir thickness 6.5 m;
L1the distance between the injection well and the boundary point of aerobic and facultative aerobic microorganisms is 60 m;
phi-reservoir porosity 0.26;
β1the dose factor, dimensionless, is 0.5.
The facultative aerobe activator is glucose 2.2 wt%, corn steep liquor dry powder 1.2 wt%, disodium hydrogen phosphate 0.6 wt%, and the injection amount of the facultative aerobe activator is determined as follows:
V2＝3.14R2(L2-L1)Фβ2＝2483m3
in the formula: v2Amount of facultative aerobic microbial activator injected, m3；
R-test reservoir thickness 6.5 m;
L1distance between boundary point of aerobic and facultative aerobic and water injection well60m；
L2The distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well is 140 m;
phi-reservoir porosity 0.26;
β2the dose factor, dimensionless, is 0.9.
The anaerobic microorganism activator comprises 1.6 wt% of starch, 0.7 wt% of corn steep liquor dry powder and 0.5 wt% of diammonium hydrogen phosphate, and the injection amount of the anaerobic microorganism activator is determined as follows:
V3＝3.14R2(L-L2)Фβ3＝2424.5m3
in the formula: v3Amount of anaerobic microorganism activator injected, m3；
R-test reservoir thickness 6.5 m;
l is the distance between a water injection well of the test oil reservoir and an oil well is 240 m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well is 140 m;
phi-reservoir porosity 0.26;
β3the dose factor, dimensionless, is 0.7.
(7) Determination of activator injection process
The site activator injection process is that according to the determined positions of the boundary points of aerobic and facultative aerobic and anaerobic microorganisms in the test oil reservoir, the injection ports of facultative aerobic and anaerobic microorganism activators are respectively arranged, wherein the aerobic microorganism activators are injected from the water injection well of the test oil reservoir, the facultative aerobic microorganism activators are injected from the injection ports 60m away from the water injection well, and the anaerobic microorganism activators are injected from the injection ports 140m away from the water injection well.
(8) On-site test and evaluation of Effect
After the field test is finished, the comprehensive water content of the block is reduced to 76.5% from 93.5%, the water content is reduced by 17%, and the crude oil yield is increased by 2.5 multiplied by 104t, the recovery ratio is improved by 21.8 percent, the input-output ratio is 1:10.5, and the field test effect is good.
Example 2
Summary of test block B for a certain production plant in the victory oil field: the oil reservoir temperature is 80 ℃, the oil reservoir pressure is 15MPa, the oil layer thickness is 4m, and the permeability is 1200 multiplied by 10-3μm2The salinity of formation water is 10500mg/L, the porosity is 28%, the viscosity of crude oil is 850mPa · s, the comprehensive water content is 94.8%, the production degree is 30%, and the average well spacing is 200 m. The method for carrying out endogenous microbial oil displacement on the oil reservoir comprises the following specific steps:
(1) screening of test reservoirs
The oil reservoir temperature is 80 ℃, the oil reservoir pressure is 15MPa, and the oil reservoir permeability is 1200 multiplied by 10-3μm2The formation water mineralization degree is 10500mg/L, and the crude oil viscosity is 850 mPas. Meets the screening criteria of the test reservoir of the present invention and thus can be practiced.
(2) Determination of experimental simulated core parameters
Experiment simulation core length: the distance between the oil-water wells is 200m, the ratio of the distance between the oil-water wells of the tested oil reservoir to the length of the experimental simulated core is 200:1, and the length of the core is calculated to be 1 m.
Experiment simulation core diameter: the thickness of the oil reservoir is 4m, the ratio of the thickness of the experimental oil reservoir to the diameter of the experimental simulated core is 50:1, and the diameter of the core is calculated to be 0.08 m.
Experiment simulation core sampling point: the core length is 2m, and the sampling point interval is 0.1m, and 10 sampling points are calculated.
(3) Physical simulation experiment for testing oil reservoir
The physical simulation experiment of the test oil reservoir is carried out by utilizing the experiment simulation rock core, and the experiment comprises the following specific steps: filling rock core according to reservoir B condition, wherein the rock core basically requires permeability of 1200 multiplied by 10-3μm2The porosity is 28%, the reservoir formation water is saturated, the reservoir is saturated, the crude oil is dehydrated and degassed, and the core simulation reservoir temperature is kept for 10 days; secondly, performing primary water drive on the rock core, wherein the extraction degree of the primary water drive is 30 percent, and the produced liquid contains 94.8 percent of water; thirdly, sampling 10 sampling points of the rock core after one water flooding for carrying out microbial analysis, wherein the specific data are shown in the following table 2:
TABLE 2 concentration changes of different types of microorganisms in the output fluid at core sampling points
(4) Determination of microbial community distribution rule in experimental simulation rock core
According to the test results, the analysis results of the core displacement experiment show that the aerobic microorganisms are mainly distributed in the area between the No. 1 sampling point and the No. 3 sampling point of the core, the facultative aerobic microorganisms are mainly distributed in the area between the No. 4 sampling point and the No. 6 sampling point of the core, and the anaerobic microorganisms are mainly distributed in the area between the No. 7 sampling point and the No. 10 sampling point of the core. Therefore, the position of the boundary point of the aerobic and facultative aerobic microorganisms at a distance of 0.3m from the inlet of the rock core and the position of the boundary point of the facultative aerobic and anaerobic microorganisms at a distance of 0.6m from the inlet of the rock core are determined according to the distribution rule of microorganisms in different areas.
(5) Determination of microbial community distribution rule in experimental oil reservoir
According to the boundary points of the aerobic and facultative aerobic and anaerobic microorganisms determined in the steps and the proportion of the distance between the oil-water wells of the tested oil reservoir and the length of the experimental simulated rock core, the distance L between the boundary points of the aerobic and facultative aerobic and the water injection well in the tested oil reservoir is determined160m, and the distance L between the boundary point of facultative aerobic and anaerobic microorganisms and the water injection well2Is 120 m.
(6) Determination of the amount of activator injected
The activator includes an aerobic microorganism activator, a facultative aerobic microorganism activator, and an anaerobic microorganism activator. Wherein the aerobic microorganism activator is: 1.7 wt% of starch, 1.1 wt% of peptone, 0.4 wt% of dipotassium hydrogen phosphate and 1.3 wt% of hydrogen peroxide, and the injection amount of the aerobic microorganism activator is determined as follows:
V1＝3.14R2L1Фβ1＝506m3
in the formula: v1Amount of aerobic microbial activator injected, m3；
R-test reservoir thickness 4 m;
L1injection into the well to aerobic andthe distance between the dividing points of the facultative aerobes is 60 m;
phi-reservoir porosity 0.28;
β1the dose factor, dimensionless, is 0.6.
The facultative aerobe activator is glucose 1.9 wt%, corn steep liquor dry powder 1.3 wt%, disodium hydrogen phosphate 0.5 wt%, and the injection amount of the facultative aerobe activator is determined as follows:
V2＝3.14R2(L2-L1)Фβ2＝759m3
in the formula: v2Amount of facultative aerobic microbial activator injected, m3；
R-test reservoir thickness 4 m;
L1the distance between the boundary point of the aerobic and facultative aerobic and the water injection well is 60 m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well is 120 m;
phi-reservoir porosity 0.28;
β2the dose factor, dimensionless, is 0.9.
The anaerobic microorganism activator comprises 1.8 wt% of starch, 0.8 wt% of corn steep liquor dry powder and 0.6 wt% of diammonium hydrogen phosphate, and the injection amount of the anaerobic microorganism activator is determined as follows:
V3＝3.14R2(L-L2)Фβ3＝900m3
in the formula: v3Amount of anaerobic microorganism activator injected, m3；
R-test reservoir thickness 4 m;
l-the distance between a test oil reservoir water injection well and an oil well is 200 m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well is 120 m;
phi-reservoir porosity 0.28;
β3the dose coefficient, dimensionless, is 0.8.
(7) Determination of activator injection process
The site activator injection process is that according to the determined positions of the boundary points of the aerobic and facultative aerobic and anaerobic microorganisms in the test oil reservoir, the injection ports of the facultative aerobic and anaerobic microorganism activators are respectively arranged, wherein the aerobic microorganism activators are injected from the water injection well of the test oil reservoir, the facultative aerobic microorganism activators are injected from the injection ports 60m away from the water injection well, and the anaerobic microorganism activators are injected from the injection ports 120m away from the water injection well.
(8) Statistics and analysis of field test effects
After the field test is finished, the comprehensive water content of the block is reduced from 94.8 percent to 84.5 percent, the water content is reduced by 10.3 percent, and the yield of the crude oil is increased by 3.2 multiplied by 104t, the recovery ratio is increased by 22.5 percent, the input-output ratio is 1:12.0, and the field test effect is good.
Example 3:
summary of test block C for a certain production plant in the victory oil field: the oil reservoir temperature is 75 ℃, the oil reservoir pressure is 12.5MPa, the oil layer thickness is 10m, and the permeability is 1050 multiplied by 10-3μm2The mineralization degree of formation water is 9400mg/L, the porosity is 32%, the viscosity of crude oil is 690mPa & s, the comprehensive water content is 89%, the extraction degree is 24%, and the average well spacing is 300 m. The method for developing the endogenous microbial oil displacement test on the oil reservoir comprises the following specific steps:
(1) screening of test reservoirs
The oil reservoir temperature is 75 ℃, the oil reservoir pressure is 12.5MPa, and the oil reservoir permeability is 1050 multiplied by 10-3μm2The formation water salinity was 9400mg/L, and the crude oil viscosity was 690 mPas. Meets the screening criteria of the test reservoir of the present invention and thus can be practiced.
(2) Determination of experimental simulated core parameters
Experiment simulation core length: the distance between the oil-water wells is 300m, the ratio of the distance between the oil-water wells of the tested oil reservoir to the length of the experimental simulated core is 100:1, and the length of the core is calculated to be 3 m.
Experiment simulation core diameter: the thickness of the oil reservoir is 10m, the ratio of the thickness of the experimental oil reservoir to the diameter of the experimental simulated core is 80:1, and the diameter of the core is calculated to be 0.125 m.
Experiment simulation core sampling point: the core length is 3m, and the sampling point interval is 0.2m, and the number of the calculated sampling points is 15.
(3) Physical simulation experiment for testing oil reservoir
The physical simulation experiment of the test oil reservoir is carried out by utilizing the experiment simulation rock core, and the experiment comprises the following specific steps: filling rock core according to reservoir C condition, the rock core basically requires permeability 1050X 10-3μm2The porosity is 32%, the formation water of the saturated oil reservoir, the dehydrated and degassed crude oil of the saturated oil reservoir and the temperature of the core simulated oil reservoir are kept for 10 days; secondly, performing primary water drive on the rock core, wherein the extraction degree of the primary water drive is 24 percent, and the produced liquid contains 89 percent of water; thirdly, sampling 15 sampling points of the rock core after one water flooding for carrying out microbial analysis, wherein the specific data are shown in the following table 3:
TABLE 3 concentration Change of different types of microorganisms in the output fluid at core sampling points
(4) Determination of microbial community distribution rule in experimental simulation rock core
According to the test results, the analysis results of the core displacement experiment show that the aerobic microorganisms are mainly distributed in the area between the No. 1 sampling point and the No. 5 sampling point of the core, the facultative aerobic microorganisms are mainly distributed in the area between the No. 6 sampling point and the No. 11 sampling point of the core, and the anaerobic microorganisms are mainly distributed in the area between the No. 12 sampling point and the No. 15 sampling point of the core. Therefore, the position of the boundary point of aerobic and facultative aerobic microorganisms at a distance of 1m from the core inlet and the position of the boundary point of facultative aerobic and anaerobic microorganisms at a distance of 2.2m from the core inlet are determined according to the distribution rule of microorganisms in different areas.
(5) Determination of microbial community distribution rule in experimental oil reservoir
According to the boundary points of the aerobic and facultative aerobic and anaerobic microorganisms determined in the steps and the proportion of the distance between the oil-water wells of the tested oil reservoir and the length of the experimental simulated rock core, the distance L between the boundary points of the aerobic and facultative aerobic and the water injection well in the tested oil reservoir is determined1100m, the distance L between the boundary point of facultative aerobic and anaerobic microorganisms and the water injection well2Is 220 m.
(6) Determination of the amount of activator injected
The activator includes an aerobic microorganism activator, a facultative aerobic microorganism activator, and an anaerobic microorganism activator. Wherein the aerobic microorganism activator is: 1.8 wt% of starch, 0.9 wt% of peptone, 0.5 wt% of dipotassium hydrogen phosphate and 1.5 wt% of hydrogen peroxide, and the injection amount of the aerobic microorganism activator is determined as follows:
V1＝3.14R2L1Фβ1＝5024m3
in the formula: v1Amount of aerobic microbial activator injected, m3；
R-test reservoir thickness 10 m;
L1the distance between the injection well and the boundary point of aerobic and facultative aerobic microorganisms is 100 m;
phi-reservoir porosity 0.32;
β1the dose factor, dimensionless, is 0.5.
The facultative aerobe activator is 2 wt% of glucose, 1.3 wt% of corn steep liquor dry powder and 0.5 wt% of disodium hydrogen phosphate, and the injection amount of the facultative aerobe activator is determined as follows:
V2＝3.14R2(L2-L1)Фβ2＝9646m3
in the formula: v2Amount of facultative aerobic microbial activator injected, m3；
R-test reservoir thickness 10 m;
L1the distance between the boundary point of the aerobic and facultative aerobic and the water injection well is 100 m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well is 220 m;
phi-reservoir porosity 0.32;
β2the dose coefficient, dimensionless, is 0.8.
The anaerobic microorganism activator comprises 1.5 wt% of starch, 0.6 wt% of corn steep liquor dry powder and 0.4 wt% of diammonium hydrogen phosphate, and the injection amount of the anaerobic microorganism activator is determined as follows:
V3＝3.14R2(L-L2)Фβ3＝4823m3
in the formula: v3Amount of anaerobic microorganism activator injected, m3；
R-test reservoir thickness 10 m;
l-the distance between a water injection well of the test oil reservoir and an oil well is 300 m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well is 220 m;
phi-reservoir porosity 0.32;
β3the dose factor, dimensionless, is 0.6.
(7) Determination of activator injection process
The site activator injection process is that according to the determined positions of the boundary points of the aerobic and facultative aerobic and anaerobic microorganisms in the test oil reservoir, the injection ports of the facultative aerobic and anaerobic microorganism activators are respectively arranged, wherein the aerobic microorganism activators are injected from the water injection well of the test oil reservoir, the facultative aerobic microorganism activators are injected from the injection ports 100m away from the water injection well, and the anaerobic microorganism activators are injected from the injection ports 220m away from the water injection well.
(8) Statistics and analysis of field test effects
After the field test is finished, the comprehensive water content of the block is reduced to 74.5 percent from 89 percent, the water content is reduced by 14.5 percent, and the crude oil yield is increased by 5.59 multiplied by 104t, the recovery ratio is improved by 21.7 percent, the input-output ratio is 1:13.2, and the field test effect is good.
Claims (3)
1. The method for regulating and controlling the endogenous microbial community for oil extraction is characterized by comprising the following steps:
(1) screening of test reservoirs
The screening of the test reservoir needs to meet the following conditions: the oil deposit temperature is less than 90 ℃, the oil deposit pressure is less than 20MPa, and the permeability is more than 100 multiplied by 10-3μm2The water mineralization degree of the oil reservoir stratum is less than 200000mg/L and the viscosity of the oil reservoir crude oil is less than 50000mPa & s;
(2) determination of experimental simulated core parameters
The parameters of the experimental simulated rock core comprise: core length, diameter and sampling point;
experiment simulation core length: the ratio of the distance between the oil-water wells of the test oil reservoir to the length of the test simulation core is 100-; experiment simulation core diameter: the ratio of the thickness of the test oil reservoir to the diameter of the test simulated core is 50-80: 1; experiment simulation core sampling point: the number of sampling points is that one sampling point is arranged at intervals of 0.1m-0.2m, and the sampling points are uniformly distributed on the experimental simulation core;
(3) physical simulation experiment for testing oil reservoir
The physical simulation experiment of the test oil reservoir is carried out by utilizing the experiment simulation rock core, and the experiment comprises the following specific steps: filling an experimental simulated rock core with the same permeability as the experimental oil reservoir; simulating formation water of a core vacuumizing and saturation test oil reservoir; dehydrating and degassing crude oil of a saturation test reservoir; placing the experimental simulation core for 10 days under the conditions of temperature and pressure of the experimental oil reservoir, and then performing primary water flooding until the water content of the experimental simulation core is consistent with the current comprehensive water content of the experimental oil reservoir; sampling at sampling points of the experimental simulated rock core, wherein the sampling amount of each sampling point is 10-20 mL; testing the aerobic, facultative aerobic and anaerobic microorganism contents of the sample;
(4) determination of microbial community distribution rule in experimental simulation rock core
According to the test result, analyzing the distribution rule of aerobic, facultative aerobic and anaerobic microorganisms in the experimental simulation core, and determining the boundary points of the aerobic and facultative aerobic, facultative aerobic and anaerobic microorganisms;
(5) determination of microbial community distribution rule in experimental oil reservoir
Respectively determining the distance L between the aerobic and facultative aerobic boundary points in the test oil deposit and the water injection well according to the determined boundary points of the aerobic and facultative aerobic and anaerobic microorganisms and the proportion of the distance between the oil-water wells of the test oil deposit and the length of the experimental simulated rock core1The boundary point of facultative aerobic and anaerobic microorganisms and the water injection wellDistance L2；
(6) Determination of the amount of activator injected
The activator comprises an aerobic microorganism activator, a facultative aerobic microorganism activator and an anaerobic microorganism activator;
(7) determination of activator in-situ injection process
The on-site activator injection process is characterized in that according to the positions corresponding to the boundary points of aerobic and facultative aerobic and anaerobic microorganisms in the test oil reservoir determined in the step, the positions are respectively provided with an injection port for the facultative aerobic and anaerobic microorganism activators, the facultative aerobic and anaerobic microorganism activators are respectively injected from the injection ports, and the aerobic microorganism activators are injected from a water injection well of the test oil reservoir;
(8) on-site test and evaluation of Effect
Performing a field test according to the determined injection amount of the activator and the field injection process of the activator, counting and analyzing the field test effect after the test is finished, and calculating the improved recovery rate value and the input-output ratio of the test oil reservoir;
the injection amount of the aerobic microorganism activator is determined as follows:
V1＝R2L1Фβ1
in the formula: v1Amount of aerobic microbial activator injected, m3；
R-test reservoir thickness, m;
L1the distance between the boundary point of the aerobic and facultative aerobic and the water injection well, m;
phi is the porosity of the tested oil reservoir and has no dimension;
β1the dosage coefficient is dimensionless and has a value range of 0.5-0.6;
the injection amount of the facultative aerobe activator is determined as follows:
V2＝R2(L2-L1)Фβ2
in the formula: v2Amount of facultative aerobic microbial activator injected, m3；
R-test reservoir thickness, m;
L1the distance between the boundary point of the aerobic and facultative aerobic and the water injection well, m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well, m;
phi is the oil reservoir porosity, and is dimensionless;
β2the dosage coefficient is dimensionless and has a value range of 0.8-1.0;
the determination of the amount of the anaerobic microorganism activator injected is as follows:
V3＝R2(L-L2)Фβ3
in the formula: v3Amount of anaerobic microorganism activator injected, m3；
R-test reservoir thickness, m;
l is the distance between a test oil reservoir water injection well and an oil well, m;
L2the distance between the boundary point of the facultative aerobic microorganisms and the anaerobic microorganisms and the water injection well, m;
phi is the oil reservoir porosity, and is dimensionless;
β3the dosage coefficient is dimensionless and the value range is 0.6-0.8.
2. The method of claim 1, wherein the aerobic microorganisms comprise bacillus subtilis, pseudomonas aeruginosa and acinetobacter, the facultative aerobic microorganisms comprise bacillus geotrichum, agrobacterium tumefaciens and achromobacter, and the anaerobic microorganisms comprise methanogens, nitrate-reducing bacteria and microbacterium anaerobacter.
3. The method for controlling the endogenous microbial community for oil recovery according to claim 1 or 2, wherein the aerobic microbial activator comprises 1.5-2.0 wt% of starch, 0.9-1.2 wt% of peptone, 0.3-0.6 wt% of dipotassium phosphate, 1.0-1.5 wt% of hydrogen peroxide, 1.8-2.2 wt% of glucose as a facultative aerobic microbial activator, 1.0-1.4 wt% of corn steep liquor dry powder, 0.4-0.8 wt% of disodium phosphate, 1.5-2.0 wt% of starch as an anaerobic microbial activator, 0.5-0.8 wt% of corn steep liquor dry powder and 0.3-0.6 wt% of diammonium phosphate.
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