CN108700522B - Method, device, chip and detection equipment for detecting substance - Google Patents
Method, device, chip and detection equipment for detecting substance Download PDFInfo
- Publication number
- CN108700522B CN108700522B CN201780002409.9A CN201780002409A CN108700522B CN 108700522 B CN108700522 B CN 108700522B CN 201780002409 A CN201780002409 A CN 201780002409A CN 108700522 B CN108700522 B CN 108700522B
- Authority
- CN
- China
- Prior art keywords
- chip
- partition
- detected
- detection
- laser source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Abstract
The application discloses a method, a device, a chip and a detection device for detecting substances. Relates to the field of substance component detection, and the method comprises the following steps: determining a to-be-detected partition in a chip; aligning a laser source in the detection equipment to the subarea to be detected; and sequentially carrying out substance detection on the to-be-detected subareas through detection equipment. The method, the device, the chip and the detection equipment for detecting the substance can improve the detection efficiency of Raman detection and save the detection cost.
Description
Technical Field
The invention relates to the field of computer information processing, in particular to a method, a device, a chip and detection equipment for detecting substances.
Background
The Raman spectroscopy is an analysis method for analyzing a scattering spectrum with a frequency different from that of incident light to obtain information on molecular vibration and rotation based on a Raman scattering effect found by indian scientists c.v. Raman (man), and is applied to molecular structure research. The Raman spectrum analysis method does not need to carry out pretreatment on the sample, does not have the preparation process of the sample, avoids the generation of errors, and has the advantages of simple and convenient operation, short determination time, high sensitivity and the like in the analysis process.
The Surface Enhanced Raman Scattering (SERS) effect refers to a phenomenon that in a specially prepared metal good conductor surface or sol, in an excitation area, a raman scattering signal of an adsorbed molecule is greatly enhanced compared with a common raman scattering (NRS) signal due to enhancement of an electromagnetic field on the surface or near the surface of a sample. The surface enhanced Raman overcomes the defect of low Raman spectrum sensitivity, can obtain structural information which is not easily obtained by the conventional Raman spectrum, is widely used for surface research, adsorption interface surface state research, interface orientation and configuration of biological large and small molecules, conformation research, structural analysis and the like, and can effectively analyze the adsorption orientation, adsorption state change, interface information and the like of a compound on an interface.
Currently, raman detection is widely used in the market, and raman-enhanced chips for detecting low-concentration substances, such as pesticide residues and cell detection, also begin to be applied in a small range. The Surface Enhanced Raman Scattering (SERS) technology overcomes the inherent weak signal of the traditional Raman spectrum, can increase the Raman signal intensity by several orders of magnitude, and enables low-concentration substances to be detected sufficiently. However, the raman enhanced chip is formed by attaching a nano material to a silicon wafer or a quartz wafer, and is currently expensive. Moreover, for raman detection, if continuous multi-sample detection is required, a new raman amplification chip is frequently replaced, thereby reducing the raman detection efficiency. The manufacturing cost of the Raman chip is high, the detection efficiency of the Raman detection equipment is low, and the Raman chip is a technical problem which is urgently needed to be solved at present.
Therefore, a new method, device, chip and detection apparatus for substance detection are needed.
The above information disclosed in this background section is only for enhancement of understanding of the background of the invention and therefore it may contain information that does not constitute prior art that is already known to a person of ordinary skill in the art.
Disclosure of Invention
In view of this, the present invention provides a method, an apparatus, a chip and a detection device for detecting a substance, which can improve the detection efficiency of raman detection and save the detection cost.
Additional features and advantages of the invention will be set forth in the detailed description which follows, or may be learned by practice of the invention.
According to a first aspect of the present invention, there is provided a method for substance detection, the method comprising: determining a to-be-detected partition in a chip; aligning a laser source in the detection equipment to the subarea to be detected; and sequentially carrying out substance detection on the to-be-detected subareas through detection equipment.
According to a second aspect of the present invention, there is provided a device for substance detection, the device comprising: the partitioning module is used for determining a partition to be detected in the chip; the adjusting module is used for aligning a laser source in the detection equipment to the subarea to be detected; and the detection module is used for sequentially detecting the substances of the to-be-detected subareas through detection equipment.
According to a third aspect of the present invention, there is provided a chip for substance detection, the chip comprising: a lower substrate, a middle substrate and an upper substrate; the upper substrate is used for bearing a substance to be detected, and is divided into a plurality of subareas through raised separation lines.
According to a fourth aspect of the present invention, there is provided a detection apparatus for substance detection, the detection apparatus comprising: a chip placement platform; a laser source; the laser source and the chip placing platform can move relatively, and the laser source can detect any partition on the chip through the relative movement.
According to a fifth aspect of the present invention, there is provided an electronic apparatus comprising: one or more processors; a storage device arranged to store one or more programs; when executed by one or more processors, cause the one or more processors to implement a method as above.
According to a sixth aspect of the invention, a computer-readable medium is proposed, on which a computer program is stored which, when being executed by a processor, carries out the method as in the above.
According to the method, the device, the chip and the detection equipment for detecting the substance, the detection efficiency of Raman detection can be improved, and the detection cost is saved.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.
Drawings
Fig. 1 is a prior art illustration of a raman-enhanced chip.
FIG. 2 is a schematic illustration of a partition of a chip for substance detection, according to an exemplary embodiment.
FIG. 3 is a flow chart illustrating a method for substance detection according to an exemplary embodiment.
FIG. 4 is a schematic diagram illustrating a center of a chip in a method for substance detection according to an exemplary embodiment.
FIG. 5 is a schematic view of a laser head rotation angle in a method for substance detection according to one exemplary embodiment.
FIG. 6 is a flow chart illustrating a method for substance detection according to another exemplary embodiment.
Fig. 7 is a schematic diagram illustrating a chip measurement result presentation in a method for substance detection according to an exemplary embodiment.
FIG. 8 is a block diagram illustrating an apparatus for substance detection according to an exemplary embodiment.
FIG. 9 is a block diagram illustrating an electronic device in accordance with an example embodiment.
FIG. 10 schematically illustrates a computer-readable storage medium in an exemplary embodiment of the disclosure.
Detailed Description
Example embodiments will now be described more fully with reference to the accompanying drawings. Example embodiments may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of example embodiments to those skilled in the art. The same reference numerals denote the same or similar parts in the drawings, and thus, a repetitive description thereof will be omitted.
Furthermore, the described features, structures, or characteristics may be combined in any suitable manner in one or more embodiments. In the following description, numerous specific details are provided to provide a thorough understanding of embodiments of the invention. One skilled in the relevant art will recognize, however, that the invention may be practiced without one or more of the specific details, or with other methods, components, devices, steps, and so forth. In other instances, well-known methods, devices, implementations or operations have not been shown or described in detail to avoid obscuring aspects of the invention.
The block diagrams shown in the figures are functional entities only and do not necessarily correspond to physically separate entities. I.e. these functional entities may be implemented in the form of software, or in one or more hardware modules or integrated circuits, or in different networks and/or processor means and/or microcontroller means.
The flow charts shown in the drawings are merely illustrative and do not necessarily include all of the contents and operations/steps, nor do they necessarily have to be performed in the order described. For example, some operations/steps may be decomposed, and some operations/steps may be combined or partially combined, so that the actual execution sequence may be changed according to the actual situation.
It will be appreciated by those skilled in the art that the drawings are merely schematic representations of exemplary embodiments, and that the blocks or flow charts in the drawings are not necessarily required to practice the present invention and are, therefore, not intended to limit the scope of the present invention.
FIG. 1 is a prior art illustration of a Raman enhanced chip. In the prior art, a Raman enhancement chip is not partitioned, and one Raman enhancement detection chip can only carry out one-time substance detection. FIG. 2 is a schematic illustration of a partition of a chip for substance detection according to an exemplary embodiment
Wherein, the chip for detecting the substance can be a Raman enhanced chip, and the chip comprises: a lower substrate, a middle substrate and an upper substrate; the upper substrate is used for bearing a substance to be detected, and is divided into a plurality of subareas through raised separation lines.
In one embodiment, the chip for substance detection of the present application may employ any form of partitioning, preferably, an equal partitioning manner.
In one embodiment, the internal raman-enhanced partition splitting may be different when the off-chip dimensions are identical. The partition between the partitions can be, for example, a raised partition line to prevent the liquid in one partition from being excessive and passing to other partitions, so that the detection result is wrong.
According to an aspect of the present invention, there is provided a detection apparatus for detection of a substance, the detection apparatus comprising: a chip placement platform; a laser source; the laser source and the chip placing platform can move relatively, and the laser source can detect any partition on the chip through the relative movement. The raman-enhanced chip may for example be inserted inside the detection device, and the focal length of the laser of the detection device after insertion is in the plane in which the raman-enhanced chip is located. And the laser focus position can be positioned on each subarea in turn through the translational motion of the mechanical structure or the change of the laser beam angle.
In one embodiment, the method of moving the laser source and the chip placement stage relative to each other includes: the laser source is fixed in position, and the chip is translated; or the chip fixing position, the laser source translation mode; or the chip fixing position, and the angle of the light beam of the laser source is changed.
FIG. 3 is a flow chart illustrating a method for substance detection according to an exemplary embodiment.
As shown in fig. 3, in S302, a to-be-detected partition in the chip is determined. Wherein, the chip includes: a Raman enhancement chip. As an example in fig. 2, the raman-enhanced chip in the present application may for example comprise a plurality of partitions. In one embodiment, before determining the to-be-detected partition in the chip, the method further includes: the chip is used for bearing at least one substance to be detected, the Raman enhanced chip can adopt any form of partition, the area of one enhanced chip is divided into a plurality of partitions, and a plurality of substances to be detected (one substance to be detected in one partition) are respectively coated or dripped. Further comprising: and acquiring the distribution information of the partitions in the chip according to the identification of the chip.
In S304, a laser source in the inspection apparatus is directed at the partition to be inspected. A detection apparatus, comprising: a Raman detection device. Wherein, aim at the subregion of waiting to detect laser source in the check out test set, include: the laser source is aligned to the subarea to be detected in a mode of fixing the position by the laser source and horizontally moving the chip; or the laser source is aligned to the subarea to be detected in a translation mode through the chip fixing position; or the laser source is aligned to the subarea to be detected in a mode that the chip is fixed at the position and the angle of the light beam of the laser source is changed.
In S306, the substance detection is sequentially performed on the to-be-detected partitions by using a detection device. The set of coordinate points may be generated, for example, from the center coordinates of the subarea to be detected, and when there are a plurality of subareas to be detected, the substance detection may be performed sequentially from any coordinate point in the set of coordinate points. The sequence of the substance detection may be further specified by a user, or the optimized path selection may be performed according to the positions of the chip partitions and the path to be moved by the laser head, and the substance detection may be performed sequentially according to the optimized path, which is not limited by the present invention. In one embodiment, the detecting the substance of the partition to be detected in the chip by a detecting device according to the distribution information of the partition includes: sequentially determining the center coordinates of the subareas to be detected according to the distribution information of the subareas; and the laser source in the detection equipment is sequentially aligned with the central coordinates of the subareas to be detected, and the subareas to be detected are sequentially subjected to substance detection. Wherein, the distribution information of the subareas comprises: the arrangement mode of each partition in the chip and the center point coordinate of each partition in the chip.
According to the method for detecting the substances, disclosed by the invention, the Raman enhancement chip with the plurality of subareas bears the plurality of substances, and the laser head of the detection equipment is adjusted to sequentially align the laser head with each subarea on the chip, so that the substances in the subareas are sequentially detected, the detection efficiency of the Raman detection can be improved, and the detection cost is saved.
In an exemplary embodiment of the present disclosure, acquiring distribution information of partitions in the chip according to the identifier of the chip includes: acquiring the distribution information of the partitions in the chip through a storage area in the chip according to the identification of the chip; or acquiring the distribution information of the partitions in the chip through a storage area in the detection equipment according to the identifier of the chip; or acquiring the distribution information of the partitions in the chip at the cloud according to the identification of the chip. The peripheral dimensions of the raman-enhanced chip are generally fixed for the same device, as long as the specifications of the chip can be identified when the raman-enhanced chip is inserted. For example, by reading the ID number of the Raman enhanced chip, 9 partitions of the chip and the coordinate position of the center point of each partition are obtained. For example, the partition number and the central point coordinate position of each partition are read through the embedded information of the chip, or only the ID number is read, and the partition number of the raman enhancement chip corresponding to the ID number and the central point coordinate position of each partition are obtained according to the ID number in the local or cloud terminal. Because the same total number of partitions can also have different partitioning methods, in the application, the raman enhancement chips with different specifications are all endowed with different ID numbers, so that the detection equipment can correctly distinguish the partitions on the chip.
In an exemplary embodiment of the disclosure, the determining the to-be-detected partition in the chip includes: determining a to-be-detected partition in the chip according to a user instruction; or determining the to-be-detected partition in the chip according to the recognition result of the chip by the detection equipment. Determining the to-be-detected partition in the chip according to a user instruction, wherein the determining comprises the following steps: generating partition display information according to the distribution information of the partitions; displaying the partition display information at a display end; and determining the partition to be detected by the user according to the prompt of the partition display information.
FIG. 4 is a schematic diagram illustrating a center of a chip in a method for substance detection according to an exemplary embodiment. FIG. 5 is a schematic view of a laser head rotation angle in a method for substance detection according to one exemplary embodiment. Three ways of directing the laser source in the detection device at the partition to be detected are illustrated by fig. 4 and 5.
The first method is as follows: and fixing the position by the laser source, and horizontally moving the chip to enable the laser source to be aligned with the partition to be detected. For example, the laser path is fixed, the laser focus is fixed, and the fixing device of the raman enhancement chip can perform in-plane translation through the mechanical structure, so that the laser focus irradiates each partition of the raman enhancement chip, as shown in fig. 4, taking a 9-partition raman enhancement chip as an example, assuming that the laser focus is directly opposite to the center of the enhancement chip after the enhancement chip is installed. The exact center is taken as the origin of coordinates, the horizontal direction is taken as the X axis, and the vertical direction is taken as the Y axis.
Assuming that the central coordinate point of the first zone on the upper left in the 9-zone raman enhancement chip illustrated in fig. 4 is (-2.5, 2), it can be seen that the mechanical structure carrying the raman enhancement chip can just irradiate the center position of the zone 1 with the laser focus by moving 2.5 to the X-axis positive axis and 2 to the Y-axis negative axis, that is, the moving coordinates of the mechanical structure are (2.5, -2). Other partitions and Raman enhancement chips with other specifications have the same reason. Therefore, the detection of each partitioned substance can be completed in sequence by moving the mechanical structure to sequentially bring each partition into the focal point of the laser.
The second method comprises the following steps: and the laser source is aligned to the subarea to be detected in a translation mode through the chip fixing position. For example, the laser head is moved by a mechanical structure in a plane parallel to the raman enhancement chip, which is stationary, such that the laser focus is sequentially applied to each of the sections of the raman enhancement chip. In this way, the same as the implementation process of the above first equation, only the laser head translates along the X axis and the Y axis, and assuming that the central coordinate point of the first upper left partition in the 9-partition raman enhancement chip illustrated in fig. 4 is (-2.5, 2), it can be seen that the mechanical structure carrying the laser head can just irradiate the central position of the partition 1 by moving 2.5 towards the negative X axis and 2 towards the positive Y axis, that is, the moving coordinate of the mechanical structure is (-2.5, 2). Other partitions and Raman enhancement chips with other specifications have the same reason.
The third method comprises the following steps: and the laser source is aligned to the subarea to be detected in a mode of changing the angle of the light beam of the laser source through the chip fixing position. For example, the raman-enhanced chip is stationary and the laser head/beam can be angled so that the laser focus illuminates each zone in the raman-enhanced chip in turn. This may be done, for example, by optical or mechanical structures that enable the laser beam to be focused or nearly focused on each region of the raman enhancement chip. Assuming that the coordinates of the central point of a certain partition on the raman enhancement chip of the certain multi-partition are (X, Y), the rotation angle of the laser beam along the X axis is α, the rotation angle along the Y axis is β, and the vertical distance from the laser lens to the raman enhancement chip is d, it is schematically shown in fig. 5 below.
According to the geometrical relationship, the following steps are carried out:
tanα＝x/d；
tanβ＝y/d；
then, one can solve:
α＝arctan(x/d)；
β＝arctan(y/d)；
after the coordinates (X, Y) of the center point of a certain partition are obtained, the laser beam only needs to rotate the arctan (X/d) along the X axis and rotate the arctan (Y/d) along the Y axis, and then the laser focus can be irradiated to the center point of the partition. Optionally, the laser lens further performs, for example, automatic focal length adjustment, so that the laser focus can be more accurately focused on each partition.
FIG. 6 is a flow chart illustrating a method for substance detection according to another exemplary embodiment. Fig. 6 is an exemplary illustration of a method for substance detection, and fig. 6 details different chip ID acquisition modes and result presentation processes.
Wherein, in S602, the user applies or drops one or more substances to be detected in one or more regions of the raman enhancement chip, and only one sample in each region is kept.
In S604, the raman-enhanced chip is inserted, and the device is turned on in preparation for starting detection.
In S606, the detection device detects the inserted raman-enhanced chip, and if the coordinate information is completely read from the chip, the process goes to step 608; if the local or cloud corresponding mode is adopted, go to step 610.
In S608, the detection device reads the ID number, the partition manner, and the center point coordinate information of each partition from the enhanced chip.
In S610, the detection device reads the ID number from the turbo chip, and searches for the type of the turbo chip corresponding to the ID number in the local and/or cloud terminal of the terminal according to the ID number, which mainly includes a partition mode and coordinate information of a center point of each partition, and may also include a partition diagram of the raman turbo chip.
In S612, the detection device displays a partition diagram of the enhancement chip on a screen, where the diagram corresponds to the partition of the enhancement chip.
In S614, the user may check one or more partitions that need to be detected this time. A9-partition enhanced chip may only use any portion of the partitions, such as 5 partitions, at a time, and then click to start detection.
In S616, the partition selected by the user is obtained and may correspond to the coordinate point of the selected partition. And sending a detection instruction and coordinate point information to the detection device from any coordinate point in the coordinate point set.
In S618, according to one of the three implementations corresponding to the detection device, the laser focus is positioned at the coordinate point by the translational motion of the mechanical structure or the change of the laser beam angle, the raman spectrum starts to be collected, and after the collection is finished (the signal-to-noise ratio reaches the standard), the raman spectrum is recorded and matched in the database.
In S620, if there is a coordinate point to be detected next in the coordinate point set, a detection instruction and coordinate point information are sent to the detection device, and the process goes to step 618.
In S622, the detection result is fed back to the user, and the flow ends. A results presentation interface such as that shown in fig. 7 may be presented on the screen, for example.
According to the method for detecting the substance, a piece of the enhanced chip can support a plurality of partitions in various modes through the structure of the specific Raman enhanced chip and the detection equipment of the specific Raman enhanced chip. In the aspect of the detection device, the Raman enhancement chip can be inserted into the machine of the detection device, and the focal distance of the laser after insertion is just in the plane of the Raman enhancement chip. The partition mode and the coordinate point information of each partition are matched by reading the ID number of the enhanced chip, and then the laser focus position can be automatically and sequentially positioned on one or more partitions selected by a user by detecting the translation motion of a mechanical structure of the equipment or the change of the angle of a laser beam, so that the material detection of each partition is sequentially finished. According to the method for detecting the substance, the use cost of the Raman enhancement chip can be reduced, the detection efficiency of continuous multi-substance detection is improved, and the user experience is greatly improved.
The following are embodiments of the apparatus of the present invention that may be used to perform embodiments of the method of the present invention. For details which are not disclosed in the embodiments of the apparatus of the present invention, reference is made to the embodiments of the method of the present invention.
FIG. 8 is a block diagram illustrating an apparatus for substance detection according to an exemplary embodiment. The device 80 for substance detection may for example comprise: a partitioning module 802, an adjusting module 804, and a detecting module 806.
The partition module 802 is configured to determine a partition to be detected in the chip. The chip, wherein the chip includes: a Raman enhancement chip. As described above, the raman-enhanced chip in the present application may, for example, include a plurality of partitions. In one embodiment, before determining the to-be-detected partition in the chip, the method further includes: the chip is used for bearing at least one substance to be detected, the Raman enhanced chip can adopt any form of partition, the area of one enhanced chip is divided into a plurality of partitions, and a plurality of substances to be detected (one substance to be detected in one partition) are respectively coated or dripped. Further comprising: and acquiring the distribution information of the partitions in the chip according to the identification of the chip.
The adjustment module 804 is configured to align a laser source in the inspection apparatus with the partition to be inspected. Wherein, check out test set includes: a Raman detection device. Wherein, aim at the subregion of waiting to detect laser source in the check out test set, include: the laser source is aligned to the subarea to be detected in a mode of fixing the position by the laser source and horizontally moving the chip; or the laser source is aligned to the subarea to be detected in a translation mode through the chip fixing position; or the laser source is aligned to the subarea to be detected in a mode that the chip is fixed at the position and the angle of the light beam of the laser source is changed.
The detection module 806 is configured to sequentially perform substance detection on the to-be-detected partitions by the detection device. The set of coordinate points may be generated, for example, from the center coordinates of the subarea to be detected, and when there are a plurality of subareas to be detected, the substance detection may be performed sequentially from any coordinate point in the set of coordinate points. The sequence of the substance detection may be further specified by a user, or the optimized path selection may be performed according to the positions of the chip partitions and the path to be moved by the laser head, and the substance detection may be performed sequentially according to the optimized path, which is not limited by the present invention.
The apparatus 80 for substance detection may further include, for example, a partition detection module (not shown in the figure) for obtaining distribution information of partitions in the chip according to the chip identifier, which may include: acquiring the distribution information of the partitions in the chip through a storage area in the chip according to the identification of the chip; or acquiring the distribution information of the partitions in the chip through a storage area in the detection equipment according to the identifier of the chip; or acquiring the distribution information of the partitions in the chip at the cloud according to the identification of the chip. The peripheral dimensions of the raman-enhanced chip are generally fixed for the same device, as long as the specifications of the chip can be identified when the raman-enhanced chip is inserted. For example, by reading the ID number of the Raman enhanced chip, 9 partitions of the chip and the coordinate position of the center point of each partition are obtained. For example, the partition number and the central point coordinate position of each partition are read through the embedded information of the chip, or only the ID number is read, and the partition number of the raman enhancement chip corresponding to the ID number and the central point coordinate position of each partition are obtained according to the ID number in the local or cloud terminal. Because the same total number of partitions can also have different partitioning methods, in the application, the raman enhancement chips with different specifications are all endowed with different ID numbers, so that the detection equipment can correctly distinguish the partitions on the chip.
The means 80 for substance detection may also comprise, for example, a partition-determining module (not shown in the figures) for determining, according to user instructions, the partition to be detected in the chip; or determining the to-be-detected partition in the chip according to the recognition result of the chip by the detection equipment. Determining the to-be-detected partition in the chip according to a user instruction, wherein the determining comprises the following steps: generating partition display information according to the distribution information of the partitions; displaying the partition display information at a display end; and determining the partition to be detected by the user according to the prompt of the partition display information.
According to the device for detecting the substances, the Raman enhancement chip with the plurality of subareas bears the plurality of substances, the laser head of the detection equipment is adjusted to be sequentially aligned with each subarea on the chip, and the substances in the subareas are sequentially detected, so that the detection efficiency of the Raman detection can be improved, and the detection cost is saved.
FIG. 9 is a block diagram illustrating an electronic device in accordance with an example embodiment.
An electronic device 200 according to this embodiment of the invention is described below with reference to fig. 9. The electronic device 200 shown in fig. 9 is only an example, and should not bring any limitation to the functions and the scope of use of the embodiments of the present invention.
As shown in fig. 9, the electronic device 200 is embodied in the form of a general purpose computing device. The components of the electronic device 200 may include, but are not limited to: at least one processing unit 210, at least one memory unit 220, a bus 230 connecting different system components (including the memory unit 220 and the processing unit 210), a display unit 240, and the like.
Wherein the storage unit stores program code executable by the processing unit 210 to cause the processing unit 210 to perform the steps according to various exemplary embodiments of the present invention described in the above-mentioned electronic prescription flow processing method section of the present specification. For example, the processing unit 210 may perform the steps as shown in fig. 3 or fig. 6.
The memory unit 220 may include readable media in the form of volatile memory units, such as a random access memory unit (RAM)2201 and/or a cache memory unit 2202, and may further include a read only memory unit (ROM) 2203.
The storage unit 220 may also include a program/utility 2204 having a set (at least one) of program modules 2205, such program modules 2205 including, but not limited to: an operating system, one or more application programs, other program modules, and program data, each of which, or some combination thereof, may comprise an implementation of a network environment.
The electronic device 200 may also communicate with one or more external devices 300 (e.g., keyboard, pointing device, bluetooth device, etc.), with one or more devices that enable a user to interact with the electronic device 200, and/or with any devices (e.g., router, modem, etc.) that enable the electronic device 200 to communicate with one or more other computing devices. Such communication may occur via an input/output (I/O) interface 250. Also, the electronic device 200 may communicate with one or more networks (e.g., a Local Area Network (LAN), a Wide Area Network (WAN), and/or a public network such as the Internet) via the network adapter 260. The network adapter 260 may communicate with other modules of the electronic device 200 via the bus 230. It should be appreciated that although not shown in the figures, other hardware and/or software modules may be used in conjunction with the electronic device 200, including but not limited to: microcode, device drivers, redundant processing units, external disk drive arrays, RAID systems, tape drives, and data backup storage systems, among others.
Through the above description of the embodiments, those skilled in the art will readily understand that the exemplary embodiments described herein may be implemented by software, or by software in combination with necessary hardware. Therefore, the technical solution according to the embodiments of the present disclosure may be embodied in the form of a software product, which may be stored in a non-volatile storage medium (which may be a CD-ROM, a usb disk, a removable hard disk, etc.) or on a network, and includes several instructions to enable a computing device (which may be a personal computer, a server, or a network device, etc.) to execute the processing method according to the embodiments of the present disclosure.
FIG. 10 schematically illustrates a computer-readable storage medium in an exemplary embodiment of the disclosure.
Referring to fig. 10, a program product 400 configured to implement the above method according to an embodiment of the present invention is described, which may employ a portable compact disc read only memory (CD-ROM) and include program code, and may be run on a terminal device, such as a personal computer. However, the program product of the present invention is not limited in this regard and, in the present document, a readable storage medium may be any tangible medium that can contain, or store a program for use by or in connection with an instruction execution system, apparatus, or device.
The computer readable medium carries one or more programs which, when executed by a device, cause the computer readable medium to perform the functions of: determining a to-be-detected partition in a chip; aligning a laser source in the detection equipment to the subarea to be detected; and sequentially carrying out substance detection on the to-be-detected subareas through detection equipment.
In addition, the structures, the proportions, the sizes, and the like shown in the drawings of the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used for limiting the limit conditions which the present disclosure can implement, so that the present disclosure has no technical essence, and any modification of the structures, the change of the proportion relation, or the adjustment of the sizes, should still fall within the scope which the technical contents disclosed in the present disclosure can cover without affecting the technical effects which the present disclosure can produce and the purposes which can be achieved. In addition, the terms "above", "first", "second" and "a" as used in the present specification are for the sake of clarity only, and are not intended to limit the scope of the present disclosure, and changes or modifications of the relative relationship may be made without substantial technical changes and modifications.
Claims (9)
1. A method for substance detection, comprising:
receiving at least one substance to be detected through a chip, wherein the chip comprises a plurality of partitions for bearing the substance to be detected;
acquiring distribution information of partitions in the chip according to the identification of the chip, wherein the distribution information of the partitions comprises: the arrangement mode of each partition in the chip and the center point coordinate of each partition in the chip;
generating partition display information according to the distribution information of the partitions;
displaying the partition display information at a display end;
the user determines the to-be-detected partition according to the prompt of the partition display information;
aligning a laser source in the detection equipment to the subarea to be detected;
sequentially determining the center coordinates of the subareas to be detected according to the distribution information of the subareas; and
and the laser source in the detection equipment is sequentially aligned with the central coordinates of the subareas to be detected, and the subareas to be detected are sequentially subjected to substance detection.
2. The method of claim 1, the chip, comprising:
a Raman enhancement chip.
3. The method of claim 1, the detection device, comprising:
a Raman detection device.
4. The method of claim 1, wherein the obtaining distribution information of the partitions in the chip according to the identifier of the chip comprises:
acquiring the distribution information of the partitions in the chip through a storage area in the chip according to the identification of the chip; or
Acquiring the distribution information of the partitions in the chip through a storage area in the detection equipment according to the identification of the chip; or
And acquiring the distribution information of the partitions in the chip at the cloud according to the identification of the chip.
5. The method of claim 1, wherein aiming a laser source in a detection device at the partition to be detected comprises:
the laser source is aligned to the subarea to be detected in a mode of fixing the position by the laser source and horizontally moving the chip; or
Through the chip fixing position and the translation mode of the laser source, the laser source is aligned to the subarea to be detected; or
And the laser source is aligned to the subarea to be detected in a mode of changing the angle of the light beam of the laser source through the chip fixing position.
6. The method of claim 5, wherein the laser source is directed at the partition to be inspected by angularly shifting a beam of the laser source through the chip-fixing location, further comprising:
and the laser source performs automatic focus adjustment processing.
7. An apparatus for substance detection, comprising:
a partition detection module, configured to obtain distribution information of partitions in a chip according to an identifier of the chip, where the distribution information of the partitions includes: the arrangement mode of each partition in the chip and the center point coordinate of each partition in the chip; the chip comprises a plurality of partitions for bearing the substances to be detected;
the partition determining module is set to generate partition display information according to the distribution information of the partitions; displaying the partition display information at a display end; the user determines the to-be-detected partition according to the prompt of the partition display information;
the adjusting module is used for aligning a laser source in the detection equipment to the subarea to be detected; and
the detection module is arranged for sequentially determining the center coordinates of the subareas to be detected according to the distribution information of the subareas; and the laser source in the detection equipment is sequentially aligned with the central coordinates of the subareas to be detected, and the subareas to be detected are sequentially subjected to substance detection.
8. An electronic device, comprising:
one or more processors;
a storage device arranged to store one or more programs;
when executed by the one or more processors, cause the one or more processors to implement the method of any one of claims 1-6.
9. A computer-readable medium, on which a computer program is stored which, when being executed by a processor, carries out the method according to any one of claims 1-6.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2017/119089 WO2019127130A1 (en) | 2017-12-27 | 2017-12-27 | Method, apparatus, chip and detection device for substance detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108700522A CN108700522A (en) | 2018-10-23 |
| CN108700522B true CN108700522B (en) | 2021-03-09 |
Family
ID=63844020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780002409.9A Active CN108700522B (en) | 2017-12-27 | 2017-12-27 | Method, device, chip and detection equipment for detecting substance |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN108700522B (en) |
| WO (1) | WO2019127130A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110174391B (en) * | 2019-05-29 | 2022-03-29 | 深圳前海达闼云端智能科技有限公司 | Position deviation correction method, device, storage medium and electronic equipment |
| CN110793957A (en) * | 2019-11-27 | 2020-02-14 | 拱北海关技术中心 | Distributed customs field inspection Raman spectrum system based on cloud platform technology |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4030827A (en) * | 1973-12-03 | 1977-06-21 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Apparatus for the non-destructive examination of heterogeneous samples |
| US6067154A (en) * | 1998-10-23 | 2000-05-23 | Advanced Micro Devices, Inc. | Method and apparatus for the molecular identification of defects in semiconductor manufacturing using a radiation scattering technique such as raman spectroscopy |
| DE102005031140A1 (en) * | 2005-07-04 | 2007-01-18 | Erwin Kayser-Threde Gmbh | Material e.g. bioparticle, identifying method e.g. for stone sample, involves identifying material condition of surface in selected places of surface based on Raman spectrum, which is produced on selected places based on image of surface |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003007792A (en) * | 2001-06-27 | 2003-01-10 | Seiko Epson Corp | Semiconductor analyzer, method of analyzing semiconductor and method of manufacturing semiconductor device |
| CN102183505A (en) * | 2011-02-18 | 2011-09-14 | 王小倩 | Analysing system of special detecting chip for array type micro-fluidic surface enhanced Raman scattering (SERS) |
| CN102706835A (en) * | 2012-05-14 | 2012-10-03 | 中央民族大学 | Sensing chip of dual-detecting biochemical sensing detector and preparation method thereof |
| CN104406955B (en) * | 2014-12-08 | 2017-11-24 | 山东博济医药科技有限公司 | A kind of substance detecting method based on Raman spectrum |
| CN104515765A (en) * | 2014-12-26 | 2015-04-15 | 江苏物联网研究发展中心 | Microfluidic surface-enhanced Raman scattering transparent device structure and preparation method thereof |
| CN105772118B (en) * | 2015-10-22 | 2017-09-12 | 重庆大学 | The SERS micro-fluidic chips and preparation method of integrated enhancing substrate on ITO electro-conductive glass |
-
2017
- 2017-12-27 WO PCT/CN2017/119089 patent/WO2019127130A1/en active Application Filing
- 2017-12-27 CN CN201780002409.9A patent/CN108700522B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4030827A (en) * | 1973-12-03 | 1977-06-21 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Apparatus for the non-destructive examination of heterogeneous samples |
| US6067154A (en) * | 1998-10-23 | 2000-05-23 | Advanced Micro Devices, Inc. | Method and apparatus for the molecular identification of defects in semiconductor manufacturing using a radiation scattering technique such as raman spectroscopy |
| DE102005031140A1 (en) * | 2005-07-04 | 2007-01-18 | Erwin Kayser-Threde Gmbh | Material e.g. bioparticle, identifying method e.g. for stone sample, involves identifying material condition of surface in selected places of surface based on Raman spectrum, which is produced on selected places based on image of surface |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108700522A (en) | 2018-10-23 |
| WO2019127130A1 (en) | 2019-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11768136B2 (en) | Systems and methods for meso-dissection | |
| US8508588B2 (en) | Methods and systems for identifying well wall boundaries of microplates | |
| JP6310501B2 (en) | Multi cuvette automatic sampler for optical measurement | |
| CN108700522B (en) | Method, device, chip and detection equipment for detecting substance | |
| CN107992484B (en) | Method, device and storage medium for evaluating performance of OCR system | |
| Lee et al. | Molecular tissue profiling by MALDI imaging: Recent progress and applications in cancer research | |
| JP2018510327A (en) | System and method for mesodai section | |
| JP2010175291A (en) | Liquid level detection device and method | |
| WO2017179145A1 (en) | Charged particle beam device and sample holder | |
| TWI479145B (en) | System and Method for Detecting Shape Flaw | |
| CN109297952B (en) | Rice paper quality identification system based on laser-induced breakdown spectroscopy technology | |
| US9239318B2 (en) | Spectroscopic sample inspection for automated chromatography | |
| CN104297256B (en) | Apparent flaws detecting system and method | |
| CN109596639A (en) | Defect detecting system and defect inspection method | |
| CN109884082B (en) | Method for detecting smooth surface defects | |
| Heeren | Proteome imaging: a closer look at life's organization | |
| JP2002243652A (en) | Method and apparatus for specifying dust, and method for specifying dust source | |
| US11913922B2 (en) | Techniques for targeted compound analysis | |
| Blow | Mass spectrometry and proteomics: hitting the mark | |
| JP2019045399A (en) | Inspection method, inspection program and inspection apparatus | |
| CN220626214U (en) | Exfoliated cell finding instrument | |
| JP2005291795A (en) | Fluorescence detection device and image processing system | |
| CN210199119U (en) | Medical rotating disc type multi-project fluorescence immunoassay analyzer | |
| CN115876805A (en) | Visual detection device and detection method | |
| WO2021203291A1 (en) | Lens-free microscopic imaging system and method, and biochemical substance detection system and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220530 Address after: 1904-010, 20th floor, building 4, No. 11, Changchun Bridge Road, Haidian District, Beijing 100089 Patentee after: BEIJING CLOUDOPTEK TECHNOLOGY Co.,Ltd. Address before: 518000 Room 201, building A, No. 1, Qian Wan Road, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong (Shenzhen Qianhai business secretary Co., Ltd.) Patentee before: CLOUDMINDS (SHENZHEN) HOLDINGS Co.,Ltd. |
|
| TR01 | Transfer of patent right |